##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937739_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 696987 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15544192359398 33.0 31.0 34.0 31.0 34.0 2 32.42550291468851 34.0 31.0 34.0 31.0 34.0 3 32.492069436015306 34.0 31.0 34.0 31.0 34.0 4 36.143336963243215 37.0 35.0 37.0 35.0 37.0 5 36.06834560759383 37.0 35.0 37.0 35.0 37.0 6 36.12365510404068 37.0 35.0 37.0 35.0 37.0 7 36.07137005424779 37.0 35.0 37.0 35.0 37.0 8 36.0563453837733 37.0 35.0 37.0 35.0 37.0 9 37.73728060925096 39.0 37.0 39.0 35.0 39.0 10-11 37.66829295237931 39.0 37.0 39.0 35.0 39.0 12-13 37.56606005563949 39.0 37.0 39.0 35.0 39.0 14-15 38.712613004259765 40.0 38.0 41.0 34.5 41.0 16-17 38.690036543005824 40.0 38.0 41.0 34.5 41.0 18-19 38.759980458746 40.0 38.0 41.0 35.0 41.0 20-21 38.71929749048404 40.0 38.0 41.0 34.5 41.0 22-23 38.639950960347896 40.0 38.0 41.0 34.0 41.0 24-25 38.65141817566181 40.0 38.0 41.0 34.0 41.0 26-27 38.50333937361816 40.0 38.0 41.0 34.0 41.0 28-29 38.34118642098059 40.0 38.0 41.0 34.0 41.0 30-31 38.26493607484788 40.0 37.5 41.0 34.0 41.0 32-33 38.084149345683635 40.0 37.0 41.0 34.0 41.0 34-35 37.920974135816024 40.0 37.0 41.0 33.5 41.0 36-37 37.75826808821398 40.0 36.0 41.0 33.0 41.0 38-39 37.61357744118614 40.0 36.0 41.0 33.0 41.0 40-41 37.42170585678068 40.0 36.0 41.0 33.0 41.0 42-43 37.166417738063984 40.0 36.0 41.0 33.0 41.0 44-45 36.793006182324774 40.0 35.0 41.0 31.5 41.0 46-47 36.30772238219651 39.5 35.0 41.0 30.5 41.0 48-49 35.89612144846317 39.0 35.0 41.0 29.5 41.0 50-51 35.62208620820761 39.0 35.0 41.0 28.0 41.0 52-53 35.411984728553044 39.0 35.0 40.5 27.0 41.0 54-55 35.2091645898704 39.0 35.0 40.0 27.0 41.0 56-57 34.96709838203582 38.5 35.0 40.0 26.0 41.0 58-59 34.75773507970737 38.0 35.0 40.0 26.0 41.0 60-61 34.47270106902998 38.0 34.0 40.0 24.5 41.0 62-63 34.12804256033469 38.0 34.0 40.0 23.0 41.0 64-65 33.766058764367195 37.5 34.0 40.0 22.0 41.0 66-67 33.372004786315955 37.0 33.0 39.5 21.5 41.0 68-69 32.96890114162818 36.0 33.0 39.0 19.5 40.5 70-71 32.58521177582939 36.0 32.0 39.0 18.0 40.0 72-73 32.09131160265543 35.5 32.0 38.5 15.0 40.0 74-75 31.613227362920686 35.0 31.0 38.0 10.0 39.5 76-77 30.312221748755718 34.0 29.5 36.0 8.0 39.0 78-79 30.880454728710866 35.0 31.0 37.0 7.0 39.0 80-81 30.64656083972872 35.0 31.0 36.0 2.0 39.0 82-83 30.246827415719373 34.0 31.0 36.0 2.0 37.5 84-85 29.817839500593266 34.0 30.0 35.5 2.0 37.0 86-87 29.429621355921988 34.0 30.0 35.0 2.0 36.5 88-89 28.97665953597413 34.0 29.0 35.0 2.0 36.0 90-91 28.41999850786313 34.0 29.0 35.0 2.0 36.0 92-93 27.96038448349826 34.0 28.5 35.0 2.0 35.0 94-95 27.49059666823054 33.0 27.0 35.0 2.0 35.0 96-97 26.96206600697 33.0 26.0 35.0 2.0 35.0 98-99 26.137917206490222 33.0 25.0 34.5 2.0 35.0 100-101 23.53196185868603 30.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20754452626228215 1101 2 0.19881907449258307 1101 3 0.17660468948185581 1101 4 0.11453370696193588 1101 5 -0.03406572172720246 1101 6 -0.002015475882750195 1101 7 0.10124114570707121 1101 8 -0.013919101457418037 1101 9 0.018743343768328202 1101 10-11 0.1824834377764546 1101 12-13 0.20948790411069496 1101 14-15 0.2671960268788567 1101 16-17 0.12140117332484834 1101 18-19 0.10540354906567728 1101 20-21 0.15725722427889366 1101 22-23 0.14571078623939115 1101 24-25 0.041519369999626576 1101 26-27 0.08447302436271542 1101 28-29 -0.11728212949246597 1101 30-31 0.019534455494529368 1101 32-33 0.22586797425349658 1101 34-35 0.18082812056321274 1101 36-37 0.20566921238827973 1101 38-39 0.23915589035828333 1101 40-41 0.20079998889176665 1101 42-43 0.23847307868737033 1101 44-45 0.1551276082915649 1101 46-47 0.14058226843901167 1101 48-49 0.2441740397333163 1101 50-51 0.42468955512455153 1101 52-53 0.3941535932618123 1101 54-55 0.2943030716327826 1101 56-57 0.3717584931058511 1101 58-59 0.2506165652390635 1101 60-61 0.6032572332779651 1101 62-63 0.5072511346982438 1101 64-65 0.5020893507279496 1101 66-67 0.5207930312955682 1101 68-69 0.5461645225695975 1101 70-71 0.44780899222379134 1101 72-73 0.3852023263241726 1101 74-75 0.5577012241982153 1101 76-77 0.63524849692309 1101 78-79 0.4982367649823267 1101 80-81 0.4986322559458394 1101 82-83 0.6574698323894275 1101 84-85 0.7238968336664513 1101 86-87 0.692153789738871 1101 88-89 0.6698925919002079 1101 90-91 0.7023851910921266 1101 92-93 0.5210584180714228 1101 94-95 0.49849702652873873 1101 96-97 0.6585306678532454 1101 98-99 0.24930279587360715 1101 100-101 0.46872036758905367 1102 1 0.28344356694355355 1102 2 0.137134538163302 1102 3 0.1552252199286812 1102 4 0.13451951383277816 1102 5 0.04995161783058677 1102 6 0.004405632549335792 1102 7 0.10709367396507474 1102 8 0.11083935884457219 1102 9 0.13565710768357775 1102 10-11 0.15166254480632801 1102 12-13 0.2425147171546982 1102 14-15 0.24767192420952 1102 16-17 0.30349004283356606 1102 18-19 0.21011421443273548 1102 20-21 0.24624429656125102 1102 22-23 0.23312250668604406 1102 24-25 0.17052721897105272 1102 26-27 0.21170031963109892 1102 28-29 0.21014523759657777 1102 30-31 0.3363233882738186 1102 32-33 0.3938492153827937 1102 34-35 0.29910989692736933 1102 36-37 0.3362823384677256 1102 38-39 0.2955864038759515 1102 40-41 0.3032598462764682 1102 42-43 0.4008753940484411 1102 44-45 0.40846855119414016 1102 46-47 0.2997355542339122 1102 48-49 0.4506605562606154 1102 50-51 0.6798110517301694 1102 52-53 0.7868757670335995 1102 54-55 0.6555374463655923 1102 56-57 0.7245090531359537 1102 58-59 0.5177124458115756 1102 60-61 0.7448294215848748 1102 62-63 0.7116578447993049 1102 64-65 0.7221704909663273 1102 66-67 0.6587805994824336 1102 68-69 0.7794341578232817 1102 70-71 0.6255757974017229 1102 72-73 0.6700225181879169 1102 74-75 0.7127205473740581 1102 76-77 0.689669742156422 1102 78-79 0.792889546322634 1102 80-81 0.9961377707460244 1102 82-83 0.7640593773542896 1102 84-85 0.8467037387092873 1102 86-87 0.6491490358956113 1102 88-89 0.6437592790870568 1102 90-91 0.948958747411492 1102 92-93 0.8405278002428034 1102 94-95 0.5897284942350964 1102 96-97 0.7660521712989272 1102 98-99 0.5049330221086699 1102 100-101 0.8884671997852251 1103 1 0.3074791280173912 1103 2 0.196605419947403 1103 3 0.245623411630568 1103 4 0.10707906748876894 1103 5 0.03509444313721133 1103 6 0.09732445811064849 1103 7 0.09999021416230391 1103 8 0.14963658590856 1103 9 0.10411695025339895 1103 10-11 0.21539510451916755 1103 12-13 0.24250755400195345 1103 14-15 0.305917110968835 1103 16-17 0.24669698631628734 1103 18-19 0.21900448289178343 1103 20-21 0.07043306951319295 1103 22-23 -0.0038063313709955082 1103 24-25 0.11779805777623409 1103 26-27 0.2182554002961652 1103 28-29 0.13317755933356068 1103 30-31 0.21080226891181297 1103 32-33 0.17914206894407414 1103 34-35 0.18125016737618438 1103 36-37 0.21407935063712813 1103 38-39 0.3080415346830776 1103 40-41 0.2592120282523851 1103 42-43 0.3525515717901442 1103 44-45 0.41031346097820887 1103 46-47 0.4911461381900395 1103 48-49 0.5757518864580788 1103 50-51 0.7024792465360932 1103 52-53 0.7982341395652952 1103 54-55 0.5511778720160478 1103 56-57 0.5137897930100408 1103 58-59 0.5229705978773538 1103 60-61 0.7315974866581385 1103 62-63 0.8420399592028147 1103 64-65 0.7228856123817664 1103 66-67 0.6708831439934713 1103 68-69 0.6268785041060028 1103 70-71 0.4398034105596409 1103 72-73 0.6647699374631593 1103 74-75 0.822790348318005 1103 76-77 0.7218334918281109 1103 78-79 0.8379859703176038 1103 80-81 0.8905019613495604 1103 82-83 0.7313424731097236 1103 84-85 0.6125163983879034 1103 86-87 0.35241941307539904 1103 88-89 0.5975454032642915 1103 90-91 0.6847044714072581 1103 92-93 0.6803191137262452 1103 94-95 0.6572706573480431 1103 96-97 0.6352717060123609 1103 98-99 0.6841980826373089 1103 100-101 0.8852246793105465 1104 1 0.2383679789779265 1104 2 0.127576765076455 1104 3 0.10935537195751266 1104 4 0.053233132460043464 1104 5 0.147768202405949 1104 6 0.08045203012710544 1104 7 0.10022252532651521 1104 8 0.13477910169623186 1104 9 0.12784777272973713 1104 10-11 0.2606014347613339 1104 12-13 0.22964130834001395 1104 14-15 0.10666145490313994 1104 16-17 0.22226496340756086 1104 18-19 0.3347357415441863 1104 20-21 0.23509000755722553 1104 22-23 0.28561829550884 1104 24-25 0.2678160737440791 1104 26-27 0.24401444146739237 1104 28-29 0.07315404375448509 1104 30-31 0.2925298105855987 1104 32-33 0.2511755976084302 1104 34-35 0.4282917558156285 1104 36-37 0.28180089963107946 1104 38-39 0.004343520232374942 1104 40-41 0.06687687435809408 1104 42-43 0.16016663693208955 1104 44-45 0.12463520719960286 1104 46-47 -0.0017953952532892004 1104 48-49 0.04149404951206748 1104 50-51 0.06434750240075715 1104 52-53 0.0712101878101592 1104 54-55 -0.07973942520949606 1104 56-57 0.30824751237815207 1104 58-59 0.32742054193303005 1104 60-61 0.1588840922601591 1104 62-63 0.25092040903307833 1104 64-65 0.37979378783833084 1104 66-67 0.22376332832526913 1104 68-69 0.32006135669745817 1104 70-71 0.25246816184445464 1104 72-73 0.30774133948474613 1104 74-75 0.19173129387185028 1104 76-77 0.3457871433803277 1104 78-79 0.32463329352406944 1104 80-81 0.545437862432415 1104 82-83 0.24620185373970926 1104 84-85 0.2837866189412779 1104 86-87 0.4060335110443347 1104 88-89 0.2138460613212203 1104 90-91 0.49139835166744916 1104 92-93 0.6681573872275983 1104 94-95 0.5595785976488159 1104 96-97 0.37233908573206875 1104 98-99 0.7983188085859929 1104 100-101 0.35791484259210904 1105 1 0.27506007031375646 1105 2 0.14902490984751182 1105 3 0.12771589087163449 1105 4 0.042294976641073845 1105 5 -0.005238132051474054 1105 6 0.13530804544944885 1105 7 0.07000118114789444 1105 8 0.030365485266202086 1105 9 0.14517345454024877 1105 10-11 0.18081344369615948 1105 12-13 0.12786905934228088 1105 14-15 0.18983075811282646 1105 16-17 0.12876839597912237 1105 18-19 0.303571827206504 1105 20-21 0.100548519996984 1105 22-23 0.06274598331125958 1105 24-25 0.147331123507918 1105 26-27 0.09362776457296462 1105 28-29 0.14848487804064803 1105 30-31 0.2821941036398883 1105 32-33 0.17475203323384392 1105 34-35 0.373998990991403 1105 36-37 0.169473432786873 1105 38-39 0.12529643876247576 1105 40-41 0.15092844253431537 1105 42-43 0.20246108065286705 1105 44-45 0.2740521765983388 1105 46-47 0.39654211119936633 1105 48-49 0.47657792197382776 1105 50-51 0.6216115040603611 1105 52-53 0.44634949280487746 1105 54-55 0.25160142456303447 1105 56-57 0.40547757427540176 1105 58-59 0.48059211261794843 1105 60-61 0.3649409045759242 1105 62-63 0.2007808628175951 1105 64-65 0.37210306663646975 1105 66-67 0.45134246079889095 1105 68-69 0.6060255643595909 1105 70-71 0.40838542794474364 1105 72-73 0.39524357077686645 1105 74-75 0.5383456397534907 1105 76-77 0.4930837822101175 1105 78-79 0.6274787337278518 1105 80-81 0.3817786157166374 1105 82-83 0.597943950141179 1105 84-85 0.8631012142451908 1105 86-87 0.5179641697217683 1105 88-89 0.5258542724762343 1105 90-91 0.6308414452755784 1105 92-93 0.8249412389443691 1105 94-95 0.6626763547780996 1105 96-97 0.7362857469953887 1105 98-99 1.0257586360648965 1105 100-101 0.668244760239336 1106 1 0.057709542763937804 1106 2 0.01984483478673127 1106 3 0.03537382142860679 1106 4 0.006704836826600058 1106 5 0.034624335197953826 1106 6 -0.05435339423834762 1106 7 -0.023716215800895668 1106 8 -0.16195219335720878 1106 9 -0.01801214386097172 1106 10-11 -0.10316178781993557 1106 12-13 -0.3477346134430732 1106 14-15 -0.1646300080736367 1106 16-17 0.07173951252789834 1106 18-19 0.09875582053444276 1106 20-21 0.261581431867441 1106 22-23 0.099639064973303 1106 24-25 -0.10607847703339957 1106 26-27 0.00791921126328532 1106 28-29 0.07536356125110899 1106 30-31 0.21581881547415804 1106 32-33 0.15330778199265893 1106 34-35 -0.34438617939027694 1106 36-37 -0.16798850573253077 1106 38-39 -0.07752738870788534 1106 40-41 -0.329056922973912 1106 42-43 -0.22742554137597892 1106 44-45 -0.03590557220769597 1106 46-47 0.27539133659414716 1106 48-49 -0.04715041150375043 1106 50-51 0.11574433174315146 1106 52-53 0.2788081661016477 1106 54-55 0.14624173865384904 1106 56-57 -0.06484194546148814 1106 58-59 -0.06379524506948542 1106 60-61 -0.016746310700597178 1106 62-63 -0.03907531002676734 1106 64-65 -0.2202869976451396 1106 66-67 -0.39955881932574044 1106 68-69 0.04725706470971858 1106 70-71 0.1975802948574099 1106 72-73 0.20427446843770625 1106 74-75 0.23355953150545972 1106 76-77 0.14836675505762997 1106 78-79 0.20381963870593722 1106 80-81 -0.19325147940756082 1106 82-83 -0.1048310956899563 1106 84-85 -0.5154828593364016 1106 86-87 -0.1846015871410991 1106 88-89 -0.11653618520569609 1106 90-91 -0.2136718005130689 1106 92-93 -0.4687360311721598 1106 94-95 -0.19903713963955383 1106 96-97 -0.05644264155823109 1106 98-99 -0.3522321898397287 1106 100-101 -0.5133897393947997 1107 1 0.14504951431442947 1107 2 0.06936797148754437 1107 3 0.09437694323639079 1107 4 0.061746031323814066 1107 5 0.021665491507917523 1107 6 -0.03724168698181529 1107 7 -0.02431575564451549 1107 8 0.12528276325252818 1107 9 0.11582844345895182 1107 10-11 0.0390370341033659 1107 12-13 -0.07492202692507988 1107 14-15 0.021083186576341006 1107 16-17 0.15491325497398378 1107 18-19 0.11088981959884592 1107 20-21 -0.0034314085957660723 1107 22-23 0.10353416459429354 1107 24-25 0.05749756810393336 1107 26-27 0.06195226232919282 1107 28-29 0.02240739066865416 1107 30-31 -0.010578121908302762 1107 32-33 0.0984995297276825 1107 34-35 0.10665871927030679 1107 36-37 0.014168501150727764 1107 38-39 0.14204262284376057 1107 40-41 0.08065600436528797 1107 42-43 0.17076056419267616 1107 44-45 0.2744107137002558 1107 46-47 0.009397083775446902 1107 48-49 -0.023180273934180207 1107 50-51 0.005065266948939495 1107 52-53 0.08879795101196208 1107 54-55 0.13805439199952474 1107 56-57 0.08022855775101334 1107 58-59 -0.05759459015812496 1107 60-61 0.09171312011842048 1107 62-63 -0.005661465238851804 1107 64-65 0.18249780764761425 1107 66-67 -0.11780850666762177 1107 68-69 0.04200918171545709 1107 70-71 -0.050658795522259936 1107 72-73 -0.02934940623173432 1107 74-75 -0.15935005063054675 1107 76-77 -0.38180594678586033 1107 78-79 -0.0513303894571564 1107 80-81 -0.123511057247498 1107 82-83 -0.4445868882186197 1107 84-85 -0.32669081755955176 1107 86-87 -0.2573864879019787 1107 88-89 -0.06842597679877116 1107 90-91 -0.10035495134316363 1107 92-93 -0.2841340855317078 1107 94-95 -0.15535856173278972 1107 96-97 -0.042801200753245894 1107 98-99 0.206051102283201 1107 100-101 0.4678383626033451 1108 1 0.10929182124494474 1108 2 0.0355571786827511 1108 3 0.027128982417480074 1108 4 -0.04816943044041011 1108 5 0.00846258173605463 1108 6 -0.004574276066570349 1108 7 -0.028072260317074438 1108 8 -0.05855188752082796 1108 9 0.12006660900624411 1108 10-11 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0.2025414745430396 2316 64-65 0.24513412817866964 2316 66-67 0.612727292289236 2316 68-69 0.3128958578652927 2316 70-71 0.14986277868352005 2316 72-73 0.2412053590598724 2316 74-75 0.12073529787585713 2316 76-77 0.3394363481406444 2316 78-79 0.36000199555943624 2316 80-81 0.12945243255809658 2316 82-83 0.06375829907393538 2316 84-85 0.05268329339350686 2316 86-87 -0.07236989235907032 2316 88-89 0.0732999596640056 2316 90-91 0.39348098986119595 2316 92-93 0.49433912452044737 2316 94-95 0.39329008691586154 2316 96-97 0.37526423310166024 2316 98-99 0.1341212777217926 2316 100-101 0.3742395005834318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 6.0 5 13.0 6 45.0 7 88.0 8 183.0 9 353.0 10 702.0 11 1091.0 12 1715.0 13 2491.0 14 3202.0 15 4620.0 16 6797.0 17 10277.0 18 9215.0 19 5095.0 20 3845.0 21 3762.0 22 3824.0 23 4249.0 24 4877.0 25 5635.0 26 6830.0 27 7919.0 28 9837.0 29 12056.0 30 14822.0 31 18379.0 32 23188.0 33 30545.0 34 42725.0 35 65487.0 36 89248.0 37 145960.0 38 142454.0 39 15447.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04957695707578 21.968957718667845 16.410277015624462 19.57118830863191 2 14.795397905556346 23.488960339288965 41.30937879759593 20.40626295755875 3 17.807936159497952 28.5507477183936 32.34020146717227 21.301114654936175 4 11.288732788416427 19.709693294136045 39.987689870829726 29.0138840466178 5 10.953699437480532 40.63517763357146 35.523412953019076 12.887709975928932 6 26.969369586520266 40.75829247891281 18.523014059085753 13.749323875481178 7 24.799888663633613 36.423921823506035 22.477320236962814 16.298869275897545 8 24.84924396007386 36.534971240496596 20.854621391790666 17.76116340763888 9 26.293890703843832 17.05426356589147 22.5807654949088 34.07108023535589 10-11 22.8226638373456 29.5503359460076 28.455050094191144 19.171950122455655 12-13 24.69359351459933 27.744386254394144 29.12662314369754 18.43539708730899 14-15 21.610589580580413 28.78037897406982 29.14566555760724 20.463365887742526 16-17 18.39431725412382 32.01099877042183 30.0407324670331 19.553951508421246 18-19 18.106578745371145 29.639505471407645 34.4577445490375 17.796171234183706 20-21 19.65086866756482 28.408636029079453 34.79447966748304 17.14601563587269 22-23 18.966558677268566 28.826138982068407 34.585398188188506 17.621904152474528 24-25 18.55480701563086 28.961751769941024 34.91803643255644 17.56540478187167 26-27 18.54849701785271 29.18300498865823 34.853532592710565 17.414965400778502 28-29 18.11093439620534 29.26219494260592 35.03783109833216 17.589039562856584 30-31 18.587434198916192 29.37271426870229 34.72202494451116 17.31782658787036 32-33 18.246107890104117 29.5024153965569 34.617431888973535 17.634044824365446 34-35 18.59841001338619 29.48778097726357 34.63371626730484 17.280092742045404 36-37 18.57753444468835 29.367405704840984 34.41362607910908 17.64143377136159 38-39 18.275663678088687 29.866267233104775 34.45595111530057 17.402117973505963 40-41 18.45114859659015 29.83467268117782 34.32410639601366 17.390072326218363 42-43 18.32246955211566 29.8781367150365 33.96423382540223 17.835159907445604 44-45 18.589371107352072 30.18227025755143 33.37429535988476 17.854063275211733 46-47 18.671438635153883 30.284926404653174 33.065609545084776 17.978025415108174 48-49 18.66785176768003 30.4374400096415 32.76273445559243 18.131973767086045 50-51 19.00250650299073 30.78730306304135 31.979577811350858 18.230612622617066 52-53 18.996193616236745 31.066146140458862 31.700160835137524 18.237499408166865 54-55 19.016064862041905 30.98716331868457 31.690117606210734 18.3066542130628 56-57 19.258537103274524 31.078700176617353 31.192690824936477 18.470071895171646 58-59 19.209181806834273 30.98465251145287 31.478205475855358 18.3279602058575 60-61 19.329054917810517 31.14921799115335 31.366438685370028 18.155288405666102 62-63 19.27769097558491 30.989171964469925 31.753389948449538 17.979747111495623 64-65 19.236872423732436 30.888094039056686 31.566370678362727 18.30866285884816 66-67 19.259541426167203 30.960333549980128 31.379997044421202 18.400127979431467 68-69 19.287232043065366 31.09857142242251 31.321459367247883 18.29273716726424 70-71 19.404307397412005 30.943762222250914 31.341402350402518 18.310528029934563 72-73 19.226255296009825 30.735939120815743 31.337241584132848 18.700563999041588 74-75 19.225466185165576 31.267082456344237 30.859972998061657 18.64747836042853 76-77 18.962261849934073 30.966000800588823 31.346782651613303 18.724954697863804 78-79 18.79009221118902 30.815782790783757 31.34907824679657 19.045046751230654 80-81 18.892820095640232 31.257756600912213 30.98881327772254 18.860610025725013 82-83 19.152961128065186 31.023012105064556 30.914687774846083 18.90933899202417 84-85 18.935947826823043 31.000170017640226 30.805187761886348 19.25869439365038 86-87 18.82976296544986 30.946703453579477 30.837160520927938 19.38637306004273 88-89 19.01692571023563 30.675751484604447 30.971452839149084 19.335869966010844 90-91 18.842082228735215 30.46057705760173 31.33093460659566 19.366406107067398 92-93 18.745310103489786 30.757255978317794 31.15755613169445 19.33987778649798 94-95 18.60608590978024 30.69799006294235 30.99756523435875 19.698358792918665 96-97 18.653289085736176 30.450854893993718 30.975254918671368 19.920601101598738 98-99 18.660963960176705 30.565196368900644 30.886439907084718 19.887399763837934 100-101 18.684056261466722 30.494214350649024 31.04239269803481 19.779336689849444 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2948.0 1 1922.0 2 1104.5 3 1323.5 4 1046.0 5 1003.0 6 1943.5 7 4040.5 8 5824.5 9 5925.0 10 4503.0 11 3060.5 12 2740.5 13 2633.0 14 2505.0 15 2342.5 16 2193.5 17 2188.5 18 2254.5 19 2386.5 20 2591.5 21 2913.0 22 3426.0 23 4061.5 24 4758.0 25 5783.0 26 7014.0 27 8257.0 28 9408.5 29 11165.0 30 13641.5 31 15726.5 32 18115.5 33 21149.0 34 23851.5 35 25865.5 36 28618.5 37 30530.0 38 30299.5 39 29855.0 40 28764.0 41 27216.5 42 25417.0 43 24683.0 44 24480.5 45 23608.5 46 22234.0 47 21533.0 48 22445.0 49 20752.0 50 17854.0 51 15464.5 52 12849.0 53 11108.5 54 9509.5 55 7946.5 56 6574.5 57 5569.5 58 4605.0 59 3792.5 60 3100.5 61 2478.5 62 1870.5 63 1154.0 64 712.0 65 494.0 66 351.5 67 264.5 68 187.5 69 128.0 70 101.0 71 84.5 72 65.0 73 43.0 74 26.5 75 15.5 76 15.5 77 12.5 78 7.0 79 4.0 80 2.5 81 3.5 82 4.0 83 5.0 84 3.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018795185563001894 2 0.0 3 0.0 4 0.0 5 0.043042409686263876 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005308563861305878 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.173734947710646E-4 24-25 7.173734947710645E-5 26-27 8.608481937252775E-4 28-29 0.0010760602421565969 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.608481937252775E-4 42-43 0.014275732545944185 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.608481937252775E-4 84-85 7.173734947710645E-5 86-87 0.0 88-89 0.0 90-91 0.012840985556402057 92-93 0.0014347469895421292 94-95 0.0 96-97 0.0 98-99 0.0011477975916337033 100-101 0.019153872310387423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 696987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.117437162386835 #Duplication Level Percentage of deduplicated Percentage of total 1 80.07456634072932 48.1387771029417 2 12.324653964020618 14.818532204603423 3 3.5860401953209093 6.467506383119945 4 1.4613554108831086 3.5141176834271706 5 0.7012141656497195 2.1077599270411262 6 0.4137914524878968 1.4925648985964346 7 0.26123179268584734 1.0993210117126364 8 0.1660822133310332 0.7987549618974811 9 0.1276056825606013 0.6904193942610386 >10 0.777139777688988 9.096599719600317 >50 0.0704812525338587 2.944171301074072 >100 0.033209650286969074 3.5143776794722035 >500 4.7783669475425265E-4 0.2655748050172816 >1k 0.0014335100842627578 1.5170368256314375 >5k 7.167550421313789E-4 3.534486101603713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9750 1.398878314803576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8350 1.198013736267678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6508 0.9337333407940176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2874 0.41234628479440794 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 0.2886710942958764 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1915 0.2747540484973177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.2251118026591601 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1141 0.16370463150675693 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.15079190860087777 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 949 0.13615748930754806 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.12912722905879165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.434746989542129E-4 0.0 10-11 0.0 0.0 0.0 1.434746989542129E-4 0.0 12-13 0.0 0.0 0.0 1.434746989542129E-4 0.0 14-15 0.0 0.0 0.0 1.434746989542129E-4 0.0 16-17 0.0 0.0 0.0 1.434746989542129E-4 0.0 18-19 0.0 0.0 0.0 1.434746989542129E-4 0.0 20-21 0.0 0.0 0.0 2.1521204843131936E-4 0.0 22-23 0.0 0.0 0.0 2.869493979084258E-4 0.0 24-25 0.0 0.0 0.0 6.456361452939581E-4 0.0 26-27 0.0 0.0 0.0 0.0010760602421565969 0.0 28-29 0.0 0.0 0.0 0.0019369084358818742 0.0 30-31 0.0 0.0 0.0 0.005452038560260091 0.0 32-33 0.0 0.0 0.0 0.010401915674180437 0.0 34-35 0.0 0.0 0.0 0.014634419293329717 0.0 36-37 0.0 0.0 0.0 0.020588619299929556 0.0 38-39 0.0 0.0 0.0 0.027618879548685988 0.0 40-41 0.0 0.0 0.0 0.03407524100162557 0.0 42-43 0.0 0.0 0.0 0.047490125353844476 0.0 44-45 0.0 0.0 0.0 0.06492230127678135 0.0 46-47 0.0 0.0 0.0 0.08013061936592791 0.0 48-49 0.0 0.0 0.0 0.10344525794598752 0.0 50-51 0.0 0.0 0.0 0.12805116881663503 0.0 52-53 0.0 0.0 0.0 0.17489565802518556 0.0 54-55 0.0 0.0 0.0 0.2177945930124952 0.0 56-57 0.0 0.0 0.0 0.2862320244136548 0.0 58-59 0.0 0.0 0.0 0.3689451883607585 0.0 60-61 0.0 0.0 0.0 0.4518018270068165 0.0 62-63 0.0 0.0 0.0 0.5667967982186182 0.0 64-65 0.0 0.0 0.0 0.6396102079378811 0.0 66-67 0.0 0.0 0.0 0.7851652900269301 0.0 68-69 0.0 0.0 0.0 0.9677368444461661 0.0 70-71 0.0 0.0 0.0 1.1053290807432563 0.0 72-73 0.0 0.0 0.0 1.3286474496654888 0.0 74-75 0.0 0.0 0.0 1.5586373920890921 0.0 76-77 0.0 0.0 0.0 1.7254267296233645 0.0 78-79 0.0 0.0 0.0 1.9156024430871739 0.0 80-81 0.0 0.0 0.0 2.233972800066572 0.0 82-83 0.0 0.0 0.0 2.5118832919408827 0.0 84-85 0.0 0.0 0.0 2.8735830080044535 0.0 86-87 0.0 0.0 0.0 3.3068048614966994 0.0 88-89 0.0 0.0 0.0 3.8915359970845946 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7000 0.0 30.41709 1 GGTATCA 4730 0.0 30.344748 1 CGACTAC 35 0.008386795 27.146423 88-89 GTACATG 4340 0.0 24.420404 1 TATCAAC 8815 0.0 24.039507 2 ATCAACG 8830 0.0 23.891052 3 TCAACGC 8815 0.0 23.877806 4 CAACGCA 8885 0.0 23.689686 5 ACATGGG 4170 0.0 23.58562 3 AGTACTT 5830 0.0 23.301605 12-13 AACGCAG 9070 0.0 23.198168 6 TACATGG 4485 0.0 22.67067 2 GAGTACT 5725 0.0 22.277023 12-13 CCTGTAC 245 7.481503E-9 21.332394 3 ACGCAGA 9965 0.0 21.068487 7 CGCAGAG 9965 0.0 21.02082 8 GTACTTT 6075 0.0 20.874794 14-15 CATGGGT 640 0.0 20.786964 4 GGTACTC 185 6.546694E-6 20.540293 8 AGAGTAC 8540 0.0 20.412518 10-11 >>END_MODULE