FastQCFastQC Report
Thu 26 May 2016
SRR937737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555761
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75631.3608367625652034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66671.1996163818619874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50890.9156813810252968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22940.41276735863077835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.2697202574487954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.24668877449119317No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.22545662613965353No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8320.14970463922441482No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.14898490538198975No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.13153135970318178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.12109521898801823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.032.2246671
GTGTAGC2100.031.6856631
AGGGGTC1509.531504E-1031.6657055
GGGGTCC1401.704575E-830.5320426
GTATCAA58000.027.9434781
TAGGGCG705.811926E-727.13959330-31
GTACATG36050.025.5770551
TACATGG37200.024.3877012
TAACGCG801.86141E-623.74714376-77
CATGGGG7650.022.973164
GGTATAC1252.2447317E-422.8136771
ACATGGG37300.022.7942163
ATCAACG72450.022.2253
TATCAAC72800.022.1833932
TCAACGC72950.022.0726684
AGTACTT48150.021.9035912-13
GAGTACT47400.021.89937412-13
CAACGCA73850.021.803675
GTATAGT3500.021.7273121
GTATAGG3301.8189894E-1221.603861
Produced by FastQC (version 0.11.5)