##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937742_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 354962 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.238214231382514 33.0 31.0 34.0 31.0 34.0 2 32.44401090820989 34.0 31.0 34.0 31.0 34.0 3 32.490167961640964 34.0 31.0 34.0 31.0 34.0 4 36.12586699421347 37.0 35.0 37.0 35.0 37.0 5 36.04515412917439 37.0 35.0 37.0 35.0 37.0 6 36.10111223173185 37.0 35.0 37.0 35.0 37.0 7 36.0471825153115 37.0 35.0 37.0 35.0 37.0 8 36.024974504313136 37.0 35.0 37.0 35.0 37.0 9 37.69877902423358 39.0 37.0 39.0 35.0 39.0 10-11 37.6299040460669 39.0 37.0 39.0 35.0 39.0 12-13 37.524512483026356 39.0 37.0 39.0 35.0 39.0 14-15 38.680985570286396 40.0 38.0 41.0 34.0 41.0 16-17 38.64650441455705 40.0 38.0 41.0 34.5 41.0 18-19 38.69134019979603 40.0 38.0 41.0 34.0 41.0 20-21 38.6190944382779 40.0 38.0 41.0 34.0 41.0 22-23 38.47125748671689 40.0 38.0 41.0 34.0 41.0 24-25 38.49947881745088 40.0 38.0 41.0 34.0 41.0 26-27 38.341192860080795 40.0 38.0 41.0 34.0 41.0 28-29 38.17939244200788 40.0 38.0 41.0 34.0 41.0 30-31 38.16980127450262 40.0 37.5 41.0 34.0 41.0 32-33 38.02117832331348 40.0 37.0 41.0 34.0 41.0 34-35 37.86303604329477 40.0 37.0 41.0 33.0 41.0 36-37 37.66220609530034 40.0 36.5 41.0 33.0 41.0 38-39 37.484654695432184 40.0 36.0 41.0 33.0 41.0 40-41 37.25974752226999 40.0 36.0 41.0 33.0 41.0 42-43 36.9873916081158 40.0 36.0 41.0 31.5 41.0 44-45 36.54006203480936 39.0 35.0 41.0 30.5 41.0 46-47 36.011127951724404 39.0 35.0 41.0 29.5 41.0 48-49 35.519480958525136 39.0 35.0 41.0 27.5 41.0 50-51 35.216417532017516 39.0 35.0 40.5 26.0 41.0 52-53 34.928917743307736 39.0 35.0 40.0 25.5 41.0 54-55 34.67130425228616 38.0 34.5 40.0 24.0 41.0 56-57 34.3859032797877 38.0 34.0 40.0 23.5 41.0 58-59 34.10501828364727 38.0 34.0 40.0 23.0 41.0 60-61 33.74479380891476 37.5 33.5 40.0 22.0 41.0 62-63 33.37240324316406 37.0 33.0 40.0 20.5 41.0 64-65 32.96138459891481 36.5 33.0 39.0 18.5 41.0 66-67 32.51878088358754 36.0 32.0 39.0 16.5 40.0 68-69 32.06257712093125 35.0 32.0 39.0 12.5 40.0 70-71 31.63194933542182 35.0 31.0 38.0 8.0 40.0 72-73 31.133118474653628 35.0 31.0 37.0 7.0 39.5 74-75 30.61494610690722 34.5 30.0 37.0 4.0 39.0 76-77 29.40174300347643 33.5 28.5 35.5 2.0 38.0 78-79 29.99637425977992 34.0 30.0 36.0 2.0 38.0 80-81 29.78781954124667 34.0 30.0 35.5 2.0 37.0 82-83 29.437107352336305 34.0 30.0 35.0 2.0 37.0 84-85 29.0491052563373 34.0 29.0 35.0 2.0 36.5 86-87 28.61578704199323 34.0 29.0 35.0 2.0 36.0 88-89 28.264462111437282 34.0 29.0 35.0 2.0 35.5 90-91 27.746474270485294 33.5 27.0 35.0 2.0 35.0 92-93 27.252522805258025 33.0 26.5 35.0 2.0 35.0 94-95 26.748861850000846 33.0 25.0 35.0 2.0 35.0 96-97 26.203447129551897 33.0 25.0 35.0 2.0 35.0 98-99 25.33527814244905 32.0 21.0 34.0 2.0 35.0 100-101 22.78459666105104 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26357844149633536 1101 2 0.20196619580121933 1101 3 0.12753605513905342 1101 4 0.1396248229680097 1101 5 0.12854781373781066 1101 6 0.22356060546294287 1101 7 0.1737747842922488 1101 8 0.21579885394859843 1101 9 0.27728368373391277 1101 10-11 0.25628002136839 1101 12-13 0.3062844320207816 1101 14-15 0.2649579670095008 1101 16-17 0.24702831512070844 1101 18-19 0.40005547156851406 1101 20-21 0.12173970998077976 1101 22-23 0.13277855375177694 1101 24-25 0.17788129657013485 1101 26-27 0.2589295109752001 1101 28-29 0.1729073594033821 1101 30-31 0.24672531437594358 1101 32-33 0.15345482500676155 1101 34-35 0.24927941767924722 1101 36-37 0.32120997877230906 1101 38-39 0.45145778448461726 1101 40-41 0.34376163314583863 1101 42-43 0.3937752468008071 1101 44-45 0.7575572486976299 1101 46-47 0.8300499098057941 1101 48-49 0.7519337304104212 1101 50-51 0.5910215558376066 1101 52-53 0.4483546529160307 1101 54-55 0.8496120156105533 1101 56-57 0.7378477968543535 1101 58-59 0.7696747843523823 1101 60-61 0.5599558531913402 1101 62-63 0.5549145242081224 1101 64-65 0.695634185807755 1101 66-67 0.5017984150786319 1101 68-69 0.935496651649462 1101 70-71 0.9137283815930566 1101 72-73 1.009188341481984 1101 74-75 1.0880325728413531 1101 76-77 0.8089452977739597 1101 78-79 1.059207556032856 1101 80-81 0.9356720196456223 1101 82-83 0.7631889971300971 1101 84-85 0.7487798848448577 1101 86-87 0.4369061229859774 1101 88-89 0.8006026899609893 1101 90-91 0.6920873687396707 1101 92-93 0.7429097153190973 1101 94-95 0.5261820187804709 1101 96-97 0.7726310746761129 1101 98-99 0.7073741886418858 1101 100-101 0.8345117692745845 1102 1 0.20803837752520593 1102 2 0.17086911824306128 1102 3 0.12779850080460164 1102 4 0.08485022552138588 1102 5 0.03779902344830077 1102 6 0.07442038839850795 1102 7 0.07176543466541574 1102 8 0.09215413976832565 1102 9 0.09898192589471932 1102 10-11 0.0827925012065478 1102 12-13 0.15112026678936274 1102 14-15 0.23668354726046914 1102 16-17 0.22270288249542602 1102 18-19 0.062892071803617 1102 20-21 0.12174381069430495 1102 22-23 0.2054814707259922 1102 24-25 0.19343256915823304 1102 26-27 0.08923515082320677 1102 28-29 0.16051500313339773 1102 30-31 0.1272059180009748 1102 32-33 0.08357729965067762 1102 34-35 -0.04425785780668434 1102 36-37 0.10810546525362241 1102 38-39 -0.053613431240250975 1102 40-41 0.10405989170949681 1102 42-43 0.21474492885882057 1102 44-45 -0.00983114354929171 1102 46-47 0.02148488615834765 1102 48-49 -0.005461222978134117 1102 50-51 -0.19366501295869654 1102 52-53 -0.18251415158929518 1102 54-55 -0.14337986497667288 1102 56-57 -0.11473701957037008 1102 58-59 -0.007213594225490283 1102 60-61 -0.13186048951895657 1102 62-63 -0.2400812383879085 1102 64-65 -0.04454597220641432 1102 66-67 -0.13607577503044155 1102 68-69 -0.38378763715012454 1102 70-71 -0.17683997730138756 1102 72-73 -0.1084501942899081 1102 74-75 0.02767006976581854 1102 76-77 0.12772246500105666 1102 78-79 0.21875401711708164 1102 80-81 0.18927517992884546 1102 82-83 0.015427986714286135 1102 84-85 -0.06928922584706854 1102 86-87 -0.01763883450329473 1102 88-89 -0.07574576133620425 1102 90-91 -0.28941841326640017 1102 92-93 -0.2806584522087192 1102 94-95 -0.2603485041971929 1102 96-97 -0.09367609610493588 1102 98-99 -0.1650471460036762 1102 100-101 0.12638552197422115 1103 1 0.24369420588176638 1103 2 0.10443470107871633 1103 3 0.2252548157791665 1103 4 0.10273343720021444 1103 5 0.06707352024104551 1103 6 0.05634916521779587 1103 7 0.18603218980808123 1103 8 0.14850229283180738 1103 9 0.18851963879009048 1103 10-11 0.12349146161354696 1103 12-13 0.2892091851702574 1103 14-15 0.25973154549094346 1103 16-17 0.3938873549572719 1103 18-19 0.363555641810251 1103 20-21 0.1540430807673019 1103 22-23 0.17803732120155047 1103 24-25 0.0837886850617906 1103 26-27 0.036304383293895626 1103 28-29 0.003635994064609349 1103 30-31 0.0478318857072324 1103 32-33 0.06900640825497106 1103 34-35 0.14575541359344868 1103 36-37 0.04191988684176806 1103 38-39 -0.06598348211395688 1103 40-41 0.19253589259425752 1103 42-43 0.2862335375737004 1103 44-45 0.4032245963312704 1103 46-47 0.5984700664281988 1103 48-49 0.27621871508866747 1103 50-51 0.1960607114385624 1103 52-53 0.547528980461145 1103 54-55 0.50683026552543 1103 56-57 0.34792610805626367 1103 58-59 0.7678361735250121 1103 60-61 0.8426252437259052 1103 62-63 0.6318552800814601 1103 64-65 0.7559848837988952 1103 66-67 0.6652900732332157 1103 68-69 0.799269208680446 1103 70-71 0.8251175542181848 1103 72-73 0.2040470408350643 1103 74-75 0.029384292282959024 1103 76-77 0.2139589171134233 1103 78-79 0.41372747431681844 1103 80-81 0.3983550295196423 1103 82-83 0.38870823444709046 1103 84-85 0.3540573788863952 1103 86-87 0.31354895580858155 1103 88-89 0.7459806266310629 1103 90-91 0.8939259795670473 1103 92-93 0.6717912270657784 1103 94-95 0.4471387773776776 1103 96-97 0.6144083984759092 1103 98-99 0.8014923364117195 1103 100-101 1.113180478842171 1104 1 0.09333084375369793 1104 2 0.04506955641945609 1104 3 -0.09961835027047528 1104 4 0.03478471925149762 1104 5 -0.14436895884849577 1104 6 -0.19079192898212227 1104 7 -0.013926674856662657 1104 8 0.018137559525904123 1104 9 0.01747891480819419 1104 10-11 0.05027056486323289 1104 12-13 -0.0075342674555471945 1104 14-15 -0.012598085662219205 1104 16-17 -0.047155425791387984 1104 18-19 -0.03554623670338941 1104 20-21 -0.09607004140465136 1104 22-23 -0.2110035398981367 1104 24-25 -0.11700659808642655 1104 26-27 -0.2389732802190636 1104 28-29 -0.2134900171790548 1104 30-31 -0.315283430349254 1104 32-33 -0.5001523741391054 1104 34-35 -0.3505629739014111 1104 36-37 -0.19605831143054786 1104 38-39 -0.15579220280502426 1104 40-41 -0.0035768182243387514 1104 42-43 -0.12249400938913624 1104 44-45 -0.34729302330399037 1104 46-47 -0.1401697251527665 1104 48-49 -0.2152354190125223 1104 50-51 -0.35845731955006244 1104 52-53 -0.376346653708616 1104 54-55 -0.6349906586484266 1104 56-57 -0.21721855120604516 1104 58-59 -0.2282559696259625 1104 60-61 -0.17279365110475453 1104 62-63 -0.34347380260056326 1104 64-65 -0.462663134849123 1104 66-67 -0.22521109226794778 1104 68-69 -0.08387901564424993 1104 70-71 -0.22449166009690913 1104 72-73 -0.4737467340175314 1104 74-75 -0.4379473956369644 1104 76-77 -0.4591008662404761 1104 78-79 -0.1007624475260478 1104 80-81 -0.027567087578944438 1104 82-83 -0.13491715976654106 1104 84-85 -0.08901817301268267 1104 86-87 -0.4032891080883054 1104 88-89 -0.11324397229941852 1104 90-91 0.10314029466371366 1104 92-93 -0.045722141918179915 1104 94-95 -0.023744261152419455 1104 96-97 -0.046533600701387456 1104 98-99 0.08146285608811965 1104 100-101 0.425617061866987 1105 1 0.034426774284234796 1105 2 -0.10587715534964559 1105 3 -5.174701872974197E-4 1105 4 0.006778744643582968 1105 5 -0.12126128411317438 1105 6 -0.10066760442033029 1105 7 0.15693539663362088 1105 8 -0.031035806609203576 1105 9 0.14903743814284098 1105 10-11 0.19086421830668598 1105 12-13 0.10696440729440582 1105 14-15 0.20595102180750757 1105 16-17 -0.0700946985878872 1105 18-19 -0.0920048171482648 1105 20-21 0.055418485298041276 1105 22-23 -0.1656887747090039 1105 24-25 0.05107318050163201 1105 26-27 -0.08484889708268639 1105 28-29 0.1805998069507524 1105 30-31 -0.016996004848813584 1105 32-33 -0.21589356232460943 1105 34-35 -0.03494479234947789 1105 36-37 -0.17747552357305807 1105 38-39 -0.1548110784658192 1105 40-41 -0.18235378083910803 1105 42-43 0.31019646784731236 1105 44-45 0.16099894172697304 1105 46-47 0.4529272597203402 1105 48-49 0.5742401919936455 1105 50-51 0.7802619470961076 1105 52-53 0.48509149884890235 1105 54-55 0.39984291630506874 1105 56-57 0.175066443449019 1105 58-59 0.28357609703192566 1105 60-61 0.534564049256943 1105 62-63 0.10759079052571252 1105 64-65 0.033478264204902075 1105 66-67 0.17197586317143276 1105 68-69 0.08548574295523181 1105 70-71 0.33316403847826237 1105 72-73 0.4520758728444818 1105 74-75 0.07270585744918634 1105 76-77 0.23797333311812707 1105 78-79 0.2777977891152865 1105 80-81 0.2825121345505366 1105 82-83 0.5016385080569741 1105 84-85 0.4133248104839282 1105 86-87 0.37858278928901967 1105 88-89 0.15346076784906515 1105 90-91 0.03562942175592454 1105 92-93 0.24292244212903213 1105 94-95 0.19185781238797617 1105 96-97 -0.004744293492318974 1105 98-99 -0.46175420668919287 1105 100-101 -0.7970725367506475 1106 1 -0.26057162842698744 1106 2 -0.17262993671553772 1106 3 -0.1947477853393167 1106 4 -0.14497757853654747 1106 5 -0.13238674767416114 1106 6 -0.18200432678376188 1106 7 -0.21946917577911762 1106 8 -0.18427830282432467 1106 9 -0.42263915949316555 1106 10-11 -0.19137393050507256 1106 12-13 -0.37406952467497945 1106 14-15 -0.24843596653923328 1106 16-17 -0.22034401453160513 1106 18-19 -0.4202198816648348 1106 20-21 -0.3849054170867774 1106 22-23 -0.3228828244610824 1106 24-25 -0.4564896346156715 1106 26-27 -0.21851418869206185 1106 28-29 -0.4611838784805826 1106 30-31 -0.38341405004513973 1106 32-33 -0.261221839891725 1106 34-35 -0.1833120854311332 1106 36-37 -0.46757094421558776 1106 38-39 -0.3579167634249174 1106 40-41 -0.3758410094736391 1106 42-43 -0.6355443269080254 1106 44-45 -0.6825570397722558 1106 46-47 -0.43419998169249396 1106 48-49 -0.8845011444509296 1106 50-51 -0.759434151352437 1106 52-53 -0.6994439679066531 1106 54-55 -0.36313331881583366 1106 56-57 -0.3727202698862513 1106 58-59 -0.4436347589759677 1106 60-61 -0.8249648200014974 1106 62-63 -0.4984900183968861 1106 64-65 -0.7039969083717352 1106 66-67 -0.39011700180905606 1106 68-69 -0.5056013856751278 1106 70-71 -0.41212395963276904 1106 72-73 -0.45819122161778836 1106 74-75 -0.7029931300086254 1106 76-77 -0.9306795274177269 1106 78-79 -0.7863993302091288 1106 80-81 -0.5852254100693806 1106 82-83 -0.697668407508953 1106 84-85 -0.8195737825130038 1106 86-87 -0.78398648443072 1106 88-89 -0.820767324988136 1106 90-91 -0.9995387102495741 1106 92-93 -0.4490925029381252 1106 94-95 -0.26357783076382546 1106 96-97 -0.4033442351565526 1106 98-99 0.02941209190658256 1106 100-101 0.03970538455162753 1107 1 -0.09702549108793335 1107 2 0.06390439804841463 1107 3 -0.2237982145238675 1107 4 -0.024349896133117 1107 5 -0.2325476918801641 1107 6 -0.16183265296402283 1107 7 -0.33612690110100374 1107 8 -0.2016871011075807 1107 9 -0.22398036120990383 1107 10-11 -0.09895172020464571 1107 12-13 -0.26713551179944517 1107 14-15 -0.0761586060242081 1107 16-17 -0.02656719049727485 1107 18-19 0.30370715696177086 1107 20-21 0.36654308076729336 1107 22-23 0.13021535150458163 1107 24-25 0.11948944263755124 1107 26-27 0.2124407469302625 1107 28-29 0.008370842549453528 1107 30-31 0.32018037055571824 1107 32-33 -0.2744595008359383 1107 34-35 -0.4312332227701887 1107 36-37 -0.2950119313400492 1107 38-39 0.2362892451587726 1107 40-41 -0.08587319831483597 1107 42-43 0.09911232545248794 1107 44-45 0.022163990270662737 1107 46-47 0.04553446036759823 1107 48-49 0.3929800787250386 1107 50-51 0.6277841962870383 1107 52-53 0.368930495612652 1107 54-55 0.09044768976785633 1107 56-57 0.16762307775322682 1107 58-59 0.012722537161366176 1107 60-61 0.6246328194834803 1107 62-63 0.6372530073541895 1107 64-65 0.4774810959201119 1107 66-67 0.3791158308089706 1107 68-69 0.591219966256201 1107 70-71 0.977106190581825 1107 72-73 0.9782894650774914 1107 74-75 0.807414595313265 1107 76-77 0.1010801292346315 1107 78-79 0.4177047470440911 1107 80-81 0.28310881739843197 1107 82-83 0.17270444656830009 1107 84-85 0.24790207585609636 1107 86-87 0.44129516792979473 1107 88-89 0.41359426299469604 1107 90-91 0.04278961593068331 1107 92-93 -0.511163318388764 1107 94-95 -0.47322107110717226 1107 96-97 -0.7443984197059095 1107 98-99 0.19789385156323291 1107 100-101 1.468956993993686 1108 1 -0.12486640017883843 1108 2 -0.14973196558795365 1108 3 0.004042694567040428 1108 4 0.18076374023051756 1108 5 0.06177048993801293 1108 6 0.22794007430870522 1108 7 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2316 58-59 0.48325119328785604 2316 60-61 0.3642326218550238 2316 62-63 0.7135746476703915 2316 64-65 0.47166340817307884 2316 66-67 0.40537571223189417 2316 68-69 0.4637619623036322 2316 70-71 0.3427064870245111 2316 72-73 0.30160962318025497 2316 74-75 0.7503987850365839 2316 76-77 0.6528587853611185 2316 78-79 0.6976207549492273 2316 80-81 0.8532298648292596 2316 82-83 0.8002365611928077 2316 84-85 0.7848215422592588 2316 86-87 1.3926662153606237 2316 88-89 1.159715804496674 2316 90-91 1.3921473234405646 2316 92-93 1.3414552586863309 2316 94-95 1.48442467988097 2316 96-97 1.5373332996616789 2316 98-99 1.6390746816027573 2316 100-101 2.4103478042703657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 13.0 6 18.0 7 32.0 8 112.0 9 234.0 10 384.0 11 638.0 12 977.0 13 1410.0 14 1800.0 15 2702.0 16 3886.0 17 5909.0 18 5299.0 19 2902.0 20 2248.0 21 2244.0 22 2164.0 23 2297.0 24 2706.0 25 3134.0 26 3713.0 27 4305.0 28 5507.0 29 6603.0 30 8236.0 31 10125.0 32 12947.0 33 17225.0 34 23871.0 35 34941.0 36 51177.0 37 75592.0 38 55199.0 39 4405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39769951054015 19.990081182142745 15.408545399725543 20.20367390759156 2 16.000867698514206 21.281432942117746 40.18120249491495 22.536496864453095 3 19.369115567300163 26.267600475543862 31.17263256348567 23.190651393670308 4 12.710938072244351 17.69428840270226 37.24426840056119 32.3505051244922 5 12.782817224481285 39.14790611693959 33.434497995023484 14.634778663555641 6 30.59285219262907 37.40118660589021 16.913078019619 15.09288318186172 7 27.582670821101978 35.50858965185006 19.710278846749794 17.198460680298172 8 27.253621514415627 34.741183563311004 18.93301254782202 19.072182374451348 9 27.61168801167449 16.47190403479809 20.441343016999003 35.47506493652842 10-11 25.12395693060102 27.84607929862915 26.0153199497411 21.01464382102873 12-13 26.430528458433994 25.738172546644222 27.456454827826647 20.374844167095134 14-15 23.160225601613693 26.243231669868887 27.167837684033785 23.428705044483635 16-17 20.932804074802373 30.01814278711524 27.617885858204538 21.431167279877847 18-19 21.015066401473963 26.79822065460528 32.058220316540925 20.12849262737983 20-21 21.923473498571678 25.938832889154334 32.3683943633403 19.769299248933688 22-23 21.664032544787116 26.074707640643798 32.345044611037395 19.916215203531696 24-25 21.40623503360923 26.157729559783867 32.4755889362805 19.960446470326403 26-27 21.022768890967384 26.55553614793863 32.53075011691524 19.890944844178748 28-29 20.698646036995928 26.65301246908085 32.623578000777556 20.024763493145667 30-31 20.809410584794993 26.596790642378622 32.45826313802604 20.135535634800345 32-33 20.62868701438464 26.70243575368631 32.349237383156506 20.319639848772546 34-35 21.16057493478175 26.559885283495134 32.36177393636502 19.917765845358097 36-37 21.330029693319286 26.32662087772776 32.007792383410056 20.335557045542902 38-39 21.104542323604107 26.799385285446448 32.0606600997573 20.03541229119214 40-41 21.142181817157375 26.689833531007633 32.00971374319996 20.158270908635036 42-43 20.902307967574053 26.870169427530495 31.69898084254482 20.528541762350628 44-45 21.16564590012452 27.18276885976527 31.303914221804025 20.347671018306183 46-47 21.454972644959177 27.62901380992895 30.426918937801794 20.48909460731008 48-49 21.57822527481815 27.730573976932742 30.05180836258529 20.639392385663818 50-51 21.721761766048196 28.04300178610668 29.534992478068073 20.700243969777045 52-53 21.893752007257113 28.328525306934267 29.377510832145415 20.400211853663208 54-55 21.67992630197035 28.262039316884625 29.41737425414551 20.64066012699951 56-57 21.652176852733533 28.60742840078656 29.073534631876086 20.666860114603818 58-59 21.767963894726762 28.002293203216116 29.134949656582958 21.094793245474165 60-61 21.76669615339107 27.94341366118063 29.589082775057612 20.700807410370686 62-63 21.514274767439893 28.055960919760427 29.923203047086734 20.50656126571295 64-65 21.438914588040408 28.000039440841558 29.63669350522028 20.92435246589776 66-67 21.437365126407897 28.224711377555906 29.313560324767156 21.024363171269037 68-69 21.529658850326022 27.999233719713263 29.400371589594926 21.07073584036579 70-71 21.509485522393945 28.262321037181444 29.2303119770567 20.99788146336791 72-73 21.648655349023276 28.073286718014884 28.937041148066555 21.341016784895285 74-75 21.598931716634457 28.393180115054566 28.7906874538683 21.217200714442672 76-77 21.505823158535282 28.228937182008213 28.985074458674447 21.280165200782054 78-79 21.520613474118356 28.31472101239006 28.812239056575066 21.352426456916515 80-81 21.492864024881538 28.57601658769108 28.65081332649692 21.280306060930464 82-83 21.533886737595946 28.37011504291395 28.62296435176903 21.473033867721075 84-85 21.417121414022464 28.125360957180973 28.728951794432593 21.72856583436397 86-87 21.367216772499592 28.185411396149444 28.70152297992461 21.74584885142635 88-89 21.363695268789336 27.897493252798895 28.644897200263692 22.093914278148084 90-91 21.202643991006372 28.20679706298356 28.7570650122 21.833493933810065 92-93 21.160363883262104 28.130520159234145 28.745678956695187 21.963437000808568 94-95 21.03027929750227 27.978346977986373 28.632219786906767 22.35915393760459 96-97 21.0911308816155 28.057087800947706 28.547140257266975 22.30464106016982 98-99 20.97163608088844 28.137554302199135 28.324477825545557 22.566331791366867 100-101 20.965330876188947 28.172907447091795 28.335636906195976 22.526124770523285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 383.0 1 260.5 2 171.0 3 222.0 4 193.0 5 178.0 6 337.0 7 724.0 8 1154.5 9 1318.5 10 1108.5 11 920.0 12 944.0 13 967.0 14 972.0 15 1004.0 16 1057.0 17 1114.5 18 1171.5 19 1205.5 20 1269.0 21 1383.0 22 1541.5 23 1798.5 24 2020.5 25 2331.5 26 2903.5 27 3379.0 28 3686.5 29 4164.5 30 4804.5 31 5460.5 32 6193.5 33 6924.5 34 7677.5 35 8322.0 36 9263.5 37 10085.0 38 10358.5 39 10375.5 40 10268.0 41 10636.0 42 11083.5 43 11741.0 44 12492.5 45 12996.5 46 12844.0 47 12663.5 48 13671.5 49 13415.0 50 12301.5 51 11528.0 52 10657.0 53 10218.5 54 9889.5 55 9190.5 56 8248.5 57 7476.0 58 6713.5 59 5980.0 60 5051.0 61 4032.0 62 3241.5 63 2519.0 64 1828.0 65 1369.5 66 1025.0 67 795.0 68 581.5 69 359.0 70 263.0 71 205.5 72 146.0 73 101.5 74 74.5 75 53.5 76 36.0 77 29.5 78 22.0 79 17.0 80 14.0 81 11.0 82 6.0 83 3.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022819344042460884 2 0.0 3 0.0 4 0.0 5 0.02676342819794795 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004084944303897319 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014086014841025237 24-25 0.0 26-27 0.0011268811872820188 28-29 0.0011268811872820188 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4086014841025235E-4 40-41 2.817202968205047E-4 42-43 0.01718493810605079 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.4086014841025235E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0021129022261537856 84-85 8.451608904615142E-4 86-87 0.0 88-89 0.0 90-91 0.012395693060102207 92-93 0.0036623638586665615 94-95 0.0 96-97 0.0 98-99 0.0011268811872820188 100-101 0.02211504330040962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 354962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.02925185320846 #Duplication Level Percentage of deduplicated Percentage of total 1 82.65591272511783 52.0974034030827 2 10.079224705089645 12.70571984844352 3 2.9869635531476124 5.647982342030857 4 1.3025903867012398 3.284051901798426 5 0.7144975864621279 2.2517124162815527 6 0.45953388063135464 1.7378446018437532 7 0.3112059790833528 1.3730556023708278 8 0.22372742101272464 1.128109757238386 9 0.17444227620738473 0.9895469544829987 >10 1.0108636285876273 11.259522910828878 >50 0.05775626340737476 2.487279419701994 >100 0.021042979691029768 2.098694922203757 >500 8.954459442991389E-4 0.3820939793819937 >1k 0.0013431689164487084 2.5569819403103726 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3731 1.0510984274373032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.8843200117195643 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2191 0.6172491703337258 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 730 0.20565581667896846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.17579346521599495 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 470 0.13240853950563722 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.817202968205047E-4 0.0 0.0 0.0 0.0 8 2.817202968205047E-4 0.0 0.0 0.0 0.0 9 2.817202968205047E-4 0.0 0.0 0.0 0.0 10-11 2.817202968205047E-4 0.0 0.0 0.0 0.0 12-13 2.817202968205047E-4 0.0 0.0 0.0 0.0 14-15 2.817202968205047E-4 0.0 0.0 0.0 0.0 16-17 2.817202968205047E-4 0.0 0.0 0.0 0.0 18-19 2.817202968205047E-4 0.0 0.0 0.0 0.0 20-21 2.817202968205047E-4 0.0 0.0 0.0 0.0 22-23 2.817202968205047E-4 0.0 0.0 4.225804452307571E-4 0.0 24-25 2.817202968205047E-4 0.0 0.0 5.634405936410094E-4 0.0 26-27 2.817202968205047E-4 0.0 0.0 0.0011268811872820188 0.0 28-29 2.817202968205047E-4 0.0 0.0 0.003098923265025552 0.0 30-31 2.817202968205047E-4 0.0 0.0 0.005775266084820347 0.0 32-33 2.817202968205047E-4 0.0 0.0 0.010001070537127918 0.0 34-35 4.225804452307571E-4 0.0 0.0 0.011973112614871452 0.0 36-37 5.634405936410094E-4 0.0 0.0 0.014367735137845741 0.0 38-39 5.634405936410094E-4 0.0 0.0 0.023101064339281385 0.0 40-41 5.634405936410094E-4 0.0 0.0 0.03887740096122966 0.0 42-43 5.634405936410094E-4 0.0 0.0 0.0600064232227675 0.0 44-45 5.634405936410094E-4 0.0 0.0 0.08648813112389495 0.0 46-47 5.634405936410094E-4 0.0 0.0 0.14057842811343185 0.0 48-49 8.451608904615142E-4 0.0 0.0 0.19819022881322507 0.0 50-51 8.451608904615142E-4 0.0 0.0 0.2415751545235828 0.0 52-53 8.451608904615142E-4 0.0 0.0 0.2979192138876837 0.0 54-55 8.451608904615142E-4 0.0 0.0 0.35468585369701544 0.0 56-57 8.451608904615142E-4 0.0 0.0 0.46652881153475584 0.0 58-59 8.451608904615142E-4 0.0 0.0 0.5937255255492138 0.0 60-61 8.451608904615142E-4 0.0 0.0 0.7299372890619278 0.0 62-63 8.451608904615142E-4 0.0 0.0 0.8706565773237699 0.0 64-65 8.451608904615142E-4 0.0 0.0 1.0279973630980217 0.0 66-67 8.451608904615142E-4 0.0 0.0 1.2419639285331951 0.0 68-69 8.451608904615142E-4 0.0 0.0 1.517627238972059 0.0 70-71 8.451608904615142E-4 0.0 0.0 1.7341292870786167 0.0 72-73 8.451608904615142E-4 0.0 0.0 1.9557023005279437 0.0 74-75 8.451608904615142E-4 0.0 0.0 2.2084054067759364 0.0 76-77 8.451608904615142E-4 0.0 0.0 2.4839278570663903 0.0 78-79 8.451608904615142E-4 0.0 0.0 2.8056524360354063 0.0 80-81 0.0011268811872820188 0.0 0.0 3.2007651523261647 0.0 82-83 0.0011268811872820188 0.0 0.0 3.6137671074650246 0.0 84-85 0.0014086014841025235 0.0 0.0 4.043390560116294 0.0 86-87 0.0016903217809230283 0.0 0.0 4.483860244195153 0.0 88-89 0.0016903217809230283 0.0 0.0 5.112800806846931 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACGA 25 0.0050107404 57.022068 5 GGTATCA 1850 0.0 44.436115 1 GTATCAA 3090 0.0 36.599926 1 CTGTCCG 85 1.6294363E-5 33.528217 9 CCGCTCC 65 0.005839718 29.229729 9 TATCAAC 3950 0.0 28.150133 2 ATCAACG 4015 0.0 27.694407 3 TCAACGC 4030 0.0 27.591322 4 CAACGCA 4065 0.0 27.236864 5 GGGCGTA 70 0.008385351 27.141891 9 AACGCAG 4155 0.0 26.635635 6 GTACATG 2455 0.0 25.162487 1 TACATGG 2525 0.0 23.90034 2 ATATAGG 120 1.7030956E-4 23.759193 4 GTACACT 160 1.8541032E-6 23.749155 6 ACGCAGA 4690 0.0 23.495964 7 ACATGGG 2495 0.0 23.235445 3 CGCAGAG 4750 0.0 22.999182 8 TAAGGCA 145 2.3215069E-5 22.939913 4 GAGTACT 2755 0.0 22.844013 12-13 >>END_MODULE