##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937741_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303770 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81945550910228 33.0 31.0 34.0 30.0 34.0 2 31.94759851203213 33.0 31.0 34.0 30.0 34.0 3 31.930766698488988 34.0 31.0 34.0 30.0 34.0 4 35.538887974454354 37.0 35.0 37.0 33.0 37.0 5 35.53077328241762 37.0 35.0 37.0 33.0 37.0 6 35.60447048753991 37.0 35.0 37.0 33.0 37.0 7 35.54376666556934 37.0 35.0 37.0 33.0 37.0 8 35.5419922968035 37.0 35.0 37.0 33.0 37.0 9 37.17452019620107 39.0 37.0 39.0 34.0 39.0 10-11 37.071751654212065 39.0 37.0 39.0 33.0 39.0 12-13 36.95093327188333 39.0 37.0 39.0 33.0 39.0 14-15 38.079109194456336 40.0 38.0 41.0 33.0 41.0 16-17 38.033311386904565 40.0 38.0 41.0 33.0 41.0 18-19 38.03861144945189 40.0 38.0 41.0 33.0 41.0 20-21 37.992978240115875 40.0 38.0 41.0 33.0 41.0 22-23 37.81217862198373 40.0 38.0 41.0 33.0 41.0 24-25 37.65294466207986 40.0 37.0 41.0 32.5 41.0 26-27 37.57947954044178 40.0 37.0 41.0 32.5 41.0 28-29 37.55658886657669 40.0 37.0 41.0 32.0 41.0 30-31 37.326561214076435 39.5 37.0 41.0 32.0 41.0 32-33 37.013834480034234 39.0 36.0 41.0 31.5 41.0 34-35 37.018462981861276 39.5 36.0 41.0 31.0 41.0 36-37 36.893498370477666 40.0 36.0 41.0 31.0 41.0 38-39 36.73704282845574 39.5 35.0 41.0 31.0 41.0 40-41 36.464165322447904 39.0 35.0 41.0 30.0 41.0 42-43 36.156811074167955 39.0 35.0 41.0 30.0 41.0 44-45 35.8261398426441 39.0 35.0 41.0 28.5 41.0 46-47 35.31517430951048 39.0 35.0 41.0 26.0 41.0 48-49 34.82993876946374 39.0 35.0 40.5 23.5 41.0 50-51 33.846199427198215 38.0 34.0 39.5 22.0 40.5 52-53 33.906891727293676 38.0 33.5 39.5 21.5 40.5 54-55 34.24022122000197 38.0 34.0 40.0 20.5 41.0 56-57 34.02907792079534 38.0 34.0 40.0 19.5 41.0 58-59 33.79045824143266 38.0 34.0 40.0 17.5 41.0 60-61 33.47907627481318 38.0 33.5 40.0 14.0 41.0 62-63 33.115641768443226 37.0 33.0 40.0 10.0 41.0 64-65 32.730987260098104 37.0 33.0 40.0 7.5 41.0 66-67 32.323856865391576 36.0 32.0 39.0 6.0 41.0 68-69 31.8466767620239 36.0 31.5 39.0 2.0 40.5 70-71 31.42485597656121 35.0 31.0 38.5 2.0 40.0 72-73 30.983729466372587 35.0 31.0 38.0 2.0 40.0 74-75 30.534285808341835 35.0 30.5 37.0 2.0 39.0 76-77 30.047037232116402 35.0 30.0 36.5 2.0 39.0 78-79 29.531365835994336 34.0 29.0 36.0 2.0 39.0 80-81 29.007471113013136 34.0 29.0 36.0 2.0 37.5 82-83 28.5160499720183 34.0 28.5 35.0 2.0 37.0 84-85 28.08714487934951 34.0 27.5 35.0 2.0 36.5 86-87 27.64743885176285 34.0 26.5 35.0 2.0 36.0 88-89 27.202943016097706 33.5 26.0 35.0 2.0 36.0 90-91 26.733245547618264 33.0 25.0 35.0 2.0 35.0 92-93 26.21257365770155 33.0 24.5 35.0 2.0 35.0 94-95 25.614524146558253 32.0 21.5 35.0 2.0 35.0 96-97 24.927038548902132 32.0 18.0 34.0 2.0 35.0 98-99 24.04312967047437 31.5 2.0 34.0 2.0 35.0 100-101 21.817103400599137 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09969691927927116 1101 2 0.19510108866658982 1101 3 0.33566029647982987 1101 4 0.14683020188702045 1101 5 0.14215142039554252 1101 6 0.25070086918567824 1101 7 0.27451012003569986 1101 8 -0.03697083765289477 1101 9 0.13315422954976697 1101 10-11 0.20959665264633287 1101 12-13 0.19157365553846972 1101 14-15 0.34306634064716235 1101 16-17 0.4650681994289627 1101 18-19 0.5492868742548964 1101 20-21 0.40636009207587165 1101 22-23 0.4584390023742131 1101 24-25 0.3962649574323507 1101 26-27 0.24621444953464788 1101 28-29 0.31243659521584277 1101 30-31 0.37515262384295767 1101 32-33 0.5350563313672154 1101 34-35 0.7958110263963221 1101 36-37 0.48502707235527254 1101 38-39 0.510701855225129 1101 40-41 0.6736474427507346 1101 42-43 0.8551232203010386 1101 44-45 0.9277072001445461 1101 46-47 1.0410163587556056 1101 48-49 1.2263150225049344 1101 50-51 1.1384990324355826 1101 52-53 1.2128933703731093 1101 54-55 1.447362737079871 1101 56-57 1.0639006806706064 1101 58-59 1.276876429724652 1101 60-61 1.2975529002336117 1101 62-63 1.5130727384752873 1101 64-65 1.4547072332521722 1101 66-67 1.5914531374181458 1101 68-69 1.5893128135555763 1101 70-71 1.6456741992708501 1101 72-73 1.6433128887352346 1101 74-75 1.7017189234426802 1101 76-77 1.743016799451702 1101 78-79 1.895702306850655 1101 80-81 1.9295638443510654 1101 82-83 1.884629131619704 1101 84-85 1.9716354689793185 1101 86-87 2.1822879901444985 1101 88-89 2.193583231481046 1101 90-91 2.4381722403539285 1101 92-93 2.24014184890871 1101 94-95 2.3572203349456906 1101 96-97 2.436373790354697 1101 98-99 2.612145820127868 1101 100-101 2.5230793593325878 1102 1 -0.021204054746700507 1102 2 0.0658397250302265 1102 3 0.13150039388242618 1102 4 0.1209170525363703 1102 5 0.2658933035124278 1102 6 0.13197116139346576 1102 7 0.1413323602954364 1102 8 -0.06016483115939053 1102 9 0.10391315812119473 1102 10-11 0.06374640913984564 1102 12-13 0.31622868800599946 1102 14-15 0.3255643114263833 1102 16-17 0.29407591046791737 1102 18-19 0.2951675560730749 1102 20-21 0.28572291675119743 1102 22-23 0.24121091795861815 1102 24-25 0.2395786749648181 1102 26-27 0.037600407326848995 1102 28-29 -0.15579595348545894 1102 30-31 0.1777906108559435 1102 32-33 0.3860709417568273 1102 34-35 0.30478018224047787 1102 36-37 0.2503071048228023 1102 38-39 0.44279398184851004 1102 40-41 0.32442869275073605 1102 42-43 0.19398279822311082 1102 44-45 0.04791823910558435 1102 46-47 0.28129639122314387 1102 48-49 0.29960033094649674 1102 50-51 0.3495379934745486 1102 52-53 0.1822825749185597 1102 54-55 0.444207298768184 1102 56-57 0.7807229209303514 1102 58-59 0.4561073550493262 1102 60-61 0.6698844749089403 1102 62-63 0.939614946267497 1102 64-65 0.8423898631223068 1102 66-67 0.7631759458597038 1102 68-69 0.7550392745945338 1102 70-71 0.7067537447253969 1102 72-73 0.9360787166573097 1102 74-75 0.8864693292868395 1102 76-77 1.0524932604906638 1102 78-79 1.0284640763311792 1102 80-81 0.988533000195222 1102 82-83 0.6416299433080148 1102 84-85 0.8845210209273695 1102 86-87 0.9667340128717719 1102 88-89 0.8620795788836411 1102 90-91 0.8161957793149668 1102 92-93 0.998928374882734 1102 94-95 1.0500064550755575 1102 96-97 1.6007000890559944 1102 98-99 1.4115269077902042 1102 100-101 2.085203953488435 1103 1 0.16317094525329878 1103 2 0.04525782847850479 1103 3 0.012373238710011947 1103 4 0.17420369046740092 1103 5 0.12225106213311676 1103 6 -0.013987890330668051 1103 7 0.1419250327092314 1103 8 0.016936461944055736 1103 9 0.20870841674188512 1103 10-11 0.2057453315536364 1103 12-13 0.1628342914542671 1103 14-15 0.1571645269436246 1103 16-17 0.34119336736447536 1103 18-19 0.03261367676272897 1103 20-21 0.18275955468222804 1103 22-23 0.278333762786211 1103 24-25 0.40884052841308716 1103 26-27 0.273403209050997 1103 28-29 0.14089046892833323 1103 30-31 0.2061311280973257 1103 32-33 0.33898042451544086 1103 34-35 0.11437069948186007 1103 36-37 0.22266701861590832 1103 38-39 0.6177131628829926 1103 40-41 0.857699166888672 1103 42-43 0.9972694361541485 1103 44-45 0.8248578942779972 1103 46-47 0.8676649257059026 1103 48-49 0.8470410637051131 1103 50-51 0.8075121314055806 1103 52-53 0.96399054905649 1103 54-55 0.9471976004923164 1103 56-57 0.908470765757933 1103 58-59 1.1662097257389803 1103 60-61 1.128478224908939 1103 62-63 1.1585804635088763 1103 64-65 1.450094604501615 1103 66-67 1.4537740062045295 1103 68-69 1.3949638435600527 1103 70-71 1.552335641277125 1103 72-73 1.5949418632090335 1103 74-75 1.3853648034247676 1103 76-77 1.6221592087665222 1103 78-79 2.0988035159863507 1103 80-81 1.6104080001952212 1103 82-83 1.6348950295149116 1103 84-85 1.6429800726515111 1103 86-87 1.6099721594234957 1103 88-89 1.76253755302157 1103 90-91 1.5881246586253148 1103 92-93 1.848658978331013 1103 94-95 1.8452381361100443 1103 96-97 1.6219285373318542 1103 98-99 1.2974374681350298 1103 100-101 2.0963300310746398 1104 1 0.21605556063791553 1104 2 0.3013365199020228 1104 3 0.30613981695934456 1104 4 0.3596590397158579 1104 5 0.12142214966627307 1104 6 0.2473958408806496 1104 7 0.1429749885005691 1104 8 0.2259329252508664 1104 9 0.3681359145314502 1104 10-11 0.5449363129859961 1104 12-13 0.4381437521085587 1104 14-15 0.5715378691186928 1104 16-17 0.648222545083307 1104 18-19 0.8727717227397491 1104 20-21 0.7335995193152982 1104 22-23 0.6842798282150326 1104 24-25 0.8692461429135321 1104 26-27 0.7668271701473657 1104 28-29 0.48328657856582424 1104 30-31 0.2557553544456823 1104 32-33 0.33903568534656614 1104 34-35 0.6327449258302238 1104 36-37 0.8239048612330606 1104 38-39 0.8911713856946619 1104 40-41 0.7944807760840646 1104 42-43 1.014455554633379 1104 44-45 1.0070528544901975 1104 46-47 1.0189967117359586 1104 48-49 0.7757822219721362 1104 50-51 1.0835123524489063 1104 52-53 1.0722465172262545 1104 54-55 0.7587305359476701 1104 56-57 1.155762985032915 1104 58-59 1.0672652076134312 1104 60-61 1.1292795069602235 1104 62-63 1.0920187924213423 1104 64-65 0.9621414656864076 1104 66-67 0.8438049522699593 1104 68-69 0.792979979722741 1104 70-71 0.6263851549818042 1104 72-73 0.9173888128111578 1104 74-75 1.0857682074919666 1104 76-77 0.9604660169009165 1104 78-79 1.072554621202972 1104 80-81 0.7882926155798344 1104 82-83 1.0777276997182703 1104 84-85 0.7085194183632701 1104 86-87 0.8627075705640799 1104 88-89 1.0438704442682543 1104 90-91 1.0732871254688163 1104 92-93 1.1808594646263266 1104 94-95 0.774145076870429 1104 96-97 1.0251391916200951 1104 98-99 1.2748081577902042 1104 100-101 1.8172352034884334 1105 1 -0.07781164181137612 1105 2 -0.06256512571604134 1105 3 -0.12564357377926783 1105 4 -0.27366472607059933 1105 5 -0.18617448255722735 1105 6 -0.4028624828851406 1105 7 -0.037468945674710596 1105 8 -0.15734300280117708 1105 9 0.06073063324557637 1105 10-11 -0.10331282593478619 1105 12-13 -0.23168020502883024 1105 14-15 -0.4961046873298329 1105 16-17 -0.6875744315718819 1105 18-19 -0.26466608820553006 1105 20-21 0.05392098889050345 1105 22-23 -0.5369218556732207 1105 24-25 -0.23023242578145187 1105 26-27 -0.2770878700363255 1105 28-29 -0.7947224149282448 1105 30-31 -0.6756453344176805 1105 32-33 -0.12843465525810416 1105 34-35 -0.7262055142769341 1105 36-37 -0.8989233056249546 1105 38-39 -0.9231225293455267 1105 40-41 -0.8084421094880696 1105 42-43 -0.8918303236674276 1105 44-45 -0.6997496713421754 1105 46-47 -0.23746138240870351 1105 48-49 0.06510483965296743 1105 50-51 0.5721748093949444 1105 52-53 0.803757232878759 1105 54-55 0.7737276656836016 1105 56-57 0.5260509681940277 1105 58-59 0.4482210055468414 1105 60-61 -0.1944384417577325 1105 62-63 0.1270838517401316 1105 64-65 0.19477636809742904 1105 66-67 0.2668101187452727 1105 68-69 0.04836172484329282 1105 70-71 -0.2844931458218696 1105 72-73 -0.013342147770547541 1105 74-75 -0.26880507991714 1105 76-77 -0.1578845380168019 1105 78-79 -0.10446891496235722 1105 80-81 0.44063188079223536 1105 82-83 0.3318895826114989 1105 84-85 -0.28313289698307287 1105 86-87 0.32740332257326443 1105 88-89 -0.15204899698700558 1105 90-91 -0.03903587491388549 1105 92-93 0.3977079146837319 1105 94-95 0.1305840359213306 1105 96-97 0.18905518855848058 1105 98-99 1.3202061677404515 1105 100-101 1.5025585865730129 1106 1 0.27061476547801533 1106 2 0.4404534890751677 1106 3 0.38587188264646954 1106 4 0.1379289907386152 1106 5 0.07021212373714292 1106 6 0.027178324314817814 1106 7 0.014769860295437809 1106 8 0.14929794973948418 1106 9 0.1707144221661352 1106 10-11 0.05260701307242499 1106 12-13 0.2672996149722877 1106 14-15 0.10373320187581925 1106 16-17 0.05644423911960672 1106 18-19 0.4743634830393688 1106 20-21 0.08609159652647946 1106 22-23 0.3358211707676091 1106 24-25 0.08739293058279429 1106 26-27 0.20906283710213813 1106 28-29 0.5109263077504949 1106 30-31 0.7762105546761688 1106 32-33 0.6797156046781652 1106 34-35 0.7353630474090167 1106 36-37 0.7219889868452753 1106 38-39 0.9114825352192923 1106 40-41 1.1753328360091686 1106 42-43 0.6948430510321018 1106 44-45 0.9043086885437859 1106 46-47 0.8908996215602158 1106 48-49 1.2499251202723372 1106 50-51 1.2287514766206158 1106 52-53 1.4944051170533967 1106 54-55 1.0489386892176142 1106 56-57 1.1794413198067488 1106 58-59 1.0538864983077545 1106 60-61 1.4160484496280432 1106 62-63 1.7978312384023312 1106 64-65 1.5716560148077008 1106 66-67 1.2566362688934127 1106 68-69 1.5604641341450964 1106 70-71 1.6697804301186565 1106 72-73 1.2678641801404567 1106 74-75 1.2665518433317828 1106 76-77 1.1478759852097653 1106 78-79 1.3921491184660084 1106 80-81 0.8758574383974675 1106 82-83 1.1036208140945298 1106 84-85 1.0533588018262492 1106 86-87 0.9314197572537957 1106 88-89 1.0443566154004955 1106 90-91 1.0333130545958653 1106 92-93 1.196452953534422 1106 94-95 0.8784738005811796 1106 96-97 1.1142885722020601 1106 98-99 0.41947107913851767 1106 100-101 0.9464486866345005 1107 1 -0.07634247756549328 1107 2 0.09119626865438946 1107 3 -0.01458811282898509 1107 4 0.01425807602630158 1107 5 -0.32708068977616733 1107 6 0.13418382917870275 1107 7 0.01812556499342577 1107 8 -0.09423572042113193 1107 9 0.07649075879233891 1107 10-11 -0.2523662166990803 1107 12-13 -0.1779722348127919 1107 14-15 0.5239126755203429 1107 16-17 0.6197313215417424 1107 18-19 -0.06990165533631654 1107 20-21 0.3189548798384436 1107 22-23 0.1465066394351382 1107 24-25 0.4369517873809272 1107 26-27 -0.08659736951878472 1107 28-29 0.15467489382997712 1107 30-31 0.010487758506954492 1107 32-33 0.0048419149111822435 1107 34-35 -0.06680119359844383 1107 36-37 0.018836886701997457 1107 38-39 0.27256013117736444 1107 40-41 0.5119024763077888 1107 42-43 0.03237415728352033 1107 44-45 0.7549232726626371 1107 46-47 0.8587187905519968 1107 48-49 0.8360988628256862 1107 50-51 1.4216856444812649 1107 52-53 1.1110629859923833 1107 54-55 1.2258295722581138 1107 56-57 1.3504691457625597 1107 58-59 1.2897849976667786 1107 60-61 0.9520939967210182 1107 62-63 1.236636758348034 1107 64-65 1.1037048799008247 1107 66-67 1.0693577831080212 1107 68-69 1.516123586675075 1107 70-71 1.054215993047542 1107 72-73 0.7557987250465708 1107 74-75 0.9407845557969061 1107 76-77 0.4876128074705264 1107 78-79 0.5926996048546691 1107 80-81 0.5390992753630073 1107 82-83 0.38255905404626844 1107 84-85 0.6353284873703267 1107 86-87 0.07011593401271199 1107 88-89 0.046354956400417535 1107 90-91 0.03921381622772202 1107 92-93 0.2832299688424662 1107 94-95 0.3276857755453584 1107 96-97 0.11930327697545451 1107 98-99 -0.056567681135970105 1107 100-101 0.46320835785084924 1108 1 0.40344872303107593 1108 2 0.05739065095615459 1108 3 0.05898881980835213 1108 4 0.16414621920303318 1108 5 0.5030460812902007 1108 6 0.27253829102309624 1108 7 0.1888439343695154 1108 8 -0.16751436819642862 1108 9 0.3135775099730438 1108 10-11 0.37534941839910374 1108 12-13 0.38330044726525614 1108 14-15 0.27310482068563857 1108 16-17 0.38351456787533067 1108 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0.8218276473896502 2316 84-85 0.9143998474579824 2316 86-87 0.584607099606469 2316 88-89 0.4491490941897638 2316 90-91 0.2679496058455797 2316 92-93 0.15080975243375505 2316 94-95 0.5487787765041325 2316 96-97 1.2544628441580308 2316 98-99 1.7936857088106137 2316 100-101 1.4141739789986367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1333.0 3 237.0 4 219.0 5 241.0 6 250.0 7 341.0 8 387.0 9 536.0 10 656.0 11 857.0 12 1263.0 13 1710.0 14 2022.0 15 2579.0 16 3416.0 17 4959.0 18 4127.0 19 2521.0 20 2365.0 21 2228.0 22 2427.0 23 2665.0 24 2791.0 25 3152.0 26 3715.0 27 4179.0 28 4749.0 29 5865.0 30 7154.0 31 8823.0 32 10978.0 33 14547.0 34 19705.0 35 28934.0 36 41324.0 37 57716.0 38 46749.0 39 6049.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2959360980992 18.915874389687957 15.270519368116439 21.517670144096407 2 15.063593398572575 20.085671916851343 41.37286439463246 23.477870289943624 3 18.5703483323042 25.62614952302018 31.190088593759803 24.613413550915823 4 11.887506974686428 16.675195869004657 37.920833594712114 33.5164635615968 5 12.45604941613816 38.0033476726412 34.44405193844772 15.096550972772924 6 29.872803761445187 36.870411614286894 17.849377216860514 15.407407407407408 7 26.90603524214541 34.62288397013557 20.67811931917533 17.792961468543698 8 26.44276300375849 34.10494081776094 19.926612043676393 19.525684134804173 9 26.706461554706422 16.129138701353554 20.884066865971082 36.28033287796894 10-11 24.25942760415034 27.19581468325527 27.014827121406853 21.529930591187536 12-13 25.72866228377698 25.088551419214873 28.04147522457498 21.141311072433165 14-15 22.606562434524957 25.5660755559295 27.629436423837785 24.197925585707758 16-17 20.544871741995937 29.329996486082084 27.96996177583183 22.155169996090148 18-19 20.25561203194573 26.680062624452905 32.35503247529914 20.709292868302228 20-21 21.269794140934284 25.534276854051203 32.68738453417788 20.508544470836632 22-23 20.93886275622291 25.612559953027176 32.539072550584855 20.90950474016506 24-25 20.56853043047996 25.76859640442025 32.7634834240475 20.899389741052286 26-27 20.14615638403167 26.111679313757836 32.67419993401518 21.067964368195316 28-29 20.02257198532821 26.248842938252093 32.85362364514463 20.874961431275068 30-31 20.12126511689297 26.063915790863046 32.70577947897246 21.10903961327152 32-33 19.855698712903795 26.12684851994661 32.66198421694361 21.35546855020599 34-35 20.478940161351524 26.13515252503588 32.38084239354594 21.00506492006665 36-37 20.45069920726819 25.938461842977745 32.21945112476866 21.391387824985404 38-39 20.21360648571971 26.254958721989922 32.346619821692194 21.184814970598183 40-41 20.392713118201534 26.18972612342177 32.364113179227935 21.053447579148767 42-43 20.14602465526997 26.397272182897037 32.02034315464064 21.436360007192356 44-45 20.25673932479735 26.62029171024285 31.652474852280864 21.470494112678935 46-47 20.564214818639346 26.843587260793473 30.901994810490528 21.690203110076656 48-49 20.618606024710584 27.024418572038993 30.722262458817067 21.634712944433353 50-51 20.642010345424996 27.460915908359663 30.359944890399543 21.537128855815794 52-53 20.809842883548985 27.622788486928968 29.99240823871138 21.57496039081067 54-55 20.574295287287867 27.49992163901486 30.156771461424125 21.769011612273147 56-57 20.661557718090922 27.680672573785387 29.87943343112567 21.778336276998022 58-59 20.76843797666653 27.12586057632848 30.13480644762337 21.97089499938162 60-61 20.621902649394343 27.254376489408195 30.503904414008066 21.6198164471894 62-63 20.627639975391844 27.263101122705972 30.54413387365723 21.565125028244953 64-65 20.49742908123048 27.166316511597994 30.43611621410361 21.90013819306792 66-67 20.48055863415664 27.34045149883893 30.29293988811484 21.886049978889595 68-69 20.509441524234262 27.14580471078374 30.354554016008283 21.990199748973712 70-71 20.427319982057572 27.331690809783897 30.236945565846064 22.004043642312464 72-73 20.570966784312432 27.199755912524324 29.840518520961833 22.388758782201403 74-75 20.336324608003377 27.532041714015232 30.029077102977148 22.102556575004247 76-77 20.53359771819587 27.326056150989714 29.9193153267886 22.221030804025823 78-79 20.52646687145511 27.214866189120258 29.784510206977107 22.474156732447533 80-81 20.32462391132225 27.373152546846136 29.817234098706784 22.484989443124835 82-83 20.46653404985996 27.258197005235395 29.75746299430276 22.51780595060189 84-85 20.400677526664833 27.025859154279154 29.694073950683052 22.879389368372962 86-87 20.378104135328424 27.12044440925765 29.800853719684373 22.700597735729556 88-89 20.46045382265418 26.928152444294884 29.57814551620842 23.03324821684251 90-91 20.17689422306091 26.940574493845652 30.00339800540382 22.87913327768962 92-93 20.48576117704304 26.89417993781597 29.76800574006416 22.852053145076823 94-95 20.142014762277842 26.8470578532844 29.6447981526535 23.366129231784257 96-97 20.169347842579736 26.901864673459492 29.498982395608962 23.42980508835181 98-99 20.081876630852513 27.02638063726541 29.507420688199144 23.384322043682932 100-101 20.37201953935412 26.671350306580514 29.73006402073995 23.22656613332542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 353.5 2 167.0 3 184.5 4 151.5 5 164.0 6 287.0 7 595.0 8 1034.0 9 1228.5 10 1021.0 11 804.0 12 800.0 13 832.0 14 847.0 15 872.5 16 905.5 17 930.5 18 949.0 19 972.5 20 1063.0 21 1172.5 22 1290.0 23 1492.5 24 1705.5 25 2007.5 26 2434.5 27 2824.0 28 3091.0 29 3468.0 30 3970.5 31 4474.5 32 5178.0 33 5875.0 34 6541.5 35 7190.5 36 7967.0 37 8728.0 38 8891.0 39 8817.5 40 8968.0 41 9402.0 42 9710.0 43 10187.0 44 10797.5 45 10967.5 46 10711.5 47 10783.5 48 11602.0 49 11453.0 50 10561.5 51 9898.0 52 9227.0 53 8771.0 54 8427.0 55 7777.5 56 6938.0 57 6405.0 58 5867.0 59 5043.5 60 4225.5 61 3509.0 62 2822.0 63 2138.0 64 1552.0 65 1181.0 66 888.5 67 668.5 68 473.0 69 310.5 70 231.5 71 175.0 72 129.5 73 102.5 74 75.0 75 63.5 76 49.5 77 30.0 78 22.0 79 13.5 80 9.0 81 8.0 82 5.5 83 2.5 84 3.5 85 4.0 86 2.5 87 2.5 88 1.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34763143167528066 2 0.3249168779010435 3 0.3051650920104026 4 0.2926556276129967 5 0.2873884847088258 6 0.2287915198999243 7 0.21957401981762517 8 0.2380090199822234 9 0.2357046449616486 10-11 0.23389406458833986 12-13 0.229614510978701 14-15 0.22928531454719034 16-17 0.22698093952661552 18-19 0.2282977252526583 20-21 0.21397768048194357 22-23 0.20278500181058037 24-25 0.20410178753662309 26-27 0.22056160911215722 28-29 0.24344076110214963 30-31 0.23537544853013795 32-33 0.23817361819797872 34-35 0.2320834842150311 36-37 0.21101491259834745 38-39 0.21150870724561346 40-41 0.2049247786153998 42-43 0.22072620732791257 44-45 0.21759884122856107 46-47 0.21677585014978437 48-49 0.23093129670474374 50-51 0.24278236823912827 52-53 0.2666491095236527 54-55 0.22566415380057278 56-57 0.18978174276590842 58-59 0.18352701056720547 60-61 0.19340290351252593 62-63 0.20360799288935708 64-65 0.18797116239259967 66-67 0.19817625176943082 68-69 0.20229120716331433 70-71 0.19027553741317443 72-73 0.19751785890640947 74-75 0.20097442143727162 76-77 0.22270138591697664 78-79 0.22813312703690294 80-81 0.21397768048194357 82-83 0.21101491259834745 84-85 0.20031602857425024 86-87 0.20607696612568718 88-89 0.21595285907100767 90-91 0.21430687691345426 92-93 0.20986272508806006 94-95 0.20821674293050665 96-97 0.19982223392698423 98-99 0.2077229482832406 100-101 0.1932383052967706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 303770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.1870429427066 #Duplication Level Percentage of deduplicated Percentage of total 1 84.62529077778956 56.857230455263796 2 9.141716749148781 12.284098315902384 3 2.511801403306557 5.062815262425251 4 1.1257071740376443 3.025317449719204 5 0.659125346832004 2.2142341491114115 6 0.39662889428516857 1.5988993531593512 7 0.29730948086821113 1.3982741400856413 8 0.2034298163891237 1.0934278247650375 9 0.1394659637516598 0.8433275126065854 >10 0.8431641918675052 9.757638697640532 >50 0.04410798395780837 2.0164869128067493 >100 0.010291862923488474 1.2476271901738551 >500 4.900887106423082E-4 0.1972363913734862 >1k 0.0014702661319269247 2.403386344966738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3149 1.036639562827139 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2454 0.8078480429272147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.5583171478421174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 599 0.19718866247489877 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 446 0.14682160845376435 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.1402376798235507 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.291964315106824E-4 6 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 7 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 8 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 9 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 10-11 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 12-13 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 14-15 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 16-17 3.291964315106824E-4 0.0 0.0 0.0 3.291964315106824E-4 18-19 3.291964315106824E-4 0.0 0.0 0.0 4.937946472660236E-4 20-21 3.291964315106824E-4 0.0 0.0 0.0 6.583928630213648E-4 22-23 3.291964315106824E-4 0.0 0.0 6.583928630213648E-4 6.583928630213648E-4 24-25 3.291964315106824E-4 0.0 0.0 6.583928630213648E-4 6.583928630213648E-4 26-27 3.291964315106824E-4 0.0 0.0 0.001481383941798071 6.583928630213648E-4 28-29 4.937946472660236E-4 0.0 0.0 0.003950357178128189 6.583928630213648E-4 30-31 6.583928630213648E-4 0.0 0.0 0.009052901866543766 6.583928630213648E-4 32-33 6.583928630213648E-4 0.0 0.0 0.012015669750139908 6.583928630213648E-4 34-35 6.583928630213648E-4 0.0 0.0 0.014649241202225369 6.583928630213648E-4 36-37 6.583928630213648E-4 0.0 0.0 0.018105803733087532 6.583928630213648E-4 38-39 6.583928630213648E-4 0.0 0.0 0.026500312736609934 8.22991078776706E-4 40-41 6.583928630213648E-4 0.0 0.0 0.039668169997037234 9.875892945320473E-4 42-43 6.583928630213648E-4 0.0 0.0 0.06715607202817922 9.875892945320473E-4 44-45 6.583928630213648E-4 0.0 0.0 0.09201040260723574 9.875892945320473E-4 46-47 6.583928630213648E-4 0.0 0.0 0.14468183164894494 9.875892945320473E-4 48-49 6.583928630213648E-4 0.0 0.0 0.20508937683115513 9.875892945320473E-4 50-51 6.583928630213648E-4 0.0 0.0 0.2496954933008526 9.875892945320473E-4 52-53 6.583928630213648E-4 0.0 0.0 0.3031899134213385 9.875892945320473E-4 54-55 6.583928630213648E-4 0.0 0.0 0.36771241399743226 9.875892945320473E-4 56-57 6.583928630213648E-4 0.0 0.0 0.4928070579714916 9.875892945320473E-4 58-59 6.583928630213648E-4 0.0 0.0 0.6340323270895744 9.875892945320473E-4 60-61 6.583928630213648E-4 0.0 0.0 0.7782203640912533 9.875892945320473E-4 62-63 6.583928630213648E-4 0.0 0.0 0.9347532672745827 9.875892945320473E-4 64-65 6.583928630213648E-4 0.0 0.0 1.1039602330710734 9.875892945320473E-4 66-67 6.583928630213648E-4 0.0 0.0 1.3515159495671067 9.875892945320473E-4 68-69 6.583928630213648E-4 0.0 0.0 1.6440069789643479 9.875892945320473E-4 70-71 6.583928630213648E-4 0.0 0.0 1.885637159693189 9.875892945320473E-4 72-73 6.583928630213648E-4 0.0 0.0 2.140928992329723 9.875892945320473E-4 74-75 6.583928630213648E-4 0.0 0.0 2.422227343055601 9.875892945320473E-4 76-77 6.583928630213648E-4 0.0 0.0 2.732165783322909 9.875892945320473E-4 78-79 8.22991078776706E-4 0.0 0.0 3.073707081015242 9.875892945320473E-4 80-81 9.875892945320473E-4 0.0 0.0 3.513842709945024 9.875892945320473E-4 82-83 9.875892945320473E-4 0.0 0.0 3.9398228923198473 9.875892945320473E-4 84-85 9.875892945320473E-4 0.0 0.0 4.379629324818119 9.875892945320473E-4 86-87 9.875892945320473E-4 0.0 0.0 4.828159462751424 9.875892945320473E-4 88-89 9.875892945320473E-4 0.0 0.0 5.530500049379464 9.875892945320473E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1135 0.0 55.311768 1 GTATCAA 2075 0.0 41.944256 1 TATCAAC 2680 0.0 32.10996 2 GTAAGTA 60 0.003928538 31.696016 2 ATCAACG 2735 0.0 31.638075 3 TGGTATC 500 0.0 31.37906 2 TCAACGC 2815 0.0 30.744026 4 GTACATG 2090 0.0 30.720407 1 GTGGTAT 520 0.0 30.182142 1 CAACGCA 2895 0.0 29.889507 5 CATATAC 160 1.875378E-9 29.715017 3 AACGCAG 2960 0.0 29.384066 6 TACATGG 2140 0.0 29.326223 2 CATGGGA 1280 0.0 28.605429 4 ACATGGG 2105 0.0 28.458658 3 GTATATG 105 6.830611E-5 27.176996 1 AGAGCGA 70 0.008344185 27.168016 5 CGGTGTA 35 0.008411414 27.127682 80-81 ACGCAGA 3275 0.0 26.412685 7 ATGGGAT 455 0.0 26.12309 5 >>END_MODULE