##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937735_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444080 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.576382524303625 33.0 31.0 34.0 30.0 34.0 2 31.725095741546923 33.0 31.0 34.0 30.0 34.0 3 31.738048263559293 33.0 31.0 34.0 30.0 34.0 4 35.3443500212759 37.0 35.0 37.0 33.0 37.0 5 35.337047068835716 37.0 35.0 37.0 33.0 37.0 6 35.40040301463127 37.0 35.0 37.0 33.0 37.0 7 35.33083164217211 37.0 35.0 37.0 33.0 37.0 8 35.324365814539625 37.0 35.0 37.0 33.0 37.0 9 36.92673725900953 39.0 37.0 39.0 33.0 39.0 10-11 36.79390629602959 39.0 37.0 39.0 33.0 39.0 12-13 36.674591666393894 39.0 37.0 39.0 32.0 39.0 14-15 37.78503424601486 40.0 37.5 41.0 32.5 41.0 16-17 37.694923038525744 40.0 37.0 41.0 32.0 41.0 18-19 37.63989333409708 40.0 37.0 41.0 32.0 41.0 20-21 37.55440513403816 40.0 37.0 41.0 32.0 41.0 22-23 37.459496415829264 40.0 37.0 41.0 32.0 41.0 24-25 37.49069465811266 40.0 37.0 41.0 32.0 41.0 26-27 37.40428852574384 40.0 37.0 41.0 32.0 41.0 28-29 37.27434863015941 40.0 37.0 41.0 31.5 41.0 30-31 37.0268477300252 39.0 36.5 41.0 31.0 41.0 32-33 36.881363948806914 39.0 36.0 41.0 31.0 41.0 34-35 36.8618318549311 39.0 36.0 41.0 30.5 41.0 36-37 36.7089121469019 39.0 36.0 41.0 30.0 41.0 38-39 36.55636599456646 39.0 35.5 41.0 30.0 41.0 40-41 36.319475221760335 39.0 35.0 41.0 30.0 41.0 42-43 36.04210970999313 39.0 35.0 41.0 29.5 41.0 44-45 35.786123612975025 39.0 35.0 40.0 28.5 41.0 46-47 35.40282151157082 39.0 35.0 40.0 26.5 41.0 48-49 35.055550759385945 39.0 35.0 40.0 25.0 41.0 50-51 34.16917735916991 37.5 33.5 39.5 23.5 40.5 52-53 34.27827669307061 38.0 33.5 39.5 24.0 40.5 54-55 34.55828512159995 38.0 34.0 40.0 24.0 41.0 56-57 34.33261697653104 38.0 34.0 40.0 23.0 41.0 58-59 34.07625057281267 38.0 34.0 40.0 22.5 41.0 60-61 33.7500122745573 38.0 33.0 40.0 21.5 41.0 62-63 33.37472525449249 37.0 33.0 40.0 19.5 41.0 64-65 32.97021435468561 37.0 32.0 39.5 18.0 41.0 66-67 32.5314748289745 36.0 31.5 39.0 15.5 41.0 68-69 32.01278047363425 35.5 31.0 39.0 11.5 40.0 70-71 31.54752974534385 35.0 31.0 38.5 8.0 40.0 72-73 31.07713372721024 35.0 30.5 37.5 7.0 40.0 74-75 30.524576527773235 35.0 30.0 37.0 2.0 39.0 76-77 30.02543472390429 34.0 29.0 36.5 2.0 39.0 78-79 29.446790612418578 34.0 29.0 36.0 2.0 38.5 80-81 28.854248428856664 34.0 28.5 35.5 2.0 37.0 82-83 28.32479296913358 34.0 27.0 35.0 2.0 37.0 84-85 27.78829886910412 33.0 26.0 35.0 2.0 36.0 86-87 27.223742880756767 33.0 26.0 35.0 2.0 36.0 88-89 26.7233916238421 32.5 25.0 35.0 2.0 35.5 90-91 26.14173779090701 32.0 24.5 35.0 2.0 35.0 92-93 25.46943246538575 32.0 21.0 34.0 2.0 35.0 94-95 24.69915121436287 31.5 18.0 34.0 2.0 35.0 96-97 23.833263231972765 31.0 4.5 34.0 2.0 35.0 98-99 22.750624570390492 31.0 2.0 34.0 2.0 35.0 100-101 19.974485491473274 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08917675637667344 1101 2 0.2686166931086156 1101 3 0.2389239226968911 1101 4 0.11613699939385214 1101 5 0.07206405291591977 1101 6 0.24351928425950575 1101 7 0.23640604161774093 1101 8 0.24747991236107936 1101 9 0.3525236932520386 1101 10-11 0.33927493103608697 1101 12-13 0.2595092955571019 1101 14-15 0.43438156984426257 1101 16-17 0.5025107359081318 1101 18-19 0.49313166851934653 1101 20-21 0.43725070867832017 1101 22-23 0.3766652280448355 1101 24-25 0.43849960356470774 1101 26-27 0.46501197590825427 1101 28-29 0.4018119973555301 1101 30-31 0.42457657498656687 1101 32-33 0.37557877103085247 1101 34-35 0.5164393222654056 1101 36-37 0.6262436031553662 1101 38-39 0.5252197025893963 1101 40-41 0.637718384855539 1101 42-43 0.5295669564270113 1101 44-45 0.6061425519131944 1101 46-47 0.6630080074260505 1101 48-49 0.8642828607088617 1101 50-51 0.8671417151503604 1101 52-53 0.7505025340503764 1101 54-55 0.8460097324075022 1101 56-57 0.6915982587110321 1101 58-59 0.6762036450171607 1101 60-61 0.9259946107552608 1101 62-63 1.0492132278265345 1101 64-65 1.033551132331084 1101 66-67 0.8857287448045454 1101 68-69 0.6301436666541953 1101 70-71 0.7538616262709006 1101 72-73 0.8448438745395173 1101 74-75 0.787881830341643 1101 76-77 0.8519650709614766 1101 78-79 0.9675562241868043 1101 80-81 1.0780539709412764 1101 82-83 1.0613162736423547 1101 84-85 0.9823856439670315 1101 86-87 0.9434746534667369 1101 88-89 1.0179485932489598 1101 90-91 1.1361967550512624 1101 92-93 1.245540528237271 1101 94-95 1.2750861049112459 1101 96-97 1.4718488556065985 1101 98-99 1.766366657353828 1101 100-101 1.8739203036512215 1102 1 0.20119910724864454 1102 2 0.2561857087059032 1102 3 0.2778216566957674 1102 4 0.11390866472584094 1102 5 0.1416784096372723 1102 6 0.016342041971370236 1102 7 0.10210878472726392 1102 8 0.08863193606245545 1102 9 0.1718207338505593 1102 10-11 0.08427990575103195 1102 12-13 0.2186079595058814 1102 14-15 0.3422696846541413 1102 16-17 0.370907105157535 1102 18-19 0.4246884535979447 1102 20-21 0.4428400092956508 1102 22-23 0.5048308718088208 1102 24-25 0.4726040968454157 1102 26-27 0.31388509482768256 1102 28-29 0.42182216835278297 1102 30-31 0.41580461739666674 1102 32-33 0.41582964859588856 1102 34-35 0.37103468222269953 1102 36-37 0.5466012128866069 1102 38-39 0.443867023259628 1102 40-41 0.3288292075773782 1102 42-43 0.2210618292526263 1102 44-45 0.2592223910763849 1102 46-47 0.39175521277574177 1102 48-49 0.34793277160712677 1102 50-51 0.20405072376300382 1102 52-53 0.2897315640178917 1102 54-55 0.2611742250762532 1102 56-57 0.29480803130905997 1102 58-59 0.23740160701941448 1102 60-61 0.40149664548225417 1102 62-63 0.4941083684321299 1102 64-65 0.36739512569249655 1102 66-67 0.4861515164434991 1102 68-69 0.4330848151961213 1102 70-71 0.4370218732868629 1102 72-73 0.4007078659384824 1102 74-75 0.2235761222387147 1102 76-77 0.4037158224441306 1102 78-79 0.4437329843021445 1102 80-81 0.3490540732842966 1102 82-83 0.48354085221594545 1102 84-85 0.5300495326009447 1102 86-87 0.7432521365247489 1102 88-89 0.6793261303272047 1102 90-91 0.7419371435116098 1102 92-93 0.9022614578073345 1102 94-95 1.060558776686623 1102 96-97 1.013614831932653 1102 98-99 1.1884313908979678 1102 100-101 1.3672632593388023 1103 1 0.21370820247539868 1103 2 0.3114492114523877 1103 3 0.2532174483841274 1103 4 0.24004145033043756 1103 5 0.2894735094729981 1103 6 0.2928165185026046 1103 7 0.3625220863108609 1103 8 0.29622841424586 1103 9 0.4035806502722181 1103 10-11 0.3697779332180744 1103 12-13 0.33483464043047917 1103 14-15 0.4596938585179515 1103 16-17 0.4194311987299528 1103 18-19 0.431909176741776 1103 20-21 0.42387096818051617 1103 22-23 0.4245000191344843 1103 24-25 0.3742212915118657 1103 26-27 0.3674608130881438 1103 28-29 0.20305229912856504 1103 30-31 0.3983277364675786 1103 32-33 0.3792184377741421 1103 34-35 0.40344576257997744 1103 36-37 0.3822500129682638 1103 38-39 0.47444016191469274 1103 40-41 0.4958172087560513 1103 42-43 0.4489527719633699 1103 44-45 0.2616862291788138 1103 46-47 0.24577836189257596 1103 48-49 0.2514674753724293 1103 50-51 0.28945130107913286 1103 52-53 0.26794464970524245 1103 54-55 0.32592022118549124 1103 56-57 0.24792553114073002 1103 58-59 0.2646397205988009 1103 60-61 0.43080003618278795 1103 62-63 0.4356329202953759 1103 64-65 0.5091564973504319 1103 66-67 0.6164292638535755 1103 68-69 0.6134428902008828 1103 70-71 0.6077750268418036 1103 72-73 0.42666689198721386 1103 74-75 0.2102484023043658 1103 76-77 0.4143501968626708 1103 78-79 0.5041586662541029 1103 80-81 0.5410971888220928 1103 82-83 0.5973170890038837 1103 84-85 0.6461385090775202 1103 86-87 0.6365439316709178 1103 88-89 0.7578141181119094 1103 90-91 0.7225222643360425 1103 92-93 0.8415417602068835 1103 94-95 0.8028548933777486 1103 96-97 0.9151505044074746 1103 98-99 1.097715001969057 1103 100-101 1.268024108994073 1104 1 0.20110411072827006 1104 2 0.22855275405477826 1104 3 0.19209682536474304 1104 4 0.17711717148513628 1104 5 0.18682396565563408 1104 6 0.23144568632731932 1104 7 0.2996746099368153 1104 8 0.20110466396562998 1104 9 0.30296120934611537 1104 10-11 0.1704996359981692 1104 12-13 0.23699458617370084 1104 14-15 0.3902922883812465 1104 16-17 0.48032883998886433 1104 18-19 0.3923402040915178 1104 20-21 0.516168797697425 1104 22-23 0.42821001696336225 1104 24-25 0.4384631641343333 1104 26-27 0.3779338837796544 1104 28-29 0.5061065056904468 1104 30-31 0.5645355684809132 1104 32-33 0.38869696596722747 1104 34-35 0.3758001260706578 1104 36-37 0.524133031347823 1104 38-39 0.4607316280003104 1104 40-41 0.5735461186630744 1104 42-43 0.5128339699970539 1104 44-45 0.5621431543784396 1104 46-47 0.6006552834571721 1104 48-49 0.42366455645419165 1104 50-51 0.5881954463051002 1104 52-53 0.5136170850437125 1104 54-55 0.6694145356102794 1104 56-57 0.6811522886691037 1104 58-59 0.6400751644442408 1104 60-61 0.5910992405063524 1104 62-63 0.6855501419412278 1104 64-65 0.7528339071694106 1104 66-67 0.8799615976232502 1104 68-69 0.8330311271174189 1104 70-71 0.7263620028116868 1104 72-73 0.9019954771456646 1104 74-75 0.8147425491215508 1104 76-77 0.7587578666431618 1104 78-79 0.6665956808020148 1104 80-81 0.8029629938300964 1104 82-83 0.8007451948894584 1104 84-85 0.6634580321101282 1104 86-87 0.7168213965104506 1104 88-89 0.9019691742392375 1104 90-91 0.9524205611740584 1104 92-93 0.8773783873478571 1104 94-95 1.06498363723302 1104 96-97 1.0649103022033977 1104 98-99 1.2754279290413635 1104 100-101 1.497209472394939 1105 1 0.25568058159489837 1105 2 0.3188932124089554 1105 3 0.27175862632841685 1105 4 0.3046638340050265 1105 5 0.19992955862233686 1105 6 0.26891528976914714 1105 7 0.2350895299986604 1105 8 0.24698783187858453 1105 9 0.35067465009001353 1105 10-11 0.2516911247365101 1105 12-13 0.26178855723190253 1105 14-15 0.3309070904627447 1105 16-17 0.38118993025339876 1105 18-19 0.4423781082333349 1105 20-21 0.43270989192843956 1105 22-23 0.5284302722831811 1105 24-25 0.4283091220400337 1105 26-27 0.3721864974892668 1105 28-29 0.398428036482521 1105 30-31 0.5495286412208102 1105 32-33 0.39404601367320424 1105 34-35 0.38849491476527476 1105 36-37 0.5088741845126705 1105 38-39 0.541625348786944 1105 40-41 0.5335681653233308 1105 42-43 0.5888220553350436 1105 44-45 0.4797415079498606 1105 46-47 0.6157553160228986 1105 48-49 0.712675397799373 1105 50-51 0.7008980806359162 1105 52-53 0.7050211693945911 1105 54-55 0.7079361867490661 1105 56-57 0.7871650743798497 1105 58-59 0.6652344435359154 1105 60-61 0.8550184836968526 1105 62-63 1.065289709076204 1105 64-65 0.8807793308393954 1105 66-67 0.8694768274539086 1105 68-69 0.7192417947035885 1105 70-71 0.8663649156977904 1105 72-73 0.9174100394352287 1105 74-75 1.0603847700817148 1105 76-77 0.8884535573746639 1105 78-79 0.9514698153874974 1105 80-81 0.8977444062361712 1105 82-83 0.8924896981550461 1105 84-85 0.7455326831272124 1105 86-87 0.8748305374513201 1105 88-89 1.0197156229972357 1105 90-91 0.858201583692864 1105 92-93 1.155570702061258 1105 94-95 1.4771216674684418 1105 96-97 1.49265544382488 1105 98-99 1.3166370275002919 1105 100-101 1.1971164234314067 1106 1 -0.029199858766773445 1106 2 0.0023327044967444976 1106 3 -0.03562123545420093 1106 4 0.08494643263843216 1106 5 0.058181270299783705 1106 6 0.025369740130500418 1106 7 0.10951796836223338 1106 8 0.19309648673383606 1106 9 0.257562232752349 1106 10-11 -0.020528888828593495 1106 12-13 -0.15488954257700271 1106 14-15 0.004281240352526083 1106 16-17 -0.030719188725363722 1106 18-19 0.01730674537605381 1106 20-21 -0.03036738389221938 1106 22-23 0.11592710728389477 1106 24-25 -0.08808779407631562 1106 26-27 -0.07099911582744767 1106 28-29 0.047819153632751465 1106 30-31 0.09247574821095839 1106 32-33 0.0428958586598398 1106 34-35 0.29806007250540745 1106 36-37 0.12892103223065732 1106 38-39 0.09542976386070023 1106 40-41 0.13549131054782748 1106 42-43 0.21246862430395907 1106 44-45 0.11277108250521195 1106 46-47 0.11700348576538744 1106 48-49 0.23146360693106516 1106 50-51 0.16671737722126778 1106 52-53 0.20182881649115103 1106 54-55 0.21794102160770734 1106 56-57 0.13747829473941664 1106 58-59 0.1629358807033796 1106 60-61 0.08069847162116162 1106 62-63 0.20990260818118855 1106 64-65 0.33389165652097574 1106 66-67 0.3203260354307389 1106 68-69 0.18759724388715782 1106 70-71 -0.031356198886062 1106 72-73 0.26496971041278883 1106 74-75 0.24066456174742612 1106 76-77 0.30926369980822344 1106 78-79 0.3662585108693115 1106 80-81 0.19303604731551616 1106 82-83 0.036400210879200756 1106 84-85 0.18753500202624096 1106 86-87 0.33853128948952005 1106 88-89 0.44358242436016226 1106 90-91 0.351682043187747 1106 92-93 0.29515447458001276 1106 94-95 0.4864836207971628 1106 96-97 0.5813336398726712 1106 98-99 0.6377298573590977 1106 100-101 0.6793728072779359 1107 1 0.10409477741162476 1107 2 0.06017198922626221 1107 3 0.08481736686677976 1107 4 0.013264407301932124 1107 5 0.06756431698664755 1107 6 0.018241497947585117 1107 7 0.1314731126564297 1107 8 0.15431444315640874 1107 9 0.07889916525103047 1107 10-11 0.21208157128105398 1107 12-13 0.2474582340805611 1107 14-15 0.19409417896545733 1107 16-17 0.25144889361614986 1107 18-19 0.35836225858940907 1107 20-21 0.3980152117206259 1107 22-23 0.28501856784461665 1107 24-25 0.23417659751815023 1107 26-27 0.21613650756674474 1107 28-29 0.18826041432101448 1107 30-31 0.28288441561951316 1107 32-33 0.23322965343671598 1107 34-35 0.3645669071959645 1107 36-37 0.2847380488921232 1107 38-39 0.2645034784057856 1107 40-41 0.1793756517747198 1107 42-43 0.22851264856297604 1107 44-45 0.10446983575923241 1107 46-47 0.21418476803167152 1107 48-49 0.22362262537136957 1107 50-51 0.09438942519631865 1107 52-53 0.10641662108984207 1107 54-55 0.17840579753421792 1107 56-57 0.21030843779633557 1107 58-59 0.04255730375000866 1107 60-61 0.20746745243425124 1107 62-63 0.08403033141687644 1107 64-65 0.28933091636365305 1107 66-67 0.29049972013989844 1107 68-69 0.35430416313642255 1107 70-71 0.25931823454940783 1107 72-73 0.2330582736359723 1107 74-75 0.23688911459732154 1107 76-77 0.31508806940045986 1107 78-79 0.3526084311889939 1107 80-81 0.40231636507524726 1107 82-83 0.2666658637841053 1107 84-85 0.24506016106872153 1107 86-87 0.36898783578964967 1107 88-89 0.5029972159116447 1107 90-91 0.5913594474900599 1107 92-93 0.6709178714077844 1107 94-95 0.7376086899076384 1107 96-97 0.9271440954574324 1107 98-99 0.9048140385183459 1107 100-101 0.8762232783604169 1108 1 -0.07231193368393107 1108 2 -0.14589107359998366 1108 3 -0.014390716110185053 1108 4 0.01551785672037198 1108 5 0.02521100633354223 1108 6 0.058143518276779105 1108 7 0.0340937437274107 1108 8 0.02161037302941793 1108 9 0.03162916222115797 1108 10-11 0.05855488420310451 1108 12-13 0.07616709389436238 1108 14-15 0.09710896999426666 1108 16-17 0.2552775301879322 1108 18-19 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-0.4869401108190452 2316 74-75 -0.5267867314584258 2316 76-77 -0.7143569565255987 2316 78-79 -0.5549618579076245 2316 80-81 -0.6837762847106958 2316 82-83 -0.5764152211059113 2316 84-85 -0.7816606951013725 2316 86-87 -0.7722362317057545 2316 88-89 -0.7750825885730421 2316 90-91 -0.7576432972177436 2316 92-93 -0.8545385630156943 2316 94-95 -0.7023958129943253 2316 96-97 -0.9560176291883948 2316 98-99 -0.9818346852870548 2316 100-101 -0.9313222619620412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10209.0 3 2090.0 4 1967.0 5 2277.0 6 2643.0 7 3002.0 8 3765.0 9 4698.0 10 6104.0 11 7788.0 12 10338.0 13 12823.0 14 14764.0 15 18162.0 16 22415.0 17 29234.0 18 25910.0 19 18302.0 20 18269.0 21 19575.0 22 21542.0 23 23695.0 24 26634.0 25 30544.0 26 34677.0 27 40883.0 28 47508.0 29 57092.0 30 68460.0 31 84588.0 32 105288.0 33 134507.0 34 177408.0 35 243034.0 36 338375.0 37 438666.0 38 308552.0 39 28292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3013207795839 19.517886095745332 14.845416358221996 22.335376766448775 2 14.782531969729304 20.21455422952542 41.6788827420917 23.324031058653578 3 18.955142108470696 25.153209572802503 30.268622047486453 25.623026271240345 4 12.163325140427823 16.749231838158856 38.00614857791051 33.08129444350281 5 13.048269736561277 38.09822064758052 34.42061047647505 14.432899139383155 6 31.193198451480008 36.56894251435235 17.84631570488246 14.391543329285184 7 28.01717899570525 33.48112462971884 20.8025747993563 17.699121575219607 8 26.150087311554444 35.54394134893994 19.78378551247311 18.522185827032512 9 26.33324900995194 16.96818767186697 20.886600905362513 35.81196241281857 10-11 24.47509078649096 27.290522716560606 27.5229295728909 20.711456924057533 12-13 25.376432917329485 24.727192220017383 28.345769716450462 21.550605146202667 14-15 22.38067026371031 25.79658390186303 26.939615586460246 24.883130247966413 16-17 21.21396258706321 29.490415512965125 27.423490777973086 21.87213112199858 18-19 21.104708168422427 27.581173886188974 30.403254191813723 20.910863753574876 20-21 21.8572885095588 26.679815403864517 30.52420169796632 20.93869438861037 22-23 21.70657470568859 26.759673028544512 30.39232024164354 21.141432024123358 24-25 21.389972581084226 26.888227740989638 30.642442251368486 21.079357426557646 26-27 21.290011677513686 27.185424036000438 30.303855178332668 21.22070910815321 28-29 21.004512571731276 27.086726167339904 30.701150160477976 21.207611100450848 30-31 21.124250965431447 26.85890144603548 30.743486771805546 21.27336081672753 32-33 20.96833584076439 27.076113961613956 30.50807565019963 21.447474547422022 34-35 21.450911240259778 27.03148260111617 30.317064824934807 21.200541333689245 36-37 21.523811280651213 26.741273877125614 30.2224996080047 21.512415234218473 38-39 21.226220974320086 26.986965343389418 30.418379685610642 21.36843399667985 40-41 21.373920322229832 26.970823131451883 30.405972368939388 21.2492841773789 42-43 21.367296236927228 26.88750138363336 30.176736101146673 21.56846627829274 44-45 21.251091340959004 27.233331003226002 29.905683951758167 21.609893704056827 46-47 21.456754453560766 27.35263976705508 29.5015460029934 21.68905977639075 48-49 21.618328372405763 27.498934015563375 29.18759686108583 21.695140750945036 50-51 21.62984013248286 27.67834775274841 28.90930426407682 21.782507850691914 52-53 21.7564681158921 27.766184849245445 28.909721982981363 21.56762505188109 54-55 21.50207817428131 27.641593494475114 28.923590378392316 21.932737952851262 56-57 21.587171398139958 28.061500852470626 28.834534621128604 21.51679312826081 58-59 21.8111679213281 27.607429104046314 28.866168900955934 21.715234073669652 60-61 21.702957497993218 27.5275585645754 29.205305491178862 21.564178446252523 62-63 21.604936247446886 27.634119738472506 29.251369141772216 21.50957487230839 64-65 21.637121051120427 27.531392963688823 29.124255535947512 21.707230449243234 66-67 21.443675379412475 27.84426658534716 28.86699055111591 21.845067484124456 68-69 21.315562456689996 27.822234118845213 29.06341353237677 21.79878989208802 70-71 21.44010401402484 27.748946476799535 29.02331912786601 21.78763038130961 72-73 21.497084968734733 27.817993350187393 28.7474054910139 21.937516190063974 74-75 21.391023372138992 28.061855961679523 28.586790936596877 21.960329729584604 76-77 21.527779485754248 27.898073976423365 28.67270962405115 21.901436913771235 78-79 21.566290865899486 27.88101452884504 28.556472084469604 21.99622252078587 80-81 21.37697763050783 28.164003866109322 28.57022817095155 21.888790332431295 82-83 21.607515080022026 28.030345105174355 28.616494950577614 21.745644864226005 84-85 21.508125218886732 27.93885086029017 28.471392413675378 22.08163150714772 86-87 21.653589293289045 27.631523402999637 28.729428495900557 21.98545880781077 88-89 21.790514093195018 27.634712254542325 28.633757269973724 21.94101638228893 90-91 21.592283885020688 27.639553922606435 28.79805330458024 21.970108887792637 92-93 21.65391395371808 27.622933652694222 28.642228509434712 22.080923884152988 94-95 21.47961030225799 27.60438932557075 28.530288305665735 22.38571206650553 96-97 21.59902810490909 27.529076004925873 28.454142901614272 22.417752988550763 98-99 21.51778308770861 27.65108794716976 28.35770178961156 22.473427175510068 100-101 21.673052961959044 27.058768327772775 28.99672431304724 22.271454397220943 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3229.0 1 1855.0 2 534.5 3 633.0 4 568.5 5 500.5 6 837.5 7 1921.0 8 3259.0 9 3886.5 10 3576.0 11 3169.0 12 3314.0 13 3558.5 14 3804.5 15 4037.0 16 4138.0 17 4383.5 18 4641.5 19 4818.5 20 5280.5 21 6114.0 22 7479.0 23 9149.0 24 11068.0 25 13661.5 26 17064.5 27 20843.5 28 23607.5 29 26320.0 30 31148.0 31 36159.5 32 41030.5 33 47507.0 34 54942.5 35 60448.0 36 66405.0 37 72119.0 38 75202.5 39 76805.5 40 78219.5 41 82747.0 42 87414.5 43 91370.0 44 95942.5 45 99469.0 46 97791.0 47 95156.5 48 96485.5 49 93848.0 50 87602.5 51 81110.5 52 74979.5 53 71726.0 54 68832.5 55 62925.5 56 55547.5 57 48813.0 58 43183.5 59 37906.0 60 31830.0 61 25435.5 62 19488.0 63 14904.0 64 11571.0 65 8937.5 66 6510.0 67 4844.5 68 3512.5 69 2299.5 70 1673.5 71 1231.5 72 921.0 73 708.0 74 523.0 75 382.5 76 270.0 77 175.0 78 107.5 79 78.5 80 54.0 81 35.5 82 25.0 83 13.5 84 15.0 85 13.0 86 7.0 87 6.0 88 4.5 89 5.0 90 5.5 91 2.5 92 3.0 93 3.5 94 2.0 95 1.5 96 2.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3466744132761612 2 0.30277241334162547 3 0.3021586854767438 4 0.3035498019704756 5 0.3145559883473536 6 0.20891296520572158 7 0.20682629046512388 8 0.20919937154266635 9 0.2069081208471081 10-11 0.21494795587705803 12-13 0.20457595496055775 14-15 0.20621256260024223 16-17 0.21239075644005107 18-19 0.20500556446597495 20-21 0.19676115348106443 22-23 0.19575873130175775 24-25 0.19999345356944126 26-27 0.1954314097738208 28-29 0.2093221171156427 30-31 0.21578671729239632 32-33 0.21803705279696248 34-35 0.21637998756178192 36-37 0.18974419822591732 38-39 0.18125429609505417 40-41 0.1873506595528788 42-43 0.19843867631174103 44-45 0.20431000621910902 46-47 0.20656034172367518 48-49 0.2053942587804 50-51 0.20960852345258746 52-53 0.23779909004615235 54-55 0.1963724591666394 56-57 0.18029278910673957 58-59 0.17912670616346435 60-61 0.17309171549212793 62-63 0.18055873784818827 64-65 0.1768149978724101 66-67 0.176794540276914 68-69 0.18598000065464304 70-71 0.18039507708421984 72-73 0.176426303557985 74-75 0.18184756636443977 76-77 0.1860004582501391 78-79 0.18913047036103564 80-81 0.18076331380314883 82-83 0.17779696245622073 84-85 0.17088229517855388 86-87 0.16836601093253903 88-89 0.17388956171647407 90-91 0.18242037903832936 92-93 0.17213020850381328 94-95 0.17769467447874046 96-97 0.17636493077149684 98-99 0.18070194101666065 100-101 0.15664380871329908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.13298246871731 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98260391581681 36.349859811858835 2 13.913443144228093 13.672179161697077 3 4.932677864701958 7.270715251506936 4 2.2844651741593234 4.48970349409461 5 1.244375393058336 3.056993718581922 6 0.7721685672389486 2.276336680622749 7 0.5149699947246567 1.7711408205905401 8 0.36284127022537943 1.4261979015128547 9 0.27802330039658085 1.229410254985212 >10 1.491385868832016 13.725082588379372 >50 0.13944558578660343 4.766966533705445 >100 0.07918252909078709 7.048933503988889 >500 0.003500574586757842 1.1227091550668038 >1k 6.667761117631425E-4 0.6125431697360714 >5k 1.6669402794078562E-4 0.6684820632953355 >10k+ 8.334701397039281E-5 0.5127458903774135 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12521 0.5122991064122288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9826 0.4020326666884881 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6498 0.2658669110667409 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3434 0.14050276586691107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.091519099211155E-5 0.0 10-11 0.0 0.0 0.0 4.091519099211155E-5 0.0 12-13 0.0 0.0 0.0 4.091519099211155E-5 0.0 14-15 0.0 0.0 0.0 4.091519099211155E-5 0.0 16-17 0.0 0.0 0.0 4.091519099211155E-5 4.091519099211155E-5 18-19 0.0 0.0 0.0 4.091519099211155E-5 4.091519099211155E-5 20-21 0.0 0.0 0.0 4.091519099211155E-5 4.091519099211155E-5 22-23 0.0 0.0 0.0 1.0228797748027888E-4 4.091519099211155E-5 24-25 0.0 0.0 0.0 1.636607639684462E-4 4.091519099211155E-5 26-27 0.0 0.0 0.0 2.2503355045661353E-4 4.091519099211155E-5 28-29 0.0 0.0 0.0 5.728126738895618E-4 4.091519099211155E-5 30-31 0.0 0.0 0.0 0.0015956924486923503 4.091519099211155E-5 32-33 0.0 0.0 0.0 0.002945893751432032 4.091519099211155E-5 34-35 0.0 0.0 0.0 0.004582501391116494 4.091519099211155E-5 36-37 0.0 0.0 0.0 0.006812379300186574 4.091519099211155E-5 38-39 0.0 0.0 0.0 0.008837681254296095 4.091519099211155E-5 40-41 0.0 0.0 0.0 0.011394880691303066 4.091519099211155E-5 42-43 0.0 0.0 0.0 0.015465942195018166 4.091519099211155E-5 44-45 0.0 0.0 0.0 0.021766881607803344 4.091519099211155E-5 46-47 0.0 0.0 0.0 0.03449150600635004 6.137278648816733E-5 48-49 0.0 0.0 0.0 0.05848826552322346 8.18303819842231E-5 50-51 0.0 0.0 0.0 0.07853670910935812 8.18303819842231E-5 52-53 0.0 0.0 0.0 0.0991784229648784 8.18303819842231E-5 54-55 0.0 0.0 0.0 0.12644839776112074 8.18303819842231E-5 56-57 0.0 0.0 0.0 0.1499746325815849 8.18303819842231E-5 58-59 0.0 0.0 0.0 0.1761603548165363 8.18303819842231E-5 60-61 0.0 0.0 0.0 0.21543893816896337 8.18303819842231E-5 62-63 0.0 0.0 0.0 0.2482733789401329 8.18303819842231E-5 64-65 0.0 0.0 0.0 0.28716326797813496 8.18303819842231E-5 66-67 0.0 0.0 0.0 0.34669487087165723 8.18303819842231E-5 68-69 0.0 0.0 0.0 0.4167212202546562 8.18303819842231E-5 70-71 0.0 0.0 0.0 0.4799556479329645 8.18303819842231E-5 72-73 0.0 0.0 0.0 0.5548713626395207 8.18303819842231E-5 74-75 0.0 0.0 0.0 0.629807534941573 8.18303819842231E-5 76-77 0.0 0.0 0.0 0.7035776243003502 8.18303819842231E-5 78-79 0.0 0.0 0.0 0.790051880462178 8.18303819842231E-5 80-81 0.0 0.0 0.0 0.9153955680665118 1.0228797748027888E-4 82-83 0.0 0.0 0.0 1.0334768092697457 1.2274557297633465E-4 84-85 0.0 0.0 0.0 1.164487250826487 1.2274557297633465E-4 86-87 0.0 0.0 0.0 1.3726432849988544 1.2274557297633465E-4 88-89 0.0 0.0 0.0 1.641722038558476 1.2274557297633465E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6355 0.0 52.393745 1 GTATCAA 10995 0.0 38.80558 1 ATCAACG 13925 0.0 30.321121 3 TATCAAC 13990 0.0 30.316189 2 TCAACGC 14080 0.0 29.95479 4 CAACGCA 14275 0.0 29.446276 5 AACGCAG 14655 0.0 28.752329 6 GTACATG 11485 0.0 27.375835 1 TACATGG 11785 0.0 26.224743 2 ACATGGG 11710 0.0 25.296394 3 ACGCAGA 16840 0.0 24.825762 7 CGCAGAG 16855 0.0 24.549992 8 GCAGAGT 17690 0.0 22.880936 9 GAGTACT 9800 0.0 22.296684 12-13 CATGGGA 6755 0.0 21.750961 4 GTACTTT 10555 0.0 20.633024 14-15 TGGTATC 3095 0.0 20.432356 2 GTCTAGC 420 0.0 20.385443 1 CAGAGTA 17365 0.0 20.339138 10-11 AGTACTT 10355 0.0 20.230053 12-13 >>END_MODULE