##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937741_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303770 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26377193271225 33.0 31.0 34.0 31.0 34.0 2 32.45723079961813 34.0 31.0 34.0 31.0 34.0 3 32.501007341080424 34.0 31.0 34.0 31.0 34.0 4 36.12118708233203 37.0 35.0 37.0 35.0 37.0 5 36.048046219178985 37.0 35.0 37.0 35.0 37.0 6 36.10534285808342 37.0 35.0 37.0 35.0 37.0 7 36.05384995226652 37.0 35.0 37.0 35.0 37.0 8 36.02543700826283 37.0 35.0 37.0 35.0 37.0 9 37.711245350100405 39.0 37.0 39.0 35.0 39.0 10-11 37.63801395792869 39.0 37.0 39.0 35.0 39.0 12-13 37.52783191230207 39.0 37.0 39.0 35.0 39.0 14-15 38.69432629950291 40.0 38.0 41.0 34.5 41.0 16-17 38.65517496790335 40.0 38.0 41.0 34.5 41.0 18-19 38.69333377226191 40.0 38.0 41.0 34.5 41.0 20-21 38.616900944793755 40.0 38.0 41.0 34.0 41.0 22-23 38.554735490667284 40.0 38.0 41.0 34.0 41.0 24-25 38.54129769233302 40.0 38.0 41.0 34.0 41.0 26-27 38.323927642624355 40.0 38.0 41.0 34.0 41.0 28-29 38.276357112288906 40.0 38.0 41.0 34.0 41.0 30-31 38.23811930078678 40.0 37.5 41.0 34.0 41.0 32-33 38.031145274385224 40.0 37.0 41.0 34.0 41.0 34-35 37.845415939691215 40.0 37.0 41.0 33.0 41.0 36-37 37.63586265924877 40.0 36.5 41.0 33.0 41.0 38-39 37.44736478256576 40.0 36.0 41.0 33.0 41.0 40-41 37.22061921848767 40.0 36.0 41.0 32.5 41.0 42-43 36.940255785627286 40.0 36.0 41.0 31.5 41.0 44-45 36.4965138097903 39.5 35.0 41.0 30.5 41.0 46-47 35.97342891003062 39.0 35.0 41.0 29.5 41.0 48-49 35.47652664845113 39.0 35.0 41.0 27.0 41.0 50-51 35.14655002139777 39.0 35.0 40.5 26.0 41.0 52-53 34.852248411627215 39.0 35.0 40.0 24.0 41.0 54-55 34.58114198242091 38.0 34.5 40.0 23.5 41.0 56-57 34.304584060308784 38.0 34.0 40.0 23.0 41.0 58-59 34.01772393587254 38.0 34.0 40.0 22.0 41.0 60-61 33.65560786121078 37.5 33.5 40.0 20.5 41.0 62-63 33.23359943378214 37.0 33.0 40.0 18.5 41.0 64-65 32.82931329624387 36.0 33.0 39.0 16.5 41.0 66-67 32.386840372650354 36.0 32.0 39.0 11.5 40.0 68-69 31.89963459196102 35.0 31.5 39.0 8.0 40.0 70-71 31.46367152780064 35.0 31.0 38.0 7.0 40.0 72-73 30.998248674984364 35.0 31.0 37.0 2.0 39.5 74-75 30.501581788853407 35.0 30.0 37.0 2.0 39.0 76-77 29.275385982815948 33.5 28.5 35.5 2.0 38.0 78-79 29.85332982190473 34.0 30.0 36.0 2.0 38.0 80-81 29.67310135958126 34.0 30.0 35.5 2.0 37.0 82-83 29.28952496954933 34.0 29.5 35.0 2.0 37.0 84-85 28.889246798564706 34.0 29.0 35.0 2.0 36.5 86-87 28.544895809329425 34.0 29.0 35.0 2.0 36.0 88-89 28.16131612733318 34.0 29.0 35.0 2.0 36.0 90-91 27.690864799025576 33.5 27.0 35.0 2.0 35.0 92-93 27.240739704381603 33.0 26.5 35.0 2.0 35.0 94-95 26.762302070645553 33.0 25.5 35.0 2.0 35.0 96-97 26.282888369490074 33.0 25.0 35.0 2.0 35.0 98-99 25.508216742930507 32.5 22.0 34.0 2.0 35.0 100-101 23.156063798268427 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22146095046692693 1101 2 0.10053811218235609 1101 3 0.18776566649744098 1101 4 0.13508655099983002 1101 5 0.004383963289001258 1101 6 0.0715826361833436 1101 7 0.14470058315320244 1101 8 0.19216344185566925 1101 9 0.23317191646481206 1101 10-11 0.28709392131777633 1101 12-13 0.14461658116142928 1101 14-15 0.20854240853783068 1101 16-17 0.19838499938889953 1101 18-19 0.27373867821155784 1101 20-21 0.4141716514552982 1101 22-23 0.3626968304491882 1101 24-25 0.2417074095356213 1101 26-27 0.19210350268910048 1101 28-29 0.2123257999966981 1101 30-31 0.42053637460048066 1101 32-33 0.4231941813072453 1101 34-35 0.2819457611000473 1101 36-37 0.052553539776262426 1101 38-39 0.29255552892097825 1101 40-41 0.1838981731469147 1101 42-43 0.25009314533089366 1101 44-45 0.29026349760102477 1101 46-47 0.6569937729679154 1101 48-49 0.6511928156298907 1101 50-51 0.6607350055439625 1101 52-53 0.6789819691114545 1101 54-55 0.7117906934311691 1101 56-57 0.7517924017015787 1101 58-59 0.8328800341680989 1101 60-61 0.7676766026021085 1101 62-63 0.8754566863096471 1101 64-65 0.9528340738491394 1101 66-67 1.1329175837967504 1101 68-69 1.28394192069727 1101 70-71 1.1464133715688867 1101 72-73 0.9351569779762592 1101 74-75 1.2012898008866557 1101 76-77 1.1249853455655625 1101 78-79 1.2393834237640924 1101 80-81 1.4163126540625122 1101 82-83 1.269751808718084 1101 84-85 1.2716899682237468 1101 86-87 1.427091412220669 1101 88-89 1.7581303379196669 1101 90-91 1.5397285788222597 1101 92-93 1.5134255093151197 1101 94-95 1.9227425515050989 1101 96-97 1.9573435086812232 1101 98-99 1.779141070151347 1101 100-101 1.915133135915685 1102 1 0.06634731410328953 1102 2 -0.07005847872673598 1102 3 0.03263173792601748 1102 4 0.05931544710372094 1102 5 0.05332876848380863 1102 6 0.11360779852100933 1102 7 -0.1423935726909562 1102 8 -0.04277973996251205 1102 9 -0.022956330288430138 1102 10-11 0.03735772001907378 1102 12-13 0.11820627271987405 1102 14-15 0.03750953516121314 1102 16-17 0.11519709354474372 1102 18-19 -0.025195980879345825 1102 20-21 -0.15336081607716778 1102 22-23 -0.015113640330028488 1102 24-25 -0.2092970547500954 1102 26-27 -0.047872146661539716 1102 28-29 0.012325799996695253 1102 30-31 0.09525228369138716 1102 32-33 0.11037965208646483 1102 34-35 0.0424571247364085 1102 36-37 0.2544305689970443 1102 38-39 0.06426819125864114 1102 40-41 0.22809460171833962 1102 42-43 0.2827737459802435 1102 44-45 0.46157031578284347 1102 46-47 0.1749016463445372 1102 48-49 0.29526749095456495 1102 50-51 0.40729547632318486 1102 52-53 0.7699214983322378 1102 54-55 0.7995950765480515 1102 56-57 0.7162911841691084 1102 58-59 0.6122022744278368 1102 60-61 0.3265240051995093 1102 62-63 0.5132509233226372 1102 64-65 0.6142890251478406 1102 66-67 0.7245064636668808 1102 68-69 0.40999711550246687 1102 70-71 0.41060777091953327 1102 72-73 0.5837771078463909 1102 74-75 0.6227082262113335 1102 76-77 0.6347560436175144 1102 78-79 0.5828087484394189 1102 80-81 0.6763370047118649 1102 82-83 0.8928950717050945 1102 84-85 0.7087029552367312 1102 86-87 0.8356851622206669 1102 88-89 0.5036559061014891 1102 90-91 0.6913925398612193 1102 92-93 1.0886182852891437 1102 94-95 1.24082290864796 1102 96-97 1.306846349590316 1102 98-99 1.1198168006708258 1102 100-101 1.3169188502014002 1103 1 0.2010455899653607 1103 2 0.2691117798939544 1103 3 0.15402182413291143 1103 4 0.2027421712416526 1103 5 0.20785463055277376 1103 6 0.2615065054175645 1103 7 0.13540815144697405 1103 8 0.189224570382315 1103 9 0.16228073867708304 1103 10-11 0.08989004760528019 1103 12-13 0.2646232985819452 1103 14-15 0.1157692334370779 1103 16-17 0.25784256768267966 1103 18-19 0.3754236311896193 1103 20-21 0.299009873578008 1103 22-23 0.3738141613941153 1103 24-25 0.08345617800852523 1103 26-27 0.07637354299362897 1103 28-29 0.11685166206566322 1103 30-31 0.18523073196724482 1103 32-33 0.22824064346577444 1103 34-35 0.48157350404675014 1103 36-37 0.4699208707211824 1103 38-39 0.48614319125864114 1103 40-41 0.4016937396493745 1103 42-43 0.33975111666989477 1103 44-45 0.3679280744035367 1103 46-47 0.8040772928962596 1103 48-49 0.4306123185407742 1103 50-51 0.41060905390938984 1103 52-53 0.5892372310908556 1103 54-55 0.8188299903411576 1103 56-57 0.7881392445139355 1103 58-59 0.6632259813243948 1103 60-61 0.6422567638201926 1103 62-63 0.5204438112536707 1103 64-65 0.6985293268719843 1103 66-67 0.7261497826323939 1103 68-69 1.0039626327438462 1103 70-71 0.9440398829884984 1103 72-73 0.8324840043981148 1103 74-75 0.9675627520733983 1103 76-77 0.84760625913475 1103 78-79 0.9541449553359698 1103 80-81 1.0111430391946215 1103 82-83 1.108600890670612 1103 84-85 1.2054701966160408 1103 86-87 1.3033845156689452 1103 88-89 1.0179069836876948 1103 90-91 1.2391296088267367 1103 92-93 1.3825029835650078 1103 94-95 1.1423854086479572 1103 96-97 0.8488991513144519 1103 98-99 1.1781950334294464 1103 100-101 1.6628240226151938 1104 1 -0.11233858333260827 1104 2 0.023090559734804117 1104 3 0.06239936613114594 1104 4 0.1619596778729573 1104 5 -0.07695969305465411 1104 6 -0.06928482968412197 1104 7 0.059369247821862814 1104 8 0.01355038824262067 1104 9 0.08834174663464722 1104 10-11 0.05117983540368698 1104 12-13 0.24931604836089605 1104 14-15 0.12234527234070214 1104 16-17 0.03516904867294812 1104 18-19 0.23644264091552714 1104 20-21 0.21586995315360014 1104 22-23 0.12845607120843994 1104 24-25 0.02101143883965051 1104 26-27 0.135060545646148 1104 28-29 -0.10145625128535585 1104 30-31 -0.035757331693226035 1104 32-33 0.28596058157364723 1104 34-35 0.1061590478133283 1104 36-37 0.07632560104833175 1104 38-39 0.30104703741248784 1104 40-41 0.6336234478721892 1104 42-43 0.6638835216212655 1104 44-45 0.3318427516802771 1104 46-47 0.6297293707035152 1104 48-49 0.7380559524930277 1104 50-51 1.0481808929898548 1104 52-53 0.9883309534604408 1104 54-55 0.9689460380865142 1104 56-57 1.299965062374234 1104 58-59 1.1224987487868177 1104 60-61 0.9715560564815604 1104 62-63 0.7740081348610985 1104 64-65 0.9499661084811706 1104 66-67 0.7499672008463669 1104 68-69 0.6585548078101588 1104 70-71 1.1415973542528626 1104 72-73 1.2262450565643412 1104 74-75 1.3288580659549218 1104 76-77 1.0832937038739203 1104 78-79 1.0384177227983926 1104 80-81 1.0182440559939145 1104 82-83 0.7734439499102237 1104 84-85 0.7615875706213444 1104 86-87 0.8030529506822077 1104 88-89 0.9759916432809739 1104 90-91 0.9905111296048119 1104 92-93 1.107027740417351 1104 94-95 1.1792443830069281 1104 96-97 1.4488936252313387 1104 98-99 1.7503656788759585 1104 100-101 1.8169188502014002 1105 1 -9.723376380037507E-4 1105 2 -0.30579386429887734 1105 3 -0.10249730436254367 1105 4 -0.11358567229925853 1105 5 0.03445439037434994 1105 6 -0.13075631839441115 1105 7 -0.12557143338747068 1105 8 -0.1787094663306732 1105 9 0.05806046075634441 1105 10-11 -0.1885828894336612 1105 12-13 -0.1056160220562461 1105 14-15 -0.24418744866962783 1105 16-17 -0.4208919922761467 1105 18-19 -0.23982984281964548 1105 20-21 0.2600720197437312 1105 22-23 -0.22673910674793518 1105 24-25 0.09209831216532649 1105 26-27 -0.2624710272585631 1105 28-29 -0.34215181194360156 1105 30-31 -0.192760776010104 1105 32-33 0.07133658616606908 1105 34-35 0.1908557565772 1105 36-37 -0.23713115363977266 1105 38-39 -0.17321626147767688 1105 40-41 -0.2865353734060392 1105 42-43 -0.4037351159600533 1105 44-45 0.3701368580714046 1105 46-47 -0.29176890713804937 1105 48-49 -0.9598009170056301 1105 50-51 -1.2117693557663713 1105 52-53 -0.5495086944538343 1105 54-55 -0.9593212791733379 1105 56-57 -0.7769340956816322 1105 58-59 -0.669646295223906 1105 60-61 -0.7003727609696497 1105 62-63 -0.5867840580206405 1105 64-65 -0.6423846377874796 1105 66-67 -0.9150729828505355 1105 68-69 -0.7996110934527572 1105 70-71 -1.501748416891413 1105 72-73 -0.8260176682730105 1105 74-75 -1.3121914939379202 1105 76-77 -1.3617885770292553 1105 78-79 -1.0727571719585924 1105 80-81 -0.7233365027508221 1105 82-83 -0.5665149096381867 1105 84-85 -0.6034860994896363 1105 86-87 -0.5186797320579402 1105 88-89 -0.20677242101293913 1105 90-91 0.2378322164781359 1105 92-93 -0.16433180799443647 1105 94-95 0.11222371710566748 1105 96-97 -0.7751304849868035 1105 98-99 -0.3644688025630032 1105 100-101 0.012503427315827764 1106 1 -0.43400380949221784 1106 2 -0.7218316528840383 1106 3 -0.5812552002762317 1106 4 -0.4115061821097612 1106 5 -0.5353650517409108 1106 6 -0.2627089149621398 1106 7 -0.3987840221291563 1106 8 -0.2649356388389137 1106 9 -0.7933566111873063 1106 10-11 -0.46869916200339645 1106 12-13 -1.1464092469430511 1106 14-15 -0.7833735406814881 1106 16-17 -0.7788074710620023 1106 18-19 -1.1859140286321548 1106 20-21 -0.7415630632681811 1106 22-23 -0.7193183453862062 1106 24-25 -0.6270726867725642 1106 26-27 -1.484213438796381 1106 28-29 -1.4989101550594839 1106 30-31 -0.7967772106906352 1106 32-33 -0.8380490698236471 1106 34-35 -1.524080796611905 1106 36-37 -1.9423829843737437 1106 38-39 -1.9074663593031573 1106 40-41 -1.3010135443490753 1106 42-43 -1.5857743579523387 1106 44-45 -1.9516670437677206 1106 46-47 -1.6100790418577091 1106 48-49 -1.0521341944386862 1106 50-51 -1.1297217287329957 1106 52-53 -1.5299994988587784 1106 54-55 -1.4539688279463263 1106 56-57 -1.4370883804376362 1106 58-59 -2.0470849446732835 1106 60-61 -2.076917006036446 1106 62-63 -2.6706763940930927 1106 64-65 -3.063844752942046 1106 66-67 -3.9057516121758162 1106 68-69 -3.4038371541604526 1106 70-71 -2.9171573273950706 1106 72-73 -3.0995796337266412 1106 74-75 -3.1997900181706918 1106 76-77 -2.807299785034175 1106 78-79 -2.5426828245942907 1106 80-81 -2.1931503548386964 1106 82-83 -3.1616432007668145 1106 84-85 -2.645229629032933 1106 86-87 -3.215043419240004 1106 88-89 -3.225373237156937 1106 90-91 -3.304288639914059 1106 92-93 -3.888128554598495 1106 94-95 -2.976294366632942 1106 96-97 -3.2354552655782243 1106 98-99 -3.6341596740482736 1106 100-101 -3.4267609250794955 1107 1 -0.2283255046886552 1107 2 -0.2494313814112985 1107 3 -0.29315475536257907 1107 4 -0.4245142508828508 1107 5 -0.15820646641552116 1107 6 -0.39493876188167576 1107 7 -0.21919726396612305 1107 8 -0.6406614513719049 1107 9 -0.5397558269327263 1107 10-11 -0.1715873303164983 1107 12-13 -0.3061068564747558 1107 14-15 -0.1853040134965056 1107 16-17 -0.1712175457170062 1107 18-19 0.06934575569783163 1107 20-21 -0.006380950305349131 1107 22-23 -0.5923105782494886 1107 24-25 -0.32157159334069974 1107 26-27 -0.27704580437966086 1107 28-29 -0.606801716781824 1107 30-31 -0.40527204516767057 1107 32-33 -0.7647514301282996 1107 34-35 -0.673880962511916 1107 36-37 -0.3806417883854962 1107 38-39 -0.04678466109034929 1107 40-41 -0.3291411365366912 1107 42-43 0.17054115369835898 1107 44-45 -0.6841721338815816 1107 46-47 -0.9511732278165397 1107 48-49 -1.1060328446159033 1107 50-51 -1.4288884582405714 1107 52-53 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2316 92-93 1.6016444332483317 2316 94-95 1.4247833678316333 2316 96-97 1.9625383138760277 2316 98-99 1.6178238159769514 2316 100-101 2.2865617073442586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 7.0 6 20.0 7 53.0 8 89.0 9 190.0 10 361.0 11 577.0 12 878.0 13 1309.0 14 1725.0 15 2458.0 16 3528.0 17 5007.0 18 4623.0 19 2780.0 20 2104.0 21 2090.0 22 2051.0 23 2021.0 24 2299.0 25 2660.0 26 3163.0 27 3756.0 28 4582.0 29 5466.0 30 6724.0 31 8307.0 32 10603.0 33 14003.0 34 19175.0 35 28279.0 36 43658.0 37 65369.0 38 50031.0 39 3816.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44466399257049 20.066259624440978 15.331923834364112 20.15715254862442 2 16.074332554235113 21.2358034038911 40.06551008987063 22.62435395200316 3 19.5562432103236 26.1602528228594 31.077459920334466 23.206044046482535 4 12.797182078546268 17.695624979425222 37.17681140336438 32.33038153866412 5 12.87457283388094 39.11788138773844 33.50430952177148 14.503236256609139 6 30.671231523850285 37.35786944069526 16.880534615004773 15.090364420449681 7 27.593903282088423 35.43108272706324 19.757053033545116 17.217960957303223 8 27.421075155545317 34.847088257563286 18.869539454192317 18.862297132699084 9 27.75718471211772 16.446653718273694 20.428284557395397 35.36787701221319 10-11 25.09233959903875 27.971985383678437 26.031537018138724 20.90413799914409 12-13 26.584326778406602 25.770146275939037 27.369699842958312 20.27582710269605 14-15 23.389077262402473 26.251934029035123 27.129242519011093 23.229746189551307 16-17 21.023471705566713 30.004279553609635 27.68541989004839 21.286828850775258 18-19 20.934259472627318 27.04595582183889 32.022418276985874 19.997366428547913 20-21 21.97751913868904 25.882782833694627 32.4449953007132 19.69470272690313 22-23 21.704020226228256 26.080608630384116 32.37118289198193 19.844188251405697 24-25 21.458340191592324 26.197122823188597 32.43934555749416 19.905191427724926 26-27 21.08126347670074 26.568729116257632 32.4935394136915 19.856467993350122 28-29 20.73329712112982 26.707323095155793 32.58851414744951 19.970865636264875 30-31 20.786614873094774 26.533890772624027 32.51111037956348 20.168383974717713 32-33 20.535437995852124 26.886460150771967 32.398525199986835 20.179576653389077 34-35 21.208644698291472 26.66244197912895 32.254995555848176 19.87391776673141 36-37 21.26392510073477 26.522378920755312 31.96633615127334 20.247359827236576 38-39 21.105770813444384 26.945386312012374 32.06340323270896 19.88543964183428 40-41 21.203846356429906 26.82145584788391 31.930288904690425 20.04440889099576 42-43 20.956981599294004 27.037809038342488 31.544137985537596 20.461071376825913 44-45 21.173091483688317 27.354248279948646 31.221977153767654 20.250683082595387 46-47 21.422622378773415 27.78648319452217 30.304177502715874 20.486716923988542 48-49 21.73930934588669 27.907627481318105 29.821904730552724 20.531158442242486 50-51 21.60499720183033 28.273858511373735 29.572867630114892 20.54827665668104 52-53 22.019784705533795 28.339368601244363 29.33156664581756 20.309280047404286 54-55 21.631168318135433 28.249333377226193 29.456990486223127 20.66250781841525 56-57 21.64746354149521 28.620502353754485 29.1803008855384 20.551733219211904 58-59 21.778977515883728 28.062020607696613 29.210257760805874 20.94874411561379 60-61 21.857820061230537 28.085558152549627 29.60479968397143 20.451822102248414 62-63 21.511999209928565 28.316324851038615 29.72396879217829 20.447707146854526 64-65 21.53932251374395 28.05362609869309 29.525627942193104 20.88142344536985 66-67 21.39974322678342 28.288013957928698 29.320373967146196 20.99186884814169 68-69 21.50689666524015 28.083582973960564 29.3159298153208 21.093590545478488 70-71 21.50195871876749 28.338874806597097 29.192645751720054 20.966520722915362 72-73 21.490107647233103 28.205385653619512 28.927313427922442 21.37719327122494 74-75 21.586891398097244 28.387760476676434 28.826743918095925 21.198604207130394 76-77 21.57043157652171 28.202422885735917 29.000724232149324 21.226421305593046 78-79 21.581788853408828 28.26892056490108 28.79695164104421 21.352338940645886 80-81 21.40435197682457 28.63202422885736 28.72321164038582 21.24041215393225 82-83 21.57198397466496 28.42801602533504 28.55525086496647 21.44474913503353 84-85 21.493470367285667 28.183093074013478 28.700756166692454 21.6226803920084 86-87 21.37192612832077 28.293445699048625 28.649800836158935 21.684827336471674 88-89 21.316291931395465 28.02959475919281 28.500510254468843 22.153603054942884 90-91 21.196048509630977 28.231492410287306 28.690612000322652 21.881847079759066 92-93 21.292478375019545 28.049842390722862 28.727685736154662 21.929993498102927 94-95 20.945945945945947 28.05132172367252 28.620173157322977 22.382559173058564 96-97 21.152187510287387 28.121440563584294 28.40240971787866 22.32396220824966 98-99 21.03269770465899 28.30338992815229 28.28643617240159 22.377476194787132 100-101 20.866347187491254 28.296460431938918 28.303868485645662 22.533323894924166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 229.5 2 139.0 3 176.0 4 150.5 5 135.5 6 274.5 7 603.5 8 951.5 9 1089.0 10 959.0 11 790.5 12 762.0 13 838.5 14 917.5 15 927.5 16 925.0 17 948.5 18 1012.0 19 1059.0 20 1118.0 21 1174.0 22 1316.5 23 1557.5 24 1743.0 25 1998.0 26 2419.0 27 2903.5 28 3163.0 29 3501.5 30 4108.0 31 4690.5 32 5322.0 33 5944.0 34 6565.5 35 7144.5 36 7938.0 37 8619.0 38 8872.0 39 8871.0 40 8837.5 41 9178.0 42 9612.5 43 10186.5 44 10798.0 45 11136.5 46 11024.5 47 10889.5 48 11597.0 49 11514.0 50 10647.0 51 9894.0 52 9172.0 53 8776.0 54 8345.5 55 7712.5 56 6942.5 57 6258.0 58 5687.5 59 5115.0 60 4268.0 61 3408.5 62 2760.0 63 2133.5 64 1540.5 65 1154.5 66 877.5 67 701.0 68 487.0 69 298.0 70 218.0 71 150.5 72 130.5 73 95.0 74 58.5 75 42.5 76 31.5 77 20.0 78 11.0 79 12.0 80 9.0 81 3.0 82 3.5 83 4.0 84 2.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03818678605523916 2 0.0 3 0.0 4 0.0 5 0.007900714356256378 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00954669651380979 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.645982157553412E-4 22-23 0.0019751785890640946 24-25 0.0 26-27 0.0016459821575534122 28-29 0.0016459821575534122 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.583928630213648E-4 38-39 0.0 40-41 6.583928630213648E-4 42-43 0.028310893109918685 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.291964315106824E-4 84-85 3.291964315106824E-4 86-87 0.0 88-89 0.0 90-91 0.012015669750139908 92-93 0.00411495539388353 94-95 0.0 96-97 0.0 98-99 8.229910787767061E-4 100-101 0.015307634065246732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 303770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.60341227103288 #Duplication Level Percentage of deduplicated Percentage of total 1 83.38864256219769 53.871908541674564 2 9.704939961892158 12.539444748474823 3 2.861155572370535 5.545212390402502 4 1.2569596702646453 3.2481553514467376 5 0.6626750815698192 2.1405535748197684 6 0.4385340992925128 1.6998479526900165 7 0.3021351586811132 1.3663273552494926 8 0.22499558315804724 1.1628385934336631 9 0.175973723028435 1.0231652679907084 >10 0.9150194706696693 10.54482074410608 >50 0.051599117069707004 2.341356461308839 >100 0.015326470416744583 1.7809359268709406 >500 5.108823472248195E-4 0.20330921626250917 >1k 0.0015326470416744584 2.5321238752694324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 1.0093162590117524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.8898179543733746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1903 0.6264608091648287 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.20278500181058037 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 496 0.16328143002929846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 432 0.1422128584126148 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.291964315106824E-4 0.0 0.0 0.0 0.0 14-15 3.291964315106824E-4 0.0 0.0 0.0 0.0 16-17 3.291964315106824E-4 0.0 0.0 0.0 0.0 18-19 3.291964315106824E-4 0.0 0.0 0.0 0.0 20-21 3.291964315106824E-4 0.0 0.0 0.0 0.0 22-23 3.291964315106824E-4 0.0 0.0 6.583928630213648E-4 0.0 24-25 3.291964315106824E-4 0.0 0.0 6.583928630213648E-4 0.0 26-27 3.291964315106824E-4 0.0 0.0 0.001481383941798071 0.0 28-29 9.875892945320473E-4 0.0 0.0 0.00411495539388353 0.0 30-31 9.875892945320473E-4 0.0 0.0 0.00954669651380979 0.0 32-33 9.875892945320473E-4 0.0 0.0 0.012674062613161273 0.0 34-35 9.875892945320473E-4 0.0 0.0 0.015307634065246732 0.0 36-37 9.875892945320473E-4 0.0 0.0 0.01892879481186424 0.0 38-39 0.0013167857260427296 0.0 0.0 0.02732330381538664 0.0 40-41 0.0013167857260427296 0.0 0.0 0.040491161075813936 0.0 42-43 0.0013167857260427296 0.0 0.0 0.06797906310695592 0.0 44-45 0.0013167857260427296 0.0 0.0 0.09233959903874642 0.0 46-47 0.0013167857260427296 0.0 0.0 0.14550482272772164 0.0 48-49 0.0013167857260427296 0.0 0.0 0.2068999572044639 3.291964315106824E-4 50-51 0.0013167857260427296 0.0 0.0 0.2518352701056721 3.291964315106824E-4 52-53 0.0013167857260427296 0.0 0.0 0.3069756723837114 3.291964315106824E-4 54-55 0.0013167857260427296 0.0 0.0 0.3718273693913158 3.291964315106824E-4 56-57 0.0013167857260427296 0.0 0.0 0.49873259373868384 3.291964315106824E-4 58-59 0.0013167857260427296 0.0 0.0 0.6422622378773415 3.291964315106824E-4 60-61 0.0013167857260427296 0.0 0.0 0.7874378641735524 3.291964315106824E-4 62-63 0.0013167857260427296 0.0 0.0 0.9461105441617013 3.291964315106824E-4 64-65 0.0013167857260427296 0.0 0.0 1.1167988938999902 3.291964315106824E-4 66-67 0.0013167857260427296 0.0 0.0 1.3650130032590448 3.291964315106824E-4 68-69 0.0013167857260427296 0.0 0.0 1.661783586265925 3.291964315106824E-4 70-71 0.0013167857260427296 0.0 0.0 1.9062119366626065 3.291964315106824E-4 72-73 0.0013167857260427296 0.0 0.0 2.1646311353984924 3.291964315106824E-4 74-75 0.0013167857260427296 0.0 0.0 2.447246271850413 3.291964315106824E-4 76-77 0.0013167857260427296 0.0 0.0 2.7604766764328277 3.291964315106824E-4 78-79 0.0013167857260427296 0.0 0.0 3.1041577509299803 3.291964315106824E-4 80-81 0.0013167857260427296 0.0 0.0 3.549560522763933 3.291964315106824E-4 82-83 0.0013167857260427296 0.0 0.0 3.9799848569641503 3.291964315106824E-4 84-85 0.0013167857260427296 0.0 0.0 4.425387628798104 3.291964315106824E-4 86-87 0.0013167857260427296 0.0 0.0 4.873917766731409 3.291964315106824E-4 88-89 0.0013167857260427296 0.0 0.0 5.5871218355993015 3.291964315106824E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1725 0.0 40.495735 1 TATAGGG 55 0.0025753588 34.54899 2 GTATCAA 2655 0.0 31.859322 1 GTATAGA 75 3.0653842E-4 31.680338 1 ACTGTCG 60 0.0039444407 31.669907 8 GGCCCGC 125 2.1431697E-7 30.403109 9 TAAGGTG 175 1.5097612E-10 29.860197 5 GTAAGGT 160 1.891749E-9 29.690535 4 CGTGCAA 65 0.005834476 29.233759 9 TGACCGT 65 0.005834476 29.233759 5 AGGTGTG 180 2.0918378E-10 29.030745 7 AAGGTGT 150 3.3216565E-8 28.498224 6 GACGAAT 75 3.3331162E-8 28.498224 70-71 GTATAGG 85 6.38012E-4 27.953238 1 AGGTAAG 155 4.5596607E-8 27.583464 2 AGGCCCG 140 5.7773104E-7 27.145634 8 AGCGATC 35 0.008391063 27.141165 72-73 GTGTAGG 180 6.6265784E-9 26.40028 1 ATCCTAG 130 9.956029E-6 25.587963 1 GGTGTGC 205 9.495125E-10 25.49041 8 >>END_MODULE