FastQCFastQC Report
Thu 26 May 2016
SRR937734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303104
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136080.5908547768576669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113970.4948539015172567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80660.3502230033902073No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA28580.1240933974323348No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27730.12040272606013451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64800.049.2144361
GTATCAA112100.040.3199421
ATCAACG148150.030.4038813
TATCAAC150000.030.1239282
TCAACGC150700.029.9209464
CAACGCA152350.029.6280785
AACGCAG156900.028.8194356
GTACATG112700.025.9355951
TACATGG116050.024.852262
ACGCAGA181650.024.7380187
CGCAGAG181200.024.6683798
ACATGGG115200.024.169493
GCAGAGT190600.023.1028759
GAGTACT112700.022.2545712-13
AGTACTT117000.021.90356412-13
TGGTATC33000.021.1653652
AGAGTAC179650.021.06045310-11
CATGGGG46550.021.026644
GTACTTT119000.021.01468714-15
CGGGTCG1456.150718E-419.6541716
Produced by FastQC (version 0.11.5)