##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937731_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 149501 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76387448913385 31.0 31.0 34.0 30.0 34.0 2 32.014989866288516 31.0 31.0 34.0 30.0 34.0 3 32.035257289248904 33.0 31.0 34.0 30.0 34.0 4 35.75978087103096 37.0 35.0 37.0 35.0 37.0 5 35.68442351556177 37.0 35.0 37.0 33.0 37.0 6 35.81298452853158 37.0 35.0 37.0 35.0 37.0 7 35.77030253978234 37.0 35.0 37.0 33.0 37.0 8 35.792496371261734 37.0 35.0 37.0 33.0 37.0 9 37.31035243911412 39.0 37.0 39.0 34.0 39.0 10-11 37.242894696356544 39.0 37.0 39.0 33.5 39.0 12-13 37.1661995571936 39.0 37.0 39.0 33.0 39.0 14-15 37.985297757205636 40.0 37.0 41.0 33.0 41.0 16-17 38.00815379161343 39.0 37.0 41.0 33.0 41.0 18-19 38.1145443843185 40.0 37.0 41.0 33.5 41.0 20-21 38.09473180781399 40.0 37.0 41.0 33.5 41.0 22-23 37.998043491347886 40.0 37.0 41.0 33.0 41.0 24-25 37.87916134340239 40.0 36.5 41.0 33.0 41.0 26-27 37.57490250901331 39.0 36.0 41.0 33.0 41.0 28-29 37.42221122266741 39.0 36.0 41.0 33.0 41.0 30-31 37.32087745232474 39.0 35.5 41.0 33.0 41.0 32-33 36.940194379970706 39.0 35.0 41.0 33.0 41.0 34-35 36.56963498571916 38.0 35.0 40.0 31.5 41.0 36-37 36.25150333442586 38.0 35.0 40.0 30.5 41.0 38-39 35.99037464632344 38.0 35.0 40.0 31.0 41.0 40-41 35.86689721138989 38.0 35.0 40.0 30.5 41.0 42-43 35.440689359937394 38.0 35.0 40.0 29.5 41.0 44-45 34.90870629627896 38.0 35.0 40.0 26.0 41.0 46-47 34.12605266854402 38.0 34.0 40.0 21.5 41.0 48-49 33.41052568210246 38.0 33.0 40.0 12.5 41.0 50-51 32.975799492979974 38.0 33.0 40.0 5.0 41.0 52-53 32.87384030876048 38.0 33.0 40.0 2.0 41.0 54-55 32.68586832195102 37.5 33.0 40.0 2.0 41.0 56-57 32.27108514324319 37.0 32.5 40.0 2.0 41.0 58-59 32.022732289416126 37.0 32.0 40.0 2.0 41.0 60-61 31.433853285262305 36.0 31.0 39.0 2.0 41.0 62-63 31.143577634932207 36.0 31.0 39.0 2.0 40.0 64-65 30.737968307904296 35.0 30.5 39.0 2.0 40.0 66-67 30.178677734597095 35.0 29.5 38.0 2.0 40.0 68-69 29.629624551006348 35.0 29.0 37.0 2.0 39.0 70-71 29.044979632243262 34.0 28.5 37.0 2.0 39.0 72-73 28.482257643761578 34.0 27.0 36.0 2.0 38.5 74-75 28.025799158534056 34.0 26.0 35.5 2.0 37.5 76-77 26.89994046862563 32.0 26.0 34.5 2.0 36.0 78-79 27.477174065725315 33.5 26.0 35.0 2.0 36.0 80-81 27.194005391268284 33.0 26.0 35.0 2.0 36.0 82-83 26.765523307536405 33.0 25.5 35.0 2.0 36.0 84-85 26.357024367729984 33.0 25.0 35.0 2.0 35.0 86-87 26.172543996361227 33.0 24.5 35.0 2.0 35.0 88-89 25.617895532471355 32.5 21.5 35.0 2.0 35.0 90-91 25.01679921873432 32.0 18.5 34.0 2.0 35.0 92-93 24.54789934515488 32.0 14.0 34.0 2.0 35.0 94-95 23.920763071818918 31.0 3.5 34.0 2.0 35.0 96-97 23.1390057591588 31.0 2.0 34.0 2.0 35.0 98-99 22.304847459214322 31.0 2.0 34.0 2.0 35.0 100-101 19.847860549427764 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2164272404916332 1101 2 0.2568037891424808 1101 3 0.33954274963627284 1101 4 0.14818690528093725 1101 5 0.30291314315973494 1101 6 0.35673104396329336 1101 7 0.25874545302918506 1101 8 0.13934825123219952 1101 9 0.14128063999137908 1101 10-11 0.3954750555428319 1101 12-13 0.7051453461783268 1101 14-15 0.407746237710235 1101 16-17 0.4390881616935616 1101 18-19 0.2953246938421259 1101 20-21 0.21914182176061558 1101 22-23 0.16246041640420117 1101 24-25 0.08112466949569352 1101 26-27 -0.19045703543874737 1101 28-29 -0.20951225472562385 1101 30-31 0.16652499837729096 1101 32-33 -0.6118040933576552 1101 34-35 -0.41356296620215005 1101 36-37 -0.027507622219552275 1101 38-39 -0.2968645959596259 1101 40-41 -0.3423929502962153 1101 42-43 0.5935251888542368 1101 44-45 0.5861547015819966 1101 46-47 0.43144251594079464 1101 48-49 0.7992857324941909 1101 50-51 1.3480617438410079 1101 52-53 0.8499210611429575 1101 54-55 1.2958455381411085 1101 56-57 0.8561266835360684 1101 58-59 0.761313609631582 1101 60-61 1.0147534898228123 1101 62-63 1.1670589550539567 1101 64-65 1.2223965687778886 1101 66-67 0.7741508524910472 1101 68-69 0.8951572416294731 1101 70-71 0.7767920786689331 1101 72-73 1.0638307023450473 1101 74-75 1.471768341085376 1101 76-77 1.1600839188574632 1101 78-79 1.268977972329715 1101 80-81 0.9657309434004056 1101 82-83 1.2955154304282388 1101 84-85 1.283747486897994 1101 86-87 1.2890633422731277 1101 88-89 1.3239621215834454 1101 90-91 1.1322057772426426 1101 92-93 1.110522508840866 1101 94-95 1.3823996630546418 1101 96-97 1.9013056653008604 1101 98-99 0.7114630776010209 1101 100-101 1.201416430891836 1102 1 0.08124986902734221 1102 2 -0.058245437299149216 1102 3 -0.175225182853179 1102 4 -0.12123019114037703 1102 5 -0.17841206390386333 1102 6 0.012849187423213948 1102 7 0.17170060227125816 1102 8 0.13473815590480598 1102 9 0.06244019617203378 1102 10-11 0.06542348498493311 1102 12-13 -0.0879473245514717 1102 14-15 0.1479009493839314 1102 16-17 -0.2789537198504277 1102 18-19 -0.3224671486332653 1102 20-21 -0.09692318839721992 1102 22-23 -0.31105106976551156 1102 24-25 -0.35566232847273227 1102 26-27 -0.15924122046765632 1102 28-29 -0.5338988776354512 1102 30-31 -0.1323107572095168 1102 32-33 -0.2023260499133599 1102 34-35 -0.361601722257781 1102 36-37 -0.2453150921367282 1102 38-39 -0.44458298945861685 1102 40-41 -0.3530977479208488 1102 42-43 0.2850785565679459 1102 44-45 0.5962344015351064 1102 46-47 0.8255275292241251 1102 48-49 0.14164391345996563 1102 50-51 -0.5557513519552018 1102 52-53 0.09945223788933077 1102 54-55 0.07061503337474306 1102 56-57 0.23407636317350722 1102 58-59 -0.15332148960267133 1102 60-61 -0.11620447235878117 1102 62-63 -0.46440689898573595 1102 64-65 -0.5203757393952628 1102 66-67 -0.6455631653242406 1102 68-69 -0.1673134569171708 1102 70-71 -0.2214107655254729 1102 72-73 -0.1149081631026796 1102 74-75 -0.18466235121029584 1102 76-77 -0.30244928945633376 1102 78-79 -0.030287537906261264 1102 80-81 -0.18229999893433657 1102 82-83 -0.31422827952644283 1102 84-85 0.11751057488049454 1102 86-87 -0.08513575133524753 1102 88-89 -0.19936183528481521 1102 90-91 0.11845362846939267 1102 92-93 0.02863471387290417 1102 94-95 -0.8747108229588001 1102 96-97 -0.6190490776277215 1102 98-99 0.17930961612266927 1102 100-101 -0.41317178601705606 1103 1 -0.12410996234875071 1103 2 -0.02708012564603024 1103 3 0.08900245942324148 1103 4 0.2124042563152102 1103 5 0.0714373790350109 1103 6 0.045369512626464825 1103 7 -0.06535149047189037 1103 8 0.20873695144831572 1103 9 0.02751095799076353 1103 10-11 -0.005150137417956557 1103 12-13 0.18245315723111588 1103 14-15 0.10047547512919408 1103 16-17 0.5657090323326415 1103 18-19 -0.03926931665494493 1103 20-21 0.22338695914869788 1103 22-23 0.31146473872590263 1103 24-25 0.10771466640832728 1103 26-27 0.23232005625622065 1103 28-29 0.13758561498725186 1103 30-31 0.2984329946724458 1103 32-33 0.5055932515018711 1103 34-35 0.8036722915934646 1103 36-37 1.0171435046714663 1103 38-39 0.6077355892827327 1103 40-41 -0.11740066872783217 1103 42-43 -0.20344146752119485 1103 44-45 -0.7800076942191794 1103 46-47 -0.5989681046211075 1103 48-49 0.012353037217870622 1103 50-51 0.4418020958014992 1103 52-53 0.2730068901025149 1103 54-55 -0.02720941709198854 1103 56-57 0.14174724543788386 1103 58-59 0.7826164808881408 1103 60-61 0.5615657775659422 1103 62-63 0.5256186957146518 1103 64-65 0.6370693072774252 1103 66-67 0.7330502041427884 1103 68-69 0.8193622431731598 1103 70-71 1.1148207912345391 1103 72-73 0.9590529931755505 1103 74-75 0.8466911567692286 1103 76-77 1.5220011772705533 1103 78-79 0.807261393138603 1103 80-81 0.8485265593312441 1103 82-83 1.119187860190511 1103 84-85 1.0877379160427907 1103 86-87 0.7254634657680334 1103 88-89 0.3945857708579119 1103 90-91 0.7002437519261839 1103 92-93 0.8001643736139457 1103 94-95 1.0151566868274102 1103 96-97 1.808645893766439 1103 98-99 1.2324186194349238 1103 100-101 0.9203497436426851 1104 1 0.09450443238415573 1104 2 0.34700336979015134 1104 3 0.1877605022183957 1104 4 0.16930983764095942 1104 5 0.08838336137826985 1104 6 0.30835020991809614 1104 7 0.030975009602926207 1104 8 0.39282343955245835 1104 9 0.3546843830992472 1104 10-11 0.10660730684359976 1104 12-13 0.3962418748728922 1104 14-15 -0.0462026634313375 1104 16-17 -0.19758963593010748 1104 18-19 0.20957102450920218 1104 20-21 0.2252573766258763 1104 22-23 0.37788326334059263 1104 24-25 0.4725395953324636 1104 26-27 0.4365135322400633 1104 28-29 0.43580497754897607 1104 30-31 0.4831554247188308 1104 32-33 0.44061494834461 1104 34-35 0.8151005423790494 1104 36-37 1.1045107050305845 1104 38-39 1.043393228067707 1104 40-41 0.5369461814891352 1104 42-43 0.14668198003230515 1104 44-45 0.30879972759736773 1104 46-47 0.6947996643449272 1104 48-49 1.1302828591541285 1104 50-51 1.0565147996769966 1104 52-53 1.128479731640752 1104 54-55 1.067415003078601 1104 56-57 0.7167696904476131 1104 58-59 0.9892976121166335 1104 60-61 0.922612463186173 1104 62-63 0.9860967744218208 1104 64-65 0.9882775969676842 1104 66-67 0.8240460144076387 1104 68-69 1.358192109999436 1104 70-71 1.249883637261771 1104 72-73 1.270458798951399 1104 74-75 1.102582971822347 1104 76-77 1.538685301167007 1104 78-79 1.299031556239008 1104 80-81 1.4436372880458954 1104 82-83 1.4620914962820848 1104 84-85 1.1270727955879032 1104 86-87 1.485227044998915 1104 88-89 1.1454461628974144 1104 90-91 1.9770842427237305 1104 92-93 2.0839441132518317 1104 94-95 1.9226458384060408 1104 96-97 2.3384416441779265 1104 98-99 2.646211077911655 1104 100-101 2.6518513147933653 1105 1 0.16081884069650698 1105 2 0.18806156334311197 1105 3 0.07236633930482128 1105 4 -0.21985289862628576 1105 5 -0.1647461822225722 1105 6 -0.10372114012977818 1105 7 -0.24055944197337453 1105 8 -0.20198488698394357 1105 9 -0.08210415550141192 1105 10-11 0.0298315346530984 1105 12-13 0.008302586245640953 1105 14-15 -0.16439834412923204 1105 16-17 0.08165785511567236 1105 18-19 0.16854120075658585 1105 20-21 0.019664979732375798 1105 22-23 0.06906025543251815 1105 24-25 -0.24025163839922925 1105 26-27 -0.2621991880691681 1105 28-29 -0.35656354615753827 1105 30-31 -0.32866414335095584 1105 32-33 -0.6197063396443312 1105 34-35 -0.25277420495242353 1105 36-37 0.0017233721464364748 1105 38-39 -0.02584681590548854 1105 40-41 0.5824442487964916 1105 42-43 0.38828629224195765 1105 44-45 -0.3885654271953527 1105 46-47 -0.20854797209607767 1105 48-49 0.15395391131909975 1105 50-51 0.20027876293810465 1105 52-53 0.264459730931506 1105 54-55 0.06057078874474797 1105 56-57 -0.14182458594516945 1105 58-59 0.14561521203152594 1105 60-61 -0.17632721552583774 1105 62-63 -0.17970697748427256 1105 64-65 -0.14456399315962187 1105 66-67 -0.029902707178230514 1105 68-69 0.1057008869064191 1105 70-71 -0.3455098164067998 1105 72-73 -0.05442860840347308 1105 74-75 0.31551605455577914 1105 76-77 0.4412173058480242 1105 78-79 0.36548781355309856 1105 80-81 -0.06781701884441915 1105 82-83 0.21433448362206065 1105 84-85 0.5200831860272821 1105 86-87 0.5603006291685695 1105 88-89 0.9927811747772672 1105 90-91 0.511659936897285 1105 92-93 0.5498471594591408 1105 94-95 1.126216887024789 1105 96-97 1.4259270159996262 1105 98-99 1.6397392172073744 1105 100-101 0.8763450911684139 1106 1 0.3932588477029846 1106 2 0.21549193643822662 1106 3 0.5247748171468203 1106 4 0.2731581365812872 1106 5 0.4243937722353053 1106 6 0.23406130863533292 1106 7 0.3000956639996559 1106 8 0.40263939047270725 1106 9 0.4194547865199567 1106 10-11 -0.04282567326422537 1106 12-13 -0.08631993959075857 1106 14-15 0.2211332726162638 1106 16-17 0.6712707470407011 1106 18-19 0.6603611341950213 1106 20-21 0.5669937588530587 1106 22-23 0.7731240144095679 1106 24-25 0.7988270093499352 1106 26-27 0.5518137739206708 1106 28-29 0.5278295174262766 1106 30-31 0.7553435637781334 1106 32-33 0.5879657570451116 1106 34-35 0.052988625665896905 1106 36-37 0.5914413612863925 1106 38-39 0.6409950127860569 1106 40-41 0.7907294125729791 1106 42-43 0.4961335509562659 1106 44-45 0.0019583072590165784 1106 46-47 -0.5528114157814912 1106 48-49 0.011004182820236963 1106 50-51 0.14666166712448359 1106 52-53 0.2582064466435412 1106 54-55 0.47325251934555723 1106 56-57 0.2767250724888797 1106 58-59 0.7451072421593956 1106 60-61 0.9139863244986834 1106 62-63 0.6282979270524223 1106 64-65 0.7537544514015941 1106 66-67 0.5280058582447822 1106 68-69 0.9991848595811419 1106 70-71 0.39048597970460364 1106 72-73 0.9817248335303184 1106 74-75 0.7545071212925087 1106 76-77 0.5176404973001176 1106 78-79 0.4123611714091169 1106 80-81 0.1788111121767777 1106 82-83 -0.6407714894029866 1106 84-85 0.24887982291640398 1106 86-87 0.34763641477024976 1106 88-89 0.3619176259946464 1106 90-91 0.18433466101709328 1106 92-93 -0.33472106614954455 1106 94-95 0.21563710072245712 1106 96-97 0.6367870615417495 1106 98-99 0.18727819075791174 1106 100-101 0.38256334305140527 1107 1 0.36049369618783444 1107 2 0.3598101182564051 1107 3 -0.12730851618650973 1107 4 -0.04445549978235164 1107 5 -0.05325774291620888 1107 6 -0.16423414591012175 1107 7 0.1896789973329831 1107 8 -0.03486060952729275 1107 9 -0.05800733469216368 1107 10-11 0.27526144794789786 1107 12-13 0.39114218162136183 1107 14-15 -0.0034879395049571826 1107 16-17 -0.02285804083808074 1107 18-19 -0.17836992641104388 1107 20-21 -0.3346160896317869 1107 22-23 -0.13975477346922105 1107 24-25 0.1693762517741817 1107 26-27 0.030033470890366232 1107 28-29 0.059079517426276595 1107 30-31 0.29492689711147335 1107 32-33 -0.056068333863983355 1107 34-35 0.028367413544692965 1107 36-37 -0.10808515386512596 1107 38-39 -0.7794595326684899 1107 40-41 -0.5696872540936866 1107 42-43 -0.5798134187406987 1107 44-45 -0.8681174503167384 1107 46-47 -1.3018644460845223 1107 48-49 -1.428957938391882 1107 50-51 -1.900118635905823 1107 52-53 -2.0034412806291897 1107 54-55 -2.135364904896864 1107 56-57 -2.4431612911474794 1107 58-59 -2.4189079093557595 1107 60-61 -2.2359189785316183 1107 62-63 -2.0354331335536386 1107 64-65 -1.8567569122347685 1107 66-67 -2.0930168690279416 1107 68-69 -2.7474060495097667 1107 70-71 -2.3691731112044856 1107 72-73 -2.4032183482878615 1107 74-75 -2.360549696889308 1107 76-77 -2.0693860178514 1107 78-79 -2.4419001922272514 1107 80-81 -2.4138972211565566 1107 82-83 -2.361604822736318 1107 84-85 -1.8988474498108658 1107 86-87 -2.253499948866118 1107 88-89 -1.9112831315811114 1107 90-91 -1.5435062480738146 1107 92-93 -1.2340581873616685 1107 94-95 -0.8399500204896633 1107 96-97 -2.0605614233067335 1107 98-99 -1.2220021122723956 1107 100-101 -1.0925313539182895 1108 1 0.11447096891510711 1108 2 -0.3057201847738966 1108 3 0.05204754441955117 1108 4 -0.2844176209944749 1108 5 -0.38166683382529953 1108 6 -0.159878085304058 1108 7 -0.5605103966064107 1108 8 -0.44584545801214404 1108 9 -0.30175733469216226 1108 10-11 -0.2185832490218047 1108 12-13 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-0.6632440433543039 2316 88-89 -1.1885715174078832 2316 90-91 -0.9050712087037383 2316 92-93 -0.37719302988135084 2316 94-95 -0.7520317134030492 2316 96-97 -1.22916378551146 2316 98-99 -0.2905552618786942 2316 100-101 -0.20810714131986785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 1.0 5 4.0 6 22.0 7 59.0 8 118.0 9 182.0 10 316.0 11 520.0 12 807.0 13 1065.0 14 1419.0 15 1993.0 16 2795.0 17 3789.0 18 3065.0 19 1695.0 20 1397.0 21 1332.0 22 1422.0 23 1422.0 24 1704.0 25 1887.0 26 2057.0 27 2505.0 28 2972.0 29 3533.0 30 4377.0 31 5313.0 32 6651.0 33 8425.0 34 11909.0 35 17460.0 36 19920.0 37 23790.0 38 12935.0 39 636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.3816207609401 22.37862356412195 17.065289383366895 14.174466291571052 2 15.215282840917451 27.73626932261323 35.213142387007444 21.835305449461874 3 25.144982307810647 27.161691226145646 34.104788596731794 13.588537869311912 4 10.987886368653053 20.73096501026749 40.799058200279596 27.48209042079986 5 8.310914809609852 43.906846014856455 35.00568828213879 12.7765508933949 6 22.86406110995913 41.209757794262245 16.528986428184428 19.3971946675942 7 20.415248058541412 40.857251790957925 21.971090494377965 16.7564096561227 8 24.326927579079737 29.18442017110253 20.217924963712615 26.27072728610511 9 21.474772743995025 22.45135484043585 26.471394840168294 29.602477575400833 10-11 24.12793225463375 27.852322058046436 26.20149697995331 21.818248707366507 12-13 29.371176673233286 28.418626896821603 26.285423780967065 15.92477264897805 14-15 21.98948502016709 30.694777961351427 32.72586805439428 14.589868964087197 16-17 15.268125296820756 32.873358706630725 32.77436271329289 19.08415328325563 18-19 14.59187563962783 33.71683132554297 35.58872515902904 16.102567875800162 20-21 18.2858308640076 27.63058441080662 34.58973518571782 19.493849539467963 22-23 16.573133290078328 28.47104701640792 36.67199550504679 18.283824188466966 24-25 20.712570484478366 29.263014963110617 34.837225169062414 15.187189383348606 26-27 17.965826201250163 26.735362089090003 41.05839110905984 14.240420600599998 28-29 14.519683880655917 34.31593874267979 36.49318898599002 14.671188390674278 30-31 16.658082554631743 28.643955558825695 36.04123049344152 18.65673139310105 32-33 16.648049176928584 30.315516284172013 37.594731807814 15.44170273108541 34-35 19.39686022167076 28.71753366198219 36.045578290446215 15.84002782590083 36-37 17.340686684370006 27.774061711961796 39.39906756476545 15.486184038902751 38-39 15.774141978983419 31.024876087785362 37.87967973458372 15.321302198647501 40-41 15.858528428093646 31.873913043478264 36.08963210702341 16.17792642140468 42-43 15.255836045504262 31.723535845812666 33.369904435695624 19.650723672987446 44-45 17.894194687660953 28.280078394124452 34.15462103932415 19.67110587889044 46-47 20.852034434552277 31.475709192580652 34.01549153517367 13.656764837693395 48-49 18.984822843994355 30.172038983016837 34.45160901933767 16.391529153651145 50-51 13.779840937518811 32.18339676657681 37.33286064976154 16.703901646142835 52-53 17.63633688068976 34.65160768155397 30.26033270680464 17.451722730951634 54-55 15.957083899104354 30.437254600303675 32.52620383810142 21.079457662490555 56-57 19.716256078554657 30.034247262560115 31.31316847378947 18.936328185095753 58-59 22.938642550885945 29.676055678557333 30.669025625246654 16.716276145310065 60-61 18.240346218419944 31.77202828074729 35.225516886174674 14.762108614658095 62-63 16.16510926348319 31.35397087644899 34.62150754844449 17.859412311623334 64-65 15.664443716095544 35.1893967264433 32.0101537782356 17.136005779225556 66-67 18.19385823506197 30.9325690129163 29.560337389047564 21.31323536297416 68-69 16.394873612885533 30.78006167182828 32.20814576491127 20.616918950374913 70-71 20.915579160005617 30.96902361857111 30.44661908615996 17.66877813526331 72-73 18.923284794081646 27.958341415776484 36.023839305422705 17.094534484719166 74-75 15.71260392907071 35.45260566818951 31.24527595133143 17.589514451408352 76-77 17.039350907351793 30.72287141892027 31.09544417763092 21.142333496097017 78-79 17.2878442284667 31.487749245824443 32.48640477321222 18.73800175249664 80-81 19.589501073571412 29.600136453936766 31.51784937893392 19.292513093557904 82-83 17.167166664994433 29.4407042116916 34.7560710499296 18.63605807338437 84-85 16.228988434859968 31.15664778162019 33.406465508591914 19.207898274927928 86-87 15.92163263121986 32.34694082313831 31.65731332900783 20.074113216634 88-89 15.503240780998121 32.068681814837355 29.663346733466668 22.764730670697855 90-91 17.4903836505335 27.75328628290464 31.529584908184766 23.226745158377092 92-93 19.139720932721506 30.631513465063076 32.125513384794445 18.103252217420966 94-95 17.698878268372788 29.25398492317777 32.80245617086173 20.24468063758771 96-97 13.606932395101037 30.21250693975291 35.362639714784514 20.817920950361536 98-99 16.702341137123746 31.601672240802674 29.694648829431436 22.00133779264214 100-101 15.091202847308406 28.258666193899295 32.50888947613138 24.14124148266092 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3446.0 1 2045.0 2 666.0 3 628.5 4 451.0 5 460.5 6 847.0 7 1515.0 8 1902.5 9 1787.0 10 1354.5 11 938.5 12 836.0 13 781.0 14 705.0 15 648.0 16 639.5 17 654.0 18 720.5 19 781.0 20 828.0 21 898.5 22 958.5 23 1040.0 24 1134.0 25 1312.5 26 1717.5 27 2287.5 28 2463.5 29 2540.0 30 2725.5 31 2873.0 32 3278.5 33 3591.0 34 3866.0 35 4212.5 36 4688.5 37 5029.5 38 4508.5 39 3642.0 40 3162.5 41 2853.0 42 2477.5 43 2344.0 44 2374.0 45 2274.0 46 2735.5 47 7941.0 48 17249.0 49 15880.5 50 6742.5 51 2601.5 52 1294.5 53 870.5 54 691.5 55 599.0 56 541.0 57 495.0 58 510.5 59 486.5 60 446.0 61 399.5 62 299.0 63 183.5 64 93.0 65 74.5 66 53.0 67 29.5 68 33.5 69 30.5 70 14.5 71 7.5 72 8.5 73 7.0 74 5.0 75 3.5 76 3.0 77 2.5 78 0.5 79 1.5 80 2.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018728971712563797 2 0.0 3 0.0 4 0.0 5 0.047491321128286766 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005016688851579588 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.344459234386392E-4 28-29 3.344459234386392E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.688918468772784E-4 42-43 0.013712282860984209 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.344459234386392E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010702269550036454 92-93 0.002006675540631835 94-95 0.0 96-97 0.0 98-99 6.688918468772784E-4 100-101 0.017725633942247878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 149501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.076715205918354 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12742143779595 24.27943625795145 2 10.333620318553596 6.422699513715627 3 3.6762806715454155 3.4274018233991748 4 1.9306930693069306 2.399983946595675 5 1.175204476969436 1.82607474197497 6 0.699526474386569 1.3043391014106929 7 0.6306500215238915 1.371897177945298 8 0.4175634954799828 1.038120146353536 9 0.2884201463624623 0.8066835673339977 >10 2.3740852346104178 13.879505822703528 >50 0.16573396470081792 3.550477923224594 >100 0.12268618166164443 7.159149437127511 >500 0.017219113215669393 3.87355268526632 >1k 0.03874300473525613 24.104186594069603 >5k 0.002152389151958674 4.556491260928021 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6812 4.556491260928021 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3257 2.178580745279296 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3073 2.0555046454538766 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 1.82607474197497 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 1.823399174587461 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2717 1.8173791479655654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 1.6394539166962092 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2164 1.4474819566424304 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1998 1.3364459100608022 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1856 1.2414632678042288 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1801 1.2046742162259785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1778 1.189289703747801 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1592 1.0648758202286273 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1590 1.0635380365348728 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1454 0.9725687453595628 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1408 0.941799720403208 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1292 0.8642082661654437 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1099 0.735112139718129 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1050 0.7023364392211424 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 964 0.6448117403896965 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 923 0.617387174667728 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 918 0.6140427154333417 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 700 0.46822429281409494 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.4173885124514217 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 589 0.393977297810717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.3752483260981532 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 512 0.34247262560116654 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 485 0.32441254573548006 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 453 0.3030080066354071 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 0.29297462893224796 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.25484779366024307 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 360 0.24080106487582026 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 331 0.22140320131637917 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 316 0.21136982361322 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 313 0.20936314807258813 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.20401201329756993 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 287 0.1919719600537789 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.1739118801880924 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 260 0.1739118801880924 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 243 0.16254071879117865 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 239 0.15986515140366953 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 234 0.15652069216928313 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 228 0.15250734108801947 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.14581842261924668 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 211 0.14113617969110573 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 192 0.12842723460043745 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.12240720797854195 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 182 0.12173831613166468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 169 0.11304272212226006 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 167 0.11170493842850548 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 160 0.10702269550036454 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 158 0.10568491180661 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 152 0.10167156072534633 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 151 0.10100266887846904 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.344459234386392E-4 0.0 16-17 0.0 0.0 0.0 0.0013377836937545568 0.0 18-19 0.0 0.0 0.0 0.0013377836937545568 0.0 20-21 0.0 0.0 0.0 0.0013377836937545568 0.0 22-23 0.0 0.0 0.0 0.0013377836937545568 0.0 24-25 0.0 0.0 0.0 0.003344459234386392 0.0 26-27 0.0 0.0 0.0 0.006354472545334145 0.0 28-29 0.0 0.0 0.0 0.01337783693754557 0.0 30-31 0.0 0.0 0.0 0.039799064889198064 0.0 32-33 0.0 0.0 0.0 0.06521695507053464 0.0 34-35 0.0 0.0 0.0 0.09431375040969625 0.0 36-37 0.0 0.0 0.0 0.12173831613166466 0.0 38-39 6.688918468772784E-4 0.0 0.0 0.17023297503026735 0.0 40-41 6.688918468772784E-4 0.0 0.0 0.26722229282747273 0.0 42-43 6.688918468772784E-4 0.0 0.0 0.35284044922776436 0.0 44-45 6.688918468772784E-4 0.0 0.0 0.45016421294840836 0.0 46-47 6.688918468772784E-4 0.0 0.0 0.5973204192614097 0.0 48-49 6.688918468772784E-4 0.0 0.0 0.6949786289054922 0.0 50-51 6.688918468772784E-4 0.0 0.0 0.8367837004434753 0.0 52-53 6.688918468772784E-4 0.0 0.0 0.9718998535126855 0.0 54-55 6.688918468772784E-4 0.0 0.0 1.123403856830389 0.0 56-57 6.688918468772784E-4 0.0 0.0 1.4427997137142894 0.0 58-59 6.688918468772784E-4 0.0 0.0 1.667547374265055 0.0 60-61 6.688918468772784E-4 0.0 0.0 1.8692182660985543 0.0 62-63 6.688918468772784E-4 0.0 0.0 2.0862736704102316 0.0 64-65 6.688918468772784E-4 0.0 0.0 2.2976434940234514 0.0 66-67 6.688918468772784E-4 0.0 0.0 2.6264038367636338 0.0 68-69 6.688918468772784E-4 0.0 0.0 3.028407836736878 0.0 70-71 6.688918468772784E-4 0.0 0.0 3.365863773486465 0.0 72-73 6.688918468772784E-4 0.0 0.0 3.6906107651453834 0.0 74-75 6.688918468772784E-4 0.0 0.0 4.1748884622845335 0.0 76-77 6.688918468772784E-4 0.0 0.0 4.599300339128167 0.0 78-79 6.688918468772784E-4 0.0 0.0 5.2340787018147035 0.0 80-81 6.688918468772784E-4 0.0 0.0 5.847118079477729 0.0 82-83 6.688918468772784E-4 0.0 0.0 6.373870408893586 0.0 84-85 6.688918468772784E-4 0.0 0.0 7.070521267416272 0.0 86-87 6.688918468772784E-4 0.0 0.0 7.637407107644765 0.0 88-89 6.688918468772784E-4 0.0 0.0 8.372519247362895 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 15 6.573427E-4 95.120224 1 CGTATCA 15 6.5909536E-4 95.05655 2 GTACATA 25 0.0049877134 57.07214 1 CTCTAAT 25 0.0049877134 57.07214 1 CAGGGGC 25 0.005007587 57.014854 9 TTAGGCA 50 2.8052205E-5 47.528282 4 GGGAACC 40 5.366157E-4 47.51238 9 GTAAGCG 35 7.878407E-8 47.51238 94-95 GGCTAGT 45 9.538588E-4 42.275658 1 GTAGTTC 45 9.601679E-4 42.2191 6 ATGGGTG 50 0.0016066141 38.022625 5 AGTAGTT 50 0.0016066141 38.022625 5 TGGGTGA 50 0.0016119075 37.997192 6 GCTAGTA 55 0.00256498 34.566025 2 CATTCCG 55 0.002569195 34.55446 9 GGCATTC 55 0.002569195 34.55446 7 TGGGAAC 55 0.002569195 34.55446 8 CTAGTAG 60 0.0039285915 31.685516 3 CTTTAGG 60 0.0039285915 31.685516 2 AGTTCTC 60 0.0039350362 31.674917 8 >>END_MODULE