FastQCFastQC Report
Thu 26 May 2016
SRR937736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369067
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57111.5474155099209628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43881.188944012875711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.8835794042816074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12570.34058856521986525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.24494197530529685No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.2313942996800039No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.21513708892965233No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5790.15688208374089258No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.1441472686531172No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.12490956926520116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.12274194116515429No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG250.005015278257.0094539
GGTATCA20550.044.6545031
GTATCAA33100.033.4693831
ATAGCCC1805.456968E-1231.6848343
GTGTAGC1551.3369572E-930.6752471
GTATAGG804.4620482E-429.7166481
TATAGGG1153.8380094E-628.9296282
CATGGGG3350.028.3783344
TATCAAC40850.027.2249092
ATCAACG40700.027.2084733
TCAACGC40950.027.0460434
CAACGCA41600.026.6234475
GTACATG21450.026.5995161
GGAGTGC908.9272985E-426.3932676
CGACCCG1457.814597E-726.2254945
AACGCAG42400.026.1069226
ACATGGG20550.025.6716523
CCTTTAG1309.921103E-625.6020341
TACATGG21250.025.2733142
TAGCCCT3250.024.8637874
Produced by FastQC (version 0.11.5)