##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937733_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2157850 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25754941260978 33.0 31.0 34.0 31.0 34.0 2 32.46677062817156 34.0 31.0 34.0 31.0 34.0 3 32.52766179298839 34.0 31.0 34.0 31.0 34.0 4 36.1339875338879 37.0 35.0 37.0 35.0 37.0 5 36.05480408740181 37.0 35.0 37.0 35.0 37.0 6 36.106899923535 37.0 35.0 37.0 35.0 37.0 7 36.05672127348981 37.0 35.0 37.0 35.0 37.0 8 36.023682369024726 37.0 35.0 37.0 35.0 37.0 9 37.71311166207104 39.0 37.0 39.0 35.0 39.0 10-11 37.63635632689946 39.0 37.0 39.0 35.0 39.0 12-13 37.530059086590825 39.0 37.0 39.0 35.0 39.0 14-15 38.74211089742104 40.0 38.0 41.0 35.0 41.0 16-17 38.70405009616053 40.0 38.0 41.0 34.5 41.0 18-19 38.696908496883474 40.0 38.0 41.0 34.5 41.0 20-21 38.6001466737725 40.0 38.0 41.0 34.0 41.0 22-23 38.5468732766411 40.0 38.0 41.0 34.0 41.0 24-25 38.56229673054197 40.0 38.0 41.0 34.0 41.0 26-27 38.39328590958593 40.0 38.0 41.0 34.0 41.0 28-29 38.35733762773131 40.0 38.0 41.0 34.0 41.0 30-31 38.36283430266237 40.0 38.0 41.0 34.0 41.0 32-33 38.23611372430892 40.0 38.0 41.0 34.0 41.0 34-35 38.08420650184211 40.0 37.5 41.0 34.0 41.0 36-37 37.9103510438631 40.0 37.0 41.0 33.0 41.0 38-39 37.77236948814792 40.0 37.0 41.0 33.0 41.0 40-41 37.58767847626109 40.0 37.0 41.0 33.0 41.0 42-43 37.351824964663905 40.0 37.0 41.0 32.5 41.0 44-45 37.02469356072016 40.0 36.0 41.0 31.5 41.0 46-47 36.64693305836828 40.0 36.0 41.0 30.5 41.0 48-49 36.2821259587089 39.0 36.0 41.0 30.0 41.0 50-51 36.03918367819821 39.0 35.5 41.0 29.5 41.0 52-53 35.77913316495586 39.0 35.0 40.0 28.5 41.0 54-55 35.54430938202377 39.0 35.0 40.0 28.0 41.0 56-57 35.28399124128183 38.5 35.0 40.0 27.5 41.0 58-59 35.03322798155571 38.0 34.5 40.0 27.0 41.0 60-61 34.70572097226406 38.0 34.0 40.0 26.0 41.0 62-63 34.30592279352133 37.5 34.0 40.0 26.0 41.0 64-65 33.90871260745649 37.0 33.5 39.5 25.0 41.0 66-67 33.498079569942306 36.5 33.0 39.0 23.5 40.5 68-69 33.03122830595268 36.0 32.0 39.0 22.5 40.0 70-71 32.61089464049864 35.5 32.0 38.5 22.0 40.0 72-73 32.135838450309336 35.0 31.5 38.0 20.5 40.0 74-75 31.644090877493802 35.0 31.0 37.0 19.5 39.0 76-77 30.372323377435876 33.5 29.5 35.5 18.5 38.5 78-79 30.990382788423663 34.0 31.0 36.0 18.0 38.5 80-81 30.81325022591932 34.0 31.0 36.0 18.0 37.5 82-83 30.41828903769956 34.0 30.5 35.0 14.5 37.0 84-85 30.00567578840049 34.0 30.0 35.0 8.5 36.5 86-87 29.645926500915266 34.0 30.0 35.0 7.0 36.0 88-89 29.232092128739254 34.0 29.0 35.0 2.0 36.0 90-91 28.726831336747225 34.0 29.0 35.0 2.0 35.0 92-93 28.264544106402205 33.5 29.0 35.0 2.0 35.0 94-95 27.761960284542486 33.0 27.0 35.0 2.0 35.0 96-97 27.230901128438028 33.0 26.5 35.0 2.0 35.0 98-99 26.387753782700372 32.5 25.0 34.0 2.0 35.0 100-101 23.91808976527562 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2068538004925884 1101 2 0.17207465137300204 1101 3 0.2177632932751976 1101 4 0.13905082583764994 1101 5 0.08607497886521287 1101 6 0.10878334676201717 1101 7 0.13268598260331999 1101 8 0.13987631163707448 1101 9 0.20575879887552873 1101 10-11 0.15656755440205217 1101 12-13 0.17056334848520294 1101 14-15 0.28138481952280614 1101 16-17 0.32735778121730874 1101 18-19 0.31869381745212166 1101 20-21 0.33029995083334995 1101 22-23 0.3462084962426246 1101 24-25 0.1662413851489717 1101 26-27 0.09228308282227005 1101 28-29 0.1267390351209059 1101 30-31 0.1923622841503274 1101 32-33 0.20842679733370062 1101 34-35 0.2296654838376142 1101 36-37 0.16997130418388195 1101 38-39 0.21777617327132504 1101 40-41 0.21256490616384838 1101 42-43 0.27656492249271736 1101 44-45 0.20274288925340755 1101 46-47 0.23144478365767895 1101 48-49 0.19825896336795523 1101 50-51 0.23552010717255456 1101 52-53 0.24595730745145516 1101 54-55 0.31736749941702413 1101 56-57 0.3548135346718553 1101 58-59 0.2810046131082444 1101 60-61 0.38323401662341183 1101 62-63 0.4284312815615934 1101 64-65 0.5422965144052299 1101 66-67 0.48667889116364194 1101 68-69 0.47247860441163425 1101 70-71 0.5541510149618531 1101 72-73 0.4876599971823552 1101 74-75 0.43201386590296664 1101 76-77 0.42427187340325645 1101 78-79 0.48016742537481605 1101 80-81 0.6124716190010417 1101 82-83 0.7418379929485894 1101 84-85 0.7774352263722761 1101 86-87 0.7027642205520621 1101 88-89 0.9126861398209165 1101 90-91 0.6597571743201307 1101 92-93 0.7722985289014552 1101 94-95 0.8292106825822181 1101 96-97 1.026708659985843 1101 98-99 1.0602903076850012 1101 100-101 1.4816636161386718 1102 1 0.06651762712158416 1102 2 0.11525014068106287 1102 3 0.15690607343270813 1102 4 0.10785491857132001 1102 5 0.0124893140185236 1102 6 0.09823274513353653 1102 7 0.07466609391932622 1102 8 0.09401680027752946 1102 9 0.13174490301986452 1102 10-11 0.09148035044982095 1102 12-13 0.10488958232579648 1102 14-15 0.08600306841885441 1102 16-17 0.15050378754214222 1102 18-19 0.19238386360724746 1102 20-21 0.13399332299616873 1102 22-23 0.2198874156088948 1102 24-25 0.15000995636102488 1102 26-27 0.09952602092003104 1102 28-29 0.24724291025067657 1102 30-31 0.18019499017334084 1102 32-33 0.13280914026253043 1102 34-35 0.25787369793386716 1102 36-37 0.3223024566666055 1102 38-39 0.19830278915171817 1102 40-41 0.23964330010510082 1102 42-43 0.22853824346027096 1102 44-45 0.36470142061350685 1102 46-47 0.3339852099881142 1102 48-49 0.39261028360655814 1102 50-51 0.3610191586889826 1102 52-53 0.48361439064495215 1102 54-55 0.480409689794584 1102 56-57 0.6676938753801451 1102 58-59 0.6993166912676188 1102 60-61 0.6201063604784807 1102 62-63 0.5573715180750227 1102 64-65 0.839035192446488 1102 66-67 0.7088841725992339 1102 68-69 0.6924934120625323 1102 70-71 0.6083732103193924 1102 72-73 0.628038422278614 1102 74-75 0.7353603529050687 1102 76-77 0.5971618733671704 1102 78-79 0.5716345917245178 1102 80-81 0.729652192950887 1102 82-83 0.7153138333820195 1102 84-85 0.6965379079885352 1102 86-87 0.5887604951828642 1102 88-89 0.5836185767560806 1102 90-91 0.6195769864999363 1102 92-93 0.8695325294812513 1102 94-95 1.0136388618666636 1102 96-97 0.9447385086494826 1102 98-99 0.923546583494641 1102 100-101 1.3638643025111818 1103 1 0.10264532033696128 1103 2 0.12174435151420937 1103 3 0.12098011372210493 1103 4 0.08297094457330445 1103 5 0.14863267812645375 1103 6 0.10905297017457372 1103 7 0.10327512093816438 1103 8 0.1256812127915552 1103 9 0.1080165111633491 1103 10-11 0.18791466689776826 1103 12-13 0.2408369871850553 1103 14-15 0.17693101038041448 1103 16-17 0.183657519582745 1103 18-19 0.1937984101717234 1103 20-21 0.20337707684153372 1103 22-23 0.2722143823497589 1103 24-25 0.08689258605373595 1103 26-27 0.10236549309163223 1103 28-29 0.19845066535438605 1103 30-31 0.2218795643431548 1103 32-33 0.31519260115576486 1103 34-35 0.36337812817705384 1103 36-37 0.29239180999084624 1103 38-39 0.37351096935762484 1103 40-41 0.35401055565817785 1103 42-43 0.3313194906645123 1103 44-45 0.5344826771735072 1103 46-47 0.5900904990897686 1103 48-49 0.6353364751060369 1103 50-51 0.5816705883363298 1103 52-53 0.45568102468342175 1103 54-55 0.6186314206446681 1103 56-57 0.6655257518467295 1103 58-59 0.6125370041358096 1103 60-61 0.6153507854707456 1103 62-63 0.41464374779160096 1103 64-65 0.40453594178412544 1103 66-67 0.5873865147544564 1103 68-69 0.6362780320170742 1103 70-71 0.5942172925401294 1103 72-73 0.5808180906989051 1103 74-75 0.6772307704115619 1103 76-77 0.5362056828675925 1103 78-79 0.7047100716309842 1103 80-81 0.6525294009825693 1103 82-83 0.7664437749026902 1103 84-85 0.7450870493931987 1103 86-87 0.8085411816264312 1103 88-89 0.8939072193936575 1103 90-91 0.9436404460131911 1103 92-93 1.11140325397351 1103 94-95 1.1487867880754727 1103 96-97 1.1479848685291927 1103 98-99 1.2295222222735518 1103 100-101 1.6759977834315798 1104 1 0.09465503767195571 1104 2 0.11439193941471615 1104 3 0.1049351375367138 1104 4 0.053864926626467025 1104 5 0.09893172438157194 1104 6 0.04641167248733069 1104 7 0.04742786286765721 1104 8 0.06793064295857931 1104 9 0.04111510271264507 1104 10-11 0.07414304574036379 1104 12-13 0.08183610690336707 1104 14-15 0.11736663666282965 1104 16-17 0.08481246134603992 1104 18-19 0.21843636023532298 1104 20-21 0.011833699902247474 1104 22-23 0.01361850708167367 1104 24-25 0.01026832997010274 1104 26-27 0.09415231114895306 1104 28-29 0.1264756689521107 1104 30-31 0.16326278456096333 1104 32-33 0.06369671706745095 1104 34-35 -0.0033238967609676706 1104 36-37 -0.053260994028391906 1104 38-39 0.03675781612709983 1104 40-41 0.07610034555112577 1104 42-43 0.054619015068681165 1104 44-45 0.1183435073656014 1104 46-47 0.23949408938565853 1104 48-49 0.26530437993116607 1104 50-51 0.2781722955141319 1104 52-53 0.3173378952907129 1104 54-55 0.3750224572068177 1104 56-57 0.3039128372961457 1104 58-59 0.25916715584369143 1104 60-61 0.1971981236196214 1104 62-63 0.2624116650346693 1104 64-65 0.4419880253302324 1104 66-67 0.4227961068654622 1104 68-69 0.3056287502851589 1104 70-71 0.3592472174753638 1104 72-73 0.3548336200981055 1104 74-75 0.44755500653363356 1104 76-77 0.4098998398165392 1104 78-79 0.30606403423322703 1104 80-81 0.3584295078697721 1104 82-83 0.2990039700385694 1104 84-85 0.21085014104462374 1104 86-87 0.3566849594217487 1104 88-89 0.3574465407701659 1104 90-91 0.25953579752237843 1104 92-93 0.4732803400728365 1104 94-95 0.42210373532588363 1104 96-97 0.6592809222907867 1104 98-99 1.0337991030705282 1104 100-101 1.1914009721751135 1105 1 0.11333063507304075 1105 2 0.11602322472339921 1105 3 0.13273724176455204 1105 4 0.07182526583206084 1105 5 0.05981221872733755 1105 6 0.08047607116171207 1105 7 0.06503481243984055 1105 8 0.07242674628523105 1105 9 0.10080918296691266 1105 10-11 0.09050495548580528 1105 12-13 0.0905239048771449 1105 14-15 0.07268510522296623 1105 16-17 0.21065558481377877 1105 18-19 0.28583892220417795 1105 20-21 0.2558760412493726 1105 22-23 0.1572476974893391 1105 24-25 0.0867170760576812 1105 26-27 0.06248298270433139 1105 28-29 -0.040307507278789956 1105 30-31 0.04704877155671028 1105 32-33 0.14683450510877094 1105 34-35 0.2049391825921063 1105 36-37 0.20323469807021866 1105 38-39 0.2605550498160696 1105 40-41 0.28759510799577015 1105 42-43 0.2504924113398914 1105 44-45 0.1756679541760704 1105 46-47 0.19422932878214993 1105 48-49 0.3691380972388387 1105 50-51 0.3170371304899575 1105 52-53 0.36862489067333115 1105 54-55 0.42193016164590347 1105 56-57 0.19625656155653814 1105 58-59 0.3246714219560758 1105 60-61 0.5170744887271255 1105 62-63 0.3971270788790733 1105 64-65 0.4529625600793068 1105 66-67 0.3846484227031297 1105 68-69 0.36245884527338035 1105 70-71 0.3916265804897989 1105 72-73 0.4498874067876457 1105 74-75 0.4136084044037247 1105 76-77 0.4308661863430814 1105 78-79 0.5581477309492193 1105 80-81 0.5629395257239729 1105 82-83 0.4980918644298775 1105 84-85 0.696134430437727 1105 86-87 0.7683107515959406 1105 88-89 0.8587449524245763 1105 90-91 0.8876259127873567 1105 92-93 0.8802023708731461 1105 94-95 0.9965822660729025 1105 96-97 0.9462887961873143 1105 98-99 1.1248313036189934 1105 100-101 1.3199491009966877 1106 1 -0.398912665183353 1106 2 -0.34294277876337986 1106 3 -0.33011013055668315 1106 4 -0.23820578764343026 1106 5 -0.33814116264609595 1106 6 -0.35670651169610323 1106 7 -0.4325576820655215 1106 8 -0.381641828892441 1106 9 -0.6657618832156373 1106 10-11 -0.6236415716881964 1106 12-13 -0.7555411910993541 1106 14-15 -0.7229619618115422 1106 16-17 -0.553161895268552 1106 18-19 -0.6958835492838489 1106 20-21 -0.691397343132202 1106 22-23 -0.7818126296442998 1106 24-25 -0.8500582894955926 1106 26-27 -0.7338864532023237 1106 28-29 -0.8653492434456211 1106 30-31 -0.8522046213046437 1106 32-33 -0.9426050589198383 1106 34-35 -1.2494032453211403 1106 36-37 -1.065228720271982 1106 38-39 -1.1590168273745505 1106 40-41 -1.2519404081882328 1106 42-43 -1.378214226464479 1106 44-45 -1.3138231161688267 1106 46-47 -1.2051358775109264 1106 48-49 -1.2317127488732353 1106 50-51 -1.420359466883042 1106 52-53 -1.4737705606119889 1106 54-55 -1.3215994926287706 1106 56-57 -1.6105144991796152 1106 58-59 -1.587464862025513 1106 60-61 -1.5228873612513425 1106 62-63 -1.690101554881629 1106 64-65 -2.1663084668423025 1106 66-67 -2.115872321303442 1106 68-69 -2.1076250202107296 1106 70-71 -2.047417786178187 1106 72-73 -2.1876640341538547 1106 74-75 -2.3958881087304746 1106 76-77 -2.1348261885760103 1106 78-79 -2.1734127463210022 1106 80-81 -2.3135748023557525 1106 82-83 -2.536144867328435 1106 84-85 -2.3111487718845325 1106 86-87 -2.1468058112014603 1106 88-89 -2.3436137338830214 1106 90-91 -2.6365401258304786 1106 92-93 -2.6200597594865265 1106 94-95 -2.47069030315269 1106 96-97 -2.515319971861313 1106 98-99 -2.4946183542769944 1106 100-101 -1.9090804251433973 1107 1 0.14997767458969236 1107 2 0.18751058419295674 1107 3 0.12883687384987041 1107 4 0.1287149224348525 1107 5 0.11224548248124222 1107 6 0.11321824444002715 1107 7 0.11680071774915746 1107 8 0.06734797822827687 1107 9 0.16859185160063817 1107 10-11 0.1189489001879096 1107 12-13 0.14282920738860838 1107 14-15 0.2399145443093076 1107 16-17 0.2698021949815015 1107 18-19 0.2538600180568693 1107 20-21 0.22185497849149982 1107 22-23 0.18466096378480046 1107 24-25 0.1825179551907823 1107 26-27 0.3475583802316322 1107 28-29 0.29754648066987954 1107 30-31 0.2971853679740235 1107 32-33 0.30198586486364576 1107 34-35 0.32936795573377964 1107 36-37 0.23983546911502174 1107 38-39 0.3172901568705484 1107 40-41 0.23588085308123397 1107 42-43 0.3139242370761224 1107 44-45 0.31758363920409494 1107 46-47 0.16346874739952 1107 48-49 0.22618570926356796 1107 50-51 0.2817048063876655 1107 52-53 0.3455037480862728 1107 54-55 0.2845691899694387 1107 56-57 0.3607902998675243 1107 58-59 0.4585617762809946 1107 60-61 0.4290968370416479 1107 62-63 0.4024620907297418 1107 64-65 0.36399640177140924 1107 66-67 0.4619888241881611 1107 68-69 0.4655606643472652 1107 70-71 0.4059887639825277 1107 72-73 0.48115228238248875 1107 74-75 0.5899471095387696 1107 76-77 0.48731958587888613 1107 78-79 0.6490059163158186 1107 80-81 0.6601327966816228 1107 82-83 0.5915475103963814 1107 84-85 0.6557222136975653 1107 86-87 0.7655715134738017 1107 88-89 0.8491418347007169 1107 90-91 0.999045212620068 1107 92-93 0.9754122827365315 1107 94-95 1.0172711007998316 1107 96-97 1.0639021270337174 1107 98-99 1.2409213896189115 1107 100-101 1.6881108658561352 1108 1 0.04232598220203698 1108 2 0.08631790983613286 1108 3 0.021156954262316674 1108 4 0.04702390270972501 1108 5 -0.02685298084001886 1108 6 -0.02675559612773526 1108 7 0.0026417371485933927 1108 8 0.0454493499532731 1108 9 0.06187098871016872 1108 10-11 0.02874253597318699 1108 12-13 0.1554078489521089 1108 14-15 0.09684498163215238 1108 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1.0444328292198506 2316 100-101 1.5813112277283317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 29.0 6 78.0 7 224.0 8 578.0 9 1112.0 10 1812.0 11 3003.0 12 4765.0 13 7070.0 14 9002.0 15 12762.0 16 17381.0 17 24876.0 18 22197.0 19 13134.0 20 10627.0 21 10773.0 22 11131.0 23 12029.0 24 13488.0 25 16401.0 26 19818.0 27 24264.0 28 30661.0 29 38141.0 30 48024.0 31 60585.0 32 78537.0 33 103518.0 34 143015.0 35 206457.0 36 318609.0 37 487432.0 38 376988.0 39 29312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54548084452206 20.25983225830978 15.404029750482962 21.7906571466852 2 15.365664897930811 20.988020483351484 40.56412632944829 23.08218828926941 3 19.21417151331186 25.384433579720557 30.615890817248648 24.78550408971893 4 12.78598528996727 17.531687835143085 37.43166220774689 32.25066466714276 5 13.16978396726788 38.48387772543654 34.14579660331956 14.200541703976018 6 31.429246703895082 37.06559770141576 17.25694557082281 14.248210023866347 7 28.048288805987443 33.92520332738606 20.508376393169126 17.518131473457377 8 26.485019811386334 35.962833375813894 19.283268067752623 18.268878745047154 9 26.487661329564148 17.03686539842899 20.73948606251593 35.73598720949093 10-11 24.71884051254721 27.66573209444586 27.082466343814442 20.532961049192483 12-13 25.620851239941366 25.01903627110616 28.194119449067372 21.1659930398851 14-15 22.518849781032046 26.084320040781332 26.871214403225434 24.52561577496119 16-17 21.239822045091177 29.766943948837966 27.428296684199548 21.56493732187131 18-19 21.147531107352226 27.714831892856317 30.55256389461733 20.585073105174132 20-21 21.956252196925128 26.725662240415264 30.869540937940183 20.448544624719425 22-23 21.75813919215985 26.823702026424414 30.71177772149658 20.706381059919163 24-25 21.555877377945638 26.91405797437264 30.937831637972984 20.59223300970874 26-27 21.419602400104733 27.279815647487222 30.642673343150328 20.657908609257714 28-29 21.08018991433464 27.219826537492786 30.97969473750067 20.720288810671907 30-31 21.198002919109975 26.95832123602689 31.02985171115965 20.813824133703484 32-33 21.090233586726043 27.11386961880335 30.87224109002968 20.92365570444093 34-35 21.592835461222975 27.039367889334294 30.670690733832284 20.697105915610443 36-37 21.660137373516427 26.762300903287755 30.550488115543352 21.02707360765247 38-39 21.390898347892577 27.086799360474544 30.716500220126512 20.80580207150636 40-41 21.48767301103206 27.154874083953608 30.56667839904275 20.79077450597158 42-43 21.53104226041527 27.108426056035267 30.323377013762336 21.037154669787128 44-45 21.437379799337304 27.45821071900271 30.04900711356211 21.055402368097877 46-47 21.610098014227123 27.60411984150891 29.606761359686722 21.17902078457724 48-49 21.846560233565818 27.763375582176703 29.19853094515374 21.191533239103737 50-51 21.853164029010358 27.90316750469217 28.927196051625458 21.31647241467201 52-53 22.066153810505828 28.05656092870218 28.79129689274046 21.085988368051535 54-55 21.77827467154807 27.938132863730104 28.810760710892787 21.47283175382904 56-57 21.881849989572956 28.352156081284612 28.73177004889126 21.034223880251176 58-59 22.056167018096716 27.897374701670646 28.70644391408115 21.340014366151493 60-61 21.929165604652777 27.81083022452904 29.084111499872556 21.17589267094562 62-63 21.870681465347452 27.870218041105733 29.27038487383275 20.988715619714068 64-65 21.877632828973283 27.733924971615266 29.0408508469078 21.34759135250365 66-67 21.723196700419397 28.108974210440945 28.778575897305185 21.389253191834463 68-69 21.69214727622402 28.095256852885974 28.89751372894316 21.315082141946846 70-71 21.71796000648794 28.071297819588942 28.866255763839003 21.344486410084112 72-73 21.85886414718354 28.053085246889264 28.544407627963018 21.54364297796418 74-75 21.75728155339806 28.37287114488959 28.3658502676275 21.503997034084854 76-77 21.800797089695763 28.208031142109043 28.57015084459068 21.421020923604512 78-79 21.85647751233867 28.173297495191974 28.40646940241444 21.563755590054917 80-81 21.67161758231573 28.502050652269624 28.456866788701717 21.369464976712933 82-83 21.829858029654588 28.47966373859105 28.42335756275358 21.267120669000786 84-85 21.780435782500344 28.253475404252164 28.302436345562754 21.663652467684734 86-87 21.91197256528489 28.034942187825845 28.52716824617096 21.52591700071831 88-89 21.952220960678453 28.057603633246053 28.46838288110851 21.521792524966983 90-91 21.815794492647292 28.03745724718571 28.59441700792058 21.55233125224641 92-93 21.81806975086593 28.09978617050562 28.40549190515869 21.67665217346976 94-95 21.676645735338415 28.05758046203397 28.399193641819405 21.866580160808212 96-97 21.871283916861692 27.939476794031098 28.307991751048494 21.881247538058716 98-99 21.711905160782198 28.11283868625198 28.126370727414407 22.04888542555141 100-101 21.582836513705082 28.09951255053748 28.500475306098515 21.817175629658916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 478.0 1 362.5 2 324.0 3 431.5 4 402.5 5 408.0 6 764.0 7 1786.5 8 3086.5 9 3761.5 10 3594.0 11 3293.5 12 3356.0 13 3589.5 14 3972.0 15 4265.5 16 4392.0 17 4549.5 18 4650.0 19 4827.0 20 5258.0 21 5921.0 22 7019.0 23 8445.5 24 10091.5 25 12344.0 26 15267.0 27 18451.0 28 20869.5 29 23459.0 30 27812.5 31 31957.0 32 36023.0 33 41761.5 34 48704.0 35 53530.5 36 58658.5 37 63900.5 38 66809.0 39 68226.5 40 68940.0 41 73106.0 42 77529.5 43 81048.5 44 85924.5 45 89808.0 46 87103.0 47 83542.5 48 85090.0 49 82917.0 50 77575.0 51 71749.5 52 65848.0 53 62556.5 54 59800.5 55 54761.0 56 48293.5 57 41943.5 58 37119.0 59 32911.0 60 27327.0 61 21362.5 62 16468.5 63 12686.5 64 9632.0 65 7362.0 66 5352.5 67 4087.5 68 2952.5 69 1858.5 70 1256.0 71 935.0 72 703.0 73 520.5 74 401.5 75 311.5 76 193.0 77 105.5 78 72.5 79 43.0 80 28.0 81 19.0 82 12.0 83 10.0 84 4.0 85 3.5 86 5.0 87 4.0 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03906666357717172 2 0.0 3 0.0 4 1.3902727251662535E-4 5 0.009917278772852608 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0058854878698704725 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.08540908774938E-4 22-23 0.0015988136339411916 24-25 0.0 26-27 0.0012744166647357324 28-29 0.0016219848460272959 30-31 2.3171212086104225E-5 32-33 2.3171212086104225E-5 34-35 0.0 36-37 4.8659545380818877E-4 38-39 0.0 40-41 1.6219848460272956E-4 42-43 0.022082165118057325 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3171212086104225E-4 84-85 3.4756818129156334E-4 86-87 0.0 88-89 0.0 90-91 0.011284380285932757 92-93 0.002595175753643673 94-95 0.0 96-97 0.0 98-99 3.9391060546377176E-4 100-101 0.013578330282457075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2157850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.43723749516487 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08020308467928 35.39803153007491 2 14.495695415708933 14.042628830165327 3 5.026069043674643 7.303466998066946 4 2.397175520141733 4.644502399468021 5 1.2659940590428223 3.0660627452662474 6 0.7884378018783706 2.2913849435849034 7 0.5228789535636748 1.7728768438490898 8 0.35951846926061765 1.3931265183579733 9 0.2810149828689681 1.2250430518431503 >10 1.559593295494807 14.102401179945783 >50 0.13941297770062178 4.705539443757716 >100 0.07979348600607547 6.98668202647765 >500 0.003351178392677033 1.1152900617747545 >1k 5.744877244589176E-4 0.5241603575423418 >5k 9.574795407648628E-5 0.35029049553329566 >10k+ 1.9149590815297255E-4 1.0785125742919093 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12721 0.5895219778946637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10534 0.48817109623004384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7553 0.3500243297726904 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 2904 0.13457839979609335 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.12350256041893551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.3171212086104225E-5 0.0 18-19 0.0 0.0 0.0 4.634242417220845E-5 0.0 20-21 0.0 0.0 0.0 1.1585606043052113E-4 0.0 22-23 0.0 0.0 0.0 3.243969692054592E-4 4.634242417220845E-5 24-25 0.0 0.0 0.0 4.634242417220845E-4 9.26848483444169E-5 26-27 0.0 0.0 0.0 6.487939384109184E-4 9.26848483444169E-5 28-29 0.0 0.0 0.0 0.001251245452649628 9.26848483444169E-5 30-31 0.0 0.0 0.0 0.0024329772690409434 1.1585606043052113E-4 32-33 0.0 0.0 0.0 0.004054962115068239 1.3902727251662535E-4 34-35 0.0 0.0 0.0 0.005839145445698265 1.3902727251662535E-4 36-37 0.0 0.0 0.0 0.008619690896030771 1.3902727251662535E-4 38-39 0.0 0.0 0.0 0.011377065134277175 1.3902727251662535E-4 40-41 0.0 0.0 0.0 0.014852746947192807 1.3902727251662535E-4 42-43 0.0 0.0 0.0 0.020390666635771718 1.3902727251662535E-4 44-45 0.0 0.0 0.0 0.027272516625344672 1.3902727251662535E-4 46-47 0.0 0.0 0.0 0.04353870750978983 1.3902727251662535E-4 48-49 0.0 2.3171212086104225E-5 0.0 0.07203929837569803 1.3902727251662535E-4 50-51 0.0 4.634242417220845E-5 0.0 0.09704103621660448 1.3902727251662535E-4 52-53 0.0 4.634242417220845E-5 0.0 0.12248302708714692 1.3902727251662535E-4 54-55 0.0 4.634242417220845E-5 0.0 0.15388001946381813 1.3902727251662535E-4 56-57 0.0 4.634242417220845E-5 0.0 0.17976226336399656 1.3902727251662535E-4 58-59 0.0 4.634242417220845E-5 0.0 0.21071900271103183 1.3902727251662535E-4 60-61 0.0 4.634242417220845E-5 0.0 0.2594944041522812 1.3902727251662535E-4 62-63 0.0 4.634242417220845E-5 0.0 0.29686956924716734 1.3902727251662535E-4 64-65 0.0 4.634242417220845E-5 0.0 0.3405704752415599 1.3902727251662535E-4 66-67 0.0 4.634242417220845E-5 0.0 0.41689644785318725 1.3902727251662535E-4 68-69 0.0 4.634242417220845E-5 0.0 0.4985517992446185 1.3902727251662535E-4 70-71 0.0 4.634242417220845E-5 0.0 0.572004541557569 1.3902727251662535E-4 72-73 0.0 4.634242417220845E-5 0.0 0.6591514702134069 1.3902727251662535E-4 74-75 0.0 4.634242417220845E-5 0.0 0.7465069397780197 1.3902727251662535E-4 76-77 0.0 4.634242417220845E-5 0.0 0.8208633593623282 1.3902727251662535E-4 78-79 0.0 4.634242417220845E-5 0.0 0.9178812243668466 1.3902727251662535E-4 80-81 0.0 4.634242417220845E-5 0.0 1.0576731468823135 1.621984846027296E-4 82-83 0.0 4.634242417220845E-5 0.0 1.1870380239590332 2.3171212086104225E-4 84-85 0.0 4.634242417220845E-5 0.0 1.3308617373774823 2.3171212086104225E-4 86-87 0.0 4.634242417220845E-5 0.0 1.566906874898626 2.3171212086104225E-4 88-89 0.0 4.634242417220845E-5 0.0 1.872651018374771 2.3171212086104225E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6840 0.0 47.79917 1 GTATCAA 11315 0.0 36.454674 1 ATCAACG 14175 0.0 28.686506 3 TATCAAC 14440 0.0 28.357443 2 TCAACGC 14405 0.0 28.195501 4 CAACGCA 14725 0.0 27.582764 5 AACGCAG 15105 0.0 26.948633 6 TAACGCA 215 1.6625563E-9 24.304192 4 GTACATG 10390 0.0 23.600554 1 ACGCAGA 17435 0.0 23.104195 7 CGCAGAG 17480 0.0 22.854486 8 TACATGG 10670 0.0 22.527502 2 ACCGTGC 360 0.0 22.43176 8 GAGTACT 10945 0.0 21.852474 12-13 ACATGGG 10790 0.0 21.52853 3 TGGTATC 3475 0.0 21.462082 2 ATACCGT 200 5.392649E-7 21.374165 6 GCAGAGT 18500 0.0 21.260601 9 ATATGCG 115 0.0037226635 20.653763 5 GTACTTT 11595 0.0 20.60506 14-15 >>END_MODULE