##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937729_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 131764 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933692055493154 31.0 31.0 34.0 30.0 34.0 2 32.09914695971585 33.0 31.0 34.0 30.0 34.0 3 32.10943808627546 33.0 31.0 34.0 30.0 34.0 4 35.76524695668012 37.0 35.0 37.0 35.0 37.0 5 35.7153547251146 37.0 35.0 37.0 33.0 37.0 6 35.86140372180565 37.0 35.0 37.0 35.0 37.0 7 35.82681157220485 37.0 35.0 37.0 35.0 37.0 8 35.84288576545946 37.0 35.0 37.0 35.0 37.0 9 37.38393643180231 39.0 37.0 39.0 34.0 39.0 10-11 37.314376460945326 39.0 37.0 39.0 34.0 39.0 12-13 37.24845557208342 39.0 37.0 39.0 34.0 39.0 14-15 38.08329285692602 40.0 37.0 41.0 33.0 41.0 16-17 38.14921374578792 40.0 37.0 41.0 33.5 41.0 18-19 38.225137366807324 40.0 37.0 41.0 34.0 41.0 20-21 38.18572599496069 40.0 37.0 41.0 34.0 41.0 22-23 38.102751889742265 40.0 37.0 41.0 33.5 41.0 24-25 37.96227345860781 40.0 36.5 41.0 33.0 41.0 26-27 37.6195091223703 39.0 36.0 41.0 33.0 41.0 28-29 37.53180307215932 39.0 36.0 41.0 33.0 41.0 30-31 37.4279507300932 39.0 35.5 41.0 33.0 41.0 32-33 37.02570884308309 39.0 35.0 41.0 33.0 41.0 34-35 36.65547494004432 38.0 35.0 40.0 32.0 41.0 36-37 36.31806107889864 38.0 35.0 40.0 31.5 41.0 38-39 36.05263956771197 38.0 35.0 40.0 31.0 41.0 40-41 35.91152363316232 38.0 35.0 40.0 30.5 41.0 42-43 35.46306654321363 38.0 35.0 40.0 29.5 41.0 44-45 34.87136850733128 38.0 35.0 40.0 26.0 41.0 46-47 34.137518593849606 38.0 34.5 40.0 21.5 41.0 48-49 33.425905406636105 38.0 33.0 40.0 11.0 41.0 50-51 32.970568592331745 38.0 33.0 40.0 2.0 41.0 52-53 32.85701709116299 38.0 33.0 40.0 2.0 41.0 54-55 32.660263804984666 38.0 33.0 40.0 2.0 41.0 56-57 32.22556995841049 37.5 32.5 40.0 2.0 41.0 58-59 31.95534819829392 37.0 32.0 40.0 2.0 41.0 60-61 31.33911766491606 36.0 31.0 39.0 2.0 41.0 62-63 31.01057952096172 36.0 31.0 39.0 2.0 40.0 64-65 30.625561610151482 35.5 30.5 39.0 2.0 40.0 66-67 30.065135393582466 35.0 29.5 38.5 2.0 40.0 68-69 29.481914635256977 35.0 29.0 37.0 2.0 39.0 70-71 28.86676558088704 34.5 28.5 37.0 2.0 39.0 72-73 28.312164172308066 34.0 27.0 36.0 2.0 38.5 74-75 27.880969005191098 34.0 26.0 35.5 2.0 37.5 76-77 26.707852675996477 32.0 26.0 34.5 2.0 36.0 78-79 27.279165781245254 33.5 26.0 35.0 2.0 36.0 80-81 26.964652712425245 33.0 26.0 35.0 2.0 36.0 82-83 26.554756989769587 33.0 24.5 35.0 2.0 36.0 84-85 26.143696305515924 33.0 24.0 35.0 2.0 35.0 86-87 25.987796363194803 33.0 24.0 35.0 2.0 35.0 88-89 25.521269087155822 33.0 20.5 35.0 2.0 35.0 90-91 24.96956680125072 32.0 18.0 35.0 2.0 35.0 92-93 24.495757566558392 32.0 9.0 34.0 2.0 35.0 94-95 23.84711681491151 31.0 2.0 34.0 2.0 35.0 96-97 23.187665067848577 31.0 2.0 34.0 2.0 35.0 98-99 22.461332382137762 31.0 2.0 34.0 2.0 35.0 100-101 20.2967426611214 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2245934004960688 1101 2 0.20398672201457657 1101 3 -0.10717391927556719 1101 4 -0.3027457292091711 1101 5 -0.16498372495725278 1101 6 -0.062162031692750475 1101 7 0.16931106538540774 1101 8 0.24064371941144458 1101 9 0.19166229274564728 1101 10-11 0.4900330265508117 1101 12-13 0.1824066594600211 1101 14-15 0.2789628536174362 1101 16-17 0.21998810580791428 1101 18-19 0.037690800187306195 1101 20-21 0.5205221126554562 1101 22-23 0.191435108604054 1101 24-25 0.36740727964922115 1101 26-27 0.3887014108264495 1101 28-29 0.21878303934771992 1101 30-31 0.8509178134054736 1101 32-33 0.8739096213769884 1101 34-35 0.453344098630609 1101 36-37 0.8557358018890611 1101 38-39 0.6806776424469234 1101 40-41 1.06891611548091 1101 42-43 1.6687525697311898 1101 44-45 2.1396892680432558 1101 46-47 1.6784499412553018 1101 48-49 2.0735730751205494 1101 50-51 1.8449377430789227 1101 52-53 1.8667876990686523 1101 54-55 1.8566236056190135 1101 56-57 1.8575106082922517 1101 58-59 2.0489479673912037 1101 60-61 2.098852269193923 1101 62-63 2.0308301304256737 1101 64-65 2.352080019696487 1101 66-67 2.3922607925916424 1101 68-69 2.311040580172726 1101 70-71 2.656697026740833 1101 72-73 2.3538243085033876 1101 74-75 2.3819742085750235 1101 76-77 2.411280715048484 1101 78-79 2.545355915598382 1101 80-81 2.592866592706681 1101 82-83 2.646509408247585 1101 84-85 2.962026710573891 1101 86-87 2.5157201296720473 1101 88-89 2.6790746487673474 1101 90-91 2.7760267985474947 1101 92-93 2.6048104790896183 1101 94-95 2.8392399122741523 1101 96-97 2.6541319057084856 1101 98-99 3.444327983117862 1101 100-101 4.344468043816832 1102 1 -0.27991849449983164 1102 2 -0.10985366237543559 1102 3 -0.06951619827083988 1102 4 -0.15899011375544347 1102 5 -0.10322349994846292 1102 6 -0.08403788621831865 1102 7 -0.010578774061443141 1102 8 -0.2012876060309523 1102 9 0.2658448780028806 1102 10-11 0.14179676732194224 1102 12-13 -0.22989460230801484 1102 14-15 -0.49575490879515627 1102 16-17 -0.17270301493898899 1102 18-19 0.11814316160766936 1102 20-21 0.17019592803887207 1102 22-23 0.3739813018147231 1102 24-25 0.14905890666589272 1102 26-27 -0.28152008220062186 1102 28-29 0.15051865402016773 1102 30-31 0.21118579262340376 1102 32-33 -0.0438215399965074 1102 34-35 0.4466250861691847 1102 36-37 0.056388171122236486 1102 38-39 -0.09499718860116957 1102 40-41 0.20618710262883866 1102 42-43 0.01253553000307761 1102 44-45 -0.0886008995414187 1102 46-47 0.6106933882649486 1102 48-49 0.6186920250795325 1102 50-51 1.249816839918644 1102 52-53 1.235884148148699 1102 54-55 1.3933242579882474 1102 56-57 1.439349742633432 1102 58-59 1.094691941765209 1102 60-61 1.3751911886829653 1102 62-63 1.3273065552860182 1102 64-65 1.9732566281577562 1102 66-67 1.7871590308040766 1102 68-69 1.655604820963383 1102 70-71 1.7465442863994092 1102 72-73 1.6041075226914394 1102 74-75 1.5562778923126714 1102 76-77 1.3137769063059572 1102 78-79 1.4171282504552103 1102 80-81 1.6765417362669766 1102 82-83 1.6685219868639365 1102 84-85 1.6092785563491177 1102 86-87 1.853702082092063 1102 88-89 1.5454561480446642 1102 90-91 1.5217981177396531 1102 92-93 1.8427494610811017 1102 94-95 2.811758370026407 1102 96-97 2.9796719658670874 1102 98-99 1.6598442142206373 1102 100-101 1.944589142297243 1103 1 0.08197408862037037 1103 2 0.11042401391247125 1103 3 -0.12866855486565498 1103 4 -0.07747750871342163 1103 5 -0.28740325880853135 1103 6 -0.27022714453033814 1103 7 0.07053193109017997 1103 8 0.04164260953353249 1103 9 0.13394864124730788 1103 10-11 0.20995533509687192 1103 12-13 -0.05196255992584753 1103 14-15 0.20535944999183187 1103 16-17 -0.09752946725904366 1103 18-19 -0.19471763488483163 1103 20-21 -0.29838280780329285 1103 22-23 -0.15183163205447414 1103 24-25 -0.1655190984491881 1103 26-27 -0.1435405425586751 1103 28-29 0.12055884400921002 1103 30-31 -0.14456868307991755 1103 32-33 0.1377276013991846 1103 34-35 -0.2845952280434574 1103 36-37 -0.3005720042522668 1103 38-39 -0.28173449221826985 1103 40-41 0.19275999265442323 1103 42-43 0.3756338127944545 1103 44-45 0.6417425421830956 1103 46-47 1.091694484355557 1103 48-49 0.6350603115245477 1103 50-51 0.8847638622058156 1103 52-53 1.0378753794238165 1103 54-55 1.1492614154599394 1103 56-57 1.1426197462870746 1103 58-59 0.9019261397922413 1103 60-61 0.9411941115912548 1103 62-63 1.020711743448242 1103 64-65 1.4773852361226787 1103 66-67 1.3786277922216854 1103 68-69 1.454344316761702 1103 70-71 1.4599896645506725 1103 72-73 1.098681874171163 1103 74-75 1.4045972200437618 1103 76-77 1.3798711700984327 1103 78-79 1.8730288715732222 1103 80-81 2.364009766957512 1103 82-83 2.142051398628645 1103 84-85 2.0716826484207296 1103 86-87 1.7644437700716011 1103 88-89 2.252197105297128 1103 90-91 2.401794464104288 1103 92-93 2.0706632352864354 1103 94-95 1.2828581142719315 1103 96-97 1.6042243955346045 1103 98-99 2.081619881009093 1103 100-101 2.0865694126662575 1104 1 0.2617126507118783 1104 2 0.1424501577033226 1104 3 0.048455628140885665 1104 4 -0.02453633224283891 1104 5 0.37860981308689645 1104 6 -0.15650165433426366 1104 7 -0.11901055256995363 1104 8 0.2305314984224225 1104 9 -0.022914103850730783 1104 10-11 -0.08710348843253968 1104 12-13 0.18659953157742137 1104 14-15 0.2017646787500027 1104 16-17 0.1197907941788614 1104 18-19 0.17913857426548674 1104 20-21 -0.007533134600677727 1104 22-23 -0.35215842944009523 1104 24-25 0.059971097629237136 1104 26-27 0.1058058626700813 1104 28-29 0.16631047799614151 1104 30-31 0.2887646502534196 1104 32-33 0.6043942680658532 1104 34-35 0.28828058895000197 1104 36-37 0.1308005447673466 1104 38-39 0.26009557314121423 1104 40-41 0.9656358096478854 1104 42-43 0.5377253160624278 1104 44-45 1.2198471173465038 1104 46-47 1.5263350072313742 1104 48-49 1.6598969128317407 1104 50-51 1.5635220321404617 1104 52-53 1.6656531572015894 1104 54-55 1.2669084742834613 1104 56-57 1.1383713802740019 1104 58-59 1.832645094040604 1104 60-61 1.8539392096304788 1104 62-63 1.7256137042325541 1104 64-65 2.1231368701096045 1104 66-67 2.2567323673850836 1104 68-69 1.6154554278728135 1104 70-71 2.265218422720608 1104 72-73 2.0669825277659335 1104 74-75 2.253290030501276 1104 76-77 2.173335222386015 1104 78-79 2.260610570919628 1104 80-81 2.2993038846045692 1104 82-83 1.9070840783672054 1104 84-85 1.4742970275056955 1104 86-87 1.8703261230127808 1104 88-89 2.074746124904973 1104 90-91 2.016500346457228 1104 92-93 2.4941926470511397 1104 94-95 2.2103090946640904 1104 96-97 1.7212178595868934 1104 98-99 0.979659096695368 1104 100-101 0.9467001316205099 1105 1 -0.3449899855999199 1105 2 0.08077537593378992 1105 3 -0.39116362000580196 1105 4 -0.4485394905531379 1105 5 -0.18321849765575138 1105 6 -0.2862555693230746 1105 7 -0.06014315773730061 1105 8 -0.08315012635276986 1105 9 0.2539644327988313 1105 10-11 0.15332288345850031 1105 12-13 -0.17395229541735802 1105 14-15 0.27995479148413693 1105 16-17 0.1469434897089812 1105 18-19 -0.0311961188958918 1105 20-21 -0.06328608455023499 1105 22-23 -0.033059425185150815 1105 24-25 0.13096728133762525 1105 26-27 -0.45339052281924097 1105 28-29 -0.26811071777523665 1105 30-31 0.2803424894427806 1105 32-33 -0.1913683322762978 1105 34-35 -0.3257914380711 1105 36-37 -0.07060753524318386 1105 38-39 0.06603495112953794 1105 40-41 0.05547219162796324 1105 42-43 0.0026768447723455324 1105 44-45 -0.3249175446704342 1105 46-47 -0.2910532456089143 1105 48-49 -0.24401588271153685 1105 50-51 -0.3760533505853445 1105 52-53 0.10757533994492974 1105 54-55 -0.22420877798656846 1105 56-57 -0.9000766611349604 1105 58-59 -0.4968500149649664 1105 60-61 0.3206453551759374 1105 62-63 0.17067226456943985 1105 64-65 0.31149498740574444 1105 66-67 0.1715018545983149 1105 68-69 0.08034115845140022 1105 70-71 -0.016638839199817568 1105 72-73 -0.22992057351932615 1105 74-75 -0.046310794168636704 1105 76-77 -0.011601392080805795 1105 78-79 0.06027719372087503 1105 80-81 0.3555612868943463 1105 82-83 0.2759045371995086 1105 84-85 0.6564043223251907 1105 86-87 0.5387035410941081 1105 88-89 -0.11580526343559683 1105 90-91 -0.05515381856448798 1105 92-93 0.5340663146389808 1105 94-95 0.3534858284448461 1105 96-97 0.3651403055227611 1105 98-99 0.08868660031426501 1105 100-101 0.06529029699314748 1106 1 0.008211250151649807 1106 2 -0.5839737451911731 1106 3 -0.5836638863315748 1106 4 -0.2542282089935384 1106 5 -0.28852370698780305 1106 6 -0.25173974957235856 1106 7 0.1246869264216457 1106 8 -0.18403432790810825 1106 9 -0.4556872130944285 1106 10-11 -0.37631917470704224 1106 12-13 -0.6070512620733766 1106 14-15 -0.5580392428186229 1106 16-17 -0.32418679685755336 1106 18-19 -0.4382750578540211 1106 20-21 -0.37121193945595365 1106 22-23 -0.5620090363495223 1106 24-25 -0.5945573804286468 1106 26-27 -0.4807954445194653 1106 28-29 -0.46300791602813973 1106 30-31 -0.5998908119314521 1106 32-33 -0.793131408871588 1106 34-35 -1.325631642609288 1106 36-37 -0.9520659351579539 1106 38-39 -1.177812923590821 1106 40-41 -1.2141027524436225 1106 42-43 -0.8194175410804263 1106 44-45 -0.6781454129989726 1106 46-47 -0.15690495542035165 1106 48-49 0.0826326738027916 1106 50-51 -0.2726124216410497 1106 52-53 -0.5201544991943052 1106 54-55 -0.36754530722914325 1106 56-57 -0.03459780740107021 1106 58-59 -0.3758049526447351 1106 60-61 -0.9891980452714861 1106 62-63 -0.7139287794275795 1106 64-65 -1.2662188721873093 1106 66-67 -1.2985897614664523 1106 68-69 -0.661855496496937 1106 70-71 -0.19393190407677707 1106 72-73 -0.7325039334852335 1106 74-75 -0.5945157584809806 1106 76-77 -0.9417441426933522 1106 78-79 -0.47935208080772895 1106 80-81 -0.35779228719748346 1106 82-83 -1.3989383305963798 1106 84-85 -0.9222949426156895 1106 86-87 -0.25292317670710673 1106 88-89 -0.4065797387738357 1106 90-91 -1.1179534350553766 1106 92-93 -0.5268157563102029 1106 94-95 0.6388805232355175 1106 96-97 -0.3462424578734229 1106 98-99 0.2576703011771606 1106 100-101 0.3962799635532832 1107 1 -0.023229531289128857 1107 2 -0.03452319574061846 1107 3 -0.02444532711301406 1107 4 -0.006059710825041975 1107 5 0.08235541389132095 1107 6 0.34990860207599184 1107 7 0.03433283606755566 1107 8 0.19081304693926882 1107 9 0.0736168162096007 1107 10-11 0.18137313298526436 1107 12-13 0.06587303585092741 1107 14-15 0.2218752870959051 1107 16-17 0.1756605779898237 1107 18-19 0.1391058945269279 1107 20-21 0.014777071533053743 1107 22-23 0.12037802103753137 1107 24-25 0.18779915192788366 1107 26-27 0.5416404529164325 1107 28-29 -0.1450897231099404 1107 30-31 0.2915927045520661 1107 32-33 0.4292434385032564 1107 34-35 0.1624635954859528 1107 36-37 0.3672258841338589 1107 38-39 0.7420588712809817 1107 40-41 0.5262818629409978 1107 42-43 0.3272552305923426 1107 44-45 0.6693820595285018 1107 46-47 -0.006721805237198453 1107 48-49 0.5453921365622563 1107 50-51 -0.09755137158000338 1107 52-53 -0.004281483321278756 1107 54-55 -0.12807644276028185 1107 56-57 0.5096634868602195 1107 58-59 0.375568673728889 1107 60-61 0.981955800882357 1107 62-63 1.025387459888666 1107 64-65 0.693335462367024 1107 66-67 0.8482356353589395 1107 68-69 0.39141068176924065 1107 70-71 1.3701706600257886 1107 72-73 1.6490589480776485 1107 74-75 1.2531643391991167 1107 76-77 1.0718394958902842 1107 78-79 0.9602083587527126 1107 80-81 0.9344848800796868 1107 82-83 1.3127527560947065 1107 84-85 0.735672090351347 1107 86-87 1.3963442225602876 1107 88-89 1.3875289303348382 1107 90-91 1.929207737105795 1107 92-93 1.1947959653015197 1107 94-95 0.8353090946640904 1107 96-97 0.9498503382193739 1107 98-99 1.3542820227888832 1107 100-101 1.5163959586692783 1108 1 -0.010235768295366654 1108 2 0.36551145429402965 1108 3 0.478326675658991 1108 4 0.22904077427544678 1108 5 0.2864441179800181 1108 6 0.3254803276477176 1108 7 0.08682763856235454 1108 8 0.21246931859553797 1108 9 0.021468514061297128 1108 10-11 -0.2152831386710119 1108 12-13 0.461749681727575 1108 14-15 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-2.6964398732796546 2316 60-61 -2.0725313786048183 2316 62-63 -1.6805954460942445 2316 64-65 -1.887647443615883 2316 66-67 -1.3795421424188348 2316 68-69 -1.440426925068362 2316 70-71 -0.9772652374101121 2316 72-73 -1.576154727136025 2316 74-75 -1.8865792505444716 2316 76-77 -1.6044425553917634 2316 78-79 -1.6991933506490007 2316 80-81 -2.465728795133991 2316 82-83 -2.5925891242471764 2316 84-85 -2.6230885934093386 2316 86-87 -2.3822882560721865 2316 88-89 -2.9994368816309773 2316 90-91 -2.6338264509283924 2316 92-93 -2.641101470595917 2316 94-95 -2.3341353497803574 2316 96-97 -2.634337695968661 2316 98-99 -2.0843931908863347 2316 100-101 -2.523561306287988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 6.0 6 23.0 7 53.0 8 92.0 9 151.0 10 295.0 11 473.0 12 711.0 13 1033.0 14 1287.0 15 1833.0 16 2580.0 17 3339.0 18 2817.0 19 1579.0 20 1303.0 21 1233.0 22 1301.0 23 1395.0 24 1502.0 25 1624.0 26 1838.0 27 2156.0 28 2459.0 29 2939.0 30 3602.0 31 4349.0 32 5514.0 33 6918.0 34 9952.0 35 14659.0 36 17662.0 37 21876.0 38 12584.0 39 623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.868926141885325 22.188562925170068 16.99541423712342 13.947096695821184 2 15.28338544670775 28.378009167906253 34.99514283112231 21.343462554263684 3 25.668619653319567 26.94969794481042 33.91138702528764 13.470295376582374 4 11.044746668285722 21.184845633101606 40.68561974439149 27.084787954221184 5 8.57500664111419 44.51519866418732 34.40173048461159 12.508064210086904 6 22.928872833247322 41.32995355332261 16.15539904678061 19.585774566649462 7 20.981451686348322 40.944415773655926 21.522570656628517 16.55156188336723 8 24.24106736286087 29.21966546249355 20.1974742721836 26.34179290246198 9 21.187122431013023 23.054096718375277 26.151300810540057 29.607480040071643 10-11 24.394371755562975 28.00044018092954 25.905786102425548 21.699401961081936 12-13 29.615841463923708 28.444353366652624 26.07215638056855 15.867648788855115 14-15 21.923666555356547 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15.094031753741538 40-41 15.826841272431288 32.075650692338925 35.813787581538136 16.283720453691654 42-43 15.28157867767905 32.09317508094177 32.939220605706346 19.686025635672834 44-45 18.165432136243588 28.344995598190703 33.827145502565195 19.662426763000514 46-47 21.197747487932972 31.51467775720227 33.600983576697736 13.686591178167026 48-49 19.25108527367111 30.299626605142528 34.18422330833915 16.265064812847214 50-51 13.918065632494459 32.45195956406909 37.20287787256003 16.427096930876413 52-53 17.933578215597585 34.842597371057344 29.870070732521782 17.35375368082329 54-55 15.911781670258948 30.729941410400414 32.0292340851826 21.32904283415804 56-57 19.803208767189826 30.258264776418446 30.80962933730002 19.128897119091707 58-59 23.2620442609514 29.67274824686561 30.265474636471268 16.799732855711728 60-61 18.166570535199295 32.05200206429677 34.82020885826174 14.961218542242191 62-63 15.988054400291428 31.538204668953583 34.581524543881486 17.892216386873503 64-65 15.783522054582436 35.64061503900914 31.430815700798398 17.14504720561003 66-67 18.349852767068395 30.96786679214353 29.091785313135603 21.59049512765247 68-69 16.524619774748793 30.990634771257703 31.45510154518685 21.029643908806655 70-71 21.054309219513677 31.038447527397466 30.145942746121857 17.761300506967 72-73 19.032512674175038 28.074436113050606 35.66148568653046 17.23156552624389 74-75 15.689793873895752 35.80416502231262 30.59371300203394 17.912328101757687 76-77 17.262302298048024 30.726146747214717 30.649115084545098 21.36243587019216 78-79 17.432682675085758 31.55148599010352 31.797000698218024 19.218830636592696 80-81 19.683676876840412 29.283036337694668 31.22552442245226 19.80776236301266 82-83 17.304043593090675 29.303527518897422 34.33714823472268 19.055280653289213 84-85 16.31022130475699 31.30407395039616 32.821180292037276 19.564524452809568 86-87 15.975911478097204 32.170016089371906 31.1253453143499 20.72872711818099 88-89 15.488297258735315 31.87251449561337 29.23294678364348 23.40624146200783 90-91 17.67931688804554 27.60645161290323 31.188614800759012 23.52561669829222 92-93 19.30032938176409 30.652995552587242 31.497320927126182 18.549354138522485 94-95 17.852372423423695 29.33198749279014 32.103230017303666 20.712410066482498 96-97 13.566300355180475 30.40284144379345 34.80085607601469 21.230002125011385 98-99 16.93432097266283 31.65252500720997 29.09716003096492 22.315993989162276 100-101 15.13635777121648 28.28142908105564 31.81921684415062 24.762996303577257 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2804.0 1 1708.0 2 596.5 3 535.5 4 406.5 5 415.0 6 725.0 7 1199.5 8 1477.5 9 1407.0 10 1091.5 11 806.0 12 715.0 13 687.0 14 663.5 15 625.0 16 594.0 17 589.5 18 650.5 19 734.5 20 774.0 21 799.0 22 823.0 23 911.0 24 1038.5 25 1177.0 26 1510.0 27 2043.5 28 2197.0 29 2220.5 30 2427.0 31 2564.5 32 2861.5 33 3102.5 34 3389.5 35 3680.0 36 4184.5 37 4492.0 38 3901.0 39 3152.0 40 2688.5 41 2428.5 42 2170.5 43 2105.0 44 2152.0 45 2037.0 46 2440.5 47 7067.5 48 15255.5 49 14009.0 50 6081.5 51 2497.5 52 1256.0 53 822.0 54 632.0 55 528.0 56 486.0 57 472.5 58 444.5 59 416.0 60 402.0 61 364.0 62 248.0 63 145.0 64 99.0 65 72.5 66 45.0 67 29.5 68 29.0 69 21.5 70 14.0 71 10.0 72 5.0 73 3.5 74 1.0 75 1.5 76 2.0 77 1.0 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03946449713123463 2 0.0 3 0.0 4 0.0 5 0.0068303937342521475 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006450927415682583 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0011383989557086913 22-23 0.001517865274278255 24-25 0.0 26-27 0.001517865274278255 28-29 0.0011383989557086913 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.7946631856956376E-4 42-43 0.02542424334416077 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010625056919947785 92-93 0.001517865274278255 94-95 0.0 96-97 0.0 98-99 0.001517865274278255 100-101 0.011383989557086912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 131764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.217449379193106 #Duplication Level Percentage of deduplicated Percentage of total 1 78.65774657840805 25.341519686712605 2 10.178794374690819 6.55869585015634 3 3.5499752656003394 3.4311344525059955 4 1.8586134602247297 2.3951914028110863 5 1.1071588419589644 1.7834916972769497 6 0.819768674471744 1.5846513463464982 7 0.5535794209794822 1.2484441880938648 8 0.40752868012532095 1.0503627698005524 9 0.3203693670349344 0.9289335478582921 >10 2.195472427033521 13.106007710755593 >50 0.18138559751242608 3.9009137548951154 >100 0.11071588419589644 6.938921101363044 >500 0.018845256884407906 4.4359612640782 >1k 0.03769051376881581 22.402173583072766 >5k 0.0023556571105509882 4.893597644273094 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6448 4.893597644273094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2743 2.081752223672627 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2683 2.036216265444279 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2443 1.8540724325308886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2337 1.7736255729941408 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2302 1.7470629306942715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 1.6476427552290458 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2011 1.5262135332867854 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1859 1.410855772441638 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1732 1.3144713275249689 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1618 1.2279530068911084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1540 1.1687562611942566 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1355 1.0283537233235178 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1261 0.9570140554324399 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1240 0.9410764700525182 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1142 0.8667010716128836 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1081 0.8204061807473968 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 920 0.6982180261679973 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 851 0.6458516742053976 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 831 0.6306730214626151 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 817 0.6200479645426672 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 729 0.553261892474424 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 599 0.4546006496463374 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.4363862663549984 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 523 0.39692176922376365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.36808232901247684 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 477 0.3620108679153638 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 388 0.29446586320998147 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 382 0.28991226738714676 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.2633496250872773 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.2603138945387207 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 336 0.25500136607874685 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 308 0.2337512522388513 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 275 0.20870647521326005 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 267 0.20263501411614707 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 264 0.20035821620472968 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 254 0.1927688898333384 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 225 0.17075984335630368 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.16772411280774718 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 219 0.16620624753346894 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 207 0.1570990558877994 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.15330439270210378 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 202 0.15330439270210378 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 197 0.14950972951640812 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 180 0.13660787468504296 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 175 0.13281321149934733 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 147 0.11156309765945176 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 146 0.11080416502231262 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 145 0.1100452323851735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 142 0.10776843447375611 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.10700950183661698 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 140 0.10625056919947785 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1047327039251996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 132 0.10017910810236484 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7946631856956376E-4 0.0 12-13 0.0 0.0 0.0 7.589326371391275E-4 0.0 14-15 0.0 0.0 0.0 7.589326371391275E-4 0.0 16-17 0.0 0.0 0.0 7.589326371391275E-4 0.0 18-19 0.0 0.0 0.0 7.589326371391275E-4 0.0 20-21 0.0 0.0 0.0 7.589326371391275E-4 0.0 22-23 0.0 0.0 0.0 0.001897331592847819 0.0 24-25 0.0 0.0 0.0 0.0037946631856956375 0.0 26-27 0.0 0.0 0.0 0.007589326371391276 0.0 28-29 0.0 0.0 0.0 0.02011171488418688 0.0 30-31 0.0 0.0 0.0 0.0481922224583346 0.0 32-33 0.0 0.0 0.0 0.08310312376673447 0.0 34-35 0.0 0.0 0.0 0.12067028930512128 0.0 36-37 0.0 0.0 0.0 0.16810357912631674 0.0 38-39 0.0 0.0 0.0 0.2280592574603078 0.0 40-41 0.0 0.0 0.0 0.33544822561549437 0.0 42-43 0.0 0.0 0.0 0.4451139916820983 0.0 44-45 0.0 0.0 0.0 0.5775477368628761 0.0 46-47 0.0 0.0 0.0 0.779423818341884 0.0 48-49 0.0 0.0 0.0 0.9073039676998269 0.0 50-51 0.0 0.0 0.0 1.0628851583133483 0.0 52-53 0.0 0.0 0.0 1.2658996387480648 0.0 54-55 0.0 0.0 0.0 1.5053428857654594 0.0 56-57 0.0 0.0 0.0 1.94855954585471 0.0 58-59 0.0 0.0 0.0 2.2016635803406093 0.0 60-61 0.0 0.0 0.0 2.432379102030904 0.0 62-63 0.0 0.0 0.0 2.7203940378252027 0.0 64-65 0.0 0.0 0.0 3.007270574663793 0.0 66-67 0.0 0.0 0.0 3.3901520901004827 0.0 68-69 0.0 0.0 0.0 3.861449257763881 0.0 70-71 0.0 0.0 0.0 4.2393977110591665 0.0 72-73 0.0 0.0 0.0 4.565738745028991 0.0 74-75 0.0 0.0 0.0 5.11482650799915 0.0 76-77 0.0 0.0 0.0 5.608891654776722 0.0 78-79 0.0 0.0 0.0 6.356440302358763 0.0 80-81 0.0 0.0 0.0 7.016332230351234 0.0 82-83 0.0 0.0 0.0 7.615130081054005 0.0 84-85 0.0 0.0 0.0 8.350535806441819 0.0 86-87 0.0 0.0 0.0 8.929601408578975 0.0 88-89 0.0 0.0 0.0 9.71775295224796 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 55 8.138177E-7 51.815285 2 GCTTTGC 60 1.4827365E-6 47.497345 1 CATTCCG 45 9.578746E-4 42.23589 9 GGCATTC 45 9.578746E-4 42.23589 7 ATGGGTA 70 4.2827905E-6 40.71201 5 CGCAGAA 60 8.235083E-5 39.596146 9 ACATGAG 50 0.0016110695 37.997875 3 TTTGCGT 75 6.878412E-6 37.997875 3 TTGCGTT 80 1.0708001E-5 35.62301 4 TTAGGCA 55 0.002572084 34.54352 4 CATGGGT 115 1.0250915E-7 33.04163 4 TACATGA 75 3.0647893E-4 31.664896 2 CATGGGC 105 1.8856499E-6 31.664894 4 AGGCATT 65 0.0058271484 29.229134 6 TGGGATG 85 6.378968E-4 27.939615 6 CGATTCC 35 0.008382328 27.14134 74-75 ACTTATC 35 0.008382328 27.14134 24-25 CACAAAG 35 0.008382328 27.14134 56-57 TATCACT 45 8.9320994E-4 26.387413 82-83 GTACATG 1100 0.0 26.339437 1 >>END_MODULE