##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937740_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 185605 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.843258532905903 33.0 31.0 34.0 30.0 34.0 2 31.936817434875138 33.0 31.0 34.0 30.0 34.0 3 31.9561811373616 34.0 31.0 34.0 30.0 34.0 4 35.57281323240215 37.0 35.0 37.0 33.0 37.0 5 35.57006007381267 37.0 35.0 37.0 33.0 37.0 6 35.648877993588535 37.0 35.0 37.0 35.0 37.0 7 35.59905713746936 37.0 35.0 37.0 33.0 37.0 8 35.60506451873603 37.0 35.0 37.0 33.0 37.0 9 37.16464534899383 39.0 37.0 39.0 34.0 39.0 10-11 37.118480105600604 39.0 37.0 39.0 34.0 39.0 12-13 37.022881926672234 39.0 37.0 39.0 33.0 39.0 14-15 38.128248161418064 40.0 38.0 41.0 33.0 41.0 16-17 38.059755933299215 40.0 38.0 41.0 33.0 41.0 18-19 38.06146924921204 40.0 38.0 41.0 33.5 41.0 20-21 38.11404326392069 40.0 38.0 41.0 33.5 41.0 22-23 38.085423345276254 40.0 38.0 41.0 34.0 41.0 24-25 37.99620430484093 40.0 38.0 41.0 33.0 41.0 26-27 37.885512243743435 40.0 38.0 41.0 33.0 41.0 28-29 37.71524743406697 40.0 38.0 41.0 33.0 41.0 30-31 37.405059130950136 40.0 37.0 41.0 32.5 41.0 32-33 37.19337841114194 39.5 36.0 41.0 32.5 41.0 34-35 37.13158858866949 40.0 36.0 41.0 32.0 41.0 36-37 36.95036232860106 40.0 36.0 41.0 31.5 41.0 38-39 36.755286764903964 40.0 35.5 41.0 31.0 41.0 40-41 36.476579294738826 39.0 35.0 41.0 30.5 41.0 42-43 36.127186767597856 39.0 35.0 41.0 30.0 41.0 44-45 35.744430376336844 39.0 35.0 41.0 28.5 41.0 46-47 35.18345680342664 39.0 35.0 40.5 25.0 41.0 48-49 34.67102718137981 39.0 35.0 40.0 23.0 41.0 50-51 33.72419654642924 38.0 33.5 39.5 20.5 40.5 52-53 33.7880418092185 38.0 33.5 39.5 19.5 40.5 54-55 34.03703833409661 38.0 34.0 40.0 18.5 41.0 56-57 33.786444330702295 38.0 34.0 40.0 14.0 41.0 58-59 33.50122841518278 38.0 34.0 40.0 10.5 41.0 60-61 33.16839794186579 38.0 33.0 40.0 8.0 41.0 62-63 32.8063225667412 37.0 33.0 40.0 4.5 41.0 64-65 32.35014142937959 36.5 32.0 39.5 2.0 41.0 66-67 31.860332426389377 36.0 31.5 39.0 2.0 41.0 68-69 31.386086042940654 35.5 31.0 39.0 2.0 40.0 70-71 30.942628161956844 35.0 31.0 38.5 2.0 40.0 72-73 30.4848953422591 35.0 30.0 37.5 2.0 40.0 74-75 29.969125292960857 35.0 29.5 37.0 2.0 39.0 76-77 29.4408474987204 34.0 29.0 36.5 2.0 39.0 78-79 28.937450499717144 34.0 29.0 36.0 2.0 38.5 80-81 28.43933083699254 34.0 28.0 35.5 2.0 37.5 82-83 27.941394358988177 34.0 26.5 35.0 2.0 37.0 84-85 27.46442175587942 34.0 26.0 35.0 2.0 36.5 86-87 26.94433609008378 33.0 25.5 35.0 2.0 36.0 88-89 26.43087740093209 33.0 24.5 35.0 2.0 35.5 90-91 25.88255973707605 32.5 23.5 35.0 2.0 35.0 92-93 25.284973465154494 32.0 19.5 34.5 2.0 35.0 94-95 24.563767139893862 31.5 14.0 34.0 2.0 35.0 96-97 23.770501872255597 31.0 2.0 34.0 2.0 35.0 98-99 22.67181649201261 31.0 2.0 34.0 2.0 35.0 100-101 19.69412461948762 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.01849525967065091 1101 2 -0.32909608365029897 1101 3 -0.323608130662155 1101 4 -0.19110731409232073 1101 5 -0.18025075033073534 1101 6 -0.2668548193004554 1101 7 -0.13466565594271884 1101 8 -0.17689149564527185 1101 9 -0.3899566478526779 1101 10-11 -0.019717682075899745 1101 12-13 -0.0013308727750143134 1101 14-15 -0.040330574266477015 1101 16-17 -0.04613473052359751 1101 18-19 -0.18683938535961175 1101 20-21 -0.08436445439055262 1101 22-23 -0.22246021511980274 1101 24-25 -0.26622690978440744 1101 26-27 -0.16757804438532276 1101 28-29 -0.12017994264604681 1101 30-31 -0.15817284836933254 1101 32-33 -0.005880138961181558 1101 34-35 0.15220829927180546 1101 36-37 0.3640868638714849 1101 38-39 0.1604228441383313 1101 40-41 0.20169829567819164 1101 42-43 0.29683855696661254 1101 44-45 0.4355685789286028 1101 46-47 0.48361923974975696 1101 48-49 0.29546522106849693 1101 50-51 0.40269905418983143 1101 52-53 0.5696681872609659 1101 54-55 0.819661519369923 1101 56-57 1.0621192783953717 1101 58-59 0.9248422499320839 1101 60-61 1.0958896971625265 1101 62-63 1.1932202562471588 1101 64-65 1.059341576421776 1101 66-67 0.9817500432061514 1101 68-69 1.3833982628457555 1101 70-71 1.5044295092252398 1101 72-73 1.4972054751856234 1101 74-75 1.3018555553436464 1101 76-77 1.1619531711722715 1101 78-79 1.4840363514141934 1101 80-81 1.4119891295098164 1101 82-83 1.357587817953707 1101 84-85 1.4518128116494715 1101 86-87 1.5362133655210215 1101 88-89 1.660326661334917 1101 90-91 1.724610571574015 1101 92-93 1.3424391949622638 1101 94-95 1.944102439092486 1101 96-97 1.9972912019174949 1101 98-99 2.815885868284983 1101 100-101 3.374455086226593 1102 1 0.14709650441478317 1102 2 0.1697667536530325 1102 3 -0.15008369539802402 1102 4 0.10064908038776821 1102 5 0.0804451712654668 1102 6 -0.04610242411719412 1102 7 0.12396469476485095 1102 8 0.0162338889025051 1102 9 0.17285648390437558 1102 10-11 0.369499598402264 1102 12-13 0.19735946738495613 1102 14-15 0.44738861796345475 1102 16-17 0.5605910100055382 1102 18-19 0.3295725641877212 1102 20-21 0.654599024558209 1102 22-23 0.2930288197751665 1102 24-25 0.10805068975852805 1102 26-27 0.28483966239150504 1102 28-29 0.20483983409650364 1102 30-31 0.31062384671724175 1102 32-33 0.22709538182638056 1102 34-35 0.5824375996146003 1102 36-37 0.8440766458878315 1102 38-39 1.0237161112561992 1102 40-41 0.8352682174501922 1102 42-43 0.8903468412243285 1102 44-45 1.167824446028888 1102 46-47 1.4080116542702825 1102 48-49 1.6709431590134756 1102 50-51 1.7175640229865223 1102 52-53 1.3497068617797296 1102 54-55 1.4883626268675059 1102 56-57 1.942469985963271 1102 58-59 1.8880163072406901 1102 60-61 2.1479876799347792 1102 62-63 2.3493713725344065 1102 64-65 1.9422934749890608 1102 66-67 2.2372051280264067 1102 68-69 2.3182696041483872 1102 70-71 2.227516658737411 1102 72-73 2.6420673676001485 1102 74-75 2.231656359597846 1102 76-77 2.040620108853556 1102 78-79 2.217039339899735 1102 80-81 2.361552942465778 1102 82-83 2.6051624202214434 1102 84-85 2.6571695621109264 1102 86-87 2.2863699313995482 1102 88-89 2.49400425648302 1102 90-91 2.270742570343465 1102 92-93 2.209668583201694 1102 94-95 2.4746135580166317 1102 96-97 2.3775210516142486 1102 98-99 2.646143735033686 1102 100-101 3.0782104698953994 1103 1 -0.5219273990600151 1103 2 -0.18994272298571957 1103 3 0.12314313700425927 1103 4 -0.06773235454976145 1103 5 -0.12720430519102166 1103 6 -0.01790671270859434 1103 7 -0.11662838115579177 1103 8 -0.3213582666022248 1103 9 -0.5382331609608926 1103 10-11 -0.12672760763420854 1103 12-13 -0.22266003449688299 1103 14-15 -0.297865772238211 1103 16-17 -0.1252054231549522 1103 18-19 -0.09507179985956071 1103 20-21 -0.23983564869413954 1103 22-23 -0.4522483529987511 1103 24-25 -0.389456896838027 1103 26-27 -0.517261802072241 1103 28-29 -0.39235061909442237 1103 30-31 -0.12537535133620992 1103 32-33 0.21294740446313654 1103 34-35 0.06342782133459934 1103 36-37 0.26469305116246034 1103 38-39 -0.02854710947047323 1103 40-41 -0.22541088546324062 1103 42-43 0.041440860343463726 1103 44-45 0.4445096728977447 1103 46-47 -0.06427562485268368 1103 48-49 0.3589631712249357 1103 50-51 0.5575802441779629 1103 52-53 0.9807157652089629 1103 54-55 0.8548932056359817 1103 56-57 1.065113128864681 1103 58-59 1.2401574133801319 1103 60-61 1.1913793670298247 1103 62-63 1.4373303274522513 1103 64-65 1.0584481413155054 1103 66-67 0.8535876200752917 1103 68-69 0.7306478312997413 1103 70-71 1.1898661798477441 1103 72-73 1.5022188261404175 1103 74-75 1.2448611384701955 1103 76-77 1.386947102556455 1103 78-79 1.3521855584483937 1103 80-81 1.5235370311173249 1103 82-83 1.9283377640104575 1103 84-85 2.227540371256765 1103 86-87 1.889019088900028 1103 88-89 1.9493276323134445 1103 90-91 1.3255619956758622 1103 92-93 1.4266816968502773 1103 94-95 1.4945528652441773 1103 96-97 1.6760456522357607 1103 98-99 2.448286208127467 1103 100-101 3.1278400252707215 1104 1 -0.36619997431084883 1104 2 -0.8042821878686617 1104 3 -0.5641478328954044 1104 4 -0.7579497458541127 1104 5 -0.6999467801074104 1104 6 -0.42488831805976446 1104 7 -0.2796718594166592 1104 8 -0.7032562599132604 1104 9 -0.7869789803588816 1104 10-11 -0.739708376864975 1104 12-13 -0.26645184051695026 1104 14-15 -0.1812269762515939 1104 16-17 -0.7620156238238422 1104 18-19 -0.7015726359799572 1104 20-21 -0.5189953476907974 1104 22-23 -0.6011822994870428 1104 24-25 -0.7728181008514099 1104 26-27 -0.9149415679585218 1104 28-29 -0.8208623247800446 1104 30-31 -0.8414079600318658 1104 32-33 -0.907244903229163 1104 34-35 -0.7304684997356432 1104 36-37 -0.9147592899746684 1104 38-39 -0.8726106546209778 1104 40-41 -1.035507039309401 1104 42-43 -1.1584964306264354 1104 44-45 -1.2163389893096124 1104 46-47 -1.2230339860566914 1104 48-49 -1.1809615779389446 1104 50-51 -1.0867550401029789 1104 52-53 -1.172189753185684 1104 54-55 -0.9408843863372667 1104 56-57 -0.8671611186269601 1104 58-59 -0.5601143257503054 1104 60-61 -0.9848330075521119 1104 62-63 -0.7024062946213299 1104 64-65 -0.6781739322630891 1104 66-67 -0.8547108749079904 1104 68-69 -0.8351966503056083 1104 70-71 -0.2985326495836951 1104 72-73 -0.07219588957864431 1104 74-75 0.13909190770096913 1104 76-77 0.08991532998120633 1104 78-79 -0.020306080347591404 1104 80-81 -0.549937049150234 1104 82-83 -0.6345802961902116 1104 84-85 -0.23002651837534316 1104 86-87 0.385779122344843 1104 88-89 0.17560271592548915 1104 90-91 0.44429109266582856 1104 92-93 0.42145594434191835 1104 94-95 -0.2213752284013104 1104 96-97 -0.04186822736290097 1104 98-99 -0.03561847414677999 1104 100-101 0.5173676172439663 1105 1 0.31886030123450126 1105 2 0.32078956873294473 1105 3 0.6195490672194062 1105 4 -0.07841472863252363 1105 5 0.06899696271693045 1105 6 -0.06560301381177425 1105 7 0.13335913943523536 1105 8 0.45740677108559424 1105 9 0.4189624662542073 1105 10-11 0.20277870694673794 1105 12-13 0.23867875649797554 1105 14-15 0.3450359399366931 1105 16-17 0.6257929295630618 1105 18-19 0.12158321935873317 1105 20-21 0.5040743997259653 1105 22-23 0.7290774594107674 1105 24-25 0.4681405673460688 1105 26-27 0.17235033789681609 1105 28-29 0.08829381758506116 1105 30-31 0.3634930158579195 1105 32-33 0.8280449934413241 1105 34-35 0.6901686988866373 1105 36-37 0.5324637295431316 1105 38-39 0.3364880824628358 1105 40-41 0.11184514209129048 1105 42-43 0.6904200446892901 1105 44-45 1.0178386800359647 1105 46-47 0.6944100437940506 1105 48-49 1.2816532326236327 1105 50-51 1.7889984042139062 1105 52-53 1.304381716389507 1105 54-55 1.1922241096443642 1105 56-57 1.5234818205234433 1105 58-59 1.9185380852715141 1105 60-61 2.0036141795891 1105 62-63 1.7082208580767144 1105 64-65 1.4234334156129158 1105 66-67 1.6888095039668691 1105 68-69 1.5470507664567315 1105 70-71 1.9223700484645256 1105 72-73 1.9639694376314552 1105 74-75 2.2003720454736424 1105 76-77 2.1054865125299997 1105 78-79 2.506243862247125 1105 80-81 2.6192291391390867 1105 82-83 2.0919552950841833 1105 84-85 2.482731805390447 1105 86-87 2.7153930718281956 1105 88-89 2.993018043135592 1105 90-91 2.8291935036876446 1105 92-93 3.1620558295313543 1105 94-95 3.131451981702021 1105 96-97 2.772748592384513 1105 98-99 2.528316657885366 1105 100-101 3.4747800741900043 1106 1 -0.2502034708143448 1106 2 -0.23199197807845096 1106 3 0.15333468458711152 1106 4 0.08942787652351569 1106 5 0.334668604507975 1106 6 0.24810119242975048 1106 7 0.1773211475763432 1106 8 0.1977052785482769 1106 9 0.07228675390685169 1106 10-11 -0.14735697826358063 1106 12-13 0.22843452311941803 1106 14-15 -0.16846473848935517 1106 16-17 0.1724686419104131 1106 18-19 0.656250790593468 1106 20-21 0.12571619077074558 1106 22-23 0.047462805408059694 1106 24-25 -0.49502089805420724 1106 26-27 0.00450773274077676 1106 28-29 0.7415502626325505 1106 30-31 0.9639679140940061 1106 32-33 0.7891711806869139 1106 34-35 0.6211049268377948 1106 36-37 0.22601868205330078 1106 38-39 0.03560612859580914 1106 40-41 0.6340296739773166 1106 42-43 0.49014867426866715 1106 44-45 0.9698061155854916 1106 46-47 2.024125104852402 1106 48-49 1.2035663940890302 1106 50-51 1.9529739808760453 1106 52-53 1.6648294775835453 1106 54-55 2.2142729563200803 1106 56-57 2.153468252002412 1106 58-59 2.0392300798441028 1106 60-61 2.0650795798604733 1106 62-63 1.806728320763284 1106 64-65 1.7893085852194268 1106 66-67 1.6949831810360685 1106 68-69 1.4888418112328488 1106 70-71 1.6661963713953263 1106 72-73 1.2029789355961782 1106 74-75 1.861294704903763 1106 76-77 2.6018908544567303 1106 78-79 2.73616245112094 1106 80-81 3.0367325312693474 1106 82-83 2.8833392842293684 1106 84-85 2.9945364186875914 1106 86-87 2.1951759754916935 1106 88-89 1.8774820865548598 1106 90-91 2.149885498260236 1106 92-93 1.953711189097163 1106 94-95 2.118222673696593 1106 96-97 1.7884202341755575 1106 98-99 0.9863220852937822 1106 100-101 1.7051735612999117 1107 1 0.0050262457056931 1107 2 0.22265122817765715 1107 3 -0.1683661951782831 1107 4 0.45472601924100786 1107 5 0.44542129268001673 1107 6 -0.003413959085406759 1107 7 -0.44985382798572715 1107 8 -0.15068181822591242 1107 9 0.3802046424698986 1107 10-11 0.38485221039722006 1107 12-13 0.13307772937552187 1107 14-15 0.2946828665937318 1107 16-17 0.28014215119682717 1107 18-19 0.3491149157156599 1107 20-21 -0.009767680197001027 1107 22-23 0.12532204294470972 1107 24-25 -0.2658908882790314 1107 26-27 0.23598378357166183 1107 28-29 -0.15512617920519034 1107 30-31 0.5192465064693792 1107 32-33 0.4060822266302253 1107 34-35 -0.03764385126582681 1107 36-37 -0.0018405554833478277 1107 38-39 -0.5230448977971562 1107 40-41 -0.3776027795906245 1107 42-43 -0.35403509894737084 1107 44-45 -0.9636249694585004 1107 46-47 -0.40280549143303546 1107 48-49 -0.27913155312504756 1107 50-51 -0.8516692253800642 1107 52-53 -0.6615146084379617 1107 54-55 -0.556010523640829 1107 56-57 -0.5158377108519403 1107 58-59 -1.2965959221109316 1107 60-61 -0.7552527759557535 1107 62-63 -0.09811038891413659 1107 64-65 -0.5216396063739275 1107 66-67 -0.07134135464330882 1107 68-69 -0.6337388339284367 1107 70-71 0.05080047696717571 1107 72-73 -0.6456036645993244 1107 74-75 -0.23073853067785777 1107 76-77 0.062154784075499236 1107 78-79 0.4826922653926893 1107 80-81 0.5080912800474486 1107 82-83 0.11598835558811871 1107 84-85 -0.49475976899568863 1107 86-87 -0.33038609684457043 1107 88-89 -0.08429699457905926 1107 90-91 -0.21120932089909772 1107 92-93 0.6122644637794714 1107 94-95 0.12286587995270182 1107 96-97 -0.26749374431906503 1107 98-99 -0.22208456182254466 1107 100-101 1.0102077743986406 1108 1 0.240360395698886 1108 2 0.7752577342345539 1108 3 0.4061540171532805 1108 4 0.3680239639803631 1108 5 0.11821290830544484 1108 6 0.4311851970327396 1108 7 0.545790652754711 1108 8 0.7774521139913162 1108 9 0.36917973434413653 1108 10-11 0.6775567156374578 1108 12-13 0.27429183036912264 1108 14-15 0.15190350086622573 1108 16-17 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-0.25032446054356683 2316 62-63 0.19075609854106546 2316 64-65 -0.22224444508360364 2316 66-67 -0.3360142937114077 2316 68-69 -0.4812970776560377 2316 70-71 -0.31669504274608684 2316 72-73 0.003925905293151288 2316 74-75 0.11905985641891803 2316 76-77 -0.3606722768564019 2316 78-79 -0.3774107812022933 2316 80-81 -0.7630780747912631 2316 82-83 -1.3234157662756836 2316 84-85 -0.7935948944437179 2316 86-87 -1.4384730144072932 2316 88-89 -1.8211921558693867 2316 90-91 -1.43925591588118 2316 92-93 -1.410809012923039 2316 94-95 -1.8322192454953274 2316 96-97 -1.6983853213799982 2316 98-99 -1.845180439958746 2316 100-101 -1.997856741730395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 935.0 3 154.0 4 129.0 5 161.0 6 180.0 7 180.0 8 256.0 9 306.0 10 379.0 11 546.0 12 772.0 13 1147.0 14 1207.0 15 1693.0 16 2327.0 17 3216.0 18 2821.0 19 1593.0 20 1538.0 21 1591.0 22 1705.0 23 1707.0 24 1818.0 25 2017.0 26 2283.0 27 2761.0 28 3107.0 29 3679.0 30 4412.0 31 5500.0 32 6923.0 33 9197.0 34 12483.0 35 18148.0 36 26044.0 37 35347.0 38 25074.0 39 2268.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1164695571257 19.035046905939254 15.3155736374555 21.532909899479545 2 14.986457043688874 19.927879027069693 41.690139322149356 23.395524607092074 3 18.366784177174125 26.004066007742715 31.231481281224994 24.397668533858166 4 11.69861917583071 16.861841891848055 37.981315080935545 33.45822385138569 5 12.281583841529347 37.719497426581896 34.708165736776095 15.290752995112669 6 29.3599044907705 37.05412369741725 18.104617179776678 15.481354632035568 7 26.64376495497977 34.732282231188464 20.881607873003528 17.742344940828243 8 26.10832914688231 34.030174858605996 19.996110651951966 19.86538534255973 9 26.66994397955821 16.127080508451684 21.091129106319993 36.111846405670114 10-11 24.08948379098842 27.216771053619514 27.14465898011381 21.549086175278255 12-13 25.662536057302752 25.102094834757622 28.093635548449132 21.141733559490497 14-15 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26.38164360777198 32.754970892870645 21.014915378716694 40-41 20.06231337037577 26.148396537666112 32.85734342010767 20.931946671850447 42-43 19.737581145159375 26.275909743899938 32.626024778302245 21.360484332638446 44-45 19.814528259595622 26.624160475723674 32.22503976516275 21.336271499517956 46-47 20.275478373597288 26.85373988899842 31.28408032085128 21.58670141655301 48-49 20.349737048951845 27.112994670750712 30.98581657982664 21.5514517004708 50-51 20.31439915728061 27.32868757259001 30.757367042108957 21.59954622802042 52-53 20.539503721414 27.486323296997206 30.394294282125056 21.57987869946374 54-55 20.294838171225457 27.331952236000184 30.63713243138854 21.736077161385815 56-57 20.397816544373715 27.61121099718699 30.198530324872713 21.79244213356658 58-59 20.503172833741353 27.093921816842066 30.385247605192056 22.017657744224525 60-61 20.4505574602489 26.946521610020785 30.80851982830764 21.794401101422668 62-63 20.236359906276657 27.03994039713647 31.048286957554556 21.675412739032318 64-65 20.13147415736404 26.999716534157635 31.029520942726403 21.839288365751926 66-67 20.171476855213818 27.058896576671916 30.685502484956633 22.084124083157636 68-69 20.17174449429588 26.9216648579774 30.7566260116512 22.149964636075524 70-71 20.074886347684302 26.83247486691071 30.73660738389107 22.356031401513924 72-73 20.11323719601591 26.82862951673043 30.52031330577553 22.537819981478133 74-75 20.078897487268936 27.03953514745352 30.55374047532901 22.327826889948536 76-77 20.071997234614845 26.809652922558413 30.411945167597466 22.706404675229276 78-79 20.20743717073197 26.749187884286673 30.274161623853384 22.76921332112797 80-81 19.930933744454855 26.822979245023205 30.390232443860043 22.855854566661897 82-83 20.050859399675513 26.487380983875624 30.597975882278305 22.86378373417055 84-85 19.952056107312256 26.47064381413103 30.392540621380924 23.18475945717579 86-87 20.01436250199104 26.52473347029905 30.3866806692062 23.07422335850371 88-89 20.047685008924063 26.3266612123225 30.112570103173507 23.513083675579928 90-91 19.805554205612523 26.3665473317026 30.410706668178605 23.417191794506273 92-93 19.943468354156995 26.376845242594733 30.218513439089445 23.461172964158827 94-95 19.671387612511676 26.162103205671617 30.199024853809064 23.967484328007647 96-97 19.76098672628498 26.419715084945295 29.97548304874229 23.843815140027434 98-99 19.87379062853563 26.683264162101004 29.694035432592475 23.748909776770887 100-101 20.053769681133915 26.123373345678047 30.030852783903644 23.792004189284395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 361.0 1 220.0 2 102.5 3 131.0 4 109.5 5 108.5 6 178.0 7 373.0 8 649.0 9 761.5 10 621.0 11 483.0 12 492.0 13 523.5 14 535.5 15 550.5 16 567.5 17 574.0 18 583.0 19 616.0 20 666.0 21 728.5 22 895.5 23 1053.5 24 1119.5 25 1284.5 26 1561.5 27 1837.0 28 1984.0 29 2137.0 30 2500.0 31 2888.0 32 3235.5 33 3624.0 34 4087.0 35 4370.5 36 4826.0 37 5384.0 38 5435.5 39 5370.5 40 5500.0 41 5751.0 42 5971.0 43 6307.0 44 6568.5 45 6605.0 46 6559.5 47 6623.0 48 7103.0 49 7040.5 50 6360.5 51 5921.5 52 5550.5 53 5322.0 54 5179.5 55 4750.5 56 4201.0 57 3761.0 58 3336.0 59 2891.0 60 2481.0 61 2055.0 62 1643.5 63 1255.0 64 919.0 65 711.5 66 525.0 67 394.0 68 280.0 69 180.5 70 134.5 71 90.0 72 64.0 73 49.0 74 41.0 75 31.5 76 23.5 77 20.5 78 12.5 79 8.5 80 8.0 81 7.0 82 4.5 83 2.5 84 1.5 85 1.5 86 3.0 87 2.0 88 0.5 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41324317771611757 2 0.34374073974300257 3 0.35397753293284123 4 0.34643463268769703 5 0.3432019611540637 6 0.2650790657579268 7 0.25107082244551604 8 0.26076883704641574 9 0.26615662293580455 10-11 0.2567279976293742 12-13 0.2602300584574769 14-15 0.2545728832736187 16-17 0.2475687616174133 18-19 0.23760135772204413 20-21 0.23948708278333017 22-23 0.24514425796718836 24-25 0.24595242585059668 26-27 0.2559198297459659 28-29 0.26750356940815173 30-31 0.27127501953072386 32-33 0.2688505158804989 34-35 0.26831173729156005 36-37 0.24649120443953557 38-39 0.23005845747689985 40-41 0.22197677864281676 42-43 0.23867891489992185 44-45 0.24541364726165782 46-47 0.2545728832736187 48-49 0.266426012230274 50-51 0.264001508580049 52-53 0.2842057056652569 54-55 0.26211578351876297 56-57 0.21227876404191695 58-59 0.19422968131246465 60-61 0.21012364968616146 62-63 0.20419708520783386 64-65 0.21416448910320304 66-67 0.21039303898063091 68-69 0.20904609250828374 70-71 0.21066242827510037 72-73 0.22547883947091943 74-75 0.23059723606583876 76-77 0.24729937232294388 78-79 0.23733196842757467 80-81 0.22655639664879718 82-83 0.20931548180275314 84-85 0.20931548180275314 86-87 0.21578082487001965 88-89 0.2324829611271248 90-91 0.22197677864281676 92-93 0.21497265698661133 94-95 0.21658899275342797 96-97 0.23005845747689985 98-99 0.23517685407181918 100-101 0.1996174672018534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 185605.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.89742549952501 #Duplication Level Percentage of deduplicated Percentage of total 1 82.95624328314342 54.66602861471416 2 10.22579882452094 13.477076324239977 3 2.7105679259307696 5.3585834388127465 4 1.285701120690263 3.3889757526136965 5 0.7596266970500704 2.502872183815363 6 0.44826189112248593 1.7723582744712123 7 0.3172417763696511 1.463379142655924 8 0.2170908756576659 1.144458384422215 9 0.18932970989549086 1.1228706407416313 >10 0.850488124068317 9.589698339991642 >50 0.0267635957968774 1.228024397419764 >100 0.009912442887732369 1.25676539395512 >500 0.0 0.0 >1k 0.0029737328663197106 3.0289091121465135 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 1.0791735136445677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.878747878559306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.5403949247056922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.20419708520783386 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 311 0.16756014115999032 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.16163357668166267 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 206 0.11098838932140838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.387785889388756E-4 0.0 24-25 0.0 0.0 0.0 0.0016163357668166267 0.0 26-27 0.0 0.0 0.0 0.0029632822391638157 0.0 28-29 0.0 0.0 0.0 0.00484900730044988 0.0 30-31 0.0 0.0 0.0 0.00862045742302201 0.0 32-33 0.0 0.0 0.0 0.012661296840063577 0.0 34-35 0.0 0.0 0.0 0.017779693434982896 0.0 36-37 0.0 0.0 0.0 0.021281754263085588 0.0 38-39 0.0 0.0 0.0 0.03017160098057703 0.0 40-41 0.0 0.0 0.0 0.04902885159343767 0.0 42-43 0.0 0.0 0.0 0.08243312410764796 0.0 44-45 0.0 0.0 0.0 0.11233533579375556 0.0 46-47 0.0 0.0 0.0 0.18911128471754532 0.0 48-49 0.0 0.0 0.0 0.26642601223027396 0.0 50-51 0.0 0.0 0.0 0.32703860348589747 0.0 52-53 0.0 0.0 0.0 0.41512890277740366 0.0 54-55 0.0 0.0 0.0 0.5037579806578487 0.0 56-57 0.0 0.0 0.0 0.6958325476145578 0.0 58-59 0.0 0.0 0.0 0.8973357398776973 0.0 60-61 0.0 0.0 0.0 1.1026103822634088 0.0 62-63 0.0 0.0 0.0 1.3105789175938147 0.0 64-65 0.0 0.0 0.0 1.5425231001320008 0.0 66-67 0.0 0.0 0.0 1.9005414724818837 0.0 68-69 0.0 0.0 0.0 2.3321031222219233 0.0 70-71 0.0 0.0 0.0 2.7052072950620945 0.0 72-73 0.0 0.0 0.0 3.0656501710622024 0.0 74-75 0.0 0.0 0.0 3.462999380404623 0.0 76-77 0.0 0.0 0.0 3.911532555696237 0.0 78-79 0.0 0.0 0.0 4.40855580399235 0.0 80-81 0.0 0.0 0.0 5.068559575442472 0.0 82-83 0.0 0.0 0.0 5.654481290913499 0.0 84-85 0.0 0.0 0.0 6.280003232671533 0.0 86-87 0.0 0.0 0.0 6.856226933541661 0.0 88-89 0.0 0.0 0.0 7.785350610166752 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGATC 25 0.0049918722 57.065548 4 GGTATCA 695 0.0 50.67481 1 ACCCTAC 45 9.5118437E-4 42.305096 1 GTATCAA 1225 0.0 41.95975 1 TAGACTT 70 2.0361604E-4 33.958405 5 TATAAGG 70 2.0361604E-4 33.958405 2 CTCCGCA 60 0.0039359406 31.677385 9 TATCAAC 1620 0.0 31.401045 2 TCAACGC 1635 0.0 31.121374 4 ATCAACG 1635 0.0 31.104551 3 CAACGCA 1650 0.0 30.830116 5 GTACATG 1145 0.0 30.758945 1 GTCCAGG 140 1.657645E-8 30.59565 1 AACGCAG 1680 0.0 30.271395 6 TAGGACA 80 4.4491817E-4 29.721642 4 TACATGG 1165 0.0 29.382038 2 GTGTAAG 65 0.0057759397 29.288145 1 GTAGGGC 65 0.005814254 29.248564 3 GACTCCG 65 0.0058219405 29.240664 7 ACATGGG 1110 0.0 29.116814 3 >>END_MODULE