##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937733_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2157850 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68457168014459 33.0 31.0 34.0 30.0 34.0 2 31.822017749148458 33.0 31.0 34.0 30.0 34.0 3 31.838049447366593 33.0 31.0 34.0 30.0 34.0 4 35.44913038441041 37.0 35.0 37.0 33.0 37.0 5 35.43995087703038 37.0 35.0 37.0 33.0 37.0 6 35.48832773362375 37.0 35.0 37.0 33.0 37.0 7 35.42243575781449 37.0 35.0 37.0 33.0 37.0 8 35.410655513589916 37.0 35.0 37.0 33.0 37.0 9 37.02242787960238 39.0 37.0 39.0 33.0 39.0 10-11 36.90834881942674 39.0 37.0 39.0 33.0 39.0 12-13 36.781314271149526 39.0 37.0 39.0 33.0 39.0 14-15 37.91509419097713 40.0 38.0 41.0 33.0 41.0 16-17 37.844852283522954 40.0 38.0 41.0 33.0 41.0 18-19 37.79845772412355 40.0 38.0 41.0 33.0 41.0 20-21 37.72829413536622 40.0 37.5 41.0 32.0 41.0 22-23 37.529465671849294 40.0 37.0 41.0 32.0 41.0 24-25 37.35504460458327 40.0 37.0 41.0 32.0 41.0 26-27 37.303591769585466 39.5 37.0 41.0 32.0 41.0 28-29 37.344772806265496 40.0 37.0 41.0 32.0 41.0 30-31 37.16524387700721 39.0 36.5 41.0 31.0 41.0 32-33 36.928550640684016 39.0 36.0 41.0 31.0 41.0 34-35 36.93584887735477 39.0 36.0 41.0 31.0 41.0 36-37 36.84697453483791 39.0 36.0 41.0 30.5 41.0 38-39 36.71912528674375 39.0 36.0 41.0 30.0 41.0 40-41 36.48206988437565 39.0 35.5 41.0 30.0 41.0 42-43 36.23091340918043 39.0 35.0 41.0 30.0 41.0 44-45 35.97983988692448 39.0 35.0 41.0 29.0 41.0 46-47 35.611318210255575 39.0 35.0 40.5 27.5 41.0 48-49 35.24232708482981 39.0 35.0 40.0 26.0 41.0 50-51 34.314196769933034 38.0 34.0 39.5 24.5 40.5 52-53 34.424459994902335 38.0 34.0 39.5 24.0 40.5 54-55 34.77894246588039 38.0 34.0 40.0 24.5 41.0 56-57 34.58886252519869 38.0 34.0 40.0 24.0 41.0 58-59 34.349764348773085 38.0 34.0 40.0 23.0 41.0 60-61 34.03639409597516 38.0 34.0 40.0 22.5 41.0 62-63 33.67903422388025 37.5 33.0 40.0 21.5 41.0 64-65 33.30174826795189 37.0 33.0 40.0 20.0 41.0 66-67 32.898970271334896 36.5 32.0 39.0 18.5 41.0 68-69 32.411601362467266 36.0 31.5 39.0 17.0 40.5 70-71 31.96850429825984 35.0 31.0 39.0 13.0 40.0 72-73 31.50985703362143 35.0 31.0 38.0 8.0 40.0 74-75 31.03673865189888 35.0 30.5 37.0 7.0 39.0 76-77 30.53295803693491 35.0 30.0 37.0 4.0 39.0 78-79 29.98954584424311 34.0 29.5 36.0 2.0 39.0 80-81 29.43578770535487 34.0 29.0 36.0 2.0 37.5 82-83 28.898636605880853 34.0 29.0 35.0 2.0 37.0 84-85 28.425693166809555 34.0 27.5 35.0 2.0 36.5 86-87 27.92995435271219 33.5 26.5 35.0 2.0 36.0 88-89 27.430787821210927 33.0 26.0 35.0 2.0 36.0 90-91 26.908413467108463 33.0 25.5 35.0 2.0 35.0 92-93 26.319315754107095 32.0 24.5 35.0 2.0 35.0 94-95 25.661123340361932 32.0 22.0 34.0 2.0 35.0 96-97 24.908006812336353 32.0 19.0 34.0 2.0 35.0 98-99 23.93624139768751 31.0 2.0 34.0 2.0 35.0 100-101 21.570665940635354 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18067190692658386 1101 2 0.23717521167703737 1101 3 0.18801317117680938 1101 4 0.12356566097899702 1101 5 0.10214999966300553 1101 6 0.08997025851150653 1101 7 0.23135556769185683 1101 8 0.19818198361451778 1101 9 0.3665266818353956 1101 10-11 0.31070226586398064 1101 12-13 0.379871853902884 1101 14-15 0.4701982329856236 1101 16-17 0.5513307895259985 1101 18-19 0.48628999436088094 1101 20-21 0.5765053911294657 1101 22-23 0.5350946049018717 1101 24-25 0.4625557097423538 1101 26-27 0.4118589323408415 1101 28-29 0.33348367228764886 1101 30-31 0.5311222651213612 1101 32-33 0.3843224332134554 1101 34-35 0.5288662119987677 1101 36-37 0.5087435369075379 1101 38-39 0.565074738746155 1101 40-41 0.536647523524941 1101 42-43 0.4811106836579597 1101 44-45 0.5035143735764862 1101 46-47 0.7693206118338551 1101 48-49 0.8517095462671946 1101 50-51 0.805721093897084 1101 52-53 0.7950863090926461 1101 54-55 0.6478654408484843 1101 56-57 0.6316190518871991 1101 58-59 0.8263601597955912 1101 60-61 0.7914619798613529 1101 62-63 1.0065660124546483 1101 64-65 0.8239610264450334 1101 66-67 0.887192789814442 1101 68-69 0.8116977793800331 1101 70-71 0.9808506650967068 1101 72-73 1.0934293773291337 1101 74-75 1.1015593237616308 1101 76-77 1.0697010728291012 1101 78-79 1.1437016914899445 1101 80-81 1.1880929691231437 1101 82-83 1.1312065468013621 1101 84-85 1.4177127690367826 1101 86-87 1.440002089969468 1101 88-89 1.468907367436966 1101 90-91 1.440565911383036 1101 92-93 1.5484246891569988 1101 94-95 1.5747984016634184 1101 96-97 1.683538518557274 1101 98-99 2.0913778815736705 1101 100-101 2.6272864974146977 1102 1 0.13185838348755397 1102 2 -0.043176563256292155 1102 3 0.10724772027722551 1102 4 0.12901147231436738 1102 5 0.009066291326817577 1102 6 0.048700998641443505 1102 7 0.06339819351779852 1102 8 0.14046853271012338 1102 9 0.14398428876985037 1102 10-11 0.07156682337263476 1102 12-13 0.1593673171802763 1102 14-15 0.30504841829176854 1102 16-17 0.26019490076020446 1102 18-19 0.271101506557649 1102 20-21 0.4009284220186302 1102 22-23 0.3691746359847059 1102 24-25 0.4107893771199329 1102 26-27 0.3874909640203157 1102 28-29 0.25013665601126434 1102 30-31 0.34070471582968764 1102 32-33 0.2504994910980187 1102 34-35 0.36232960847211615 1102 36-37 0.5015885605865478 1102 38-39 0.4336784891355805 1102 40-41 0.45533580743729374 1102 42-43 0.5896637861074154 1102 44-45 0.7564425233899712 1102 46-47 0.7454013237558783 1102 48-49 0.5903826255729285 1102 50-51 0.5214782508281104 1102 52-53 0.44536276468713965 1102 54-55 0.47824506282635326 1102 56-57 0.6129440900490835 1102 58-59 0.5678340322910103 1102 60-61 0.7795292506224385 1102 62-63 0.8558759109662972 1102 64-65 0.7687408784166365 1102 66-67 0.7559593822624251 1102 68-69 0.8285228373896985 1102 70-71 0.8106210202036586 1102 72-73 0.8413393453681799 1102 74-75 0.9749146640789839 1102 76-77 0.8726170733920604 1102 78-79 1.0206824102684777 1102 80-81 1.028072002644251 1102 82-83 0.9586566657076396 1102 84-85 1.0909371741144476 1102 86-87 1.0290950401563386 1102 88-89 1.143160399035846 1102 90-91 1.169499892836786 1102 92-93 1.285046980986806 1102 94-95 1.511640492681753 1102 96-97 1.6384559277115507 1102 98-99 1.7216683689510788 1102 100-101 2.3450601592830154 1103 1 0.2806029286428213 1103 2 0.23642432016103854 1103 3 0.2864306352620467 1103 4 0.3288888826642875 1103 5 0.22414110522034747 1103 6 0.3550790811440194 1103 7 0.26247583284986575 1103 8 0.33209241140805545 1103 9 0.37261325524524835 1103 10-11 0.2229083219426684 1103 12-13 0.3004150713016287 1103 14-15 0.45902458567093163 1103 16-17 0.5270894239586781 1103 18-19 0.3918011475889571 1103 20-21 0.29045074923973146 1103 22-23 0.44068989434685335 1103 24-25 0.5096294387042022 1103 26-27 0.3531367714425855 1103 28-29 0.2579366527367526 1103 30-31 0.17049148643880585 1103 32-33 0.27076194054038183 1103 34-35 0.3366867218001559 1103 36-37 0.41508077513510244 1103 38-39 0.49133757939349465 1103 40-41 0.5150966538292181 1103 42-43 0.5487359318560721 1103 44-45 0.5769336187561223 1103 46-47 0.48459761747283636 1103 48-49 0.3989599687368255 1103 50-51 0.49774781239980825 1103 52-53 0.4685301765982999 1103 54-55 0.5480673677690575 1103 56-57 0.44671261225467873 1103 58-59 0.4035565920026656 1103 60-61 0.5733552698005653 1103 62-63 0.7527709399603779 1103 64-65 0.7267329459043879 1103 66-67 0.5860856645127441 1103 68-69 0.6458950304433984 1103 70-71 0.686310934171626 1103 72-73 0.578604113868753 1103 74-75 0.6133093302451122 1103 76-77 0.7344015553491907 1103 78-79 0.6851978376189791 1103 80-81 0.6111976089411435 1103 82-83 0.749635538069974 1103 84-85 0.8537848939703458 1103 86-87 1.0128788093217516 1103 88-89 1.043186829048647 1103 90-91 1.2130784230123162 1103 92-93 1.3119153987002967 1103 94-95 1.3618154307144827 1103 96-97 1.721636425987466 1103 98-99 1.9692665707746322 1103 100-101 2.537823466823699 1104 1 0.18297580134375835 1104 2 0.13890904144192007 1104 3 0.1413521468629675 1104 4 0.07671095434950814 1104 5 0.02023461585568498 1104 6 0.0378165947225213 1104 7 0.0906141792433246 1104 8 0.035300826367162585 1104 9 0.2072658022013414 1104 10-11 0.22724488432951517 1104 12-13 0.24638430090138286 1104 14-15 0.3896836886076045 1104 16-17 0.3895301050425459 1104 18-19 0.3178863544464505 1104 20-21 0.29655744959347174 1104 22-23 0.38308323345201956 1104 24-25 0.3648349238398012 1104 26-27 0.36302666659796756 1104 28-29 0.1886856987597625 1104 30-31 0.41211074923045743 1104 32-33 0.35331446150002677 1104 34-35 0.2531322783795744 1104 36-37 0.410578833524994 1104 38-39 0.497651656589035 1104 40-41 0.45790133748486994 1104 42-43 0.6060120519086212 1104 44-45 0.7589544890338189 1104 46-47 0.5725217716596589 1104 48-49 0.7390215043148274 1104 50-51 0.6857760829625406 1104 52-53 0.5890069823073034 1104 54-55 0.6822145071146934 1104 56-57 0.7160011247236895 1104 58-59 0.7195831588692627 1104 60-61 0.8027036118303315 1104 62-63 0.9306682098685499 1104 64-65 0.7728899244433904 1104 66-67 0.6965965586514642 1104 68-69 0.7532516785721128 1104 70-71 0.7215560029693826 1104 72-73 0.7321033786565501 1104 74-75 0.9398993499281438 1104 76-77 0.9717630284892813 1104 78-79 1.070119720597802 1104 80-81 1.1084159739267037 1104 82-83 1.1078651418793832 1104 84-85 1.1549867367010869 1104 86-87 1.3687358015553102 1104 88-89 1.3158040335943078 1104 90-91 1.3052388240308197 1104 92-93 1.4124605988729861 1104 94-95 1.7189110262885876 1104 96-97 1.9910259068243654 1104 98-99 1.9550543300428345 1104 100-101 2.3559510026384523 1105 1 0.25304445644032114 1105 2 0.18294662853262977 1105 3 0.256837880459873 1105 4 0.2481529233505455 1105 5 0.20089000070214524 1105 6 0.23937995775633425 1105 7 0.3024509756133753 1105 8 0.2432401923371046 1105 9 0.22893839806168614 1105 10-11 0.27988161916676546 1105 12-13 0.2087131022473443 1105 14-15 0.3763790382786212 1105 16-17 0.3384889985908117 1105 18-19 0.2938981026077343 1105 20-21 0.3925084886256158 1105 22-23 0.38055393875352905 1105 24-25 0.3117101206720676 1105 26-27 0.33447955221964065 1105 28-29 0.4414758719237071 1105 30-31 0.39357240448690334 1105 32-33 0.2527457373607547 1105 34-35 0.3440824907161826 1105 36-37 0.23855575509247728 1105 38-39 0.2914296521945303 1105 40-41 0.4521439178166631 1105 42-43 0.48380139232065034 1105 44-45 0.2805495632607844 1105 46-47 0.36712017931686347 1105 48-49 0.35100097380037454 1105 50-51 0.4421233938097515 1105 52-53 0.3978011605537688 1105 54-55 0.4683071109452328 1105 56-57 0.5061664910860912 1105 58-59 0.4232769284313562 1105 60-61 0.35617945778516 1105 62-63 0.4958206004419239 1105 64-65 0.4403801827600091 1105 66-67 0.5699109173537593 1105 68-69 0.5747684135547217 1105 70-71 0.6295815814745715 1105 72-73 0.4812034766487443 1105 74-75 0.31903615865320134 1105 76-77 0.3874297115081653 1105 78-79 0.5382440967909083 1105 80-81 0.5487699661696936 1105 82-83 0.5732870866791338 1105 84-85 0.7188226221919471 1105 86-87 0.8521798606122637 1105 88-89 0.7932503862014606 1105 90-91 0.9256566444374208 1105 92-93 1.1409269968787434 1105 94-95 1.1024554395224975 1105 96-97 1.2557484308560696 1105 98-99 1.5029414264458296 1105 100-101 1.690297567945585 1106 1 -0.07233984361931789 1106 2 0.018061672831489517 1106 3 0.05555693135860906 1106 4 -0.10371979833806932 1106 5 0.007850242550986763 1106 6 -0.1538675208958722 1106 7 -0.0831363812451471 1106 8 -0.2591005857869959 1106 9 -0.1351198235528983 1106 10-11 -0.4018828364614251 1106 12-13 -0.35661903504295367 1106 14-15 -0.3515394771419835 1106 16-17 -0.49397228014726835 1106 18-19 -0.1636270405359994 1106 20-21 -0.11382096315853829 1106 22-23 -0.2298031230131059 1106 24-25 -0.2669482840640569 1106 26-27 -0.22573143972600462 1106 28-29 -0.08666152730669552 1106 30-31 -0.04530622582019106 1106 32-33 -0.02301116292564842 1106 34-35 -0.0768657737500007 1106 36-37 -0.3556488645696234 1106 38-39 -0.17489169131238924 1106 40-41 -0.1727738677507773 1106 42-43 -0.2976044307425312 1106 44-45 -0.1820992500470382 1106 46-47 -0.15493880731801113 1106 48-49 0.029660983600280133 1106 50-51 -0.2052721377287554 1106 52-53 -0.0696597782354118 1106 54-55 -0.13140188558151777 1106 56-57 -0.15338784943017458 1106 58-59 -0.22307774629886268 1106 60-61 -0.2170122620334638 1106 62-63 0.032197928212717386 1106 64-65 0.02105077840639069 1106 66-67 0.09028729218555753 1106 68-69 -0.10884393937679704 1106 70-71 0.01099207545368941 1106 72-73 0.11690007703348826 1106 74-75 0.22167939211009013 1106 76-77 0.35842611887047227 1106 78-79 0.4040431813995937 1106 80-81 0.24211297316831804 1106 82-83 0.04509017384798497 1106 84-85 0.18022480590826362 1106 86-87 0.54252762022276 1106 88-89 0.5352217760297933 1106 90-91 0.7019497081633226 1106 92-93 0.8594530938631877 1106 94-95 1.0255299626337049 1106 96-97 1.1135672826367262 1106 98-99 1.408259165051632 1106 100-101 1.9523432559958458 1107 1 0.28751334338315004 1107 2 0.2817762450457231 1107 3 0.3633093455847707 1107 4 0.23802864270077606 1107 5 0.24115192523601792 1107 6 0.22992721256596127 1107 7 0.32089249604034364 1107 8 0.40888276742818164 1107 9 0.3972467118795251 1107 10-11 0.40546493526991156 1107 12-13 0.33173759513766754 1107 14-15 0.4450228145827708 1107 16-17 0.6074007143301756 1107 18-19 0.6087883089699417 1107 20-21 0.6480047573464702 1107 22-23 0.6706910749696746 1107 24-25 0.5289872660872916 1107 26-27 0.6439397196440311 1107 28-29 0.5976679509862421 1107 30-31 0.5541520359810193 1107 32-33 0.4924418852373691 1107 34-35 0.7257531231371033 1107 36-37 0.638801411760177 1107 38-39 0.6555771451582686 1107 40-41 0.6355443484668939 1107 42-43 0.7336059963870412 1107 44-45 0.7371850806427105 1107 46-47 0.7823869435650224 1107 48-49 0.9573918707355844 1107 50-51 0.8721880363271737 1107 52-53 0.9430813667569211 1107 54-55 1.028229440961212 1107 56-57 1.0889704279546777 1107 58-59 1.1711225125475195 1107 60-61 1.1149710698895703 1107 62-63 1.1081524173582196 1107 64-65 1.1783909051570447 1107 66-67 1.141283167934155 1107 68-69 1.1080721543446117 1107 70-71 1.4967828739072182 1107 72-73 1.3481065944850634 1107 74-75 1.3000219052327502 1107 76-77 1.3439610445012349 1107 78-79 1.5481029763452412 1107 80-81 1.5098298258444913 1107 82-83 1.561153260265268 1107 84-85 1.5055519569332496 1107 86-87 1.767480121306182 1107 88-89 1.775970477530283 1107 90-91 1.8709365453971856 1107 92-93 1.7993683180942348 1107 94-95 1.8733266571753369 1107 96-97 2.1971333458240956 1107 98-99 2.427804159296329 1107 100-101 2.88322914314125 1108 1 0.036935312128459685 1108 2 0.009755062285957905 1108 3 0.021071082295815557 1108 4 0.004606067989307405 1108 5 0.08653551701525686 1108 6 0.0729183403728797 1108 7 -0.04990000180462317 1108 8 0.011300652582896475 1108 9 0.041128516409337124 1108 10-11 -0.02800689507024856 1108 12-13 0.024632508278898513 1108 14-15 0.11456556567915754 1108 16-17 0.22007242214090894 1108 18-19 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-0.1214414987784096 2316 86-87 -0.3771474793335834 2316 88-89 -0.48861025198354824 2316 90-91 -0.6674998677868942 2316 92-93 -0.743078834727104 2316 94-95 -0.5305265787594458 2316 96-97 -0.1854904556009096 2316 98-99 0.012873574604206794 2316 100-101 0.41996464933326294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9026.0 3 1671.0 4 1560.0 5 1808.0 6 2040.0 7 2470.0 8 3011.0 9 3849.0 10 4736.0 11 6263.0 12 8270.0 13 10590.0 14 11929.0 15 14905.0 16 19088.0 17 24977.0 18 21768.0 19 14999.0 20 14804.0 21 15617.0 22 17061.0 23 18802.0 24 21171.0 25 24131.0 26 27847.0 27 32347.0 28 38129.0 29 46137.0 30 56153.0 31 69211.0 32 87241.0 33 113511.0 34 152391.0 35 210852.0 36 300035.0 37 403412.0 38 309910.0 39 36125.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21180460307733 19.465999510884675 14.869570796653559 22.45262508938443 2 14.733340058190155 20.183309680121493 41.70715913691886 23.37619112476949 3 18.868949682552962 25.20812999061138 30.32274556829062 25.60017475854504 4 12.104301226671426 16.722872261574658 38.02960238782 33.14322412393392 5 13.046801811179348 38.051541658612024 34.42174790703207 14.479908623176552 6 31.168982572762122 36.55470088606225 17.86820213013508 14.408114411040549 7 27.85959436928464 33.52644216255881 20.86271996433199 17.751243503824558 8 26.050899882158568 35.598935943402665 19.81830959550707 18.531854578931696 9 26.30943317699859 16.972918337893937 20.867274633406087 35.85037385170139 10-11 24.406553551388672 27.29644638172319 27.550063915334476 20.746936151553662 12-13 25.319702657484793 24.726443585463215 28.392875224460713 21.560978532591278 14-15 22.326280578092458 25.772568847735172 26.991628472112556 24.90952210205981 16-17 21.136746817276116 29.44043975234901 27.486774452747987 21.93603897762688 18-19 21.01379840799033 27.574382188219055 30.44978592681289 20.962033476977727 20-21 21.779970691489968 26.65388628010177 30.628066369773453 20.938076658634806 22-23 21.64070758776876 26.69964576032669 30.45890023406241 21.200746417842144 24-25 21.336720099559784 26.79551237996174 30.723410479781567 21.144357040696917 26-27 21.1933132436815 27.130806204678983 30.402104978145132 21.273775573494387 28-29 20.918357037896875 27.056599943656227 30.77984100651437 21.245202011932523 30-31 21.04683670298668 26.809378209198275 30.807769284683495 21.33601580313155 32-33 20.865245199488403 27.01907849035903 30.591752077866307 21.523924232286255 34-35 21.385414370953498 26.918418878814908 30.446306379909306 21.249860370322292 36-37 21.446452135118236 26.611058957437884 30.351053946660528 21.591434960783353 38-39 21.115643124603228 26.944935803871505 30.538172861201033 21.401248210324226 40-41 21.3045924289273 26.969348901864986 30.40319320679664 21.32286546241107 42-43 21.258841063124343 26.86059996386711 30.228296696534358 21.65226227647419 44-45 21.15272533236242 27.203748484301048 29.99572283804789 21.647803345288644 46-47 21.367646201719374 27.273231787639666 29.56858676528195 21.790535245359006 48-49 21.5124795985572 27.458321605696888 29.27243601355111 21.756762782194798 50-51 21.521046121288496 27.598453596994148 29.055742876824254 21.824757404893102 52-53 21.65622228530095 27.69246922085953 28.97680525042125 21.674503243418275 54-55 21.403519934755646 27.588589947734388 28.999531613532163 22.008358503977803 56-57 21.467324308174042 28.00155727874593 28.902912445554442 21.628205967525584 58-59 21.682430099943286 27.579504139598836 28.94376214004132 21.794303620416557 60-61 21.578053891562167 27.527707985759797 29.275740717610017 21.618497405068023 62-63 21.538331154613275 27.483241656817924 29.440212129162884 21.538215059405918 64-65 21.490004341396258 27.435093804340006 29.260964347153834 21.813937507109905 66-67 21.38470339724834 27.803980149865083 28.943965207005963 21.867351245880613 68-69 21.27304034315153 27.750944878708637 29.155968721833002 21.82004605630683 70-71 21.341515510599805 27.65777591631594 29.16632615535488 21.834382417729373 72-73 21.4443062232506 27.70841952810077 28.8416166214623 22.005657627186327 74-75 21.300081054229715 27.996469926930462 28.73610428435802 21.967344734481806 76-77 21.46432763747447 27.800420077815406 28.775516127946087 21.95973615676403 78-79 21.475820081958265 27.818180467031144 28.68105062267568 22.024948828334914 80-81 21.264399043686154 28.113801904825596 28.72786852356511 21.89393052792314 82-83 21.43371637474156 27.990514059402997 28.794956745299753 21.78081282055569 84-85 21.394619454627016 27.872258716429638 28.565444851128092 22.16767697781526 86-87 21.524692101884828 27.638515550645565 28.804538245121798 22.03225410234781 88-89 21.711847727603086 27.595122646692356 28.713429950972902 21.979599674731656 90-91 21.392170125694378 27.683181715695394 28.88181904941697 22.04282910919326 92-93 21.52352717587281 27.657187809906464 28.682695949119786 22.136589065100935 94-95 21.289656551180638 27.614921597688436 28.66969745027338 22.425724400857547 96-97 21.405555347894715 27.559918342083336 28.575743568324658 22.458782741697288 98-99 21.317643890195566 27.67962330476697 28.48379239969073 22.518940405346733 100-101 21.434955944518325 27.19418400778586 29.009638529601865 22.361221518093952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3063.0 1 1792.5 2 545.0 3 560.5 4 479.5 5 440.0 6 759.5 7 1733.5 8 2927.0 9 3591.5 10 3404.0 11 3042.0 12 3133.5 13 3327.0 14 3545.0 15 3735.0 16 3871.0 17 3979.5 18 4052.0 19 4246.5 20 4689.5 21 5422.5 22 6632.5 23 8038.0 24 9694.0 25 12042.0 26 14974.5 27 18263.5 28 20677.0 29 23179.0 30 27695.0 31 32030.5 32 36017.0 33 41622.5 34 48606.0 35 53699.0 36 58872.0 37 63898.0 38 66358.5 39 67527.5 40 69141.0 41 73775.0 42 77905.5 43 81287.5 44 85014.5 45 87777.0 46 86188.5 47 83650.5 48 84987.5 49 82813.0 50 77157.0 51 71399.0 52 66069.5 53 63150.0 54 60406.5 55 55109.5 56 48602.0 57 42621.5 58 37887.5 59 33379.5 60 27904.5 61 22199.5 62 17152.0 63 13150.5 64 10029.5 65 7710.5 66 5686.0 67 4298.0 68 3144.5 69 2076.5 70 1491.5 71 1132.5 72 809.0 73 580.5 74 447.0 75 348.0 76 245.0 77 164.5 78 93.0 79 58.5 80 40.0 81 28.0 82 25.0 83 19.5 84 13.0 85 9.5 86 10.5 87 6.0 88 2.0 89 3.0 90 3.0 91 3.0 92 2.0 93 1.5 94 2.0 95 1.0 96 0.5 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325694557082281 2 0.30711124498922543 3 0.29242069652663527 4 0.2747642329170239 5 0.2725861389809301 6 0.2243436754176611 7 0.21595569664249137 8 0.2297193966216373 9 0.23129503904349238 10-11 0.23180480570938666 12-13 0.22763398753388792 14-15 0.2242046481451445 16-17 0.2233704845100447 18-19 0.22466807238686656 20-21 0.21088120119563455 22-23 0.20226151029960376 24-25 0.20251639363255092 26-27 0.21634960724795516 28-29 0.23145723752809508 30-31 0.22517783905276084 32-33 0.2308084435896842 34-35 0.22399610723636953 36-37 0.20814699816947424 38-39 0.20979215422758765 40-41 0.2040688648423199 42-43 0.217206942095141 44-45 0.21099705725606505 46-47 0.21162267998238987 48-49 0.22318511481335587 50-51 0.23092429965011468 52-53 0.24732951780707652 54-55 0.21843501633570453 56-57 0.18967954213684918 58-59 0.18900757698635218 60-61 0.19598211182426953 62-63 0.20599207544546658 64-65 0.19301619667724818 66-67 0.19987487545473503 68-69 0.20386032393354497 70-71 0.1958430845517529 72-73 0.19913339666797972 74-75 0.20175174363370948 76-77 0.216766689065505 78-79 0.2225826632991172 80-81 0.2123409875570591 82-83 0.2112287693769261 84-85 0.20335055726765067 86-87 0.20476400120490304 88-89 0.21090437240772064 90-91 0.2118543921032509 92-93 0.20754454665523553 94-95 0.20861042241119634 96-97 0.19609796788470005 98-99 0.20321152999513403 100-101 0.1947308663716199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2157850.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.52619023021309 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96589137734843 37.87740888509398 2 13.527353796371147 13.669713024536875 3 4.7157204068476375 7.1480215904664455 4 2.182306997735831 4.410546340333031 5 1.1642526511073754 2.9412625462940576 6 0.7305489840302649 2.2147114163761237 7 0.4717780937923443 1.6686004799379512 8 0.3462356761607007 1.3995175710545615 9 0.24511401752437095 1.1146209729775334 >10 1.4409422647125107 13.60485756081622 >50 0.1334580410523142 4.677460977517941 >100 0.07291000463041947 6.638053307540215 >500 0.0025698758744905357 0.8394082575761938 >1k 6.424689686226253E-4 0.5824985980561548 >5k 1.8356256246360725E-4 0.6899925675087595 >10k+ 9.178128123180363E-5 0.5233259039139962 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11285 0.5229742567833724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8896 0.41226220543596637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5983 0.27726672382232315 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3194 0.14801770280603377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.3171212086104225E-5 0.0 18-19 0.0 0.0 0.0 4.634242417220845E-5 0.0 20-21 0.0 0.0 0.0 1.1585606043052113E-4 0.0 22-23 0.0 0.0 0.0 3.243969692054592E-4 0.0 24-25 0.0 0.0 0.0 4.634242417220845E-4 4.634242417220845E-5 26-27 0.0 0.0 0.0 6.487939384109184E-4 4.634242417220845E-5 28-29 0.0 0.0 0.0 0.001251245452649628 4.634242417220845E-5 30-31 0.0 0.0 0.0 0.0024329772690409434 4.634242417220845E-5 32-33 0.0 0.0 0.0 0.004078133327154343 9.26848483444169E-5 34-35 0.0 0.0 0.0 0.005769631809439952 9.26848483444169E-5 36-37 0.0 0.0 0.0 0.008434321199341937 9.26848483444169E-5 38-39 0.0 0.0 0.0 0.011122181801330028 9.26848483444169E-5 40-41 0.0 0.0 0.0 0.014574692402159556 9.26848483444169E-5 42-43 0.0 0.0 0.0 0.02013578330282457 9.26848483444169E-5 44-45 0.0 0.0 0.0 0.02704080450448363 9.26848483444169E-5 46-47 0.0 0.0 0.0 0.04339968023727321 1.1585606043052113E-4 48-49 0.0 0.0 0.0 0.07162221655814816 1.3902727251662535E-4 50-51 0.0 0.0 0.0 0.09599833167272981 1.3902727251662535E-4 52-53 0.0 0.0 0.0 0.12102324072572238 1.3902727251662535E-4 54-55 0.0 0.0 0.0 0.15218852098153254 1.3902727251662535E-4 56-57 0.0 0.0 0.0 0.17807076488171097 1.3902727251662535E-4 58-59 0.0 0.0 0.0 0.20872627847162684 1.3902727251662535E-4 60-61 0.0 0.0 0.0 0.2570614268832403 1.3902727251662535E-4 62-63 0.0 0.0 0.0 0.29390365410014596 1.3902727251662535E-4 64-65 0.0 0.0 0.0 0.3375582176703663 1.3902727251662535E-4 66-67 0.0 0.0 0.0 0.41379150543364923 1.3902727251662535E-4 68-69 0.0 0.0 0.0 0.49533100076465 1.3902727251662535E-4 70-71 0.0 0.0 0.0 0.5684130036842228 1.3902727251662535E-4 72-73 0.0 0.0 0.0 0.6546562550687026 1.3902727251662535E-4 74-75 0.0 0.0 0.0 0.7414787867553352 1.3902727251662535E-4 76-77 0.0 0.0 0.0 0.8150473851287161 1.3902727251662535E-4 78-79 0.0 0.0 0.0 0.9112310864981348 1.3902727251662535E-4 80-81 0.0 0.0 0.0 1.0503973862872766 1.3902727251662535E-4 82-83 0.0 2.3171212086104225E-5 0.0 1.178951270940983 1.3902727251662535E-4 84-85 0.0 4.634242417220845E-5 0.0 1.321709108603471 1.3902727251662535E-4 86-87 0.0 4.634242417220845E-5 0.0 1.5561090900665016 1.3902727251662535E-4 88-89 0.0 4.634242417220845E-5 0.0 1.8565238547628427 1.3902727251662535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5470 0.0 53.891083 1 GTATCAA 9520 0.0 41.053238 1 TATCAAC 12205 0.0 31.389807 2 ATCAACG 12185 0.0 31.358934 3 TCAACGC 12320 0.0 31.053165 4 CAACGCA 12560 0.0 30.459793 5 AACGCAG 12940 0.0 29.700476 6 GTACATG 10290 0.0 26.891817 1 TACATGG 10335 0.0 26.445316 2 ACGCAGA 14840 0.0 25.673769 7 CGCAGAG 14760 0.0 25.651993 8 ACATGGG 10335 0.0 25.10805 3 CGACACG 115 1.2777164E-4 24.795574 4 CATGGGG 4095 0.0 24.139605 4 GCAGAGT 15465 0.0 23.80458 9 GAGTACT 8850 0.0 23.240385 12-13 TGGTATC 2455 0.0 21.49131 2 CAGAGTA 15225 0.0 21.043028 10-11 GTACTTT 9810 0.0 20.91719 14-15 AGTACTT 9400 0.0 20.693062 12-13 >>END_MODULE