FastQCFastQC Report
Thu 26 May 2016
SRR937732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749319
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107500.6145248522425012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90360.5165438664989062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.3571675606335951No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA25540.14599967187231144No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.11810310183562861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.049.5494651
GTATCAA90900.038.431681
ATCAACG119300.029.3067253
TATCAAC120250.029.1937082
TCAACGC121100.028.871124
CAACGCA123150.028.3905185
AACGCAG126700.027.6293816
CGCAGAG144600.023.947738
ACGCAGA144700.023.931187
CGTAATA1000.001653184823.7519862
GTACATG86050.023.5301461
TACATGG88250.022.6619512
CCTATAC3850.022.209653
GCAGAGT153600.021.9878869
GAGTACT91900.021.88986212-13
ACATGGG86000.021.7634493
AGTACTT94900.021.34803612-13
CGACGTT905.1731913E-621.11136842-43
GTACTTT97350.020.56562414-15
AGAGTAC142600.020.45285610-11
Produced by FastQC (version 0.11.5)