##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937729_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 131764 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76177104520203 33.0 31.0 34.0 30.0 34.0 2 31.834309826659787 33.0 31.0 34.0 30.0 34.0 3 31.784933669287515 33.0 31.0 34.0 30.0 34.0 4 35.393719073495035 37.0 35.0 37.0 33.0 37.0 5 35.418596885340456 37.0 35.0 37.0 33.0 37.0 6 35.584317415986156 37.0 35.0 37.0 33.0 37.0 7 35.5563735162867 37.0 35.0 37.0 33.0 37.0 8 35.58670046446677 37.0 35.0 37.0 33.0 37.0 9 37.132623478340065 39.0 37.0 39.0 34.0 39.0 10-11 37.04677681309008 39.0 37.0 39.0 33.5 39.0 12-13 36.97754697793024 39.0 37.0 39.0 33.0 39.0 14-15 37.92283931878207 40.0 38.0 41.0 33.0 41.0 16-17 37.92620518502778 40.0 38.0 41.0 33.5 41.0 18-19 37.98311754348684 40.0 38.0 41.0 33.5 41.0 20-21 37.97540299323032 40.0 38.0 41.0 34.0 41.0 22-23 37.78852342066118 40.0 37.5 41.0 33.5 41.0 24-25 37.547437084484386 40.0 36.5 41.0 33.0 41.0 26-27 37.401482954372966 39.0 36.0 41.0 33.0 41.0 28-29 37.41679442032725 39.0 36.0 41.0 33.0 41.0 30-31 37.15751267417504 39.0 35.5 41.0 33.0 41.0 32-33 36.680151634740895 38.5 35.0 41.0 32.5 41.0 34-35 36.437141404328955 38.5 35.0 41.0 32.0 41.0 36-37 36.17858823350839 38.5 35.0 40.5 31.0 41.0 38-39 35.92116207765399 38.0 35.0 40.0 31.0 41.0 40-41 35.714724811025775 38.0 35.0 40.0 30.5 41.0 42-43 35.25100179108102 38.0 35.0 40.0 28.5 41.0 44-45 34.739458425670136 38.0 35.0 40.0 24.5 41.0 46-47 33.9970022160833 38.0 34.5 40.0 18.0 41.0 48-49 33.277173583072766 38.0 33.0 40.0 4.5 41.0 50-51 32.17850095625512 37.0 32.0 39.5 2.0 40.5 52-53 32.35985549922589 37.5 33.0 39.5 2.0 40.5 54-55 32.72954297076592 38.0 33.0 40.0 2.0 41.0 56-57 32.426448043471666 38.0 33.0 40.0 2.0 41.0 58-59 32.177089341550044 38.0 33.0 40.0 2.0 41.0 60-61 31.715119455997083 37.0 32.0 40.0 2.0 41.0 62-63 31.39338893779788 36.0 31.5 40.0 2.0 41.0 64-65 31.04197276949698 36.0 31.0 39.0 2.0 40.5 66-67 30.491093925503172 35.5 30.5 39.0 2.0 40.0 68-69 29.93831395525333 35.0 29.5 38.0 2.0 40.0 70-71 29.29308840047357 35.0 29.0 37.5 2.0 39.0 72-73 28.792807595397832 35.0 28.5 37.0 2.0 39.0 74-75 28.380600922862087 34.5 27.5 36.5 2.0 38.5 76-77 27.910199295710513 34.0 27.0 36.0 2.0 37.5 78-79 27.371205336814306 34.0 26.0 35.0 2.0 37.0 80-81 26.733720894933366 34.0 24.5 35.0 2.0 36.5 82-83 26.15761892474424 33.0 22.0 35.0 2.0 36.0 84-85 25.641366382319905 33.0 20.0 35.0 2.0 36.0 86-87 25.34069624480131 33.0 18.5 35.0 2.0 35.0 88-89 24.72736103943414 32.5 9.0 35.0 2.0 35.0 90-91 24.16410020946541 32.0 2.0 35.0 2.0 35.0 92-93 23.58833596429981 31.5 2.0 34.5 2.0 35.0 94-95 22.86668589296014 31.0 2.0 34.0 2.0 35.0 96-97 22.189262621049757 31.0 2.0 34.0 2.0 35.0 98-99 21.32787028323366 30.5 2.0 34.0 2.0 35.0 100-101 19.370594396041405 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.13416370432050329 1101 2 0.0026626544656203066 1101 3 0.12883667753332517 1101 4 0.0017324648095851103 1101 5 -0.06828940828166452 1101 6 0.141713215669931 1101 7 0.07857448572752901 1101 8 0.058574502716552956 1101 9 0.06957373687374258 1101 10-11 0.1952186667333322 1101 12-13 0.2682518951850099 1101 14-15 0.23086577523968543 1101 16-17 0.3719155637633804 1101 18-19 0.3389033680015885 1101 20-21 0.43894437907844974 1101 22-23 0.03383884456493291 1101 24-25 0.15079460737845807 1101 26-27 0.20212822891780746 1101 28-29 0.20172580688627306 1101 30-31 0.14876671492616822 1101 32-33 0.48744148006773713 1101 34-35 0.10764700373886882 1101 36-37 0.24400755514481176 1101 38-39 0.25235617379438224 1101 40-41 0.6087255685325985 1101 42-43 0.7989848756777462 1101 44-45 0.5882516427472524 1101 46-47 0.6612430185970837 1101 48-49 1.2847798634975547 1101 50-51 0.8136986440715397 1101 52-53 0.9403514723407014 1101 54-55 1.4736874757655372 1101 56-57 1.110004374951579 1101 58-59 1.2018017454981873 1101 60-61 0.7812322731148171 1101 62-63 1.105748633954601 1101 64-65 1.4153680561288091 1101 66-67 1.0631828462414568 1101 68-69 1.2414171161464687 1101 70-71 1.1971828916542009 1101 72-73 1.0433877039479889 1101 74-75 0.9732302773344799 1101 76-77 1.3842360271787477 1101 78-79 1.7063984721891536 1101 80-81 1.8178753883900782 1101 82-83 2.5530341682407283 1101 84-85 2.235069426656537 1101 86-87 2.697075704025778 1101 88-89 2.6961731817970467 1101 90-91 2.7377715598914065 1101 92-93 2.6604219680163617 1101 94-95 3.122850586399565 1101 96-97 3.4513211090027944 1101 98-99 3.3648273656824728 1101 100-101 3.3334784147893757 1102 1 -0.19088466998322673 1102 2 -0.08171564156156919 1102 3 0.1744243958035696 1102 4 -0.052254019037441424 1102 5 0.027417455267695345 1102 6 -0.02884032157761851 1102 7 -0.2642514059088654 1102 8 -0.3836302709074175 1102 9 -0.5410751165970993 1102 10-11 -0.3850774385832807 1102 12-13 -0.1348693204874749 1102 14-15 -0.2769860939739601 1102 16-17 0.03936351094881729 1102 18-19 -0.3504399657223871 1102 20-21 -0.16209472401151714 1102 22-23 0.23505764217421188 1102 24-25 0.1856787825415509 1102 26-27 0.11749601672202203 1102 28-29 -0.2867112414359241 1102 30-31 -0.1769100693232204 1102 32-33 -0.44820053719856645 1102 34-35 0.19192763970121263 1102 36-37 0.6309367320657842 1102 38-39 0.3808768191320908 1102 40-41 0.32873416261829647 1102 42-43 0.137220744266358 1102 44-45 0.15887940164407865 1102 46-47 0.548855815971983 1102 48-49 0.6020578821701505 1102 50-51 0.35338769442507 1102 52-53 0.2709698558713072 1102 54-55 0.15008108489089267 1102 56-57 -0.14002492306787673 1102 58-59 0.043279928239698506 1102 60-61 0.2123037993463086 1102 62-63 0.3101120856519337 1102 64-65 0.7357711968764953 1102 66-67 0.48132417988730225 1102 68-69 0.6169981935323037 1102 70-71 0.8206056816069349 1102 72-73 0.8984992317420399 1102 74-75 0.6546240817799678 1102 76-77 0.7249524614144605 1102 78-79 0.8260515836388187 1102 80-81 0.7985972915894202 1102 82-83 0.7518309962418215 1102 84-85 0.7857550003117559 1102 86-87 1.17779760722512 1102 88-89 1.5950364186422377 1102 90-91 1.5625694035571769 1102 92-93 1.3287488557854132 1102 94-95 1.7816458518396239 1102 96-97 1.7479537899668962 1102 98-99 0.9426233733390212 1102 100-101 1.4069969116056598 1103 1 0.2645044421833802 1103 2 0.24550394192400304 1103 3 0.5633538806409852 1103 4 0.40287933865346304 1103 5 0.036113107441607895 1103 6 0.2592122907716643 1103 7 0.151678444292088 1103 8 0.1257961083399337 1103 9 -0.17001190870524852 1103 10-11 0.601970424040033 1103 12-13 0.47287638649096664 1103 14-15 0.4193785388356872 1103 16-17 0.3397106063086994 1103 18-19 0.20264735616289187 1103 20-21 0.1405906979833702 1103 22-23 0.12312852270033403 1103 24-25 0.19132364918386457 1103 26-27 0.2333527942156266 1103 28-29 0.5648963581256368 1103 30-31 0.2111973475565776 1103 32-33 -0.07291738045761065 1103 34-35 0.4098852575309522 1103 36-37 0.8094533561067081 1103 38-39 1.074738711715213 1103 40-41 0.7105938630202004 1103 42-43 0.6916781794143319 1103 44-45 0.5031979986480977 1103 46-47 0.41995556021750247 1103 48-49 0.3874250725245574 1103 50-51 0.5645495504718347 1103 52-53 0.4610136994957159 1103 54-55 0.414549480944963 1103 56-57 0.6738223549737725 1103 58-59 0.829030750307659 1103 60-61 0.9119018994559198 1103 62-63 1.1687712014721718 1103 64-65 0.8548797098469016 1103 66-67 0.644148440026143 1103 68-69 1.031576198647393 1103 70-71 1.2150704240256402 1103 72-73 1.903395103134077 1103 74-75 1.5489609469608219 1103 76-77 1.132497218447707 1103 78-79 0.9149445321225613 1103 80-81 0.6777533018195996 1103 82-83 0.43264941056407125 1103 84-85 0.7681992823724073 1103 86-87 0.7481300880435349 1103 88-89 0.5723108066583116 1103 90-91 1.0310567985151593 1103 92-93 0.9607364334251649 1103 94-95 1.7823400425593867 1103 96-97 1.456722514848149 1103 98-99 1.7332883349758497 1103 100-101 1.6840703496765421 1104 1 0.20829529185658302 1104 2 0.34092877852530634 1104 3 0.22675257345144217 1104 4 0.583925090287444 1104 5 0.3027797741082736 1104 6 0.4775129443664383 1104 7 0.35690720246201835 1104 8 0.4741621214118297 1104 9 0.46789658802678247 1104 10-11 0.3506632344975458 1104 12-13 0.2761443603471747 1104 14-15 0.3144765780513765 1104 16-17 0.6178151814720962 1104 18-19 0.4300983365550479 1104 20-21 0.3951658613820612 1104 22-23 0.45417427433432067 1104 24-25 0.5733497929747173 1104 26-27 0.3549214216666101 1104 28-29 0.18483099864851482 1104 30-31 0.1049881972297797 1104 32-33 0.08165778294108605 1104 34-35 -0.17475526534486363 1104 36-37 0.16304812734853158 1104 38-39 0.45774524766292757 1104 40-41 0.5318356930855614 1104 42-43 0.959978833009103 1104 44-45 0.6332633581252196 1104 46-47 0.46047843603449934 1104 48-49 0.613242065988608 1104 50-51 0.6884057596221602 1104 52-53 0.46199409165257777 1104 54-55 0.420105036500523 1104 56-57 0.5627112438626583 1104 58-59 0.6368738875625581 1104 60-61 0.4334705269069019 1104 62-63 0.23837904460942738 1104 64-65 -0.6385843424406836 1104 66-67 -0.2502960044183027 1104 68-69 0.376020643091838 1104 70-71 0.14415539134590105 1104 72-73 0.8962055606504187 1104 74-75 0.8646472214706264 1104 76-77 1.0308632315196036 1104 78-79 1.063964139965698 1104 80-81 1.604223890054893 1104 82-83 1.3404925478189718 1104 84-85 1.061990132045608 1104 86-87 0.7121823756252361 1104 88-89 0.5481278001223657 1104 90-91 0.745109086096857 1104 92-93 0.6976645380003283 1104 94-95 0.9843008268731133 1104 96-97 2.063912057331809 1104 98-99 2.2564909493549337 1104 100-101 2.4216520490229456 1105 1 0.5481207074814485 1105 2 0.31088096521653696 1105 3 0.16441981036858166 1105 4 0.23971313257371207 1105 5 0.10591176515973189 1105 6 0.18396554777916663 1105 7 0.3871304774543418 1105 8 0.521492910550748 1105 9 0.24791150226988634 1105 10-11 0.36393252431638246 1105 12-13 0.34551752348267684 1105 14-15 0.45785070622613233 1105 16-17 0.007515580647428521 1105 18-19 -0.10457727865748012 1105 20-21 0.07878651322221941 1105 22-23 0.1044352735886136 1105 24-25 0.579776077134909 1105 26-27 0.9378928652776111 1105 28-29 0.7969729471505147 1105 30-31 0.26064464325337866 1105 32-33 0.6565141236876713 1105 34-35 0.9811643732040736 1105 36-37 0.7273767670818287 1105 38-39 0.9908855731443467 1105 40-41 1.0979408823648455 1105 42-43 0.6460998138399106 1105 44-45 0.24694059635832843 1105 46-47 -0.0268663900391104 1105 48-49 0.07062680959522538 1105 50-51 -0.31683615817403776 1105 52-53 -0.010304895056201246 1105 54-55 0.34619180230304636 1105 56-57 0.5816391729761463 1105 58-59 0.28978874478061556 1105 60-61 0.301282081058762 1105 62-63 0.30029508619384515 1105 64-65 0.8856732353107795 1105 66-67 0.35107698325551695 1105 68-69 0.10488847657870082 1105 70-71 0.5739926506344055 1105 72-73 -0.49261753010713605 1105 74-75 -0.4094677936629445 1105 76-77 -0.24178229201419654 1105 78-79 0.4760578365047152 1105 80-81 0.8918275221117433 1105 82-83 0.4286620438052893 1105 84-85 0.11223797167362903 1105 86-87 0.38176609278100315 1105 88-89 0.6586905934723681 1105 90-91 0.8286288474689698 1105 92-93 0.6771793864452995 1105 94-95 0.4125808637200663 1105 96-97 0.5131378326531255 1105 98-99 1.0451912169340005 1105 100-101 0.882589891721544 1106 1 0.1648779249098027 1106 2 0.34513045919757346 1106 3 0.1716638713039167 1106 4 0.35423321353674453 1106 5 0.42976390109240015 1106 6 0.11850267359146471 1106 7 0.24299497090272837 1106 8 0.2626775275742972 1106 9 0.19805531818551003 1106 10-11 0.06074726811098685 1106 12-13 -0.1076091410533877 1106 14-15 -0.06787636312510159 1106 16-17 -0.28741357669782985 1106 18-19 0.24359040004710408 1106 20-21 0.20609023112996283 1106 22-23 0.3827457029057513 1106 24-25 -0.10848960945292419 1106 26-27 0.1749494328710881 1106 28-29 0.21190849631424413 1106 30-31 0.49527764634275684 1106 32-33 0.6682590901306256 1106 34-35 0.13826994473917154 1106 36-37 -0.37009846462158436 1106 38-39 -0.09617632096452411 1106 40-41 0.19640151941609219 1106 42-43 0.1420516621407586 1106 44-45 -0.5278010676450862 1106 46-47 -0.46529187208874845 1106 48-49 -0.051323760341922764 1106 50-51 -0.3722851834217238 1106 52-53 -0.3655035740803818 1106 54-55 -0.08059524361151915 1106 56-57 -0.04513189339223089 1106 58-59 -0.13063311523856314 1106 60-61 -0.45004954778964645 1106 62-63 -0.36759667901335646 1106 64-65 -0.6017029232063216 1106 66-67 -0.7724715412997227 1106 68-69 -0.32589345588108287 1106 70-71 -0.08950473937305148 1106 72-73 -0.30664224513856553 1106 74-75 -0.19837798861340872 1106 76-77 0.5301629514075579 1106 78-79 0.036886642299961636 1106 80-81 -0.09115426120560954 1106 82-83 -0.30890054275058887 1106 84-85 0.11287715352086636 1106 86-87 -0.2635412471572103 1106 88-89 -0.20327276010172213 1106 90-91 0.3800297210174932 1106 92-93 -0.38846991578118306 1106 94-95 -0.0068289583743172955 1106 96-97 0.10802970439063486 1106 98-99 0.0401043947330848 1106 100-101 0.8667967735794377 1107 1 0.1459524059842856 1107 2 -0.026969662902544655 1107 3 0.2537762034162476 1107 4 0.335307694611231 1107 5 0.12970285103135382 1107 6 0.14933295442174455 1107 7 -0.02532004741228633 1107 8 0.2687825336792997 1107 9 0.14311026324045883 1107 10-11 0.12881808618180912 1107 12-13 0.012506854062607431 1107 14-15 0.5443031240543874 1107 16-17 0.5213471070628515 1107 18-19 0.4621496186063254 1107 20-21 0.4302965803363108 1107 22-23 0.11015718031722344 1107 24-25 0.14166301569969875 1107 26-27 0.2802607881824386 1107 28-29 -0.050148890743138 1107 30-31 0.4766573777224892 1107 32-33 0.5881308850024212 1107 34-35 0.6871099935792202 1107 36-37 0.8918062972831748 1107 38-39 0.43160145681324735 1107 40-41 0.33544303345760795 1107 42-43 0.7385107585998512 1107 44-45 0.6936580288140135 1107 46-47 0.8192014124045315 1107 48-49 0.6750193410011818 1107 50-51 0.8484107872742506 1107 52-53 1.340387756810948 1107 54-55 1.099655061638785 1107 56-57 0.8640561407957961 1107 58-59 1.3638723792669296 1107 60-61 1.0978747501346504 1107 62-63 0.9800834186667409 1107 64-65 0.6661542196508208 1107 66-67 0.6562525124243308 1107 68-69 0.8154374354498088 1107 70-71 0.895537995669681 1107 72-73 1.0240964606001377 1107 74-75 0.7004315351961168 1107 76-77 0.6099859062305093 1107 78-79 0.710268815682138 1107 80-81 0.6313732113218613 1107 82-83 0.5882301043800595 1107 84-85 0.4851299007736145 1107 86-87 0.9881376295216633 1107 88-89 0.9841509273219629 1107 90-91 0.967331308319082 1107 92-93 0.6850954077841394 1107 94-95 1.0116386850933239 1107 96-97 0.8623032086641409 1107 98-99 1.4895854692141626 1107 100-101 1.985691767474428 1108 1 0.8200688301007126 1108 2 0.7169665810336987 1108 3 0.568918268558317 1108 4 0.5409556502591855 1108 5 0.5820590533875531 1108 6 0.5405318456206345 1108 7 0.5283875062952674 1108 8 0.7583875232842914 1108 9 0.4663950865252815 1108 10-11 0.7617875941513148 1108 12-13 0.6345616011173547 1108 14-15 0.9062227359740049 1108 16-17 0.7366104473261856 1108 18-19 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-2.701408560764847 2316 86-87 -2.8881444217603836 2316 88-89 -2.7731140299429917 2316 90-91 -3.1993353583475894 2316 92-93 -2.8114857887970572 2316 94-95 -2.133019434564794 2316 96-97 -1.4419702956093658 2316 98-99 -0.80592735129866 2316 100-101 -0.3927270359443753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 862.0 3 141.0 4 105.0 5 110.0 6 115.0 7 136.0 8 194.0 9 204.0 10 279.0 11 457.0 12 689.0 13 1026.0 14 1192.0 15 1755.0 16 2418.0 17 3212.0 18 2583.0 19 1414.0 20 1301.0 21 1297.0 22 1284.0 23 1432.0 24 1578.0 25 1809.0 26 1810.0 27 2055.0 28 2436.0 29 2954.0 30 3386.0 31 4171.0 32 5194.0 33 6787.0 34 9637.0 35 14790.0 36 16430.0 37 20858.0 38 14256.0 39 1406.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.84834917153996 21.287920321637426 16.493817007797272 15.369913499025342 2 14.836750074221813 25.616802295928075 35.994153604896354 23.552294024953753 3 23.717460800730706 27.188308722788857 34.43979296696605 14.654437509514388 4 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29.45943684682903 34.89625325159347 17.70730334514809 50-51 13.292891644163845 31.415966226752367 38.040543110333545 17.250599018750236 52-53 16.920753740937688 33.48890461084358 30.98569048542803 18.6046511627907 54-55 15.279219668016655 29.30351947977868 33.97182134504592 21.44543950715875 56-57 19.060581291463112 29.173303932936683 31.637234588552854 20.12888018704735 58-59 21.744617922896495 28.835692209436264 31.944998840520206 17.47469102714703 60-61 18.191426670468587 30.04695371162437 36.14219180686247 15.619427811044579 62-63 15.678550924112864 30.6457193480071 35.375215296814936 18.300514431065096 64-65 15.170961291352711 33.58931273332776 33.3680552915504 17.871670683769132 66-67 17.40811570178807 30.20367195014847 30.271727352568806 22.116484995494655 68-69 15.580277705960185 29.374325125849772 33.82203093394978 21.223366234240263 70-71 20.10059612518629 29.52682563338301 31.40188266066486 18.970695580765838 72-73 17.67045216921843 27.14764220347425 37.308427844164534 17.873477783142793 74-75 15.316966339554918 33.531171937082 32.82397180324628 18.327889920116803 76-77 16.07021306531899 29.39373543090303 32.559864015423756 21.976187488354228 78-79 16.859786357776567 29.294615592307665 34.009347322627136 19.83625072728863 80-81 18.943397948242165 28.090754922165523 32.374502840368976 20.59134428922333 82-83 16.28332313027343 27.501872673417164 36.38842098458894 19.826383211720465 84-85 16.754432324123325 29.32691028131903 33.68946545961956 20.229191934938083 86-87 15.83810193722553 30.732875800840286 33.36172316115663 20.067299100777554 88-89 15.416205610248571 29.833867588399247 30.768148511500744 23.981778289851434 90-91 16.47375545652273 26.700077570079245 32.75092399653216 24.075242976865866 92-93 18.858526829676325 28.472193178232093 33.84040971670386 18.82887027538772 94-95 17.233001403004483 27.300033839403515 33.96297436950347 21.50399038808853 96-97 13.930658474547272 28.318701832961366 36.21743081245699 21.533208880034373 98-99 16.895876504248967 29.40932681888177 31.197505750841238 22.497290926028022 100-101 15.646183500678632 25.653064871173903 34.35628770972022 24.344463918427245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2286.0 1 1410.5 2 553.0 3 533.5 4 398.5 5 382.5 6 699.5 7 1306.0 8 1766.5 9 1692.5 10 1220.0 11 852.0 12 785.5 13 739.0 14 666.0 15 594.5 16 594.5 17 651.0 18 667.0 19 706.5 20 765.0 21 806.5 22 859.0 23 915.5 24 997.5 25 1154.5 26 1503.5 27 2060.5 28 2186.0 29 2225.5 30 2420.0 31 2505.0 32 2791.0 33 3093.0 34 3403.0 35 3735.0 36 4191.0 37 4530.0 38 4066.0 39 3183.5 40 2683.0 41 2423.0 42 2146.5 43 2049.5 44 2021.0 45 1944.0 46 2643.0 47 6965.0 48 14563.5 49 13880.5 50 6301.5 51 2365.0 52 1122.0 53 824.0 54 699.0 55 607.0 56 547.0 57 504.5 58 491.5 59 459.0 60 413.5 61 388.5 62 296.5 63 182.5 64 122.5 65 94.0 66 63.0 67 44.5 68 39.0 69 32.5 70 23.5 71 16.5 72 10.5 73 8.0 74 6.5 75 5.5 76 5.0 77 5.0 78 3.5 79 3.0 80 2.5 81 2.0 82 1.0 83 0.5 84 0.5 85 0.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3308946297926596 2 0.30433198749279017 3 0.291430132661425 4 0.2777693451929207 5 0.2754925472815033 6 0.21629580158465134 7 0.2125011383989557 8 0.22084939740748613 9 0.23071552169029477 10-11 0.2219877963631948 12-13 0.2178136668589296 14-15 0.21401900367323395 16-17 0.21933153213320786 18-19 0.21743420054036006 20-21 0.2075680762575514 22-23 0.19997874988616013 24-25 0.2007376825232992 26-27 0.21022434048753835 28-29 0.22881819009744694 30-31 0.22160833004462524 32-33 0.22236726268176438 34-35 0.21895206581463827 36-37 0.20339394675328615 38-39 0.20301448043471665 40-41 0.19921981724902096 42-43 0.2094654078503992 44-45 0.2060502109832731 46-47 0.20680914362041225 48-49 0.220090464770347 50-51 0.22692085850459912 52-53 0.23754591542454692 54-55 0.21363953735466437 56-57 0.18707689505479494 58-59 0.18176436659482104 60-61 0.1908715582404906 62-63 0.1969430193376036 64-65 0.184420630824808 66-67 0.19238942351476881 68-69 0.1958046203818949 70-71 0.18821529401050363 72-73 0.19238942351476881 74-75 0.1969430193376036 76-77 0.21136273944324702 78-79 0.21439846999180354 80-81 0.20263501411614707 82-83 0.20301448043471665 84-85 0.19618408670046444 86-87 0.19846088461188183 88-89 0.20680914362041225 90-91 0.20491181202756445 92-93 0.19618408670046444 94-95 0.19770195197474275 96-97 0.1939072887890471 98-99 0.19846088461188183 100-101 0.18935369296621232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 131764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.57290306912358 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73868029025476 26.770589842445585 2 9.731672581775436 6.534410005767888 3 3.476727626195266 3.501715187759934 4 1.7180188529963607 2.3071552169029474 5 1.0375929651649072 1.7417504022342978 6 0.718855256911635 1.4480434716614552 7 0.5041029002870899 1.184693846574178 8 0.4001175486583629 1.0746486141890046 9 0.2870899925401813 0.8674600042500228 >10 2.0751859303298144 13.323062444977385 >50 0.1604991296878179 3.7354664399987856 >100 0.09268259601690892 5.9469961446222035 >500 0.018084408978909058 4.415470082875443 >1k 0.04068992020254538 27.14853829574087 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4529 3.4372059136031083 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4151 3.1503293767645184 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2753 2.089341550044018 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2664 2.0217965453386357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2456 1.8639385568136972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2108 1.599829999089281 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1833 1.3911235238760207 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1807 1.3713912753104034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1559 1.1831759812998999 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1528 1.159649069548587 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1503 1.1406757536201086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1408 1.0685771530918915 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1324 1.004826811572205 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1297 0.9843356303694484 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1267 0.9615676512552745 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1247 0.946388998512492 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1180 0.8955405118241704 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1158 0.8788439938071096 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 964 0.7316110622021189 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 946 0.7179502747336146 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 804 0.6101818402598586 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 724 0.5494672292887283 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 722 0.54794936401445 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 563 0.42727907470932874 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 548 0.41589508515224194 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.4151361525151028 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 462 0.35062687835827694 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 423 0.32102850550985096 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.27701041255578157 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 355 0.2694210861843903 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 343 0.2603138945387207 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.2337512522388513 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 259 0.19656355301903403 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.19428675510761664 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 240 0.1821438329133906 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 235 0.17834916972769496 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 232 0.17607237181627758 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 231 0.17531343917913847 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 229 0.1737955739048602 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 213 0.16165265171063414 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 205 0.15558119061352113 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 182 0.1381257399593212 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 174 0.1320542788622082 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 166 0.12598281776509515 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 164 0.12446495249081692 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 159 0.12067028930512129 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 158 0.11991135666798215 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 156 0.11839349139370389 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 156 0.11839349139370389 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 0.11080416502231262 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.11080416502231262 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 136 0.10321483865092133 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 133 0.10093804073950396 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7946631856956376E-4 0.0 12-13 0.0 0.0 0.0 7.589326371391275E-4 0.0 14-15 0.0 0.0 0.0 7.589326371391275E-4 0.0 16-17 0.0 0.0 0.0 7.589326371391275E-4 0.0 18-19 0.0 0.0 0.0 7.589326371391275E-4 0.0 20-21 0.0 0.0 0.0 7.589326371391275E-4 0.0 22-23 0.0 0.0 0.0 0.0022767979114173826 0.0 24-25 0.0 0.0 0.0 0.004553595822834765 0.0 26-27 0.0 0.0 0.0 0.008348259008530402 0.0 28-29 0.0 0.0 0.0 0.019732248565617318 0.0 30-31 0.0 0.0 0.0 0.047433289821195476 0.0 32-33 0.0 0.0 0.0 0.08348259008530404 0.0 34-35 0.0 0.0 0.0 0.11991135666798215 0.0 36-37 0.0 0.0 0.0 0.16696518017060807 0.0 38-39 0.0 0.0 0.0 0.22274672900033393 0.0 40-41 0.0 0.0 0.0 0.32634103396982483 0.0 42-43 0.0 0.0 0.0 0.43297106948787223 0.0 44-45 0.0 0.0 0.0 0.5616101514829543 0.0 46-47 0.0 0.0 0.0 0.7634862329619623 0.0 48-49 0.0 0.0 0.0 0.889848517045627 0.0 50-51 0.0 0.0 0.0 1.0442913087034396 0.0 52-53 0.0 0.0 0.0 1.2404753954039038 0.0 54-55 0.0 0.0 0.0 1.4753650465984638 0.0 56-57 0.0 0.0 0.0 1.917063841413436 0.0 58-59 0.0 0.0 0.0 2.169408943262196 0.0 60-61 0.0 0.0 0.0 2.394053003855378 0.0 62-63 0.0 0.0 0.0 2.6809295406939677 0.0 64-65 0.0 0.0 0.0 2.961734616435445 0.0 66-67 0.0 0.0 0.0 3.3393036034121613 0.0 68-69 0.0 0.0 0.0 3.8102213047569897 0.0 70-71 0.0 0.0 0.0 4.181718830636592 0.0 72-73 0.0 0.0 0.0 4.503506268783583 0.0 74-75 0.0 0.0 0.0 5.048040435930907 0.0 76-77 0.0 0.0 0.0 5.533757323699948 0.0 78-79 0.0 0.0 0.0 6.274855043866307 0.0 80-81 0.0 0.0 0.0 6.925639780213109 0.0 82-83 0.0 0.0 0.0 7.517227770863059 0.0 84-85 0.0 0.0 0.0 8.245044169879481 0.0 86-87 0.0 0.0 0.0 8.815761513008106 0.0 88-89 0.0 0.0 0.0 9.593667466075711 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 35 7.898052E-8 47.493725 94-95 TGGGGGA 65 2.5674271E-6 43.857044 6 ATGGGTA 65 1.3169074E-4 36.56145 5 ATGGGGG 105 4.5785782E-8 36.213245 5 GTACCTT 55 0.0025682244 34.554035 6 CTTTGCG 60 0.0039189123 31.698654 2 CGCAGAA 105 1.8811279E-6 31.67453 9 CTGGCTG 60 0.003933556 31.674528 9 TGTAGAA 65 0.0058184355 29.23803 7 ATGGGAG 100 4.8991853E-5 28.517931 5 CATGGGT 105 6.830837E-5 27.159933 4 TTTGCGT 70 0.008339389 27.159931 3 TTCCGGG 35 0.008369859 27.149597 64-65 ATGTAGA 70 0.008354897 27.149597 6 AATAGTA 35 0.008385411 27.13927 72-73 TACATGG 875 0.0 23.909843 2 AGTACTT 1660 0.0 23.60381 12-13 GAGTACT 1650 0.0 23.315102 12-13 GTACATG 895 0.0 22.31302 1 AGAGTAC 2260 0.0 22.174961 10-11 >>END_MODULE