##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937737_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 555761 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30077317408023 33.0 31.0 34.0 31.0 34.0 2 32.49521287028057 34.0 31.0 34.0 31.0 34.0 3 32.53747384217316 34.0 31.0 34.0 31.0 34.0 4 36.14456394025489 37.0 35.0 37.0 35.0 37.0 5 36.08639325177549 37.0 35.0 37.0 35.0 37.0 6 36.150503543789505 37.0 35.0 37.0 35.0 37.0 7 36.10230656703151 37.0 35.0 37.0 35.0 37.0 8 36.083042890739 37.0 35.0 37.0 35.0 37.0 9 37.76770950102652 39.0 37.0 39.0 35.0 39.0 10-11 37.704161501076904 39.0 37.0 39.0 35.0 39.0 12-13 37.60409510562994 39.0 37.0 39.0 35.0 39.0 14-15 38.77298335075689 40.0 38.0 41.0 35.0 41.0 16-17 38.74791772002713 40.0 38.0 41.0 34.5 41.0 18-19 38.81339010833794 40.0 38.0 41.0 35.0 41.0 20-21 38.76572483495603 40.0 38.0 41.0 35.0 41.0 22-23 38.701253776353504 40.0 38.0 41.0 34.5 41.0 24-25 38.705910454313994 40.0 38.0 41.0 35.0 41.0 26-27 38.523026084953784 40.0 38.0 41.0 34.0 41.0 28-29 38.45691853152704 40.0 38.0 41.0 34.0 41.0 30-31 38.371135254183 40.0 37.5 41.0 34.0 41.0 32-33 38.136940159529004 40.0 37.0 41.0 34.0 41.0 34-35 37.96170026324265 40.0 37.0 41.0 34.0 41.0 36-37 37.76758894560791 40.0 36.0 41.0 33.0 41.0 38-39 37.61166130764843 40.0 36.0 41.0 33.0 41.0 40-41 37.41046241100041 40.0 36.0 41.0 33.0 41.0 42-43 37.1471774737702 40.0 36.0 41.0 33.0 41.0 44-45 36.74662489811268 40.0 35.0 41.0 31.5 41.0 46-47 36.28587828221124 40.0 35.0 41.0 30.5 41.0 48-49 35.850736197754074 39.0 35.0 41.0 29.0 41.0 50-51 35.58036817984709 39.0 35.0 41.0 27.5 41.0 52-53 35.33357414428144 39.0 35.0 41.0 27.0 41.0 54-55 35.11586275395359 39.0 35.0 40.0 26.0 41.0 56-57 34.86420601661506 39.0 35.0 40.0 25.5 41.0 58-59 34.6472089621258 38.0 35.0 40.0 24.5 41.0 60-61 34.33373788373059 38.0 34.0 40.0 23.5 41.0 62-63 33.94837619048476 38.0 34.0 40.0 22.0 41.0 64-65 33.60679320787173 37.5 33.5 40.0 21.0 41.0 66-67 33.217176987949856 37.0 33.0 39.5 19.0 41.0 68-69 32.76720208866762 36.0 33.0 39.0 15.5 40.5 70-71 32.34548214070437 36.0 32.0 39.0 10.5 40.0 72-73 31.869868522620337 35.0 31.5 38.5 7.5 40.0 74-75 31.378094180771953 35.0 31.0 38.0 6.0 39.5 76-77 30.069397636753926 34.0 29.5 36.0 2.0 39.0 78-79 30.632090053098363 35.0 31.0 37.0 2.0 39.0 80-81 30.427413042656823 35.0 31.0 36.0 2.0 39.0 82-83 29.993219207537052 34.0 30.5 36.0 2.0 37.5 84-85 29.541785947556594 34.0 30.0 35.5 2.0 37.0 86-87 29.192676348286405 34.0 30.0 35.0 2.0 36.5 88-89 28.764986208100247 34.0 29.0 35.0 2.0 36.0 90-91 28.26315628480588 34.0 29.0 35.0 2.0 36.0 92-93 27.801373252171345 34.0 28.5 35.0 2.0 35.5 94-95 27.32220234957113 33.5 27.0 35.0 2.0 35.0 96-97 26.863202887572175 33.0 26.0 35.0 2.0 35.0 98-99 26.13629689740734 33.0 25.0 35.0 2.0 35.0 100-101 23.830959711098835 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12323565550921955 1101 2 0.14575691488742848 1101 3 0.19621444818552192 1101 4 0.09759267889585743 1101 5 0.15714141426807515 1101 6 0.14313451399819144 1101 7 0.0444318112954889 1101 8 0.18039825255097952 1101 9 0.14298540255058612 1101 10-11 0.2477532887658569 1101 12-13 0.1340289058085844 1101 14-15 0.1989578048235714 1101 16-17 0.2541677420122852 1101 18-19 0.21382381280479734 1101 20-21 0.17307479938430959 1101 22-23 0.07631037073681313 1101 24-25 0.06574200072617487 1101 26-27 0.11503257776457332 1101 28-29 0.19009728206291499 1101 30-31 0.22600402406292375 1101 32-33 0.18068320519221004 1101 34-35 0.2861484669687684 1101 36-37 0.3857207252076549 1101 38-39 0.4440920428372408 1101 40-41 0.43902253634151833 1101 42-43 0.34821807426633455 1101 44-45 0.5086273966315176 1101 46-47 0.6897240244503848 1101 48-49 0.6740275516526637 1101 50-51 0.6252032953347211 1101 52-53 0.7790034366704006 1101 54-55 0.7152117291989839 1101 56-57 0.8672566144753304 1101 58-59 0.8960697110138724 1101 60-61 1.0020313718625289 1101 62-63 0.8990197725071098 1101 64-65 1.001855197782703 1101 66-67 0.9526355039010639 1101 68-69 0.8779745768174863 1101 70-71 0.9054466277328572 1101 72-73 0.8193075653801181 1101 74-75 1.0578864981891378 1101 76-77 0.9583236305547231 1101 78-79 0.8019161881844958 1101 80-81 0.9071798349080602 1101 82-83 0.953300074836644 1101 84-85 1.1382060995545409 1101 86-87 1.0461381803395753 1101 88-89 1.217782380618445 1101 90-91 1.0423370914229366 1101 92-93 1.1505111494474782 1101 94-95 1.1347351525687692 1101 96-97 1.0938777887779914 1101 98-99 1.1538750903923543 1101 100-101 1.4083620332377045 1102 1 0.04352429916131939 1102 2 -0.04182476180640293 1102 3 -0.02092476253984188 1102 4 -0.001894376176409196 1102 5 -0.12425658492051639 1102 6 -0.12383334131708779 1102 7 -0.03389830423944318 1102 8 -0.13042985992993295 1102 9 -0.07646471070096794 1102 10-11 -0.016222727592925423 1102 12-13 -0.006498840312211485 1102 14-15 0.03251268034879473 1102 16-17 0.07137555606362866 1102 18-19 -0.006058001911171118 1102 20-21 -0.03455117895212112 1102 22-23 -0.029888189510764107 1102 24-25 0.0896573444257811 1102 26-27 -0.11782576503187414 1102 28-29 0.1235245329757575 1102 30-31 0.09516942841479903 1102 32-33 -0.18938790577391984 1102 34-35 -0.015363150744548193 1102 36-37 -0.04773506795077509 1102 38-39 0.0015164925325521494 1102 40-41 5.3770144821641E-4 1102 42-43 0.10183835230858307 1102 44-45 0.17920146273943516 1102 46-47 0.3589418242102269 1102 48-49 0.3237647591201309 1102 50-51 0.18947278714004057 1102 52-53 0.38589618510516743 1102 54-55 0.4745083463312625 1102 56-57 0.5105764038335536 1102 58-59 0.46723113576122444 1102 60-61 0.6180826133482782 1102 62-63 0.44079797479415816 1102 64-65 0.44555714742195107 1102 66-67 0.40791235348955013 1102 68-69 0.5406801574381177 1102 70-71 0.4644770884250917 1102 72-73 0.5249177476223572 1102 74-75 0.6104144237183391 1102 76-77 0.46728232788663604 1102 78-79 0.41063564033647637 1102 80-81 0.5014681238208247 1102 82-83 0.4994855056512577 1102 84-85 0.441484278396171 1102 86-87 0.38616806814816584 1102 88-89 0.33106757269756315 1102 90-91 0.5270037893501609 1102 92-93 0.6029612973574707 1102 94-95 0.6618304082359359 1102 96-97 0.7302526381666787 1102 98-99 0.4990079239645624 1102 100-101 0.9104812949721364 1103 1 0.038803681855696937 1103 2 0.20441566275658118 1103 3 0.12666735373668558 1103 4 0.050996636205830725 1103 5 0.13196697211741792 1103 6 0.20644195806239907 1103 7 0.17704497966160915 1103 8 0.11449143767375602 1103 9 0.21582812785162986 1103 10-11 0.2431568120737424 1103 12-13 0.2461540423045676 1103 14-15 0.24942447311484273 1103 16-17 0.2137515873868523 1103 18-19 0.19863373961626962 1103 20-21 0.13742570516060226 1103 22-23 0.305921846030607 1103 24-25 0.14156123769479478 1103 26-27 0.08336144923148225 1103 28-29 -0.029860547787379232 1103 30-31 0.1485120223501184 1103 32-33 0.2458241340263143 1103 34-35 0.19015161406414194 1103 36-37 0.13332878186465535 1103 38-39 0.08481548022663787 1103 40-41 0.12450447013414845 1103 42-43 0.19286447280325802 1103 44-45 0.3041311813850527 1103 46-47 0.18600135410746788 1103 48-49 0.26772094426093673 1103 50-51 0.3665658994401042 1103 52-53 0.24012311564811029 1103 54-55 0.5294060344881188 1103 56-57 0.5348104324752754 1103 58-59 0.5544735116653356 1103 60-61 0.5088297709286138 1103 62-63 0.7903736153403287 1103 64-65 0.5987774963227537 1103 66-67 0.852326480487001 1103 68-69 0.847604874341009 1103 70-71 0.7768926553952724 1103 72-73 1.019622158874494 1103 74-75 0.9764333570699897 1103 76-77 1.030887271623392 1103 78-79 0.9886078737952353 1103 80-81 1.202547848953948 1103 82-83 0.9927855618763424 1103 84-85 1.281640309363219 1103 86-87 1.4846891703633318 1103 88-89 1.4892686753197886 1103 90-91 1.4392824825876325 1103 92-93 1.627332072525828 1103 94-95 1.736574787635675 1103 96-97 1.7993853217314033 1103 98-99 1.8175611838745525 1103 100-101 2.0037983968107724 1104 1 0.15908126626693786 1104 2 0.08392222732571497 1104 3 0.0679396875718723 1104 4 0.176006754222378 1104 5 0.01977840466667402 1104 6 0.06826492784865223 1104 7 0.0865332508568315 1104 8 0.10547965760436284 1104 9 0.13883406915094554 1104 10-11 0.07493601806577033 1104 12-13 0.14997622423459944 1104 14-15 0.2446433730301223 1104 16-17 0.20749190781769045 1104 18-19 0.1794931504509094 1104 20-21 0.07386297615615689 1104 22-23 0.17910127758694472 1104 24-25 0.26173899100945874 1104 26-27 0.275703594682696 1104 28-29 0.3315280218218746 1104 30-31 0.4415432721814838 1104 32-33 0.4111795057487555 1104 34-35 0.44812326203271624 1104 36-37 0.3546246434609799 1104 38-39 0.31580575074193007 1104 40-41 0.5665484713645057 1104 42-43 0.7826342137280733 1104 44-45 0.8589141701661944 1104 46-47 0.7247681972323434 1104 48-49 0.5706867372705986 1104 50-51 0.708573745624669 1104 52-53 0.9639417360363112 1104 54-55 0.8777868820271522 1104 56-57 0.9659134851483202 1104 58-59 0.7960809542084988 1104 60-61 0.9049730528354445 1104 62-63 0.9843967006068794 1104 64-65 1.2059826033578815 1104 66-67 1.1202650492868145 1104 68-69 1.282307242766322 1104 70-71 1.1923707923851197 1104 72-73 0.9865025180505889 1104 74-75 1.3666866042787653 1104 76-77 1.3492675255721167 1104 78-79 1.2064528172152897 1104 80-81 1.3843294832699407 1104 82-83 1.4384828416138653 1104 84-85 1.2433429422060307 1104 86-87 1.246222360155734 1104 88-89 1.3273480329471568 1104 90-91 1.303733438537833 1104 92-93 1.2680075782917477 1104 94-95 1.6363632536364392 1104 96-97 1.7265409988830278 1104 98-99 1.5581919005539007 1104 100-101 1.9772244381570339 1105 1 -0.028400411937013814 1105 2 -0.06465774277891967 1105 3 -0.11935042809126628 1105 4 -0.019294158602150446 1105 5 0.04510239206998534 1105 6 0.04406419352557833 1105 7 0.015298107837985242 1105 8 0.09189613198287105 1105 9 0.09379945728277761 1105 10-11 0.16920800784910028 1105 12-13 -0.03333026131839034 1105 14-15 -0.1895752114388003 1105 16-17 -0.05997874102784806 1105 18-19 -0.09674438709970445 1105 20-21 0.015426242587729178 1105 22-23 -0.1499378621230889 1105 24-25 -0.06826666879288723 1105 26-27 -0.01982806392962999 1105 28-29 -0.11677186062838274 1105 30-31 -0.11916173601797198 1105 32-33 -0.4413173378230226 1105 34-35 -0.2479613157315157 1105 36-37 -0.4155525518892702 1105 38-39 -0.38474861964134277 1105 40-41 -0.29318856551128647 1105 42-43 -0.22266132954039364 1105 44-45 -0.19847767681597617 1105 46-47 -0.3515694752827869 1105 48-49 -0.26509482338361323 1105 50-51 -0.42272060763406927 1105 52-53 -0.34764924891441495 1105 54-55 -0.14544588097226097 1105 56-57 -0.24845805910698004 1105 58-59 -0.08176309646850655 1105 60-61 -0.020053432137054017 1105 62-63 -0.06901441872788183 1105 64-65 -0.2915546570075378 1105 66-67 -0.26796256184123024 1105 68-69 -0.14320395348369885 1105 70-71 -0.14490118288589215 1105 72-73 -0.31448577051225257 1105 74-75 -0.01256223836421455 1105 76-77 -0.19410542637761097 1105 78-79 -0.40362163095398174 1105 80-81 -0.17801831884014874 1105 82-83 -0.3938476908429216 1105 84-85 -0.3015697662270753 1105 86-87 -0.12976411436789803 1105 88-89 0.16969931801253324 1105 90-91 0.10271757657896075 1105 92-93 0.3114908238255829 1105 94-95 0.3928480810124668 1105 96-97 0.5284566242911914 1105 98-99 0.40036468416079174 1105 100-101 0.5641502947463515 1106 1 -0.33640797272402523 1106 2 -0.23899402596475738 1106 3 -0.30998536236397456 1106 4 -0.36421640278121004 1106 5 -0.23733166583877363 1106 6 -0.22058912720643775 1106 7 -0.27431440182779454 1106 8 -0.21117973364132325 1106 9 -0.7669360010899382 1106 10-11 -0.6605776266555878 1106 12-13 -0.6173194356460883 1106 14-15 -0.43112087687397604 1106 16-17 -0.40163609990747773 1106 18-19 -0.6123041430924872 1106 20-21 -0.525135661355911 1106 22-23 -0.4178023693831676 1106 24-25 -0.6384058560082764 1106 26-27 -0.6042403845757391 1106 28-29 -0.5880322781900347 1106 30-31 -0.5891441228209615 1106 32-33 -0.7325365147282525 1106 34-35 -0.7376661872840558 1106 36-37 -0.8531266561937727 1106 38-39 -0.6116731836073228 1106 40-41 -0.5636005424852542 1106 42-43 -0.5147353908858676 1106 44-45 -0.47151230178375414 1106 46-47 -0.466375300525506 1106 48-49 -0.768035368103952 1106 50-51 -0.7330608430494934 1106 52-53 -0.6619180864434142 1106 54-55 -0.7671659600168539 1106 56-57 -0.8733807328762069 1106 58-59 -1.048828566010556 1106 60-61 -0.867658466933861 1106 62-63 -1.1047735045599936 1106 64-65 -1.3079134507183383 1106 66-67 -1.2908425343474548 1106 68-69 -1.150193385565494 1106 70-71 -1.2276359539143336 1106 72-73 -1.3292937437058292 1106 74-75 -1.12649599555985 1106 76-77 -0.8271151351154771 1106 78-79 -1.0004899186075598 1106 80-81 -1.1108819669198802 1106 82-83 -1.2122771091778297 1106 84-85 -1.3257471010496538 1106 86-87 -1.2607289738919114 1106 88-89 -1.564281264511738 1106 90-91 -1.9425676712945688 1106 92-93 -2.2096965634069967 1106 94-95 -2.381559084551583 1106 96-97 -2.6177286149905363 1106 98-99 -2.492362354244573 1106 100-101 -2.700292526801892 1107 1 0.09421059787028696 1107 2 0.14194124199265445 1107 3 0.057540804474896845 1107 4 0.06605402532175475 1107 5 0.08341987272643081 1107 6 0.09698420732480173 1107 7 -0.10239939351729532 1107 8 -0.03463779931671951 1107 9 0.15119436142747134 1107 10-11 -0.19106255545482753 1107 12-13 0.03457176421016328 1107 14-15 0.06732932751935294 1107 16-17 -0.06692517047933677 1107 18-19 0.20631361891281585 1107 20-21 0.16362495137487798 1107 22-23 0.248738891117263 1107 24-25 0.013855947145216874 1107 26-27 -0.08687256641842822 1107 28-29 0.2787976036661277 1107 30-31 0.279364704275757 1107 32-33 0.14379428802094907 1107 34-35 -0.03602991757470164 1107 36-37 -0.001980032052891545 1107 38-39 0.014112944598963395 1107 40-41 0.05175254138339369 1107 42-43 0.11119111735725085 1107 44-45 -0.18140853286052305 1107 46-47 -0.7779235238757636 1107 48-49 -0.7410767858300176 1107 50-51 -0.6128393795033773 1107 52-53 -0.6030876206062956 1107 54-55 -0.9156543477802046 1107 56-57 -0.7448431559479403 1107 58-59 -0.406646049501866 1107 60-61 -0.6270639294018494 1107 62-63 -0.6899448805089676 1107 64-65 -0.6641755234015037 1107 66-67 -0.417521479588018 1107 68-69 -0.3576413887998484 1107 70-71 -0.5716568873854264 1107 72-73 -0.3119427711529319 1107 74-75 -0.5168030347764194 1107 76-77 -0.5250846782626866 1107 78-79 -0.41749724083028283 1107 80-81 -0.5673978467096461 1107 82-83 -0.34727778300200995 1107 84-85 -0.22878380200648607 1107 86-87 -0.19636753460346767 1107 88-89 -0.0820477619736657 1107 90-91 -0.1428001406355648 1107 92-93 -0.34269822550663065 1107 94-95 -0.2928894380846643 1107 96-97 -0.4343249044654023 1107 98-99 -0.04224780413451157 1107 100-101 0.4435352437385127 1108 1 0.003722846821951009 1108 2 0.06795095287325381 1108 3 -0.024394750590211345 1108 4 -0.03408943086893146 1108 5 -0.04966333186416705 1108 6 -0.05771398292017693 1108 7 0.13093761817989957 1108 8 0.12094319825555999 1108 9 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-0.2447799532578756 2316 66-67 0.09752883655768585 2316 68-69 0.16968263191183297 2316 70-71 0.06564703231229885 2316 72-73 -0.05034970218610013 2316 74-75 -0.06856632625384051 2316 76-77 0.35082972703990833 2316 78-79 0.5039847636733619 2316 80-81 -0.020179547153649224 2316 82-83 -0.015258012764231665 2316 84-85 -0.05667841162698295 2316 86-87 -0.008438956813819232 2316 88-89 0.13371372295693362 2316 90-91 -0.1705408121112093 2316 92-93 -0.23950559927099135 2316 94-95 -0.2545130402372564 2316 96-97 0.1762751998563168 2316 98-99 0.004992759202764319 2316 100-101 -0.027299202783883203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 15.0 6 24.0 7 73.0 8 132.0 9 273.0 10 540.0 11 885.0 12 1365.0 13 2068.0 14 2613.0 15 3943.0 16 5808.0 17 8590.0 18 7907.0 19 4524.0 20 3447.0 21 3416.0 22 3478.0 23 3697.0 24 4120.0 25 4695.0 26 5315.0 27 6446.0 28 7664.0 29 9462.0 30 11217.0 31 14231.0 32 17717.0 33 23378.0 34 32186.0 35 49466.0 36 69092.0 37 115755.0 38 118583.0 39 13628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38131410406414 21.768111141911863 16.34511882089923 19.505455933124768 2 14.90352867509595 23.412582027166355 41.27709572999905 20.406793567738653 3 17.891863588844846 28.524311709529815 32.41141425900702 21.172410442618318 4 11.431532619237405 19.937886969398715 39.92795464237325 28.702625768990625 5 11.118688963547731 40.49428391993709 35.526431809225265 12.860595307289916 6 27.014490041582622 40.64678881749529 18.47016973123339 13.868551409688697 7 24.860686518125597 36.456858253817735 22.454616282898584 16.22783894515808 8 24.96144925606511 36.479529869854126 20.794730108805766 17.764290765275 9 26.310590343690905 17.217293045031948 22.506976919935006 33.965139691342145 10-11 22.924872382193065 29.575033152740115 28.335651476084145 19.164442988982675 12-13 24.850302903246412 27.80348583917201 29.002682176080675 18.343529081500904 14-15 21.810724394119056 28.881479628833258 29.001405280327337 20.306390696720353 16-17 18.50435708874858 32.021498449873235 30.018119299483054 19.456025161895134 18-19 18.18164642715124 29.611739578703794 34.44106369464572 17.765550299499246 20-21 19.808783851259136 28.34288634361868 34.77094817263254 17.07738163248965 22-23 19.096561521319217 28.894682814084277 34.44443544766619 17.56432021693032 24-25 18.669266105394225 29.007433051257646 34.900253886112914 17.423046957235215 26-27 18.68873756307866 29.242588229155142 34.67619245548169 17.392481752284507 28-29 18.270485999587947 29.256511228489714 35.00845251879662 17.464550253125722 30-31 18.622393438906293 29.48299718764001 34.67173839114295 17.22287098231074 32-33 18.29851320981501 29.67561595721902 34.46949318142151 17.55637765154446 34-35 18.653971761242694 29.507288204821858 34.63413229787625 17.204607736059206 36-37 18.633886270847615 29.538433025825945 34.338355294291226 17.48932540903521 38-39 18.433553272000015 29.875252131761677 34.34983743011834 17.34135716611997 40-41 18.548744062185115 29.8947387361451 34.20730171296963 17.34921548870016 42-43 18.484428249900333 29.9453479132226 33.869805104521106 17.70041873235596 44-45 18.801067365288315 30.25725086862878 33.227412502856446 17.71426926322646 46-47 18.812853006958026 30.413433112435023 32.884999127322715 17.888714753284233 48-49 18.823649014594405 30.588418402874616 32.57956207794358 18.008370504587404 50-51 19.12134892516747 30.861827296265844 31.921815312697365 18.095008465869324 52-53 19.13630061870176 31.13589396871494 31.645286053974687 18.08251935860861 54-55 19.18153666774027 31.001185761505397 31.496902445475662 18.32037512527867 56-57 19.392688583761725 31.159887073760125 31.019898841408448 18.427525501069706 58-59 19.376044738655647 30.91859630308712 31.434105667724076 18.27125329053316 60-61 19.477707143898186 31.152149934954053 31.306712777614838 18.063430143532923 62-63 19.37991330805868 31.008293133199345 31.71588146703349 17.895912091708485 64-65 19.38576114552838 30.92066553788409 31.513096456930228 18.1804768596573 66-67 19.321704833552552 30.98670111792659 31.266227748978427 18.42536629954243 68-69 19.430024776837527 31.137755258105553 31.226732354375354 18.20548761068157 70-71 19.585037453149827 30.99803692594478 31.158447606075274 18.258478014830114 72-73 19.311088759376783 30.69592864558686 31.187776760154094 18.805205834882262 74-75 19.317476397228305 31.19056572879349 30.78292647378999 18.709031400188213 76-77 19.164982789364494 30.836726578511264 31.29546693632695 18.702823695797292 78-79 18.880777888336894 30.686302205444427 31.2941174353724 19.13880247084628 80-81 19.072496990612873 31.077387582072152 30.875142372350705 18.974973054964277 82-83 19.22509786139893 30.91214650915277 30.86635340222992 18.99640222721838 84-85 19.01089953801793 30.829453493655055 30.749922403206437 19.409724565120577 86-87 18.9298097563521 30.73344477212327 30.699887181720197 19.63685828980443 88-89 19.160754353040247 30.51932395400181 30.84590318500219 19.47401850795576 90-91 18.871442158103914 30.253926864096353 31.273197448584334 19.601433529215402 92-93 18.841392042566323 30.625224361194757 30.99814753002504 19.535236066213884 94-95 18.69985479369729 30.52859052722303 30.95971109883565 19.811843580244027 96-97 18.694816656800313 30.32436604943492 30.954403061747765 20.026414232017 98-99 18.732973704382122 30.554395978105582 30.68430863793234 20.02832167957996 100-101 18.610322031473405 30.710189670050237 30.80817851425877 19.871309784217587 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2075.0 1 1372.0 2 811.0 3 921.5 4 741.0 5 755.0 6 1447.0 7 2964.5 8 4227.0 9 4397.5 10 3463.0 11 2392.5 12 2172.5 13 2134.0 14 2029.5 15 1952.5 16 1867.0 17 1790.0 18 1802.5 19 1948.5 20 2152.0 21 2435.5 22 2811.0 23 3346.0 24 3935.5 25 4632.0 26 5601.0 27 6653.0 28 7634.0 29 9021.5 30 10907.0 31 12442.0 32 14282.0 33 16805.5 34 19043.5 35 20771.0 36 22823.5 37 24012.5 38 23901.0 39 23902.5 40 22967.5 41 21442.0 42 20181.0 43 19798.0 44 19685.0 45 18980.5 46 18089.5 47 17451.0 48 17954.0 49 16739.0 50 14448.0 51 12477.0 52 10383.5 53 8990.5 54 7611.0 55 6324.0 56 5253.0 57 4366.5 58 3534.5 59 2929.5 60 2455.5 61 2020.0 62 1460.5 63 890.0 64 554.5 65 366.5 66 284.0 67 212.0 68 152.5 69 116.0 70 84.0 71 66.5 72 47.0 73 34.5 74 29.0 75 15.5 76 9.5 77 5.0 78 4.0 79 3.5 80 1.5 81 2.0 82 2.0 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039405427872772646 2 0.0 3 0.0 4 0.0 5 0.008636806109100855 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.009986307063647863 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.4983365151566955E-4 22-23 0.0011695674939407408 24-25 0.0 26-27 0.0011695674939407408 28-29 0.0017093678757595441 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.598669212125356E-4 38-39 0.0 40-41 1.799334606062678E-4 42-43 0.027979653124274643 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.99667303031339E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.99667303031339E-5 84-85 6.297671121219372E-4 86-87 0.0 88-89 0.0 90-91 0.012505375512135613 92-93 0.0029689021000034186 94-95 0.0 96-97 0.0 98-99 5.398003818188035E-4 100-101 0.015384310881835898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 555761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.69199811629244 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15722372244856 51.50600514847561 2 11.07811781015218 13.890186817722523 3 3.1894004395935394 5.998496590533013 4 1.267354951585203 3.178120569498137 5 0.6280686975287468 1.9687440801187222 6 0.34139609330333665 1.2841681942969425 7 0.22164837786312833 0.972690578123216 8 0.17123420053170377 0.8588011341742731 9 0.11271751599668507 0.635983767048361 >10 0.7412575683247268 8.999862457618837 >50 0.060672166021346466 2.618065786447423 >100 0.027760373103113488 2.921640459583488 >500 0.0011447576537364928 0.5497363281849122 >1k 0.0011447576537364928 1.1513244183950984 >5k 8.585682403023697E-4 3.466173669779477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7563 1.3608367625652034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6667 1.1996163818619874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5089 0.9156813810252968 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2294 0.41276735863077835 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.2697202574487954 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.24668877449119317 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.22545662613965353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 832 0.14970463922441482 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.14898490538198975 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.13153135970318178 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 673 0.12109521898801823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.598669212125356E-4 0.0 14-15 0.0 0.0 0.0 3.598669212125356E-4 0.0 16-17 0.0 0.0 0.0 3.598669212125356E-4 0.0 18-19 0.0 0.0 0.0 3.598669212125356E-4 0.0 20-21 0.0 0.0 0.0 4.498336515156695E-4 0.0 22-23 0.0 0.0 0.0 8.097005727282051E-4 0.0 24-25 0.0 0.0 0.0 0.0012595342242438744 0.0 26-27 0.0 0.0 0.0 0.0020692347969720796 0.0 28-29 0.0 0.0 0.0 0.003868569403034758 0.0 30-31 0.0 0.0 0.0 0.007107371693947578 0.0 32-33 0.0 0.0 0.0 0.01241540878183248 0.0 34-35 0.0 0.0 0.0 0.018083312790929916 0.0 36-37 0.0 0.0 0.0 0.02330138314851168 0.0 38-39 0.0 0.0 0.0 0.03148835560609686 0.0 40-41 0.0 0.0 0.0 0.03805592691822564 0.0 42-43 0.0 0.0 0.0 0.05478973875460855 0.0 44-45 0.0 0.0 0.0 0.07413258576978234 0.0 46-47 0.0 0.0 0.0 0.09266573221222792 0.0 48-49 0.0 0.0 0.0 0.11848618380922735 0.0 50-51 0.0 0.0 0.0 0.15042437306683987 0.0 52-53 0.0 0.0 0.0 0.21403085139115557 0.0 54-55 0.0 0.0 0.0 0.26720118900030765 0.0 56-57 0.0 0.0 0.0 0.3558184183488946 0.0 58-59 0.0 0.0 0.0 0.46584772950962733 0.0 60-61 0.0 0.0 0.0 0.5726382383794473 0.0 62-63 0.0 0.0 0.0 0.7285505819947784 0.0 64-65 0.0 0.0 0.0 0.811679840794874 0.0 66-67 0.0 0.0 0.0 0.9972812054102393 0.0 68-69 0.0 0.0 0.0 1.2239073990438336 0.0 70-71 0.0 0.0 0.0 1.3966435212258506 0.0 72-73 0.0 0.0 0.0 1.6797688214898132 0.0 74-75 0.0 0.0 0.0 1.959205485811347 0.0 76-77 0.0 0.0 0.0 2.1591115605449103 0.0 78-79 0.0 0.0 0.0 2.3900561572330554 0.0 80-81 0.0 0.0 0.0 2.765487322788033 0.0 82-83 0.0 0.0 0.0 3.071284239088385 0.0 84-85 0.0 0.0 0.0 3.5107717166191943 0.0 86-87 0.0 0.0 0.0 4.042205192519806 0.0 88-89 1.799334606062678E-4 0.0 0.0 4.740796853323641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4115 0.0 32.224667 1 GTGTAGC 210 0.0 31.685663 1 AGGGGTC 150 9.531504E-10 31.665705 5 GGGGTCC 140 1.704575E-8 30.532042 6 GTATCAA 5800 0.0 27.943478 1 TAGGGCG 70 5.811926E-7 27.139593 30-31 GTACATG 3605 0.0 25.577055 1 TACATGG 3720 0.0 24.387701 2 TAACGCG 80 1.86141E-6 23.747143 76-77 CATGGGG 765 0.0 22.97316 4 GGTATAC 125 2.2447317E-4 22.813677 1 ACATGGG 3730 0.0 22.794216 3 ATCAACG 7245 0.0 22.225 3 TATCAAC 7280 0.0 22.183393 2 TCAACGC 7295 0.0 22.072668 4 AGTACTT 4815 0.0 21.90359 12-13 GAGTACT 4740 0.0 21.899374 12-13 CAACGCA 7385 0.0 21.80367 5 GTATAGT 350 0.0 21.727312 1 GTATAGG 330 1.8189894E-12 21.60386 1 >>END_MODULE