FastQCFastQC Report
Thu 26 May 2016
SRR937737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555761
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80041.4401874186925674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60541.0893171705103453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.8541441374979533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18330.32981803329128884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.24740850833361822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.2184392211760091No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.18803046633354983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9030.16247991492745983No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.15204377421229628No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.1095794775092171No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.1056209413758792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGA405.380703E-447.5090267
GGTATCA32250.041.2667771
GTATCAA49950.034.0658951
CGGGTCG600.00394552631.6726866
CGTGGAC600.00394727131.6698283
AGTATCG650.005830920329.2416065
CCCTGTA1703.621608E-927.949012
TATCAAC61150.027.8164652
TCAACGC60750.027.767494
ATCAACG60750.027.759973
GTACATG33750.027.7437211
CAACGCA61600.027.4614775
GGACGTG700.008380108527.1480166
AACGCAG62600.027.0179146
TACATGG34350.026.6959842
ACATGGG34600.025.3999353
CATGGGA23700.024.8615344
GAGTACT42500.024.47670212-13
CGCAGAG68700.024.411488
ACGCAGA68650.024.2908527
Produced by FastQC (version 0.11.5)