##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937738_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607904 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858707953887457 33.0 31.0 34.0 30.0 34.0 2 31.969998552403013 33.0 31.0 34.0 30.0 34.0 3 31.981506948465547 34.0 31.0 34.0 30.0 34.0 4 35.583067392219824 37.0 35.0 37.0 33.0 37.0 5 35.58541151234405 37.0 35.0 37.0 33.0 37.0 6 35.68367373795863 37.0 35.0 37.0 35.0 37.0 7 35.64332032689372 37.0 35.0 37.0 33.0 37.0 8 35.64117854134864 37.0 35.0 37.0 33.0 37.0 9 37.285790190556405 39.0 37.0 39.0 34.0 39.0 10-11 37.181579657314316 39.0 37.0 39.0 34.0 39.0 12-13 37.07718570695373 39.0 37.0 39.0 33.0 39.0 14-15 38.177839757593304 40.0 38.0 41.0 33.0 41.0 16-17 38.15956795809865 40.0 38.0 41.0 33.0 41.0 18-19 38.21502243775333 40.0 38.0 41.0 33.5 41.0 20-21 38.06831341790809 40.0 38.0 41.0 33.5 41.0 22-23 37.97628737432226 40.0 38.0 41.0 33.0 41.0 24-25 37.86543352239828 40.0 37.5 41.0 33.0 41.0 26-27 37.937172645680896 40.0 38.0 41.0 33.0 41.0 28-29 37.86913311970311 40.0 37.5 41.0 33.0 41.0 30-31 37.601676416013056 40.0 37.0 41.0 33.0 41.0 32-33 37.292398141811866 39.0 36.0 41.0 33.0 41.0 34-35 37.03414025898826 39.0 35.0 41.0 32.0 41.0 36-37 36.998370630889085 39.0 35.0 41.0 32.0 41.0 38-39 36.93385797757541 39.0 35.0 41.0 32.0 41.0 40-41 36.74205795651945 39.0 35.0 41.0 31.0 41.0 42-43 36.473463737695425 39.0 35.0 41.0 30.5 41.0 44-45 36.1523118781913 39.0 35.0 41.0 30.0 41.0 46-47 35.68610751039638 39.0 35.0 41.0 28.0 41.0 48-49 35.27694586645259 39.0 35.0 41.0 26.0 41.0 50-51 34.37329084855504 38.0 34.0 39.5 23.5 40.5 52-53 34.48723811654472 38.0 34.5 40.0 24.0 40.5 54-55 34.822979450702746 39.0 35.0 40.0 24.0 41.0 56-57 34.619025372427224 39.0 35.0 40.0 23.0 41.0 58-59 34.44290381376007 38.0 35.0 40.0 23.0 41.0 60-61 34.18078183397378 38.0 34.0 40.0 22.0 41.0 62-63 33.86126674606517 38.0 34.0 40.0 19.5 41.0 64-65 33.5442462954677 38.0 34.0 40.0 18.0 41.0 66-67 33.17177136521556 37.0 33.0 40.0 14.0 41.0 68-69 32.742593402905726 36.5 33.0 39.0 9.0 41.0 70-71 32.32049057087961 36.0 32.0 39.0 7.5 40.5 72-73 31.860562687529608 35.5 31.5 39.0 3.5 40.0 74-75 31.386839862873085 35.0 31.0 38.0 2.0 40.0 76-77 30.92784551508133 35.0 31.0 37.5 2.0 39.5 78-79 30.391445853292623 35.0 30.5 37.0 2.0 39.0 80-81 29.842576294941306 34.5 29.5 36.0 2.0 39.0 82-83 29.309519266199928 34.0 29.0 36.0 2.0 37.5 84-85 28.81375759988419 34.0 29.0 35.5 2.0 37.0 86-87 28.29976690398484 34.0 28.5 35.0 2.0 36.5 88-89 27.811730141601306 34.0 27.0 35.0 2.0 36.0 90-91 27.297212224298576 33.5 26.0 35.0 2.0 36.0 92-93 26.777154781018055 33.0 25.5 35.0 2.0 35.0 94-95 26.17961964389114 33.0 24.5 35.0 2.0 35.0 96-97 25.487307206401013 32.0 21.5 35.0 2.0 35.0 98-99 24.66438862057167 32.0 10.0 34.0 2.0 35.0 100-101 22.20654248039164 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2525326165984083 1101 2 0.09354785324373438 1101 3 0.13058198097090568 1101 4 0.11826828963738478 1101 5 0.13396560949841785 1101 6 0.1450670909281655 1101 7 0.16323890023916476 1101 8 0.07931707173505487 1101 9 0.057284834577281174 1101 10-11 0.16693953864073308 1101 12-13 0.24267290543656372 1101 14-15 0.40700628096653446 1101 16-17 0.4391697721730452 1101 18-19 0.34281464376989845 1101 20-21 0.46862907577733637 1101 22-23 0.331906493570024 1101 24-25 0.4001171143466493 1101 26-27 0.6342345012266435 1101 28-29 0.5209317662050807 1101 30-31 0.449427811947281 1101 32-33 0.49387973360819615 1101 34-35 0.6456623024773123 1101 36-37 0.6251359273985315 1101 38-39 0.548786639757175 1101 40-41 0.5864581954034307 1101 42-43 0.42801311412821974 1101 44-45 0.7597167241883653 1101 46-47 0.8540883314548608 1101 48-49 1.0599854709485967 1101 50-51 1.1850633451641812 1101 52-53 0.8524063657120351 1101 54-55 0.9838251255839907 1101 56-57 1.2536196848680632 1101 58-59 1.2283043190809053 1101 60-61 1.065102660554217 1101 62-63 1.0710146344844702 1101 64-65 1.2689905073506011 1101 66-67 1.4150139109086481 1101 68-69 1.5303974868843468 1101 70-71 1.551735355434971 1101 72-73 1.4282872342684847 1101 74-75 1.2869760937584687 1101 76-77 1.4343757274150555 1101 78-79 1.4828148461741044 1101 80-81 1.407025724518018 1101 82-83 1.82341749154034 1101 84-85 1.6039848445793758 1101 86-87 1.685263954383064 1101 88-89 1.7547062049706064 1101 90-91 1.8147889763877991 1101 92-93 1.9473794381130816 1101 94-95 1.9855600035708783 1101 96-97 2.0632446553797195 1101 98-99 2.2969128293238263 1101 100-101 2.3899895215160605 1102 1 0.10544071461094973 1102 2 0.11905385214748776 1102 3 0.06695502955086496 1102 4 0.05291982539734619 1102 5 -6.065981277458832E-5 1102 6 -0.04810370019630028 1102 7 0.06662711356059248 1102 8 -0.01886194123719065 1102 9 -0.07521481015768927 1102 10-11 -0.06980095548217236 1102 12-13 0.02850696621732851 1102 14-15 0.040269237989619455 1102 16-17 0.03775480231982442 1102 18-19 0.1604723306900553 1102 20-21 0.21671978001413095 1102 22-23 0.20011534880460147 1102 24-25 0.1780196296230443 1102 26-27 0.13202170762547638 1102 28-29 0.04185992146604178 1102 30-31 0.008675868140059606 1102 32-33 -0.003365439862648145 1102 34-35 0.05993177607604139 1102 36-37 0.23034918791945813 1102 38-39 0.22613900176208546 1102 40-41 0.4494943918345413 1102 42-43 0.6442436506799112 1102 44-45 0.5871249135547743 1102 46-47 0.6695352548597242 1102 48-49 0.6755481092481901 1102 50-51 0.5546039367312048 1102 52-53 0.5668240355794723 1102 54-55 0.6082064569143029 1102 56-57 0.8526452436492065 1102 58-59 0.8538549798738515 1102 60-61 0.8377472534204955 1102 62-63 0.8240201766189017 1102 64-65 0.8451024868873347 1102 66-67 0.8982514918583249 1102 68-69 0.8258419741861616 1102 70-71 1.0721377184549326 1102 72-73 0.838963455872662 1102 74-75 0.6864320307446015 1102 76-77 0.982273573493945 1102 78-79 0.9512896426579971 1102 80-81 1.0940433253625343 1102 82-83 1.147691228319406 1102 84-85 1.252006525896494 1102 86-87 1.064453544102708 1102 88-89 1.1863187021792392 1102 90-91 1.367589479849098 1102 92-93 1.5532808977448234 1102 94-95 1.5477151427332672 1102 96-97 1.5026587725968774 1102 98-99 1.7355352130506567 1102 100-101 1.7071640479985284 1103 1 0.11552469411737576 1103 2 0.15388005228177803 1103 3 0.09619700642660334 1103 4 0.12694872612124186 1103 5 0.09702627231375516 1103 6 0.15320060363842458 1103 7 0.08526357335988877 1103 8 0.12026073506918067 1103 9 0.13663890177328852 1103 10-11 0.3177212351595884 1103 12-13 0.1475987365936291 1103 14-15 0.38410230917443755 1103 16-17 0.1433077517205561 1103 18-19 0.09954303720329705 1103 20-21 0.24536019503661777 1103 22-23 0.21987812739488533 1103 24-25 0.16805568654147152 1103 26-27 -0.03551234868586306 1103 28-29 -0.11969622890602238 1103 30-31 -0.06579592559965874 1103 32-33 0.09340139513096801 1103 34-35 0.3488290966422909 1103 36-37 0.319473243954846 1103 38-39 0.4356894860469751 1103 40-41 0.3924849084970603 1103 42-43 0.45614979231369546 1103 44-45 0.2579330668334734 1103 46-47 0.34767252869913534 1103 48-49 0.3853101519829991 1103 50-51 0.2606632466827321 1103 52-53 0.2923573008461773 1103 54-55 0.3587422516842125 1103 56-57 0.6052041810738942 1103 58-59 0.46443382218922835 1103 60-61 0.5740842568330251 1103 62-63 0.6930498591892444 1103 64-65 0.7068016691679304 1103 66-67 1.0218522166759598 1103 68-69 1.0192321523551655 1103 70-71 1.1415390080416543 1103 72-73 1.259327796316196 1103 74-75 1.3013970316184356 1103 76-77 1.1418387748883916 1103 78-79 1.2185684491004132 1103 80-81 1.222975323118174 1103 82-83 1.1367173235942971 1103 84-85 1.1479423041096517 1103 86-87 1.3195245081653653 1103 88-89 1.0749226003736325 1103 90-91 1.3017925023754735 1103 92-93 1.2831101792739297 1103 94-95 0.9919032641862131 1103 96-97 1.1706329624659872 1103 98-99 1.6482282601362073 1103 100-101 1.8785797419562371 1104 1 -0.0026523181946345176 1104 2 0.03160233049762695 1104 3 -0.07495079697648777 1104 4 -0.13297696704687212 1104 5 0.019068326818612036 1104 6 0.028506921695381493 1104 7 0.1827344731444711 1104 8 0.10351452451998 1104 9 0.3472300956232104 1104 10-11 0.22829241952353385 1104 12-13 0.20772230134532066 1104 14-15 0.18854169772406948 1104 16-17 0.1643300635325744 1104 18-19 -0.013475974278691183 1104 20-21 0.3034766354231735 1104 22-23 0.4072941369599121 1104 24-25 0.034954315027071914 1104 26-27 0.2035392510848908 1104 28-29 0.1862957475371303 1104 30-31 0.10495786385114769 1104 32-33 0.3666247504006179 1104 34-35 0.20032165607787533 1104 36-37 0.13926064934209137 1104 38-39 0.2542997901911619 1104 40-41 0.3454786734424218 1104 42-43 0.2904144313524455 1104 44-45 0.26847386642241133 1104 46-47 0.4257712816882062 1104 48-49 0.4455930515304445 1104 50-51 0.30004240914987435 1104 52-53 0.44583005830156 1104 54-55 0.4741809287854508 1104 56-57 0.6038492177332486 1104 58-59 0.6647460689827724 1104 60-61 0.5569823220886505 1104 62-63 0.48230427115738905 1104 64-65 0.6643655018985086 1104 66-67 0.6751940478650695 1104 68-69 0.8885837956033242 1104 70-71 0.9498456620603477 1104 72-73 0.8424795620915795 1104 74-75 0.6490050334365733 1104 76-77 1.184423160572468 1104 78-79 1.1224068119786175 1104 80-81 0.8436942812415325 1104 82-83 0.7222714628405527 1104 84-85 0.6192163401958943 1104 86-87 0.49410233271428794 1104 88-89 0.829705114553196 1104 90-91 0.7125329027628133 1104 92-93 0.9460256848963482 1104 94-95 0.7584271471134265 1104 96-97 0.6199632622624343 1104 98-99 0.7239061123525659 1104 100-101 0.7190349701137855 1105 1 -0.30314475431607235 1105 2 -0.3320891857128174 1105 3 -0.28053821092150244 1105 4 -0.38719944811850837 1105 5 -0.2846628381530891 1105 6 -0.14919009008724515 1105 7 -0.04300026096242249 1105 8 -0.042355209512244585 1105 9 -0.2908816227884472 1105 10-11 -0.08981888821357842 1105 12-13 -0.010740693144178692 1105 14-15 -0.0954182876409817 1105 16-17 -0.17783277266053688 1105 18-19 -0.24258943992389703 1105 20-21 -0.04762397237516325 1105 22-23 0.07758127313959307 1105 24-25 0.06854655999817538 1105 26-27 -0.0942184658632712 1105 28-29 -0.21539623161553578 1105 30-31 -0.17750689280551057 1105 32-33 -0.017589293175674925 1105 34-35 -0.13350262001489455 1105 36-37 0.003742714064401298 1105 38-39 -0.01849264742364909 1105 40-41 0.18793924868545986 1105 42-43 -0.1864067236137288 1105 44-45 -0.0487815582150688 1105 46-47 -0.008847462991688815 1105 48-49 -0.04644172887066844 1105 50-51 -0.002021790482757524 1105 52-53 -0.17074364658024166 1105 54-55 -0.1346916949022514 1105 56-57 0.32740457666280776 1105 58-59 0.29080822270945106 1105 60-61 0.3759849802730244 1105 62-63 0.4022423339681964 1105 64-65 0.18037714265534532 1105 66-67 0.2361792187803573 1105 68-69 0.5288599555722584 1105 70-71 0.7340417852925825 1105 72-73 0.48199784084252784 1105 74-75 0.09784711787126099 1105 76-77 0.4296229350748071 1105 78-79 0.5088272979186144 1105 80-81 0.316119351802886 1105 82-83 0.6743998770394413 1105 84-85 0.8357454308877728 1105 86-87 0.5629584547518327 1105 88-89 0.35631682549804466 1105 90-91 0.3522910527859615 1105 92-93 0.7241895589760645 1105 94-95 1.076959396265945 1105 96-97 1.0920457929136909 1105 98-99 1.269440586614472 1105 100-101 1.1817065200742114 1106 1 0.02464194348959836 1106 2 0.10307842972045478 1106 3 0.024405106355779083 1106 4 0.05460953353866671 1106 5 0.1520130729061222 1106 6 0.01938221890252123 1106 7 0.17333474284886563 1106 8 0.03438946685292166 1106 9 0.2928855482652466 1106 10-11 -0.02615016183137442 1106 12-13 0.0041598079992084536 1106 14-15 -0.16748805847735326 1106 16-17 0.05431906243246942 1106 18-19 -0.011289062039075759 1106 20-21 0.03842996946625732 1106 22-23 -0.010483729536410635 1106 24-25 0.1305285901965263 1106 26-27 0.2345818714759602 1106 28-29 0.3222746680098254 1106 30-31 0.2040675732091799 1106 32-33 0.4940999979253746 1106 34-35 0.22196454617333217 1106 36-37 0.535879141836503 1106 38-39 0.30271350253013196 1106 40-41 0.047497464031160064 1106 42-43 -0.04533648244860444 1106 44-45 0.11234252748207041 1106 46-47 0.5345890183005793 1106 48-49 0.6773658255820365 1106 50-51 0.3681472035002784 1106 52-53 0.3509766213449552 1106 54-55 0.42105847944374375 1106 56-57 0.47440663637834035 1106 58-59 0.4590521124903475 1106 60-61 0.3097902641731807 1106 62-63 0.38262232715653965 1106 64-65 0.5805863578550756 1106 66-67 0.8259268630820813 1106 68-69 1.040614119603461 1106 70-71 0.6599001352496074 1106 72-73 0.5700182433790637 1106 74-75 0.7279578883994908 1106 76-77 0.7800462309440235 1106 78-79 0.5558723359503688 1106 80-81 0.5672384098479384 1106 82-83 0.6754946077356898 1106 84-85 0.8456061162702788 1106 86-87 0.2840900008359384 1106 88-89 0.7279981696651561 1106 90-91 0.8807743236791019 1106 92-93 0.6909665096239834 1106 94-95 0.5423131970087418 1106 96-97 0.24182927950932154 1106 98-99 0.6150795038852692 1106 100-101 0.4698112574199307 1107 1 -0.11882434180093426 1107 2 0.1044026524017383 1107 3 -0.22211218799243682 1107 4 -0.1695813664030652 1107 5 -0.12909291787728705 1107 6 0.07549394798422071 1107 7 0.18237476968644728 1107 8 0.2241858896860549 1107 9 0.20484239626213707 1107 10-11 -0.05387055662630047 1107 12-13 0.07017230866131996 1107 14-15 0.3783035618415127 1107 16-17 0.03967757365305147 1107 18-19 -0.0673522362891319 1107 20-21 0.237425325858716 1107 22-23 0.37399108374818724 1107 24-25 0.14810543925279518 1107 26-27 0.19308956079566286 1107 28-29 0.3720124719189286 1107 30-31 0.006109524583720827 1107 32-33 0.263804431422912 1107 34-35 0.04665511947347056 1107 36-37 0.19609684404530725 1107 38-39 0.30063447292051393 1107 40-41 0.2931540136365385 1107 42-43 0.348716874897292 1107 44-45 0.4998851233116497 1107 46-47 0.5541610295299861 1107 48-49 0.6978427223103267 1107 50-51 0.42332314855386954 1107 52-53 0.3978187916576559 1107 54-55 0.7993315165043242 1107 56-57 0.6708241726179054 1107 58-59 0.6738057187901134 1107 60-61 0.7205376554544998 1107 62-63 0.5190702322362597 1107 64-65 0.7081487287433603 1107 66-67 1.0133396850888943 1107 68-69 1.0917644277059395 1107 70-71 0.5395459498231148 1107 72-73 0.5538053436845196 1107 74-75 0.7282403892381666 1107 76-77 0.48371167932582537 1107 78-79 0.4853354077938654 1107 80-81 0.43357137239892296 1107 82-83 0.7699778960454537 1107 84-85 0.7604365274855631 1107 86-87 0.8329582318509985 1107 88-89 0.49891205987826837 1107 90-91 0.42300819906092 1107 92-93 0.04754436508948956 1107 94-95 0.35713831133329776 1107 96-97 0.665593779747681 1107 98-99 0.7713686788062581 1107 100-101 0.6503072229548295 1108 1 0.2183062000709839 1108 2 0.2561883666874536 1108 3 0.2172351026479582 1108 4 0.13676099812895615 1108 5 0.23328392941335352 1108 6 0.24575256867387196 1108 7 0.20669742978496686 1108 8 0.10411199806043925 1108 9 0.11063057360204454 1108 10-11 0.1981306749007956 1108 12-13 0.0717117175283093 1108 14-15 0.23282942391048067 1108 16-17 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-0.7054325787776357 2316 86-87 -0.5421297343699685 2316 88-89 -0.46286661710976773 2316 90-91 -0.316083989538658 2316 92-93 -0.5362434603855277 2316 94-95 -0.42386978155903066 2316 96-97 -0.5468886270081263 2316 98-99 -0.44093779550760814 2316 100-101 0.16398597031584572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2220.0 3 325.0 4 283.0 5 322.0 6 381.0 7 455.0 8 559.0 9 655.0 10 976.0 11 1293.0 12 2014.0 13 2971.0 14 3431.0 15 4537.0 16 6346.0 17 8996.0 18 7714.0 19 4534.0 20 4237.0 21 4179.0 22 4542.0 23 4796.0 24 5554.0 25 6194.0 26 6992.0 27 8057.0 28 9489.0 29 11081.0 30 13541.0 31 16851.0 32 21070.0 33 27665.0 34 38366.0 35 58250.0 36 77337.0 37 114435.0 38 110230.0 39 17024.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00603255997477 21.081436257765642 16.06239258526728 20.850138596992306 2 13.894393632135014 22.082096484902884 42.69416526550096 21.32934461746114 3 17.043347126800906 28.0414925983857 32.369770526857664 22.54538974795573 4 10.402043382272867 18.85558052582773 40.759404277506626 29.982971814392776 5 10.724863909601984 39.423230824825794 36.288242680654946 13.563662584917275 6 26.47523861183884 40.08481045424044 19.228594180987223 14.211356752933495 7 24.093545270807162 35.829071833879 23.133595122932306 16.943787772381526 8 23.906829062421693 35.67667462379489 21.74669955026839 18.669796763515027 9 25.521173390314516 16.63468070092133 22.77252372567253 35.07162218309163 10-11 22.08684240030073 28.682954013361382 29.271389095897277 19.95881449044061 12-13 23.90046863987272 27.197269770873433 29.594298833140297 19.307962756113543 14-15 21.02064722160626 28.026210686237572 29.578770959852214 21.374371132303953 16-17 17.867248227336155 31.352103830338574 30.434761107635705 20.345886834689566 18-19 17.46914363807511 29.06333817451669 34.80038773427673 18.667130453131463 20-21 19.279384264932887 27.88724710086461 34.860253886048795 17.97311474815371 22-23 18.343408400413445 28.35522812732335 34.69353607654303 18.607827395720168 24-25 17.852759674293893 28.545853626773248 35.0664047786054 18.534981920327454 26-27 17.821394542235527 28.64568050614716 35.03268921997554 18.50023573164178 28-29 17.444827225191315 28.68679274524691 35.3030741443284 18.565305885233375 30-31 17.94117841021126 28.742420195665293 35.05356559931151 18.262835794811934 32-33 17.44190456090829 28.975749461667068 34.770454178221996 18.81189179920264 34-35 17.829998615113727 29.033563708065973 34.820409926337234 18.31602775048306 36-37 17.842657772041438 28.925993783982257 34.51966798454155 18.711680459434756 38-39 17.549473496841312 29.419898836237657 34.516159337046076 18.514468329874955 40-41 17.76899515206752 29.28347343690642 34.577138821613715 18.37039258941234 42-43 17.56718109327674 29.24459646014052 34.19916345579296 18.989058990789772 44-45 17.846060267388008 29.57068078361946 33.70076312976338 18.882495819229156 46-47 17.901014776571124 29.733398611358375 33.22288818995246 19.14269842211804 48-49 17.902562195965412 29.891077200569466 33.04607105495931 19.160289548505805 50-51 18.189664126139405 30.094532013775886 32.43631783463327 19.279486025451433 52-53 17.9873098853437 30.527023182753315 32.25202122440229 19.233645707500692 54-55 18.14111551532146 30.172522508042558 32.35363693536196 19.332725041274017 56-57 18.23880506000328 30.347329291637447 31.802356291671234 19.61150935668804 58-59 18.28429267987181 30.220755045046754 32.14021723634691 19.35473503873452 60-61 18.49341874884133 30.383504644983212 32.02719014563207 19.09588646054339 62-63 18.3307926201208 30.27489143601111 32.35248069744638 19.041835246421716 64-65 18.356702131867262 30.091200938707168 32.35070666719403 19.201390262231538 66-67 18.195833686967024 30.266457150720466 32.03418614673312 19.503523015579383 68-69 18.429201680326045 30.104550690769578 32.154858294591016 19.311389334313358 70-71 18.46803566425499 29.95426603161 32.089918750103 19.487779554032006 72-73 18.216936091247835 29.823150463012553 32.19597651235112 19.763936933388486 74-75 18.19468265107566 30.0885400655541 31.938543650672642 19.7782336326976 76-77 18.068731705387517 29.971190105746153 32.194397563355395 19.76568062551093 78-79 17.859273837308965 29.744385985732556 32.09844798713029 20.297892189828186 80-81 17.925060836874767 30.14979097496397 31.827594930658737 20.09755325750253 82-83 18.063064473339473 29.875624308955278 31.973763781759217 20.087547435946025 84-85 18.066840562420392 29.60426906935013 31.81534314202054 20.513547226208935 86-87 17.80164586539761 29.84844789685822 31.90998164303111 20.43992459471306 88-89 18.29446952967931 29.346593718071258 31.812893444514025 20.546043307735406 90-91 17.932573418471485 29.2387584596356 32.12004050901606 20.708627612876857 92-93 17.863475761690683 29.38054541139866 32.16453968965472 20.591439137255936 94-95 17.66757578504157 29.335135090595983 32.08135922810082 20.915929896261627 96-97 17.530530514033323 29.239662475072926 32.01971092835836 21.210096082535394 98-99 17.786059298951468 29.19423014955361 31.993034511801856 21.02667603969306 100-101 17.759876210580437 28.830559112249254 32.49496981891348 20.91459485825683 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2231.0 1 1429.0 2 807.0 3 934.5 4 753.5 5 814.5 6 1582.0 7 3368.0 8 5062.5 9 5311.0 10 3990.0 11 2582.5 12 2297.5 13 2260.5 14 2132.0 15 2036.5 16 1941.5 17 1917.0 18 1992.5 19 2084.0 20 2278.0 21 2561.5 22 2984.5 23 3525.0 24 4148.0 25 4915.0 26 5970.5 27 7100.5 28 8178.5 29 9807.0 30 11818.5 31 13617.0 32 15784.5 33 18688.5 34 21040.0 35 22599.0 36 24877.5 37 26487.0 38 26239.5 39 25726.0 40 24735.0 41 23597.5 42 22211.5 43 21363.0 44 21116.0 45 20607.5 46 19569.5 47 18558.5 48 19321.5 49 18374.5 50 15737.0 51 13605.5 52 11556.0 53 9918.0 54 8555.5 55 7379.5 56 6256.5 57 5200.0 58 4194.5 59 3360.5 60 2673.0 61 2178.0 62 1652.0 63 1059.5 64 657.5 65 454.0 66 306.0 67 233.0 68 190.5 69 134.5 70 111.0 71 75.0 72 45.0 73 44.0 74 36.0 75 17.0 76 14.0 77 14.0 78 9.5 79 7.0 80 5.0 81 3.0 82 3.0 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3604187503289993 2 0.30613386324156444 3 0.2993893772700953 4 0.3043243670053166 5 0.308107859135653 6 0.22618702953097858 7 0.21549455177133234 8 0.21713954834973945 9 0.22832552508290782 10-11 0.2270095278201821 12-13 0.22396628415012895 14-15 0.2233082855187661 16-17 0.2073518187082171 18-19 0.21434305416644733 20-21 0.19978483444754433 22-23 0.21236905827235877 24-25 0.21113531083855347 26-27 0.2108885613517924 28-29 0.22659827867558036 30-31 0.2233905353476865 32-33 0.23062852029267777 34-35 0.22306153603200507 36-37 0.20488432384060642 38-39 0.1916421013844291 40-41 0.1892568563457388 42-43 0.20422632520924358 44-45 0.20685831973469496 46-47 0.20858556614202242 48-49 0.2259402800442175 50-51 0.21993604253303153 52-53 0.2511087276938464 54-55 0.2117933094699163 56-57 0.18843435805653524 58-59 0.18843435805653524 60-61 0.175438885087119 62-63 0.1840751171237564 64-65 0.1829236195188714 66-67 0.1823478707164289 68-69 0.18374611780807495 70-71 0.18654261199136707 72-73 0.18374611780807495 74-75 0.20225232931515505 76-77 0.19213560035795124 78-79 0.19871558667157974 80-81 0.1905728536084645 82-83 0.16992814654945518 84-85 0.1689411486024109 86-87 0.17272464073274726 88-89 0.17831762909933146 90-91 0.185144364899721 92-93 0.18029162499342002 94-95 0.17914012738853505 96-97 0.18654261199136707 98-99 0.19715283992209295 100-101 0.17552113491603938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 607904.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.7975818753308 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5107354046009 52.63985397568782 2 11.22944279688674 14.328225924974511 3 2.961744399399288 5.6685639244343555 4 1.1521888632478252 2.940274533555898 5 0.5548994377188045 1.7700621155220224 6 0.33374166066238153 1.2775146552790295 7 0.1896206224991123 0.8468136032396817 8 0.1428726031520472 0.7291941277867501 9 0.11500334865987899 0.660324199685926 >10 0.7198223036298962 8.9113007833209 >50 0.06209968298872149 2.7704714673738735 >100 0.024994453942448397 2.715069874391928 >500 0.0010306991316453864 0.49481474460584124 >1k 0.0010306991316453864 0.9562582622498931 >5k 7.730243487340398E-4 3.291257807891544 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8364 1.3758751381797127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6581 1.0825722482497235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.8350002631994525 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2076 0.34150128967731747 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1341 0.22059404116439438 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1242 0.20430857503816394 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.19049060377954413 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 858 0.14114070642733062 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.13719271463915356 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.11482076117281675 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.10198978786124124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.224982892035585E-5 0.0 22-23 0.0 0.0 0.0 6.579986313628467E-4 0.0 24-25 0.0 0.0 0.0 8.224982892035584E-4 0.0 26-27 0.0 0.0 0.0 0.0014804969205664052 0.0 28-29 0.0 0.0 0.0 0.003454492814654946 0.0 30-31 0.0 0.0 0.0 0.008471732378796653 0.0 32-33 0.0 0.0 0.0 0.013488971942938358 0.0 34-35 0.0 0.0 0.0 0.017930462704637575 0.0 36-37 0.0 0.0 0.0 0.023112201926619995 0.0 38-39 0.0 0.0 0.0 0.02911643943780597 0.0 40-41 0.0 0.0 0.0 0.0352029267779123 0.0 42-43 0.0 0.0 0.0 0.05206414170658525 0.0 44-45 0.0 0.0 0.0 0.07065260304258567 0.0 46-47 0.0 0.0 0.0 0.09047481181239143 0.0 48-49 0.0 0.0 0.0 0.11416276254145391 0.0 50-51 0.0 0.0 0.0 0.1422922040322156 0.0 52-53 0.0 0.0 0.0 0.2067760699057746 0.0 54-55 0.0 0.0 0.0 0.2552212191398642 0.0 56-57 0.0 0.0 0.0 0.3378000473759014 0.0 58-59 0.0 0.0 0.0 0.43370334789703635 0.0 60-61 0.0 0.0 0.0 0.5355286361004369 0.0 62-63 0.0 0.0 0.0 0.67954808653998 0.0 64-65 0.0 0.0 0.0 0.7658281570774332 0.0 66-67 0.0 0.0 0.0 0.9451327841238091 0.0 68-69 0.0 0.0 0.0 1.1546230983839554 0.0 70-71 0.0 0.0 0.0 1.3101575248723483 0.0 72-73 0.0 0.0 0.0 1.5772227193767439 0.0 74-75 0.0 0.0 0.0 1.8522661472864137 0.0 76-77 0.0 0.0 0.0 2.0495012370374273 0.0 78-79 0.0 0.0 0.0 2.2572643048902457 0.0 80-81 0.0 0.0 0.0 2.617189556245723 0.0 82-83 0.0 0.0 0.0 2.9099166973732693 0.0 84-85 0.0 0.0 0.0 3.3237978365005 0.0 86-87 0.0 0.0 0.0 3.8158985629309887 0.0 88-89 0.0 0.0 0.0 4.486892667263252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 45.384323 1 GTATCAA 5215 0.0 35.572086 1 GGTATAC 100 4.9024784E-5 28.539759 1 ATCAACG 6600 0.0 27.735529 3 TATCAAC 6720 0.0 27.386286 2 TCAACGC 6705 0.0 27.303448 4 AGTACGC 35 0.00838201 27.149317 30-31 CAACGCA 6745 0.0 27.000534 5 GTACATG 3740 0.0 26.962696 1 AACGCAG 6820 0.0 26.816473 6 ACATGGG 3700 0.0 25.957886 3 TACATGG 3825 0.0 25.735365 2 CATGGGT 640 0.0 24.518253 4 ACGCAGA 7510 0.0 24.352642 7 CGCAGAG 7555 0.0 24.20759 8 CATGGGA 2730 0.0 24.036541 4 GAGTACT 4795 0.0 23.921156 12-13 CCCTGTA 260 2.3646862E-11 23.777239 2 ATAGCCC 270 4.0017767E-11 22.89282 3 GTACTTT 5040 0.0 22.616964 14-15 >>END_MODULE