##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937738_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607904 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.270764791809235 33.0 31.0 34.0 31.0 34.0 2 32.4703801916092 34.0 31.0 34.0 31.0 34.0 3 32.520529557298524 34.0 31.0 34.0 31.0 34.0 4 36.14827670158446 37.0 35.0 37.0 35.0 37.0 5 36.078313352108225 37.0 35.0 37.0 35.0 37.0 6 36.133868176554195 37.0 35.0 37.0 35.0 37.0 7 36.08875579038796 37.0 35.0 37.0 35.0 37.0 8 36.071818905616674 37.0 35.0 37.0 35.0 37.0 9 37.754188161288624 39.0 37.0 39.0 35.0 39.0 10-11 37.6892979812602 39.0 37.0 39.0 35.0 39.0 12-13 37.60026747644365 39.0 37.0 39.0 35.0 39.0 14-15 38.755320741432854 40.0 38.0 41.0 35.0 41.0 16-17 38.72786657103753 40.0 38.0 41.0 34.5 41.0 18-19 38.80743176554193 40.0 38.0 41.0 35.0 41.0 20-21 38.75801360083171 40.0 38.0 41.0 35.0 41.0 22-23 38.601187029530976 40.0 38.0 41.0 34.0 41.0 24-25 38.650828091277575 40.0 38.0 41.0 34.0 41.0 26-27 38.50540381376007 40.0 38.0 41.0 34.0 41.0 28-29 38.36035130546928 40.0 38.0 41.0 34.0 41.0 30-31 38.288764344370165 40.0 37.5 41.0 34.0 41.0 32-33 38.092668414749696 40.0 37.0 41.0 34.0 41.0 34-35 37.94349190003685 40.0 37.0 41.0 33.5 41.0 36-37 37.75240662999421 40.0 36.0 41.0 33.0 41.0 38-39 37.60800471785019 40.0 36.0 41.0 33.0 41.0 40-41 37.40976042269832 40.0 36.0 41.0 33.0 41.0 42-43 37.14373980102121 40.0 36.0 41.0 33.0 41.0 44-45 36.75156850423751 40.0 35.0 41.0 31.5 41.0 46-47 36.28554508606622 40.0 35.0 41.0 30.5 41.0 48-49 35.85669283307891 39.0 35.0 41.0 29.5 41.0 50-51 35.58225229641522 39.0 35.0 41.0 27.5 41.0 52-53 35.36482405116597 39.0 35.0 41.0 27.0 41.0 54-55 35.158821129652054 39.0 35.0 40.0 26.5 41.0 56-57 34.92322636468916 39.0 35.0 40.0 26.0 41.0 58-59 34.70616742117176 38.0 35.0 40.0 25.0 41.0 60-61 34.39095811180712 38.0 34.0 40.0 24.5 41.0 62-63 34.08081703690056 38.0 34.0 40.0 23.0 41.0 64-65 33.72732865715639 37.5 34.0 40.0 22.0 41.0 66-67 33.33445659183029 37.0 33.0 39.5 20.5 41.0 68-69 32.91257418934569 36.0 33.0 39.0 18.5 40.5 70-71 32.51457055719324 36.0 32.5 39.0 16.5 40.0 72-73 32.01376779886298 35.0 32.0 38.5 10.5 40.0 74-75 31.50038986418908 35.0 31.0 38.0 7.5 40.0 76-77 30.200085046323103 34.0 29.5 36.0 7.0 39.0 78-79 30.77804719166184 35.0 31.0 37.0 2.0 39.0 80-81 30.546918592409327 35.0 31.0 36.0 2.0 39.0 82-83 30.14579851423909 34.0 30.5 36.0 2.0 37.5 84-85 29.703140627467494 34.0 30.0 35.5 2.0 37.0 86-87 29.252055423224718 34.0 29.5 35.0 2.0 36.5 88-89 28.8738040874875 34.0 29.0 35.0 2.0 36.0 90-91 28.346862333526346 34.0 29.0 35.0 2.0 36.0 92-93 27.872581855029743 34.0 28.5 35.0 2.0 35.0 94-95 27.392129349370954 33.5 27.0 35.0 2.0 35.0 96-97 26.876881876085697 33.0 26.0 35.0 2.0 35.0 98-99 26.055284222508817 33.0 24.5 35.0 2.0 35.0 100-101 23.536534551508133 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20816045642022374 1101 2 0.21456942390228306 1101 3 0.15618397418678143 1101 4 0.0753365217434876 1101 5 0.07128957347266862 1101 6 0.07590918786463163 1101 7 0.11503234455225453 1101 8 0.05629263803509588 1101 9 0.12733986564169442 1101 10-11 0.11601989867567397 1101 12-13 0.18492999782538533 1101 14-15 0.11848820430705587 1101 16-17 0.15485214994675545 1101 18-19 0.20121835762377316 1101 20-21 0.08679346051799541 1101 22-23 0.12817159996675542 1101 24-25 0.14495697233812166 1101 26-27 0.09463584405403935 1101 28-29 0.03991246691388284 1101 30-31 0.1918131377561494 1101 32-33 0.30038128696438093 1101 34-35 0.3600723874539611 1101 36-37 0.38318980303714056 1101 38-39 0.46126640772227745 1101 40-41 0.5028785679553494 1101 42-43 0.4843826570432057 1101 44-45 0.6392061722535871 1101 46-47 0.7529930348735263 1101 48-49 0.9531397232004934 1101 50-51 0.7591286879183414 1101 52-53 0.6321465339510581 1101 54-55 0.7742622471633354 1101 56-57 0.865447073264356 1101 58-59 0.9606361056991446 1101 60-61 0.9137212025673804 1101 62-63 1.1002924887090941 1101 64-65 1.238957868575234 1101 66-67 0.997571356487704 1101 68-69 0.8678347800933253 1101 70-71 0.960699428066512 1101 72-73 1.0112064901025555 1101 74-75 1.183337651271831 1101 76-77 1.017949435220583 1101 78-79 1.1517839040295108 1101 80-81 1.1787212357001486 1101 82-83 1.2444860442413592 1101 84-85 1.2579197449703905 1101 86-87 1.1563476048113763 1101 88-89 1.2672540012002145 1101 90-91 1.1947729628590302 1101 92-93 1.3834678337815092 1101 94-95 1.3063311674067073 1101 96-97 1.419691652383058 1101 98-99 1.482933475489201 1101 100-101 1.8403982336454554 1102 1 0.11229168117103683 1102 2 0.07930074203703441 1102 3 0.11637399022446004 1102 4 0.06500744482353582 1102 5 -0.03938664813150439 1102 6 0.054589226030245186 1102 7 0.011806538100636033 1102 8 -0.026693212266366118 1102 9 0.09323442298147455 1102 10-11 0.05674951153829966 1102 12-13 0.12073462236099886 1102 14-15 0.15610975569867946 1102 16-17 0.1289949461870279 1102 18-19 0.0963400612718388 1102 20-21 0.1072506358565164 1102 22-23 0.13484652231597494 1102 24-25 0.030740280972075595 1102 26-27 -0.024528166583614563 1102 28-29 0.02781518519886106 1102 30-31 0.0682113594009266 1102 32-33 0.006964855449332674 1102 34-35 -0.08587251601545631 1102 36-37 -0.0930155604497358 1102 38-39 0.1489722076776161 1102 40-41 0.0777867065696114 1102 42-43 0.21197355413815444 1102 44-45 0.2798667621965407 1102 46-47 0.2722159180014785 1102 48-49 0.3850648536335157 1102 50-51 0.2368099238346204 1102 52-53 0.25215303022584834 1102 54-55 0.3832920691248063 1102 56-57 0.17593043671062958 1102 58-59 0.2995102200741897 1102 60-61 0.32877225922707964 1102 62-63 0.6287847439314831 1102 64-65 0.7765063368754497 1102 66-67 0.7393302228877516 1102 68-69 0.7450267653143001 1102 70-71 0.5733143816790474 1102 72-73 0.6250191943799415 1102 74-75 0.5406611860573562 1102 76-77 0.3915623790481071 1102 78-79 0.7063465220282517 1102 80-81 0.504564228858758 1102 82-83 0.5566949806793708 1102 84-85 0.5067534606365633 1102 86-87 0.2514855491464232 1102 88-89 0.40209433494633373 1102 90-91 0.4392379337272949 1102 92-93 0.6273806212924242 1102 94-95 0.6771385325582528 1102 96-97 0.8958787450970824 1102 98-99 0.821896913642636 1102 100-101 1.2930383603228144 1103 1 0.07789411685266856 1103 2 0.1108579042103841 1103 3 -0.012873505567362997 1103 4 -0.008785982147408333 1103 5 0.019789929588633015 1103 6 -0.04160732096792685 1103 7 -0.026085419918807418 1103 8 0.009899960834431454 1103 9 -0.01500715837195088 1103 10-11 -0.08100631321771345 1103 12-13 -0.05209023483703845 1103 14-15 -0.04378768444408365 1103 16-17 0.14976584678215232 1103 18-19 0.08284585153462842 1103 20-21 0.04578692732887646 1103 22-23 0.22122997660554944 1103 24-25 0.0585689554204194 1103 26-27 -0.05487438658600041 1103 28-29 -0.13554059172654576 1103 30-31 0.0024148418369733804 1103 32-33 0.04731825004263612 1103 34-35 0.016238469387012344 1103 36-37 0.05532922463447676 1103 38-39 0.009427794200419726 1103 40-41 0.07651367659269681 1103 42-43 0.13522106832636638 1103 44-45 0.32587336649945087 1103 46-47 0.2774133572244182 1103 48-49 0.35463584988985275 1103 50-51 0.3492941719282996 1103 52-53 0.44760636319125524 1103 54-55 0.4337841724757041 1103 56-57 0.5167405216099539 1103 58-59 0.3636147299484449 1103 60-61 0.5080114766447821 1103 62-63 0.7590564585667323 1103 64-65 0.5159325535664792 1103 66-67 0.5424019595986636 1103 68-69 0.3729483415167394 1103 70-71 0.5530627191055757 1103 72-73 0.5591155178620149 1103 74-75 0.31708161708469973 1103 76-77 0.2569357797122187 1103 78-79 0.5239826782871617 1103 80-81 0.567030080565484 1103 82-83 0.46412298181994416 1103 84-85 0.3643957796851005 1103 86-87 0.5246286638386977 1103 88-89 0.530200794837608 1103 90-91 0.6816595421234091 1103 92-93 0.7440932789264636 1103 94-95 0.7358193276500948 1103 96-97 0.8032980079538561 1103 98-99 1.1626564084778508 1103 100-101 1.6762511973216299 1104 1 0.13608713675345996 1104 2 0.15549387978398954 1104 3 -0.08761264115756973 1104 4 0.003041847342132087 1104 5 0.0035081367386879947 1104 6 0.041918696304463765 1104 7 -0.016898348517052852 1104 8 -0.00494411339334988 1104 9 0.049606189190349426 1104 10-11 0.061048685695340055 1104 12-13 0.10361320975891175 1104 14-15 0.10120944999989945 1104 16-17 0.08875050123563 1104 18-19 0.03143382410434015 1104 20-21 -0.06952823442682643 1104 22-23 -0.047590056595375074 1104 24-25 -0.1179764685830591 1104 26-27 -0.19928235283172313 1104 28-29 -0.18935869808808548 1104 30-31 0.07916974786650144 1104 32-33 0.0032109201479642024 1104 34-35 0.1806163967006995 1104 36-37 0.10006401248452335 1104 38-39 0.08542678631520317 1104 40-41 -0.0723062577959439 1104 42-43 -0.29473584952111054 1104 44-45 -0.28080189683526413 1104 46-47 -0.22322768741896937 1104 48-49 -0.1847593695722196 1104 50-51 -0.23088581463323266 1104 52-53 -0.0781082965980815 1104 54-55 -0.3211650874203471 1104 56-57 -0.020259735301962678 1104 58-59 3.1039801825016866E-4 1104 60-61 -0.18150058833416693 1104 62-63 -0.05462311298323641 1104 64-65 -0.13916072522247447 1104 66-67 -0.12815049983890248 1104 68-69 0.1341795918872819 1104 70-71 0.10943018219919054 1104 72-73 0.08629753995643696 1104 74-75 0.023179322663640534 1104 76-77 0.10648914822272104 1104 78-79 0.2973295489023684 1104 80-81 0.1581510564355284 1104 82-83 0.38012143046720936 1104 84-85 0.3498370259055328 1104 86-87 0.3375967869983185 1104 88-89 0.4017880658401616 1104 90-91 0.4461207187799978 1104 92-93 0.39112965172542147 1104 94-95 0.46134599708350876 1104 96-97 0.6436099583105666 1104 98-99 0.8195060754804793 1104 100-101 0.559369860984404 1105 1 0.003806996974773824 1105 2 0.06048805567216675 1105 3 -0.09230739796356602 1105 4 -0.09074875343740274 1105 5 -0.08513596522806921 1105 6 0.0776473822952326 1105 7 0.017596447116353886 1105 8 0.004566735881624595 1105 9 -0.05233070133127882 1105 10-11 0.007707661547684097 1105 12-13 0.006959087333100911 1105 14-15 0.044905968973068866 1105 16-17 0.06311787575588568 1105 18-19 0.18615732812895658 1105 20-21 0.14427547060431323 1105 22-23 0.0319230700566564 1105 24-25 -0.06414263497345729 1105 26-27 -0.2157494690003574 1105 28-29 -0.11116626346000658 1105 30-31 -0.15988950873789065 1105 32-33 -0.13845499983769116 1105 34-35 -0.19025352400178264 1105 36-37 -0.2710271124650774 1105 38-39 -0.40111988871429105 1105 40-41 -0.14222080015515814 1105 42-43 -0.10243935742845878 1105 44-45 0.09157725284440943 1105 46-47 0.21309629000140262 1105 48-49 0.10310949084323084 1105 50-51 0.2964473768307059 1105 52-53 0.24348067791022743 1105 54-55 0.2162034967231392 1105 56-57 0.3095417968874514 1105 58-59 0.27017491275026373 1105 60-61 0.39565022629479785 1105 62-63 0.4407586650135684 1105 64-65 0.4245180974109246 1105 66-67 0.33966941563681274 1105 68-69 -0.18089645757287087 1105 70-71 0.06170606589616057 1105 72-73 -0.137759961947598 1105 74-75 -0.03836585900384648 1105 76-77 -0.05095974146262705 1105 78-79 0.2737026952667776 1105 80-81 0.29213642895468084 1105 82-83 0.2536999365078536 1105 84-85 0.41052072436587395 1105 86-87 0.48274410716019744 1105 88-89 0.29610563673751855 1105 90-91 0.04612743890902138 1105 92-93 0.3118423960767771 1105 94-95 0.36575402838437654 1105 96-97 0.3457831733696288 1105 98-99 0.4248627649955843 1105 100-101 0.7582884802218928 1106 1 0.046290657105494404 1106 2 -0.02837974951442135 1106 3 0.14192442545231643 1106 4 0.1802660213724323 1106 5 -0.009688747465858683 1106 6 0.0846455496349563 1106 7 -0.024035755806174564 1106 8 0.10884186197838375 1106 9 0.10792976348326278 1106 10-11 0.01931991604418215 1106 12-13 -0.03274720047565438 1106 14-15 0.044358603624942816 1106 16-17 0.32208250379071757 1106 18-19 0.11851108021705414 1106 20-21 0.06201254061842576 1106 22-23 0.10970571330419432 1106 24-25 0.24965989182717152 1106 26-27 0.23450921180860718 1106 28-29 0.08045215396486327 1106 30-31 0.08777101121864206 1106 32-33 0.03801964295573157 1106 34-35 0.17861801137829758 1106 36-37 0.04057378926864885 1106 38-39 0.11740538740112072 1106 40-41 0.013859415222967186 1106 42-43 -0.1237663332146397 1106 44-45 0.1010649188785635 1106 46-47 0.2547812021796716 1106 48-49 0.2887514895782104 1106 50-51 0.3379619975673407 1106 52-53 0.31520986586332356 1106 54-55 0.40039396364605295 1106 56-57 0.09075378540494938 1106 58-59 -0.09590708663343861 1106 60-61 -0.11725948680466303 1106 62-63 0.4298600127486907 1106 64-65 0.3261991172133847 1106 66-67 0.2143430237070092 1106 68-69 -0.013037750814731908 1106 70-71 -0.023869438085412753 1106 72-73 0.2721518108674026 1106 74-75 0.09117321882745344 1106 76-77 -0.19371667881159382 1106 78-79 -0.08011021120267614 1106 80-81 0.03549100817263806 1106 82-83 -0.15636185495811006 1106 84-85 -0.24083998534398177 1106 86-87 -0.07112069765337381 1106 88-89 -0.10512532711203804 1106 90-91 0.008951195376045717 1106 92-93 0.3116356136119336 1106 94-95 0.18246367336726266 1106 96-97 0.21134213475401964 1106 98-99 0.4709752546564623 1106 100-101 0.5590393843883561 1107 1 -0.017201406386568863 1107 2 0.025860411511054338 1107 3 -0.004569915702759886 1107 4 -0.07155699185213393 1107 5 -0.12368666402217343 1107 6 -0.0524821230878203 1107 7 -0.04063268007828924 1107 8 -0.14449076510285153 1107 9 0.016196444274974908 1107 10-11 0.056177447339983644 1107 12-13 -0.09188257134628941 1107 14-15 -0.061791086506943316 1107 16-17 0.11260371783807699 1107 18-19 -0.05632162631754767 1107 20-21 -0.2508218256086039 1107 22-23 -0.08898948594717382 1107 24-25 -0.08727974127486249 1107 26-27 0.16813034287134343 1107 28-29 0.10163530278983757 1107 30-31 -0.0112499468123346 1107 32-33 -0.013073282163887257 1107 34-35 0.05766792574522839 1107 36-37 -0.10586316890356784 1107 38-39 0.209134292533804 1107 40-41 0.10550003884357295 1107 42-43 0.20137447579713807 1107 44-45 0.09780733108259909 1107 46-47 -0.3345376176730781 1107 48-49 -0.657418858427512 1107 50-51 -0.5085738358984102 1107 52-53 -0.28524566673903706 1107 54-55 -0.36739917511482645 1107 56-57 -0.29278082977594266 1107 58-59 -0.1917499102293263 1107 60-61 -0.06628574172635382 1107 62-63 -0.2820288584838906 1107 64-65 -0.20668133196294747 1107 66-67 -0.23815269463965905 1107 68-69 -0.13575346668951482 1107 70-71 0.07124064896015625 1107 72-73 0.07482056097774858 1107 74-75 -0.04762738854934767 1107 76-77 -0.09378642120614344 1107 78-79 -0.25892441393233767 1107 80-81 -0.12345102618652604 1107 82-83 0.015561975753261237 1107 84-85 -0.134284200256495 1107 86-87 5.241634798096584E-4 1107 88-89 0.06432220718532733 1107 90-91 0.19057275019003228 1107 92-93 0.4403633004597509 1107 94-95 0.5304517377068301 1107 96-97 0.4076140064986369 1107 98-99 0.5151115966237256 1107 100-101 0.3158414748945617 1108 1 0.04221977587943826 1108 2 -0.041565696863330004 1108 3 0.05454338478985221 1108 4 0.034046456423723725 1108 5 -0.013157107371931431 1108 6 -0.015844192053336315 1108 7 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0.5000154252508153 2316 96-97 0.20454398538674567 2316 98-99 0.09757992878417454 2316 100-101 0.19963809699167712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 5.0 5 14.0 6 32.0 7 71.0 8 169.0 9 338.0 10 533.0 11 970.0 12 1550.0 13 2220.0 14 2858.0 15 4206.0 16 6040.0 17 9087.0 18 8393.0 19 4763.0 20 3527.0 21 3391.0 22 3527.0 23 3806.0 24 4298.0 25 4861.0 26 5774.0 27 7018.0 28 8466.0 29 10183.0 30 12635.0 31 15959.0 32 20221.0 33 26293.0 34 36785.0 35 56840.0 36 77996.0 37 125981.0 38 124628.0 39 14460.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24918761054667 21.816132614865698 16.426967216486364 19.50771255810127 2 14.893963520555879 23.4884126441017 41.22822024530189 20.389403590040533 3 17.834065905142918 28.595962520397954 32.42699505185029 21.142976522608834 4 11.419749532408954 19.772726898863798 39.94798512262647 28.859538446100775 5 11.062349539836667 40.55558572300685 35.47396082729025 12.908103909866236 6 26.98238537663842 40.62483550034216 18.512791493393692 13.879987629625731 7 24.854911301784494 36.57748591882929 22.45519029320419 16.11241248618203 8 24.906399694688634 36.50543506869506 20.880764068010738 17.707401168605568 9 26.253158393430542 17.21061220192662 22.48052324051166 34.05570616413118 10-11 22.851387719113543 29.53229457282729 28.3326808180239 19.283636890035268 12-13 24.80838925236028 27.753741324256875 29.10427013537367 18.33359928800917 14-15 21.723660314786546 28.886057667000053 29.12351292309312 20.26676909512028 16-17 18.42429067747539 31.99641719745223 30.061407722271937 19.51788440280044 18-19 18.093893114702322 29.542164552297734 34.58884955519292 17.77509277780702 20-21 19.76734813391588 28.30636087276939 34.82975930410065 17.096531689214086 22-23 19.038720311631227 28.659166504907073 34.71873570895117 17.583377474510524 24-25 18.579660604305943 28.898230641680268 35.05240959098805 17.46969916302574 26-27 18.590907744998372 29.04357939273107 34.93910202631345 17.426410835957114 28-29 18.167843119192106 29.20919689225713 35.10094571320001 17.522014275350756 30-31 18.665447175869872 29.31030228457125 34.74841422329841 17.275836316260463 32-33 18.251977285887243 29.498407643312103 34.65810391114387 17.59151115965679 34-35 18.63460349002474 29.353730194241194 34.69503408432911 17.316632231404956 36-37 18.573327367479074 29.424547296941622 34.473864294362265 17.528261041217032 38-39 18.332335368742434 29.788502789914194 34.569932094541244 17.309229746802128 40-41 18.55957979861786 29.76226313910699 34.35299284914105 17.32516421313409 42-43 18.34249625900902 29.822464373179375 34.0480771841094 17.786962183702197 44-45 18.707888087592778 29.995361109648893 33.51261054377007 17.784140258988263 46-47 18.788986418908248 30.228868373953784 33.07709769963679 17.905047507501184 48-49 18.790384665999895 30.37905656156235 32.73658340790651 18.093975364531243 50-51 19.066990840659052 30.792279702058217 31.98597146917934 18.154757988103384 52-53 19.08755329788914 30.964839843133124 31.781827393799023 18.165779465178712 54-55 19.056545112386168 30.887936253092597 31.69859056693162 18.35692806758962 56-57 19.27491840816971 31.10219705743012 31.15524819708375 18.467636337316417 58-59 19.277797152181925 30.887771753434752 31.472568037058483 18.36186305732484 60-61 19.38348818234458 31.098989314102226 31.369838000736962 18.147684502816233 62-63 19.315138574511764 30.897148233931677 31.7857753855872 18.001937805969366 64-65 19.229763252092436 30.862932305100806 31.652119413591617 18.25518502921514 66-67 19.22811825551403 30.992229036163604 31.366548007580143 18.413104700742224 68-69 19.283143391061746 31.045855924619676 31.422806890561667 18.248193793756908 70-71 19.49888469231984 30.807331420750643 31.362435516134124 18.33134837079539 72-73 19.331670790124754 30.536893983260516 31.316211112280882 18.81522411433384 74-75 19.293753618992472 31.097755566668422 30.820491393377903 18.787999420961203 76-77 19.14298968258146 30.768756250987 31.37765173448439 18.71060233194715 78-79 18.878145233457914 30.54890245828289 31.474624282781495 19.098328025477706 80-81 18.983934127702835 31.044976710941786 31.027046233489365 18.94404292786602 82-83 19.23635289869556 30.87999302500224 30.93024919989044 18.95340487641176 84-85 19.02109141036829 30.774393548954887 30.842003186371475 19.362511854305346 86-87 18.92346488919303 30.766042006632627 30.78027122703585 19.530221877138494 88-89 19.053830868031795 30.4414841817129 31.047171921882406 19.4575130283729 90-91 18.81837984396645 30.196456834118106 31.450861935645207 19.53430138627023 92-93 18.781399801936548 30.50563757020231 31.20618010613832 19.506782521722823 94-95 18.685269384639682 30.414588487655948 31.1069675475075 19.793174580196872 96-97 18.657304442806762 30.286361004369112 31.029488208664525 20.026846344159605 98-99 18.704951944628366 30.45982636732646 30.83176712987905 20.003454558166123 100-101 18.67015920443851 30.500171116800377 30.943100373166253 19.88656930559486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2402.0 1 1574.5 2 928.0 3 1046.0 4 820.0 5 879.0 6 1651.5 7 3411.5 8 4916.5 9 5045.0 10 3867.0 11 2619.5 12 2381.5 13 2297.0 14 2162.0 15 2056.5 16 1981.0 17 1983.5 18 2010.5 19 2140.5 20 2323.0 21 2555.0 22 3005.5 23 3631.5 24 4283.0 25 5070.0 26 6126.0 27 7172.0 28 8206.0 29 9792.5 30 11766.5 31 13471.5 32 15715.5 33 18595.0 34 21024.0 35 22625.5 36 24922.0 37 26577.5 38 26366.5 39 26036.5 40 25050.5 41 23521.5 42 21963.5 43 21407.5 44 21322.5 45 20671.0 46 19439.0 47 18643.5 48 19606.5 49 18326.0 50 15659.0 51 13586.0 52 11374.5 53 9791.5 54 8315.0 55 7072.5 56 5877.0 57 4883.0 58 4021.5 59 3324.5 60 2767.0 61 2207.0 62 1587.5 63 1037.5 64 642.0 65 398.5 66 297.5 67 237.5 68 184.5 69 124.5 70 85.0 71 71.0 72 48.0 73 36.0 74 29.0 75 12.5 76 9.5 77 9.5 78 8.0 79 6.0 80 5.0 81 3.0 82 2.0 83 1.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02122045586145181 2 0.0 3 0.0 4 1.644996578407117E-4 5 0.03043243670053166 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0050994893930620625 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.869979470442701E-4 24-25 0.0 26-27 3.289993156814234E-4 28-29 4.934989735221351E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.224982892035585E-4 42-43 0.01817721219139864 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.224982892035585E-5 82-83 0.0030432436700531663 84-85 4.1124914460177926E-4 86-87 0.0 88-89 0.0 90-91 0.014146970574301204 92-93 0.0029609938411328104 94-95 0.0 96-97 0.0 98-99 0.0018917460651681844 100-101 0.021713954834973945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 607904.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90310942893523 #Duplication Level Percentage of deduplicated Percentage of total 1 81.59711084800797 50.51114881909196 2 11.450778778864567 14.176776235891653 3 3.314525126086741 6.155382347553086 4 1.2814016296076955 3.1729098120008445 5 0.6523292159344062 2.0190603418839532 6 0.34724184739894426 1.2897210046705485 7 0.23582734021196172 1.0218911953233065 8 0.16440087284516305 0.8141540177557275 9 0.11333504816938586 0.6314212700066826 >10 0.7459263663297657 8.947174675091937 >50 0.06430756461077136 2.7828184818254496 >100 0.0299043217611971 3.1818835989144603 >500 7.939200467562199E-4 0.39513480103869225 >1k 0.001323200077927033 1.3538117727129986 >5k 7.939200467562199E-4 3.5467116262386758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8498 1.397918092330368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7400 1.2172974680212665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5752 0.9462020318997736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2437 0.40088566615781435 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.29346738958782964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1637 0.269285939885245 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.22552903089961573 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.1702571458651366 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 856 0.1408117071116492 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 806 0.13258672421961362 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.12337474338053377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.224982892035585E-5 0.0 22-23 0.0 0.0 0.0 6.579986313628467E-4 0.0 24-25 0.0 0.0 0.0 8.224982892035584E-4 0.0 26-27 0.0 0.0 0.0 0.0014804969205664052 0.0 28-29 0.0 0.0 0.0 0.003454492814654946 0.0 30-31 0.0 0.0 0.0 0.00888298152339843 0.0 32-33 0.0 0.0 0.0 0.01365347160077907 0.0 34-35 0.0 0.0 0.0 0.018094962362478286 0.0 36-37 0.0 0.0 0.0 0.023112201926619995 0.0 38-39 0.0 0.0 0.0 0.029034189608885614 0.0 40-41 0.0 0.0 0.0 0.0352029267779123 0.0 42-43 0.0 0.0 0.0 0.052146391535505605 0.0 44-45 0.0 0.0 0.0 0.07089935252934673 0.0 46-47 0.0 0.0 0.0 0.09080381112807286 0.0 48-49 0.0 0.0 0.0 0.11482076117281675 0.0 50-51 0.0 0.0 0.0 0.1431969521503395 0.0 52-53 0.0 0.0 0.0 0.20907906511554455 0.0 54-55 0.0 0.0 0.0 0.25875796178343946 0.0 56-57 0.0 0.0 0.0 0.3421592883086803 0.0 58-59 0.0 0.0 0.0 0.43896733694793916 0.0 60-61 0.0 0.0 0.0 0.5411216244670212 0.0 62-63 0.0 0.0 0.0 0.6848943254198031 0.0 64-65 0.0 0.0 0.0 0.7719146444175395 0.0 66-67 0.0 0.0 0.0 0.95228851923988 0.0 68-69 0.0 0.0 0.0 1.1631770805916724 0.0 70-71 0.0 0.0 0.0 1.3209322524609148 0.0 72-73 0.0 0.0 0.0 1.5883264462809916 0.0 74-75 0.0 0.0 0.0 1.8669888666631573 0.0 76-77 0.0 0.0 0.0 2.0660334526504185 0.0 78-79 0.0 0.0 0.0 2.275770516397326 0.0 80-81 0.0 0.0 0.0 2.640466257830184 0.0 82-83 0.0 0.0 0.0 2.9365656419434645 0.0 84-85 0.0 0.0 0.0 3.355217271148076 0.0 86-87 0.0 0.0 0.0 3.852828736116229 0.0 88-89 0.0 0.0 0.0 4.531307574880245 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 25 0.005012764 57.02118 1 GGTATCA 4475 0.0 35.359505 1 GTATCAA 6405 0.0 27.523886 1 GCTTCGT 110 9.466699E-5 25.91019 8 TAGGACG 115 1.2775559E-4 24.791817 4 ACTATAC 275 1.8189894E-12 24.190804 3 ATCTCGG 50 0.001654084 23.749054 48-49 GAGTACT 4985 0.0 23.678566 12-13 GTACATG 4115 0.0 23.210323 1 ATCAACG 7775 0.0 22.674017 3 TCAACGC 7800 0.0 22.601343 4 TATCAAC 7885 0.0 22.417965 2 CAACGCA 7895 0.0 22.329382 5 TACATGG 4170 0.0 21.992582 2 CTACGAT 65 2.939164E-4 21.925812 20-21 AACGCAG 8060 0.0 21.924007 6 AGTACTT 5100 0.0 21.887283 12-13 GTACTTT 5325 0.0 21.85359 14-15 ACATGGG 4080 0.0 21.662458 3 GTGTAGC 310 1.0913936E-11 21.459583 1 >>END_MODULE