##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937728_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 106114 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94018696873174 31.0 31.0 34.0 30.0 34.0 2 32.13609891249034 33.0 31.0 34.0 30.0 34.0 3 32.16622688806378 34.0 31.0 34.0 30.0 34.0 4 35.84647643100816 37.0 35.0 37.0 35.0 37.0 5 35.70094426748591 37.0 35.0 37.0 33.0 37.0 6 35.91082232316188 37.0 35.0 37.0 35.0 37.0 7 35.8836251578491 37.0 35.0 37.0 35.0 37.0 8 35.931554743012235 37.0 35.0 37.0 35.0 37.0 9 37.467299319599675 39.0 37.0 39.0 35.0 39.0 10-11 37.42389317149481 39.0 37.0 39.0 34.5 39.0 12-13 37.38360631019469 39.0 37.0 39.0 34.0 39.0 14-15 38.22292534444088 40.0 37.0 41.0 33.0 41.0 16-17 38.27579772697288 40.0 37.5 41.0 33.5 41.0 18-19 38.359910096688466 40.0 37.0 41.0 34.0 41.0 20-21 38.349393105528016 40.0 37.5 41.0 34.0 41.0 22-23 38.17003411425448 40.0 37.0 41.0 34.0 41.0 24-25 38.00050417475545 40.0 37.0 41.0 33.5 41.0 26-27 37.69934221686111 39.5 36.5 41.0 33.0 41.0 28-29 37.623056335639035 39.0 36.0 41.0 33.0 41.0 30-31 37.537794259004464 39.0 35.5 41.0 33.0 41.0 32-33 37.140881504796724 39.0 35.0 41.0 33.0 41.0 34-35 36.78669167122152 38.5 35.0 41.0 33.0 41.0 36-37 36.44833857926381 38.5 35.0 40.5 32.0 41.0 38-39 36.19778728537233 38.0 35.0 40.0 31.0 41.0 40-41 36.02059106244228 38.0 35.0 40.0 31.0 41.0 42-43 35.585742691822006 38.0 35.0 40.0 30.0 41.0 44-45 34.9849737075221 38.0 35.0 40.0 26.5 41.0 46-47 34.20681531183444 38.0 34.5 40.0 21.5 41.0 48-49 33.444422979060256 38.0 33.0 40.0 8.5 41.0 50-51 32.9729630397497 38.0 33.0 40.0 2.0 41.0 52-53 32.88394085606046 38.0 33.0 40.0 2.0 41.0 54-55 32.681305011591306 38.0 33.0 40.0 2.0 41.0 56-57 32.23683962530863 37.5 32.5 40.0 2.0 41.0 58-59 31.966144900767098 37.0 33.0 40.0 2.0 41.0 60-61 31.333113444031888 36.0 31.0 39.5 2.0 41.0 62-63 31.013193358086586 36.0 31.0 39.0 2.0 40.0 64-65 30.642756846410464 35.5 30.5 39.0 2.0 40.0 66-67 30.073166594417323 35.0 29.5 38.5 2.0 40.0 68-69 29.45764932054206 35.0 29.0 37.5 2.0 39.0 70-71 28.855433778766233 34.5 28.5 37.0 2.0 39.0 72-73 28.302132612096422 34.0 27.0 36.0 2.0 38.5 74-75 27.86566334319694 34.0 26.0 35.5 2.0 37.5 76-77 26.74170232014626 32.5 26.0 34.5 2.0 36.0 78-79 27.224381325744012 34.0 26.0 35.0 2.0 36.5 80-81 26.88109014833104 33.0 26.0 35.0 2.0 36.0 82-83 26.508740599732363 33.0 24.5 35.0 2.0 36.0 84-85 26.08126166198617 33.0 24.0 35.0 2.0 35.0 86-87 25.85426993610645 33.0 23.0 35.0 2.0 35.0 88-89 25.307928265827318 32.5 19.0 35.0 2.0 35.0 90-91 24.75766628343103 32.0 15.5 34.5 2.0 35.0 92-93 24.360890174717756 32.0 4.5 34.0 2.0 35.0 94-95 23.789815669939877 31.0 2.0 34.0 2.0 35.0 96-97 23.10113651356089 31.0 2.0 34.0 2.0 35.0 98-99 22.40530467233353 31.0 2.0 34.0 2.0 35.0 100-101 20.26750475903274 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1782452278530009 1101 2 0.01418822073365078 1101 3 -0.0647237512309573 1101 4 -0.07390831546196353 1101 5 -0.42777085249775837 1101 6 -0.011388484876860616 1101 7 0.17962014126860737 1101 8 -0.26787804854241415 1101 9 0.34683402310448486 1101 10-11 0.14626762999576925 1101 12-13 0.15185840172934917 1101 14-15 0.6076398228096807 1101 16-17 0.11976533391335664 1101 18-19 0.20149525204402607 1101 20-21 0.3944210827325847 1101 22-23 0.20484717965941712 1101 24-25 0.1262358840442772 1101 26-27 0.14660141463787824 1101 28-29 0.0866640365064697 1101 30-31 0.18457519724261573 1101 32-33 0.34607366819409435 1101 34-35 0.2935936278577955 1101 36-37 0.53724961250105 1101 38-39 1.4266353519400852 1101 40-41 1.7820393096461373 1101 42-43 1.521214032088558 1101 44-45 1.3678605914573296 1101 46-47 1.352998799432278 1101 48-49 1.7870907229089141 1101 50-51 2.5083092431650513 1101 52-53 2.0452606183879922 1101 54-55 2.1277621284473796 1101 56-57 2.7168920464639648 1101 58-59 3.2547270256241028 1101 60-61 2.4582779362146248 1101 62-63 2.0777990008541813 1101 64-65 1.638724907224553 1101 66-67 2.2509271916753697 1101 68-69 2.5121618485693205 1101 70-71 2.7319240079823466 1101 72-73 2.2233689354155644 1101 74-75 2.5582165392547545 1101 76-77 2.7376431643850268 1101 78-79 3.105248114149159 1101 80-81 3.313020082753134 1101 82-83 2.7564171259871593 1101 84-85 3.208333078803939 1101 86-87 2.49076715665635 1101 88-89 2.6713566391810204 1101 90-91 2.9148073365925526 1101 92-93 3.1040472185040464 1101 94-95 3.138591306803807 1101 96-97 3.103189528778561 1101 98-99 3.8881475351272066 1101 100-101 3.8798508995996883 1102 1 0.15903671130590524 1102 2 0.2987982647049563 1102 3 0.18429268015066924 1102 4 0.1533538923603004 1102 5 0.17852398665984026 1102 6 -0.36737089635568765 1102 7 -0.08934537597276915 1102 8 -0.4098359286627584 1102 9 -0.12898176027898955 1102 10-11 -0.21151991918267044 1102 12-13 -0.1735755256022884 1102 14-15 0.42166435416816483 1102 16-17 0.242260126785375 1102 18-19 0.29404327333539726 1102 20-21 0.22888069856225712 1102 22-23 0.24421306718545566 1102 24-25 -0.2196331277585486 1102 26-27 0.16537021815558006 1102 28-29 -0.34513415835584027 1102 30-31 -0.9473155687837647 1102 32-33 -1.4132644479827192 1102 34-35 -0.6136732085226626 1102 36-37 -0.6887165990240547 1102 38-39 -0.7215247961737816 1102 40-41 -1.1928044765144747 1102 42-43 -1.1052150352569683 1102 44-45 -0.3527364925510028 1102 46-47 0.5453385356044507 1102 48-49 0.6016938240429113 1102 50-51 -0.0896310484341214 1102 52-53 0.16615855867959084 1102 54-55 0.5071187588570112 1102 56-57 0.8119857747675958 1102 58-59 0.7634750006298923 1102 60-61 0.8303908730348013 1102 62-63 0.4822887023121787 1102 64-65 0.6957487442993049 1102 66-67 1.0434520701757144 1102 68-69 1.7146381272085236 1102 70-71 1.2517110943049587 1102 72-73 1.7441048761700202 1102 74-75 1.4460665739689418 1102 76-77 1.2538199753084243 1102 78-79 0.4383191625175904 1102 80-81 0.8237583377866926 1102 82-83 0.599671002404655 1102 84-85 0.690351130180936 1102 86-87 1.095696571143737 1102 88-89 0.8374061647538014 1102 90-91 1.3853002780412922 1102 92-93 1.7071946380828464 1102 94-95 1.2096859608190798 1102 96-97 1.9737518986003586 1102 98-99 1.6724798656062596 1102 100-101 2.8791103302870305 1103 1 -0.13061846110788977 1103 2 -0.2943051835709092 1103 3 -0.5467418026079507 1103 4 -0.1983702455707359 1103 5 -0.7387173926505 1103 6 0.04642220709258993 1103 7 -0.2410695139038097 1103 8 -0.08569799762827301 1103 9 -0.14967141545140095 1103 10-11 -0.2528992295274932 1103 12-13 -0.43909276698160227 1103 14-15 0.1733884920991997 1103 16-17 -0.3267053904560058 1103 18-19 -0.35423258873357355 1103 20-21 -0.519395163506708 1103 22-23 -0.3799248638490269 1103 24-25 -0.10584002431027528 1103 26-27 0.034335735396958 1103 28-29 -0.23134105490755275 1103 30-31 -0.4645569480941063 1103 32-33 -0.07188513763789217 1103 34-35 0.3794302397531979 1103 36-37 0.13886960787249336 1103 38-39 0.1750269279641543 1103 40-41 -0.1479768903075822 1103 42-43 0.3327159992257904 1103 44-45 -0.12170200979237222 1103 46-47 0.5453385356044507 1103 48-49 1.5741076171463604 1103 50-51 1.7551965377727825 1103 52-53 1.848917179369245 1103 54-55 1.7243601381673557 1103 56-57 1.9085374989055346 1103 58-59 1.949681897181616 1103 60-61 1.8303908730347977 1103 62-63 2.330564564381138 1103 64-65 1.733679778782065 1103 66-67 1.8744865529343393 1103 68-69 1.6732588168636973 1103 70-71 2.6517110943049573 1103 72-73 2.7268634968596785 1103 74-75 2.739170022244803 1103 76-77 2.5503716994463517 1103 78-79 2.8417674383796587 1103 80-81 2.489275579166005 1103 82-83 2.827257209301205 1103 84-85 2.60414423362921 1103 86-87 2.6819034676954594 1103 88-89 2.5615440957882853 1103 90-91 1.8784037263171527 1103 92-93 2.6416773967035354 1103 94-95 2.4269273401294242 1103 96-97 2.8392691399796703 1103 98-99 2.520755727675226 1103 100-101 3.3239379164939287 1104 1 0.004835642826058972 1104 2 0.2851022957880076 1104 3 0.10561370977670492 1104 4 -0.15723377155421758 1104 5 -0.170043279003103 1104 6 0.023255621371369273 1104 7 -0.24291506999900037 1104 8 -0.3507781336166147 1104 9 -0.0034353785402814196 1104 10-11 -0.26069427566639547 1104 12-13 0.11081009848707879 1104 14-15 -0.08032690372402129 1104 16-17 -0.0548258372748478 1104 18-19 0.20402384642913063 1104 20-21 0.13645719200567186 1104 22-23 -0.01970145442697202 1104 24-25 0.18687488584028955 1104 26-27 0.0665843997971507 1104 28-29 0.40836268477189464 1104 30-31 0.048361944572235416 1104 32-33 -0.1613703246218634 1104 34-35 0.5464530663680591 1104 36-37 0.42218675211727685 1104 38-39 0.2094125520535144 1104 40-41 0.13949275515138027 1104 42-43 0.3137747656172465 1104 44-45 0.3081668585903401 1104 46-47 0.7380777293878467 1104 48-49 0.3862494335620923 1104 50-51 0.6149828418038581 1104 52-53 0.43383704338089757 1104 54-55 0.17890117750683743 1104 56-57 0.010941578555847542 1104 58-59 0.3988805372981723 1104 60-61 0.7455924271873009 1104 62-63 0.4432163370863407 1104 64-65 0.1631601090394703 1104 66-67 0.4743651347701814 1104 68-69 0.7762699873347998 1104 70-71 0.6642900161116607 1104 72-73 0.943522068982066 1104 74-75 1.208159823119015 1104 76-77 0.7867971486935588 1104 78-79 0.8095916248779567 1104 80-81 1.656152703983878 1104 82-83 1.5951542466980015 1104 84-85 1.872502660049804 1104 86-87 2.24341390965758 1104 88-89 2.167906407590131 1104 90-91 2.0812691949912683 1104 92-93 2.106150441871094 1104 94-95 1.2163396762149077 1104 96-97 1.5012895382312514 1104 98-99 1.9508188651205849 1104 100-101 2.529935973803301 1105 1 0.20088983054781195 1105 2 0.1837153481479632 1105 3 0.15270542034545542 1105 4 0.2641725288435879 1105 5 0.038697716588771414 1105 6 0.2040426335206007 1105 7 -0.011219553387881831 1105 8 0.2654996859726708 1105 9 0.37378212495396923 1105 10-11 0.4099594174848775 1105 12-13 0.2331894125393319 1105 14-15 0.3988688816754049 1105 16-17 0.5829550465010911 1105 18-19 0.2903317703082777 1105 20-21 0.1939241310444828 1105 22-23 0.3761161995887221 1105 24-25 0.26799519548441 1105 26-27 0.5397055695638429 1105 28-29 0.10003562316561698 1105 30-31 0.17840172524098108 1105 32-33 0.5772330513592152 1105 34-35 0.47113858405433007 1105 36-37 0.5376850856297963 1105 38-39 0.3375170569454653 1105 40-41 0.7260425885026294 1105 42-43 0.06132879206601416 1105 44-45 0.3645048867593488 1105 46-47 0.6984314547937132 1105 48-49 1.5029573144351147 1105 50-51 0.9632249663066048 1105 52-53 0.8420995957946218 1105 54-55 1.0249392379304467 1105 56-57 1.4367291282816872 1105 58-59 1.5960098782292569 1105 60-61 1.2029626024372782 1105 62-63 1.2757343459025954 1105 64-65 1.6326795155548979 1105 66-67 1.4751446208469439 1105 68-69 1.381366213547036 1105 70-71 1.2064360219174866 1105 72-73 1.941156731921538 1105 74-75 1.7896833152166387 1105 76-77 1.965165066121795 1105 78-79 1.9818042901827653 1105 80-81 2.5687965057256257 1105 82-83 2.6417083806620916 1105 84-85 3.021438258372566 1105 86-87 2.52104534713742 1105 88-89 2.3362216425111058 1105 90-91 2.166690117472722 1105 92-93 3.0586555488488365 1105 94-95 3.225532236154695 1105 96-97 3.197021179990198 1105 98-99 2.7318639140400585 1105 100-101 2.6624217280232756 1106 1 0.1814645217909039 1106 2 0.060895264985305175 1106 3 0.1848533754127928 1106 4 0.09840575667205087 1106 5 0.02696805730744245 1106 6 -0.0033675321841144523 1106 7 0.049538840455596755 1106 8 0.119965024519189 1106 9 0.0423106423002082 1106 10-11 0.12013132836428753 1106 12-13 -0.1263649845313637 1106 14-15 0.300470073259838 1106 16-17 0.3002640516522135 1106 18-19 0.11187338267097147 1106 20-21 0.3962762690696806 1106 22-23 0.08169554545851554 1106 24-25 0.5796379684006965 1106 26-27 -0.12624738767565447 1106 28-29 0.15195022628111587 1106 30-31 0.545900018544522 1106 32-33 0.6068364771644639 1106 34-35 0.3314066905521855 1106 36-37 0.7285247802862855 1106 38-39 1.1571968186285773 1106 40-41 1.4890289969926727 1106 42-43 1.7739831705181999 1106 44-45 2.153122772938204 1106 46-47 1.9630004363613978 1106 48-49 2.275004729565758 1106 50-51 2.160270829895012 1106 52-53 2.6179948356630476 1106 54-55 2.384214357399202 1106 56-57 2.47198016781498 1106 58-59 2.781108866623722 1106 60-61 3.014915683800151 1106 62-63 2.925209924627847 1106 64-65 3.3277083742404336 1106 66-67 3.516819045224782 1106 68-69 3.1266650405811056 1106 70-71 3.9262274946413704 1106 72-73 4.179008156237309 1106 74-75 4.0606446507841945 1106 76-77 4.042493931013496 1106 78-79 4.5558128546958905 1106 80-81 4.578229882502143 1106 82-83 4.309399065202523 1106 84-85 4.064447009070758 1106 86-87 3.8598401091308396 1106 88-89 4.380719873752959 1106 90-91 4.812690241596101 1106 92-93 5.307026429504212 1106 94-95 4.065475139400526 1106 96-97 5.206888988591949 1106 98-99 5.347360717863058 1106 100-101 5.24815995181773 1107 1 -0.07075854731478515 1107 2 -0.18573837322608 1107 3 0.06468061118515323 1107 4 -0.06216334901900922 1107 5 -0.3278337719608473 1107 6 0.1351614312305216 1107 7 -0.06256735873139263 1107 8 -0.00471739417999828 1107 9 -0.34234382924450557 1107 10-11 -0.3526837122861153 1107 12-13 0.0933695175011593 1107 14-15 0.0914380610647143 1107 16-17 -0.01988965769738371 1107 18-19 -0.13383926976805327 1107 20-21 -0.2961462410929201 1107 22-23 -0.1791974931593714 1107 24-25 0.3475813118966258 1107 26-27 0.24357603712109466 1107 28-29 -0.24112014973514562 1107 30-31 0.17655296569899548 1107 32-33 0.6025491296034886 1107 34-35 0.23279769664974737 1107 36-37 0.7636810302862855 1107 38-39 1.031815721067602 1107 40-41 0.389657807968284 1107 42-43 0.48317397336372636 1107 44-45 0.2902861367593488 1107 46-47 0.30646461319066276 1107 48-49 0.7538220568015319 1107 50-51 1.1602073136348494 1107 52-53 0.6744290328175211 1107 54-55 0.6768655260983891 1107 56-57 1.2463068954572556 1107 58-59 1.214956681664372 1107 60-61 1.6801214764830767 1107 62-63 1.1103598230018328 1107 64-65 1.4583295632648223 1107 66-67 1.2015878460377856 1107 68-69 1.2427092478981798 1107 70-71 1.4052671287877168 1107 72-73 0.9560660830665739 1107 74-75 0.9244340308654948 1107 76-77 1.0418587684118705 1107 78-79 1.4142351107934523 1107 80-81 0.865164587786694 1107 82-83 1.4033078558529297 1107 84-85 1.160959966387832 1107 86-87 1.187075881488564 1107 88-89 1.128623837167595 1107 90-91 0.7344382090757762 1107 92-93 0.36495325877250195 1107 94-95 0.616097598750116 1107 96-97 0.7445223727382917 1107 98-99 0.6475068052614326 1107 100-101 1.3207590371835813 1108 1 0.22365305178431427 1108 2 0.13626331596310592 1108 3 0.3048270075815509 1108 4 0.17882764197198497 1108 5 0.03773492173284865 1108 6 0.16099194924854032 1108 7 0.3507913124397817 1108 8 0.2812482589731573 1108 9 0.08164265724197861 1108 10-11 0.016967188614792406 1108 12-13 0.5186580873209792 1108 14-15 0.20961092142507454 1108 16-17 -0.047022259048738135 1108 18-19 0.04144930005176661 1108 20-21 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-3.180115437937335 2316 78-79 -3.182863103492263 2316 80-81 -2.520686106657749 2316 82-83 -3.4246311322423075 2316 84-85 -3.066408585199472 2316 86-87 -4.126416182003499 2316 88-89 -3.2427799326736775 2316 90-91 -3.70802210838454 2316 92-93 -3.6370308682116246 2316 94-95 -2.8116057742657574 2316 96-97 -3.956680504245835 2316 98-99 -3.2641375399766623 2316 100-101 -4.00600187551483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 8.0 6 12.0 7 38.0 8 59.0 9 98.0 10 241.0 11 344.0 12 622.0 13 756.0 14 1013.0 15 1522.0 16 2062.0 17 2869.0 18 2433.0 19 1292.0 20 1075.0 21 1064.0 22 1083.0 23 1142.0 24 1170.0 25 1278.0 26 1340.0 27 1574.0 28 1901.0 29 2246.0 30 2742.0 31 3343.0 32 4160.0 33 5649.0 34 7846.0 35 11780.0 36 14484.0 37 17964.0 38 10320.0 39 582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.34907047777065 22.01723292733512 16.839812964289756 13.793883630604473 2 15.378743615357068 28.168761897581845 35.099986806641915 21.352507680419173 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21.110786512618503 32.065043255366874 33.37778238498219 13.446387847032437 48-49 19.333923893171495 30.711310477411086 33.84096349209341 16.11380213732401 50-51 13.963284769210471 32.89292647530015 37.05731571705901 16.08647303843037 52-53 17.796426484723977 35.45903462314115 29.522965866897867 17.221573025237007 54-55 15.764178148029478 31.366737659498273 31.722487136475863 21.14659705599638 56-57 19.964849124526452 30.632150328891573 30.462050247846655 18.940950298735324 58-59 23.087905460165484 30.111483875831652 30.044103511318017 16.75650715268485 60-61 18.078198918144636 32.472623781970334 34.61371732287917 14.835459977005861 62-63 15.745330493620068 32.02593437246735 34.190587481387944 18.038147652524643 64-65 15.569576116252332 36.10221082986222 31.197579961173833 17.130633092711612 66-67 18.311909832821303 31.157999886914073 28.907118759070432 21.622971521194188 68-69 16.704676103058976 31.29323180730158 31.28569274553782 20.716399344101628 70-71 21.32989049512788 31.06093446670562 29.82924025105076 17.779934787115746 72-73 18.82503722411746 28.42697476299075 35.60745990161524 17.140528111276552 74-75 15.81223966677347 35.70358293910323 30.594926211432988 17.889251182690312 76-77 17.248430932770418 30.863034095406828 30.648170835139567 21.24036413668319 78-79 17.492508057372262 31.50008481444484 31.49348813540155 19.51391899278135 80-81 19.36266656614584 29.705316923308896 31.092975479201613 19.839041031343648 82-83 17.276783821097222 29.351590513930837 34.09368270766679 19.27794295730515 84-85 16.0323615751059 31.381492458546745 32.82522958907208 19.760916377275276 86-87 15.68219085134855 32.37555836176188 31.02606630604821 20.91618448084136 88-89 15.429632282262473 32.26765554026802 28.740317017547167 23.562395159922346 90-91 17.655208161534294 27.973988643592584 30.61235068209128 23.75845251278185 92-93 19.21911592161002 30.547401058330703 31.37625399936858 18.857229020690696 94-95 17.834121793542792 29.555006879393858 31.665944173247638 20.944927153815705 96-97 13.681041144429576 30.332001432421734 34.59581205119023 21.39114537195846 98-99 16.95346514126317 31.861017396385023 28.647021128220594 22.538496334131217 100-101 15.085625960170027 28.849869464001284 31.079348922253324 24.985155653575365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2174.0 1 1317.5 2 475.0 3 434.0 4 318.0 5 330.0 6 569.0 7 957.0 8 1178.0 9 1115.0 10 876.0 11 666.0 12 612.5 13 594.5 14 556.0 15 525.5 16 516.0 17 527.5 18 555.5 19 569.0 20 622.0 21 668.5 22 714.0 23 777.5 24 842.0 25 1005.0 26 1309.0 27 1704.5 28 1801.5 29 1797.5 30 1909.0 31 2017.0 32 2268.5 33 2482.0 34 2731.5 35 2991.5 36 3316.5 37 3555.0 38 3129.0 39 2515.0 40 2224.5 41 1993.5 42 1719.0 43 1674.5 44 1697.5 45 1664.0 46 2051.0 47 5772.0 48 12245.0 49 11119.5 50 4844.0 51 2002.5 52 974.0 53 631.5 54 509.0 55 456.0 56 388.5 57 336.0 58 337.5 59 335.5 60 312.5 61 292.5 62 216.5 63 112.0 64 61.0 65 55.5 66 43.5 67 30.5 68 26.0 69 16.0 70 11.5 71 11.0 72 5.5 73 2.5 74 3.0 75 2.0 76 1.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03581054337787662 2 0.0 3 0.0 4 0.0 5 0.019790037129879187 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0042407222421169686 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.7119136023521875E-4 26-27 0.0014135740807056563 28-29 0.0023559568011760935 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009423827204704374 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.7119136023521875E-4 84-85 4.7119136023521875E-4 86-87 0.0 88-89 0.0 90-91 0.0061254876830578436 92-93 0.0032983395216465315 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012250975366115687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 106114.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.75897619541248 #Duplication Level Percentage of deduplicated Percentage of total 1 79.56340898305558 26.859792298848404 2 9.519024090668006 6.427050153608384 3 3.4893783323563077 3.5339352017641406 4 1.8926388074700613 2.5557419379158266 5 1.1975546436646847 2.0214109354090883 6 0.7537057197889624 1.5266600071621088 7 0.5275940038522737 1.2467723391823888 8 0.3991848812215616 1.0780858322181806 9 0.30427379058146997 0.9244774487814992 >10 2.0405884487619685 12.714627664587141 >50 0.15353264662367752 3.6272310910907137 >100 0.09491109064009157 6.369564807659686 >500 0.025123523992965417 6.212186893341124 >1k 0.0362895346565056 19.99076464933939 >5k 0.002791502665885046 4.9116987390919205 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5212 4.9116987390919205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2355 2.2193113067078802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2082 1.962040824019451 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2058 1.93942363872816 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1823 1.7179636994176077 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1813 1.7085398722129033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1692 1.5945115630359803 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1615 1.5219480935597565 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1534 1.445615093201651 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1374 1.2948338579263812 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1315 1.2392332774186252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1261 1.1883446105132216 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1205 1.1355711781668771 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1086 1.023427634430895 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 945 0.8905516708445634 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 918 0.8651073373918615 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 872 0.8217577322502214 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 723 0.6813427069001262 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 722 0.6804003241796559 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 675 0.6361083363175453 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 619 0.5833349039712008 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 599 0.5644872495617921 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 519 0.4890966319241571 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.4240722242116969 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 402 0.3788378536291158 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 389 0.3665868782630002 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 363 0.3420849275307688 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 300 0.28271481614113125 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.27517575437736774 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 259 0.2440771246018433 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 255 0.24030759371996155 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 243 0.22899900107431628 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.19978513673973275 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.196015605857851 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 202 0.19036130953502836 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.1894189268145579 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 194 0.18282224777126488 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 190 0.1790527168893831 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 190 0.1790527168893831 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 174 0.1639745933618561 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 167 0.15737791431856307 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 160 0.15078123527527 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 159 0.14983885255479956 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 156 0.14701170439338826 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.14041502535009517 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 149 0.14041502535009517 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 132 0.12439451910209774 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.11874022277927512 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 126 0.11874022277927512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 123 0.11591307461786382 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 118 0.11120116101551161 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 116 0.10931639557457075 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 116 0.10931639557457075 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 115 0.10837401285410031 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 111 0.10460448197221857 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 111 0.10460448197221857 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.423827204704375E-4 0.0 20-21 0.0 0.0 0.0 0.0014135740807056563 0.0 22-23 0.0 0.0 0.0 0.0061254876830578436 0.0 24-25 0.0 0.0 0.0 0.01130859264564525 0.0 26-27 0.0 0.0 0.0 0.013664549446821342 0.0 28-29 0.0 0.0 0.0 0.03392577793693575 0.0 30-31 0.0 0.0 0.0 0.05889892002940234 0.0 32-33 0.0 0.0 0.0 0.09423827204704374 0.0 34-35 0.0 0.0 0.0 0.12675047590327385 0.0 36-37 0.0 0.0 0.0 0.15973387111973913 0.0 38-39 0.0 0.0 0.0 0.23512448875737413 0.0 40-41 0.0 0.0 0.0 0.3500951806547675 0.0 42-43 0.0 0.0 0.0 0.44056392181992954 0.0 44-45 0.0 0.0 0.0 0.5607177186799103 0.0 46-47 0.0 0.0 0.0 0.7817064666302278 0.0 48-49 0.0 0.0 0.0 0.913168856135854 0.0 50-51 0.0 0.0 0.0 1.0752586840567693 0.0 52-53 0.0 0.0 0.0 1.2439451910209773 0.0 54-55 0.0 0.0 0.0 1.4588084512882373 0.0 56-57 0.0 0.0 0.0 1.9304710028836913 0.0 58-59 0.0 0.0 0.0 2.221196072148821 0.0 60-61 0.0 0.0 0.0 2.473283449874663 0.0 62-63 0.0 0.0 0.0 2.736208228885915 0.0 64-65 0.0 0.0 0.0 3.003373730139284 0.0 66-67 0.0 0.0 0.0 3.4411105037978027 0.0 68-69 0.0 0.0 0.0 3.95282432101325 0.0 70-71 0.0 0.0 0.0 4.341086001847071 0.0 72-73 0.0 0.0 0.0 4.723222194997833 0.0 74-75 0.0 0.0 0.0 5.343781216427615 0.0 76-77 0.0 0.0 0.0 5.902614169666585 0.0 78-79 0.0 0.0 0.0 6.759240062574213 0.0 80-81 0.0 0.0 0.0 7.546129634167029 0.0 82-83 0.0 0.0 0.0 8.212394217539627 0.0 84-85 0.0 0.0 0.0 8.996456640971031 0.0 86-87 0.0 0.0 0.0 9.618900427841755 0.0 88-89 0.0 0.0 0.0 10.478353468910793 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 30 1.295812E-4 63.333336 2 ATGGGTC 25 0.0050087557 57.0 7 GCTAAAG 25 0.0050087557 57.0 1 TTGCGTT 40 5.365963E-4 47.499996 4 GCTTTGC 40 5.365963E-4 47.499996 1 ATGGGTA 45 9.585562E-4 42.22222 5 CATGGGT 90 1.1790689E-8 42.22222 4 CGCAGAA 80 2.2367567E-7 41.5625 9 TTTGCGT 50 0.0016092121 38.0 3 TGTCGTA 30 0.003943759 31.666668 64-65 ATTTATC 65 0.005820508 29.230766 2 TGTCAGT 65 0.005820508 29.230766 1 CTGCGGC 35 0.008376428 27.142859 14-15 GTTGTCG 35 0.008376428 27.142859 62-63 TACTGAA 35 0.008376428 27.142859 90-91 CTATTAA 70 0.008357883 27.142859 1 GGCGAAT 35 0.008376428 27.142859 92-93 AAGGCGA 35 0.008376428 27.142859 90-91 GGTTAAC 70 0.008357883 27.142859 7 AAGCAGG 45 8.923428E-4 26.388887 94-95 >>END_MODULE