FastQCFastQC Report
Thu 26 May 2016
SRR937739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696987
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98081.4071998473429204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76651.099733567484042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59470.8532440346807042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24140.34634792327547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.24261571593157402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.2110512821616472No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.19942983154635596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.16011776403290162No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10240.14691809172911402No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.11822315193827146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.11635798085186669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT459.618825E-442.2254526
GGTATCA39500.038.180831
CGCAGTA500.001614770238.0029078
GTATCAA64050.037.2136081
ATCAACG79750.029.631853
TATCAAC80100.029.5617312
TCAACGC79950.029.5555954
CAACGCA81050.029.1565675
AACGCAG82100.028.8146296
ACGCAGA89750.025.88227
CGCAGAG89450.025.756588
GAGTACT54600.025.14205712-13
GTACATG41450.025.1363811
GCAGAGT92050.024.5628479
GTATAGG3100.024.5550881
TACATGG42450.023.8580472
GTACTTT58100.023.62577414-15
GTGTAGC4300.023.2345371
AGTACTT58100.023.0960612-13
ACATGGG41300.022.5651933
Produced by FastQC (version 0.11.5)