FastQCFastQC Report
Thu 26 May 2016
SRR937733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157850
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112850.5229742567833724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88960.41226220543596637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.27726672382232315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31940.14801770280603377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54700.053.8910831
GTATCAA95200.041.0532381
TATCAAC122050.031.3898072
ATCAACG121850.031.3589343
TCAACGC123200.031.0531654
CAACGCA125600.030.4597935
AACGCAG129400.029.7004766
GTACATG102900.026.8918171
TACATGG103350.026.4453162
ACGCAGA148400.025.6737697
CGCAGAG147600.025.6519938
ACATGGG103350.025.108053
CGACACG1151.2777164E-424.7955744
CATGGGG40950.024.1396054
GCAGAGT154650.023.804589
GAGTACT88500.023.24038512-13
TGGTATC24550.021.491312
CAGAGTA152250.021.04302810-11
GTACTTT98100.020.9171914-15
AGTACTT94000.020.69306212-13
Produced by FastQC (version 0.11.5)