##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937727_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886053 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43553601091804 31.0 31.0 34.0 30.0 34.0 2 31.57724358753439 33.0 31.0 34.0 30.0 34.0 3 31.58581015485779 33.0 31.0 34.0 28.0 34.0 4 35.201309825333645 37.0 35.0 37.0 33.0 37.0 5 35.18550380079457 37.0 35.0 37.0 33.0 37.0 6 35.24439451065267 37.0 35.0 37.0 33.0 37.0 7 35.17049626919286 37.0 35.0 37.0 32.0 37.0 8 35.156921359049825 37.0 35.0 37.0 32.0 37.0 9 36.73861126914249 39.0 37.0 39.0 33.0 39.0 10-11 36.59447852207759 39.0 37.0 39.0 32.0 39.0 12-13 36.45490211568816 39.0 37.0 39.0 32.0 39.0 14-15 37.53431929007297 40.0 37.0 41.0 32.0 41.0 16-17 37.43648587818052 40.0 37.0 41.0 32.0 41.0 18-19 37.3630452060467 40.0 37.0 41.0 32.0 41.0 20-21 37.25697607649414 40.0 37.0 41.0 31.5 41.0 22-23 37.16568065690625 39.5 37.0 41.0 31.0 41.0 24-25 37.1910285660053 40.0 37.0 41.0 31.0 41.0 26-27 37.094390507583825 39.5 36.5 41.0 31.0 41.0 28-29 36.95921906754476 39.0 36.0 41.0 31.0 41.0 30-31 36.70067251556557 39.0 36.0 41.0 30.0 41.0 32-33 36.53628821671501 39.0 36.0 41.0 30.0 41.0 34-35 36.518146891948426 39.0 35.5 41.0 30.0 41.0 36-37 36.349836669489136 39.0 35.0 41.0 30.0 41.0 38-39 36.18596534667901 39.0 35.0 41.0 30.0 41.0 40-41 35.93732890857256 39.0 35.0 40.5 28.5 41.0 42-43 35.640927375847866 39.0 35.0 40.0 27.0 41.0 44-45 35.370197974288104 38.5 35.0 40.0 26.5 41.0 46-47 34.974382480237836 38.0 34.5 40.0 24.5 41.0 48-49 34.617827547794256 38.0 34.0 40.0 24.0 41.0 50-51 33.71090128432233 37.5 33.0 39.5 22.5 40.5 52-53 33.79858386800371 38.0 33.0 39.5 23.0 40.5 54-55 34.05996013897807 38.0 34.0 40.0 22.0 41.0 56-57 33.79149286897028 38.0 33.0 40.0 20.5 41.0 58-59 33.513818275520364 38.0 33.0 40.0 19.5 41.0 60-61 33.15404948853505 37.0 32.5 40.0 17.5 41.0 62-63 32.749370775900786 37.0 32.0 39.5 14.0 41.0 64-65 32.314705896387856 36.0 31.0 39.0 10.0 41.0 66-67 31.8483839001343 35.5 31.0 39.0 8.0 40.0 68-69 31.30506539317824 35.0 30.5 38.5 7.0 40.0 70-71 30.81361446364445 35.0 30.0 38.0 2.0 40.0 72-73 30.32415817583069 35.0 29.0 37.0 2.0 39.0 74-75 29.770437257065417 34.0 29.0 36.5 2.0 39.0 76-77 29.251720656842622 34.0 28.5 36.0 2.0 39.0 78-79 28.67318362739541 34.0 27.5 35.5 2.0 37.5 80-81 28.082783198563348 33.5 26.0 35.0 2.0 37.0 82-83 27.543114907163265 33.0 26.0 35.0 2.0 36.5 84-85 26.9956321481952 33.0 25.5 35.0 2.0 36.0 86-87 26.42820535796184 32.0 24.5 35.0 2.0 36.0 88-89 25.9029831081099 32.0 23.5 35.0 2.0 35.0 90-91 25.292503710129036 32.0 20.0 34.0 2.0 35.0 92-93 24.598037806996942 31.0 17.5 34.0 2.0 35.0 94-95 23.795068060123445 31.0 4.5 34.0 2.0 35.0 96-97 22.905059136726273 30.5 2.0 34.0 2.0 35.0 98-99 21.784772485184668 30.0 2.0 34.0 2.0 35.0 100-101 19.015003289939358 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4790087309844395 1101 2 0.5441177671499666 1101 3 0.470970377277375 1101 4 0.36517675007104344 1101 5 0.267473308473555 1101 6 0.4003836686406359 1101 7 0.41635945390034834 1101 8 0.3921616602805358 1101 9 0.5383578721189011 1101 10-11 0.4542389776690925 1101 12-13 0.5045186891971056 1101 14-15 0.6703635549679419 1101 16-17 0.6956349273460631 1101 18-19 0.7463150665870657 1101 20-21 0.7337796332239961 1101 22-23 0.773371198232411 1101 24-25 0.6733734318139071 1101 26-27 0.7616932675995756 1101 28-29 0.6771386720984438 1101 30-31 0.8462400353513786 1101 32-33 0.8323698528011505 1101 34-35 0.9515214615418657 1101 36-37 0.9452742253607909 1101 38-39 1.0189077918150105 1101 40-41 1.0018999470788614 1101 42-43 1.0639197588794502 1101 44-45 1.097695339940742 1101 46-47 1.1292787973415557 1101 48-49 1.2273157704569897 1101 50-51 1.0675127966327622 1101 52-53 1.044858977873929 1101 54-55 1.1606142488307185 1101 56-57 1.0667729410663895 1101 58-59 1.0723586767837858 1101 60-61 1.2387466089910717 1101 62-63 1.3371893975937112 1101 64-65 1.445298726792835 1101 66-67 1.3955290565167182 1101 68-69 1.3555234348098217 1101 70-71 1.414623334328521 1101 72-73 1.3266761698329574 1101 74-75 1.3979937837972756 1101 76-77 1.3112921736538716 1101 78-79 1.377803436663168 1101 80-81 1.5634502770693217 1101 82-83 1.5438181048245383 1101 84-85 1.5825123736834108 1101 86-87 1.6366519002519446 1101 88-89 1.6064854152693755 1101 90-91 1.8096657815031172 1101 92-93 1.7245287802392184 1101 94-95 1.8953980066910603 1101 96-97 2.247283783471559 1101 98-99 2.622541698910645 1101 100-101 2.6806308314893386 1102 1 0.19701905119703866 1102 2 0.3253321201107724 1102 3 0.31728977951540926 1102 4 0.21701496345871618 1102 5 0.2930340678314636 1102 6 0.221779638275855 1102 7 0.19701322625981987 1102 8 0.1662541131781765 1102 9 0.41844772708773803 1102 10-11 0.23293500808154732 1102 12-13 0.4241257822577751 1102 14-15 0.4191258444820747 1102 16-17 0.608847897922729 1102 18-19 0.5550795011800389 1102 20-21 0.5907253984808776 1102 22-23 0.5749284062931395 1102 24-25 0.8055127904353441 1102 26-27 0.5914400291263959 1102 28-29 0.7046831526914374 1102 30-31 0.6304763277017926 1102 32-33 0.7933102315267391 1102 34-35 0.6994403775620199 1102 36-37 0.8031944522624599 1102 38-39 0.8879587136030551 1102 40-41 0.7811583456583477 1102 42-43 0.6242318678494598 1102 44-45 0.5858902561039088 1102 46-47 0.7802785232481497 1102 48-49 0.8288261442965634 1102 50-51 0.6852801270821587 1102 52-53 0.6779545649700935 1102 54-55 0.5791205589376247 1102 56-57 0.5506190674115388 1102 58-59 0.5732214751582987 1102 60-61 0.6242254978402357 1102 62-63 0.713024488699979 1102 64-65 0.6325637512824898 1102 66-67 0.7456341653794745 1102 68-69 0.6505203959481456 1102 70-71 0.7316494208705357 1102 72-73 0.8460897291145955 1102 74-75 0.8142830357606172 1102 76-77 0.8655169064128039 1102 78-79 0.9610944642750994 1102 80-81 1.0388792332501282 1102 82-83 0.9785563456215556 1102 84-85 0.942058212826332 1102 86-87 1.172841286878569 1102 88-89 1.332007920721452 1102 90-91 1.5269156712698972 1102 92-93 1.6453698894833195 1102 94-95 1.6849961619232658 1102 96-97 1.800701013221417 1102 98-99 2.0426729062325144 1102 100-101 2.2734309935619628 1103 1 0.28907071107395765 1103 2 0.2866022365477434 1103 3 0.21617741639385812 1103 4 0.231848397915968 1103 5 0.24862737631720222 1103 6 0.2339838508094445 1103 7 0.2596060435722336 1103 8 0.2151853778050281 1103 9 0.2614178810850234 1103 10-11 0.28304797794661596 1103 12-13 0.40969164282233805 1103 14-15 0.476322111564798 1103 16-17 0.4039627458034616 1103 18-19 0.3592486959447072 1103 20-21 0.3086335779613876 1103 22-23 0.3554450050623146 1103 24-25 0.44259579872750265 1103 26-27 0.40152988495257347 1103 28-29 0.3668518273902279 1103 30-31 0.27969362855990454 1103 32-33 0.34970328575805354 1103 34-35 0.4923721044696592 1103 36-37 0.39955341328641225 1103 38-39 0.4780560814918786 1103 40-41 0.5226099073005983 1103 42-43 0.5127256992903284 1103 44-45 0.3665409162986393 1103 46-47 0.5030984359926549 1103 48-49 0.5043027268859177 1103 50-51 0.32724886534455777 1103 52-53 0.45088513617577064 1103 54-55 0.5732628836320828 1103 56-57 0.4217594563052316 1103 58-59 0.4827906872584933 1103 60-61 0.6263872383273679 1103 62-63 0.730108652694085 1103 64-65 0.7584358728623357 1103 66-67 0.7616481493729985 1103 68-69 0.6739794118679363 1103 70-71 0.7837218833824515 1103 72-73 0.6990786632684767 1103 74-75 0.6933370937190695 1103 76-77 0.8335900623458876 1103 78-79 1.0281425125834396 1103 80-81 1.121926819277693 1103 82-83 1.10859939551899 1103 84-85 1.1712154171833298 1103 86-87 1.0950099615773645 1103 88-89 1.0809565208774714 1103 90-91 1.248383412739372 1103 92-93 1.1439980712752345 1103 94-95 1.205001362582017 1103 96-97 1.4700200158506362 1103 98-99 1.7539398444891354 1103 100-101 1.7780861609942846 1104 1 0.29476919556230996 1104 2 0.23004902402237093 1104 3 0.16872642418622874 1104 4 0.18437888167228778 1104 5 0.17393176751806294 1104 6 0.15909876120778677 1104 7 0.21104048466524006 1104 8 0.13319710151122166 1104 9 0.2535275344512087 1104 10-11 0.19323887594947564 1104 12-13 0.29564540191836386 1104 14-15 0.43009895049383573 1104 16-17 0.42603123340518323 1104 18-19 0.34862371059097796 1104 20-21 0.37509268615180247 1104 22-23 0.28981468009938993 1104 24-25 0.4259659011564949 1104 26-27 0.4649516091832453 1104 28-29 0.473087285742956 1104 30-31 0.39160882418376275 1104 32-33 0.4342541280995391 1104 34-35 0.4317082297992627 1104 36-37 0.4834747731738389 1104 38-39 0.685963501484224 1104 40-41 0.7857447321536029 1104 42-43 0.5985321588225645 1104 44-45 0.7771694725509022 1104 46-47 0.568221023822808 1104 48-49 0.5878434513096309 1104 50-51 0.605864427989836 1104 52-53 0.5955189126352991 1104 54-55 0.7453476076629997 1104 56-57 0.8306214221071926 1104 58-59 0.7591886103346397 1104 60-61 0.7104300087643409 1104 62-63 0.5751890146492897 1104 64-65 0.6675479131509974 1104 66-67 0.7999290741910805 1104 68-69 0.4834422929358162 1104 70-71 0.5076968960200858 1104 72-73 0.6037287542705911 1104 74-75 0.6348624030202572 1104 76-77 0.6705428360971055 1104 78-79 0.747732127071469 1104 80-81 0.9184237678333211 1104 82-83 0.9482791082402038 1104 84-85 0.8947182900827748 1104 86-87 1.035400841056191 1104 88-89 0.9611507554386307 1104 90-91 1.2026373013920626 1104 92-93 1.132868361734353 1104 94-95 1.066643551855261 1104 96-97 1.21927844066035 1104 98-99 1.3727508915100621 1104 100-101 1.6542707228840925 1105 1 0.0781296877731954 1105 2 0.14744617333860788 1105 3 0.1452501283735259 1105 4 0.15540171943785452 1105 5 0.20644037964588335 1105 6 0.06346616048409004 1105 7 0.04164548664464007 1105 8 0.021407862503835418 1105 9 0.06783677581998404 1105 10-11 0.2635787771878171 1105 12-13 0.19777002340145344 1105 14-15 0.1482322327216039 1105 16-17 0.16422091986122211 1105 18-19 0.17832116185257973 1105 20-21 0.23680096942135265 1105 22-23 0.30620268250245175 1105 24-25 0.2407715118883189 1105 26-27 0.16555743157199743 1105 28-29 0.13238354380813888 1105 30-31 0.1890652730353608 1105 32-33 0.01758666562132305 1105 34-35 0.11292558705672207 1105 36-37 0.15625797501017047 1105 38-39 0.16803603782531695 1105 40-41 0.10282014209783341 1105 42-43 0.08890840983831083 1105 44-45 0.060927906706318424 1105 46-47 0.2057676439099012 1105 48-49 0.33859473357906467 1105 50-51 0.23137772643479337 1105 52-53 0.27278463150489074 1105 54-55 0.26152660101638503 1105 56-57 0.24094612189814058 1105 58-59 0.3885980935722486 1105 60-61 0.3899670461229192 1105 62-63 0.25930775609793244 1105 64-65 0.2657070708293361 1105 66-67 0.4246151265375353 1105 68-69 0.4897196182103265 1105 70-71 0.4041962874412981 1105 72-73 0.4810828733332606 1105 74-75 0.49747389621736815 1105 76-77 0.43526245577443134 1105 78-79 0.45134014850455273 1105 80-81 0.572491218504581 1105 82-83 0.5202374731166124 1105 84-85 0.4317364406630517 1105 86-87 0.6430043645624401 1105 88-89 0.83993657563963 1105 90-91 0.740999469147976 1105 92-93 1.0134036289186739 1105 94-95 1.2112577380786362 1105 96-97 1.367985449473892 1105 98-99 1.4480328909474913 1105 100-101 1.07080579126022 1106 1 -0.02273432630514094 1106 2 -9.249237201309768E-4 1106 3 -0.18205476107356233 1106 4 -0.030722758466161793 1106 5 -0.025839390777925075 1106 6 -0.09420956314823314 1106 7 0.03401485311950836 1106 8 0.10227797378695413 1106 9 0.019407810081162324 1106 10-11 -0.20525245802554792 1106 12-13 -0.2127895503053523 1106 14-15 -0.2253644061486213 1106 16-17 -0.24409966532438432 1106 18-19 -0.16150444713769474 1106 20-21 -0.20487123462689993 1106 22-23 0.06102476825763148 1106 24-25 -0.135038101979724 1106 26-27 -0.006879825635117243 1106 28-29 -0.024880070848517732 1106 30-31 0.045316824719215276 1106 32-33 0.06535205832512503 1106 34-35 0.2918502518930097 1106 36-37 -9.230353003175651E-4 1106 38-39 0.06357302996832459 1106 40-41 0.1727326271286458 1106 42-43 0.0545988543866045 1106 44-45 0.08951768165739082 1106 46-47 0.327711739479021 1106 48-49 0.3595292306144344 1106 50-51 0.2737268297440707 1106 52-53 0.35002562808752913 1106 54-55 0.3250653164412185 1106 56-57 0.43138428090739467 1106 58-59 0.2609168751535762 1106 60-61 0.2370814618135455 1106 62-63 0.4219415660111778 1106 64-65 0.3346310419716616 1106 66-67 0.36908994544690543 1106 68-69 0.08624576317045651 1106 70-71 0.052211866347157354 1106 72-73 0.2566112673222207 1106 74-75 0.33653552909992257 1106 76-77 0.21786972276628092 1106 78-79 0.34203132325701446 1106 80-81 0.10713849338263515 1106 82-83 0.05559487154521037 1106 84-85 0.31213766959666245 1106 86-87 0.31658530404311946 1106 88-89 0.31631355814393913 1106 90-91 0.16751902401551533 1106 92-93 0.17505029407625017 1106 94-95 0.3301509493705126 1106 96-97 0.4591925134689845 1106 98-99 0.4713305112121198 1106 100-101 0.4705989609304595 1107 1 0.1492573096542742 1107 2 0.04862903756173509 1107 3 0.11661801538944516 1107 4 0.09951012010814964 1107 5 0.09827314020085964 1107 6 0.16407441204266604 1107 7 0.06217320012292049 1107 8 0.24758549006536867 1107 9 0.04651646254335873 1107 10-11 0.07111814978250663 1107 12-13 0.16046879464987285 1107 14-15 0.3066312610102244 1107 16-17 0.38144496554622265 1107 18-19 0.391768598196073 1107 20-21 0.33832963082414125 1107 22-23 0.24822440665997902 1107 24-25 0.18222962924634345 1107 26-27 0.24917116298629338 1107 28-29 0.1964902669715869 1107 30-31 0.41507137774019043 1107 32-33 0.37685671049685254 1107 34-35 0.4888357898486575 1107 36-37 0.3603080445956408 1107 38-39 0.30118570338903794 1107 40-41 0.36025043236865173 1107 42-43 0.3545487204167159 1107 44-45 0.4030276926244909 1107 46-47 0.29018085378819336 1107 48-49 0.2568448986670475 1107 50-51 0.10031961901754727 1107 52-53 0.11723270512347028 1107 54-55 0.21331381149112616 1107 56-57 0.12996484251957696 1107 58-59 0.19851637502416963 1107 60-61 0.29840224287938355 1107 62-63 0.38406411819799047 1107 64-65 0.3358925374070054 1107 66-67 0.22479721147441012 1107 68-69 0.30457012483514845 1107 70-71 0.3817645149289781 1107 72-73 0.4581740320733374 1107 74-75 0.36441853494097387 1107 76-77 0.4011105464343565 1107 78-79 0.39898551468033716 1107 80-81 0.5205235650970224 1107 82-83 0.5454342473330627 1107 84-85 0.4890803260174401 1107 86-87 0.5527644810825976 1107 88-89 0.6355075137653685 1107 90-91 0.7102185497677134 1107 92-93 0.5826569253527722 1107 94-95 0.7982784060281354 1107 96-97 1.0905351356292243 1107 98-99 1.1242272491990484 1107 100-101 1.1409411101462794 1108 1 -0.07575849886906028 1108 2 0.0284935150917498 1108 3 0.0922858827592421 1108 4 -0.027300679563779795 1108 5 0.05608843190972124 1108 6 0.029894127858526076 1108 7 0.04646565985620299 1108 8 0.165979088787374 1108 9 0.15266494093594218 1108 10-11 0.07308002514067624 1108 12-13 0.11587747518183988 1108 14-15 0.19699123031866606 1108 16-17 0.24649876011611127 1108 18-19 0.258927764381383 1108 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-1.5479245865506002 2316 96-97 -1.941455406481552 2316 98-99 -1.913319863319952 2316 100-101 -1.8327625281408686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9231.0 3 2020.0 4 1987.0 5 2261.0 6 2524.0 7 3066.0 8 3784.0 9 4825.0 10 5851.0 11 7293.0 12 9206.0 13 11397.0 14 12881.0 15 15400.0 16 19131.0 17 23667.0 18 20978.0 19 15878.0 20 16409.0 21 17290.0 22 18786.0 23 20827.0 24 23203.0 25 26240.0 26 29586.0 27 34690.0 28 40555.0 29 47740.0 30 56963.0 31 68906.0 32 85760.0 33 108301.0 34 140864.0 35 189431.0 36 256879.0 37 314732.0 38 199672.0 39 17836.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94643768347131 18.81827048373906 14.693720964509058 22.541570868280566 2 15.098487125005652 19.820125040221892 41.02813803887363 24.05324979589883 3 19.355101202882835 24.454841501026735 29.697170510250594 26.492886785839843 4 12.508004552755587 16.219351338701614 36.89614824112585 34.37649586741695 5 13.476881341507202 37.68236279737762 34.054389021130596 14.786366839984574 6 32.15232245518209 35.7190525275496 17.338979628704717 14.789645388563596 7 28.652995512907363 33.0220263380914 20.20339361286672 18.12158453613451 8 26.469023920206112 35.21103860739014 19.38403304058502 18.935904431818727 9 26.516114737975776 16.846315572470495 20.38614375348348 36.25142593607025 10-11 25.081969173055185 26.82387886017617 26.716357834860776 21.37779413190787 12-13 25.80892902825444 24.11253107791588 27.77538838927203 22.303151504557654 14-15 22.85575157481319 25.323091734462267 26.356044859860756 25.46511183086379 16-17 21.953724665238557 28.84433343809919 26.867048411123527 22.334893485538725 18-19 21.766829097495425 27.136822580957947 29.724841662484803 21.371506659061826 20-21 22.481634092903757 26.00560911909523 30.048871269707355 21.463885518293655 22-23 22.252568974150215 25.8578587299964 29.982906709415385 21.906665586437995 24-25 21.99623798403547 26.128717687026942 30.098279412964 21.776764915973583 26-27 21.662532144482228 26.403672273838446 30.04003301242641 21.893762569252914 28-29 21.63231169946302 26.71587094278588 29.991849174662516 21.65996818308858 30-31 21.793277997252236 26.271637437699084 29.993764262555633 21.941320302493047 32-33 21.599089195354047 26.361364665934232 29.980032972828635 22.059513165883086 34-35 22.10154294066056 26.41759483201361 29.82705982033521 21.653802406990618 36-37 22.035203415158378 26.21750810521076 29.82863648123092 21.91865199839994 38-39 21.738117737171184 26.618133162507295 29.829434239678065 21.814314860643456 40-41 22.024518759060534 26.437215434770252 29.801931178973234 21.736334627195976 42-43 21.681599995749778 26.451251291668772 29.540179199948994 22.326969512632456 44-45 21.78249437212463 26.674136851014453 29.429311919513278 22.114056857347638 46-47 22.072736826920547 26.858622066520322 28.921146822954206 22.147494283604924 48-49 22.154106746832525 26.911537018794984 28.767364929987703 22.166991304384787 50-51 22.064743715597146 27.153211136648085 28.65189300541625 22.130152142338513 52-53 22.232908566644962 27.24073508112202 28.373280890814144 22.153075461418872 54-55 22.02973906792936 27.169194242169638 28.42581423561904 22.37525245428197 56-57 22.092323464108528 27.485552049802937 28.30935079835548 22.112773687733053 58-59 22.255847608039957 27.062448228488083 28.476954157961178 22.20475000551079 60-61 22.219306962507694 27.112304833246846 28.722900256159267 21.945487948086186 62-63 22.020795913375416 27.099304456878 28.907227895876485 21.9726717338701 64-65 21.974256381329344 27.072680522159708 28.72149269221801 22.231570404292942 66-67 21.916433779914648 27.32780743507831 28.346949783304442 22.408809001702597 68-69 21.882904886913305 27.238626790003707 28.61565078633207 22.262817536750916 70-71 22.105377219084204 27.13850612382785 28.43272181812194 22.32339483896601 72-73 22.00988361995017 27.21386872440858 28.375305609836026 22.400942045805223 74-75 21.8641639682823 27.599266549382556 28.155436500854137 22.38113298148101 76-77 22.107685181348693 27.339620697092066 28.15850254926146 22.39419157229778 78-79 22.111683416151116 27.322377374968525 28.17114010176122 22.39479910711914 80-81 22.037615623316842 27.58618033784796 28.00846372771592 22.36774031111928 82-83 22.206812922813306 27.45573315791165 28.00202262546665 22.33543129380839 84-85 22.09094049886701 27.50179050708595 27.85731603718094 22.549952956866107 86-87 22.024086300879862 27.45212123400932 28.07585503818803 22.447937426922792 88-89 22.097483312154008 27.367376937509707 27.923722568129033 22.611417182207244 90-91 22.114685383034477 27.238960372556715 28.04262708366643 22.603727160742373 92-93 22.176385247292604 27.12505235002134 28.088133402062237 22.610429000623817 94-95 22.046790723442026 27.083522007033135 28.087660036241285 22.782027233283557 96-97 22.01937609700637 27.078200470021336 27.997554550546415 22.904868882425884 98-99 21.883290498399965 27.12451221426373 27.935512023305886 23.05668526403042 100-101 22.244549318070664 26.355959520219972 28.50742361185972 22.89206754984964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2516.0 1 1455.5 2 422.0 3 480.0 4 445.0 5 416.5 6 713.5 7 1490.0 8 2500.5 9 2997.5 10 2719.0 11 2441.0 12 2579.5 13 2777.0 14 2957.5 15 3100.0 16 3199.0 17 3390.5 18 3586.5 19 3767.5 20 4010.5 21 4539.5 22 5440.0 23 6536.5 24 7774.0 25 9511.5 26 11822.5 27 14352.5 28 16394.5 29 18566.0 30 21562.5 31 24667.5 32 28649.5 33 32492.0 34 36325.5 35 40088.0 36 44606.0 37 49560.5 38 52281.5 39 54010.0 40 55162.0 41 57392.0 42 60200.5 43 62570.5 44 65618.0 45 68256.5 46 69247.0 47 71596.0 48 75682.0 49 75584.0 50 71996.0 51 68830.0 52 66439.0 53 65466.5 54 64022.0 55 58626.0 56 51617.5 57 45571.5 58 40599.5 59 35659.5 60 29793.0 61 24421.5 62 19704.5 63 14960.0 64 10957.0 65 8277.0 66 6014.5 67 4465.0 68 3354.5 69 2341.0 70 1744.0 71 1238.0 72 848.0 73 602.5 74 427.5 75 300.0 76 195.0 77 119.0 78 77.0 79 51.0 80 32.5 81 21.5 82 17.5 83 15.0 84 14.0 85 10.0 86 5.5 87 5.5 88 5.5 89 1.5 90 0.0 91 2.0 92 3.5 93 2.0 94 0.5 95 1.0 96 1.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35375463998095497 2 0.31059572557080845 3 0.30858093595460995 4 0.3113910372614131 5 0.3222602970330102 6 0.2117119720389618 7 0.21144686814209357 8 0.21229520061207188 9 0.20975020320213694 10-11 0.21714660192476035 12-13 0.20617130059441593 14-15 0.20810655904155398 16-17 0.21624524867540837 18-19 0.2077619239756253 20-21 0.19941115122427633 22-23 0.1980591213502484 24-25 0.2024068252588872 26-27 0.19784703823275382 28-29 0.21393884477265485 30-31 0.22048691102529994 32-33 0.22271378375899298 34-35 0.2205399318046736 36-37 0.19204126289133974 38-39 0.18217939792784188 40-41 0.1896023070401521 42-43 0.20137292006110113 44-45 0.20699312267470743 46-47 0.2103069213855602 48-49 0.2096971824227633 50-51 0.21380629282422076 52-53 0.24538016694122594 54-55 0.200498077201436 56-57 0.18202033558972097 58-59 0.17928976545197828 60-61 0.1717873251706076 62-63 0.18151663818567135 64-65 0.17677127843173018 66-67 0.17722195505640614 68-69 0.1863680394983598 70-71 0.18064179532600622 72-73 0.17719544466671933 74-75 0.1826565849422047 76-77 0.18806470443831644 78-79 0.1912459512007351 80-81 0.18191429403097367 82-83 0.17769914207076895 84-85 0.17001112906159052 86-87 0.16958696282660138 88-89 0.176055497910186 90-91 0.18525460313151326 92-93 0.1746239368670976 94-95 0.17992601480446202 96-97 0.1788125784376155 98-99 0.182311949876276 100-101 0.15588109135851433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1886053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.88720797591601 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16452905855692 44.46561820893658 2 12.459142591955805 14.175316716603664 3 4.003665187994041 6.832720026460555 4 1.7506665563014703 3.9836212993920963 5 0.9259784523740769 2.633816440071048 6 0.5782402366652789 1.973668356193237 7 0.36334137166244096 1.4468633323211113 8 0.263932456753687 1.2011504447153158 9 0.2008515066275927 1.0283293286819948 >10 1.155649953240272 12.428908827278406 >50 0.08861039038802479 3.4714493635015984 >100 0.04361809516711798 4.393189668483881 >500 0.0010271350234981693 0.3539315559821342 >1k 4.6687955613553136E-4 0.46255980631733806 >5k 2.801277336813188E-4 1.1488566250610228 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.491184500117441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7317 0.3879530426769555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 0.26759587349878294 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2697 0.14299704197071875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.302077937364433E-5 0.0 14-15 0.0 0.0 0.0 5.302077937364433E-5 0.0 16-17 0.0 0.0 0.0 5.302077937364433E-5 0.0 18-19 0.0 0.0 0.0 5.302077937364433E-5 0.0 20-21 0.0 0.0 0.0 7.95311690604665E-5 0.0 22-23 0.0 0.0 0.0 1.325519484341108E-4 0.0 24-25 0.0 2.6510389686822164E-5 0.0 2.120831174945773E-4 0.0 26-27 0.0 5.302077937364433E-5 0.0 3.446350659286881E-4 0.0 28-29 0.0 5.302077937364433E-5 0.0 6.362493524837318E-4 0.0 30-31 0.0 5.302077937364433E-5 0.0 0.001776196109017085 0.0 32-33 0.0 5.302077937364433E-5 0.0 0.003419840269600059 0.0 34-35 0.0 5.302077937364433E-5 0.0 0.004877911702375278 0.0 36-37 0.0 5.302077937364433E-5 0.0 0.006707128590766007 0.0 38-39 0.0 5.302077937364433E-5 0.0 0.00994139613255831 0.0 40-41 0.0 5.302077937364433E-5 0.0 0.013546809129966125 0.0 42-43 0.0 5.302077937364433E-5 0.0 0.01725826368612123 0.0 44-45 0.0 5.302077937364433E-5 0.0 0.01898143901576467 0.0 46-47 2.6510389686822164E-5 5.302077937364433E-5 0.0 0.022162685778183328 0.0 48-49 5.302077937364433E-5 5.302077937364433E-5 0.0 0.026457368907448518 0.0 50-51 5.302077937364433E-5 5.302077937364433E-5 0.0 0.03231616502823621 0.0 52-53 5.302077937364433E-5 5.302077937364433E-5 0.0 0.04323844557920695 0.0 54-55 7.95311690604665E-5 7.95311690604665E-5 0.0 0.051483176771808636 0.0 56-57 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.0703320638391392 0.0 58-59 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.09875120158341255 0.0 60-61 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.12648106919582852 0.0 62-63 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.14026647183297608 0.0 64-65 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.16404629138205554 0.0 66-67 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.21033343177524705 0.0 68-69 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.2736667527370652 0.0 70-71 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.3350913256414321 0.0 72-73 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.406218701171176 0.0 74-75 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.48991200141247354 0.0 76-77 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.5516016782137088 0.0 78-79 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.6404114836645629 0.0 80-81 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.7599998515418178 0.0 82-83 1.0604155874728865E-4 1.0604155874728865E-4 0.0 0.8713434882264708 0.0 84-85 1.0604155874728865E-4 1.0604155874728865E-4 0.0 1.0049823626377412 0.0 86-87 1.0604155874728865E-4 1.0604155874728865E-4 0.0 1.1614997033487393 0.0 88-89 1.0604155874728865E-4 1.0604155874728865E-4 0.0 1.3814298961906162 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6530 0.0 39.336124 1 GTATCAA 10045 0.0 33.053425 1 ATCAACG 12450 0.0 26.54107 3 TCAACGC 12555 0.0 26.244062 4 TATCAAC 12665 0.0 26.20383 2 GTACATG 8715 0.0 26.089846 1 CAACGCA 12805 0.0 25.731684 5 TACATGG 8725 0.0 25.339735 2 AACGCAG 13100 0.0 25.091238 6 ACATGGG 8740 0.0 24.479586 3 GAGTACT 8200 0.0 22.649662 12-13 CGCAGAG 14745 0.0 22.034853 8 ACGCAGA 14775 0.0 21.990696 7 CATGGGG 4390 0.0 21.227856 4 GTACTTT 8740 0.0 21.114386 14-15 AGAGTAC 12735 0.0 20.551603 10-11 GTACCTG 1480 0.0 20.248358 1 GCAGAGT 15675 0.0 20.24213 9 AGTACTT 8710 0.0 20.096397 12-13 CATGGGA 4420 0.0 19.25508 4 >>END_MODULE