FastQCFastQC Report
Thu 26 May 2016
SRR937740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185605
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20031.0791735136445677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.878747878559306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.5403949247056922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.20419708520783386No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3110.16756014115999032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.16163357668166267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.11098838932140838No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC250.004991872257.0655484
GGTATCA6950.050.674811
ACCCTAC459.5118437E-442.3050961
GTATCAA12250.041.959751
TAGACTT702.0361604E-433.9584055
TATAAGG702.0361604E-433.9584052
CTCCGCA600.003935940631.6773859
TATCAAC16200.031.4010452
TCAACGC16350.031.1213744
ATCAACG16350.031.1045513
CAACGCA16500.030.8301165
GTACATG11450.030.7589451
GTCCAGG1401.657645E-830.595651
AACGCAG16800.030.2713956
TAGGACA804.4491817E-429.7216424
TACATGG11650.029.3820382
GTGTAAG650.005775939729.2881451
GTAGGGC650.00581425429.2485643
GACTCCG650.005821940529.2406647
ACATGGG11100.029.1168143