##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937734_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2303104 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.185412816789864 33.0 31.0 34.0 31.0 34.0 2 32.400717900711385 34.0 31.0 34.0 31.0 34.0 3 32.486601560329014 34.0 31.0 34.0 31.0 34.0 4 36.122536368309895 37.0 35.0 37.0 35.0 37.0 5 36.03239106874896 37.0 35.0 37.0 35.0 37.0 6 36.068891808620016 37.0 35.0 37.0 35.0 37.0 7 36.01283745762241 37.0 35.0 37.0 35.0 37.0 8 35.99278234938587 37.0 35.0 37.0 35.0 37.0 9 37.6591678013672 39.0 37.0 39.0 35.0 39.0 10-11 37.59138775322347 39.0 37.0 39.0 35.0 39.0 12-13 37.491015603290165 39.0 37.0 39.0 35.0 39.0 14-15 38.68841702328683 40.0 38.0 41.0 34.5 41.0 16-17 38.65047192831935 40.0 38.0 41.0 34.5 41.0 18-19 38.65263183946534 40.0 38.0 41.0 34.0 41.0 20-21 38.55243336818485 40.0 38.0 41.0 34.0 41.0 22-23 38.393140083991 40.0 38.0 41.0 34.0 41.0 24-25 38.468021418051464 40.0 38.0 41.0 34.0 41.0 26-27 38.34948486911577 40.0 38.0 41.0 34.0 41.0 28-29 38.20473869178292 40.0 38.0 41.0 34.0 41.0 30-31 38.245188450022226 40.0 38.0 41.0 34.0 41.0 32-33 38.16962759823265 40.0 37.0 41.0 34.0 41.0 34-35 38.03689520751126 40.0 37.0 41.0 33.5 41.0 36-37 37.87430875896182 40.0 37.0 41.0 33.0 41.0 38-39 37.73827538834547 40.0 37.0 41.0 33.0 41.0 40-41 37.55042347197521 40.0 37.0 41.0 33.0 41.0 42-43 37.32926780553549 40.0 37.0 41.0 32.5 41.0 44-45 37.000894227963656 40.0 36.0 41.0 31.5 41.0 46-47 36.620955458372705 39.5 36.0 41.0 30.5 41.0 48-49 36.271751731576174 39.0 36.0 41.0 30.0 41.0 50-51 36.0188966716223 39.0 35.5 41.0 29.5 41.0 52-53 35.77585488974879 39.0 35.0 40.0 28.5 41.0 54-55 35.548585300533546 39.0 35.0 40.0 28.0 41.0 56-57 35.29775641916301 38.0 35.0 40.0 27.5 41.0 58-59 35.03467212075529 38.0 34.0 40.0 27.0 41.0 60-61 34.69163311774023 38.0 34.0 40.0 26.0 41.0 62-63 34.34487956253821 37.5 34.0 40.0 26.0 41.0 64-65 33.94763718876786 37.0 33.5 39.5 25.5 41.0 66-67 33.51594956198244 36.0 33.0 39.0 24.5 40.5 68-69 33.072521909562056 36.0 32.0 39.0 22.5 40.0 70-71 32.652467061843495 35.0 32.0 38.5 22.0 40.0 72-73 32.15241039918301 35.0 31.0 38.0 20.5 40.0 74-75 31.625992573500806 35.0 31.0 37.0 19.5 39.0 76-77 30.3865194537459 33.5 29.5 35.5 18.5 38.5 78-79 31.007662050867005 34.0 31.0 36.0 18.5 38.5 80-81 30.80260118518313 34.0 31.0 36.0 18.0 37.5 82-83 30.426165948215974 34.0 30.5 35.0 16.0 37.0 84-85 30.02467344071305 34.0 30.0 35.0 10.0 36.5 86-87 29.56875568797588 34.0 29.5 35.0 7.0 36.0 88-89 29.183462839715446 34.0 29.0 35.0 2.0 36.0 90-91 28.635687532998944 34.0 29.0 35.0 2.0 35.0 92-93 28.124538666078475 33.0 28.0 35.0 2.0 35.0 94-95 27.620993884774634 33.0 27.0 35.0 2.0 35.0 96-97 27.036663997804702 33.0 26.0 35.0 2.0 35.0 98-99 26.11493879564275 32.5 24.5 34.0 2.0 35.0 100-101 23.427232769340854 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26870589746508955 1101 2 0.2740995269292341 1101 3 0.13680880054810984 1101 4 0.11509275271351527 1101 5 0.11272874830706314 1101 6 0.16568103853170868 1101 7 0.23152491058856128 1101 8 0.21590916480024447 1101 9 0.2545721862323447 1101 10-11 0.24516974163924488 1101 12-13 0.2030169299170197 1101 14-15 0.27127896764569215 1101 16-17 0.26313435913660044 1101 18-19 0.3430901053753672 1101 20-21 0.3581339908996526 1101 22-23 0.2168386453788429 1101 24-25 0.2146443026905871 1101 26-27 0.18274516951726838 1101 28-29 0.23083531053306672 1101 30-31 0.2957891156462651 1101 32-33 0.34388242291025506 1101 34-35 0.342292534127111 1101 36-37 0.2806165069734874 1101 38-39 0.2551517142330866 1101 40-41 0.2724263954445263 1101 42-43 0.3791583484378904 1101 44-45 0.5332038197850011 1101 46-47 0.5249982120801278 1101 48-49 0.5228367410072394 1101 50-51 0.569981095656118 1101 52-53 0.4408178236499225 1101 54-55 0.5745254520549281 1101 56-57 0.5510532123214489 1101 58-59 0.512712748931321 1101 60-61 0.4866229542313789 1101 62-63 0.4780760958757142 1101 64-65 0.5505527403300334 1101 66-67 0.35738044585814777 1101 68-69 0.3906210687997387 1101 70-71 0.5680658626417028 1101 72-73 0.5003319980016556 1101 74-75 0.4331740293978328 1101 76-77 0.4254162267079913 1101 78-79 0.46594565998232795 1101 80-81 0.5499526184178158 1101 82-83 0.5366430076811888 1101 84-85 0.5885745832483522 1101 86-87 0.2859328111658215 1101 88-89 0.6039822641457739 1101 90-91 0.553903011329286 1101 92-93 0.6401591223047163 1101 94-95 0.65868727852002 1101 96-97 0.8034602281924528 1101 98-99 0.987023414749423 1101 100-101 1.3776503284720292 1102 1 0.2060838648995187 1102 2 0.23039456670117886 1102 3 0.16294872827832307 1102 4 0.10175288152689177 1102 5 0.10233709555541992 1102 6 0.08795450338780597 1102 7 0.15302855794499948 1102 8 0.16090697079092564 1102 9 0.14453599244095017 1102 10-11 0.11448144115713887 1102 12-13 0.23137634103903082 1102 14-15 0.3810377444192383 1102 16-17 0.248689679093836 1102 18-19 0.21955692576993613 1102 20-21 0.23123720357720146 1102 22-23 0.27325914125439965 1102 24-25 0.12237001324038488 1102 26-27 0.1594867564486364 1102 28-29 0.17472020897546514 1102 30-31 0.11101347886818758 1102 32-33 0.12205041039999287 1102 34-35 0.15306202767907706 1102 36-37 0.2039260154270437 1102 38-39 0.23510450622038803 1102 40-41 0.2644223843831952 1102 42-43 0.3247561938912895 1102 44-45 0.4938746565418697 1102 46-47 0.5828658706157057 1102 48-49 0.4944141600865848 1102 50-51 0.3059170541436984 1102 52-53 0.2595162312997985 1102 54-55 0.3442787511676073 1102 56-57 0.07337998129668932 1102 58-59 0.17597487267345002 1102 60-61 0.2531379544817014 1102 62-63 0.1575439970775605 1102 64-65 0.20018785743711476 1102 66-67 0.16484964874069163 1102 68-69 0.2317032307676925 1102 70-71 0.11988954086152148 1102 72-73 0.2346937179871631 1102 74-75 0.2998443772881956 1102 76-77 0.2637016894083608 1102 78-79 0.4141065206229797 1102 80-81 0.34655116772118433 1102 82-83 0.46870896931989137 1102 84-85 0.3941298443060113 1102 86-87 0.4505142854811872 1102 88-89 0.48822765746243846 1102 90-91 0.5735457264820845 1102 92-93 0.6255981155931067 1102 94-95 0.8048047655105748 1102 96-97 0.7959338927181179 1102 98-99 0.5780300380070997 1102 100-101 1.0345353947782279 1103 1 0.20009006210212021 1103 2 0.19833818023577265 1103 3 0.1950807044589098 1103 4 0.09390857325934121 1103 5 0.048882760975722306 1103 6 0.07773027313345438 1103 7 0.15597540279566857 1103 8 0.10578071865595007 1103 9 0.17341155215105886 1103 10-11 0.08985116718770314 1103 12-13 0.09891185666474911 1103 14-15 0.12055428760346132 1103 16-17 0.16614484458337841 1103 18-19 0.090653813320138 1103 20-21 0.0326053197199343 1103 22-23 0.12590795489978746 1103 24-25 0.17717544185138223 1103 26-27 0.06249664081367712 1103 28-29 0.017974692426513172 1103 30-31 0.16277526740163495 1103 32-33 0.08112810047061458 1103 34-35 0.10132173316770121 1103 36-37 0.16075779657462874 1103 38-39 0.042310341053976686 1103 40-41 0.09955905174227553 1103 42-43 0.15586958423427433 1103 44-45 0.1472826852082605 1103 46-47 0.22039861654193516 1103 48-49 0.17956308913610997 1103 50-51 0.2831688775291923 1103 52-53 0.2074007290609572 1103 54-55 0.16757091407210112 1103 56-57 0.03640406037160915 1103 58-59 0.17833604148663085 1103 60-61 0.1453496172246247 1103 62-63 0.10873338105023578 1103 64-65 0.09434855193926239 1103 66-67 0.1899157176138786 1103 68-69 0.12104839879612683 1103 70-71 0.2731688939058827 1103 72-73 0.2072663360456133 1103 74-75 0.27812502862919075 1103 76-77 0.20087523993229794 1103 78-79 0.3201809620669174 1103 80-81 0.2881208949057843 1103 82-83 0.3262879923083979 1103 84-85 0.14081284065924393 1103 86-87 0.3757818788431422 1103 88-89 0.3217225565992159 1103 90-91 0.38986142029180826 1103 92-93 0.4214454416790616 1103 94-95 0.4242086742489768 1103 96-97 0.6437387616190087 1103 98-99 0.6141164814821494 1103 100-101 0.951275198821989 1104 1 0.16075187320736717 1104 2 0.08293013954326511 1104 3 -0.020084670847026587 1104 4 0.011790679687294414 1104 5 -0.01894339775572007 1104 6 0.08204354192128704 1104 7 0.05171013746949171 1104 8 0.06035096372150406 1104 9 0.14351686115362128 1104 10-11 0.15798544673012316 1104 12-13 0.17116051741324156 1104 14-15 0.13516780621213087 1104 16-17 0.1892827102998993 1104 18-19 0.04106833617023398 1104 20-21 0.06785884786167884 1104 22-23 0.11785258527312692 1104 24-25 0.10227764640472259 1104 26-27 0.16710186023231444 1104 28-29 0.08709749395811173 1104 30-31 0.1542452860846666 1104 32-33 0.0989006497023297 1104 34-35 0.1259950617232235 1104 36-37 0.16695606044942934 1104 38-39 0.11488571550351168 1104 40-41 0.13298927938686234 1104 42-43 0.14693971492538793 1104 44-45 0.2208867958145504 1104 46-47 0.04330422607321793 1104 48-49 -0.09608340818876115 1104 50-51 -0.03431218589397389 1104 52-53 -0.002714634187775289 1104 54-55 -0.005675452426444849 1104 56-57 -0.13495625462574878 1104 58-59 -0.1232485234848113 1104 60-61 -0.06870370605727771 1104 62-63 0.017367003643862233 1104 64-65 0.011803374033476643 1104 66-67 0.002471073718439243 1104 68-69 0.013700728090860537 1104 70-71 0.030488506249760405 1104 72-73 -0.1108459700812574 1104 74-75 -0.18068763504458474 1104 76-77 -0.21987094627155201 1104 78-79 -0.04087303224929073 1104 80-81 0.02861316702456307 1104 82-83 0.13286650895635788 1104 84-85 -0.021931148766476838 1104 86-87 0.06291995133512884 1104 88-89 0.04552799158269849 1104 90-91 0.03299913670177901 1104 92-93 0.15971579300580174 1104 94-95 -0.028694337911900902 1104 96-97 0.21127891381026487 1104 98-99 0.1875208593753186 1104 100-101 0.3354164918279068 1105 1 0.15810279356791312 1105 2 0.12854522890288678 1105 3 0.11213870022148598 1105 4 0.06718737462905011 1105 5 -0.012549836681500892 1105 6 0.02276265236918107 1105 7 0.02483639231053303 1105 8 0.05556410416649271 1105 9 0.1490397398173542 1105 10-11 0.13752209555810424 1105 12-13 0.182379232918926 1105 14-15 0.19359695038055946 1105 16-17 0.24026034482958636 1105 18-19 0.18378639708601696 1105 20-21 0.17646732567401102 1105 22-23 0.22282557476055587 1105 24-25 0.22519767702379312 1105 26-27 0.11631801461032154 1105 28-29 0.11099433850237972 1105 30-31 0.174811107612868 1105 32-33 0.30194387199314576 1105 34-35 0.15913001918014658 1105 36-37 0.0980358034276847 1105 38-39 -0.011276334105609465 1105 40-41 0.14740856283502524 1105 42-43 0.17364534136854815 1105 44-45 0.09499175828346296 1105 46-47 -0.06455708082570766 1105 48-49 -0.016059530702065672 1105 50-51 0.11600824964080658 1105 52-53 0.07194291072349301 1105 54-55 -0.0061528759134859 1105 56-57 0.061492674106645495 1105 58-59 -0.05314217735046611 1105 60-61 -0.11080534570922396 1105 62-63 -0.01874948347875005 1105 64-65 -0.012294202994979742 1105 66-67 -0.16433635818085435 1105 68-69 -0.2676752951924257 1105 70-71 -0.02403729587009451 1105 72-73 -0.12347444202731594 1105 74-75 -0.2203634415620357 1105 76-77 -0.12761017247097328 1105 78-79 -0.02332177497800103 1105 80-81 -0.11573242613993173 1105 82-83 0.08578485207592834 1105 84-85 0.07277734095513821 1105 86-87 0.2047904750954146 1105 88-89 0.07575443213284672 1105 90-91 0.27115960233381387 1105 92-93 0.37212505834595433 1105 94-95 0.3945717773927093 1105 96-97 0.3413144270478803 1105 98-99 0.5848990069721864 1105 100-101 0.7034401437535749 1106 1 -0.07521233520436965 1106 2 3.408025442439566E-4 1106 3 0.02470336660226735 1106 4 -0.026679078622812824 1106 5 -0.03141045708772339 1106 6 -0.015457367347359252 1106 7 -0.018360120060528118 1106 8 -0.0801402537621172 1106 9 -0.08776423442514414 1106 10-11 -0.12960072787807775 1106 12-13 -0.2174511474955736 1106 14-15 -0.023338418214017054 1106 16-17 0.01446730592352452 1106 18-19 -0.023123645879351784 1106 20-21 -0.07438783249494918 1106 22-23 -0.13225112987500154 1106 24-25 -0.03712439946055923 1106 26-27 0.01292371120415936 1106 28-29 0.04664186691822181 1106 30-31 0.040644144416063455 1106 32-33 0.08759428967702831 1106 34-35 -0.19837997051842393 1106 36-37 -0.2113153017282059 1106 38-39 -0.06535322165571955 1106 40-41 0.10668074535334426 1106 42-43 -0.17668536034125282 1106 44-45 -0.046656236760028946 1106 46-47 0.039316015570271645 1106 48-49 0.06823931780513703 1106 50-51 0.17664355840798152 1106 52-53 0.03960418075882899 1106 54-55 0.031085444383379013 1106 56-57 0.10510172181692212 1106 58-59 0.056408004890656116 1106 60-61 0.17425070826317324 1106 62-63 0.12068203022398194 1106 64-65 0.01711224576439463 1106 66-67 -0.16139755982961645 1106 68-69 -0.1243904103221567 1106 70-71 0.1326961541636038 1106 72-73 0.10196906061712951 1106 74-75 -0.016112611386258635 1106 76-77 -0.1662468550525098 1106 78-79 -0.1353771481258832 1106 80-81 -0.16087148248326244 1106 82-83 -0.313612789846875 1106 84-85 -0.16263196405207125 1106 86-87 -0.04149811889985955 1106 88-89 -0.3424230175862881 1106 90-91 -0.3185128502550363 1106 92-93 -0.16591682604769886 1106 94-95 0.10954830915011016 1106 96-97 0.18975168061829706 1106 98-99 0.23302308995543797 1106 100-101 0.2615528825580924 1107 1 0.21925950848267917 1107 2 0.2829182359960072 1107 3 0.2432660076551656 1107 4 0.12514986072324774 1107 5 0.08939418684448697 1107 6 0.12578737758431657 1107 7 0.1669245713707923 1107 8 0.15615411306001192 1107 9 0.24617305269870116 1107 10-11 0.2468513787778619 1107 12-13 0.260750015083552 1107 14-15 0.28131955943458564 1107 16-17 0.24466906543372602 1107 18-19 0.24772496984707004 1107 20-21 0.29545382116372565 1107 22-23 0.29737253221732374 1107 24-25 0.25240943567792584 1107 26-27 0.42929534389044477 1107 28-29 0.4491098736472665 1107 30-31 0.31707645977442667 1107 32-33 0.31437015961628845 1107 34-35 0.3860050947130631 1107 36-37 0.31778165647703815 1107 38-39 0.4229112571149116 1107 40-41 0.3926260511448447 1107 42-43 0.47869922926065556 1107 44-45 0.41683703898701907 1107 46-47 0.34667718121385604 1107 48-49 0.39067219498097927 1107 50-51 0.34956275883955357 1107 52-53 0.2567724929761255 1107 54-55 0.2900103619462442 1107 56-57 0.1977648962772136 1107 58-59 0.16241450409740565 1107 60-61 0.29953848011420803 1107 62-63 0.2900160530605902 1107 64-65 0.4040303576745998 1107 66-67 0.46745168794146963 1107 68-69 0.3794202124972159 1107 70-71 0.3660367496262822 1107 72-73 0.4135290936877496 1107 74-75 0.38592461540604717 1107 76-77 0.23722611865379406 1107 78-79 0.2640669398623956 1107 80-81 0.4365103203762608 1107 82-83 0.39971257908525715 1107 84-85 0.4147181727313338 1107 86-87 0.5885933520085302 1107 88-89 0.6790211725506268 1107 90-91 0.7993223010047465 1107 92-93 0.7184529966925055 1107 94-95 0.8284843513374973 1107 96-97 0.9949974119227001 1107 98-99 0.9620251616573974 1107 100-101 1.2069610621098512 1108 1 0.06150239709128158 1108 2 0.06594471114300404 1108 3 0.0323672081044748 1108 4 0.0083090567422488 1108 5 -0.005528220078332424 1108 6 -0.053933626199643925 1108 7 -0.003289790094342493 1108 8 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-0.06520688020011534 2316 60-61 -0.005045714100674559 2316 62-63 -0.10216176202205673 2316 64-65 -0.2098965534824231 2316 66-67 -0.35344836371141497 2316 68-69 -0.10280796415568005 2316 70-71 0.02163463185768677 2316 72-73 0.003030643274520628 2316 74-75 -0.06171872137080925 2316 76-77 0.051985932703381366 2316 78-79 -0.12782355600537088 2316 80-81 0.020698078640723594 2316 82-83 0.015350492308396468 2316 84-85 -0.03808399669015472 2316 86-87 -0.1267830684460165 2316 88-89 -0.003536291292348892 2316 90-91 0.21194820493036204 2316 92-93 0.11502130764291607 2316 94-95 0.06506428418873256 2316 96-97 0.23979015469129905 2316 98-99 0.4794967525664866 2316 100-101 1.136403210870185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 12.0 5 51.0 6 76.0 7 226.0 8 539.0 9 1099.0 10 1766.0 11 2984.0 12 4763.0 13 6892.0 14 9002.0 15 12795.0 16 17908.0 17 25818.0 18 23122.0 19 13581.0 20 11271.0 21 11282.0 22 12217.0 23 12970.0 24 15202.0 25 18422.0 26 22230.0 27 27410.0 28 34533.0 29 43164.0 30 54355.0 31 69206.0 32 88312.0 33 115889.0 34 158857.0 35 226473.0 36 343165.0 37 505474.0 38 381196.0 39 30829.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.472689592035444 20.259558534214772 15.474971358489222 21.792780515260564 2 15.289626521424998 21.02024051019841 40.60064156894348 23.08949139943311 3 19.208164286111266 25.36746060968154 30.616680792530428 24.807694311676766 4 12.76384004168295 17.525726195128303 37.46545959793322 32.24497416525553 5 13.120216388717942 38.47601088023877 34.209560555964046 14.19421217507925 6 31.360329364197188 37.10058251820153 17.283804812982826 14.255283304618462 7 28.013889081865166 33.84675637748013 20.556388248207636 17.582966292447065 8 26.514651531151003 35.945228700050016 19.342331045406546 18.19778872339243 9 26.51347920024454 17.032752320346802 20.712308258767298 35.74146022064136 10-11 24.680273674315405 27.657757307805447 27.0699415245305 20.592027493348645 12-13 25.61009896841413 25.002415319967476 28.205249005919438 21.182236705698962 14-15 22.45206903379092 26.085860647196128 26.88202530150614 24.58004501750681 16-17 21.198565066970488 29.76969342244206 27.44248631412216 21.58925519646529 18-19 21.092555959261936 27.67871099177458 30.56190688740066 20.666826161562827 20-21 21.915771063747012 26.738935801422777 30.882322292002446 20.462970842827765 22-23 21.775504205019278 26.819877372962566 30.670132748262002 20.734485673756158 24-25 21.54385993858723 26.913135490190633 30.925959053520813 20.617045517701328 26-27 21.38610525365123 27.285019040694614 30.659806039138225 20.669069666515927 28-29 20.983531817752553 27.19261509871031 31.016541996524218 20.80731108701292 30-31 21.140143910131716 26.961895771966876 31.037243650308454 20.860716667592953 32-33 21.049006037069972 27.113864593175123 30.823510358194856 21.01361901156005 34-35 21.59346690379592 26.975985452676042 30.723145806702608 20.707401836825433 36-37 21.646938219029625 26.755109625965652 30.552180882843327 21.045771272161396 38-39 21.337608725621198 27.11405593107558 30.723665142997326 20.824670200305892 40-41 21.427713884079523 27.12115801071388 30.62256644612938 20.828561659077216 42-43 21.486669993142943 27.05953827988172 30.35513825504172 21.098653471933616 44-45 21.413436567969388 27.3727879300231 30.102387952253977 21.111387549753527 46-47 21.622275850330684 27.529021702884453 29.649377535708332 21.19932491107653 48-49 21.869160055299282 27.691584921914075 29.23068172344801 21.20857329933863 50-51 21.8178380133941 27.86040057244484 28.969251931306616 21.352509482854444 52-53 22.002072420974567 28.021298217817826 28.803742428423213 21.172886932784394 54-55 21.751840125326517 27.92392353970989 28.841988898460514 21.482247436503084 56-57 21.820942519313068 28.3442475893403 28.789516235480466 21.04529365586617 58-59 22.02757669649308 27.88158936808759 28.704174887456237 21.386659047963096 60-61 21.878386733729783 27.75489079086311 29.147598197910295 21.219124277496803 62-63 21.798429423942643 27.850566018729506 29.258448598065918 21.092555959261936 64-65 21.836660437392318 27.708800818373813 29.068509281387207 21.38602946284666 66-67 21.697391867670763 28.07971329127994 28.818650829489243 21.40424401156005 68-69 21.62272882399477 28.040442846213498 28.922731168658615 21.414097161133117 70-71 21.67857415005448 28.070232188870364 28.88124654406543 21.369947117009723 72-73 21.84788442033013 28.06577557939199 28.56212745928972 21.52421254098816 74-75 21.72702578780637 28.345810697215583 28.399824758239316 21.527338756738732 76-77 21.753468362696605 28.21935092813872 28.61090945089757 21.416271258267102 78-79 21.81992215722781 28.168397953370754 28.418451793753125 21.593228095648307 80-81 21.656969959856305 28.52533854890894 28.435893945620723 21.381797545614035 82-83 21.792424647684285 28.43929001913985 28.426024989492188 21.342260343683677 84-85 21.758456996743075 28.253961086243585 28.296838208444036 21.6907437085693 86-87 21.911168579447562 28.010654316956597 28.54413000889235 21.534047094703496 88-89 21.95037653531929 28.01773172205858 28.4824306674818 21.549461075140332 90-91 21.77490374953427 28.05749928566652 28.57690006192338 21.590696902875834 92-93 21.787241692015403 28.082897369803096 28.447914832587617 21.681946105593887 94-95 21.65468862891124 28.029997776913245 28.35751229644862 21.957801297726895 96-97 21.861301096259655 27.931956177402324 28.29001643000056 21.916726296337465 98-99 21.697829131576647 28.135686169449443 28.115300287942436 22.05118441103148 100-101 21.582811295558425 28.00714386528711 28.565429337618202 21.844615501536257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 574.0 1 426.0 2 364.0 3 478.0 4 442.5 5 427.5 6 842.5 7 2002.5 8 3317.0 9 3997.0 10 3847.5 11 3505.0 12 3608.0 13 3896.0 14 4175.0 15 4464.5 16 4669.5 17 4797.0 18 4917.5 19 5110.0 20 5507.0 21 6230.0 22 7391.0 23 8944.0 24 10762.5 25 13087.5 26 16222.5 27 19686.5 28 22321.5 29 25143.0 30 29795.0 31 34294.5 32 38757.5 33 44472.0 34 51775.0 35 57658.0 36 63256.5 37 68300.0 38 71005.5 39 72477.5 40 73586.0 41 78123.5 42 82793.0 43 86576.5 44 91201.5 45 94962.0 46 92826.5 47 89105.5 48 90638.5 49 88544.0 50 82656.0 51 76928.0 52 70708.0 53 67105.0 54 64270.5 55 58342.0 56 51246.5 57 44808.0 58 39492.5 59 34876.5 60 29132.5 61 22801.5 62 17416.0 63 13570.5 64 10458.0 65 7849.0 66 5736.5 67 4442.5 68 3201.0 69 2005.0 70 1382.5 71 1012.0 72 770.5 73 552.5 74 430.5 75 351.0 76 213.5 77 118.5 78 77.5 79 47.5 80 27.0 81 18.0 82 14.5 83 11.0 84 4.5 85 2.5 86 2.0 87 1.0 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02179667092758295 2 0.0 3 0.0 4 1.736786528094259E-4 5 0.025226824320569108 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.512949480353471E-5 12-13 0.004189997499027399 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.986522536541988E-4 24-25 0.0 26-27 4.5590646362474293E-4 28-29 0.0011506210748624466 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.512949480353471E-5 40-41 7.164244428388818E-4 42-43 0.015935016395264824 44-45 4.3419663202356475E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.1709831601178238E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.683932640471295E-5 70-71 2.1709831601178238E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0854915800589117E-4 82-83 0.0024315011393319626 84-85 4.7761629522592117E-4 86-87 0.0 88-89 0.0 90-91 0.01120227310620797 92-93 0.002757148613349636 94-95 0.0 96-97 0.0 98-99 0.0017150766964930807 100-101 0.018627035513810924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2303104.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.74489792951779 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68680424800976 34.70424049644066 2 14.460237540314939 13.808051307978362 3 5.208578419258037 7.460491349560922 4 2.458723389106241 4.695659889991821 5 1.3783015400772174 3.2903433173541954 6 0.8051277130697708 2.3064444288445376 7 0.5477971072734197 1.830816188099817 8 0.3678945290451147 1.4052069390469615 9 0.28217082251848014 1.2124995407849621 >10 1.5718740602131083 14.048624100880424 >50 0.14559397509518235 4.847014737952527 >100 0.08216300634787273 7.1461813867186255 >500 0.003823332426389545 1.2454323772224176 >1k 6.372220710649075E-4 0.5616289366067967 >5k 9.103172443784393E-5 0.3505725895891056 >10k+ 1.8206344887568786E-4 1.086792412927794 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13608 0.5908547768576669 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11397 0.4948539015172567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8066 0.3502230033902073 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 2858 0.1240933974323348 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2773 0.12040272606013451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.3419663202356475E-5 0.0 12-13 0.0 0.0 0.0 4.3419663202356475E-5 0.0 14-15 0.0 0.0 0.0 4.3419663202356475E-5 0.0 16-17 0.0 0.0 0.0 1.3025898960706943E-4 0.0 18-19 0.0 0.0 0.0 1.736786528094259E-4 0.0 20-21 0.0 0.0 0.0 1.736786528094259E-4 2.1709831601178238E-5 22-23 0.0 0.0 0.0 1.9538848441060414E-4 6.512949480353471E-5 24-25 0.0 0.0 0.0 3.039376424164953E-4 8.683932640471295E-5 26-27 0.0 0.0 0.0 4.993261268270994E-4 8.683932640471295E-5 28-29 0.0 0.0 0.0 0.0010203620852553771 8.683932640471295E-5 30-31 0.0 0.0 0.0 0.0023880814761296056 8.683932640471295E-5 32-33 2.1709831601178238E-5 0.0 0.0 0.0043202564886344694 8.683932640471295E-5 34-35 4.3419663202356475E-5 0.0 0.0 0.006122172511532262 8.683932640471295E-5 36-37 4.3419663202356475E-5 0.0 0.0 0.008835901461679542 8.683932640471295E-5 38-39 4.3419663202356475E-5 0.0 0.0 0.011267402601011504 8.683932640471295E-5 40-41 4.3419663202356475E-5 0.0 0.0 0.013981131551158784 8.683932640471295E-5 42-43 4.3419663202356475E-5 0.0 0.0 0.018735584671816817 8.683932640471295E-5 44-45 4.3419663202356475E-5 0.0 0.0 0.025574181626187964 8.683932640471295E-5 46-47 4.3419663202356475E-5 0.0 0.0 0.04120526037903629 8.683932640471295E-5 48-49 4.3419663202356475E-5 0.0 0.0 0.07094772967265048 8.683932640471295E-5 50-51 4.3419663202356475E-5 0.0 0.0 0.09580548685599956 8.683932640471295E-5 52-53 4.3419663202356475E-5 0.0 0.0 0.12129282915578281 1.3025898960706943E-4 54-55 4.3419663202356475E-5 0.0 0.0 0.15288063413549713 1.736786528094259E-4 56-57 4.3419663202356475E-5 0.0 0.0 0.18086460706941587 1.736786528094259E-4 58-59 4.3419663202356475E-5 0.0 0.0 0.21266951036514198 1.736786528094259E-4 60-61 4.3419663202356475E-5 0.0 0.0 0.25891145167565166 1.736786528094259E-4 62-63 4.3419663202356475E-5 0.0 0.0 0.29727272411493355 1.736786528094259E-4 64-65 4.3419663202356475E-5 0.0 0.0 0.34125684293892067 1.736786528094259E-4 66-67 4.3419663202356475E-5 0.0 0.0 0.41140130884232756 1.736786528094259E-4 68-69 4.3419663202356475E-5 0.0 0.0 0.48790675540487966 1.736786528094259E-4 70-71 4.3419663202356475E-5 0.0 0.0 0.5589630342355361 1.736786528094259E-4 72-73 4.3419663202356475E-5 0.0 0.0 0.64397873478575 1.736786528094259E-4 74-75 4.3419663202356475E-5 0.0 0.0 0.725216924637359 1.736786528094259E-4 76-77 4.3419663202356475E-5 0.0 0.0 0.8057169802145279 1.736786528094259E-4 78-79 4.3419663202356475E-5 0.0 0.0 0.8996120018896238 1.736786528094259E-4 80-81 4.3419663202356475E-5 0.0 0.0 1.0327366892680487 1.736786528094259E-4 82-83 4.3419663202356475E-5 0.0 0.0 1.1581543864280555 1.736786528094259E-4 84-85 4.3419663202356475E-5 0.0 0.0 1.3031109320291225 1.736786528094259E-4 86-87 4.3419663202356475E-5 0.0 0.0 1.5288931806813761 1.736786528094259E-4 88-89 4.3419663202356475E-5 0.0 0.0 1.8139866892680487 1.736786528094259E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6480 0.0 49.214436 1 GTATCAA 11210 0.0 40.319942 1 ATCAACG 14815 0.0 30.403881 3 TATCAAC 15000 0.0 30.123928 2 TCAACGC 15070 0.0 29.920946 4 CAACGCA 15235 0.0 29.628078 5 AACGCAG 15690 0.0 28.819435 6 GTACATG 11270 0.0 25.935595 1 TACATGG 11605 0.0 24.85226 2 ACGCAGA 18165 0.0 24.738018 7 CGCAGAG 18120 0.0 24.668379 8 ACATGGG 11520 0.0 24.16949 3 GCAGAGT 19060 0.0 23.102875 9 GAGTACT 11270 0.0 22.25457 12-13 AGTACTT 11700 0.0 21.903564 12-13 TGGTATC 3300 0.0 21.165365 2 AGAGTAC 17965 0.0 21.060453 10-11 CATGGGG 4655 0.0 21.02664 4 GTACTTT 11900 0.0 21.014687 14-15 CGGGTCG 145 6.150718E-4 19.654171 6 >>END_MODULE