##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 555761 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.909234365131773 33.0 31.0 34.0 30.0 34.0 2 32.03543969440101 33.0 31.0 34.0 30.0 34.0 3 32.01972790462087 34.0 31.0 34.0 30.0 34.0 4 35.62786161677412 37.0 35.0 37.0 35.0 37.0 5 35.63901389266249 37.0 35.0 37.0 35.0 37.0 6 35.70771788592578 37.0 35.0 37.0 35.0 37.0 7 35.672130646087076 37.0 35.0 37.0 33.0 37.0 8 35.66196080689361 37.0 35.0 37.0 33.0 37.0 9 37.318417449227276 39.0 37.0 39.0 34.0 39.0 10-11 37.22125248083259 39.0 37.0 39.0 34.0 39.0 12-13 37.12275690449672 39.0 37.0 39.0 34.0 39.0 14-15 38.255459631028444 40.0 38.0 41.0 33.5 41.0 16-17 38.22330822062001 40.0 38.0 41.0 33.5 41.0 18-19 38.268664048035035 40.0 38.0 41.0 33.5 41.0 20-21 38.25048357117538 40.0 38.0 41.0 34.0 41.0 22-23 38.074192863479084 40.0 38.0 41.0 33.5 41.0 24-25 37.93787347438917 40.0 38.0 41.0 33.0 41.0 26-27 37.87396110918182 40.0 37.5 41.0 33.0 41.0 28-29 37.87924485525252 40.0 38.0 41.0 33.0 41.0 30-31 37.64399714985399 40.0 37.0 41.0 33.0 41.0 32-33 37.33248014884096 39.5 36.0 41.0 33.0 41.0 34-35 37.28619586476921 40.0 36.0 41.0 33.0 41.0 36-37 37.18182006294072 40.0 36.0 41.0 33.0 41.0 38-39 37.03872617905898 40.0 35.5 41.0 32.0 41.0 40-41 36.79867065159304 39.5 35.0 41.0 31.0 41.0 42-43 36.525633320797965 39.0 35.0 41.0 31.0 41.0 44-45 36.20475798049881 39.0 35.0 41.0 30.0 41.0 46-47 35.753764657829535 39.0 35.0 41.0 28.5 41.0 48-49 35.326430785895376 39.0 35.0 41.0 26.5 41.0 50-51 34.38467974543014 38.0 34.0 40.0 23.5 40.5 52-53 34.488026327863956 38.0 34.5 40.0 24.0 40.5 54-55 34.85100609794498 39.0 35.0 40.0 24.0 41.0 56-57 34.68472688799682 39.0 35.0 40.0 23.0 41.0 58-59 34.4942709186143 38.5 35.0 40.0 22.5 41.0 60-61 34.237408706260425 38.0 34.5 40.0 22.0 41.0 62-63 33.931912278839285 38.0 34.0 40.0 19.5 41.0 64-65 33.598321940546384 38.0 34.0 40.0 18.0 41.0 66-67 33.231746200255145 37.0 33.5 40.0 13.5 41.0 68-69 32.79608230876222 37.0 33.0 39.5 8.0 41.0 70-71 32.389251854664145 36.0 32.5 39.0 7.0 41.0 72-73 31.944427550691756 36.0 32.0 39.0 2.0 40.0 74-75 31.488603914272502 35.0 31.5 38.5 2.0 40.0 76-77 31.003579776198762 35.0 31.0 37.5 2.0 39.5 78-79 30.47739855801325 35.0 30.5 37.0 2.0 39.0 80-81 29.928594305825705 35.0 30.0 36.5 2.0 39.0 82-83 29.38491455859623 34.0 29.0 36.0 2.0 37.5 84-85 28.90406307747395 34.0 29.0 35.5 2.0 37.0 86-87 28.43285513017286 34.0 29.0 35.0 2.0 37.0 88-89 27.96485089813787 34.0 27.5 35.0 2.0 36.0 90-91 27.48679828199532 34.0 26.5 35.0 2.0 36.0 92-93 26.96206102983117 33.0 26.0 35.0 2.0 35.5 94-95 26.386007654369415 33.0 25.0 35.0 2.0 35.0 96-97 25.735808198128332 33.0 22.5 35.0 2.0 35.0 98-99 24.905602408229434 32.0 10.5 34.5 2.0 35.0 100-101 22.645596758318774 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.062391076320388095 1101 2 0.0767014809875235 1101 3 0.020158761888243504 1101 4 -0.040876595770733104 1101 5 0.0652082736350863 1101 6 -6.966968855479649E-4 1101 7 -0.002895540242974448 1101 8 -0.0742112287679646 1101 9 0.02332692174296369 1101 10-11 -0.0910041255335372 1101 12-13 7.802475460252367E-4 1101 14-15 0.22145763607926483 1101 16-17 0.3110089662936133 1101 18-19 0.30211052620678913 1101 20-21 0.17345217652996325 1101 22-23 0.3404250565568532 1101 24-25 0.2609497025430869 1101 26-27 0.33302940329039643 1101 28-29 0.2975172670351469 1101 30-31 0.4075216453754038 1101 32-33 0.4699236499700419 1101 34-35 0.5546553066079909 1101 36-37 0.6159376972003159 1101 38-39 0.7580827316191616 1101 40-41 0.8232082906768383 1101 42-43 0.7161810741467818 1101 44-45 0.8629985879948379 1101 46-47 1.1362421161811511 1101 48-49 1.2283289478522974 1101 50-51 1.1707777846769432 1101 52-53 1.2487694441234325 1101 54-55 1.4252372429012468 1101 56-57 1.377117273456598 1101 58-59 1.4763985993761821 1101 60-61 1.5095235475289996 1101 62-63 1.4927560746982849 1101 64-65 1.6242872559838801 1101 66-67 1.641067189347794 1101 68-69 1.6091720046433764 1101 70-71 1.6762591723646096 1101 72-73 1.6595716580503677 1101 74-75 1.7200640604605333 1101 76-77 1.9176920572795133 1101 78-79 1.8488945463480455 1101 80-81 1.7799913633202493 1101 82-83 1.4797570019067763 1101 84-85 1.8690195211164529 1101 86-87 1.7991470857335052 1101 88-89 1.7519166074117827 1101 90-91 1.6493549374609522 1101 92-93 1.5459226332960974 1101 94-95 1.9493684757994068 1101 96-97 1.816347024725836 1101 98-99 1.9700296971244988 1101 100-101 2.680010986654473 1102 1 -5.125072654834639E-4 1102 2 -0.08632672894476912 1102 3 -0.07800107876447981 1102 4 -0.09754824373882798 1102 5 0.07912363772201303 1102 6 -0.11066283354989537 1102 7 -0.06872517157910352 1102 8 -0.06230197679905558 1102 9 0.014418683023777135 1102 10-11 -0.07948325197772022 1102 12-13 -0.021488820203074965 1102 14-15 0.14054240858736478 1102 16-17 -0.016621864224575233 1102 18-19 0.0815717301594816 1102 20-21 0.008535591242676333 1102 22-23 0.09732871220690242 1102 24-25 0.19154107748421723 1102 26-27 -0.10857367193270306 1102 28-29 -0.10710830967111917 1102 30-31 -0.018184928107629617 1102 32-33 -0.10162411445857344 1102 34-35 0.1881912219272195 1102 36-37 0.2512610706490932 1102 38-39 0.24646196020381694 1102 40-41 0.25454431241787745 1102 42-43 0.3413102787763336 1102 44-45 0.32001651456378966 1102 46-47 0.2815797403654017 1102 48-49 0.4785277242054562 1102 50-51 0.3966249287194614 1102 52-53 0.3016893158464242 1102 54-55 0.3885477152550294 1102 56-57 0.4542695116783264 1102 58-59 0.3499375931804778 1102 60-61 0.5752568487862817 1102 62-63 0.8192518412716439 1102 64-65 0.8972260471382327 1102 66-67 0.8438736056446032 1102 68-69 0.8550965797206942 1102 70-71 0.7001174315730552 1102 72-73 0.7419889212159916 1102 74-75 0.9284086917660694 1102 76-77 0.948286796128162 1102 78-79 1.0722269146816465 1102 80-81 0.8025203693773229 1102 82-83 0.590085013470464 1102 84-85 0.6672268642207477 1102 86-87 0.921896070954741 1102 88-89 1.028804422115524 1102 90-91 0.9610586852616692 1102 92-93 0.7840796401115782 1102 94-95 0.8815500615657932 1102 96-97 1.2721259120267767 1102 98-99 1.5209128757520674 1102 100-101 1.9806245429978837 1103 1 0.017595276982842023 1103 2 0.21011302753192496 1103 3 0.11953595728317978 1103 4 0.09461560785748446 1103 5 0.1836908445056551 1103 6 0.14468936189968673 1103 7 0.20223775293936086 1103 8 0.1900252582752202 1103 9 0.19413923672636457 1103 10-11 0.21289481464264526 1103 12-13 0.2966344831859544 1103 14-15 0.2889397392951736 1103 16-17 0.03965728186651063 1103 18-19 0.13487234609127796 1103 20-21 0.07885828760433355 1103 22-23 0.17456315045217252 1103 24-25 0.24212457173323543 1103 26-27 0.24763194904917896 1103 28-29 0.10534427426581061 1103 30-31 0.07258169200161291 1103 32-33 0.30102953139463295 1103 34-35 0.310291241516957 1103 36-37 0.34086566329709456 1103 38-39 0.5193711376130352 1103 40-41 0.4388512810208809 1103 42-43 0.4133508931307759 1103 44-45 0.3804781962789363 1103 46-47 0.7400005278727448 1103 48-49 0.7770619286459635 1103 50-51 0.7458189510517599 1103 52-53 0.7730393284398289 1103 54-55 0.8633971668696532 1103 56-57 0.9233361752920501 1103 58-59 1.0045228632262493 1103 60-61 0.9271171103728477 1103 62-63 0.8891299524141374 1103 64-65 0.9326701108125022 1103 66-67 0.9773375796131489 1103 68-69 0.9205180261554915 1103 70-71 1.1389071421283603 1103 72-73 1.0954049130544519 1103 74-75 1.0329383727586894 1103 76-77 1.03037478202355 1103 78-79 1.0575352494778478 1103 80-81 1.1359885411462223 1103 82-83 1.150665632862264 1103 84-85 1.0452464183380243 1103 86-87 1.1898696527978565 1103 88-89 0.8211207937913123 1103 90-91 0.8617705304366154 1103 92-93 0.831624817347798 1103 94-95 0.8456779494616278 1103 96-97 1.349973483535674 1103 98-99 1.5854858576913493 1103 100-101 1.8628883276837982 1104 1 0.030853925863386422 1104 2 0.09985093043134441 1104 3 0.1346779063099568 1104 4 0.11735551277510581 1104 5 -0.02545800096120132 1104 6 -0.008618824789856205 1104 7 0.06671569026701718 1104 8 -0.07061484792348693 1104 9 0.060084960987481395 1104 10-11 0.21475383154917438 1104 12-13 0.19532210898662328 1104 14-15 0.4032139672230528 1104 16-17 0.17980665077209323 1104 18-19 0.22019015973630474 1104 20-21 0.39571139558919555 1104 22-23 0.32942546448302323 1104 24-25 0.1788181658145973 1104 26-27 0.2496690430469073 1104 28-29 0.12704809428922914 1104 30-31 0.2613730881799725 1104 32-33 0.4890060899739055 1104 34-35 0.5611532965265909 1104 36-37 0.549984829248757 1104 38-39 0.6890192464882858 1104 40-41 0.5588995237237597 1104 42-43 0.6189058191208616 1104 44-45 1.1619479258715657 1104 46-47 0.8615759165394863 1104 48-49 1.129209376477469 1104 50-51 1.160859989726866 1104 52-53 1.2418029877621635 1104 54-55 1.27788508730697 1104 56-57 1.5046958208646757 1104 58-59 1.4964446388167332 1104 60-61 1.306499560605694 1104 62-63 1.2529495441319298 1104 64-65 1.1573386445904887 1104 66-67 0.9091907145793954 1104 68-69 1.256509025167972 1104 70-71 1.2693081393600707 1104 72-73 1.4070668040173047 1104 74-75 1.3248391028747655 1104 76-77 1.4628781681864176 1104 78-79 1.6114440416295075 1104 80-81 1.850795381465037 1104 82-83 2.1639053947785882 1104 84-85 2.24389141356232 1104 86-87 2.0304232027376834 1104 88-89 2.0186325711625663 1104 90-91 1.8494535316291945 1104 92-93 1.826924661395438 1104 94-95 1.9793625803294042 1104 96-97 2.2097485667732215 1104 98-99 2.4774848000213545 1104 100-101 2.6118347048942496 1105 1 0.17416882855832583 1105 2 0.02687140730597548 1105 3 -0.035095846719485735 1105 4 0.04361844687468874 1105 5 0.15812566978342346 1105 6 0.030294493160425873 1105 7 0.12591141784186277 1105 8 0.004524107480179396 1105 9 0.12553549576624334 1105 10-11 0.1607433536586811 1105 12-13 0.053426510571370045 1105 14-15 0.06913905465974324 1105 16-17 0.24884027580292667 1105 18-19 0.3981053207504246 1105 20-21 0.24242072845813567 1105 22-23 0.15319808555199899 1105 24-25 0.28203554576148093 1105 26-27 0.23674437646731405 1105 28-29 0.1654578349541822 1105 30-31 0.2795367000046056 1105 32-33 0.19492856519413237 1105 34-35 0.1413860740383157 1105 36-37 0.15227196988758607 1105 38-39 -0.030415644016301258 1105 40-41 0.015930014094841738 1105 42-43 0.19410282377931054 1105 44-45 0.29054444001382507 1105 46-47 0.32205163018251426 1105 48-49 0.3856245447477491 1105 50-51 0.20932531255328257 1105 52-53 0.16591374574030482 1105 54-55 0.23052415155297723 1105 56-57 0.1450400529455962 1105 58-59 0.27459635054413667 1105 60-61 0.36767767023171416 1105 62-63 0.507383781582412 1105 64-65 0.6239661280101672 1105 66-67 0.36708323638414697 1105 68-69 0.9061208207765432 1105 70-71 0.6900433331720208 1105 72-73 0.3897782847086688 1105 74-75 0.6263817618208805 1105 76-77 0.6806078618314793 1105 78-79 0.5242322924603364 1105 80-81 0.5414784760708073 1105 82-83 0.4844896605280802 1105 84-85 0.526084804654662 1105 86-87 0.5715235263622809 1105 88-89 0.3276026322594099 1105 90-91 0.5696590259911574 1105 92-93 0.8691337257865719 1105 94-95 0.8288190941814086 1105 96-97 1.0588538826337306 1105 98-99 0.8897186804689134 1105 100-101 0.9937436546381058 1106 1 0.005614829073831373 1106 2 0.13071194343450543 1106 3 0.2354987060771485 1106 4 0.21085790043599673 1106 5 0.15635146237729458 1106 6 0.23657939736181532 1106 7 0.2921842935184671 1106 8 0.2608004198781515 1106 9 0.35985545452558654 1106 10-11 0.06684782998826932 1106 12-13 0.3318503442340557 1106 14-15 0.11761400955278134 1106 16-17 0.26162702724205644 1106 18-19 0.29836951870557016 1106 20-21 0.35233996550599045 1106 22-23 0.5937084386751224 1106 24-25 0.5050315076138787 1106 26-27 0.7209741212907517 1106 28-29 0.5802572163443358 1106 30-31 0.7032457815408222 1106 32-33 0.31543934790742156 1106 34-35 0.08487778294800563 1106 36-37 -0.2435073066825666 1106 38-39 -0.24071506836547485 1106 40-41 0.02958453767934799 1106 42-43 -0.10690671312249123 1106 44-45 -0.08935503588616456 1106 46-47 -0.1576824535016499 1106 48-49 -0.09658827422295246 1106 50-51 -0.23367451561064456 1106 52-53 -0.14225491136081558 1106 54-55 0.026415121567580968 1106 56-57 -0.031899546676363855 1106 58-59 0.19402027012485945 1106 60-61 0.22089100471062295 1106 62-63 0.2740218089043438 1106 64-65 0.03105221788042911 1106 66-67 -0.11320931452229388 1106 68-69 0.07519892026962793 1106 70-71 0.09634327302939738 1106 72-73 -6.676298887988708E-4 1106 74-75 0.14997270606007618 1106 76-77 0.4113450385648747 1106 78-79 0.4997349129603812 1106 80-81 0.35665374886056966 1106 82-83 0.2857505076798965 1106 84-85 0.1442049180664675 1106 86-87 0.6060131732391589 1106 88-89 0.49547358337204983 1106 90-91 0.9439910132752907 1106 92-93 0.9267202882060204 1106 94-95 0.9359266635602204 1106 96-97 1.1701735836389702 1106 98-99 1.4748011617817376 1106 100-101 1.9695555800612539 1107 1 -0.0024710610479061756 1107 2 0.1680867244416504 1107 3 0.07107437653220572 1107 4 0.1626120893762959 1107 5 0.17987915655096032 1107 6 0.10342050962092486 1107 7 0.28157156273368855 1107 8 0.002882985800610527 1107 9 0.10190432923921122 1107 10-11 0.2566528701931077 1107 12-13 0.04940009491910757 1107 14-15 0.3175151820068436 1107 16-17 0.2587239680802895 1107 18-19 0.28158568150149677 1107 20-21 0.32960962994155807 1107 22-23 0.3518280200056978 1107 24-25 0.3381589502265214 1107 26-27 0.36815978231226154 1107 28-29 0.19576191065277726 1107 30-31 0.30757622491712766 1107 32-33 0.3841750291436625 1107 34-35 0.5644159887788334 1107 36-37 0.7716032004824314 1107 38-39 0.5136704039852731 1107 40-41 0.528309886944875 1107 42-43 0.1698777979057695 1107 44-45 0.05547224051650801 1107 46-47 0.24185597693723793 1107 48-49 0.2003379644904939 1107 50-51 -0.10853975077528588 1107 52-53 0.07128558547221076 1107 54-55 0.04814033606828616 1107 56-57 0.0053180086895210366 1107 58-59 0.10199273317682866 1107 60-61 0.24480053258447754 1107 62-63 0.27059653600554867 1107 64-65 0.19311254760506813 1107 66-67 0.1643332487048781 1107 68-69 0.09710943641895398 1107 70-71 0.5047986434080869 1107 72-73 0.6635770132089931 1107 74-75 0.5544188225867366 1107 76-77 0.4793338980051516 1107 78-79 0.43770378228341045 1107 80-81 0.20287022610776972 1107 82-83 0.6445113450187421 1107 84-85 0.8450045227562839 1107 86-87 0.875177855867971 1107 88-89 0.5757372732332406 1107 90-91 0.8312602284947346 1107 92-93 1.1278568049842441 1107 94-95 1.2848833591264572 1107 96-97 1.417096453231533 1107 98-99 1.3649958969688392 1107 100-101 1.8074615591277805 1108 1 0.004508634383565635 1108 2 -0.18820879531116574 1108 3 -0.17716000881319616 1108 4 -0.03777138005650471 1108 5 -0.18844461303633153 1108 6 -0.2770556545812397 1108 7 0.08522969559619042 1108 8 -0.0644448667705646 1108 9 0.08150320365987085 1108 10-11 0.10043045792208005 1108 12-13 0.1824419179162433 1108 14-15 0.22663430582056776 1108 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84-85 -0.8848721995062228 2316 86-87 -0.8593011080392579 2316 88-89 -0.9248415850800562 2316 90-91 -1.050026109176855 2316 92-93 -0.8243110512044503 2316 94-95 -0.8502450710861318 2316 96-97 -0.8292109396160718 2316 98-99 -1.117539756886611 2316 100-101 -0.7103272021154297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2286.0 3 297.0 4 261.0 5 298.0 6 318.0 7 387.0 8 486.0 9 658.0 10 818.0 11 1179.0 12 1787.0 13 2673.0 14 3129.0 15 4161.0 16 5750.0 17 8200.0 18 7235.0 19 4242.0 20 3762.0 21 3766.0 22 3944.0 23 4425.0 24 4875.0 25 5411.0 26 6322.0 27 7093.0 28 8221.0 29 9677.0 30 11670.0 31 14398.0 32 18550.0 33 24178.0 34 33897.0 35 51802.0 36 69926.0 37 105694.0 38 106504.0 39 17475.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.71655064691171 21.220509764619845 16.02986718672409 21.03307240174436 2 13.980261079448523 21.93377875583782 42.73582810855503 21.350132056158618 3 17.093219497667032 27.979747840762435 32.32241004304937 22.60462261852116 4 10.379433872030175 18.70081120345055 40.82780650226036 30.09194842225892 5 10.57873461580106 39.415851589850945 36.39152560724727 13.613888187100734 6 26.43975468975469 39.99007936507937 19.350649350649352 14.219516594516596 7 23.900520160737603 35.92662198526813 23.263665942818392 16.90919191117588 8 23.902334315209213 35.63325582947468 21.684906228748822 18.77950362656729 9 25.423783848139536 16.586603072782406 22.80883411412625 35.180778964951806 10-11 22.041705374617806 28.59118090067014 29.245262597746972 20.121851126965087 12-13 23.79912191144798 27.09889860133553 29.666604135025054 19.435375352191432 14-15 21.04365565369846 27.896112422822945 29.615426933642613 21.444804989835987 16-17 17.813933143637914 31.228495799494045 30.55289751486975 20.404673541998296 18-19 17.365738218837595 28.91142167596798 34.98517321366085 18.737666891533582 20-21 19.038447319111487 27.823043864822118 35.08076660192354 18.05774221414286 22-23 18.259228569908654 28.16443854968474 34.890355365648915 18.68597751475769 24-25 17.76750711392743 28.37230815838542 35.28949807590353 18.57068665178362 26-27 17.760986484000476 28.50777521669967 35.171177410569676 18.560060888730174 28-29 17.354868736504294 28.518263962925484 35.48975995006702 18.637107350503207 30-31 17.836959325513142 28.643414138753954 35.22052682560785 18.29909971012506 32-33 17.371084272145904 28.880099578774566 34.9735268384641 18.775289310615424 34-35 17.76579472360812 28.90552567307727 34.93598415567268 18.39269544764193 36-37 17.712066986413898 28.861200305500173 34.658129253264875 18.76860345482105 38-39 17.45246270594685 29.31316184320628 34.64875421992671 18.58562123092016 40-41 17.71984809085696 29.12205026850987 34.73226554526077 18.425836095372397 42-43 17.499172589303992 29.110698082120624 34.3479597900947 19.042169538480685 44-45 17.831358764362893 29.43727940526668 33.81175074440859 18.919611085961836 46-47 17.80575847724717 29.597570290160235 33.382629702806014 19.21404152978658 48-49 17.82029451871947 29.780427708713624 33.113153774558256 19.286123998008645 50-51 18.108519608249914 30.01381840879038 32.53089749302315 19.346764489936554 52-53 17.89773675564052 30.396687996694492 32.33542603971481 19.37014920795018 54-55 18.176636737815326 30.03755235144095 32.402971722105384 19.38283918863834 56-57 18.187440275498982 30.305609144835294 31.89549789950057 19.611452680165158 58-59 18.23722646819666 30.04968962017009 32.210268587181304 19.502815324451944 60-61 18.369131679733425 30.260752125902474 32.21220076885905 19.15791542550506 62-63 18.299478471626117 30.10077111180039 32.52492227568148 19.07482814089201 64-65 18.293917563599678 29.914635476626856 32.47890678597927 19.3125401737942 66-67 18.1931618090561 30.027417317538696 32.221073197295595 19.55834767610961 68-69 18.396946014274533 29.966152980593414 32.27972388622209 19.35717711890997 70-71 18.42479406347452 29.701920571654732 32.28215625481766 19.591129110053092 72-73 18.129223334670673 29.593523055631938 32.370296574450144 19.906957035247235 74-75 18.083855658057026 29.948500825862105 32.087898415354545 19.879745100726325 76-77 17.983821513599306 29.75687179857153 32.40666257845754 19.852644109371617 78-79 17.763145434687914 29.568085338566586 32.278566062443694 20.390203164301813 80-81 17.802647074205723 29.96082950555366 31.958398482233513 20.278124938007103 82-83 18.032624279750042 29.625875796896274 32.0391527425856 20.302347180768084 84-85 17.985843072870036 29.291369251284106 31.971556315304035 20.751231360541826 86-87 17.772139435366295 29.62933909670509 31.9939264593043 20.60459500862432 88-89 18.111580319921654 29.199185903175863 31.968088991567768 20.721144785334715 90-91 17.869583762039465 28.95396304816147 32.35607872983353 20.820374459965535 92-93 17.800187913314613 29.15154072687467 32.33903385144749 20.709237508363223 94-95 17.559966601564966 29.05681881709668 32.210283904731504 21.17293067660685 96-97 17.362676992845905 28.95645784403442 32.26185699924717 21.419008163872498 98-99 17.701958839573518 29.049162590446993 32.02632824651173 21.222550323467754 100-101 17.656153788593876 28.729790815273198 32.46559942157083 21.1484559745621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2046.0 1 1323.0 2 746.0 3 850.0 4 667.5 5 684.0 6 1382.5 7 2964.5 8 4628.5 9 4855.0 10 3513.0 11 2348.0 12 2147.0 13 2147.0 14 2052.5 15 1885.5 16 1768.5 17 1763.0 18 1826.0 19 1927.5 20 2082.5 21 2343.5 22 2735.5 23 3207.0 24 3754.5 25 4521.0 26 5538.5 27 6603.5 28 7548.5 29 8805.0 30 10715.0 31 12423.5 32 14457.0 33 17113.0 34 19218.0 35 20667.0 36 22525.5 37 24019.0 38 23985.0 39 23562.5 40 22557.0 41 21378.0 42 20219.5 43 19725.0 44 19490.5 45 18765.5 46 17912.0 47 17303.5 48 17990.0 49 17008.5 50 14532.5 51 12403.5 52 10543.5 53 9114.0 54 7766.5 55 6664.0 56 5609.0 57 4692.5 58 3787.5 59 3034.5 60 2482.5 61 1972.0 62 1454.5 63 916.0 64 572.5 65 404.0 66 270.5 67 204.5 68 158.0 69 116.0 70 103.0 71 81.5 72 51.0 73 40.5 74 37.0 75 23.0 76 15.5 77 11.0 78 5.5 79 6.0 80 4.5 81 1.5 82 1.5 83 2.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35554851815798516 2 0.3292782329094701 3 0.3141638222185436 4 0.2961704761579168 5 0.2916721396427601 6 0.24488943988513048 7 0.2369723676184547 8 0.2483081756366496 9 0.2537061794548376 10-11 0.25118711100634983 12-13 0.24740850833361822 14-15 0.24362990566088658 16-17 0.24533927353664614 18-19 0.24452957296391795 20-21 0.22707602728510995 22-23 0.2194288552093436 24-25 0.21861915463661538 26-27 0.235173033012392 28-29 0.25514564713968774 30-31 0.2490279094790746 32-33 0.2565851148245379 34-35 0.24596904064876807 36-37 0.2268061270942006 38-39 0.22473689229722849 40-41 0.21933888847904046 42-43 0.23760213473057665 44-45 0.23283389802451052 46-47 0.2323840643729949 48-49 0.24560917372755553 50-51 0.2569449817457504 52-53 0.27610789530031793 54-55 0.2402111699093675 56-57 0.20350474394568888 58-59 0.20116560895780738 60-61 0.21106194929115213 62-63 0.2225776907699533 64-65 0.20503417836084215 66-67 0.2131311840881242 68-69 0.21717968695176526 70-71 0.20881278103357376 72-73 0.21205158332448662 74-75 0.21394088466085243 76-77 0.2376921014608798 78-79 0.24425967277300853 80-81 0.22707602728510995 82-83 0.22779576112753502 84-85 0.21439071831236808 86-87 0.22032852251237492 88-89 0.23058472976693217 90-91 0.23076466322753844 92-93 0.22491682575783478 94-95 0.22383722499419714 96-97 0.21295125062751794 98-99 0.22014858905176865 100-101 0.20791311373054244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 555761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.35326234195814 #Duplication Level Percentage of deduplicated Percentage of total 1 83.08444647172459 53.46755180331271 2 10.761042116200795 13.8501633275346 3 2.9605298234838604 5.715592572055436 4 1.0961958323031586 2.8217511189746523 5 0.5256601648578997 1.6913973245908691 6 0.31669880054438987 1.2228360596889958 7 0.1958540063590185 0.8822690976361819 8 0.14011303673976747 0.7213384808674139 9 0.11117736279198348 0.6439163394815611 >10 0.7226758753447053 8.951658133607813 >50 0.05930908920509611 2.682695329461773 >100 0.023220062733040142 2.449010022420069 >500 0.0011190391678570907 0.5300217235906494 >1k 0.0013987989598213634 1.8388729228107654 >5k 5.595195839285453E-4 2.5309257439664905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8004 1.4401874186925674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6054 1.0893171705103453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4747 0.8541441374979533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1833 0.32981803329128884 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1375 0.24740850833361822 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.2184392211760091 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.18803046633354983 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 903 0.16247991492745983 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.15204377421229628 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.1095794775092171 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.1056209413758792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.799334606062678E-4 0.0 14-15 1.799334606062678E-4 0.0 0.0 1.799334606062678E-4 0.0 16-17 1.799334606062678E-4 0.0 0.0 1.799334606062678E-4 0.0 18-19 1.799334606062678E-4 0.0 0.0 1.799334606062678E-4 0.0 20-21 1.799334606062678E-4 0.0 0.0 2.699001909094017E-4 0.0 22-23 1.799334606062678E-4 0.0 0.0 6.297671121219372E-4 0.0 24-25 1.799334606062678E-4 0.0 0.0 0.0010796007636376067 0.0 26-27 1.799334606062678E-4 0.0 0.0 0.0018893013363658119 0.0 28-29 1.799334606062678E-4 0.0 0.0 0.0035087024818222217 0.0 30-31 1.799334606062678E-4 0.0 0.0 0.0065675713121287746 0.0 32-33 1.799334606062678E-4 0.0 0.0 0.011965575130316809 0.0 34-35 1.799334606062678E-4 0.0 0.0 0.017453545678807976 0.0 36-37 1.799334606062678E-4 0.0 0.0 0.02258164930608661 0.0 38-39 1.799334606062678E-4 0.0 0.0 0.030588688303065527 0.0 40-41 1.799334606062678E-4 0.0 0.0 0.03706629288489117 0.0 42-43 1.799334606062678E-4 0.0 0.0 0.05380010472127407 0.0 44-45 1.799334606062678E-4 0.0 0.0 0.07386268557887293 0.0 46-47 1.799334606062678E-4 0.0 0.0 0.09248579875162165 0.0 48-49 1.799334606062678E-4 1.799334606062678E-4 0.0 0.11812631688801481 0.0 50-51 1.799334606062678E-4 1.799334606062678E-4 0.0 0.15069427325774928 0.0 52-53 1.799334606062678E-4 1.799334606062678E-4 0.0 0.21376095120024616 0.0 54-55 1.799334606062678E-4 1.799334606062678E-4 0.0 0.2659416547760638 0.0 56-57 1.799334606062678E-4 1.799334606062678E-4 0.0 0.355458551427682 0.0 58-59 1.799334606062678E-4 1.799334606062678E-4 0.0 0.466297563161143 0.0 60-61 1.799334606062678E-4 1.799334606062678E-4 0.0 0.5713787041552034 0.0 62-63 1.799334606062678E-4 1.799334606062678E-4 0.0 0.7253117797038655 0.0 64-65 1.799334606062678E-4 1.799334606062678E-4 0.0 0.8073614377403235 0.0 66-67 1.799334606062678E-4 1.799334606062678E-4 0.0 0.9916133014011419 0.0 68-69 1.799334606062678E-4 1.799334606062678E-4 0.0 1.2176097279226141 0.0 70-71 1.799334606062678E-4 1.799334606062678E-4 0.0 1.3911555506773596 0.0 72-73 1.799334606062678E-4 1.799334606062678E-4 0.0 1.672301582874653 0.0 74-75 1.799334606062678E-4 1.799334606062678E-4 0.0 1.9509285466234587 0.0 76-77 1.799334606062678E-4 1.799334606062678E-4 0.0 2.1497550205933846 0.0 78-79 1.799334606062678E-4 1.799334606062678E-4 0.0 2.3788103159451635 0.0 80-81 1.799334606062678E-4 1.799334606062678E-4 0.0 2.750282945366803 0.0 82-83 1.799334606062678E-4 1.799334606062678E-4 0.0 3.0541005936004866 0.0 84-85 1.799334606062678E-4 1.799334606062678E-4 0.0 3.4914288696040203 0.0 86-87 1.799334606062678E-4 1.799334606062678E-4 0.0 4.017464341686444 0.0 88-89 1.799334606062678E-4 1.799334606062678E-4 0.0 4.710927898863 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 40 5.380703E-4 47.509026 7 GGTATCA 3225 0.0 41.266777 1 GTATCAA 4995 0.0 34.065895 1 CGGGTCG 60 0.003945526 31.672686 6 CGTGGAC 60 0.003947271 31.669828 3 AGTATCG 65 0.0058309203 29.241606 5 CCCTGTA 170 3.621608E-9 27.94901 2 TATCAAC 6115 0.0 27.816465 2 TCAACGC 6075 0.0 27.76749 4 ATCAACG 6075 0.0 27.75997 3 GTACATG 3375 0.0 27.743721 1 CAACGCA 6160 0.0 27.461477 5 GGACGTG 70 0.0083801085 27.148016 6 AACGCAG 6260 0.0 27.017914 6 TACATGG 3435 0.0 26.695984 2 ACATGGG 3460 0.0 25.399935 3 CATGGGA 2370 0.0 24.861534 4 GAGTACT 4250 0.0 24.476702 12-13 CGCAGAG 6870 0.0 24.41148 8 ACGCAGA 6865 0.0 24.290852 7 >>END_MODULE