FastQCFastQC Report
Thu 26 May 2016
SRR937727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886053
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.491184500117441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73170.3879530426769555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.26759587349878294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26970.14299704197071875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.039.3361241
GTATCAA100450.033.0534251
ATCAACG124500.026.541073
TCAACGC125550.026.2440624
TATCAAC126650.026.203832
GTACATG87150.026.0898461
CAACGCA128050.025.7316845
TACATGG87250.025.3397352
AACGCAG131000.025.0912386
ACATGGG87400.024.4795863
GAGTACT82000.022.64966212-13
CGCAGAG147450.022.0348538
ACGCAGA147750.021.9906967
CATGGGG43900.021.2278564
GTACTTT87400.021.11438614-15
AGAGTAC127350.020.55160310-11
GTACCTG14800.020.2483581
GCAGAGT156750.020.242139
AGTACTT87100.020.09639712-13
CATGGGA44200.019.255084
Produced by FastQC (version 0.11.5)