FastQCFastQC Report
Thu 26 May 2016
SRR937738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607904
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83641.3758751381797127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65811.0825722482497235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.8350002631994525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20760.34150128967731747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.22059404116439438No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.20430857503816394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.19049060377954413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8580.14114070642733062No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.13719271463915356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.11482076117281675No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.10198978786124124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.045.3843231
GTATCAA52150.035.5720861
GGTATAC1004.9024784E-528.5397591
ATCAACG66000.027.7355293
TATCAAC67200.027.3862862
TCAACGC67050.027.3034484
AGTACGC350.0083820127.14931730-31
CAACGCA67450.027.0005345
GTACATG37400.026.9626961
AACGCAG68200.026.8164736
ACATGGG37000.025.9578863
TACATGG38250.025.7353652
CATGGGT6400.024.5182534
ACGCAGA75100.024.3526427
CGCAGAG75550.024.207598
CATGGGA27300.024.0365414
GAGTACT47950.023.92115612-13
CCCTGTA2602.3646862E-1123.7772392
ATAGCCC2704.0017767E-1122.892823
GTACTTT50400.022.61696414-15
Produced by FastQC (version 0.11.5)