FastQCFastQC Report
Thu 26 May 2016
SRR937740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185605
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.9988955038926753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.8582742921796288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.6486894210824062No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.2160502141644891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.19719296355162844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.15732334797015166No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC200.002062927271.323021
GGTATCA12100.042.4401441
TTTAGAC704.2763604E-640.7340353
GGTAAGG756.8680656E-638.0184363
CCTATGC500.001612753337.997958
CATATAG651.3201222E-436.556193
CCATATA550.002567960234.5622142
TAAGGTG1255.5715645E-934.216595
CATATAC702.0409009E-433.945033
AGGTAAG753.0598594E-431.682032
GTAAGGT1201.4879333E-731.6820284
CTATGCT600.00394353631.664969
CGAGTCA300.003948578631.6649682-83
TCGAGTC300.003948578631.6649680-81
ATACAGC600.00394353631.664966
GTATCAA18300.029.620491
TTAGACT856.3686963E-427.9547334
GATTGTG700.008375972527.1413927
TAGAGCG458.9389033E-426.38746618-19
GTACATG13300.024.6681121