FastQCFastQC Report
Thu 26 May 2016
SRR937738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607904
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84981.397918092330368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74001.2172974680212665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57520.9462020318997736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24370.40088566615781435No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.29346738958782964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.269285939885245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.22552903089961573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.1702571458651366No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8560.1408117071116492No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.13258672421961362No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.12337474338053377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG250.00501276457.021181
GGTATCA44750.035.3595051
GTATCAA64050.027.5238861
GCTTCGT1109.466699E-525.910198
TAGGACG1151.2775559E-424.7918174
ACTATAC2751.8189894E-1224.1908043
ATCTCGG500.00165408423.74905448-49
GAGTACT49850.023.67856612-13
GTACATG41150.023.2103231
ATCAACG77750.022.6740173
TCAACGC78000.022.6013434
TATCAAC78850.022.4179652
CAACGCA78950.022.3293825
TACATGG41700.021.9925822
CTACGAT652.939164E-421.92581220-21
AACGCAG80600.021.9240076
AGTACTT51000.021.88728312-13
GTACTTT53250.021.8535914-15
ACATGGG40800.021.6624583
GTGTAGC3101.0913936E-1121.4595831
Produced by FastQC (version 0.11.5)