FastQCFastQC Report
Thu 26 May 2016
SRR937735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444080
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141050.5771087689437334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.49184151091617295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84200.3445059081535793No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.11926778174200517No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA28990.11861313868613138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70300.054.075881
GTATCAA122750.039.9896621
ATCAACG161200.030.0949783
TATCAAC162200.030.0266132
TCAACGC162450.029.8341624
CAACGCA165050.029.3641875
AACGCAG169200.028.6614916
ACGCAGA195450.024.6434967
CGCAGAG195350.024.5831648
GTACATG117950.024.3739761
TACATGG120150.023.4907042
GAGTACT124100.022.75517312-13
GCAGAGT207400.022.7426229
ACATGGG118050.022.6205793
GTACTTT130250.021.64294814-15
AGTACTT127000.021.5810312-13
AGAGTAC194500.020.24580210-11
CAGAGTA204600.020.14020710-11
TGGTATC33050.019.839972
CATGGGG50200.019.4983064
Produced by FastQC (version 0.11.5)