##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141673 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85914747340707 31.0 31.0 34.0 30.0 34.0 2 32.04201223945282 31.0 31.0 34.0 30.0 34.0 3 32.06796637326802 33.0 31.0 34.0 30.0 34.0 4 35.77249017102765 37.0 35.0 37.0 35.0 37.0 5 35.69805820445674 37.0 35.0 37.0 33.0 37.0 6 35.81995863714328 37.0 35.0 37.0 35.0 37.0 7 35.77901929090229 37.0 35.0 37.0 33.0 37.0 8 35.80570045103866 37.0 35.0 37.0 33.0 37.0 9 37.30790623478009 39.0 37.0 39.0 34.0 39.0 10-11 37.25743790277611 39.0 37.0 39.0 33.5 39.0 12-13 37.20035574880182 39.0 37.0 39.0 33.5 39.0 14-15 38.01249002985749 40.0 37.0 41.0 33.0 41.0 16-17 38.06332540427604 40.0 37.0 41.0 33.0 41.0 18-19 38.177655587161986 40.0 37.0 41.0 33.5 41.0 20-21 38.13848086791414 40.0 37.0 41.0 33.5 41.0 22-23 37.95925829198224 40.0 36.5 41.0 33.0 41.0 24-25 37.88656977688056 40.0 36.5 41.0 33.0 41.0 26-27 37.56705229648557 39.0 36.0 41.0 33.0 41.0 28-29 37.44823643178305 39.0 36.0 41.0 33.0 41.0 30-31 37.349187918657755 39.0 35.5 41.0 33.0 41.0 32-33 36.96434747623047 39.0 35.0 41.0 33.0 41.0 34-35 36.60880337114341 38.0 35.0 40.0 32.0 41.0 36-37 36.281821518567405 38.0 35.0 40.0 31.0 41.0 38-39 36.01668631284719 38.0 35.0 40.0 31.0 41.0 40-41 35.86413783854369 38.0 35.0 40.0 30.5 41.0 42-43 35.415474367028295 38.0 35.0 40.0 29.5 41.0 44-45 34.85893924742188 38.0 35.0 40.0 26.0 41.0 46-47 34.11257967290874 38.0 34.0 40.0 21.5 41.0 48-49 33.3911683948247 38.0 33.0 40.0 11.5 41.0 50-51 32.93296534978437 38.0 33.0 40.0 2.0 41.0 52-53 32.82504076288354 38.0 33.0 40.0 2.0 41.0 54-55 32.63652919045972 38.0 33.0 40.0 2.0 41.0 56-57 32.23133554029349 37.0 32.5 40.0 2.0 41.0 58-59 31.97465995637842 37.0 32.0 40.0 2.0 41.0 60-61 31.3616638314993 36.0 31.0 39.0 2.0 41.0 62-63 31.068665165557306 36.0 31.0 39.0 2.0 40.0 64-65 30.68186951642162 35.5 30.5 39.0 2.0 40.0 66-67 30.12931539531174 35.0 29.5 38.0 2.0 40.0 68-69 29.56170900595032 35.0 29.0 37.0 2.0 39.0 70-71 28.970107218736104 34.5 28.5 37.0 2.0 39.0 72-73 28.387903834887382 34.0 27.0 36.0 2.0 38.5 74-75 27.906679466094456 34.0 26.0 35.5 2.0 37.5 76-77 26.75250753495726 32.0 26.0 34.5 2.0 36.0 78-79 27.341818836334376 33.5 26.0 35.0 2.0 36.0 80-81 27.027686997522466 33.0 26.0 35.0 2.0 36.0 82-83 26.632491723899403 33.0 25.5 35.0 2.0 36.0 84-85 26.200278105214117 33.0 24.5 35.0 2.0 35.0 86-87 25.953745597255654 33.0 23.5 35.0 2.0 35.0 88-89 25.519432072448527 33.0 20.5 35.0 2.0 35.0 90-91 24.959508869015266 32.0 18.0 35.0 2.0 35.0 92-93 24.457310143781807 32.0 9.5 34.0 2.0 35.0 94-95 23.816708194221906 31.0 2.0 34.0 2.0 35.0 96-97 23.13009536044271 31.0 2.0 34.0 2.0 35.0 98-99 22.314703577957694 31.0 2.0 34.0 2.0 35.0 100-101 19.97744806702759 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.038808164073952156 1101 2 0.20698847205552795 1101 3 0.08173717029756489 1101 4 -0.014553150583509478 1101 5 0.21788133651888586 1101 6 0.21696086755595445 1101 7 0.008474789078803724 1101 8 0.2621762569335502 1101 9 0.2290193700833001 1101 10-11 0.46265902548030624 1101 12-13 0.33995419791074255 1101 14-15 0.3642585873377371 1101 16-17 0.44619765825239455 1101 18-19 0.27873513342247236 1101 20-21 0.21394888181780658 1101 22-23 0.13883782256987587 1101 24-25 0.46433378327223807 1101 26-27 0.5243933495842796 1101 28-29 0.4274082259205372 1101 30-31 0.6298638051136223 1101 32-33 0.6281523758307301 1101 34-35 0.2799490624789698 1101 36-37 0.41583693080448114 1101 38-39 0.25985605629929864 1101 40-41 0.20683446857764665 1101 42-43 0.38496155732423887 1101 44-45 0.21085102262484412 1101 46-47 -0.49407386012376975 1101 48-49 -0.4591131091897225 1101 50-51 -0.20654244818771872 1101 52-53 -0.897561381439445 1101 54-55 -0.4438111715672335 1101 56-57 -0.35167683467666677 1101 58-59 -0.5635721979036319 1101 60-61 -0.4265714658779629 1101 62-63 -0.4204654801205834 1101 64-65 -0.1271427854971492 1101 66-67 -0.30043273303713036 1101 68-69 -0.03126167628276022 1101 70-71 -0.16698994557140523 1101 72-73 -0.25918931402804546 1101 74-75 -0.3002011398301683 1101 76-77 -0.5883839267877207 1101 78-79 -0.08290909566185078 1101 80-81 0.2441647644151601 1101 82-83 0.3851822329108643 1101 84-85 0.44840079298648305 1101 86-87 0.7834739716555177 1101 88-89 0.7643161752925245 1101 90-91 0.9674690176099112 1101 92-93 0.8953344233093539 1101 94-95 0.40246092154046664 1101 96-97 0.07740452105535311 1101 98-99 0.6367633958087211 1101 100-101 0.38371321040636275 1102 1 0.392956106710038 1102 2 0.4000641627280288 1102 3 0.22358391329314742 1102 4 0.1659009152990265 1102 5 0.251838587114797 1102 6 0.15987048421806094 1102 7 0.2321080976638541 1102 8 0.16686931308610298 1102 9 0.6630782188973399 1102 10-11 0.511191712753174 1102 12-13 0.2913628962812638 1102 14-15 0.4150772345183853 1102 16-17 0.40102552742515485 1102 18-19 0.3398503692475714 1102 20-21 0.4975446772146199 1102 22-23 0.19856585195521603 1102 24-25 0.42277620443591957 1102 26-27 0.8186569578840803 1102 28-29 0.17124394941708232 1102 30-31 0.22352954697598904 1102 32-33 0.29829031494995917 1102 34-35 0.1245428649876601 1102 36-37 0.24540591990221827 1102 38-39 -0.25977975316496327 1102 40-41 0.0021531352964174744 1102 42-43 0.21419839840118726 1102 44-45 0.7699733703252107 1102 46-47 0.816484539313528 1102 48-49 0.6923659264087334 1102 50-51 0.10710297403103652 1102 52-53 -0.03421098481562268 1102 54-55 0.47399196234702856 1102 56-57 0.5090749921374496 1102 58-59 0.6884109211616618 1102 60-61 0.8638822092421989 1102 62-63 0.7180793207859892 1102 64-65 0.8899726066328952 1102 66-67 0.576828804222064 1102 68-69 0.3980689477647559 1102 70-71 0.6371013365199545 1102 72-73 1.2810592108439813 1102 74-75 0.696281998773248 1102 76-77 0.7095528478596158 1102 78-79 0.6679603506278617 1102 80-81 0.3449619196650495 1102 82-83 0.31568514409057613 1102 84-85 1.0081288223718126 1102 86-87 0.8755571258894257 1102 88-89 1.0968549584584828 1102 90-91 1.431225807106216 1102 92-93 1.6970811311363256 1102 94-95 1.6956890095401533 1102 96-97 1.8608440115012108 1102 98-99 1.6121062897948875 1102 100-101 2.0461531306908363 1103 1 -0.05910763888839554 1103 2 -0.08920871635136507 1103 3 -0.2541087574150964 1103 4 -0.14574826610003555 1103 5 -0.11898219191739656 1103 6 0.018076459460374394 1103 7 -0.15777101802932947 1103 8 -0.16427382409762004 1103 9 0.2970855518192508 1103 10-11 -0.03543768427735472 1103 12-13 0.068707071690568 1103 14-15 -0.055464022242780686 1103 16-17 -0.18531781304749728 1103 18-19 0.03863789601999201 1103 20-21 0.012482782997466302 1103 22-23 -0.04772231009510364 1103 24-25 -0.10399622087354743 1103 26-27 -0.30442359535221186 1103 28-29 0.3119670771623859 1103 30-31 0.44072198172387544 1103 32-33 0.6693652051376304 1103 34-35 0.6044317821111278 1103 36-37 0.2705084719042432 1103 38-39 0.49416804514338253 1103 40-41 0.4481000623665139 1103 42-43 1.008223156088043 1103 44-45 1.4905781548752515 1103 46-47 1.6109086145304374 1103 48-49 1.046106805633329 1103 50-51 1.194335703547857 1103 52-53 1.0341086144147766 1103 54-55 1.471596057166792 1103 56-57 1.0128503578759975 1103 58-59 1.085223641240077 1103 60-61 1.0390373985550099 1103 62-63 1.6219345500305522 1103 64-65 1.127766195771457 1103 66-67 1.3030421849894793 1103 68-69 0.44780213105150324 1103 70-71 1.0971151311255376 1103 72-73 1.1350178996304159 1103 74-75 1.5983402991326336 1103 76-77 1.4567485497509303 1103 78-79 1.4335609604946349 1103 80-81 1.4090160816427613 1103 82-83 1.336921576369953 1103 84-85 0.8180536780729781 1103 86-87 1.1770781626629407 1103 88-89 0.8702785617546667 1103 90-91 0.7821714636205392 1103 92-93 0.6608339898868287 1103 94-95 0.545019245137496 1103 96-97 1.3282996328029846 1103 98-99 1.0803242445631014 1103 100-101 1.8893665477598454 1104 1 -0.008847426440972583 1104 2 0.0907656188593009 1104 3 0.10993915019166423 1104 4 0.21509154283986476 1104 5 0.11428222670382837 1104 6 -0.0629102091073861 1104 7 -0.08683639195104575 1104 8 -0.0016945127694683038 1104 9 0.11532893957144807 1104 10-11 -0.1495925285063393 1104 12-13 0.031938696414314904 1104 14-15 0.2779643658658628 1104 16-17 -0.017926353719055044 1104 18-19 -0.09811622103995887 1104 20-21 0.1391848575679333 1104 22-23 0.2403649501886349 1104 24-25 0.4708950602271855 1104 26-27 0.6249804112065718 1104 28-29 0.44016626444405915 1104 30-31 0.6443293104470555 1104 32-33 0.49320494278645555 1104 34-35 0.9185224643667738 1104 36-37 0.8139069177586649 1104 38-39 0.6209948791064477 1104 40-41 0.790903227690535 1104 42-43 0.8744097961393749 1104 44-45 1.1262450829425532 1104 46-47 0.39557390290286065 1104 48-49 0.8046617354116137 1104 50-51 0.9948810803212211 1104 52-53 0.8228553172022615 1104 54-55 0.6504832033847947 1104 56-57 0.6676232957814747 1104 58-59 0.40245113108109365 1104 60-61 0.6668979531996229 1104 62-63 0.9869092416966225 1104 64-65 1.3016807890558404 1104 66-67 0.8169795914199334 1104 68-69 0.8300114416667483 1104 70-71 1.0640360693161703 1104 72-73 0.9808152903768352 1104 74-75 0.26088396492004406 1104 76-77 0.37303499731633494 1104 78-79 0.3287630707106466 1104 80-81 0.7525678033223784 1104 82-83 0.7511940508833916 1104 84-85 0.29691587241246964 1104 86-87 0.568198858463898 1104 88-89 0.39567600953395043 1104 90-91 0.7649903129824835 1104 92-93 1.409051712849685 1104 94-95 1.3885709668171131 1104 96-97 2.0265066621036176 1104 98-99 2.6516509715895538 1104 100-101 2.824776827933796 1105 1 0.11104678454933037 1105 2 9.918897967082785E-4 1105 3 -0.10134501084271719 1105 4 -0.041079482725812966 1105 5 -0.2539222987149188 1105 6 0.03185911207980041 1105 7 -0.08485284801922433 1105 8 0.21568720924052087 1105 9 0.010788827905265919 1105 10-11 -0.057284271086423644 1105 12-13 0.07476855242371272 1105 14-15 0.3180025674526945 1105 16-17 0.1905661528927567 1105 18-19 0.36078034081722876 1105 20-21 0.3441143315614781 1105 22-23 0.2797419704648618 1105 24-25 0.4085236232598106 1105 26-27 0.05412381408638822 1105 28-29 0.6095388768448871 1105 30-31 0.927498572862568 1105 32-33 0.842712730033 1105 34-35 0.2664140306318359 1105 36-37 0.556119671211512 1105 38-39 0.7081238829266141 1105 40-41 0.7438858900472738 1105 42-43 1.134107122028297 1105 44-45 0.9552195172652063 1105 46-47 0.5423561538579023 1105 48-49 0.6245632928609197 1105 50-51 1.3080606277793478 1105 52-53 1.9124821170847142 1105 54-55 1.6900438851361912 1105 56-57 1.8461055173506722 1105 58-59 1.8337984716629308 1105 60-61 1.6063631309839224 1105 62-63 1.6522780339387566 1105 64-65 1.6579473185886044 1105 66-67 1.423099191772561 1105 68-69 1.3557975127804696 1105 70-71 1.831961429292658 1105 72-73 1.9206110588164478 1105 74-75 1.5676720930422867 1105 76-77 1.6976382885299728 1105 78-79 1.7842067968346562 1105 80-81 1.5109427665900839 1105 82-83 1.4534787996344924 1105 84-85 2.1533293311253665 1105 86-87 2.4679931576117085 1105 88-89 2.096050678943296 1105 90-91 2.093634156649834 1105 92-93 2.260469579437988 1105 94-95 1.90304349106043 1105 96-97 2.4553562066231613 1105 98-99 1.6183347799939014 1105 100-101 1.502965191142728 1106 1 0.1961909087178455 1106 2 0.21281746259789003 1106 3 0.11099793076851228 1106 4 0.14838836649813913 1106 5 0.12523512922300029 1106 6 0.24479333233109202 1106 7 0.08687664200294876 1106 8 0.16694367635064822 1106 9 0.19670900528885937 1106 10-11 0.09628187821143541 1106 12-13 -0.021018587265871247 1106 14-15 0.3554378963514395 1106 16-17 0.04932446774863308 1106 18-19 -0.042785808480630294 1106 20-21 0.3561801113101808 1106 22-23 0.26902504511379277 1106 24-25 0.1944438006433984 1106 26-27 0.3503728902135066 1106 28-29 0.30312719909174035 1106 30-31 0.20475282267779704 1106 32-33 0.6956876006907962 1106 34-35 0.5277776669954761 1106 36-37 0.4751884073534143 1106 38-39 0.2127802022170755 1106 40-41 0.2584461265587308 1106 42-43 0.354986649005383 1106 44-45 0.8260990442422909 1106 46-47 1.2563990215592966 1106 48-49 1.0727340245682413 1106 50-51 1.1065824311792056 1106 52-53 1.8507304541135383 1106 54-55 2.2246891179521597 1106 56-57 1.8305659755916182 1106 58-59 1.4743934457944903 1106 60-61 1.3004503445833393 1106 62-63 0.9993955505684724 1106 64-65 1.0954011249448499 1106 66-67 1.2348693322012423 1106 68-69 1.4322202770081134 1106 70-71 1.7577929148802447 1106 72-73 1.5160471268134934 1106 74-75 1.5133483679868576 1106 76-77 1.4434439056770536 1106 78-79 1.677832073996516 1106 80-81 1.5233965729463286 1106 82-83 1.8047537442021202 1106 84-85 1.5876789246213008 1106 86-87 1.8956723889494747 1106 88-89 1.758892511907078 1106 90-91 1.514679980744436 1106 92-93 1.9988472586693256 1106 94-95 2.4745512515925796 1106 96-97 2.285086435743633 1106 98-99 2.4578211066753504 1106 100-101 1.981512862983081 1107 1 -0.03195354956360319 1107 2 0.28097852527950096 1107 3 0.4900348755588837 1107 4 0.20741700908842375 1107 5 -0.04911107252046065 1107 6 -0.07372057385404673 1107 7 0.22311574536534096 1107 8 0.17300428241125587 1107 9 0.17391946606096553 1107 10-11 0.10304817127909871 1107 12-13 0.36920557213637295 1107 14-15 0.33387293163578846 1107 16-17 0.24870180274240994 1107 18-19 0.2826396792736219 1107 20-21 0.1877077161254519 1107 22-23 0.22035339712707724 1107 24-25 0.05110631621002426 1107 26-27 0.5722076764318516 1107 28-29 0.7776394448368649 1107 30-31 0.4997922581281884 1107 32-33 0.13836090911753018 1107 34-35 -0.14968185977912185 1107 36-37 -0.2975388653738591 1107 38-39 -0.2941313793520379 1107 40-41 0.040160530875873235 1107 42-43 -0.006387365108352583 1107 44-45 0.6880127013614299 1107 46-47 0.29093617390467585 1107 48-49 0.4015011478559174 1107 50-51 0.5744321613576986 1107 52-53 0.4631837542380737 1107 54-55 0.29349360114019873 1107 56-57 -0.25216544823569365 1107 58-59 -0.20090750895851528 1107 60-61 0.24719173105075143 1107 62-63 0.8033806066083251 1107 64-65 0.19741440846498293 1107 66-67 0.22133674606591214 1107 68-69 0.29089607609486734 1107 70-71 0.04621134576442998 1107 72-73 0.41071296326014917 1107 74-75 0.35083280136170103 1107 76-77 0.24186648766128016 1107 78-79 0.15703506278854817 1107 80-81 0.312396157836325 1107 82-83 0.6912419135670014 1107 84-85 0.656628696310797 1107 86-87 0.7747923557406757 1107 88-89 -0.024772909429575662 1107 90-91 -0.026270621165068064 1107 92-93 -0.2907749912268969 1107 94-95 0.2908650747557182 1107 96-97 0.75125497040532 1107 98-99 1.2602910112000494 1107 100-101 0.887988579047839 1108 1 0.1849845050586083 1108 2 0.052840332809445556 1108 3 0.13649821664616013 1108 4 0.133065324760004 1108 5 -0.17093411034246486 1108 6 -0.054120332620307465 1108 7 0.1561733829978067 1108 8 0.15413635788295466 1108 9 -0.18659574028003334 1108 10-11 0.01383476557564478 1108 12-13 0.2573118872910243 1108 14-15 0.46652994895293176 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2316 86-87 -5.077293222218383 2316 88-89 -4.844035646077259 2316 90-91 -5.52369250818985 2316 92-93 -5.789235819301393 2316 94-95 -5.719524335741433 2316 96-97 -4.807695314341483 2316 98-99 -4.214735924308648 2316 100-101 -2.997343112502108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 11.0 6 26.0 7 50.0 8 88.0 9 155.0 10 261.0 11 484.0 12 780.0 13 1093.0 14 1378.0 15 2015.0 16 2771.0 17 3523.0 18 3045.0 19 1596.0 20 1373.0 21 1357.0 22 1371.0 23 1456.0 24 1618.0 25 1752.0 26 1929.0 27 2289.0 28 2773.0 29 3324.0 30 3957.0 31 5014.0 32 6141.0 33 7786.0 34 11108.0 35 16195.0 36 18848.0 37 22596.0 38 12775.0 39 732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.67311452957663 22.216182253316298 17.049184957182895 14.06151825992418 2 15.031798578416494 28.096390984873615 35.21277872283356 21.65903171387632 3 25.565915876701983 26.876680807210974 33.9662462148751 13.591157101211945 4 11.087504323336134 20.980003246913668 40.68029899839772 27.252193431352477 5 8.531712413871004 44.11569524454987 34.75446176437366 12.598130577205465 6 22.931680701333352 41.36709182413021 16.338328404141933 19.362899070394498 7 20.555081066964064 40.85887925010411 21.908197045308565 16.677842637623257 8 24.27067966373268 29.214458647731046 20.137217394987047 26.37764429354923 9 21.225639324359616 22.65428133801077 26.583752726348703 29.536326611280906 10-11 24.231504944484836 27.86945995355502 25.966133278747538 21.932901823212607 12-13 29.531224632412627 28.37922734299449 26.270055835162744 15.819492189430143 14-15 21.984428931412477 30.78885885101607 32.67877436067564 14.54793785689581 16-17 15.243906742992666 33.01334763857616 32.63712916363739 19.105616454793786 18-19 14.763575275458274 33.85860396829318 35.33771431394832 16.040106442300225 20-21 18.377531357421667 27.486888821440925 34.71091880598279 19.42466101515462 22-23 16.683313157551094 28.571025223845304 36.544400249873824 18.201261368729774 24-25 20.903418435411123 29.133991656843577 34.862323801994734 15.100266105750567 26-27 18.176075102789884 26.58702288729288 41.07995553124283 14.156946478674406 28-29 14.699145558563295 34.43282523302146 36.27264481564747 14.595384392767777 30-31 16.73254607441079 28.77083142165409 35.83675082761006 18.659871676325057 32-33 16.893832981584353 30.35229013291171 37.322919681237785 15.430957204266162 34-35 19.46560036139561 28.728127448419954 36.09473929400803 15.711532896176406 36-37 17.421103527136435 27.725113465515662 39.3836510838339 15.470131923514007 38-39 15.78464416171099 31.097072473486385 37.89514549400907 15.223137870793554 40-41 16.045809878416772 31.778926750074998 35.92193262630362 16.253330745204607 42-43 15.212027293682423 31.756263038804892 33.14212694456897 19.889582722943707 44-45 18.179187283392036 28.104861194440716 34.15859055712803 19.557360965039212 46-47 21.14058430328997 31.341187099870833 33.895308209750624 13.622920387088577 48-49 19.199141685430536 30.018422705808444 34.477282192090236 16.305153416670784 50-51 13.7644434719389 32.05550810669641 37.47785393123601 16.702194490128676 52-53 17.87044062891528 34.53175457481162 30.203109283735373 17.39469551253772 54-55 16.01998969457836 30.19241492733266 32.57007333789784 21.217522040191145 56-57 19.78146859316878 30.053362320272743 31.18942917846026 18.975739908098227 58-59 23.06155724802891 29.4735058903249 30.65121794555067 16.813718916095517 60-61 18.263536453664425 31.62705667276051 35.06313835381477 15.046268519760291 62-63 15.875290281140373 31.338716622080426 34.81185546999075 17.97413762678845 64-65 15.722473583533914 35.147487524087154 31.894927050320103 17.235111842058824 66-67 18.208127166079635 30.72533227926281 29.57691303212327 21.489627522534285 68-69 16.531378597192127 30.699921650561507 31.99233446034177 20.776365291904597 70-71 21.047411998051853 30.669570066279388 30.42287521263755 17.860142723031206 72-73 18.89950802199431 27.931927749112397 35.99309677920281 17.175467449690483 74-75 15.75106054082288 35.43688635096313 31.020024987118223 17.79202812109576 76-77 17.258404918368356 30.481813754208638 30.96708617732384 21.29269515009917 78-79 17.541098162670373 31.15166616080693 32.29337982537251 19.013855851150183 80-81 19.638672426449826 29.417245468405895 31.453639392399346 19.490442712744933 82-83 17.21677137008541 29.200959977412293 34.58636267381944 18.995905978682853 84-85 16.343031791744313 31.12894573380767 33.096165791405504 19.43185668304252 86-87 16.313623626237884 32.03115625419099 31.311541366385974 20.343678753185152 88-89 15.534364346064528 31.820812716607964 29.43115484248939 23.213668094838113 90-91 17.61269576819493 27.437373591192593 31.54210803121702 23.407822609395463 92-93 19.21563368003473 30.446782453403543 31.935822007009325 18.40176185955241 94-95 17.74120686369315 29.171754674496903 32.54395685839927 20.54308160341067 96-97 13.706563706563706 30.15041680489578 35.09207823650237 21.050941252038143 98-99 16.66231382790993 31.468906613962023 29.48789440248465 22.380885155643394 100-101 15.200316298181637 28.00862759772237 32.180515890806525 24.610540213289468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3098.0 1 1855.5 2 607.0 3 565.0 4 433.0 5 420.0 6 764.5 7 1352.0 8 1674.0 9 1599.5 10 1269.5 11 891.0 12 774.0 13 741.5 14 680.5 15 628.5 16 612.5 17 651.5 18 675.0 19 731.5 20 824.0 21 901.5 22 948.5 23 961.0 24 1065.0 25 1280.0 26 1663.0 27 2200.0 28 2343.5 29 2338.5 30 2517.5 31 2670.5 32 2958.0 33 3228.0 34 3667.5 35 4144.0 36 4558.5 37 4762.0 38 4163.5 39 3402.0 40 3002.0 41 2679.0 42 2307.5 43 2182.5 44 2198.0 45 2180.0 46 2703.5 47 7627.5 48 16474.0 49 15210.5 50 6596.5 51 2641.0 52 1326.0 53 875.0 54 672.5 55 564.5 56 486.5 57 457.0 58 469.0 59 440.0 60 388.0 61 344.0 62 253.5 63 153.5 64 89.0 65 77.0 66 60.0 67 31.5 68 22.0 69 19.0 70 19.0 71 18.5 72 13.0 73 5.0 74 5.0 75 4.5 76 1.5 77 1.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016940419134203414 2 0.0 3 0.0 4 0.0 5 0.01764626993146189 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0042351047835508535 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010587761958877134 24-25 0.0 26-27 3.529253986292378E-4 28-29 0.0010587761958877134 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.529253986292378E-4 40-41 3.529253986292378E-4 42-43 0.020116747721866553 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.529253986292378E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.058507972584755E-4 82-83 0.0021175523917754267 84-85 7.058507972584755E-4 86-87 0.0 88-89 0.0 90-91 0.013764090546540273 92-93 0.0031763285876631395 94-95 0.0 96-97 0.0 98-99 0.0021175523917754267 100-101 0.024351852505417403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 141673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.855046480275 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68380234877021 25.064761810648466 2 10.044316419233326 6.39924332794534 3 3.6184356304010636 3.4579630557692713 4 1.9122534899180148 2.4365969521362576 5 1.1522269000664747 1.8352120728720362 6 0.7179259915798804 1.3721739498704764 7 0.5628185242632395 1.2550027175255694 8 0.4187901617549302 1.067246405454815 9 0.34123642809660976 0.9783092050002471 >10 2.218036782627964 13.358932188913908 >50 0.16175493020163972 3.575134288114178 >100 0.10857522712164856 6.492415633183457 >500 0.019942388654996677 4.6190876172594635 >1k 0.03766895634832706 23.402483183104756 >5k 0.0022158209616662972 4.685437592201761 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6638 4.685437592201761 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3043 2.147903976057541 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2814 1.98626414348535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2573 1.8161541013460576 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2544 1.7956844282255617 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2525 1.7822732630776505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2333 1.6467499100040233 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2194 1.5486366491850951 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1987 1.4025255341525908 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1871 1.3206468416706076 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1762 1.243709104769434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1704 1.2027697585284423 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1620 1.1434782915587303 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1393 0.9832501605810564 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1323 0.9338406047729632 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1234 0.8710198838169588 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1208 0.8526677630882384 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1027 0.7249087687844543 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 998 0.7044390956639586 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 885 0.6246779555737508 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 844 0.5957380728861533 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 830 0.5858561617245347 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.47292003416317857 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 664 0.4686849293796277 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.41009931320717424 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 550 0.3882179384921615 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 522 0.3684541161689242 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 422 0.29786903644307666 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 408 0.287987125281458 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.26398819817466984 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 351 0.2477536298377249 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.24281267425691555 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 341 0.24069512186514014 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 321 0.22657810591997066 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 321 0.22657810591997066 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.22375470273093673 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 255 0.17999195330091125 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 255 0.17999195330091125 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 239 0.16869834054477564 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 231 0.16305153416670784 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 221 0.15599302619412309 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 213 0.15034621981605528 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 213 0.15034621981605528 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 195 0.13764090546540272 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.13481750227636882 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 181 0.12775899430378407 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 170 0.11999463553394084 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.11928878473668235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 159 0.11223027676409761 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 154 0.10870102277780523 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 147 0.10376006719699589 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 145 0.10234836560247895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.058507972584755E-4 0.0 18-19 0.0 0.0 0.0 7.058507972584755E-4 0.0 20-21 0.0 0.0 0.0 7.058507972584755E-4 0.0 22-23 0.0 0.0 0.0 0.0021175523917754267 0.0 24-25 0.0 0.0 0.0 0.0035292539862923776 0.0 26-27 0.0 0.0 0.0 0.005293880979438566 0.0 28-29 0.0 0.0 0.0 0.013411165147911033 0.0 30-31 0.0 0.0 0.0 0.035998390660182254 0.0 32-33 0.0 0.0 0.0 0.06211487015874584 0.0 34-35 0.0 0.0 0.0 0.0924664544408603 0.0 36-37 0.0 0.0 0.0 0.13587627847225653 0.0 38-39 0.0 0.0 0.0 0.20046162642140705 0.0 40-41 0.0 0.0 0.0 0.3049275444156614 0.0 42-43 0.0 0.0 0.0 0.3924530432757124 0.0 44-45 0.0 0.0 0.0 0.5022128422494053 0.0 46-47 0.0 3.529253986292378E-4 0.0 0.7196148878050158 0.0 48-49 0.0 7.058507972584755E-4 0.0 0.8477268075074291 0.0 50-51 0.0 7.058507972584755E-4 0.0 1.0337184925850373 0.0 52-53 0.0 7.058507972584755E-4 0.0 1.196417101353116 0.0 54-55 0.0 7.058507972584755E-4 0.0 1.4092311167265463 0.0 56-57 0.0 7.058507972584755E-4 0.0 1.8345062220747779 0.0 58-59 0.0 7.058507972584755E-4 0.0 2.1157877647822803 0.0 60-61 0.0 7.058507972584755E-4 0.0 2.3388366167159584 0.0 62-63 0.0 7.058507972584755E-4 0.0 2.5943546053235265 0.0 64-65 0.0 7.058507972584755E-4 0.0 2.880577103611839 0.0 66-67 0.0 7.058507972584755E-4 0.0 3.2663245643135954 0.0 68-69 0.0 7.058507972584755E-4 0.0 3.7166573729645025 0.0 70-71 0.0 7.058507972584755E-4 0.0 4.067465219201965 0.0 72-73 0.0 7.058507972584755E-4 0.0 4.40803822887918 0.0 74-75 0.0 7.058507972584755E-4 0.0 4.958248925342161 0.0 76-77 0.0 7.058507972584755E-4 0.0 5.433639437295744 0.0 78-79 0.0 7.058507972584755E-4 0.0 6.121138113825499 0.0 80-81 0.0 7.058507972584755E-4 0.0 6.758521383749903 0.0 82-83 0.0 7.058507972584755E-4 0.0 7.328848827934751 0.0 84-85 0.0 7.058507972584755E-4 0.0 8.035405475990485 0.0 86-87 0.0 7.058507972584755E-4 0.0 8.610673875756142 0.0 88-89 0.0 7.058507972584755E-4 0.0 9.428402024380087 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 30 1.295208E-4 63.35405 2 GGCATTC 45 2.0351217E-7 63.331684 7 TTAGGCA 55 1.1703378E-8 60.47432 4 GTGTAGG 25 0.0049987333 57.03878 1 CATTCCG 50 4.2160718E-7 56.998516 9 GCATTCC 55 8.140887E-7 51.816833 8 ACCTGGG 40 5.363437E-4 47.515537 3 CTTTAGG 60 1.4796206E-6 47.515537 2 ATGGGTA 85 7.1249815E-9 44.720505 5 GCTGAGG 45 9.581034E-4 42.23603 2 CCTTTAG 70 4.2634365E-6 40.741993 1 CATGGGT 105 1.0095391E-9 40.727604 4 GTACCTG 60 8.220922E-5 39.610268 1 GGCTGAG 50 0.0016056584 38.025856 1 AGGCATT 80 1.0711492E-5 35.624077 6 TAGGCAT 95 8.6064574E-7 35.011448 5 CTCGTAC 55 0.0025723565 34.544556 8 TTTAGGC 105 1.8813389E-6 31.677025 3 TATTAAC 60 0.0039330623 31.677025 2 GGGTGAG 60 0.0039398703 31.665846 8 >>END_MODULE