FastQCFastQC Report
Thu 26 May 2016
SRR937739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696987
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97501.398878314803576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83501.198013736267678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65080.9337333407940176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28740.41234628479440794No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.2886710942958764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.2747540484973177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.2251118026591601No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.16370463150675693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.15079190860087777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9490.13615748930754806No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.12912722905879165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70000.030.417091
GGTATCA47300.030.3447481
CGACTAC350.00838679527.14642388-89
GTACATG43400.024.4204041
TATCAAC88150.024.0395072
ATCAACG88300.023.8910523
TCAACGC88150.023.8778064
CAACGCA88850.023.6896865
ACATGGG41700.023.585623
AGTACTT58300.023.30160512-13
AACGCAG90700.023.1981686
TACATGG44850.022.670672
GAGTACT57250.022.27702312-13
CCTGTAC2457.481503E-921.3323943
ACGCAGA99650.021.0684877
CGCAGAG99650.021.020828
GTACTTT60750.020.87479414-15
CATGGGT6400.020.7869644
GGTACTC1856.546694E-620.5402938
AGAGTAC85400.020.41251810-11
Produced by FastQC (version 0.11.5)