FastQCFastQC Report
Thu 26 May 2016
SRR937741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303770
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30661.0093162590117524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.8898179543733746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.6264608091648287No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.20278500181058037No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.16328143002929846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4320.1422128584126148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.040.4957351
TATAGGG550.002575358834.548992
GTATCAA26550.031.8593221
GTATAGA753.0653842E-431.6803381
ACTGTCG600.003944440731.6699078
GGCCCGC1252.1431697E-730.4031099
TAAGGTG1751.5097612E-1029.8601975
GTAAGGT1601.891749E-929.6905354
CGTGCAA650.00583447629.2337599
TGACCGT650.00583447629.2337595
AGGTGTG1802.0918378E-1029.0307457
AAGGTGT1503.3216565E-828.4982246
GACGAAT753.3331162E-828.49822470-71
GTATAGG856.38012E-427.9532381
AGGTAAG1554.5596607E-827.5834642
AGGCCCG1405.7773104E-727.1456348
AGCGATC350.00839106327.14116572-73
GTGTAGG1806.6265784E-926.400281
ATCCTAG1309.956029E-625.5879631
GGTGTGC2059.495125E-1025.490418
Produced by FastQC (version 0.11.5)