FastQCFastQC Report
Thu 26 May 2016
SRR937733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157850
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127210.5895219778946637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105340.48817109623004384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75530.3500243297726904No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA29040.13457839979609335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.12350256041893551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68400.047.799171
GTATCAA113150.036.4546741
ATCAACG141750.028.6865063
TATCAAC144400.028.3574432
TCAACGC144050.028.1955014
CAACGCA147250.027.5827645
AACGCAG151050.026.9486336
TAACGCA2151.6625563E-924.3041924
GTACATG103900.023.6005541
ACGCAGA174350.023.1041957
CGCAGAG174800.022.8544868
TACATGG106700.022.5275022
ACCGTGC3600.022.431768
GAGTACT109450.021.85247412-13
ACATGGG107900.021.528533
TGGTATC34750.021.4620822
ATACCGT2005.392649E-721.3741656
GCAGAGT185000.021.2606019
ATATGCG1150.003722663520.6537635
GTACTTT115950.020.6050614-15
Produced by FastQC (version 0.11.5)