##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937731_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149501 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70763406264841 33.0 31.0 34.0 30.0 34.0 2 31.772677105838756 33.0 31.0 34.0 30.0 34.0 3 31.735493408070848 33.0 31.0 34.0 30.0 34.0 4 35.356271864402245 37.0 35.0 37.0 33.0 37.0 5 35.39271309222012 37.0 35.0 37.0 33.0 37.0 6 35.57340753573555 37.0 35.0 37.0 33.0 37.0 7 35.53653821713567 37.0 35.0 37.0 33.0 37.0 8 35.56819686824837 37.0 35.0 37.0 33.0 37.0 9 37.11609955786249 39.0 37.0 39.0 34.0 39.0 10-11 37.02191958582217 39.0 37.0 39.0 33.5 39.0 12-13 36.947538812449416 39.0 37.0 39.0 33.0 39.0 14-15 37.869428967030316 40.0 38.0 41.0 33.0 41.0 16-17 37.85483709139069 40.0 37.5 41.0 33.0 41.0 18-19 37.92041190359931 40.0 37.0 41.0 33.5 41.0 20-21 37.905127724898165 40.0 37.5 41.0 33.5 41.0 22-23 37.809897592658245 40.0 37.0 41.0 33.0 41.0 24-25 37.71887479013519 40.0 37.0 41.0 33.0 41.0 26-27 37.52089618129645 39.5 36.5 41.0 33.0 41.0 28-29 37.40349228433254 39.0 36.0 41.0 33.0 41.0 30-31 37.139065959425025 39.0 35.5 41.0 33.0 41.0 32-33 36.71148353522719 39.0 35.0 41.0 32.0 41.0 34-35 36.472806201965206 38.5 35.0 41.0 31.5 41.0 36-37 36.15620965746049 38.5 35.0 40.5 30.5 41.0 38-39 35.8670443675962 38.0 35.0 40.0 30.5 41.0 40-41 35.67099885619494 38.0 35.0 40.0 30.0 41.0 42-43 35.23872081123203 38.0 35.0 40.0 28.0 41.0 44-45 34.75319897525769 38.0 35.0 40.0 25.0 41.0 46-47 34.04848796998013 38.0 34.5 40.0 21.0 41.0 48-49 33.373669741339526 38.0 33.0 40.0 9.0 41.0 50-51 32.32148279944616 37.0 32.0 39.5 2.0 40.5 52-53 32.51094975953338 37.5 33.0 39.5 2.0 40.5 54-55 32.82982722523595 38.0 33.0 40.0 2.0 41.0 56-57 32.48620745011739 38.0 33.0 40.0 2.0 41.0 58-59 32.24074755352807 37.0 33.0 40.0 2.0 41.0 60-61 31.761573501180592 36.5 32.0 40.0 2.0 41.0 62-63 31.451221062066473 36.0 31.0 39.5 2.0 41.0 64-65 31.113219978461682 36.0 31.0 39.0 2.0 40.5 66-67 30.506147116072803 35.0 30.5 39.0 2.0 40.0 68-69 29.94414753078575 35.0 29.5 38.0 2.0 40.0 70-71 29.270740663942046 35.0 28.5 37.0 2.0 39.0 72-73 28.76734938227838 34.5 28.0 37.0 2.0 39.0 74-75 28.294870937318144 34.0 26.5 36.0 2.0 38.5 76-77 27.862927338278674 34.0 26.0 35.5 2.0 37.5 78-79 27.265807586571327 34.0 26.0 35.0 2.0 37.0 80-81 26.588838870643006 33.0 24.5 35.0 2.0 36.0 82-83 26.027421221262735 33.0 22.0 35.0 2.0 36.0 84-85 25.48493321114909 32.5 20.0 35.0 2.0 35.5 86-87 25.170637654597627 32.0 18.5 35.0 2.0 35.0 88-89 24.653333422518912 32.0 10.5 35.0 2.0 35.0 90-91 24.04878228239276 31.5 3.5 35.0 2.0 35.0 92-93 23.36010127022562 31.0 2.0 34.0 2.0 35.0 94-95 22.569548029779064 31.0 2.0 34.0 2.0 35.0 96-97 21.7621186480358 30.0 2.0 34.0 2.0 35.0 98-99 20.85322506203972 29.0 2.0 34.0 2.0 35.0 100-101 18.476879753312687 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.319545456936563 1101 2 -0.01001900005709544 1101 3 0.4681895933680309 1101 4 0.23958310974775543 1101 5 0.0108789687146853 1101 6 0.15241346190646965 1101 7 0.317437239735483 1101 8 0.24293717453311103 1101 9 0.39986562238708245 1101 10-11 0.3006100680179813 1101 12-13 0.45455434016975005 1101 14-15 0.289058737630846 1101 16-17 0.6622042919052546 1101 18-19 0.4523162606717648 1101 20-21 0.49243426180973415 1101 22-23 0.5227479244254951 1101 24-25 0.14924062126281257 1101 26-27 0.23189404191755614 1101 28-29 0.05710249653421329 1101 30-31 -0.002093663329468143 1101 32-33 0.44291879835632386 1101 34-35 -0.4786968011082209 1101 36-37 0.0033507184671819346 1101 38-39 -0.30961725848518284 1101 40-41 -0.09189930113252132 1101 42-43 0.0448998912553904 1101 44-45 -0.47190689633549 1101 46-47 -0.460158088430795 1101 48-49 -0.32678501658183734 1101 50-51 -1.0806305247708394 1101 52-53 -0.5774303805350556 1101 54-55 -0.1859295094328104 1101 56-57 -0.37131765774112324 1101 58-59 -0.12480218214373195 1101 60-61 -0.009370616115628394 1101 62-63 0.17510795527729783 1101 64-65 0.07323449469566157 1101 66-67 0.5424436260868255 1101 68-69 0.1342272431046574 1101 70-71 0.28984989368968783 1101 72-73 0.2540165691317604 1101 74-75 0.5631044612279545 1101 76-77 0.8114829117383557 1101 78-79 0.7817811259803555 1101 80-81 1.1746857240309652 1101 82-83 1.1416845108910785 1101 84-85 1.1722336695160251 1101 86-87 1.6113299060416182 1101 88-89 1.3086172158253255 1101 90-91 1.0066131367344298 1101 92-93 0.6431887842090944 1101 94-95 0.8628605095561426 1101 96-97 1.1398651573021432 1101 98-99 0.7302412098922417 1101 100-101 1.0281160055385463 1102 1 -0.09559753415579308 1102 2 0.3161257100538606 1102 3 0.5553125813348956 1102 4 0.6247214126075775 1102 5 0.5372893453360348 1102 6 0.21976773601179644 1102 7 0.3634600558004948 1102 8 0.3269346741424286 1102 9 0.4855040033841149 1102 10-11 -0.001663724762146046 1102 12-13 0.338520584895484 1102 14-15 0.11801638726359442 1102 16-17 0.6273160281140377 1102 18-19 0.6195689564054732 1102 20-21 0.6221029600438328 1102 22-23 0.6508148098763442 1102 24-25 0.730229838327979 1102 26-27 0.4670089036146976 1102 28-29 0.5762461127242418 1102 30-31 0.7746292621276325 1102 32-33 0.6463490218287475 1102 34-35 0.6271009797950455 1102 36-37 1.3156651425959538 1102 38-39 1.4340536275907638 1102 40-41 1.1173521444058494 1102 42-43 0.7545420228384572 1102 44-45 0.5628250930378513 1102 46-47 0.9556490689375465 1102 48-49 0.8434368930915497 1102 50-51 0.6777301565856249 1102 52-53 0.4788674785054212 1102 54-55 0.5723139661102437 1102 56-57 0.6610702153640489 1102 58-59 0.43520719432134314 1102 60-61 0.8368553566926238 1102 62-63 1.0514948907359667 1102 64-65 0.5282274623468588 1102 66-67 0.8700729431676208 1102 68-69 0.7266635612793735 1102 70-71 0.9469447522613414 1102 72-73 0.3382094117634189 1102 74-75 0.5336076648535197 1102 76-77 0.8180073686847535 1102 78-79 0.5622546374665269 1102 80-81 0.3629573289692374 1102 82-83 0.508694981277074 1102 84-85 0.8378845524664875 1102 86-87 0.7461165914612131 1102 88-89 0.4289875861956993 1102 90-91 0.0793588782565422 1102 92-93 0.4484317909288933 1102 94-95 0.6120400688622816 1102 96-97 0.5862630319700592 1102 98-99 0.4503537274731144 1102 100-101 0.6998225221817727 1103 1 0.1525957811106906 1103 2 0.3695734667536499 1103 3 0.1491096304165005 1103 4 0.26451364386322496 1103 5 0.3731821487085085 1103 6 0.22172497780643852 1103 7 0.3947759245147324 1103 8 0.3338602989542281 1103 9 0.12431761374244132 1103 10-11 0.08291170432548256 1103 12-13 0.36189456863532854 1103 14-15 0.46550208434279483 1103 16-17 0.08459546201947887 1103 18-19 -0.0757788304057243 1103 20-21 0.11596775980294183 1103 22-23 0.42896898030694075 1103 24-25 0.7951575709386418 1103 26-27 0.613802038212711 1103 28-29 0.7683945619865113 1103 30-31 1.0314043298783133 1103 32-33 1.096476995330704 1103 34-35 1.260381618156984 1103 36-37 1.3258653834872476 1103 38-39 1.1139316763712515 1103 40-41 0.7151013163420075 1103 42-43 1.004918415491268 1103 44-45 1.5536411123091511 1103 46-47 1.3952756874259507 1103 48-49 1.4635437886049445 1103 50-51 0.9713917043122109 1103 52-53 1.3733646179212613 1103 54-55 1.538175152499889 1103 56-57 1.1781960810671492 1103 58-59 1.1256995159112222 1103 60-61 1.1316837216429434 1103 62-63 1.5590227437922692 1103 64-65 1.268742367495907 1103 66-67 1.302284626395565 1103 68-69 1.6431420316196323 1103 70-71 1.6735331292562243 1103 72-73 1.422973037177453 1103 74-75 1.1184842080633963 1103 76-77 1.3531624424577124 1103 78-79 1.470226633853155 1103 80-81 1.327124748421209 1103 82-83 0.8287792932313067 1103 84-85 1.3810944290096963 1103 86-87 1.689093104559678 1103 88-89 2.1470318499716647 1103 90-91 2.160132741550729 1103 92-93 1.3317500685561185 1103 94-95 0.9180472956012196 1103 96-97 0.6641010325722867 1103 98-99 0.5899201231370697 1103 100-101 0.6864982222721032 1104 1 0.09674511524206864 1104 2 0.2724613913384175 1104 3 0.19759085142502997 1104 4 0.2563960320122547 1104 5 0.5191495286277075 1104 6 0.15326769814161878 1104 7 0.3086619038653282 1104 8 0.4377806939863973 1104 9 0.6903059423373819 1104 10-11 -0.10328835552787297 1104 12-13 0.14322406137810617 1104 14-15 0.06583127781877351 1104 16-17 0.3726958660296589 1104 18-19 0.28509951764155517 1104 20-21 0.1650288102293942 1104 22-23 0.6051623066573839 1104 24-25 0.5006229308069621 1104 26-27 0.17889256641861095 1104 28-29 0.6775109767702929 1104 30-31 0.6031909526525112 1104 32-33 0.9418121741426191 1104 34-35 0.9763587242470635 1104 36-37 1.110673852737584 1104 38-39 1.2699431981429115 1104 40-41 1.2079376174044043 1104 42-43 1.4663878154613386 1104 44-45 1.6993092254320032 1104 46-47 1.2197557113672985 1104 48-49 1.1216501779510466 1104 50-51 1.619940260349921 1104 52-53 1.525485671340384 1104 54-55 1.5490048696927659 1104 56-57 1.4426918165152998 1104 58-59 1.4896266444463109 1104 60-61 1.3916156384467655 1104 62-63 1.2884107431937366 1104 64-65 1.767320850213256 1104 66-67 1.6319831150982473 1104 68-69 1.416241687462815 1104 70-71 1.363081236393736 1104 72-73 1.2060046098244719 1104 74-75 1.3968584411922293 1104 76-77 1.234465749355813 1104 78-79 0.9898847215383277 1104 80-81 1.2498390982640935 1104 82-83 0.5577558007877919 1104 84-85 0.6863091529360759 1104 86-87 1.2220311563328359 1104 88-89 1.2319300992609108 1104 90-91 1.462018122367727 1104 92-93 1.618260617710689 1104 94-95 1.6754915571603952 1104 96-97 1.1300220261380538 1104 98-99 1.2920374357212374 1104 100-101 2.1072299295891774 1105 1 0.030820791684497806 1105 2 0.20462210625738209 1105 3 0.2813526869511094 1105 4 0.15415836400984517 1105 5 0.14697321031854926 1105 6 0.2068511585480124 1105 7 0.26951157938573544 1105 8 -0.07915253059603344 1105 9 0.209260515194579 1105 10-11 -0.014455418189669444 1105 12-13 0.12079418797834052 1105 14-15 -0.055786510046040405 1105 16-17 -0.1069842644714214 1105 18-19 0.3669463916441771 1105 20-21 0.038091684799411496 1105 22-23 -0.013086567416166872 1105 24-25 0.41413050199743395 1105 26-27 0.2612713741777455 1105 28-29 0.12577155338648538 1105 30-31 0.18567614716296532 1105 32-33 0.5785191495672777 1105 34-35 0.7035121694046893 1105 36-37 0.3847438552199449 1105 38-39 0.3719148992670611 1105 40-41 -0.13166662388074002 1105 42-43 -0.4718192131566923 1105 44-45 0.133438095250078 1105 46-47 0.007921958397332673 1105 48-49 -0.365601143017777 1105 50-51 -0.23913275642372156 1105 52-53 -0.3269913668524609 1105 54-55 0.05616824428051359 1105 56-57 0.004697561399872541 1105 58-59 0.08014403868015307 1105 60-61 0.18474660053762193 1105 62-63 0.6456574540700082 1105 64-65 0.6016057539332422 1105 66-67 0.5527604476277652 1105 68-69 0.5556437939204919 1105 70-71 0.8841280820265602 1105 72-73 0.655878718835428 1105 74-75 0.7232529941905632 1105 76-77 0.749856366044348 1105 78-79 0.9624901330777469 1105 80-81 0.112546995707266 1105 82-83 0.19346983320028954 1105 84-85 0.6484354694721226 1105 86-87 0.7826184112000298 1105 88-89 0.6727790655363073 1105 90-91 0.6233358995938687 1105 92-93 0.5235941401760229 1105 94-95 1.47486387532771 1105 96-97 1.221869254763181 1105 98-99 1.7026016401256499 1105 100-101 1.994513166583399 1106 1 0.2683755298172734 1106 2 0.6776296382244524 1106 3 0.7921251514808034 1106 4 0.3755631634493284 1106 5 0.5138325552125735 1106 6 0.4711706540813836 1106 7 0.4859067449138479 1106 8 0.509987423861844 1106 9 0.6257958103425878 1106 10-11 0.5577638846654693 1106 12-13 0.17791452428942733 1106 14-15 0.9027526386665201 1106 16-17 0.6848356689670112 1106 18-19 0.18892922576574023 1106 20-21 0.3008347221089238 1106 22-23 0.3397598154880228 1106 24-25 0.41642512452326486 1106 26-27 0.2585913951971861 1106 28-29 0.8006007704122311 1106 30-31 0.9029120904104673 1106 32-33 0.90987315201955 1106 34-35 1.1773254466861829 1106 36-37 1.0293576229550965 1106 38-39 0.9416293851665216 1106 40-41 1.3827842431712796 1106 42-43 0.9399516749147736 1106 44-45 1.227920491466584 1106 46-47 0.9323045122596483 1106 48-49 0.8376568706448566 1106 50-51 0.7913104034992031 1106 52-53 1.250360183331459 1106 54-55 1.0062645833941986 1106 56-57 1.0108457484729172 1106 58-59 1.05709271620686 1106 60-61 1.2112661311034003 1106 62-63 1.5445364171108196 1106 64-65 1.3701466542660548 1106 66-67 1.4887036951317079 1106 68-69 0.949110250392728 1106 70-71 1.1741613628112866 1106 72-73 1.5130691199564623 1106 74-75 1.5313629959421853 1106 76-77 1.3552688726129247 1106 78-79 1.523534098745987 1106 80-81 0.6368069361521762 1106 82-83 1.5298509857664122 1106 84-85 1.5765489744642416 1106 86-87 1.2323118776565032 1106 88-89 0.914677821885931 1106 90-91 0.8982702138345431 1106 92-93 1.2487123745428121 1106 94-95 1.4243296311518456 1106 96-97 1.109888172261865 1106 98-99 1.8296466567660445 1106 100-101 1.5041996265588757 1107 1 -0.08749568230394189 1107 2 0.011341759436575671 1107 3 0.11542060602625526 1107 4 -0.09981562442946057 1107 5 -0.3695007781207593 1107 6 -0.17598843682770848 1107 7 -0.2311387096316082 1107 8 -0.09891409128967155 1107 9 0.26197005276682717 1107 10-11 0.08352146042304298 1107 12-13 0.11768725156215254 1107 14-15 0.3055935477574181 1107 16-17 0.33672960836094745 1107 18-19 0.08230043788695696 1107 20-21 0.12214154029074109 1107 22-23 0.13133178518499022 1107 24-25 0.12930391240205807 1107 26-27 -0.17720405934827 1107 28-29 -0.28008104776958476 1107 30-31 0.4645598176831882 1107 32-33 0.18496784898923835 1107 34-35 0.5498633254740568 1107 36-37 0.36458489568236985 1107 38-39 0.03405362759075814 1107 40-41 -0.11721575682872043 1107 42-43 -0.14584377963068818 1107 44-45 -0.36213632671523754 1107 46-47 -0.09496821501307551 1107 48-49 -0.22342267480968303 1107 50-51 -0.4232729298341269 1107 52-53 -0.8754920893958129 1107 54-55 -0.6333187499391357 1107 56-57 -0.5064837969816267 1107 58-59 -0.8475474353082895 1107 60-61 -0.5997187173814496 1107 62-63 -0.1937211586467562 1107 64-65 -0.5245503154309183 1107 66-67 -0.538569032141023 1107 68-69 -0.4689768708193931 1107 70-71 -0.8321833341584117 1107 72-73 -0.7861733042859633 1107 74-75 -0.9940157919366044 1107 76-77 -0.25240158193253137 1107 78-79 -0.4121666588297721 1107 80-81 -0.5440642759690348 1107 82-83 -0.700641438476012 1107 84-85 -1.3439055709903052 1107 86-87 -1.3766843344647093 1107 88-89 -1.1757577841746745 1107 90-91 -1.394059331620003 1107 92-93 -1.4725944436390037 1107 94-95 -1.7109923385451218 1107 96-97 -1.6528959186472285 1107 98-99 -0.7520768280824441 1107 100-101 -0.3646450704108197 1108 1 -0.5982911368493973 1108 2 -0.2557036951088776 1108 3 0.02848878784443798 1108 4 0.005866193752360971 1108 5 0.010802252182273264 1108 6 0.14389792680865554 1108 7 -0.1807599217528164 1108 8 -0.2172853034108826 1108 9 -0.08329509874832297 1108 10-11 -0.07556944866786353 1108 12-13 0.07185391822881826 1108 14-15 0.09972233563620847 1108 16-17 -0.08183099769965452 1108 18-19 -0.08076774393122577 1108 20-21 0.14022866150286006 1108 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4 104.0 5 118.0 6 122.0 7 138.0 8 194.0 9 242.0 10 333.0 11 539.0 12 787.0 13 1081.0 14 1408.0 15 1970.0 16 2553.0 17 3560.0 18 2739.0 19 1534.0 20 1494.0 21 1473.0 22 1504.0 23 1621.0 24 1841.0 25 2025.0 26 2282.0 27 2459.0 28 2994.0 29 3430.0 30 4244.0 31 5415.0 32 6615.0 33 8262.0 34 11797.0 35 17294.0 36 18094.0 37 22700.0 38 14272.0 39 1277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.945514584479916 21.595500489952617 16.436903499469775 15.022081426097694 2 14.722930363611969 26.035153629410974 36.09888635448812 23.14302965248893 3 23.79482913608737 27.218144982759313 34.33110165966753 14.655924221485787 4 10.561447997799558 19.83416185320104 41.572242236399866 28.03214791259954 5 8.106947566171291 42.86691938676238 35.495320205307124 13.530812841759202 6 22.778786802404678 40.01702310182362 17.146418781708878 20.057771314062826 7 19.831104855735397 40.24865118461178 22.6889179317047 17.231326027948125 8 23.284274301646025 28.735054420674494 21.014958983432525 26.965712294246956 9 21.88369178389754 21.374332303444206 27.593879643716445 29.148096268941803 10-11 23.73134128276796 27.146045726005912 26.366183400025474 22.75642959120066 12-13 28.353457260531968 28.466392538899 26.787778861330892 16.39237133923814 14-15 22.30923896652257 29.854562514661037 32.785094333299824 15.05110418551657 16-17 15.230563002680967 32.87969168900805 32.56233243967828 19.327412868632706 18-19 14.297825853841617 33.64430861615999 35.54970242882419 16.5081631011742 20-21 17.926981276219326 28.104099931645464 34.03988687995068 19.92903191218453 22-23 16.132177572567326 28.238514711154743 36.94659353252001 18.68271418375792 24-25 20.156350525412826 29.18956412180999 34.282851704910996 16.37123364786618 26-27 17.27827106718043 26.691238063327187 40.845702797788576 15.184788071703803 28-29 14.398790913081797 33.87554581494402 36.17676529035847 15.548897981615713 30-31 15.812209278944758 29.034236383865736 35.85336264561187 19.300191691577634 32-33 16.19491269814672 30.02077817621234 37.64167699989946 16.14263212574148 34-35 18.8687591739891 29.001869985723765 35.26531679166751 16.86405404861963 36-37 16.687105875099682 28.029191210470234 39.18698860095294 16.096714313477143 38-39 16.208800412749678 31.052712690042416 36.89151249974873 15.846974397459176 40-41 15.595829228903229 32.03599836492907 36.339451447104786 16.028720959062916 42-43 14.962437255808656 31.64351246841848 32.941286850693956 20.452763425078913 44-45 16.963067962856844 28.990358999105265 33.79846052283245 20.248112515205438 46-47 20.193615837762124 31.338087163987048 34.189380281878925 14.278916716371898 48-49 18.02334932847224 29.996582044339597 34.52537329437311 17.454695332815053 50-51 13.30871486926188 31.99808324481186 37.47725528699387 17.21594659893238 52-53 16.82940318769169 34.02182110714465 30.87703420651281 18.271741498650844 54-55 15.325874622624754 29.918275851670167 33.529685735634615 21.226163790070466 56-57 19.0637793218045 29.732862531742256 31.3677143565451 19.83564378990814 58-59 22.01104890934735 29.272909400953463 31.53461913424525 17.181422555453935 60-61 18.15136409926569 30.677828697003807 35.831390362866486 15.33941684086402 62-63 15.765898568107048 31.19342280709983 35.11487975985473 17.92579886493839 64-65 15.30529517785714 34.285656855322316 32.938914163578985 17.470133803241563 66-67 17.63348140405888 30.74913067074029 29.92033660964939 21.697051315551448 68-69 15.706541404773711 30.28632885267432 33.27592907782409 20.73120066472788 70-71 20.295958296480933 30.412277880517806 30.841775883787626 18.449987939213635 72-73 18.066392182218365 27.498919594370502 36.82725905279415 17.607429170616985 74-75 15.293155953405943 34.528447903300915 32.215495840773485 17.962900302519657 76-77 16.177332837466118 30.205684380517777 31.992146535462883 21.62483624655322 78-79 16.869641546919866 30.379560234121893 33.48365200668728 19.267146212270962 80-81 19.181858991109056 29.01114215459656 31.825825946547138 19.981172907747247 82-83 16.52836357910747 28.596745717741097 35.75958378420028 19.115306918951152 84-85 16.640481845376677 30.379640826882042 33.32417702055815 19.655700307183128 86-87 15.974555406306212 31.70324019951027 32.654230213947635 19.667974180235888 88-89 15.613694224842556 30.776497387109742 30.352740184912236 23.257068203135468 90-91 16.6873816981859 27.451046081173892 32.42198361781604 23.43958860282417 92-93 19.046231253412657 29.35739864197159 33.26700634126242 18.32936376335333 94-95 17.243377788497938 28.106250188440317 33.74036441844805 20.91000760461369 96-97 13.870361499060325 29.164140204417315 35.83031553698908 21.13518275953328 98-99 16.71893007424084 30.126839269920396 31.049824448554048 22.104406207284715 100-101 15.70399348876779 26.460589293310242 33.892906307253796 23.942510910668172 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2904.0 1 1772.0 2 624.5 3 579.0 4 441.5 5 485.0 6 877.0 7 1542.0 8 2059.0 9 2036.0 10 1478.5 11 949.5 12 863.5 13 819.5 14 734.0 15 684.5 16 683.0 17 697.5 18 719.0 19 762.5 20 808.5 21 881.0 22 931.5 23 969.5 24 1076.5 25 1273.0 26 1714.5 27 2344.5 28 2500.0 29 2600.5 30 2820.0 31 2950.5 32 3280.5 33 3569.0 34 3939.0 35 4334.0 36 4790.5 37 5069.5 38 4503.5 39 3590.0 40 3071.5 41 2747.0 42 2389.0 43 2253.5 44 2317.0 45 2331.0 46 3019.0 47 7699.5 48 16245.0 49 15648.5 50 7127.0 51 2644.5 52 1299.5 53 967.5 54 783.5 55 640.0 56 575.0 57 567.5 58 541.5 59 481.0 60 446.0 61 427.5 62 345.5 63 215.0 64 121.0 65 82.0 66 72.5 67 63.0 68 49.0 69 34.5 70 20.0 71 15.0 72 13.0 73 10.0 74 6.0 75 3.0 76 4.0 77 6.0 78 3.5 79 2.0 80 3.0 81 1.5 82 0.5 83 0.0 84 1.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33912816636678017 2 0.2949813044728798 3 0.2909679533916161 4 0.2943124126260025 5 0.30501468217603894 6 0.1953164192881653 7 0.19799198667567441 8 0.19598531113504258 9 0.19665420298191988 10-11 0.20601868883820176 12-13 0.20100199998662216 14-15 0.19799198667567441 16-17 0.20133644591006078 18-19 0.19598531113504258 20-21 0.1866208252787607 22-23 0.18561748750844476 24-25 0.19063417636002436 26-27 0.18461414973812884 28-29 0.19899532444599033 30-31 0.20267422960381534 32-33 0.2046809051444472 34-35 0.20200533775693807 36-37 0.1859519334318834 38-39 0.17257409649433783 40-41 0.18193858235061972 42-43 0.18996528451314706 44-45 0.1969886489053585 46-47 0.1926408519006562 48-49 0.19330974374753346 50-51 0.1943130815178494 52-53 0.22307543093357235 54-55 0.18695527120219932 56-57 0.16856074541307417 58-59 0.17090186687714462 60-61 0.16387850248493321 62-63 0.17257409649433783 64-65 0.16856074541307417 66-67 0.16588517802556504 68-69 0.17859412311623332 70-71 0.17190520464746056 72-73 0.16822629948963552 74-75 0.17023297503026735 76-77 0.17892856903967197 78-79 0.1755841098052856 80-81 0.16588517802556504 82-83 0.16822629948963552 84-85 0.16153738102086274 86-87 0.15752402993959908 88-89 0.16120293509742412 90-91 0.16956408318339009 92-93 0.16086848917398547 94-95 0.16688851579588096 96-97 0.16555073210212642 98-99 0.17190520464746056 100-101 0.1474906522364399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.0940997050187 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10006044059107 25.38645226453335 2 9.91017277672412 6.361161463802918 3 3.493049332027261 3.3631882060989557 4 1.8111335737062588 2.3250680597454196 5 1.0837623225860236 1.7391188018809238 6 0.7732227339988745 1.4889532511488217 7 0.523123736479023 1.1752429749633782 8 0.42725245409641316 1.0969826288787365 9 0.3313811717138034 0.9571842328813853 >10 2.2050394948000247 13.665460431702797 >50 0.17298513995123072 3.8233857967505234 >100 0.11462870719659865 7.194600705012006 >500 0.014589108188658011 3.614022648677935 >1k 0.03751484962797774 24.15970461736042 >5k 0.00208415831266543 3.6494739165624313 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5456 3.6494739165624313 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4409 2.9491441528819204 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2956 1.9772442993692347 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2884 1.929084086394071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2884 1.929084086394071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2447 1.6367783493087003 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 1.4762443060581534 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1966 1.3150413709607294 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1714 1.1464806255476552 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1686 1.1277516538350913 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1672 1.1183871679788095 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1626 1.0876181430224547 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1574 1.0528357669848363 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1527 1.0213978501816041 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1518 1.0153778235597086 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1452 0.9712309616658082 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1298 0.8682216172467073 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1251 0.8367837004434753 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1048 0.7009986555273877 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 995 0.665547387642892 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 953 0.6374539300740463 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 849 0.5678891779988093 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 743 0.4969866422298179 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 634 0.42407743092019456 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 626 0.41872629614517626 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.40334178366699885 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.3337770315917619 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 481 0.32173697834797094 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 470 0.31437916803232085 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 423 0.28294125122908875 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 403 0.2695634142915432 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 352 0.235449930100802 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.2294299034789065 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 293 0.19598531113504258 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 282 0.1886275008193925 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.17926301496311062 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 266 0.17792523126935605 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 265 0.1772563394224788 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 264 0.17658744757560152 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 263 0.17591855572872422 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 256 0.17123631280058327 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.14782509815987854 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 212 0.14180507153798302 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 205 0.13712282860984207 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 193 0.12909612644731475 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 190 0.1270894509066829 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.11839385689727827 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 171 0.11438050581601461 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 170 0.11371161396913733 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 169 0.11304272212226006 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 167 0.11170493842850548 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 165 0.11036715473475094 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 161 0.10769158734724182 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.344459234386392E-4 0.0 16-17 0.0 0.0 0.0 0.0013377836937545568 0.0 18-19 0.0 0.0 0.0 0.0013377836937545568 0.0 20-21 0.0 0.0 0.0 0.0013377836937545568 0.0 22-23 0.0 0.0 0.0 0.0013377836937545568 0.0 24-25 0.0 0.0 0.0 0.003344459234386392 0.0 26-27 0.0 0.0 0.0 0.005685580698456866 0.0 28-29 0.0 0.0 0.0 0.012374499167229651 0.0 30-31 0.0 0.0 0.0 0.03712349750168895 0.0 32-33 0.0 0.0 0.0 0.06020026621895506 0.0 34-35 0.0 0.0 0.0 0.08762483194092346 0.0 36-37 0.0 0.0 0.0 0.11404605989257596 0.0 38-39 0.0 0.0 0.0 0.15652069216928316 0.0 40-41 0.0 0.0 0.0 0.2508344425789794 0.0 42-43 0.0 0.0 0.0 0.33411147751520054 0.0 44-45 0.0 0.0 0.0 0.42742189015458093 0.0 46-47 0.0 0.0 0.0 0.5708991913097572 0.0 48-49 0.0 0.0 0.0 0.6618684824850669 0.0 50-51 0.0 0.0 0.0 0.8013324325589795 0.0 52-53 0.0 0.0 0.0 0.9324352345469261 0.0 54-55 0.0 0.0 0.0 1.0839392378646298 0.0 56-57 0.0 0.0 0.0 1.4019973110547754 0.0 58-59 0.0 0.0 0.0 1.6227316205242774 0.0 60-61 0.0 0.0 0.0 1.8277469715921633 0.0 62-63 0.0 0.0 0.0 2.044133484056963 0.0 64-65 0.0 0.0 0.0 2.254165523976428 0.0 66-67 0.0 0.0 0.0 2.581922528946295 0.0 68-69 0.0 0.0 0.0 2.9825887452257844 0.0 70-71 0.0 0.0 0.0 3.3197102360519324 0.0 72-73 0.0 0.0 0.0 3.638437201088956 0.0 74-75 0.0 0.0 0.0 4.118032655299965 0.0 76-77 0.0 0.0 0.0 4.535755613674825 0.0 78-79 0.0 0.0 0.0 5.166520625280098 0.0 80-81 0.0 0.0 0.0 5.779560002943124 0.0 82-83 0.0 0.0 0.0 6.295944508732383 0.0 84-85 0.0 0.0 0.0 6.978883084394084 0.0 86-87 0.0 0.0 0.0 7.5440966950053845 0.0 88-89 0.0 0.0 0.0 8.273857699948495 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 25 0.0016160257 37.98981 94-95 TCTAGAT 55 0.0025673865 34.55936 3 CTTTGCG 85 1.6182665E-5 33.54291 2 CGCAGAA 100 1.2884902E-6 33.25223 8 CGGTGTT 60 0.0039258124 31.690042 5 TTCCGGT 60 0.00393227 31.679415 2 TCCGGTG 60 0.00393227 31.679415 3 TGGGGAG 75 3.0645033E-4 31.66879 6 TTGGTCT 30 0.0039450075 31.668789 24-25 AGCCCCC 30 0.0039514895 31.658175 86-87 ATGGGGT 110 2.696892E-6 30.249588 5 GCTTTGC 95 3.453264E-5 30.022148 1 CATGGGT 95 3.4691304E-5 30.002014 6 GGTGTTG 65 0.005826077 29.23273 6 TTTGCGT 100 4.911139E-5 28.511475 3 CTATTCA 70 0.008338489 27.162895 4 ACATATC 35 0.008379041 27.144678 70-71 ACCTTCA 35 0.008379041 27.144678 36-37 CATGGGG 565 0.0 26.922518 4 ATGGGGA 360 0.0 26.40837 5 >>END_MODULE