FastQCFastQC Report
Thu 26 May 2016
SRR937727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886053
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105470.5592101600538267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92940.4927751234986504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.3357805957732895No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.12406862373432771No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21610.11457790422644538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81650.035.092921
GTATCAA116900.029.9985471
TATCAAC142850.024.5464322
ATCAACG142650.024.3810043
TCAACGC144150.024.094344
GTACATG90350.024.0351241
CAACGCA145900.023.8379045
GACCGTA1000.001653449123.7514517
TACATGG90850.023.6388172
AACGCAG149900.023.289486
GAGTACT90250.022.6592812-13
ACATGGG90600.022.550313
GTACTTT93850.021.61064114-15
GTACCTG14600.021.1552851
TACCTGG14650.020.7565232
GCACCGT3001.6734703E-1020.5824076
ACGCAGA170200.020.4022457
CGCAGAG169850.020.2764828
AGTACTT94300.019.97338512-13
AGAGTAC140500.019.62664410-11
Produced by FastQC (version 0.11.5)