FastQCFastQC Report
Thu 26 May 2016
SRR937732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749319
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92330.5278053916981408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72280.4131893611170976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.27827971913641825No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25560.14611400207737985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.10135372679311205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.049.1792681
GTATCAA82400.041.737181
TATCAAC104200.032.6669122
ATCAACG104500.032.4357263
TCAACGC104950.032.388174
CAACGCA105650.032.0808225
AACGCAG109050.031.0147656
ACGCAGA124700.027.0073227
CGCAGAG124300.026.9031588
GTACATG84800.025.4666611
GGACCGA2651.8189894E-1225.0955266
GCAGAGT131200.025.0176189
TACATGG86650.024.5794582
ACATGGG86250.023.5352653
AGGACCG3050.023.3785115
GAGTACT74050.022.5438112-13
AGTACTT77200.022.02382912-13
TCGCGGA1306.2937033E-1021.91720682-83
CATGGGG33200.021.4790974
GTGTACG2005.3160693E-721.4054581
Produced by FastQC (version 0.11.5)