##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937735_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444080 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10032404831266 31.0 31.0 34.0 31.0 34.0 2 32.38464902949167 34.0 31.0 34.0 31.0 34.0 3 32.47517348040981 34.0 31.0 34.0 31.0 34.0 4 36.12585512749173 37.0 35.0 37.0 35.0 37.0 5 36.03446327452457 37.0 35.0 37.0 35.0 37.0 6 36.075892360315535 37.0 35.0 37.0 35.0 37.0 7 36.01700926319924 37.0 35.0 37.0 35.0 37.0 8 35.99138121501751 37.0 35.0 37.0 35.0 37.0 9 37.67504377925436 39.0 37.0 39.0 35.0 39.0 10-11 37.58990213086315 39.0 37.0 39.0 35.0 39.0 12-13 37.481436982422835 39.0 37.0 39.0 35.0 39.0 14-15 38.67786754116068 40.0 38.0 41.0 34.0 41.0 16-17 38.63243960917809 40.0 38.0 41.0 34.0 41.0 18-19 38.62505830414716 40.0 38.0 41.0 34.0 41.0 20-21 38.535377319891325 40.0 38.0 41.0 34.0 41.0 22-23 38.47656173284017 40.0 38.0 41.0 34.0 41.0 24-25 38.50182093057511 40.0 38.0 41.0 34.0 41.0 26-27 38.37149131779647 40.0 38.0 41.0 34.0 41.0 28-29 38.21957423652253 40.0 38.0 41.0 34.0 41.0 30-31 38.25546606494059 40.0 38.0 41.0 34.0 41.0 32-33 38.182136427612846 40.0 37.5 41.0 34.0 41.0 34-35 38.040328876305196 40.0 37.0 41.0 33.5 41.0 36-37 37.88729767438055 40.0 37.0 41.0 33.0 41.0 38-39 37.763717636083925 40.0 37.0 41.0 33.0 41.0 40-41 37.577714313770414 40.0 37.0 41.0 33.0 41.0 42-43 37.35016325161206 40.0 37.0 41.0 32.5 41.0 44-45 37.03395735818795 40.0 36.0 41.0 31.5 41.0 46-47 36.643191098491044 39.5 36.0 41.0 30.5 41.0 48-49 36.29634340938104 39.0 36.0 41.0 30.0 41.0 50-51 36.03904884455501 39.0 35.5 41.0 29.5 41.0 52-53 35.80105315701614 39.0 35.0 40.0 28.5 41.0 54-55 35.571280399986904 39.0 35.0 40.0 28.0 41.0 56-57 35.308887597787304 38.0 35.0 40.0 27.5 41.0 58-59 35.05129782985827 38.0 34.0 40.0 27.0 41.0 60-61 34.73029872180943 38.0 34.0 40.0 26.0 41.0 62-63 34.35857889267127 37.5 34.0 40.0 26.0 41.0 64-65 33.9374691090308 37.0 33.0 39.5 25.5 41.0 66-67 33.509891247422345 36.0 33.0 39.0 24.5 40.5 68-69 33.059275064646 36.0 32.0 39.0 23.0 40.0 70-71 32.66486592091912 35.0 32.0 38.5 22.0 40.0 72-73 32.1726494222775 35.0 31.5 37.5 21.5 39.5 74-75 31.683307420379037 35.0 31.0 37.0 20.0 39.0 76-77 30.444452104677424 33.5 29.5 35.5 19.5 38.5 78-79 31.04779896730058 34.0 31.0 36.0 19.5 38.5 80-81 30.854035260711598 34.0 31.0 36.0 18.0 37.5 82-83 30.470867156557887 34.0 30.5 35.0 17.5 37.0 84-85 30.076003854210988 34.0 30.0 35.0 12.0 36.5 86-87 29.672355855782136 34.0 30.0 35.0 7.0 36.0 88-89 29.21452612025793 34.0 29.0 35.0 4.0 36.0 90-91 28.648903677457366 34.0 29.0 35.0 2.0 35.0 92-93 28.176375159569243 33.0 28.5 35.0 2.0 35.0 94-95 27.67062923472227 33.0 27.0 35.0 2.0 35.0 96-97 27.061005777224967 33.0 26.0 34.5 2.0 35.0 98-99 26.111520694903604 32.0 24.5 34.0 2.0 35.0 100-101 23.288794352067036 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24451897391185895 1101 2 0.22182738959472204 1101 3 0.261898938517632 1101 4 0.12208899123769612 1101 5 0.00793022789653719 1101 6 0.014174812514951896 1101 7 0.05308822403346625 1101 8 0.07585309250774941 1101 9 0.07719997560795377 1101 10-11 0.11507239773808209 1101 12-13 0.16416750549824854 1101 14-15 0.20601513086874945 1101 16-17 0.2279378451852736 1101 18-19 0.2622411741454087 1101 20-21 0.22977978455575254 1101 22-23 0.2051225005615933 1101 24-25 0.18704987446307797 1101 26-27 0.006111079226194249 1101 28-29 -0.02617626546004459 1101 30-31 0.1265289797011846 1101 32-33 0.19362182752458068 1101 34-35 0.1608385724805501 1101 36-37 0.024540144053894153 1101 38-39 0.036680389837975724 1101 40-41 0.03216066846114529 1101 42-43 0.16134178155054713 1101 44-45 0.08401202282576037 1101 46-47 0.1479056945195012 1101 48-49 0.059256664904793865 1101 50-51 0.1904360505203755 1101 52-53 0.18235561101877096 1101 54-55 0.06227165746022223 1101 56-57 0.0781291937936075 1101 58-59 -0.12425503936731275 1101 60-61 0.11314117388523215 1101 62-63 0.06226996838017129 1101 64-65 0.10281099159158202 1101 66-67 0.020104398016393077 1101 68-69 0.10547837491862566 1101 70-71 -5.140660695914789E-4 1101 72-73 0.049443590800635207 1101 74-75 0.024611279783908202 1101 76-77 -0.19168635576727766 1101 78-79 -0.10798669000886463 1101 80-81 -0.08636619773756848 1101 82-83 -0.12296955965374323 1101 84-85 -0.09402875209761419 1101 86-87 -0.1574901201832688 1101 88-89 -0.11536698513274146 1101 90-91 -0.0675711204870133 1101 92-93 -0.3640060253565842 1101 94-95 -0.35619608422493343 1101 96-97 -0.23720864896271365 1101 98-99 -0.2726612410345375 1101 100-101 -0.2169571417843521 1102 1 0.31953535407081546 1102 2 0.12361501621783333 1102 3 0.1926538607422117 1102 4 0.08886443693100432 1102 5 -0.026115180752817935 1102 6 -0.01349789661034606 1102 7 -0.025996555026942758 1102 8 0.05235534563686173 1102 9 0.09443053122505063 1102 10-11 0.06966849625326432 1102 12-13 0.18050282344215418 1102 14-15 0.2235938637777295 1102 16-17 0.17611587562019082 1102 18-19 0.21108970963786788 1102 20-21 0.15818134481205703 1102 22-23 0.21610696657895545 1102 24-25 0.1322821139921757 1102 26-27 0.11792030339222492 1102 28-29 0.05508936954675647 1102 30-31 0.1261945184844464 1102 32-33 0.19490744579777441 1102 34-35 0.08460040655190681 1102 36-37 0.04735366979210909 1102 38-39 0.16273850590339833 1102 40-41 0.25435000727246404 1102 42-43 0.3553535541633437 1102 44-45 0.22446357712084364 1102 46-47 0.08815055811313499 1102 48-49 0.19004524426758707 1102 50-51 0.16513463976929899 1102 52-53 0.24261243925024445 1102 54-55 0.047290538165192686 1102 56-57 -0.02489804949684782 1102 58-59 0.01870339142928401 1102 60-61 -0.04598639932959969 1102 62-63 -0.06087031647106045 1102 64-65 -0.05826795708180299 1102 66-67 -0.06311050228357118 1102 68-69 -0.023295524023446035 1102 70-71 -0.126793221961222 1102 72-73 -0.15914203918397618 1102 74-75 -0.28142864665572986 1102 76-77 -0.2879267795728957 1102 78-79 -0.14796693886066947 1102 80-81 -0.07791076495847093 1102 82-83 0.05842553400067274 1102 84-85 -0.04532560700158683 1102 86-87 -0.381457800889919 1102 88-89 -0.39926652859845646 1102 90-91 -0.2557598916638426 1102 92-93 -0.2625825077741588 1102 94-95 -0.5757065680542119 1102 96-97 -0.631042007090489 1102 98-99 -0.3270544471459971 1102 100-101 -0.16914343140666332 1103 1 0.28162536296875373 1103 2 0.18002895202381808 1103 3 0.18232515834237262 1103 4 0.13007209480423398 1103 5 0.04978371724959629 1103 6 0.09497223014000866 1103 7 0.055858965350353174 1103 8 0.11991103376452372 1103 9 0.13418541441156862 1103 10-11 0.14990276757446708 1103 12-13 0.2522083241628934 1103 14-15 0.23313043980224535 1103 16-17 0.19623411899795684 1103 18-19 0.18947923367424124 1103 20-21 0.08860707744388918 1103 22-23 0.05756520021171241 1103 24-25 0.021433580921019768 1103 26-27 0.0687906577676003 1103 28-29 0.11218828000194492 1103 30-31 0.09423181018112103 1103 32-33 0.03659294244964428 1103 34-35 0.03289897470077108 1103 36-37 0.012063103994798041 1103 38-39 0.06068430595393437 1103 40-41 -0.006385473487647175 1103 42-43 0.06846429185230107 1103 44-45 0.08010587569913241 1103 46-47 0.01594701906537921 1103 48-49 -0.15102767755002589 1103 50-51 -0.20527794505613883 1103 52-53 -0.03582218987084218 1103 54-55 -0.17745846977634727 1103 56-57 -0.09423607626885655 1103 58-59 -0.06800602974143288 1103 60-61 -0.04618984280959637 1103 62-63 -0.24780837625737462 1103 64-65 -0.19875002400964803 1103 66-67 -0.04291211231065262 1103 68-69 -0.15358065621465045 1103 70-71 -0.21211734507276958 1103 72-73 -0.1854317875873832 1103 74-75 -0.2796193752802232 1103 76-77 -0.1367606719534713 1103 78-79 -0.07913114960494738 1103 80-81 -0.05126480946553258 1103 82-83 0.07277307772835329 1103 84-85 0.03317106876091103 1103 86-87 -0.025986365874910433 1103 88-89 -0.047623088999085184 1103 90-91 -0.059440046657364576 1103 92-93 -0.1397124922299149 1103 94-95 -0.3630702663525973 1103 96-97 -0.20598037674801617 1103 98-99 -0.1560106807338677 1103 100-101 -0.2127793066733652 1104 1 0.24919293818177835 1104 2 0.14433772624647645 1104 3 0.16545591278167393 1104 4 0.06014195959369317 1104 5 0.02010585623378347 1104 6 0.0333322357127841 1104 7 0.05740265259031929 1104 8 0.1165535069455359 1104 9 0.10036573614744526 1104 10-11 0.13995324080074312 1104 12-13 0.15965144542663978 1104 14-15 0.19059396393243588 1104 16-17 0.14496769150805022 1104 18-19 0.1415610686168165 1104 20-21 0.12305355156689757 1104 22-23 0.12352246946661438 1104 24-25 0.07745221436037042 1104 26-27 0.061465003266981455 1104 28-29 -0.06154923517645017 1104 30-31 0.07221722336922909 1104 32-33 0.0790319654599756 1104 34-35 0.12338126734647403 1104 36-37 0.09106639744883438 1104 38-39 0.09749051125415775 1104 40-41 -0.02021493947002284 1104 42-43 0.17801403559654716 1104 44-45 0.10782640522600673 1104 46-47 0.0717594315045531 1104 48-49 0.03699460223868556 1104 50-51 -0.040274129684753746 1104 52-53 0.14814384448376927 1104 54-55 0.02166841483677473 1104 56-57 0.1895366789384738 1104 58-59 0.23511062302865327 1104 60-61 0.2522644897762163 1104 62-63 0.15857938287140172 1104 64-65 0.04341714936945351 1104 66-67 0.11113009510269478 1104 68-69 0.18707111812752686 1104 70-71 0.138701563045025 1104 72-73 0.0613058400366171 1104 74-75 -0.04359604599917688 1104 76-77 -0.164726035877667 1104 78-79 -0.034111818154194395 1104 80-81 0.08758592570950441 1104 82-83 0.042458166380040296 1104 84-85 0.17903862197986342 1104 86-87 0.1591636712179998 1104 88-89 0.2071462666734405 1104 90-91 0.1782808576881969 1104 92-93 0.17392256307705622 1104 94-95 0.09496779118136445 1104 96-97 0.13925196487164015 1104 98-99 0.3337856229880849 1104 100-101 0.29800245903383527 1105 1 0.2344866664055658 1105 2 0.18565732563166648 1105 3 0.12204376813124895 1105 4 0.03981612764637532 1105 5 -0.017955339165098394 1105 6 0.14246038692593999 1105 7 0.09134988934879829 1105 8 0.07918489670209539 1105 9 0.14028481200193 1105 10-11 0.12276009954887002 1105 12-13 0.18593721420320009 1105 14-15 0.17989248799501212 1105 16-17 0.0998299294609879 1105 18-19 0.18952713321190373 1105 20-21 0.06212162239116026 1105 22-23 0.06341236011787998 1105 24-25 0.08513861371761777 1105 26-27 0.0991209581752841 1105 28-29 -0.008554568518796657 1105 30-31 0.09183434223751163 1105 32-33 0.0682642875654551 1105 34-35 0.08028428626840167 1105 36-37 0.07032652155918839 1105 38-39 0.1714202791472772 1105 40-41 0.120091938997831 1105 42-43 0.21477844734003781 1105 44-45 0.17783025620794035 1105 46-47 0.01244487248282411 1105 48-49 -0.017234660491247666 1105 50-51 0.07423215583893494 1105 52-53 0.12804739527503983 1105 54-55 -0.07197052081604483 1105 56-57 -0.08010001880649753 1105 58-59 7.740765058557031E-4 1105 60-61 0.01444043823898511 1105 62-63 -0.10725035020229967 1105 64-65 0.03598724738215964 1105 66-67 -0.07740540210499347 1105 68-69 -0.0732344841919641 1105 70-71 -0.13912051370191136 1105 72-73 -0.08693745581796009 1105 74-75 -0.16157406075179281 1105 76-77 -0.14498686595442578 1105 78-79 -0.0474212286356277 1105 80-81 -0.0918853967295199 1105 82-83 -0.02455842692922161 1105 84-85 0.012529713168408563 1105 86-87 -0.02501193412142655 1105 88-89 0.037063649448082714 1105 90-91 0.16171297279283436 1105 92-93 0.26124371919422984 1105 94-95 0.14370271403777224 1105 96-97 0.0025618260897992684 1105 98-99 -0.02360183931905624 1105 100-101 -0.05764515949865867 1106 1 0.05648639029159597 1106 2 -0.024306722267802172 1106 3 0.0017766593767163386 1106 4 -0.01372549223749786 1106 5 -0.0600181797247501 1106 6 -0.10140581580816388 1106 7 -0.1133800230738089 1106 8 -0.14037812144123052 1106 9 -0.08118164530695537 1106 10-11 -0.23226968581548846 1106 12-13 -0.30478683165691933 1106 14-15 -0.1077215951920536 1106 16-17 -0.07452930855613005 1106 18-19 -0.02033215464320648 1106 20-21 0.012706316626449166 1106 22-23 -0.008549842057114176 1106 24-25 0.009617505893395162 1106 26-27 0.07589960184849076 1106 28-29 0.09863622393974225 1106 30-31 -0.01013269766037439 1106 32-33 -0.0398760046799822 1106 34-35 -0.2733248297315427 1106 36-37 0.003004623659485617 1106 38-39 0.05894372305603923 1106 40-41 -0.16487242396944168 1106 42-43 -0.26326454910370245 1106 44-45 -0.13922727116286637 1106 46-47 -0.09668387338691531 1106 48-49 -0.10132568415226473 1106 50-51 0.0069852297549815034 1106 52-53 0.08277451505120581 1106 54-55 -0.095274296015738 1106 56-57 0.001177807408986098 1106 58-59 0.13720924758194286 1106 60-61 0.1771393033074702 1106 62-63 -0.0022830771542388106 1106 64-65 -0.3568085697776908 1106 66-67 -0.4353763454065316 1106 68-69 0.042868359094455855 1106 70-71 -0.0925963846638922 1106 72-73 -0.18424278115124082 1106 74-75 -0.06003895016872107 1106 76-77 -0.12149111421410552 1106 78-79 -0.2841709089850184 1106 80-81 -0.41558541265476734 1106 82-83 -0.48674835724768073 1106 84-85 -0.6324060588279039 1106 86-87 -0.3289328526758304 1106 88-89 -0.32556657380323273 1106 90-91 -0.44479634232995835 1106 92-93 -0.693501875012192 1106 94-95 -0.3986809228299748 1106 96-97 -0.4169907661735124 1106 98-99 -0.7045028874064236 1106 100-101 -0.9095558452236787 1107 1 0.2064642607980005 1107 2 0.07454409949291119 1107 3 0.02988069203503585 1107 4 -0.061424802617430885 1107 5 -0.07688650994487034 1107 6 -0.07441660057797606 1107 7 0.0012079087871512684 1107 8 0.022966528806719566 1107 9 0.04422258913275812 1107 10-11 0.049122379523787174 1107 12-13 0.0660399205676967 1107 14-15 0.048186201884021784 1107 16-17 -0.10581822076071745 1107 18-19 0.05777003978241879 1107 20-21 -0.06418243211683716 1107 22-23 -0.06433907312437981 1107 24-25 -0.06582476582284613 1107 26-27 -0.014934333623891405 1107 28-29 -0.03153207333359376 1107 30-31 0.005822030910422882 1107 32-33 -0.01981600759287261 1107 34-35 -0.028862010815849715 1107 36-37 -0.003697502044509804 1107 38-39 0.022822309200577706 1107 40-41 0.08660473740335561 1107 42-43 0.08279237106467718 1107 44-45 -0.13527033388477605 1107 46-47 -0.16298426040560088 1107 48-49 -0.23399687664208813 1107 50-51 -0.12600970379290288 1107 52-53 -0.10543378782166002 1107 54-55 -0.03885016765619298 1107 56-57 -0.06114916893861988 1107 58-59 -0.15935113624103536 1107 60-61 -0.1677501269959336 1107 62-63 -0.2776496985249608 1107 64-65 -0.14348477640070456 1107 66-67 -0.12093299761715315 1107 68-69 0.03237657528759286 1107 70-71 -0.19944139760929147 1107 72-73 -0.040651708740426784 1107 74-75 -0.1302690544813565 1107 76-77 -0.08849080737065051 1107 78-79 -0.1345394039390726 1107 80-81 -0.16959777971872825 1107 82-83 -0.1497366024566098 1107 84-85 -0.08796968218251067 1107 86-87 -0.10025253406768186 1107 88-89 -0.15335952092858562 1107 90-91 -0.00721940168649482 1107 92-93 -0.06508300965424851 1107 94-95 -0.2038946456612436 1107 96-97 -0.006082976167554932 1107 98-99 0.08647004673818515 1107 100-101 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-0.4681207309302451 2316 84-85 -0.3206977876810342 2316 86-87 -0.4705692152570684 2316 88-89 -0.4535948061496171 2316 90-91 -0.29195911848489686 2316 92-93 -0.03886073816363478 2316 94-95 -0.10628109776978789 2316 96-97 -0.02046613875153369 2316 98-99 0.10741807946843451 2316 100-101 0.2127032243279139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 9.0 4 12.0 5 48.0 6 93.0 7 246.0 8 538.0 9 1110.0 10 1867.0 11 3325.0 12 5008.0 13 7227.0 14 9555.0 15 13340.0 16 18973.0 17 26908.0 18 24131.0 19 14174.0 20 11733.0 21 11951.0 22 12435.0 23 13687.0 24 15954.0 25 19265.0 26 23624.0 27 29137.0 28 36801.0 29 46374.0 30 57420.0 31 72972.0 32 93603.0 33 123086.0 34 167509.0 35 239306.0 36 367112.0 37 543765.0 38 402746.0 39 29029.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.377172686253346 20.333200471101645 15.4589026016171 21.830724241027912 2 15.3656590618965 21.034949756145462 40.51201270007528 23.08737848188275 3 19.133088933259142 25.344424077771592 30.604399201335468 24.91808778763379 4 12.716114038820333 17.44963339988871 37.44128670092632 32.39296586036463 5 13.118366892504774 38.498711445702256 34.224378726901804 14.15854293489117 6 31.41885699322444 37.1068459297568 17.202832967824293 14.271464109194461 7 28.039875945140913 33.822665379201986 20.532838532290267 17.604620143366827 8 26.526382115151716 35.891746587673076 19.336028280580013 18.245843016595202 9 26.378391869333246 17.037576511407153 20.719984615888187 35.86404700337141 10-11 24.6690983928513 27.658894144217864 27.0861019279238 20.585905535007036 12-13 25.523945250813068 24.986563932664794 28.23982096427489 21.249669852247244 14-15 22.414200844489542 26.090328467153284 26.874202153775656 24.62126853458152 16-17 21.181651173447676 29.711056921213707 27.415223724264344 21.69206818107427 18-19 21.10254165166443 27.691646754607053 30.543230990802268 20.662580602926255 20-21 21.89770793100062 26.760417007626593 30.809016071487022 20.532858989885767 22-23 21.730554188295628 26.79632382334156 30.685936234815742 20.787185753547067 24-25 21.528667684638283 26.934186989275293 30.890917467378266 20.64622785870816 26-27 21.356445799572757 27.26692468113364 30.634880888230963 20.741748631062634 28-29 21.01347312310586 27.221301846810892 30.921592880017645 20.84363215006561 30-31 21.15675427972898 26.93868449477922 31.001583417891393 20.902977807600408 32-33 21.017233478445878 27.135179699518837 30.787167359497236 21.060419462538054 34-35 21.57282085692776 26.976899283165857 30.604767438054402 20.845512421851986 36-37 21.619525547445257 26.73766816143498 30.51473356027626 21.12807273084351 38-39 21.29285866256424 27.10347042649995 30.6587141173775 20.944956793558312 40-41 21.453585355884268 27.08620508357586 30.553642760202564 20.906566800337306 42-43 21.46992966702731 27.07711011194024 30.319493707351324 21.133466513681128 44-45 21.38532699420641 27.41769909331937 30.04036283591372 21.156611076560505 46-47 21.57822166213872 27.55089849759419 29.618629504762527 21.252250335504566 48-49 21.850962325292134 27.689948774180877 29.164716375896045 21.294372524630944 50-51 21.772720930870072 27.875612858667825 28.91790731805314 21.43375889240896 52-53 21.97401476220091 27.986461163300707 28.82514074825701 21.214383326241368 54-55 21.74456415559654 27.87814625354556 28.827052333651448 21.55023725720645 56-57 21.779176622696475 28.323213642761285 28.74918169618016 21.148428038362084 58-59 22.003494157310726 27.882291087034794 28.688709043893816 21.42550571176066 60-61 21.878232300088378 27.78853392687637 29.101297829858268 21.231935943176982 62-63 21.836580635658407 27.837632156066906 29.233638015122253 21.092149193152434 64-65 21.839244590857213 27.72896708147178 29.004007439201544 21.427780888469464 66-67 21.681675722562275 28.052068672056564 28.81931033354064 21.44694527184053 68-69 21.64880036660011 28.027192235933356 28.91417220385585 21.409835193610686 70-71 21.65968380740401 27.987177179143075 28.920493600864127 21.432645412588787 72-73 21.817943766161502 28.06156099636673 28.549924716048576 21.570570521423193 74-75 21.693545565927785 28.345129526269503 28.358529254060684 21.60279565374203 76-77 21.762442309580702 28.173116264606723 28.594563189420967 21.469878236391608 78-79 21.829379562043798 28.13391951163628 28.411999607214167 21.624701319105757 80-81 21.622062289286767 28.488838335897356 28.45721089326045 21.43188848155543 82-83 21.794154723760762 28.40913414938019 28.43386759484939 21.362843532009663 84-85 21.738618105224912 28.18798001210272 28.397281757259073 21.676120125413295 86-87 21.85178062911198 27.977664397237405 28.56645445320939 21.60410052044123 88-89 21.93381558705116 28.025085103597263 28.5098482864718 21.531251022879776 90-91 21.764852352677483 28.010464937609957 28.59983889708544 21.62484381262712 92-93 21.802420258948725 28.034816181103718 28.384667819950277 21.778095739997283 94-95 21.63785555300972 27.99761055284606 28.378142286668194 21.986391607476026 96-97 21.824081044810317 27.887303197931328 28.308259140453668 21.980356616804684 98-99 21.68892414916734 28.086816661054392 28.119753834521184 22.10450535525708 100-101 21.592532526658296 27.970941365053605 28.53707075307813 21.899455355209973 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 619.0 1 445.0 2 368.0 3 510.0 4 481.0 5 442.5 6 862.5 7 2074.5 8 3398.0 9 4119.5 10 3981.5 11 3584.0 12 3733.0 13 4087.5 14 4404.5 15 4627.5 16 4805.0 17 5034.0 18 5264.0 19 5462.5 20 5758.0 21 6477.0 22 7788.5 23 9473.0 24 11288.5 25 13841.5 26 17134.0 27 20714.0 28 23756.0 29 26572.5 30 31506.0 31 36461.5 32 41001.5 33 47325.0 34 54936.5 35 60706.5 36 66595.5 37 72262.5 38 75688.5 39 76930.5 40 77866.5 41 82818.0 42 87706.0 43 91910.5 44 96688.5 45 100385.0 46 98437.5 47 95580.5 48 97016.0 49 93933.0 50 87876.5 51 81527.0 52 74752.0 53 71431.0 54 68541.0 55 62294.5 56 54523.0 57 47574.0 58 42459.5 59 37631.5 60 31244.0 61 24335.0 62 18582.0 63 14186.0 64 10823.0 65 8366.5 66 6108.0 67 4675.0 68 3330.0 69 2041.0 70 1448.0 71 1059.5 72 775.0 73 569.5 74 453.5 75 351.0 76 210.5 77 129.5 78 75.5 79 46.5 80 34.0 81 23.0 82 15.5 83 12.5 84 9.5 85 6.5 86 2.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01824817518248175 2 0.0 3 0.0 4 0.0 5 0.04222447710385912 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004030146312722987 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.773886288501194E-4 24-25 1.6366076396844622E-4 26-27 0.0012683709207554582 28-29 0.0011865405387712351 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.318974828974503E-4 42-43 0.011292592713822788 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.0915190992111555E-5 52-53 0.0 54-55 1.0228797748027887E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.0457595496055777E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.0457595496055777E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.592190108343425E-4 84-85 4.0915190992111555E-5 86-87 0.0 88-89 0.0 90-91 0.01229501489312952 92-93 0.001800268403652908 94-95 0.0 96-97 0.0 98-99 0.0013502013027396813 100-101 0.019189224575300316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.135099692583445 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2530469531867 34.05654571231367 2 14.706457307371771 13.863806626153814 3 5.250860272504382 7.424994672489596 4 2.5308887545063876 4.771747750179877 5 1.3256005516995657 3.124115707845132 6 0.8342516219563249 2.3593520021766485 7 0.5644669549512127 1.8624344336356093 8 0.39850749293574883 1.502695232621441 9 0.27114518071806065 1.1502409611878408 >10 1.6245695256338792 14.261946213993697 >50 0.1473590622697881 4.818861534621862 >100 0.08789113565199752 7.479080938494828 >500 0.0038250563337768194 1.222723918820387 >1k 8.693309849490938E-4 0.6858973082148515 >5k 8.693309849490939E-5 0.34501794224081783 >10k+ 1.7386619698981878E-4 1.0705390450099295 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14105 0.5771087689437334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12021 0.49184151091617295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8420 0.3445059081535793 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2915 0.11926778174200517 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 2899 0.11861313868613138 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.091519099211155E-5 0.0 0.0 0.0 8 0.0 4.091519099211155E-5 0.0 0.0 0.0 9 0.0 4.091519099211155E-5 0.0 0.0 0.0 10-11 0.0 4.091519099211155E-5 0.0 0.0 0.0 12-13 0.0 4.091519099211155E-5 0.0 0.0 0.0 14-15 0.0 4.091519099211155E-5 0.0 0.0 0.0 16-17 0.0 4.091519099211155E-5 0.0 0.0 0.0 18-19 0.0 4.091519099211155E-5 0.0 0.0 0.0 20-21 0.0 4.091519099211155E-5 0.0 0.0 0.0 22-23 0.0 4.091519099211155E-5 0.0 6.137278648816733E-5 4.091519099211155E-5 24-25 0.0 6.137278648816733E-5 0.0 1.2274557297633465E-4 8.18303819842231E-5 26-27 0.0 1.0228797748027888E-4 0.0 1.84118359464502E-4 8.18303819842231E-5 28-29 0.0 1.2274557297633465E-4 0.0 5.728126738895618E-4 8.18303819842231E-5 30-31 0.0 1.2274557297633465E-4 0.0 0.0015956924486923503 8.18303819842231E-5 32-33 0.0 1.2274557297633465E-4 0.0 0.0029663513469280877 8.18303819842231E-5 34-35 0.0 1.2274557297633465E-4 0.0 0.004582501391116494 8.18303819842231E-5 36-37 0.0 1.2274557297633465E-4 0.0 0.006832836895682629 8.18303819842231E-5 38-39 0.0 1.2274557297633465E-4 0.0 0.008980884422768485 8.18303819842231E-5 40-41 0.0 1.2274557297633465E-4 0.0 0.011660829432751792 8.18303819842231E-5 42-43 0.0 1.2274557297633465E-4 0.0 0.01593646689142745 8.18303819842231E-5 44-45 0.0 1.2274557297633465E-4 0.0 0.022196491113220515 8.18303819842231E-5 46-47 0.0 1.2274557297633465E-4 0.0 0.03498248829825538 1.0228797748027888E-4 48-49 0.0 1.2274557297633465E-4 0.0 0.059408857320545974 1.636607639684462E-4 50-51 0.0 1.2274557297633465E-4 0.0 0.07962096167064908 1.636607639684462E-4 52-53 0.0 1.2274557297633465E-4 0.0 0.10052862426761808 1.636607639684462E-4 54-55 0.0 1.2274557297633465E-4 0.0 0.12761448070439593 1.636607639684462E-4 56-57 0.0 1.2274557297633465E-4 0.0 0.15097705476089163 1.636607639684462E-4 58-59 0.0 1.2274557297633465E-4 0.0 0.17742872573729174 1.636607639684462E-4 60-61 0.0 1.2274557297633465E-4 0.0 0.21701417302215967 1.636607639684462E-4 62-63 0.0 1.2274557297633465E-4 0.0 0.25011456253477793 1.636607639684462E-4 64-65 0.0 1.2274557297633465E-4 0.0 0.2886771300448431 1.636607639684462E-4 66-67 0.0 1.2274557297633465E-4 0.0 0.34857696965729434 1.636607639684462E-4 68-69 0.0 1.2274557297633465E-4 0.0 0.41903292854571045 1.636607639684462E-4 70-71 0.0 1.2274557297633465E-4 0.0 0.4829015416843966 2.0457595496055776E-4 72-73 0.0 1.2274557297633465E-4 0.0 0.5585332722333148 2.0457595496055776E-4 74-75 0.0 1.2274557297633465E-4 0.0 0.6342877483552094 2.0457595496055776E-4 76-77 0.0 1.2274557297633465E-4 0.0 0.7084874472194036 2.0457595496055776E-4 78-79 0.0 1.2274557297633465E-4 0.0 0.7961073287290106 2.0457595496055776E-4 80-81 0.0 1.2274557297633465E-4 0.0 0.92282167523158 2.0457595496055776E-4 82-83 0.0 1.2274557297633465E-4 0.0 1.0428054728159473 2.0457595496055776E-4 84-85 0.0 1.2274557297633465E-4 0.0 1.1752479460574121 2.0457595496055776E-4 86-87 0.0 1.2274557297633465E-4 0.0 1.386431704363196 2.0457595496055776E-4 88-89 0.0 1.2274557297633465E-4 0.0 1.661259042257209 2.0457595496055776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7030 0.0 54.07588 1 GTATCAA 12275 0.0 39.989662 1 ATCAACG 16120 0.0 30.094978 3 TATCAAC 16220 0.0 30.026613 2 TCAACGC 16245 0.0 29.834162 4 CAACGCA 16505 0.0 29.364187 5 AACGCAG 16920 0.0 28.661491 6 ACGCAGA 19545 0.0 24.643496 7 CGCAGAG 19535 0.0 24.583164 8 GTACATG 11795 0.0 24.373976 1 TACATGG 12015 0.0 23.490704 2 GAGTACT 12410 0.0 22.755173 12-13 GCAGAGT 20740 0.0 22.742622 9 ACATGGG 11805 0.0 22.620579 3 GTACTTT 13025 0.0 21.642948 14-15 AGTACTT 12700 0.0 21.58103 12-13 AGAGTAC 19450 0.0 20.245802 10-11 CAGAGTA 20460 0.0 20.140207 10-11 TGGTATC 3305 0.0 19.83997 2 CATGGGG 5020 0.0 19.498306 4 >>END_MODULE