FastQCFastQC Report
Thu 26 May 2016
SRR937735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444080
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125210.5122991064122288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98260.4020326666884881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64980.2658669110667409No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34340.14050276586691107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63550.052.3937451
GTATCAA109950.038.805581
ATCAACG139250.030.3211213
TATCAAC139900.030.3161892
TCAACGC140800.029.954794
CAACGCA142750.029.4462765
AACGCAG146550.028.7523296
GTACATG114850.027.3758351
TACATGG117850.026.2247432
ACATGGG117100.025.2963943
ACGCAGA168400.024.8257627
CGCAGAG168550.024.5499928
GCAGAGT176900.022.8809369
GAGTACT98000.022.29668412-13
CATGGGA67550.021.7509614
GTACTTT105550.020.63302414-15
TGGTATC30950.020.4323562
GTCTAGC4200.020.3854431
CAGAGTA173650.020.33913810-11
AGTACTT103550.020.23005312-13
Produced by FastQC (version 0.11.5)