##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937730_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141673 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67631799990118 33.0 31.0 34.0 30.0 34.0 2 31.731847282121507 33.0 31.0 34.0 30.0 34.0 3 31.701721570094513 33.0 31.0 34.0 30.0 34.0 4 35.317039944096614 37.0 35.0 37.0 33.0 37.0 5 35.33336627303721 37.0 35.0 37.0 33.0 37.0 6 35.515179321395046 37.0 35.0 37.0 33.0 37.0 7 35.494871993957915 37.0 35.0 37.0 33.0 37.0 8 35.53742773852463 37.0 35.0 37.0 33.0 37.0 9 37.04957895999944 39.0 37.0 39.0 34.0 39.0 10-11 36.96863551982382 39.0 37.0 39.0 33.5 39.0 12-13 36.89575289575289 39.0 37.0 39.0 33.0 39.0 14-15 37.82280321585623 40.0 38.0 41.0 33.0 41.0 16-17 37.83569205141417 40.0 37.5 41.0 33.0 41.0 18-19 37.89086840823587 40.0 37.5 41.0 33.5 41.0 20-21 37.814209482399605 40.0 37.0 41.0 33.0 41.0 22-23 37.68453057392728 40.0 37.0 41.0 33.0 41.0 24-25 37.44809879087758 39.5 36.5 41.0 32.5 41.0 26-27 37.426789155308356 39.5 36.5 41.0 33.0 41.0 28-29 37.3600650794435 39.0 36.0 41.0 33.0 41.0 30-31 37.103738891673075 39.0 35.5 41.0 33.0 41.0 32-33 36.61920761189499 38.5 35.0 40.5 32.0 41.0 34-35 36.24375145581727 38.0 35.0 40.0 31.0 41.0 36-37 35.98764055254 38.0 35.0 40.0 30.5 41.0 38-39 35.787171867610624 38.0 35.0 40.0 30.0 41.0 40-41 35.618579404685434 38.0 35.0 40.0 30.0 41.0 42-43 35.183923542241644 38.0 35.0 40.0 28.0 41.0 44-45 34.72081130490636 38.0 35.0 40.0 24.5 41.0 46-47 33.98280547457878 38.0 34.5 40.0 19.0 41.0 48-49 33.314393003606895 38.0 33.0 40.0 6.5 41.0 50-51 32.2587296097351 37.0 32.0 39.5 2.0 40.5 52-53 32.43868627049614 37.5 33.0 39.5 2.0 40.5 54-55 32.77643587698432 38.0 33.0 40.0 2.0 41.0 56-57 32.41962124046219 38.0 33.0 40.0 2.0 41.0 58-59 32.1890303727598 37.0 33.0 40.0 2.0 41.0 60-61 31.746010178368497 36.5 32.0 40.0 2.0 41.0 62-63 31.44339429531386 36.0 31.0 39.5 2.0 41.0 64-65 31.10853161858646 36.0 31.0 39.0 2.0 40.5 66-67 30.52764111722064 35.0 30.5 39.0 2.0 40.0 68-69 29.986317082295145 35.0 29.5 38.0 2.0 40.0 70-71 29.32155738919907 35.0 29.0 37.0 2.0 39.0 72-73 28.81094845171628 35.0 28.0 37.0 2.0 39.0 74-75 28.36117679444919 34.0 27.0 36.0 2.0 38.5 76-77 27.92915022622518 34.0 27.0 36.0 2.0 37.5 78-79 27.33606615233672 34.0 26.0 35.0 2.0 37.0 80-81 26.727347483288984 33.5 24.5 35.0 2.0 36.5 82-83 26.171451158654087 33.0 22.0 35.0 2.0 36.0 84-85 25.644671179406096 33.0 20.0 35.0 2.0 35.5 86-87 25.298903107861058 33.0 19.0 35.0 2.0 35.0 88-89 24.659539926450346 32.0 9.5 35.0 2.0 35.0 90-91 24.067165232613128 32.0 2.0 35.0 2.0 35.0 92-93 23.47579284690802 31.0 2.0 34.5 2.0 35.0 94-95 22.776739392827146 31.0 2.0 34.0 2.0 35.0 96-97 22.021052000028234 30.5 2.0 34.0 2.0 35.0 98-99 21.20940828527666 30.0 2.0 34.0 2.0 35.0 100-101 19.111891468381415 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26833938273552604 1101 2 -0.014785198805970623 1101 3 0.0912419081953253 1101 4 -0.4088178371563842 1101 5 -0.20094108428786228 1101 6 0.026221263976061948 1101 7 0.1693570680319354 1101 8 0.06013475841495364 1101 9 0.28445580119457503 1101 10-11 0.12667002770590585 1101 12-13 0.0201952026872263 1101 14-15 -0.05154237599481348 1101 16-17 0.2582156160354714 1101 18-19 0.5468330068978915 1101 20-21 0.7747363476717908 1101 22-23 0.5176129122253172 1101 24-25 0.4297335569256546 1101 26-27 0.5408107351909592 1101 28-29 0.5671262067007561 1101 30-31 0.5552737885631913 1101 32-33 0.5129651305305032 1101 34-35 0.12025879962000374 1101 36-37 -0.13394150148255335 1101 38-39 -0.3081707449782485 1101 40-41 -0.751435905174624 1101 42-43 -1.2158343032698227 1101 44-45 -1.1212251280889802 1101 46-47 -1.0983224573517916 1101 48-49 -0.8205954157856539 1101 50-51 -0.6381976138108953 1101 52-53 -0.49576028967849695 1101 54-55 -0.5332105288996303 1101 56-57 -0.555199867406877 1101 58-59 -0.6222563700385564 1101 60-61 -0.4818926989299719 1101 62-63 -0.342506489697751 1101 64-65 -0.8175184861239764 1101 66-67 -0.6299483872211624 1101 68-69 -0.41905715121186304 1101 70-71 -0.4825621586851305 1101 72-73 -0.40472719411753744 1101 74-75 -0.5218519701321007 1101 76-77 -1.1886837213591974 1101 78-79 -0.9306820994332874 1101 80-81 -0.9728370119835859 1101 82-83 -0.6483705508598945 1101 84-85 -0.7793165191017124 1101 86-87 -1.008577913842693 1101 88-89 -0.053313653093251645 1101 90-91 -0.09621400969160732 1101 92-93 0.6021642466287247 1101 94-95 0.622520840234781 1101 96-97 0.9373500213330139 1101 98-99 1.4218277865678637 1101 100-101 1.5507192154392335 1102 1 -0.09882666342014446 1102 2 0.026674689435989052 1102 3 -0.2681813265356361 1102 4 -0.42183022432374884 1102 5 -0.41824404235906343 1102 6 -0.30518430829543775 1102 7 0.07748883310559052 1102 8 -0.17143102643128572 1102 9 -0.3650694225168891 1102 10-11 0.1174871118370504 1102 12-13 0.01769432347187916 1102 14-15 -0.06410538642816732 1102 16-17 0.551893080769176 1102 18-19 -0.023386992320588718 1102 20-21 -0.05121808943499673 1102 22-23 0.2218839450102763 1102 24-25 -0.1860297973785876 1102 26-27 0.09082831949794468 1102 28-29 -0.10656767005279022 1102 30-31 -0.21663821174941944 1102 32-33 0.025254607299686427 1102 34-35 0.17117513739500367 1102 36-37 0.5837912952037811 1102 38-39 0.7701380354523621 1102 40-41 1.174299705000827 1102 42-43 1.6169389373225656 1102 44-45 1.5238649426390083 1102 46-47 1.8363420731472146 1102 48-49 1.5140339460993815 1102 50-51 1.3319090643181326 1102 52-53 1.2530967303676093 1102 54-55 1.433262059119592 1102 56-57 1.6920941031296408 1102 58-59 1.7115719289728126 1102 60-61 1.9358713587465566 1102 62-63 2.1562626088134422 1102 64-65 2.125371592419267 1102 66-67 2.0102376156704764 1102 68-69 1.4499397617653536 1102 70-71 2.0164023210147626 1102 72-73 1.7165849859457651 1102 74-75 2.0275990087276554 1102 76-77 1.5141219525105214 1102 78-79 1.3464856548553321 1102 80-81 1.291201126424447 1102 82-83 1.8974658760089227 1102 84-85 1.3107151326508557 1102 86-87 1.4892924312004823 1102 88-89 1.1796393381927466 1102 90-91 1.9216242928366256 1102 92-93 1.9425964298181242 1102 94-95 2.2204654301296642 1102 96-97 2.6406636862933475 1102 98-99 2.9893906016200305 1102 100-101 2.5060941519403492 1103 1 0.37225911724562977 1103 2 0.35513150869035925 1103 3 0.35057933705749633 1103 4 0.554247025467518 1103 5 0.48639167114875903 1103 6 0.39285903580736914 1103 7 0.14007278054088346 1103 8 0.32350490125594433 1103 9 0.4338923519050084 1103 10-11 0.277595290586099 1103 12-13 0.3112290096445989 1103 14-15 0.5011467281396662 1103 16-17 0.5318182994862752 1103 18-19 0.5918524166894699 1103 20-21 0.6725993861521786 1103 22-23 0.39685762635488686 1103 24-25 0.22713679063462422 1103 26-27 0.2704304540316542 1103 28-29 0.7942019237323734 1103 30-31 0.8887453145873891 1103 32-33 1.13134601471247 1103 34-35 1.0291270014818394 1103 36-37 1.351152532362093 1103 38-39 0.7689219320654246 1103 40-41 0.9928824907851279 1103 42-43 0.7101069773995334 1103 44-45 0.7740373752088061 1103 46-47 0.9886635600604805 1103 48-49 2.156263589980746 1103 50-51 1.9136058008048593 1103 52-53 2.0345792874518693 1103 54-55 1.9849555284628977 1103 56-57 1.8064441230955168 1103 58-59 1.8589745499478774 1103 60-61 1.8210131164697252 1103 62-63 1.817841002104906 1103 64-65 1.5782339730777757 1103 66-67 1.5872151449840146 1103 68-69 1.3637416276672667 1103 70-71 1.4592273110318246 1103 72-73 1.1961471887264707 1103 74-75 1.2510534683784336 1103 76-77 1.3504562175847603 1103 78-79 1.5773456396086623 1103 80-81 0.9388578585549858 1103 82-83 1.0672665802598402 1103 84-85 0.8825196848707009 1103 86-87 1.5021068937553892 1103 88-89 1.588358980985067 1103 90-91 1.5210362070196624 1103 92-93 1.6878499964496996 1103 94-95 1.684236439676983 1103 96-97 2.3206651383570964 1103 98-99 1.8568897848341734 1103 100-101 1.5681880278615061 1104 1 0.16808145987056733 1104 2 0.2300156606829411 1104 3 0.4473926257150822 1104 4 0.5449078937928888 1104 5 0.31333257084221344 1104 6 0.1435184783111083 1104 7 0.3262137943000667 1104 8 0.460027322301066 1104 9 1.0024743722942588 1104 10-11 0.6553132630677325 1104 12-13 0.49843938962606416 1104 14-15 0.7447127080355713 1104 16-17 0.7223971830679403 1104 18-19 0.5337680080489875 1104 20-21 0.3985564689211287 1104 22-23 0.699506090032088 1104 24-25 0.772780905555841 1104 26-27 0.9036378397876916 1104 28-29 0.8444264906390586 1104 30-31 1.1412226796690206 1104 32-33 1.3849818598682404 1104 34-35 1.3277831645958358 1104 36-37 1.6626589129138836 1104 38-39 1.58729007921022 1104 40-41 1.6768313037886244 1104 42-43 1.521292548084638 1104 44-45 2.0051274158447114 1104 46-47 2.832553914377165 1104 48-49 2.4990845780751414 1104 50-51 2.3087366199393955 1104 52-53 2.447586345268938 1104 54-55 2.3451180721286917 1104 56-57 2.446085528955642 1104 58-59 2.2007618173607177 1104 60-61 2.28403086794901 1104 62-63 2.166222694555188 1104 64-65 2.3528828645014563 1104 66-67 2.5870725628210423 1104 68-69 2.3684111935045813 1104 70-71 2.0445270900294723 1104 72-73 1.8447356253130494 1104 74-75 1.8868273330679592 1104 76-77 1.9232800573224083 1104 78-79 1.8480378760098901 1104 80-81 1.3678697641655937 1104 82-83 1.8408282826908682 1104 84-85 1.9195554017025245 1104 86-87 2.0199118413564463 1104 88-89 2.43889722865028 1104 90-91 2.9586743334900376 1104 92-93 2.449969480302272 1104 94-95 2.9791319982426074 1104 96-97 2.8241584013498944 1104 98-99 3.31049711355735 1104 100-101 3.120533504442381 1105 1 0.3367561586657466 1105 2 0.43674501713313063 1105 3 0.21715460044326562 1105 4 0.3108497098375551 1105 5 0.5094830263226697 1105 6 0.4010853310880691 1105 7 0.5984001005004771 1105 8 0.49690654651499955 1105 9 0.5516221830494743 1105 10-11 0.7267943092035054 1105 12-13 0.609774975873492 1105 14-15 0.8703738893461832 1105 16-17 0.8756810502439549 1105 18-19 0.8922384752837829 1105 20-21 1.1513481233436949 1105 22-23 1.1645369451599095 1105 24-25 1.2817803178391287 1105 26-27 1.3528738080509868 1105 28-29 1.4317758882294171 1105 30-31 1.1439408695015203 1105 32-33 1.0246747778817564 1105 34-35 1.0471851628326831 1105 36-37 0.9711734589027259 1105 38-39 1.060071448590179 1105 40-41 1.2798286590633765 1105 42-43 1.4850745051813234 1105 44-45 1.1059575657124796 1105 46-47 0.6744948488467486 1105 48-49 1.242729891034287 1105 50-51 0.7295785241415658 1105 52-53 0.9823350963709103 1105 54-55 1.0285224212324167 1105 56-57 1.1903920232254066 1105 58-59 1.357314858794755 1105 60-61 1.1864698923392574 1105 62-63 1.4472247515637093 1105 64-65 1.9673774281217895 1105 66-67 1.691649406782254 1105 68-69 1.515928090360294 1105 70-71 1.9303631170644415 1105 72-73 1.9894976000412328 1105 74-75 1.490265475883124 1105 76-77 1.5434313943779472 1105 78-79 2.2616435180904055 1105 80-81 2.140239731841177 1105 82-83 2.3011206717593353 1105 84-85 2.105567743753653 1105 86-87 2.1723875980417233 1105 88-89 2.4297141548918333 1105 90-91 2.47884036346359 1105 92-93 2.807888963111555 1105 94-95 2.804690329127123 1105 96-97 2.8885501145441346 1105 98-99 2.5692761320704243 1105 100-101 3.5406407627438803 1106 1 0.30645312836271543 1106 2 -0.26118550762666004 1106 3 -0.04278627169273008 1106 4 -0.0017149612637012979 1106 5 -0.03464114219174519 1106 6 -0.1072664797027656 1106 7 0.07057304950269128 1106 8 0.048052148880110224 1106 9 0.17235389036655135 1106 10-11 0.12265535872308675 1106 12-13 -0.07804837963278999 1106 14-15 -0.11556476549491634 1106 16-17 -0.18324356172943368 1106 18-19 0.12403817964446517 1106 20-21 0.16054989717961377 1106 22-23 -0.2648422122680216 1106 24-25 -0.06214059864276322 1106 26-27 0.2935611694700597 1106 28-29 0.7211698276233562 1106 30-31 0.26681596188880263 1106 32-33 0.44834440094162176 1106 34-35 -0.023786751431913444 1106 36-37 0.011435838799243925 1106 38-39 0.31618748702329214 1106 40-41 -0.10380031358998565 1106 42-43 -0.33004005505519274 1106 44-45 -0.28567214603918245 1106 46-47 0.09036698484083416 1106 48-49 -0.03437653912092031 1106 50-51 -0.6624207367601329 1106 52-53 -0.4518112450925109 1106 54-55 -0.3894930998318813 1106 56-57 -0.29613791025575154 1106 58-59 -0.2896178832599432 1106 60-61 -0.1659691320509893 1106 62-63 -0.2562490104466413 1106 64-65 -8.228675175274702E-4 1106 66-67 0.40116529739570694 1106 68-69 0.672025651335904 1106 70-71 0.9029425701317031 1106 72-73 0.09243551578402531 1106 74-75 0.4897481070730727 1106 76-77 0.7117795096772817 1106 78-79 0.6929812860135414 1106 80-81 0.2850475662831542 1106 82-83 0.7513978336218656 1106 84-85 1.4362403232067216 1106 86-87 1.210839187103069 1106 88-89 1.5797880647218427 1106 90-91 1.402029572628411 1106 92-93 1.6293597687287047 1106 94-95 1.6878943202579428 1106 96-97 1.6819167073009744 1106 98-99 2.1118297166971836 1106 100-101 1.9202231722043415 1107 1 -0.005709594758911862 1107 2 0.35355089752070157 1107 3 -0.09774683624233305 1107 4 0.28562418360969133 1107 5 0.5098171392528386 1107 6 0.35865298895642894 1107 7 0.21926545298961742 1107 8 -0.08461700844657827 1107 9 -0.09613926031838105 1107 10-11 0.2561962470584973 1107 12-13 0.2901541940492365 1107 14-15 0.1562492652232308 1107 16-17 0.25585980066159664 1107 18-19 0.354943119453516 1107 20-21 0.2586819021609301 1107 22-23 0.4779348736597484 1107 24-25 0.3978427969570717 1107 26-27 0.39848022301924857 1107 28-29 0.3941046553527059 1107 30-31 0.47997494902039506 1107 32-33 0.3856420348145946 1107 34-35 0.8470932817768855 1107 36-37 0.8747401144322922 1107 38-39 0.9630534064919445 1107 40-41 0.20707556851877484 1107 42-43 -0.24512930370608643 1107 44-45 -0.3245887089283457 1107 46-47 0.26942468513141193 1107 48-49 0.06811412090398505 1107 50-51 0.33052239316929644 1107 52-53 0.10161341244173627 1107 54-55 -0.17691526670610358 1107 56-57 -0.11101960390456611 1107 58-59 0.4456042005922747 1107 60-61 0.6675925117846297 1107 62-63 0.6993964856098103 1107 64-65 0.5602190585928923 1107 66-67 1.1179357415634108 1107 68-69 1.3713407198290533 1107 70-71 0.8751509553537851 1107 72-73 0.820144108561113 1107 74-75 0.4117489372930798 1107 76-77 0.6325142543846276 1107 78-79 1.0214163212978882 1107 80-81 1.1568200860008808 1107 82-83 1.6242496393503814 1107 84-85 1.4679132165234492 1107 86-87 1.1230641767253182 1107 88-89 0.7912243453362038 1107 90-91 0.5756078208641924 1107 92-93 1.532078739255894 1107 94-95 1.2011570848905713 1107 96-97 1.1604762755865679 1107 98-99 1.2481518420604054 1107 100-101 1.4970807894729141 1108 1 0.09147313979782368 1108 2 0.08952918648425978 1108 3 0.4464075533501557 1108 4 0.5678676573755226 1108 5 0.3642153472307825 1108 6 0.25888560720404286 1108 7 0.4063077550487151 1108 8 0.39190577952619776 1108 9 0.44405200357409313 1108 10-11 0.5973265994320656 1108 12-13 0.7101162858903649 1108 14-15 0.4420681676097189 1108 16-17 0.48959806205558465 1108 18-19 0.37818340548780327 1108 20-21 0.47152759872335537 1108 22-23 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-4.8403530439708256 2316 74-75 -4.291729591258559 2316 76-77 -4.583623963254279 2316 78-79 -4.415939381334095 2316 80-81 -5.258602416692668 2316 82-83 -4.582922901822464 2316 84-85 -4.940198219523957 2316 86-87 -5.370740656274528 2316 88-89 -5.263146902932231 2316 90-91 -4.925277202992973 2316 92-93 -5.4696528291602995 2316 94-95 -6.281257875860547 2316 96-97 -6.087984552487928 2316 98-99 -5.527359932604828 2316 100-101 -5.28893583358388 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 898.0 3 158.0 4 109.0 5 113.0 6 128.0 7 154.0 8 179.0 9 239.0 10 295.0 11 492.0 12 752.0 13 1153.0 14 1390.0 15 1848.0 16 2422.0 17 3379.0 18 2676.0 19 1494.0 20 1367.0 21 1370.0 22 1448.0 23 1563.0 24 1786.0 25 1870.0 26 2048.0 27 2354.0 28 2755.0 29 3175.0 30 3785.0 31 4684.0 32 6057.0 33 7552.0 34 10802.0 35 15950.0 36 17590.0 37 21654.0 38 14545.0 39 1439.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.069699567167035 21.522636951609133 16.288970905973947 15.11869257524989 2 14.748746565447696 25.817919157012152 35.88080333116166 23.552530946378493 3 23.72907757229035 27.33297459571214 34.3333144524059 14.604633379591606 4 10.404231365635024 19.87099149620126 41.773406688333296 27.95137044983042 5 8.255204645234386 42.68942076193174 35.41070669876788 13.644667894065995 6 22.841598098243278 39.62417664829528 17.135620441054737 20.3986048124067 7 19.409858796230793 40.40649141175472 22.959053736664874 17.224596055349615 8 23.14369393587821 28.58173689125329 21.24281954780837 27.03174962506013 9 21.747405211509754 21.305212217261797 27.73222207285925 29.21516049836919 10-11 23.820203256490164 27.056530490242203 26.197660392575795 22.925605860691835 12-13 28.170264543901414 28.313873079766406 27.0408506152883 16.475011761043888 14-15 22.2818070230671 29.665684460747727 32.79058497260318 15.261923543581998 16-17 15.246276759410224 32.92578982921369 32.40377576029793 19.424157651078158 18-19 14.385557850655045 33.39357969497185 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20.612044971477275 44-45 16.91647686278948 28.921159477697454 33.7745978751397 20.387765784373364 46-47 20.170617320628992 31.234924205448156 34.22921574036741 14.365242733555448 48-49 17.947620344787328 30.01942214690785 34.399487736453146 17.633469771851683 50-51 13.223318829813577 31.86352541653401 37.535462874526864 17.377692879125544 52-53 16.898338193245195 33.822155536052996 30.756164538685844 18.523341732015965 54-55 15.411823796150228 29.592385345114213 33.691169292369075 21.304621566366485 56-57 18.96506611979351 29.513825047733537 31.36482568418075 20.1562831482922 58-59 21.67251846587393 29.135241974549093 31.785346811918576 17.406892747658397 60-61 18.11020838194636 30.361398075277712 35.87287782043939 15.655515722336535 62-63 15.588594056605443 31.044248413385894 35.18199657043859 18.18516095957007 64-65 15.139194320504027 34.01510383889239 32.99969594331818 17.846005897285412 66-67 17.354226477534684 30.613146841279043 29.902487660693826 22.13013902049244 68-69 15.63295525258811 29.920942467613283 33.42549640776149 21.02060587203711 70-71 20.21398088597704 30.05773806787847 30.886860965459697 18.841420080684795 72-73 17.712800749553626 27.43330917319285 36.97031838350982 17.8835716937437 74-75 15.354856960995791 34.06343930124828 32.22603345238516 18.35567028537077 76-77 16.128815525191662 29.84313559082294 32.1596791988458 21.86836968513961 78-79 16.833290307066093 29.86317647017219 33.65632503810477 19.64720818465695 80-81 19.013673636083972 28.601130806521763 31.89807890186594 20.48711665552833 82-83 16.33300927971719 28.212107821475918 35.99151568714096 19.46336721166593 84-85 16.745557319155406 29.866975866176944 33.19346297559751 20.19400383907014 86-87 15.883571620494111 31.390621243618572 32.68034166277771 20.045465473109612 88-89 15.699279807382945 30.383501684692106 30.21591636290354 23.70130214502141 90-91 16.467659756524444 27.259998373529548 32.4777863029973 23.794555566948706 92-93 18.95628193116128 29.238248448742198 33.32649778139198 18.478971838704545 94-95 17.081086814332938 27.95170499743676 33.612049002103625 21.355159186126677 96-97 13.895557904934993 28.89182757755015 35.77543075557693 21.43718376193793 98-99 16.985642548978003 29.74644600042436 30.916967253695454 22.350944196902187 100-101 15.790013292983001 26.165610770144525 33.81650026868795 24.227875668184517 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2544.0 1 1585.0 2 605.5 3 552.5 4 428.5 5 446.5 6 795.0 7 1402.0 8 1859.0 9 1828.0 10 1352.5 11 922.0 12 833.5 13 763.5 14 687.0 15 645.0 16 628.5 17 652.5 18 694.5 19 755.5 20 789.5 21 838.0 22 905.0 23 958.5 24 1042.5 25 1237.5 26 1609.0 27 2181.5 28 2364.0 29 2405.5 30 2639.0 31 2768.5 32 3034.0 33 3348.5 34 3710.0 35 4049.5 36 4478.0 37 4708.0 38 4196.5 39 3442.0 40 2958.5 41 2596.5 42 2246.0 43 2241.5 44 2293.5 45 2171.0 46 2891.0 47 7379.0 48 15486.5 49 15026.5 50 6939.5 51 2589.0 52 1245.5 53 908.0 54 789.0 55 678.0 56 573.5 57 512.5 58 484.5 59 442.0 60 417.0 61 393.0 62 314.5 63 201.0 64 115.0 65 85.0 66 62.5 67 44.5 68 37.5 69 35.5 70 29.5 71 22.5 72 13.0 73 10.5 74 9.5 75 6.0 76 3.5 77 2.5 78 2.5 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3599839066018225 2 0.3253972175361572 3 0.3084567984019538 4 0.31198605238824617 5 0.31975041115808944 6 0.23293076309529692 7 0.22375470273093673 8 0.22375470273093673 9 0.23434246468981385 10-11 0.22904858371037531 12-13 0.22340177733230748 14-15 0.22904858371037531 16-17 0.20999061218439646 18-19 0.21810789635286895 20-21 0.2025791788131825 22-23 0.21069646298165495 24-25 0.21210816457617188 26-27 0.2131669407720596 28-29 0.2346953900884431 30-31 0.23787171867610626 32-33 0.24069512186514014 34-35 0.2308132107035215 36-37 0.20328502961044093 38-39 0.1877563120707545 40-41 0.187050461273496 42-43 0.20787305979262102 44-45 0.2110493883802842 46-47 0.21457864236657653 48-49 0.23963634566925243 50-51 0.23151906150078 52-53 0.26892915375547916 54-55 0.22163715033916132 56-57 0.18563875967897905 58-59 0.1859916850776083 60-61 0.1764626993146189 62-63 0.1803448786995405 64-65 0.1778744009091358 66-67 0.17999195330091125 68-69 0.1814036548954282 70-71 0.1824624310913159 72-73 0.1803448786995405 74-75 0.19622652163785617 76-77 0.1955206708405977 78-79 0.20222625341455325 80-81 0.19022678986115915 82-83 0.166227862754371 84-85 0.16411031036259555 86-87 0.17434514692284345 88-89 0.17822732630776505 90-91 0.18528583428034984 92-93 0.1803448786995405 94-95 0.1768156247132481 96-97 0.1866975358748668 98-99 0.19975577562414856 100-101 0.1729334453283265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 141673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.0500518800336 #Duplication Level Percentage of deduplicated Percentage of total 1 79.46522008414668 26.263296464393353 2 9.76443200990966 6.4542996901315 3 3.472652328983619 3.4431401890268436 4 1.785447322896867 2.360365066032342 5 1.1191081306195674 1.849329088817206 6 0.7090532430643061 1.4060547881388832 7 0.5574183627704333 1.2895894065912348 8 0.4100548875552613 1.0841868245890185 9 0.31608397582384723 0.9401932619482893 >10 2.0716314631698096 13.208585969097852 >50 0.1665847980693249 3.82924057512723 >100 0.10678512696751596 6.606057611542073 >500 0.014949917775452235 3.758655495401382 >1k 0.04057834824765606 27.50700556916279 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4890 3.4516103985939455 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4406 3.1099786127208433 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2869 2.025085937334566 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2743 1.9361487368799983 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2738 1.932619482893706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 1.5994579065877055 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1950 1.3764090546540273 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1801 1.2712372858625145 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1705 1.2034756093257006 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1612 1.1378314851806626 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1545 1.0905394817643446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1490 1.0517176879151284 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1393 0.9832501605810564 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1383 0.9761916526084716 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1381 0.9747799510139546 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1281 0.9041948712881072 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1252 0.8837251981676113 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1235 0.8717257346142173 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1030 0.7270263211762298 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 983 0.6938513337050813 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 935 0.6599704954366746 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 833 0.58797371411631 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 768 0.5420934122945092 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 668 0.47150833256866165 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 606 0.4277455831386362 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.375512624141509 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.33033817311696656 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 440 0.31057435079372925 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.2851637220924241 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 391 0.2759876617280639 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 380 0.26822330295822067 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 0.22375470273093673 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.21669619475835197 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 278 0.19622652163785617 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 259 0.18281535648994515 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 255 0.17999195330091125 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 248 0.17505099772010194 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.1637573849639663 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 228 0.16093398177493243 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 226 0.15952228018041548 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 225 0.158816429383157 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 218 0.15387547380234765 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 207 0.14611111503250443 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 205 0.14469941343798748 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 188 0.13269994988459338 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 168 0.11858293393942389 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 162 0.11434782915587303 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 159 0.11223027676409761 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 156 0.11011272437232218 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 155 0.10940687357506369 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 150 0.10587761958877132 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 144 0.10164251480522046 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143 0.100936664007962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.058507972584755E-4 0.0 18-19 0.0 0.0 0.0 7.058507972584755E-4 0.0 20-21 0.0 0.0 0.0 7.058507972584755E-4 0.0 22-23 0.0 0.0 0.0 0.0028234031890339018 0.0 24-25 0.0 0.0 0.0 0.004235104783550853 0.0 26-27 0.0 0.0 0.0 0.00635265717532628 0.0 28-29 0.0 0.0 0.0 0.014822866742427984 0.0 30-31 0.0 0.0 0.0 0.03635131605881149 0.0 32-33 0.0 0.0 0.0 0.06176194476011661 0.0 34-35 0.0 0.0 0.0 0.09140767824497259 0.0 36-37 0.0 0.0 0.0 0.13517042767499807 0.0 38-39 0.0 0.0 0.0 0.2011674772186655 0.0 40-41 0.0 0.0 0.0 0.3049275444156614 0.0 42-43 0.0 0.0 0.0 0.3928059686743416 0.0 44-45 0.0 0.0 0.0 0.501859916850776 0.0 46-47 0.0 0.0 0.0 0.713968081426948 0.0 48-49 0.0 0.0 0.0 0.840315374136215 0.0 50-51 0.0 0.0 0.0 1.0234836560247895 0.0 52-53 0.0 0.0 0.0 1.1805354584148002 0.0 54-55 0.0 0.0 0.0 1.390878995997826 0.0 56-57 0.0 0.0 0.0 1.8108602203666189 0.0 58-59 0.0 0.0 0.0 2.090377136080975 0.0 60-61 0.0 0.0 0.0 2.307779181636586 0.0 62-63 0.0 0.0 0.0 2.5565915876701983 0.0 64-65 0.0 0.0 0.0 2.83822605577633 0.0 66-67 0.0 0.0 0.0 3.2207971878904234 0.0 68-69 0.0 0.0 0.0 3.669718294946814 0.0 70-71 0.0 0.0 0.0 4.018408588792501 0.0 72-73 0.0 0.0 0.0 4.3568640460779395 0.0 74-75 0.0 0.0 0.0 4.89825160757519 0.0 76-77 0.0 0.0 0.0 5.364466059164414 0.0 78-79 0.0 0.0 0.0 6.03749479435037 0.0 80-81 0.0 0.0 0.0 6.665349078511785 0.0 82-83 0.0 0.0 0.0 7.22720631312953 0.0 84-85 0.0 0.0 0.0 7.918234243645578 0.0 86-87 0.0 0.0 0.0 8.489620464026313 0.0 88-89 0.0 0.0 0.0 9.300995955474932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 110 1.5952537E-9 38.869854 9 ATGGGAG 80 1.0646012E-5 35.655895 5 ATGGGGG 155 1.3224053E-9 30.67174 5 GTAAGCG 55 2.7279712E-6 30.22143 94-95 GGGGGAA 85 6.373272E-4 27.945646 9 TTATTCT 70 0.008331833 27.166397 4 GGGGAGA 70 0.008360635 27.147198 7 TGGGGAT 70 0.008360635 27.147198 6 TCTGTTG 35 0.008388986 27.13761 90-91 ATGGGGT 125 7.2819203E-6 26.62307 5 GTACATG 1065 0.0 26.346691 1 CATGGGG 495 0.0 25.93156 4 GAGTACT 1600 0.0 25.377905 12-13 TGGGATA 95 0.0012201072 25.004 8 TACATGG 1065 0.0 24.551792 2 AGAGCTG 175 1.4647776E-7 24.432478 8 ACATGGG 1090 0.0 23.988676 3 GTACTTT 1695 0.0 23.955545 14-15 GCAGAAA 120 2.2737368E-10 23.745409 10-11 ATGGGAT 210 3.3867764E-8 22.622665 7 >>END_MODULE