##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937732_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749319 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771252698907404 33.0 31.0 34.0 30.0 34.0 2 31.87962287038556 33.0 31.0 34.0 30.0 34.0 3 31.918949602673955 33.0 31.0 34.0 30.0 34.0 4 35.53539691731468 37.0 35.0 37.0 33.0 37.0 5 35.537747546330884 37.0 35.0 37.0 33.0 37.0 6 35.59798069991808 37.0 35.0 37.0 33.0 37.0 7 35.51978798606772 37.0 35.0 37.0 33.0 37.0 8 35.52629337473611 37.0 35.0 37.0 33.0 37.0 9 37.09146587900777 39.0 37.0 39.0 33.0 39.0 10-11 37.01476231607843 39.0 37.0 39.0 33.0 39.0 12-13 36.91072440189582 39.0 37.0 39.0 33.0 39.0 14-15 38.03270501263634 40.0 38.0 41.0 33.0 41.0 16-17 37.94971300260272 40.0 38.0 41.0 33.0 41.0 18-19 37.888983084274514 40.0 38.0 41.0 33.0 41.0 20-21 37.913534352510894 40.0 38.0 41.0 33.0 41.0 22-23 37.91145211365108 40.0 38.0 41.0 33.0 41.0 24-25 37.838149874322525 40.0 38.0 41.0 33.0 41.0 26-27 37.74052102561053 40.0 37.5 41.0 33.0 41.0 28-29 37.585114836116226 40.0 37.0 41.0 32.0 41.0 30-31 37.336089358201676 40.0 37.0 41.0 32.0 41.0 32-33 37.20554370014846 39.5 36.5 41.0 32.0 41.0 34-35 37.175028682590195 40.0 36.5 41.0 31.5 41.0 36-37 37.00977008767411 40.0 36.0 41.0 31.0 41.0 38-39 36.83867979482301 39.5 36.0 41.0 31.0 41.0 40-41 36.602149750845896 39.0 36.0 41.0 30.0 41.0 42-43 36.32998669768064 39.0 35.0 41.0 30.0 41.0 44-45 36.05516746802613 39.0 35.0 41.0 29.5 41.0 46-47 35.64695461491014 39.0 35.0 40.0 27.5 41.0 48-49 35.26671836297439 39.0 35.0 40.0 26.0 41.0 50-51 34.39442548786128 38.0 34.0 39.5 24.5 40.5 52-53 34.50986783999945 38.0 34.0 39.5 24.5 40.5 54-55 34.77802190452399 38.0 34.5 40.0 24.5 41.0 56-57 34.54642921045276 38.0 34.0 40.0 23.5 41.0 58-59 34.26377293106632 38.0 34.0 40.0 23.0 41.0 60-61 33.91742786764449 38.0 33.5 40.0 22.0 41.0 62-63 33.547305837300115 37.5 33.0 40.0 21.0 41.0 64-65 33.10668265765135 37.0 32.5 39.5 19.5 41.0 66-67 32.5914641640547 36.0 32.0 39.0 16.0 40.5 68-69 32.12487173580119 36.0 31.0 39.0 13.0 40.0 70-71 31.652397304322424 35.0 31.0 38.5 8.0 40.0 72-73 31.17164307939261 35.0 30.5 38.0 7.5 40.0 74-75 30.60289461213192 35.0 30.0 37.0 4.0 39.0 76-77 30.04251397258019 34.0 29.0 36.5 2.0 39.0 78-79 29.49860031246445 34.0 29.0 36.0 2.0 38.5 80-81 28.923619991550996 34.0 28.5 35.5 2.0 37.0 82-83 28.362716577136588 34.0 27.5 35.0 2.0 37.0 84-85 27.79704673647288 33.5 26.0 35.0 2.0 36.0 86-87 27.18622904112972 33.0 25.5 35.0 2.0 36.0 88-89 26.616964087167634 32.5 25.0 35.0 2.0 35.5 90-91 26.017223273742523 32.0 23.5 34.5 2.0 35.0 92-93 25.308615238272722 32.0 20.5 34.0 2.0 35.0 94-95 24.50084775847058 31.0 16.0 34.0 2.0 35.0 96-97 23.579156231653577 31.0 3.5 34.0 2.0 35.0 98-99 22.3563195163375 30.5 2.0 34.0 2.0 35.0 100-101 19.180589132113695 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.003416952428068498 1101 2 -0.17108575502801315 1101 3 -0.15239966072175903 1101 4 -0.10635896922797627 1101 5 -0.20733887445359755 1101 6 -0.05500758901852265 1101 7 -0.10405649882397228 1101 8 -0.03732199315700768 1101 9 0.0501551225950152 1101 10-11 0.1327790989439066 1101 12-13 0.08335664709807844 1101 14-15 0.10662838912912775 1101 16-17 0.12857670036763835 1101 18-19 0.2772109586518283 1101 20-21 0.2572443220512852 1101 22-23 0.17118022314274128 1101 24-25 0.07073463023125015 1101 26-27 0.20655658911069708 1101 28-29 0.1774920319011386 1101 30-31 0.14040543043078912 1101 32-33 0.15555675554580262 1101 34-35 0.2560198912559457 1101 36-37 0.31220462607439003 1101 38-39 0.3429918398668619 1101 40-41 0.28574934083569303 1101 42-43 0.1965282453179853 1101 44-45 0.37058943335193106 1101 46-47 0.2086166334168169 1101 48-49 0.29791472442247624 1101 50-51 0.15459838665741188 1101 52-53 0.31850644040911646 1101 54-55 0.3772652449684628 1101 56-57 0.45232362029952355 1101 58-59 0.5662933164793529 1101 60-61 0.5131131918388618 1101 62-63 0.5361505846358341 1101 64-65 0.49933401042799375 1101 66-67 0.5691369294412212 1101 68-69 0.6715895889071604 1101 70-71 0.5087773700192315 1101 72-73 0.7758620298910586 1101 74-75 0.6069479785381588 1101 76-77 0.5913684506636834 1101 78-79 0.7605492605869166 1101 80-81 1.0272573662152915 1101 82-83 0.9897377211183169 1101 84-85 1.0786594647697996 1101 86-87 1.475581985338092 1101 88-89 1.6127132234118342 1101 90-91 1.5811049711094185 1101 92-93 1.498810061712902 1101 94-95 1.8112059664793705 1101 96-97 1.7797627305938981 1101 98-99 1.8950436426866624 1101 100-101 2.5907019233226194 1102 1 0.08823343691853225 1102 2 0.08800048045209508 1102 3 -0.01112313526239106 1102 4 0.0075950926893000315 1102 5 -0.05615809939349958 1102 6 0.07592574060844015 1102 7 0.071475610351456 1102 8 0.12208514498790635 1102 9 0.13894428068773834 1102 10-11 0.2223806954411245 1102 12-13 0.1802561287342499 1102 14-15 0.35580859724394287 1102 16-17 0.3074687037563848 1102 18-19 0.27781426729793424 1102 20-21 0.30750091812345914 1102 22-23 0.3471545916565262 1102 24-25 0.21208016527858575 1102 26-27 0.2893500746056006 1102 28-29 0.21899208701184136 1102 30-31 0.42651061655789846 1102 32-33 0.4404394053071172 1102 34-35 0.5295407668987338 1102 36-37 0.5125894797789101 1102 38-39 0.5621565943591875 1102 40-41 0.6044472543542412 1102 42-43 0.8273177376304446 1102 44-45 0.7118357852982413 1102 46-47 0.586056655680899 1102 48-49 0.8400231790505259 1102 50-51 0.6703060678609134 1102 52-53 0.6837266747314104 1102 54-55 0.7336874840920231 1102 56-57 0.6737332873953363 1102 58-59 0.7233351388755054 1102 60-61 0.7068382162669664 1102 62-63 0.845174145834406 1102 64-65 0.744009443952045 1102 66-67 0.8139851293576399 1102 68-69 0.9543355272807901 1102 70-71 0.9899351666642957 1102 72-73 0.9220322563427068 1102 74-75 0.9491911715584571 1102 76-77 1.0566442643707639 1102 78-79 1.1824645077624538 1102 80-81 1.3853801987566605 1102 82-83 1.4705082075114682 1102 84-85 1.680009418370144 1102 86-87 1.6813145164578565 1102 88-89 1.755585130949978 1102 90-91 1.9653263570999222 1102 92-93 2.167928238773726 1102 94-95 2.311529842859443 1102 96-97 2.174116970546212 1102 98-99 2.436208993214965 1102 100-101 2.907445346321012 1103 1 -0.07450320400711163 1103 2 -0.15661585777978004 1103 3 -0.0694556076919497 1103 4 -0.04681650110185842 1103 5 -0.04163325371050064 1103 6 -0.015372136402845626 1103 7 -0.08444364117879388 1103 8 -0.1246377171727886 1103 9 -0.11777141444901673 1103 10-11 0.020672231896092796 1103 12-13 0.05026649186346077 1103 14-15 0.05524314488205562 1103 16-17 0.11396680546355498 1103 18-19 0.048783946266915734 1103 20-21 -0.0066646078457068825 1103 22-23 -0.049696276134476136 1103 24-25 2.611939725127854E-4 1103 26-27 -0.06355757409401264 1103 28-29 -0.08272299473097178 1103 30-31 -0.05797067815529999 1103 32-33 0.05432615987206191 1103 34-35 -0.03760020394246055 1103 36-37 0.014109082557908437 1103 38-39 0.122685017097389 1103 40-41 0.13676359129475912 1103 42-43 0.02741604208045345 1103 44-45 0.1153148588796924 1103 46-47 0.07905702371726164 1103 48-49 0.1334669663384247 1103 50-51 0.12483097811168875 1103 52-53 0.012390089334061827 1103 54-55 -0.04800302960926217 1103 56-57 0.0693789975118122 1103 58-59 0.0053365464531793805 1103 60-61 0.17984274892534557 1103 62-63 0.2821437214949327 1103 64-65 0.1997487127864659 1103 66-67 0.5351870909268897 1103 68-69 0.7108925470901895 1103 70-71 0.6355879792369308 1103 72-73 0.5354031143194113 1103 74-75 0.45348735043704735 1103 76-77 0.6007151662535613 1103 78-79 0.7068306135503128 1103 80-81 0.7771185894692287 1103 82-83 0.871388912075119 1103 84-85 1.0957776781392496 1103 86-87 1.1084978760487552 1103 88-89 1.2514167962822391 1103 90-91 1.2909428180105884 1103 92-93 1.366693541338357 1103 94-95 1.5406016486912044 1103 96-97 1.6845206160432546 1103 98-99 1.739071928724666 1103 100-101 2.474845072553613 1104 1 0.05916927279179163 1104 2 0.014412351595304074 1104 3 0.03978605174491534 1104 4 0.046955023992126144 1104 5 0.016398071052229568 1104 6 0.12839281614336073 1104 7 0.19603622053480052 1104 8 0.16309758003782093 1104 9 0.32134904720673774 1104 10-11 0.15642426281335986 1104 12-13 0.14529123750586592 1104 14-15 0.3123488906033245 1104 16-17 0.30819479400103234 1104 18-19 0.2341488541197947 1104 20-21 0.15080114918034582 1104 22-23 0.19730023384264683 1104 24-25 0.09733131648479798 1104 26-27 0.18622405300592249 1104 28-29 0.2241050608464974 1104 30-31 0.32186063740586945 1104 32-33 0.28414860249371543 1104 34-35 0.31043312544734647 1104 36-37 0.3152431756777645 1104 38-39 0.486743696803984 1104 40-41 0.5716291498298745 1104 42-43 0.4869602030290068 1104 44-45 0.5055883097301788 1104 46-47 0.5617924195160882 1104 48-49 0.6971404947449784 1104 50-51 0.7062056922058702 1104 52-53 0.6003424449351868 1104 54-55 0.6675127839407011 1104 56-57 0.7070996708036077 1104 58-59 0.9078410157856709 1104 60-61 0.8593840508156134 1104 62-63 0.8662309260589183 1104 64-65 0.879033192372134 1104 66-67 1.0817692017663347 1104 68-69 1.0842029093690186 1104 70-71 1.3003724325324129 1104 72-73 1.278276665072621 1104 74-75 1.2573682755888704 1104 76-77 1.3915400538629967 1104 78-79 1.5187822987515602 1104 80-81 1.6427716049541523 1104 82-83 1.7036220009096716 1104 84-85 1.8670426635481938 1104 86-87 2.0032888033038 1104 88-89 2.0210734792488267 1104 90-91 2.0470584094084394 1104 92-93 2.203228198382817 1104 94-95 2.2772845166881552 1104 96-97 2.47680296612344 1104 98-99 2.876519676969796 1104 100-101 3.2888104220817027 1105 1 -0.0345363569065249 1105 2 0.05627123165970005 1105 3 -0.06416984730232755 1105 4 -0.1265134674648678 1105 5 -0.13216579049408494 1105 6 -0.026853344870040985 1105 7 -0.08357576135072264 1105 8 -0.09235416453156375 1105 9 0.01987949725571525 1105 10-11 -0.04268574607043263 1105 12-13 -0.10632402215316716 1105 14-15 -0.02350003383554622 1105 16-17 -0.026699039715339268 1105 18-19 0.04971655493067573 1105 20-21 0.15460261039132916 1105 22-23 0.035147732872545134 1105 24-25 -0.03178077158368353 1105 26-27 -0.14989199663211394 1105 28-29 -0.14437427683867554 1105 30-31 -0.20067016399196547 1105 32-33 -0.06728364443781487 1105 34-35 0.017114090678262528 1105 36-37 -0.03932007879109278 1105 38-39 -0.012421849660235296 1105 40-41 0.0716725125052804 1105 42-43 -0.03468027369357429 1105 44-45 -0.008142926183900556 1105 46-47 0.05666533908054561 1105 48-49 0.07044198430846649 1105 50-51 0.010493816058556149 1105 52-53 0.037675317957045706 1105 54-55 0.23439109381927636 1105 56-57 0.2572108015576404 1105 58-59 0.33202554731867195 1105 60-61 0.4718026207145769 1105 62-63 0.5539604563744263 1105 64-65 0.502454763190606 1105 66-67 0.7356333164839626 1105 68-69 0.5981423417182441 1105 70-71 0.7165526589300555 1105 72-73 0.6370102414593113 1105 74-75 0.6938655832114726 1105 76-77 0.7647806689013947 1105 78-79 0.8888324215568808 1105 80-81 1.170152354817116 1105 82-83 1.1322120134848497 1105 84-85 1.3486335400412877 1105 86-87 1.51642908378248 1105 88-89 1.4287218212936104 1105 90-91 1.210314434874558 1105 92-93 1.3566111541829073 1105 94-95 1.3660704284884737 1105 96-97 1.3896192495863637 1105 98-99 1.448422219912743 1105 100-101 1.9732769110725847 1106 1 -0.07779924078226585 1106 2 -0.1392067182608585 1106 3 -0.017115261967219908 1106 4 -0.09603179694710207 1106 5 -0.042762325872161 1106 6 0.0751357964314252 1106 7 -0.09330084218117207 1106 8 0.03518435270638065 1106 9 0.04797175006551413 1106 10-11 -0.2525229285961572 1106 12-13 -0.3480313206215726 1106 14-15 -0.521188695790876 1106 16-17 -0.30593790475655425 1106 18-19 -0.030880783745374174 1106 20-21 -0.0024735334813712484 1106 22-23 -0.25825809079027096 1106 24-25 -0.34729281554050573 1106 26-27 -0.15206045114306477 1106 28-29 0.05906374849739393 1106 30-31 0.10297737135707763 1106 32-33 0.2153713746331931 1106 34-35 0.1588061782381942 1106 36-37 0.016853851196877656 1106 38-39 -0.2510903416359227 1106 40-41 0.03645104640321506 1106 42-43 0.1888766532438808 1106 44-45 0.16217953382501804 1106 46-47 0.3680874262647009 1106 48-49 0.4318716206934994 1106 50-51 0.37435213499665565 1106 52-53 0.4331129229680073 1106 54-55 0.5203274838701688 1106 56-57 0.2520786950425773 1106 58-59 0.16538486970420507 1106 60-61 0.41174068351538295 1106 62-63 0.23898439442913144 1106 64-65 0.5714140692851686 1106 66-67 0.7758321923618254 1106 68-69 0.6670655331558564 1106 70-71 0.5575021346374882 1106 72-73 0.47793665328925883 1106 74-75 0.7934983837804914 1106 76-77 1.0553279218360743 1106 78-79 0.9093771610914523 1106 80-81 0.8099457789793725 1106 82-83 0.888434928898473 1106 84-85 0.8889604485659 1106 86-87 1.2650554329911863 1106 88-89 1.4772857967770463 1106 90-91 1.5333688167975161 1106 92-93 1.601800423111996 1106 94-95 1.8584615990511324 1106 96-97 1.8712854921026967 1106 98-99 1.9396241944506727 1106 100-101 2.1381529207922654 1107 1 0.006995667875319356 1107 2 0.16514461541980907 1107 3 0.16547182690772289 1107 4 0.1840977314732939 1107 5 0.1742099379475306 1107 6 0.15012347308553586 1107 7 0.13208099446971744 1107 8 0.17329889481574412 1107 9 0.2014264024910375 1107 10-11 0.227381482460153 1107 12-13 0.27553594426910166 1107 14-15 0.23961223750965388 1107 16-17 0.24269874033763728 1107 18-19 0.28597523841887806 1107 20-21 0.2803833828854394 1107 22-23 0.3053661514525672 1107 24-25 0.39342807914842837 1107 26-27 0.34145073433942486 1107 28-29 0.25181607777345505 1107 30-31 0.3177309652375939 1107 32-33 0.40346597022634967 1107 34-35 0.44280294563983347 1107 36-37 0.3273240651788001 1107 38-39 0.42140902465882135 1107 40-41 0.5692972061493293 1107 42-43 0.6255253017914129 1107 44-45 0.6607943850703109 1107 46-47 0.6262485445417028 1107 48-49 0.7072229880470644 1107 50-51 0.4850434910698027 1107 52-53 0.6485862750771005 1107 54-55 0.7075552361386457 1107 56-57 0.8588551088600695 1107 58-59 0.7614535352939598 1107 60-61 0.8756466089168171 1107 62-63 1.037531245124427 1107 64-65 0.8167135429590431 1107 66-67 1.0946743310025013 1107 68-69 1.086105593617411 1107 70-71 1.1567763901156454 1107 72-73 1.3497526935620492 1107 74-75 1.2349774565410776 1107 76-77 1.4488152790653182 1107 78-79 1.4605697897195888 1107 80-81 1.737125678316108 1107 82-83 1.8873151880675572 1107 84-85 1.8562123845237863 1107 86-87 2.002135445412744 1107 88-89 1.748971144108328 1107 90-91 1.843450973995708 1107 92-93 2.0079906478692457 1107 94-95 2.4678117682106873 1107 96-97 2.2450841412582108 1107 98-99 2.6491167485307514 1107 100-101 3.310664329825393 1108 1 -0.042531550515352734 1108 2 -0.016001137889762873 1108 3 0.030615176746930928 1108 4 0.029164047497999945 1108 5 0.02153490229349586 1108 6 0.050228876673863 1108 7 0.16859988511373558 1108 8 0.07087279002021774 1108 9 0.21109758933160805 1108 10-11 0.19419721512058885 1108 12-13 0.05023877136789423 1108 14-15 0.10228462128996796 1108 16-17 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2316 60-61 -0.39654530545146116 2316 62-63 -0.6588050561534899 2316 64-65 -0.8264522804915373 2316 66-67 -0.8290081942884768 2316 68-69 -1.0855396790542429 2316 70-71 -0.9589459405497642 2316 72-73 -1.1036245904385602 2316 74-75 -1.3983858423227282 2316 76-77 -1.3265612523126649 2316 78-79 -1.263474082457801 2316 80-81 -1.3810231451058606 2316 82-83 -1.4376332066676056 2316 84-85 -1.781731569297758 2316 86-87 -1.9596604288068527 2316 88-89 -1.8480957182695619 2316 90-91 -2.026670733005222 2316 92-93 -2.1812309488790724 2316 94-95 -2.1442811388712286 2316 96-97 -2.1863534779895666 2316 98-99 -2.3978014048590204 2316 100-101 -2.57273087847722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6965.0 3 1333.0 4 1282.0 5 1366.0 6 1702.0 7 1966.0 8 2358.0 9 2984.0 10 3735.0 11 5010.0 12 6675.0 13 8773.0 14 9764.0 15 12546.0 16 15769.0 17 21259.0 18 18354.0 19 12672.0 20 12432.0 21 13218.0 22 14595.0 23 16116.0 24 18342.0 25 20564.0 26 24103.0 27 27952.0 28 33306.0 29 39739.0 30 48289.0 31 59298.0 32 75258.0 33 96773.0 34 129485.0 35 178961.0 36 253262.0 37 323946.0 38 212819.0 39 16348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15230505415473 19.426858842002662 14.942793166733695 22.478042937108913 2 14.660445071853573 20.125020570138293 41.80975933368234 23.4047750243258 3 18.848795290223844 25.110568153734032 30.371221143761822 25.669415412280305 4 12.082997430125443 16.71611085436575 38.084084552427846 33.116807163080956 5 13.01024737737779 37.93937809336397 34.54023265307995 14.510141876178286 6 31.098806783219107 36.528155126202755 17.925924992236585 14.447113098341552 7 27.904630577543227 33.434810480828666 20.955183727297257 17.70537521433085 8 26.027781040256443 35.455459527281164 19.895556122112826 18.621203310349568 9 26.295187988103606 16.977282610377088 20.825620214930304 35.901909186588995 10-11 24.31215577710083 27.226359087828296 27.644052968431037 20.817432166639836 12-13 25.296577771398994 24.70747581384676 28.402855901203672 21.593090513550578 14-15 22.29258948503405 25.717417129323145 27.04154899136066 24.94844439428215 16-17 21.090780454689977 29.425522102905326 27.543287780314333 21.94040966209036 18-19 20.96185374136021 27.486793278577903 30.566900879989827 20.984452100072062 20-21 21.763931222171497 26.618499808390382 30.69431420655001 20.92325476288811 22-23 21.569669853745722 26.626957074685347 30.571926970250747 21.23144610131818 24-25 21.2729275678475 26.714447590120567 30.809672973168055 21.202951868863877 26-27 21.115321353049794 27.050740799516927 30.51454643682336 21.319391410609924 28-29 20.78045001810523 27.004867785982427 30.89353895374687 21.321143242165476 30-31 20.94098240238172 26.768972315823188 30.904925419399493 21.385119862395598 32-33 20.806549060804155 26.95099176411372 30.671707872835785 21.57075130224634 34-35 21.311561351868168 26.907705419361307 30.495785259509127 21.2849479692614 36-37 21.35681364249462 26.598808607562596 30.455037209173096 21.58934054076969 38-39 21.063612673331527 26.87370745095366 30.579637632326524 21.483042243388294 40-41 21.18832886385011 26.93196801217013 30.512585385076243 21.36711773890352 42-43 21.20188910596276 26.796628388660437 30.338802315526753 21.66268018985005 44-45 21.097168357555727 27.176320296970147 30.0262942318819 21.700217113592227 46-47 21.303706555455967 27.227744468611135 29.632478199133722 21.836070776799176 48-49 21.514999162057986 27.35079976910042 29.328658046606765 21.805543022234826 50-51 21.496373637643355 27.53018913002252 29.06395219927891 21.909485033055216 52-53 21.62345526385682 27.68166595565279 29.02088375252591 21.673995027964477 54-55 21.319685425521737 27.54337086479336 29.105701676741326 22.03124203294358 56-57 21.46765860703188 27.89684797671027 28.986191624253188 21.649301792004664 58-59 21.610862464669044 27.467812756249625 29.03230978547182 21.889014993609507 60-61 21.470376494319396 27.44983044382424 29.36342981703053 21.716363244825832 62-63 21.41252395258619 27.478212166367534 29.48876905755378 21.62049482349249 64-65 21.444963783409495 27.39308130534363 29.29471719444704 21.867237716799835 66-67 21.330183276059564 27.690721649484534 29.044616265750285 21.934478808705613 68-69 21.194649921996163 27.642480132503433 29.24880359756894 21.914066347931467 70-71 21.2569736894103 27.617986434344942 29.209542578665737 21.915497297579016 72-73 21.37253773217182 27.636111189870462 28.918599779072867 22.072751298884853 74-75 21.227571159218098 27.873585357980723 28.798191916151282 22.100651566649894 76-77 21.35161238765588 27.751210013971946 28.843874542360084 22.053303056012087 78-79 21.395822151293643 27.76490192360679 28.74918873526334 22.090087189836236 80-81 21.18087039902119 28.015886154003017 28.788685053302306 22.014558393673493 82-83 21.389159187245454 27.84443758437079 28.85711618412921 21.909287044254537 84-85 21.319728346976405 27.720875573305342 28.716165428066553 22.2432306516517 86-87 21.476438186303778 27.50384093860655 28.93036482115473 22.08935605393494 88-89 21.579417362948437 27.50333510709288 28.81303409685185 22.104213433106832 90-91 21.30207712822017 27.45948729345565 29.025953792717907 22.212481785606276 92-93 21.42198688696414 27.414864781661798 28.84824932809652 22.314899003277546 94-95 21.20058557606561 27.410274958432918 28.781602006445688 22.60753745905578 96-97 21.297792477158076 27.402604990368328 28.688087945776243 22.611514586697353 98-99 21.23197159790971 27.576617307838813 28.469853424272262 22.721557669979212 100-101 21.40241466840186 26.86564129876117 29.211045110747463 22.520898922089504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2353.0 1 1380.5 2 426.0 3 416.5 4 368.0 5 374.0 6 601.5 7 1301.0 8 2300.0 9 2845.5 10 2619.0 11 2361.5 12 2456.5 13 2638.0 14 2798.5 15 3004.5 16 3172.5 17 3245.5 18 3392.0 19 3599.0 20 3867.0 21 4477.0 22 5469.0 23 6656.0 24 8050.5 25 9866.0 26 12366.5 27 15065.5 28 16870.0 29 18847.5 30 22490.0 31 25899.5 32 29206.5 33 33721.0 34 39197.5 35 43370.5 36 47647.0 37 51576.0 38 53495.5 39 54745.5 40 56094.5 41 59552.0 42 62719.5 43 65641.5 44 68991.5 45 71239.0 46 70107.0 47 68181.0 48 69342.0 49 67498.5 50 62712.0 51 57905.5 52 53191.0 53 50923.5 54 49191.5 55 44914.0 56 39440.5 57 34592.0 58 30612.5 59 26833.0 60 22536.5 61 18184.5 62 14056.0 63 10640.0 64 8129.5 65 6284.5 66 4601.5 67 3446.5 68 2491.0 69 1628.5 70 1155.0 71 835.5 72 615.5 73 483.5 74 363.0 75 244.5 76 174.5 77 127.5 78 85.0 79 54.5 80 35.0 81 23.5 82 15.5 83 12.5 84 10.5 85 7.0 86 5.5 87 5.5 88 4.5 89 4.0 90 3.5 91 1.5 92 2.0 93 3.0 94 1.0 95 1.5 96 2.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.357739211658937 2 0.30217473199570805 3 0.307090930813648 4 0.3009742648424901 5 0.3008599346374218 6 0.22597365031763791 7 0.21551243655388183 8 0.22202925824277905 9 0.22385854152387302 10-11 0.2230582300883944 12-13 0.22583073756130245 14-15 0.2214290246661701 16-17 0.2134544928626511 18-19 0.20676617586615134 20-21 0.20476539727745485 22-23 0.2131686673499802 24-25 0.2122540257094332 26-27 0.21951399373127486 28-29 0.2267739617531165 30-31 0.2301467028026335 32-33 0.22691687450945197 34-35 0.22617372817650755 36-37 0.2123397733632345 38-39 0.19982061590824773 40-41 0.19464717412890387 42-43 0.20725207923769193 44-45 0.21088206324861278 46-47 0.21742746748877706 48-49 0.22703120471452035 50-51 0.22328689049853112 52-53 0.23772107888841315 54-55 0.2200856447566167 56-57 0.18941656724702585 58-59 0.17006618003920382 60-61 0.18807318733747247 62-63 0.18069888911056245 64-65 0.1884161779526776 66-67 0.18973097531096386 68-69 0.1858437483386392 70-71 0.18818751754254084 72-73 0.19976345080571353 74-75 0.20599444698193983 76-77 0.21025324712073667 78-79 0.20510838789265995 80-81 0.20133549112540364 82-83 0.18898782897801944 84-85 0.18261392004545768 86-87 0.19015971357997025 88-89 0.19996352866458317 90-91 0.1970481084353397 92-93 0.19130301563065397 94-95 0.1912744330793869 96-97 0.20099250051019854 98-99 0.2019071421507455 100-101 0.17815504204779117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.23445690466326 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6073433908174 40.781503203192095 2 13.015133171450156 13.85707091848033 3 4.3789598648604855 6.993346506394967 4 1.9020960552529054 4.050282019275631 5 1.010059472256614 2.688498372349582 6 0.6454113817171847 2.0614874631481586 7 0.4324807351459024 1.611601394005514 8 0.2998557599065101 1.2770126822686532 9 0.22261932886321936 1.0665917161662697 >10 1.3029583941180112 13.114223630136948 >50 0.11948246721917438 4.433555864849257 >100 0.061129277455751516 5.795881070057619 >500 0.001503904918040279 0.5100365924626707 >1k 7.519524590201395E-4 0.8175796796096875 >5k 2.1484355972003986E-4 0.9413288876026487 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9233 0.5278053916981408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7228 0.4131893611170976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4868 0.27827971913641825 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2556 0.14611400207737985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1773 0.10135372679311205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.858255126709308E-5 0.0 20-21 0.0 0.0 0.0 5.716510253418616E-5 0.0 22-23 0.0 0.0 0.0 1.1433020506837233E-4 0.0 24-25 0.0 0.0 0.0 2.2866041013674463E-4 0.0 26-27 0.0 0.0 0.0 3.4299061520511693E-4 0.0 28-29 0.0 0.0 0.0 9.146416405469786E-4 0.0 30-31 0.0 0.0 0.0 0.0018292832810939573 0.0 32-33 0.0 0.0 0.0 0.003658566562187914 0.0 34-35 0.0 0.0 0.0 0.005173441779343847 0.0 36-37 0.0 0.0 0.0 0.006974142509170712 0.0 38-39 0.0 0.0 0.0 0.009146416405469785 0.0 40-41 0.0 0.0 0.0 0.012147584288514559 0.0 42-43 0.0 0.0 0.0 0.01680654014505073 0.0 44-45 0.0 0.0 0.0 0.023980760513091094 0.0 46-47 0.0 0.0 0.0 0.04041572749166962 0.0 48-49 0.0 0.0 0.0 0.07182795133420491 0.0 50-51 0.0 0.0 0.0 0.09878129717907369 0.0 52-53 0.0 0.0 0.0 0.12570606047267535 0.0 54-55 0.0 0.0 0.0 0.15971929648051614 0.0 56-57 0.0 0.0 0.0 0.18775877927353446 0.0 58-59 0.0 0.0 0.0 0.21997131455154834 0.0 60-61 0.0 0.0 0.0 0.2689046423208117 0.0 62-63 0.0 0.0 0.0 0.30686227040351133 0.0 64-65 0.0 0.0 0.0 0.35062215639343086 0.0 66-67 0.0 0.0 0.0 0.4312821160691675 0.0 68-69 0.0 0.0 0.0 0.5181159068185963 0.0 70-71 0.0 0.0 0.0 0.5951458824834122 0.0 72-73 2.858255126709308E-5 0.0 0.0 0.6930125380219387 0.0 74-75 5.716510253418616E-5 0.0 0.0 0.7894500659971109 0.0 76-77 5.716510253418616E-5 0.0 0.0 0.8699671129165121 0.0 78-79 5.716510253418616E-5 0.0 0.0 0.974322007592669 0.0 80-81 5.716510253418616E-5 0.0 0.0 1.1225225359125466 0.0 82-83 5.716510253418616E-5 0.0 0.0 1.2664928466448944 0.0 84-85 5.716510253418616E-5 0.0 0.0 1.4232681403449 0.0 86-87 5.716510253418616E-5 0.0 0.0 1.6922299477682459 0.0 88-89 5.716510253418616E-5 0.0 0.0 2.0490259352353686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4575 0.0 49.179268 1 GTATCAA 8240 0.0 41.73718 1 TATCAAC 10420 0.0 32.666912 2 ATCAACG 10450 0.0 32.435726 3 TCAACGC 10495 0.0 32.38817 4 CAACGCA 10565 0.0 32.080822 5 AACGCAG 10905 0.0 31.014765 6 ACGCAGA 12470 0.0 27.007322 7 CGCAGAG 12430 0.0 26.903158 8 GTACATG 8480 0.0 25.466661 1 GGACCGA 265 1.8189894E-12 25.095526 6 GCAGAGT 13120 0.0 25.017618 9 TACATGG 8665 0.0 24.579458 2 ACATGGG 8625 0.0 23.535265 3 AGGACCG 305 0.0 23.378511 5 GAGTACT 7405 0.0 22.54381 12-13 AGTACTT 7720 0.0 22.023829 12-13 TCGCGGA 130 6.2937033E-10 21.917206 82-83 CATGGGG 3320 0.0 21.479097 4 GTGTACG 200 5.3160693E-7 21.405458 1 >>END_MODULE