FastQCFastQC Report
Thu 26 May 2016
SRR937741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303770
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31491.036639562827139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.8078480429272147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.5583171478421174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.19718866247489877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4460.14682160845376435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.1402376798235507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.055.3117681
GTATCAA20750.041.9442561
TATCAAC26800.032.109962
GTAAGTA600.00392853831.6960162
ATCAACG27350.031.6380753
TGGTATC5000.031.379062
TCAACGC28150.030.7440264
GTACATG20900.030.7204071
GTGGTAT5200.030.1821421
CAACGCA28950.029.8895075
CATATAC1601.875378E-929.7150173
AACGCAG29600.029.3840666
TACATGG21400.029.3262232
CATGGGA12800.028.6054294
ACATGGG21050.028.4586583
GTATATG1056.830611E-527.1769961
AGAGCGA700.00834418527.1680165
CGGTGTA350.00841141427.12768280-81
ACGCAGA32750.026.4126857
ATGGGAT4550.026.123095
Produced by FastQC (version 0.11.5)