FastQCFastQC Report
Thu 26 May 2016
SRR937734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303104
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120390.5227293252931695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91350.3966386233535264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62930.2732399405324293No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32110.13942053854276662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.053.252981
GTATCAA108200.043.2434651
TATCAAC138050.033.7450372
ATCAACG138700.033.5511633
TCAACGC140450.033.1035964
CAACGCA141550.032.779895
AACGCAG144350.032.190296
ACGCAGA164700.028.0103787
CGCAGAG164500.027.8428678
GTACATG108300.025.9923731
GCAGAGT171450.025.965439
TACATGG109150.025.041732
ACATGGG110550.023.6915873
GAGTACT98400.023.6778812-13
AGTACTT103950.022.96203812-13
GTGTAGC12950.022.0309961
AGAGTAC160200.021.86793710-11
GTACTTT106700.021.67997214-15
CAGAGTA169550.021.4884910-11
TGGTATC26400.021.2470342
Produced by FastQC (version 0.11.5)