##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937732_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749319 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27672997320671 33.0 31.0 34.0 31.0 34.0 2 32.51783808442028 34.0 31.0 34.0 31.0 34.0 3 32.611041782545094 34.0 31.0 34.0 31.0 34.0 4 36.21353566730825 37.0 35.0 37.0 35.0 37.0 5 36.055808003000024 37.0 35.0 37.0 35.0 37.0 6 36.158398210960954 37.0 35.0 37.0 35.0 37.0 7 36.12201605310409 37.0 35.0 37.0 35.0 37.0 8 36.11739825612138 37.0 35.0 37.0 35.0 37.0 9 37.804854917828024 39.0 38.0 39.0 35.0 39.0 10-11 37.74966858531806 39.0 37.0 39.0 35.0 39.0 12-13 37.67175312221499 39.0 37.0 39.0 35.0 39.0 14-15 38.90615691020334 40.0 38.0 41.0 35.5 41.0 16-17 38.87643591591928 40.0 38.0 41.0 35.5 41.0 18-19 38.87356422699347 40.0 38.0 41.0 35.0 41.0 20-21 38.80356355816178 40.0 38.0 41.0 35.0 41.0 22-23 38.66082972859724 40.0 38.0 41.0 34.5 41.0 24-25 38.59728500062024 40.0 38.0 41.0 34.0 41.0 26-27 38.53248607029364 40.0 38.0 41.0 34.0 41.0 28-29 38.42021695299714 40.0 38.0 41.0 34.0 41.0 30-31 38.48225309391826 40.0 38.0 41.0 34.0 41.0 32-33 38.403357535132244 40.0 38.0 41.0 34.0 41.0 34-35 38.269650646908886 40.0 38.0 41.0 34.0 41.0 36-37 38.11432591768568 40.0 38.0 41.0 34.0 41.0 38-39 37.98684916816201 40.0 37.5 41.0 33.5 41.0 40-41 37.79986812010846 40.0 37.0 41.0 33.0 41.0 42-43 37.586250992529095 40.0 37.0 41.0 33.0 41.0 44-45 37.26106387685722 40.0 37.0 41.0 32.5 41.0 46-47 36.8698879392495 40.0 37.0 41.0 31.0 41.0 48-49 36.50519259208869 40.0 36.0 41.0 30.5 41.0 50-51 36.24777956450482 39.0 36.0 41.0 30.0 41.0 52-53 36.02897670464907 39.0 35.5 41.0 29.5 41.0 54-55 35.81543446335402 39.0 35.0 40.0 29.0 41.0 56-57 35.5543614400804 39.0 35.0 40.0 28.0 41.0 58-59 35.304957815012585 38.0 35.0 40.0 28.0 41.0 60-61 34.97659003303571 38.0 34.5 40.0 27.0 41.0 62-63 34.62280321656599 38.0 34.0 40.0 26.0 41.0 64-65 34.2242832782357 37.0 34.0 40.0 26.0 41.0 66-67 33.810035219419674 36.5 33.5 39.0 25.5 41.0 68-69 33.35492811774182 36.0 33.0 39.0 24.5 40.0 70-71 32.95452916249123 35.5 32.5 38.5 23.5 40.0 72-73 32.51528680589418 35.0 32.0 38.0 22.5 40.0 74-75 32.01122979856733 35.0 31.5 37.0 21.5 39.0 76-77 30.77159826195222 34.0 30.5 36.0 20.0 38.5 78-79 31.34063655628276 34.5 31.0 36.0 20.0 39.0 80-81 31.126964264379453 34.5 31.0 36.0 19.5 37.5 82-83 30.771006031489968 34.0 31.0 35.5 18.0 37.0 84-85 30.37323810008352 34.0 31.0 35.0 16.5 37.0 86-87 29.93229050847787 34.0 30.0 35.0 9.0 36.0 88-89 29.5028265284948 34.0 29.5 35.0 6.5 36.0 90-91 28.996174225512902 34.0 29.0 35.0 2.0 35.0 92-93 28.540749285864955 34.0 29.0 35.0 2.0 35.0 94-95 28.06942358712162 33.5 29.0 35.0 2.0 35.0 96-97 27.531756643585304 33.0 27.0 35.0 2.0 35.0 98-99 26.729247781565284 33.0 25.5 34.5 2.0 35.0 100-101 24.315986678244506 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0728035408722647 1101 2 0.07439596842792895 1101 3 0.010570057572444114 1101 4 0.04416272038051261 1101 5 -0.07654184700942324 1101 6 -0.09103969927546274 1101 7 -0.1452472572121124 1101 8 -0.0951948188347771 1101 9 0.059554223430311026 1101 10-11 0.0034313669148815507 1101 12-13 0.007497752829692672 1101 14-15 -0.010897627318676939 1101 16-17 -0.19375926027212387 1101 18-19 -0.12158365567692897 1101 20-21 -0.027184884685325983 1101 22-23 -0.054437107550711517 1101 24-25 -0.05872340783862029 1101 26-27 -0.12407698149892354 1101 28-29 -0.20213746059197746 1101 30-31 -0.21417130407500196 1101 32-33 -0.10197760697826652 1101 34-35 -0.14746227008155444 1101 36-37 0.04175430905893052 1101 38-39 -0.011737300695557451 1101 40-41 -0.15241476608014182 1101 42-43 -0.08658116795238868 1101 44-45 0.028452677750934185 1101 46-47 0.03741637629686778 1101 48-49 0.05157622706747844 1101 50-51 0.28487554580329544 1101 52-53 0.2771898575837639 1101 54-55 0.313781613121634 1101 56-57 0.28064737996290035 1101 58-59 0.30350967823032704 1101 60-61 0.23536976282872502 1101 62-63 0.37645777014498094 1101 64-65 0.2833919305560926 1101 66-67 0.26830478592069795 1101 68-69 0.32577315734801005 1101 70-71 0.3303485451749637 1101 72-73 0.2906589878891239 1101 74-75 0.3887449471758124 1101 76-77 0.2267197866978634 1101 78-79 0.37859728152212213 1101 80-81 0.41159904194416796 1101 82-83 0.4417000622810541 1101 84-85 0.5124735246146344 1101 86-87 0.7276454402692139 1101 88-89 0.7380784508248333 1101 90-91 0.7785377427159794 1101 92-93 1.0322589233632442 1101 94-95 0.9260675078291136 1101 96-97 1.0736913898169362 1101 98-99 1.363363220554124 1101 100-101 1.943176155806011 1102 1 0.05956468979947971 1102 2 0.14176661908652477 1102 3 0.039102693935873845 1102 4 0.006429990797578 1102 5 0.0016936925728288088 1102 6 -0.05999815227120564 1102 7 -0.058662688790313666 1102 8 0.035882832951571686 1102 9 0.0707772511055822 1102 10-11 0.01746205048474536 1102 12-13 0.02457520162764837 1102 14-15 0.08289302894473138 1102 16-17 0.05145152280730514 1102 18-19 0.0717565635311459 1102 20-21 0.10882630618097267 1102 22-23 0.07497615256888679 1102 24-25 0.011468151141301064 1102 26-27 -0.030471374364843484 1102 28-29 -0.16310704368678586 1102 30-31 0.04412762412690796 1102 32-33 0.08186676363328615 1102 34-35 0.11406524017856867 1102 36-37 0.11101431940875983 1102 38-39 0.0026777840569423006 1102 40-41 0.05621948084100836 1102 42-43 0.12416037643370714 1102 44-45 0.20323909550039332 1102 46-47 0.24888607688575348 1102 48-49 0.2189219728607199 1102 50-51 0.2635518255432743 1102 52-53 0.11827718040932922 1102 54-55 0.09630912887666909 1102 56-57 0.25005253670840943 1102 58-59 0.28778850217764784 1102 60-61 0.31023425676541905 1102 62-63 0.38505811024749903 1102 64-65 0.4010693587319807 1102 66-67 0.38529502728616905 1102 68-69 0.35959141098200575 1102 70-71 0.423597064054654 1102 72-73 0.39724857930388424 1102 74-75 0.3293030456529813 1102 76-77 0.29189764557761677 1102 78-79 0.47639025146529335 1102 80-81 0.46691798421950637 1102 82-83 0.4089683460239151 1102 84-85 0.39737870350138493 1102 86-87 0.511446805139876 1102 88-89 0.5914114199857892 1102 90-91 0.7366458521344796 1102 92-93 0.93585454915738 1102 94-95 0.8773931222164641 1102 96-97 1.1197043810738911 1102 98-99 1.3007910796763014 1102 100-101 1.73361819858032 1103 1 0.09820269697941342 1103 2 0.08057637656992966 1103 3 0.08322412010907243 1103 4 0.005150257333383479 1103 5 -0.08175887593358766 1103 6 0.01815049567941429 1103 7 0.04428547619328782 1103 8 0.06969181562158155 1103 9 0.04803550932506084 1103 10-11 0.10412541343317372 1103 12-13 0.08792168526617417 1103 14-15 0.08862939222890986 1103 16-17 0.04028662314564002 1103 18-19 -0.004967073442955439 1103 20-21 -0.032988113096379834 1103 22-23 0.09681717377294774 1103 24-25 0.1456451701758823 1103 26-27 0.059668802292613066 1103 28-29 -0.05867559530227595 1103 30-31 -0.014906861526135629 1103 32-33 -0.015241245354545185 1103 34-35 0.06370107788098522 1103 36-37 0.02525151561733452 1103 38-39 -0.0550259595831335 1103 40-41 0.050849539726826265 1103 42-43 0.031185674091183557 1103 44-45 0.042255469890221775 1103 46-47 0.08760366385855889 1103 48-49 0.12335212988956812 1103 50-51 0.024433918314265668 1103 52-53 0.08940392051108148 1103 54-55 0.08205659781956598 1103 56-57 0.07166547221810049 1103 58-59 -0.06891379514407703 1103 60-61 -0.17992335319351582 1103 62-63 0.014849685443145688 1103 64-65 -0.021564393431042106 1103 66-67 -0.06272968280448055 1103 68-69 -0.12010944647815336 1103 70-71 0.03334018112896331 1103 72-73 0.08796663762150558 1103 74-75 0.2649554385350221 1103 76-77 0.09416300625325746 1103 78-79 0.07619444966256239 1103 80-81 0.17313053490521568 1103 82-83 0.11875859757999763 1103 84-85 0.20443741498669965 1103 86-87 0.3965069500299805 1103 88-89 0.38143246908313344 1103 90-91 0.5554878612256253 1103 92-93 0.6148730865679575 1103 94-95 0.4682571037242518 1103 96-97 0.5770865771533344 1103 98-99 0.7962085426789614 1103 100-101 1.5224402239425423 1104 1 0.06210429279401808 1104 2 0.07880638945212581 1104 3 0.05455433805475707 1104 4 0.042841189846463124 1104 5 -0.11585462480968545 1104 6 -0.05167658058842761 1104 7 -0.018644039804300405 1104 8 -0.0623414677603904 1104 9 0.015819837742128584 1104 10-11 -0.0780802350143901 1104 12-13 0.011249300482937485 1104 14-15 0.011715630628359008 1104 16-17 0.016648619359848738 1104 18-19 -3.2718333594772275E-4 1104 20-21 0.0065717233786273255 1104 22-23 0.01096031071855208 1104 24-25 -0.0855073918032403 1104 26-27 -0.01566343999778752 1104 28-29 -0.023865903423320844 1104 30-31 -0.008458699210486031 1104 32-33 -0.041116708719172834 1104 34-35 0.0817203748812787 1104 36-37 0.10502778609082242 1104 38-39 -0.19372791425884373 1104 40-41 -0.1509472305231938 1104 42-43 0.07052007217726697 1104 44-45 0.06488146563121688 1104 46-47 0.3161201412358565 1104 48-49 0.31664040840785646 1104 50-51 0.208957375737306 1104 52-53 0.30487389054026437 1104 54-55 0.29192544262856757 1104 56-57 0.2818465130467729 1104 58-59 0.2380430823699271 1104 60-61 0.26624715184104986 1104 62-63 0.3664231533822573 1104 64-65 0.3029122674517808 1104 66-67 0.3150592135332957 1104 68-69 0.27512709951653136 1104 70-71 0.3953250576965175 1104 72-73 0.4459933616007916 1104 74-75 0.32381063216228867 1104 76-77 0.04925968843113537 1104 78-79 0.3091266932674728 1104 80-81 0.4015126036803558 1104 82-83 0.41889119873150804 1104 84-85 0.4281730211070567 1104 86-87 0.6191340959668352 1104 88-89 0.5733252313935147 1104 90-91 0.5858272347303703 1104 92-93 0.8380437822064124 1104 94-95 0.9183257868485768 1104 96-97 1.1709256351757844 1104 98-99 1.431959094772978 1104 100-101 2.1456045113469138 1105 1 0.05261342619650833 1105 2 0.05802176963715766 1105 3 -0.04288103766059237 1105 4 0.002136424066065956 1105 5 -0.0782352801642503 1105 6 -0.13001014185377358 1105 7 -0.08013398133140015 1105 8 -0.086772999763717 1105 9 -0.0037378407314179185 1105 10-11 -0.06038603972002221 1105 12-13 -0.12945479817938121 1105 14-15 0.03671365707069896 1105 16-17 -0.03665661718354585 1105 18-19 0.005650472655716499 1105 20-21 -0.061900998718883216 1105 22-23 -0.007332623055518184 1105 24-25 -0.19725189447226654 1105 26-27 -0.17026360833391863 1105 28-29 -0.18021058554710834 1105 30-31 -0.06575191206230357 1105 32-33 -0.11842707762434657 1105 34-35 -0.12168670835615814 1105 36-37 -0.1421647586422239 1105 38-39 -0.09693769732315616 1105 40-41 -0.25610477601786386 1105 42-43 -0.15444104039780626 1105 44-45 -0.04600495640325164 1105 46-47 -0.14745910294159614 1105 48-49 -0.22872973650131456 1105 50-51 -0.21953924030621863 1105 52-53 -0.19702016888438578 1105 54-55 -0.1539318096677107 1105 56-57 -0.14303209925866867 1105 58-59 -0.09998592837179388 1105 60-61 -0.1988903689924868 1105 62-63 -0.20523422366510147 1105 64-65 -0.1835494049465396 1105 66-67 -0.03851587227476472 1105 68-69 -0.17365046218911573 1105 70-71 -0.16609733812465777 1105 72-73 -0.13215229473777867 1105 74-75 -0.07261039050750284 1105 76-77 -0.3274037983060012 1105 78-79 0.020269010680035393 1105 80-81 0.10187151320913657 1105 82-83 0.10704240326879955 1105 84-85 0.08779797843929771 1105 86-87 0.13406058511337804 1105 88-89 0.16812326695313118 1105 90-91 0.18645199081532127 1105 92-93 0.3164019156544917 1105 94-95 0.4021634614528402 1105 96-97 0.4879284737632297 1105 98-99 0.7687931302480919 1105 100-101 1.3655925365773207 1106 1 -0.1946214399058661 1106 2 -0.013068976257635256 1106 3 -0.1688833881509737 1106 4 -0.048792217647061875 1106 5 -0.15144970289564696 1106 6 -0.13227467710314755 1106 7 -0.1983195899668715 1106 8 -0.11482159996636199 1106 9 -0.09675727131691048 1106 10-11 -0.11884858139531929 1106 12-13 -0.2426781854461879 1106 14-15 -0.18937624614877535 1106 16-17 -0.10763170617072859 1106 18-19 -0.08362563435977677 1106 20-21 -0.10532195453546933 1106 22-23 -0.15380073336341127 1106 24-25 -0.16202829074254055 1106 26-27 -0.2652499938958002 1106 28-29 -0.1793194198222352 1106 30-31 -0.16554402214607222 1106 32-33 -0.15078457267574663 1106 34-35 -0.37736610380805047 1106 36-37 -0.28115859754925765 1106 38-39 -0.34098378547577113 1106 40-41 -0.2926981490570668 1106 42-43 -0.2693564162186277 1106 44-45 -0.2162779134556132 1106 46-47 -0.10464197068836967 1106 48-49 -0.12019236660689359 1106 50-51 -0.02904038776225093 1106 52-53 -0.040827540279643415 1106 54-55 -0.14536644810149113 1106 56-57 -0.19717550797375338 1106 58-59 -0.16519268534535314 1106 60-61 -0.0989236484801026 1106 62-63 -0.11696943955087136 1106 64-65 -0.1493958526256236 1106 66-67 0.012121377034951308 1106 68-69 -0.39322420123068014 1106 70-71 -0.030723740436535252 1106 72-73 0.1770559977274857 1106 74-75 0.10977076470699032 1106 76-77 0.007078003619621853 1106 78-79 0.22484740412458137 1106 80-81 0.12242952853660327 1106 82-83 0.06812981671457052 1106 84-85 0.0863438290902252 1106 86-87 0.1971977809823997 1106 88-89 0.25577725208739466 1106 90-91 0.315590353604037 1106 92-93 0.5660367750964674 1106 94-95 0.4340882556611696 1106 96-97 0.8637211679818897 1106 98-99 1.0039507375361865 1106 100-101 1.5723779743375879 1107 1 0.11606811560250208 1107 2 0.09956991276116156 1107 3 0.06433043756745604 1107 4 0.07835898957315379 1107 5 -0.09423219747758793 1107 6 0.009653788563127819 1107 7 -0.025188272206008833 1107 8 -0.019691683372265345 1107 9 0.1015319115447113 1107 10-11 0.04606432739134192 1107 12-13 -0.05038476634673117 1107 14-15 0.053918053122863796 1107 16-17 0.0522869737767806 1107 18-19 0.08312747500512785 1107 20-21 0.10837187836651196 1107 22-23 0.05608069593893106 1107 24-25 0.09154431036736099 1107 26-27 0.028896346655066907 1107 28-29 -0.043589690673947246 1107 30-31 -0.027414865062930005 1107 32-33 3.6488916585142306E-4 1107 34-35 0.17384803806148597 1107 36-37 0.079738069675507 1107 38-39 -0.020406089469098276 1107 40-41 0.034578349410345766 1107 42-43 0.06822375568152239 1107 44-45 -0.05399277695408955 1107 46-47 -0.02940385184361105 1107 48-49 0.03243157070912872 1107 50-51 0.11776468543421004 1107 52-53 0.1866091616843235 1107 54-55 0.23789498735551007 1107 56-57 0.2330453581867573 1107 58-59 0.08624238815978913 1107 60-61 0.12921363686685794 1107 62-63 0.2093950845528454 1107 64-65 0.24460770547742783 1107 66-67 0.26516035622228884 1107 68-69 0.23444952053202428 1107 70-71 0.3458885834705683 1107 72-73 0.27575781391274035 1107 74-75 0.3741374008498006 1107 76-77 0.30767983654778774 1107 78-79 0.3546066241853971 1107 80-81 0.2276174700841942 1107 82-83 0.21982459245773356 1107 84-85 0.22459832418848436 1107 86-87 0.3792943913373783 1107 88-89 0.5417196817180496 1107 90-91 0.4911398064661867 1107 92-93 0.5880547592309213 1107 94-95 0.7357711289696915 1107 96-97 1.1044659143080402 1107 98-99 1.290700965997459 1107 100-101 1.9808203080940174 1108 1 0.046608205890890986 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-0.7850098248995749 2316 76-77 -0.8603428895745999 2316 78-79 -1.088147292423134 2316 80-81 -0.8362588995459426 2316 82-83 -0.6776872415427633 2316 84-85 -0.8352117926440741 2316 86-87 -0.7333357496583268 2316 88-89 -0.8469304059778899 2316 90-91 -0.6539898228781738 2316 92-93 -1.0369672472786036 2316 94-95 -1.150440087308084 2316 96-97 -1.4087162599791156 2316 98-99 -1.0888887666454572 2316 100-101 -1.5326369475622066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 11.0 5 25.0 6 57.0 7 127.0 8 360.0 9 673.0 10 1251.0 11 2039.0 12 3350.0 13 4897.0 14 6555.0 15 9291.0 16 13291.0 17 19817.0 18 18014.0 19 9768.0 20 7965.0 21 7957.0 22 7945.0 23 8859.0 24 10057.0 25 11945.0 26 14478.0 27 17980.0 28 22520.0 29 28239.0 30 35986.0 31 46057.0 32 59915.0 33 79735.0 34 112242.0 35 165565.0 36 261903.0 37 408849.0 38 325303.0 39 26289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.845631258410556 20.1319724319678 15.341251908012769 21.68114440160888 2 15.349801837172064 21.035385770119685 40.69583649408713 22.91897589862112 3 19.310142975637948 25.454476856422414 30.563607895415302 24.671772272524336 4 12.757771885957842 17.481041182906708 37.531540863353605 32.22964606778184 5 13.2544407116116 38.43493931542031 34.06883989196384 14.24178008100425 6 31.423028046914258 37.121131137316866 17.221730284756525 14.234110531012353 7 28.034738089507975 33.94743897482392 20.484085521279997 17.533737414388114 8 26.55376177815481 35.8658998158712 19.324548581476563 18.255789824497416 9 26.671693384682836 17.066870021991416 20.71943424841324 35.542002344912504 10-11 24.734711050414475 27.655276138886048 27.060187421505166 20.54982538919431 12-13 25.720539020812396 25.0503931408161 28.135693893254583 21.093373945116927 14-15 22.543458340074054 26.09989944658464 26.927421470869522 24.429220742471784 16-17 21.18853107980877 29.811915379642024 27.43324688064327 21.56630665990594 18-19 21.16900919729335 27.643214302251334 30.622973854397056 20.56480264605827 20-21 21.949646076255455 26.75194754525694 30.902451956262944 20.39595442222467 22-23 21.772328532119296 26.816532990494274 30.7983498679054 20.61278860948103 24-25 21.61369339911165 26.87030886463806 31.02731934305526 20.48867839319503 26-27 21.485714939039642 27.24338554015409 30.71541780462673 20.555481716179543 28-29 21.04487500864641 27.175536691932283 31.04266838775474 20.736919911666565 30-31 21.149827546733306 26.96904987639444 31.131823351375637 20.749299225496614 32-33 21.053449942520487 27.141676275167654 30.897309181458617 20.907564600853245 34-35 21.615725891046743 26.979927617547173 30.78017788636607 20.62416860504002 36-37 21.649224641131777 26.73371752093243 30.620029851616543 20.997027986319246 38-39 21.399527473262452 27.107320048544604 30.737789962836963 20.755362515355976 40-41 21.45066165747928 27.16245578993883 30.66953483041115 20.717347722170743 42-43 21.546245583543914 27.088140457596303 30.38636129755194 20.979252661307843 44-45 21.4384854906395 27.47754983510726 30.133783489460757 20.950181184792484 46-47 21.58222714096171 27.6409848632525 29.661028091503038 21.11575990428275 48-49 21.925817989743436 27.737593886535272 29.263616298685374 21.072971825035914 50-51 21.88694572002019 27.97094183507982 28.950037128734095 21.19207531616589 52-53 22.08219943938168 28.10354432311783 28.755598251547674 21.058657985952816 54-55 21.798254063438403 27.97297119621979 28.859516188871215 21.3692585514706 56-57 21.84321441658154 28.40250977666166 28.771567678622368 20.982708128134433 58-59 22.030201466970862 27.95296341033282 28.762478427319433 21.254356695376885 60-61 21.969406380425756 27.835146134009864 29.1246193518735 21.070828133690885 62-63 21.85579073913906 27.89062486601929 29.315722289645286 20.937862105196363 64-65 21.841871036671982 27.729162033911482 29.082174263241868 21.346792666174665 66-67 21.704674790589937 28.11059618057084 28.856257778026762 21.32847125081246 68-69 21.67023281631309 28.14718184619272 28.90559126151377 21.276994075980422 70-71 21.725139897297176 28.16113013121106 28.908135108576538 21.205594862915227 72-73 21.8914966028199 28.0517527254185 28.58175912505356 21.474991546708047 74-75 21.765047359871858 28.4143510744327 28.40803432879719 21.412567236898255 76-77 21.831724230972167 28.233615481224405 28.595727823227215 21.338932464576217 78-79 21.877427730448247 28.200716964715983 28.362465622336465 21.559389682499305 80-81 21.74131762131435 28.572861782213536 28.477310313327642 21.20851028314447 82-83 21.882648518636554 28.512891456239366 28.412652362992997 21.191807662131087 84-85 21.808762560255644 28.34705535158712 28.27602765078381 21.568154437373433 86-87 21.912841511468176 28.085071962289327 28.4922875701916 21.509798956050897 88-89 21.930848518766446 28.080098598368853 28.46064668593893 21.528406196925776 90-91 21.867216939940537 28.09229292504566 28.506141265836245 21.53434886917756 92-93 21.88006371798987 28.081820393129593 28.4144447642603 21.623671124620234 94-95 21.683523702652288 28.085386370353266 28.360636339055368 21.87045358793908 96-97 21.860221034585457 28.00166807769195 28.344201372076792 21.793909515645804 98-99 21.789706767993977 28.208735478954754 28.05030198972226 21.951255763329005 100-101 21.57817795544253 28.257195651279048 28.412245756703975 21.752380636574443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 431.0 1 324.5 2 253.5 3 348.5 4 341.5 5 318.0 6 611.0 7 1458.0 8 2479.5 9 3051.0 10 3028.0 11 2807.0 12 2891.0 13 3138.5 14 3329.0 15 3587.0 16 3776.5 17 3887.0 18 4010.5 19 4115.0 20 4361.0 21 4870.5 22 5727.5 23 6749.5 24 8208.0 25 10212.5 26 12557.5 27 14981.5 28 16959.5 29 19010.0 30 22440.5 31 25922.0 32 29239.0 33 33810.5 34 39203.0 35 43391.0 36 47882.0 37 51791.0 38 54010.0 39 55288.5 40 55666.5 41 58793.5 42 62504.0 43 65855.5 44 69786.0 45 72582.5 46 70893.0 47 67903.0 48 68763.5 49 67165.5 50 63124.0 51 58276.5 52 53071.0 53 50422.0 54 48465.0 55 44387.5 56 38654.0 57 33641.5 58 30109.5 59 26791.5 60 22218.0 61 17229.0 62 13231.0 63 10333.5 64 7767.0 65 5830.0 66 4275.0 67 3372.0 68 2453.0 69 1442.0 70 1030.5 71 789.5 72 588.5 73 404.5 74 284.5 75 225.0 76 154.5 77 91.0 78 60.0 79 39.0 80 24.0 81 16.5 82 8.0 83 5.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04413145915639172 2 0.0 3 0.0 4 5.7165102534186164E-5 5 0.015720403196901194 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00417305248499559 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.574765380127923E-5 22-23 2.572429614038377E-4 24-25 5.144859228076754E-4 26-27 0.0015148752171559332 28-29 0.0021722738962990738 30-31 2.572429614038377E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010232553353619323 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.8582551267093082E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.8582551267093082E-5 74-75 2.8582551267093082E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.574765380127923E-5 84-85 8.574765380127923E-5 86-87 0.0 88-89 0.0 90-91 0.007288550573108735 92-93 0.0027725074729080287 94-95 0.0 96-97 0.0 98-99 2.572429614038377E-4 100-101 0.011833176224576535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.07857103332076 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0910614118672 38.3554411429351 2 13.71565653132602 14.011522728079317 3 4.572722340023447 7.007043685816211 4 2.1117467870853535 4.314600330741044 5 1.1542608613504404 2.947899769873524 6 0.720856210234506 2.209218308356417 7 0.4627840012153851 1.6546841835415116 8 0.310190195518107 1.2675257548489025 9 0.23888769399755724 1.098183784215638 >10 1.4223214254994612 13.605124365476048 >50 0.12731694943379718 4.472798357500402 >100 0.06861963872588407 6.1981550352372325 >500 0.0027937138459674985 0.9559114658643978 >1k 4.4699421535479973E-4 0.4158817065915091 >5k 2.2349710767739987E-4 0.8724040630722789 >10k+ 1.1174855383869993E-4 0.6136053178504972 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10750 0.6145248522425012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9036 0.5165438664989062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6248 0.3571675606335951 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 2554 0.14599967187231144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2066 0.11810310183562861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.858255126709308E-5 0.0 20-21 0.0 0.0 0.0 5.716510253418616E-5 0.0 22-23 0.0 0.0 0.0 8.574765380127923E-5 0.0 24-25 0.0 0.0 0.0 1.714953076025585E-4 0.0 26-27 0.0 0.0 0.0 2.858255126709308E-4 0.0 28-29 0.0 0.0 0.0 8.574765380127923E-4 0.0 30-31 0.0 0.0 0.0 0.0016577879734913986 2.858255126709308E-5 32-33 0.0 0.0 0.0 0.0035156538058524487 5.716510253418616E-5 34-35 0.0 0.0 0.0 0.005173441779343847 5.716510253418616E-5 36-37 0.0 0.0 0.0 0.007031307611704897 5.716510253418616E-5 38-39 0.0 0.0 0.0 0.009289329161805252 5.716510253418616E-5 40-41 0.0 0.0 0.0 0.012261914493582932 5.716510253418616E-5 42-43 0.0 0.0 0.0 0.01689228779885201 5.716510253418616E-5 44-45 0.0 0.0 0.0 0.02409509071815947 5.716510253418616E-5 46-47 0.0 0.0 0.0 0.040644387901806356 5.716510253418616E-5 48-49 0.0 0.0 0.0 0.0722566896032113 5.716510253418616E-5 50-51 0.0 0.0 0.0 0.09895279248667624 5.716510253418616E-5 52-53 0.0 0.0 0.0 0.12630629404928434 5.716510253418616E-5 54-55 0.0 0.0 0.0 0.16066252067233022 5.716510253418616E-5 56-57 0.0 0.0 0.0 0.18901641152928655 5.716510253418616E-5 58-59 0.0 0.0 0.0 0.22145760721743718 5.716510253418616E-5 60-61 0.0 0.0 0.0 0.27101975111457655 5.716510253418616E-5 62-63 0.0 0.0 0.0 0.30900596174854333 5.716510253418616E-5 64-65 0.0 0.0 0.0 0.35373765448154393 5.716510253418616E-5 66-67 0.0 0.0 0.0 0.4345976920161503 5.716510253418616E-5 68-69 0.0 0.0 0.0 0.5220031337909209 5.716510253418616E-5 70-71 0.0 0.0 0.0 0.5985186235329292 5.716510253418616E-5 72-73 0.0 0.0 0.0 0.6971284254044002 1.1433020506837232E-4 74-75 0.0 0.0 0.0 0.7945663426739206 1.1433020506837232E-4 76-77 0.0 0.0 0.0 0.8763124392978068 1.1433020506837232E-4 78-79 0.0 0.0 0.0 0.9824680347037904 1.1433020506837232E-4 80-81 0.0 0.0 0.0 1.1328408369199672 1.1433020506837232E-4 82-83 0.0 0.0 0.0 1.2785261007283406 1.1433020506837232E-4 84-85 0.0 0.0 0.0 1.4357015501460855 1.1433020506837232E-4 86-87 0.0 0.0 0.0 1.7071214569784012 1.1433020506837232E-4 88-89 0.0 0.0 0.0 2.0700626929679493 1.1433020506837232E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5295 0.0 49.549465 1 GTATCAA 9090 0.0 38.43168 1 ATCAACG 11930 0.0 29.306725 3 TATCAAC 12025 0.0 29.193708 2 TCAACGC 12110 0.0 28.87112 4 CAACGCA 12315 0.0 28.390518 5 AACGCAG 12670 0.0 27.629381 6 CGCAGAG 14460 0.0 23.94773 8 ACGCAGA 14470 0.0 23.93118 7 CGTAATA 100 0.0016531848 23.751986 2 GTACATG 8605 0.0 23.530146 1 TACATGG 8825 0.0 22.661951 2 CCTATAC 385 0.0 22.20965 3 GCAGAGT 15360 0.0 21.987886 9 GAGTACT 9190 0.0 21.889862 12-13 ACATGGG 8600 0.0 21.763449 3 AGTACTT 9490 0.0 21.348036 12-13 CGACGTT 90 5.1731913E-6 21.111368 42-43 GTACTTT 9735 0.0 20.565624 14-15 AGAGTAC 14260 0.0 20.452856 10-11 >>END_MODULE