##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 369067 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.311325585869234 33.0 31.0 34.0 31.0 34.0 2 32.51792763915495 34.0 31.0 34.0 31.0 34.0 3 32.59028848420476 34.0 31.0 34.0 31.0 34.0 4 36.20395212793341 37.0 35.0 37.0 35.0 37.0 5 36.0800152817781 37.0 35.0 37.0 35.0 37.0 6 36.18631034473415 37.0 36.0 37.0 35.0 37.0 7 36.15566008339949 37.0 36.0 37.0 35.0 37.0 8 36.16146661717249 37.0 36.0 37.0 35.0 37.0 9 37.83014737161545 39.0 38.0 39.0 35.0 39.0 10-11 37.799467305394415 39.0 37.5 39.0 35.0 39.0 12-13 37.725990131873075 39.0 37.0 39.0 35.0 39.0 14-15 38.903617771298975 40.0 38.0 41.0 35.5 41.0 16-17 38.883648497427295 40.0 38.0 41.0 35.5 41.0 18-19 38.957621515876525 40.0 38.0 41.0 35.5 41.0 20-21 38.94032113410302 40.0 38.0 41.0 35.0 41.0 22-23 38.789462346945136 40.0 38.0 41.0 35.0 41.0 24-25 38.73664944305506 40.0 38.0 41.0 35.0 41.0 26-27 38.62918250615742 40.0 38.0 41.0 35.0 41.0 28-29 38.50987625553084 40.0 38.0 41.0 34.0 41.0 30-31 38.45957509070169 40.0 38.0 41.0 35.0 41.0 32-33 38.2670111388989 40.0 37.0 41.0 34.0 41.0 34-35 38.10018777078417 40.0 37.0 41.0 34.0 41.0 36-37 37.92354369260866 40.0 37.0 41.0 34.0 41.0 38-39 37.76603435148631 40.0 36.5 41.0 33.0 41.0 40-41 37.55878878360839 40.0 36.0 41.0 33.0 41.0 42-43 37.29814776178851 40.0 36.0 41.0 33.0 41.0 44-45 36.897239525614594 40.0 36.0 41.0 32.5 41.0 46-47 36.3922024998171 40.0 35.0 41.0 30.5 41.0 48-49 35.932513608640164 40.0 35.0 41.0 29.5 41.0 50-51 35.644629565905376 39.0 35.0 41.0 28.0 41.0 52-53 35.42683171348291 39.0 35.0 41.0 27.0 41.0 54-55 35.22168739009448 39.0 35.0 40.5 26.5 41.0 56-57 34.958342252219786 39.0 35.0 40.0 25.5 41.0 58-59 34.740992827860524 38.5 35.0 40.0 25.0 41.0 60-61 34.42792501090588 38.0 34.5 40.0 23.5 41.0 62-63 34.09190201237173 38.0 34.0 40.0 22.5 41.0 64-65 33.72457439977023 38.0 34.0 40.0 21.0 41.0 66-67 33.31637480457478 37.0 33.5 40.0 18.5 41.0 68-69 32.88048240563367 36.5 33.0 39.0 14.5 41.0 70-71 32.48939487952052 36.0 33.0 39.0 9.5 40.0 72-73 32.02976559811633 35.5 32.0 39.0 6.5 40.0 74-75 31.519869021072054 35.0 31.5 38.0 2.0 40.0 76-77 30.237931053169206 34.0 30.0 36.0 2.0 39.0 78-79 30.719964125754945 35.0 31.0 37.0 2.0 39.0 80-81 30.4756385696906 35.0 31.0 36.0 2.0 39.0 82-83 30.102547775878094 34.5 31.0 36.0 2.0 37.5 84-85 29.66945703625629 34.0 30.5 35.5 2.0 37.0 86-87 29.219021207531426 34.0 30.0 35.0 2.0 37.0 88-89 28.782772504721365 34.0 29.0 35.0 2.0 36.0 90-91 28.30354922006031 34.0 29.0 35.0 2.0 36.0 92-93 27.88416059956593 34.0 28.5 35.0 2.0 35.5 94-95 27.45038434755749 34.0 27.0 35.0 2.0 35.0 96-97 26.998043715639707 33.5 26.5 35.0 2.0 35.0 98-99 26.322635185481225 33.0 25.0 35.0 2.0 35.0 100-101 24.097191024935853 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1070442799703244 1101 2 0.09479327634306145 1101 3 0.07986276423018523 1101 4 0.11869573606939099 1101 5 -0.015231232711528264 1101 6 0.03151997465368339 1101 7 -0.06986877746505371 1101 8 -0.058785016672992185 1101 9 0.06410341070440495 1101 10-11 0.026602502000933725 1101 12-13 0.035441192979213554 1101 14-15 -0.08457739564324385 1101 16-17 -0.09247937594445688 1101 18-19 -0.026396658638780934 1101 20-21 0.1670912065829313 1101 22-23 0.2207962995870716 1101 24-25 -0.009393526712479172 1101 26-27 0.07682964795116476 1101 28-29 -0.0690921171980321 1101 30-31 0.09650920088032677 1101 32-33 0.23107373409316523 1101 34-35 0.06255692485423481 1101 36-37 -0.0036561773974739253 1101 38-39 0.25827254153636403 1101 40-41 -0.1838795138218572 1101 42-43 0.12587968499286006 1101 44-45 0.2475597470607056 1101 46-47 0.05493036511052196 1101 48-49 -0.0076840912101303616 1101 50-51 -0.12475718423549864 1101 52-53 0.05699536649153458 1101 54-55 0.08417530021922204 1101 56-57 0.02499507987488414 1101 58-59 -0.15810050620127925 1101 60-61 -0.10022770237858225 1101 62-63 0.0052384565528527105 1101 64-65 0.03856623390218772 1101 66-67 0.10584609646998899 1101 68-69 0.14126719977841873 1101 70-71 0.01963013327405605 1101 72-73 0.0606361593097553 1101 74-75 -0.12521461000368816 1101 76-77 -0.26105429962823834 1101 78-79 0.08907349048681112 1101 80-81 0.08851065575373696 1101 82-83 0.15249028231098904 1101 84-85 0.374319574915571 1101 86-87 0.1876339843938588 1101 88-89 0.2807600572119604 1101 90-91 0.2812273487817265 1101 92-93 0.5634583312041919 1101 94-95 0.5541718552038581 1101 96-97 0.5339732196344791 1101 98-99 0.649959127270499 1101 100-101 1.255345970102507 1102 1 0.007190692854656788 1102 2 0.17338808567991038 1102 3 0.14302173878045465 1102 4 0.0628741919263689 1102 5 -0.17962772253257242 1102 6 -0.06403075228162436 1102 7 -0.017400741169581124 1102 8 0.008365340966129509 1102 9 0.19020127419537403 1102 10-11 0.10263928240958364 1102 12-13 0.03872289323380329 1102 14-15 0.05151384662811864 1102 16-17 0.17110298363439114 1102 18-19 0.0854814363150993 1102 20-21 0.1916494033730416 1102 22-23 0.09009155935337532 1102 24-25 -0.03330545516600836 1102 26-27 -0.08017718775865745 1102 28-29 -0.24132962314313744 1102 30-31 -0.24316496050956715 1102 32-33 0.037035629440588025 1102 34-35 0.1483231678250192 1102 36-37 0.1074283037693533 1102 38-39 0.09789261409000716 1102 40-41 0.16381098612685463 1102 42-43 0.4494661478627435 1102 44-45 0.5111980606080948 1102 46-47 0.6844125111317325 1102 48-49 0.28655451516302577 1102 50-51 0.27258966508978943 1102 52-53 0.38451539334943163 1102 54-55 0.4107664911994391 1102 56-57 0.6327801420304198 1102 58-59 0.77492725629277 1102 60-61 0.6989339206595275 1102 62-63 0.5397713808005022 1102 64-65 0.6389588442480729 1102 66-67 0.6817569804494354 1102 68-69 0.721462292615378 1102 70-71 0.7729980958968099 1102 72-73 0.9501177457912711 1102 74-75 1.1098815841938858 1102 76-77 1.083276164509929 1102 78-79 1.3443084505263485 1102 80-81 1.4304910532134265 1102 82-83 1.3174668282725115 1102 84-85 1.6109246611313104 1102 86-87 1.4236524865061249 1102 88-89 1.396123151839447 1102 90-91 1.3936080968862825 1102 92-93 1.4471915799848638 1102 94-95 1.1522088034744158 1102 96-97 1.2881012236631655 1102 98-99 1.339947097167265 1102 100-101 1.8004644526308802 1103 1 0.09565399521320472 1103 2 0.1112791025014559 1103 3 0.1751737138829057 1103 4 -0.012642483691841733 1103 5 -0.1366088212913752 1103 6 -0.013855522791075714 1103 7 -0.05934055247598735 1103 8 -0.025770688130059227 1103 9 0.13411802731186384 1103 10-11 0.14128419069920284 1103 12-13 0.10768777621385794 1103 14-15 0.2599829858822176 1103 16-17 0.12328948961475561 1103 18-19 0.16322128244669187 1103 20-21 0.1417746023302371 1103 22-23 0.12201790656036593 1103 24-25 -0.06878176301075456 1103 26-27 0.12559790537416404 1103 28-29 0.1927073258772225 1103 30-31 0.20324350936542857 1103 32-33 0.4581688700702884 1103 34-35 0.3950975472869942 1103 36-37 0.4286145179049399 1103 38-39 0.482031463534895 1103 40-41 0.6852677084892704 1103 42-43 0.8898268091486656 1103 44-45 0.7996866234810227 1103 46-47 1.1051656178071312 1103 48-49 1.2766035892302838 1103 50-51 1.1646953864736744 1103 52-53 1.2085354448024077 1103 54-55 1.3322393999108826 1103 56-57 1.3242205063704233 1103 58-59 1.2951050118347567 1103 60-61 1.0778612562148098 1103 62-63 1.3296737967357188 1103 64-65 1.447519706146636 1103 66-67 1.4823571016018064 1103 68-69 1.4467713660449917 1103 70-71 1.707406595201526 1103 72-73 1.4963604282126504 1103 74-75 1.45998740858181 1103 76-77 1.3664526619311488 1103 78-79 1.2178906121887358 1103 80-81 1.3008739584668767 1103 82-83 1.2173677481966827 1103 84-85 1.392762106042543 1103 86-87 1.297525266707769 1103 88-89 1.2210346787271789 1103 90-91 1.3618779478492513 1103 92-93 1.5442262898936043 1103 94-95 1.248432969693507 1103 96-97 1.3493089214983591 1103 98-99 1.6405692906137546 1103 100-101 2.2123432708312016 1104 1 -0.07529658245267257 1104 2 -0.00729804057188943 1104 3 0.04747154802280562 1104 4 -0.07423380786919154 1104 5 -0.256087116256964 1104 6 -0.16882269265821037 1104 7 -0.06203712913065118 1104 8 0.006065426047946687 1104 9 -0.05468705215804448 1104 10-11 -0.09639806039198362 1104 12-13 -0.027087744098174937 1104 14-15 -0.05069256848097581 1104 16-17 -0.17264260067928916 1104 18-19 0.017086776927989433 1104 20-21 -0.012088484493332885 1104 22-23 -0.13244399913511984 1104 24-25 -0.16214729016741103 1104 26-27 -0.26488290387398905 1104 28-29 -0.13095926168069383 1104 30-31 -0.09011238716032466 1104 32-33 -0.18323458801410908 1104 34-35 -0.12497887864829949 1104 36-37 -0.2284524734515685 1104 38-39 -0.3362591682688887 1104 40-41 -0.17128060460253636 1104 42-43 -0.004392748599002516 1104 44-45 -0.07853920793478153 1104 46-47 -0.02901658685065911 1104 48-49 0.10584734393811601 1104 50-51 -0.22141552399929054 1104 52-53 0.16967928859357073 1104 54-55 0.16768803253327036 1104 56-57 0.2083691757955748 1104 58-59 0.1448723289129603 1104 60-61 0.18635203316596005 1104 62-63 -0.021422891359094365 1104 64-65 0.01948803512898678 1104 66-67 0.39246376083912793 1104 68-69 0.6160916154783322 1104 70-71 0.6405786154671276 1104 72-73 0.6121670451439876 1104 74-75 0.5571759522584401 1104 76-77 0.02778808648719533 1104 78-79 0.09390483800639515 1104 80-81 0.337043856554164 1104 82-83 0.5744079113670857 1104 84-85 0.6519533394168064 1104 86-87 0.7989354662338037 1104 88-89 0.6036716830506457 1104 90-91 0.7993717137354892 1104 92-93 1.026371788089584 1104 94-95 1.193094676585254 1104 96-97 1.5186068141099476 1104 98-99 1.3439441388453304 1104 100-101 1.9787454390989616 1105 1 0.11653982893174941 1105 2 0.11116478213602932 1105 3 0.11098034347001118 1105 4 0.05159562077709268 1105 5 0.0029017881355812847 1105 6 -0.0697482405837988 1105 7 -0.031863901168016184 1105 8 -0.24424684736342073 1105 9 -0.07943738575744419 1105 10-11 0.11187154885497108 1105 12-13 -0.1489962373256546 1105 14-15 0.3220887670133905 1105 16-17 0.30575721896378383 1105 18-19 0.28208961023796775 1105 20-21 0.1166290923868587 1105 22-23 -0.0848870435114506 1105 24-25 -0.011914836886553815 1105 26-27 0.09490785770754684 1105 28-29 -0.1832749594365879 1105 30-31 -0.21366298154610064 1105 32-33 -0.005400211738354699 1105 34-35 -0.1388133524142816 1105 36-37 -0.1567057530840259 1105 38-39 -0.09355417981539205 1105 40-41 -0.24826742514129307 1105 42-43 -0.08659834331990623 1105 44-45 -0.6462522697984241 1105 46-47 -0.36732293773019364 1105 48-49 0.08693119163399388 1105 50-51 0.10293753688944918 1105 52-53 0.07284711194127169 1105 54-55 0.05693607815869939 1105 56-57 0.01671860517303969 1105 58-59 -0.14723278993372446 1105 60-61 0.3564450754096953 1105 62-63 -0.1014258160661683 1105 64-65 0.02002118485571458 1105 66-67 0.17908505392524177 1105 68-69 0.2762650262351585 1105 70-71 0.3711034175924155 1105 72-73 0.5081876155837328 1105 74-75 0.5089761703928417 1105 76-77 0.6381896853270597 1105 78-79 0.9716246632551488 1105 80-81 0.8710070174313351 1105 82-83 1.2237995113646463 1105 84-85 0.8131503976489434 1105 86-87 1.1039336687575805 1105 88-89 0.8409294045819706 1105 90-91 0.8010595134417997 1105 92-93 1.4106752508208942 1105 94-95 0.9041671301098297 1105 96-97 1.3729533553391775 1105 98-99 1.485627917079416 1105 100-101 1.810315824002597 1106 1 -0.2535291967321811 1106 2 -0.1846989958757348 1106 3 0.03360565855248865 1106 4 -0.035138476199179536 1106 5 0.014739317250800354 1106 6 -0.177073044089326 1106 7 -0.13857183433330533 1106 8 -0.2995950068563076 1106 9 -0.1509092596606365 1106 10-11 -0.13893410508077153 1106 12-13 -0.18276988845356357 1106 14-15 -0.08788538824649095 1106 16-17 -0.32339734959923305 1106 18-19 0.12873883436397904 1106 20-21 -0.14715211008488183 1106 22-23 -0.05326712072660911 1106 24-25 0.14972114579853013 1106 26-27 -0.09174328084366579 1106 28-29 -0.06388927720989557 1106 30-31 -0.3163346682610566 1106 32-33 -0.026926859473483944 1106 34-35 -0.18525943475584228 1106 36-37 -0.09752874320346905 1106 38-39 -0.681357164191553 1106 40-41 -0.2162380280788696 1106 42-43 0.39581553413561466 1106 44-45 0.15194072553249782 1106 46-47 -0.4465535515161889 1106 48-49 -0.6455970195483687 1106 50-51 -0.5725301678210997 1106 52-53 -0.2319825573949572 1106 54-55 -0.2561415730543004 1106 56-57 -0.34216698665024126 1106 58-59 -0.17269996285950384 1106 60-61 0.1002311703013703 1106 62-63 0.01172203073726763 1106 64-65 0.18943717881463584 1106 66-67 0.23669329871641054 1106 68-69 0.06358302289555695 1106 70-71 0.4654260833231447 1106 72-73 0.6640537334485082 1106 74-75 0.3852138994591314 1106 76-77 0.17695679550223176 1106 78-79 0.5409215374242322 1106 80-81 0.3314209587010204 1106 82-83 0.011319586239942225 1106 84-85 0.3428080346101119 1106 86-87 0.39750545438450047 1106 88-89 0.07970076903535173 1106 90-91 0.4194507581372484 1106 92-93 0.4237943812464273 1106 94-95 -0.17007534247666456 1106 96-97 0.5178487532737286 1106 98-99 0.790287415287299 1106 100-101 1.503566411624778 1107 1 -0.15009857717252828 1107 2 0.0383503562058749 1107 3 -0.2938196043566421 1107 4 -0.08638114034506827 1107 5 -0.44124555808891586 1107 6 -0.15477992502201232 1107 7 -0.08323290802663763 1107 8 -0.21786669932155434 1107 9 -0.05519851878860749 1107 10-11 -0.027094331950671346 1107 12-13 0.0070564142759650395 1107 14-15 -0.11080870149238109 1107 16-17 -0.2274108987740746 1107 18-19 -0.281143398098898 1107 20-21 -0.13094430355449305 1107 22-23 -0.16492965174397511 1107 24-25 0.07600812047260064 1107 26-27 -0.2739087855898177 1107 28-29 -0.1726174665832474 1107 30-31 -0.08989802246956913 1107 32-33 -0.04262263323378335 1107 34-35 0.10342857973862607 1107 36-37 -0.20099874989928423 1107 38-39 -0.04655469067940743 1107 40-41 0.1991190163104264 1107 42-43 -0.018202347624203696 1107 44-45 0.32750888497162833 1107 46-47 0.20961644336348684 1107 48-49 -0.10209392765818137 1107 50-51 -0.5968320308326369 1107 52-53 -0.47767951276933474 1107 54-55 -0.4856504154663739 1107 56-57 -0.3262939707772219 1107 58-59 -0.3541761927228322 1107 60-61 -0.5389038881885284 1107 62-63 -0.24021778571867713 1107 64-65 -0.0540540969346992 1107 66-67 0.08148828867269486 1107 68-69 0.10513645154575357 1107 70-71 0.035889669915761147 1107 72-73 -0.18316356538169032 1107 74-75 -0.08006634001465329 1107 76-77 -0.002884868210397684 1107 78-79 -0.05411962213305799 1107 80-81 -0.3870988727220315 1107 82-83 -0.5286977440999685 1107 84-85 -0.3668693847447244 1107 86-87 -0.147612510088301 1107 88-89 -0.6157775877333265 1107 90-91 -0.057094202278676676 1107 92-93 -0.049520834004272984 1107 94-95 -0.01276312064831231 1107 96-97 -0.034634605661242546 1107 98-99 0.43800886946036854 1107 100-101 0.6481762820410992 1108 1 0.09944934326146182 1108 2 0.0779478894742951 1108 3 0.11078001543285154 1108 4 0.12551666648944604 1108 5 -0.24849737799895877 1108 6 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2316 50-51 0.19917970293843013 2316 52-53 0.008778013107281879 2316 54-55 0.15677355060905285 2316 56-57 0.17765781740692432 2316 58-59 0.09034678084578474 2316 60-61 0.38073801900524984 2316 62-63 0.5652326421383904 2316 64-65 0.6078476251329903 2316 66-67 0.34312706483428457 2316 68-69 0.2634967634330394 2316 70-71 0.26417383388456983 2316 72-73 0.33553903215907965 2316 74-75 0.5402504110074915 2316 76-77 0.6972352053536568 2316 78-79 0.2587936057179334 2316 80-81 0.3328561376894079 2316 82-83 0.28024089678887876 2316 84-85 0.4429508088928955 2316 86-87 0.577966198892895 2316 88-89 0.6987239549839828 2316 90-91 1.1499642993856298 2316 92-93 0.5571470485972192 2316 94-95 0.2683458119129014 2316 96-97 0.9698111560459246 2316 98-99 0.6267067552536894 2316 100-101 0.2319085939891785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 11.0 6 18.0 7 46.0 8 76.0 9 186.0 10 316.0 11 537.0 12 868.0 13 1368.0 14 1737.0 15 2553.0 16 3824.0 17 6042.0 18 5538.0 19 3039.0 20 2283.0 21 2186.0 22 2287.0 23 2385.0 24 2675.0 25 2912.0 26 3431.0 27 3837.0 28 4699.0 29 5707.0 30 6718.0 31 8480.0 32 10923.0 33 14541.0 34 20677.0 35 32156.0 36 46313.0 37 78435.0 38 82421.0 39 9836.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63831286763709 21.75191781193245 16.333523081510396 19.276246238920063 2 14.939834772548075 23.607095730585502 41.41334771193306 20.03972178493336 3 18.148195314129957 28.748167676871677 32.27787908428551 20.825757924712857 4 11.41500052835935 19.93432086856858 39.979732677264565 28.670945925807512 5 11.118578660393926 40.68627982352495 35.30666334240279 12.888478173678333 6 27.007833266046543 40.79774133151975 18.345720424746727 13.84870497768698 7 24.765963903573063 36.87785686609749 22.32223417428272 16.033945056046733 8 25.072683279729695 36.43593168720043 20.691364982509953 17.800020050559926 9 26.535019386723818 17.394944549363668 22.41760981068478 33.65242625322774 10-11 22.96981306917172 29.784429385450338 28.100317828470168 19.14543971690777 12-13 25.057039269030252 28.085946391216222 28.83626342658328 18.020750913170243 14-15 21.92311965036159 29.10975513931075 28.963033812288824 20.004091398038838 16-17 18.409529976941858 32.324483088436516 29.906493942834228 19.3594929917874 18-19 18.137899080654734 29.84552940252041 34.28672842600395 17.729843090820907 20-21 19.885007329292513 28.635180062156735 34.65657997057445 16.8232326379763 22-23 19.24926706876277 29.066752288208615 34.3946611175237 17.289319525504922 24-25 18.712506706117605 29.164837725501673 34.95919429045212 17.16346127792861 26-27 18.69850959799383 29.420164389945846 34.77333723512206 17.10798877693826 28-29 18.217276912363605 29.52034769508398 34.95836709035683 17.304008302195584 30-31 18.73128727304256 29.73782538129933 34.46528679074532 17.065600554912795 32-33 18.249938358075905 29.977619239867014 34.458919383201426 17.313523018855655 34-35 18.672761314341297 29.834691262020176 34.600763546998245 16.891783876640286 36-37 18.56559920014523 29.78605510652537 34.40337391313773 17.244971780191673 38-39 18.3423335058404 30.253178962085475 34.360698734918046 17.043788797156072 40-41 18.530646197031974 30.323220445068237 34.119008201762824 17.02712515613696 42-43 18.396649513171337 30.36805493454302 33.700521108554796 17.534774443730843 44-45 18.749983065405466 30.750243180777474 33.07908862076533 17.420685133051723 46-47 18.788322987425047 30.987462980976353 32.657484955306224 17.566729076292383 48-49 18.825579095394605 31.102618223791346 32.33559218244926 17.736210498364795 50-51 19.07011464043114 31.42207241503575 31.610927013252336 17.89688593128077 52-53 19.154110229307957 31.66186627360344 31.293369496595467 17.890654000493136 54-55 19.12132485429475 31.52232521466292 31.2000260115372 18.15632391950513 56-57 19.352583677218497 31.606591757052243 30.696187955032556 18.344636610696703 58-59 19.43305687043274 31.46488307001168 31.118469004272935 17.983591055282645 60-61 19.380491889006603 31.63151948020278 30.998165644720338 17.98982298607028 62-63 19.266691413754142 31.36232716552821 31.507287294718843 17.8636941259988 64-65 19.40989034511349 31.353250222859263 31.217637989850083 18.019221442177166 66-67 19.270620239685478 31.385222737334956 31.081077419547125 18.263079603432438 68-69 19.339577908618217 31.455535173830224 31.124700935060574 18.080185982490985 70-71 19.439695231489136 31.20910295420615 31.158028217098792 18.193173597205927 72-73 19.35895108476239 30.762707042352744 31.075522872540756 18.802819000344112 74-75 19.298257497961075 31.33333513969008 30.729650713827027 18.638756648521813 76-77 19.14313661205147 30.917692451506095 31.320058417577297 18.619112518865137 78-79 18.841836306144955 30.74075980783977 31.094354141659913 19.323049744355362 80-81 19.004950320673483 31.172388753261604 30.84981859662338 18.972842329441537 82-83 19.142756115767753 31.058088447474912 30.7300987762368 19.06905666052053 84-85 18.949972430442752 30.923695323200562 30.472286159811308 19.654046086545378 86-87 18.91052302156519 30.770835647727917 30.573716967379905 19.744924363326984 88-89 19.027845892480226 30.48782470391555 30.730192620852044 19.754136782752184 90-91 18.795684472800335 30.16411522343814 31.17674395288309 19.86345635087843 92-93 18.777400842144054 30.368939299513897 31.003121460583433 19.85053839775862 94-95 18.453559922724057 30.540660638854188 30.91931817258113 20.08646126584062 96-97 18.427683862279746 30.462761504008757 30.722064015476864 20.38749061823463 98-99 18.53552336025708 30.87501727328642 30.293957465717607 20.29550190073889 100-101 18.466789425405096 31.13410917939984 30.082665680260913 20.316435714934155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1352.0 1 876.5 2 506.5 3 621.5 4 522.5 5 531.5 6 923.5 7 1859.5 8 2688.5 9 2764.0 10 2212.5 11 1642.5 12 1569.0 13 1562.0 14 1454.0 15 1367.5 16 1322.5 17 1306.0 18 1321.5 19 1403.5 20 1523.0 21 1702.5 22 2000.0 23 2387.5 24 2742.0 25 3192.5 26 3775.5 27 4475.5 28 5174.5 29 6034.0 30 7246.5 31 8312.5 32 9683.0 33 11333.0 34 12685.5 35 13739.5 36 15161.0 37 15966.0 38 15820.5 39 15788.5 40 15159.5 41 14218.0 42 13324.5 43 12959.5 44 12931.5 45 12539.0 46 11835.0 47 11478.5 48 11986.0 49 11168.5 50 9619.0 51 8267.0 52 6802.5 53 5775.5 54 4822.0 55 4033.0 56 3410.5 57 2918.0 58 2363.0 59 1932.5 60 1582.0 61 1266.5 62 946.0 63 604.0 64 360.0 65 219.0 66 179.5 67 134.5 68 99.5 69 76.5 70 45.5 71 38.5 72 31.0 73 20.0 74 15.5 75 9.0 76 3.5 77 4.0 78 6.0 79 4.0 80 2.5 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04253970146341992 2 0.0 3 0.0 4 0.0 5 0.01707007128786914 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006231930787634765 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7095351250585936E-4 24-25 2.7095351250585936E-4 26-27 0.0014902443187822265 28-29 0.002167628100046875 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01246386157526953 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3547675625292968E-4 84-85 1.3547675625292968E-4 86-87 0.0 88-89 0.0 90-91 0.007451221593911133 92-93 0.0024385816125527342 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0136831523815459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 369067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.76964278011914 #Duplication Level Percentage of deduplicated Percentage of total 1 84.34072267490227 55.470592021454166 2 9.938757344896434 13.073370405042475 3 2.657685645673596 5.243851066134081 4 1.025687169941533 2.698363150848239 5 0.5252676014901738 1.7273331256989353 6 0.2913454843987033 1.1497013060702093 7 0.18946554137552965 0.8722756682787327 8 0.1376190006262949 0.7240922008758726 9 0.11606085693441931 0.6869952991198144 >10 0.6988651876766011 8.613250378829544 >50 0.052073473698373805 2.315581683399227 >100 0.02231720301324753 2.280245890574856 >500 0.0024796892236941697 1.2204439204531057 >1k 0.0016531261491294466 3.9239038832207203 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4981 1.3496194457916855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4272 1.1575134054250311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3384 0.9169066863198281 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1799 0.48744536899804103 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 0.26336681415569535 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.2492772315053906 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.2468386498928379 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 603 0.1633849680410332 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 574 0.15552731617836327 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.13818629137798827 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 447 0.12111622009011913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.7095351250585936E-4 0.0 22-23 0.0 0.0 0.0 5.419070250117187E-4 0.0 24-25 0.0 0.0 0.0 8.128605375175781E-4 0.0 26-27 0.0 0.0 0.0 0.0016257210750351562 0.0 28-29 0.0 0.0 0.0 0.00433525620009375 0.0 30-31 0.0 0.0 0.0 0.011515524281499023 0.0 32-33 0.0 0.0 0.0 0.018695792362904296 0.0 34-35 0.0 0.0 0.0 0.02221818802548047 0.0 36-37 0.0 0.0 0.0 0.027366304763091796 0.0 38-39 0.0 0.0 0.0 0.036714200944543945 0.0 40-41 0.0 0.0 0.0 0.045520190100984376 0.0 42-43 0.0 0.0 0.0 0.06516431975765918 0.0 44-45 0.0 0.0 0.0 0.08751798453939258 0.0 46-47 0.0 0.0 0.0 0.10689116068356153 0.0 48-49 0.0 0.0 0.0 0.1350703259841709 0.0 50-51 0.0 0.0 0.0 0.16677188694735645 0.0 52-53 0.0 0.0 0.0 0.24426459152403224 0.0 54-55 0.0 0.0 0.0 0.31159653938173826 0.0 56-57 0.0 0.0 0.0 0.43325466649686917 0.0 58-59 0.0 0.0 0.0 0.5639897362809463 0.0 60-61 0.0 0.0 0.0 0.6997374460463819 0.0 62-63 0.0 0.0 0.0 0.8952304053193594 0.0 64-65 0.0 0.0 0.0 1.0125532762343965 0.0 66-67 0.0 0.0 0.0 1.2579016818084523 0.0 68-69 0.0 0.0 0.0 1.5669241628213848 0.0 70-71 0.0 0.0 0.0 1.7922220084700067 0.0 72-73 0.0 0.0 0.0 2.1790081475721212 0.0 74-75 0.0 0.0 0.0 2.5728390779993875 0.0 76-77 0.0 0.0 0.0 2.837289706205106 0.0 78-79 0.0 0.0 0.0 3.1407576402116693 0.0 80-81 0.0 0.0 0.0 3.664510779885495 0.0 82-83 0.0 0.0 0.0 4.085301584807095 0.0 84-85 0.0 0.0 0.0 4.7075463262768 0.0 86-87 0.0 0.0 0.0 5.446572031636532 0.0 88-89 0.0 0.0 0.0 6.472808460252475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2690 0.0 37.10025 1 GTAGGAC 120 1.4963553E-7 31.678143 3 CTTAATC 140 1.6932972E-8 30.546782 3 GTGCTAC 65 0.0058248085 29.245323 1 GTATCAA 3815 0.0 28.775791 1 TAGGACG 85 6.3853274E-4 27.951303 4 TAGTCGA 35 0.008391468 27.141659 20-21 CATGGGT 325 0.0 26.30653 6 CTTAGAC 110 9.436262E-5 25.91848 3 TAGGACT 255 1.8189894E-11 24.224463 4 ATCAACG 4680 0.0 23.047878 3 GAGTACT 3125 0.0 23.030102 12-13 TCAACGC 4700 0.0 22.949802 4 TATCAAC 4770 0.0 22.812246 2 GTATAGC 230 3.5852281E-9 22.728704 1 CAACGCA 4755 0.0 22.68435 5 AACGCAG 4850 0.0 22.230974 6 TACATGG 2555 0.0 21.945328 2 GTACTTT 3255 0.0 21.888432 14-15 GTGTAGG 175 4.0195373E-6 21.7251 1 >>END_MODULE