FastQCFastQC Report
Thu 26 May 2016
SRR937736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369067
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49811.3496194457916855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42721.1575134054250311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33840.9169066863198281No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17990.48744536899804103No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.26336681415569535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.2492772315053906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.2468386498928379No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6030.1633849680410332No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.15552731617836327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.13818629137798827No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.12111622009011913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.037.100251
GTAGGAC1201.4963553E-731.6781433
CTTAATC1401.6932972E-830.5467823
GTGCTAC650.005824808529.2453231
GTATCAA38150.028.7757911
TAGGACG856.3853274E-427.9513034
TAGTCGA350.00839146827.14165920-21
CATGGGT3250.026.306536
CTTAGAC1109.436262E-525.918483
TAGGACT2551.8189894E-1124.2244634
ATCAACG46800.023.0478783
GAGTACT31250.023.03010212-13
TCAACGC47000.022.9498024
TATCAAC47700.022.8122462
GTATAGC2303.5852281E-922.7287041
CAACGCA47550.022.684355
AACGCAG48500.022.2309746
TACATGG25550.021.9453282
GTACTTT32550.021.88843214-15
GTGTAGG1754.0195373E-621.72511
Produced by FastQC (version 0.11.5)