##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937727_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886053 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12448059519006 33.0 31.0 34.0 31.0 34.0 2 32.41104942437991 34.0 31.0 34.0 31.0 34.0 3 32.49931947829674 34.0 31.0 34.0 31.0 34.0 4 36.14188890768181 37.0 35.0 37.0 35.0 37.0 5 36.05400007316867 37.0 35.0 37.0 35.0 37.0 6 36.09273652437127 37.0 35.0 37.0 35.0 37.0 7 36.03730860161406 37.0 35.0 37.0 35.0 37.0 8 36.00893347111666 37.0 35.0 37.0 35.0 37.0 9 37.703985518964735 39.0 37.0 39.0 35.0 39.0 10-11 37.61874348175793 39.0 37.0 39.0 35.0 39.0 12-13 37.50924974006563 39.0 37.0 39.0 35.0 39.0 14-15 38.71017251370985 40.0 38.0 41.0 34.5 41.0 16-17 38.66638132650567 40.0 38.0 41.0 34.5 41.0 18-19 38.64929538035251 40.0 38.0 41.0 34.0 41.0 20-21 38.55431183535139 40.0 38.0 41.0 34.0 41.0 22-23 38.501690037342534 40.0 38.0 41.0 34.0 41.0 24-25 38.52142331100981 40.0 38.0 41.0 34.0 41.0 26-27 38.384556531550274 40.0 38.0 41.0 34.0 41.0 28-29 38.23659144255225 40.0 38.0 41.0 34.0 41.0 30-31 38.270520764792934 40.0 38.0 41.0 34.0 41.0 32-33 38.19048616343231 40.0 38.0 41.0 34.0 41.0 34-35 38.05292825811364 40.0 37.0 41.0 33.5 41.0 36-37 37.90246562530321 40.0 37.0 41.0 33.0 41.0 38-39 37.772704160487535 40.0 37.0 41.0 33.0 41.0 40-41 37.596754969239996 40.0 37.0 41.0 33.0 41.0 42-43 37.36051717528616 40.0 37.0 41.0 32.5 41.0 44-45 37.04958317714295 40.0 36.0 41.0 31.5 41.0 46-47 36.659752668668375 39.5 36.0 41.0 31.0 41.0 48-49 36.304779876281316 39.0 36.0 41.0 30.0 41.0 50-51 36.03360510017481 39.0 35.0 41.0 29.5 41.0 52-53 35.78902660741771 39.0 35.0 40.0 28.5 41.0 54-55 35.55380575201227 39.0 35.0 40.0 28.0 41.0 56-57 35.272001635160834 38.0 35.0 40.0 27.5 41.0 58-59 35.00560641721097 38.0 34.0 40.0 27.0 41.0 60-61 34.665797567724766 38.0 34.0 40.0 26.0 41.0 62-63 34.27979038765082 37.0 34.0 40.0 26.0 41.0 64-65 33.85328010400556 37.0 33.0 39.5 25.5 41.0 66-67 33.420448152835576 36.0 33.0 39.0 24.5 40.0 68-69 32.960159656170845 35.5 32.0 39.0 23.0 40.0 70-71 32.55389164567485 35.0 32.0 38.0 22.0 40.0 72-73 32.051454545550946 35.0 31.0 37.5 21.0 39.5 74-75 31.56532928820134 35.0 31.0 37.0 20.0 39.0 76-77 30.35824337916273 33.5 29.5 35.5 19.5 38.0 78-79 30.946953240444465 34.0 31.0 36.0 19.5 38.0 80-81 30.755347013047885 34.0 31.0 35.5 18.0 37.0 82-83 30.381948969620687 34.0 30.5 35.0 17.0 37.0 84-85 29.99815540708559 34.0 30.0 35.0 11.5 36.5 86-87 29.62048282842529 34.0 30.0 35.0 7.0 36.0 88-89 29.172320979315003 34.0 29.0 35.0 2.0 36.0 90-91 28.61646199762149 34.0 29.0 35.0 2.0 35.0 92-93 28.148348164129004 33.0 28.5 35.0 2.0 35.0 94-95 27.649717690860225 33.0 27.0 35.0 2.0 35.0 96-97 27.049375070583913 33.0 26.5 35.0 2.0 35.0 98-99 26.12130544581727 32.5 24.5 34.0 2.0 35.0 100-101 23.335268149940646 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35689073240535407 1101 2 0.3099353840981891 1101 3 0.26479922005000844 1101 4 0.1697745954388452 1101 5 0.07250415223866469 1101 6 0.09382599904906641 1101 7 0.1918069821943007 1101 8 0.15973854164580814 1101 9 0.2831852133350665 1101 10-11 0.2990060380810746 1101 12-13 0.28969722781285867 1101 14-15 0.36082781251474927 1101 16-17 0.22410527117013856 1101 18-19 0.27687737360043485 1101 20-21 0.322297142750152 1101 22-23 0.2872034425924852 1101 24-25 0.25541129953334263 1101 26-27 0.20225108591158403 1101 28-29 0.1304330958371196 1101 30-31 0.27880816652259455 1101 32-33 0.3757815910219904 1101 34-35 0.4056806087820988 1101 36-37 0.3673967456532168 1101 38-39 0.36762322181147766 1101 40-41 0.5087495189605065 1101 42-43 0.5976654824050556 1101 44-45 0.48771800235647333 1101 46-47 0.5050412307265049 1101 48-49 0.43520165622353346 1101 50-51 0.4176667068032458 1101 52-53 0.5865002875415186 1101 54-55 0.4638378183257075 1101 56-57 0.4131816320947763 1101 58-59 0.43500049262919305 1101 60-61 0.5850286045296684 1101 62-63 0.4634022999048142 1101 64-65 0.28860837346021384 1101 66-67 0.43448461310979525 1101 68-69 0.5137216345274993 1101 70-71 0.3643510333974973 1101 72-73 0.24540342983087982 1101 74-75 0.168182151322533 1101 76-77 0.1856005490872512 1101 78-79 0.1487971067549161 1101 80-81 0.2756281473080513 1101 82-83 0.2372308745329086 1101 84-85 0.3087520163723454 1101 86-87 0.30890605630690615 1101 88-89 0.3767441403086558 1101 90-91 0.3784476168031823 1101 92-93 0.15876910473339123 1101 94-95 4.725826296336777E-5 1101 96-97 -0.21667293905023755 1101 98-99 -0.08523026554749791 1101 100-101 0.013783072582594258 1102 1 0.26709773251261026 1102 2 0.14357665541491116 1102 3 0.13958691683119895 1102 4 0.09609542772943058 1102 5 0.03575013319512976 1102 6 0.05586120217611068 1102 7 0.0916015313106513 1102 8 0.11220907660463553 1102 9 0.17557447364038126 1102 10-11 0.13989719916457943 1102 12-13 0.2524604468611571 1102 14-15 0.37173470417635457 1102 16-17 0.28457575846055505 1102 18-19 0.1506037330441501 1102 20-21 0.27988047310420683 1102 22-23 0.29023729908670504 1102 24-25 0.19961112554361904 1102 26-27 0.30064239595889575 1102 28-29 0.2394291989439168 1102 30-31 0.29972626026253124 1102 32-33 0.3813610602341484 1102 34-35 0.27328372152982894 1102 36-37 0.0989761195285439 1102 38-39 0.169468585461928 1102 40-41 0.2604074265552825 1102 42-43 0.23868677827100981 1102 44-45 0.24553323956644135 1102 46-47 0.2612642235881637 1102 48-49 0.18594790531485472 1102 50-51 0.179671759655605 1102 52-53 0.27985459497898546 1102 54-55 0.31713158687170306 1102 56-57 0.38817203882555873 1102 58-59 0.23812003310563767 1102 60-61 0.18934652904673044 1102 62-63 0.21438415015447276 1102 64-65 0.28767145069497246 1102 66-67 0.18766457330666242 1102 68-69 0.32325436485534453 1102 70-71 0.21549626715150083 1102 72-73 0.31256620556288084 1102 74-75 0.10915530208582425 1102 76-77 0.17280801396158907 1102 78-79 0.41536319305858527 1102 80-81 0.4189088070389637 1102 82-83 0.4634558933023456 1102 84-85 0.38801982946447566 1102 86-87 0.34337866484193214 1102 88-89 0.33170570764625396 1102 90-91 0.4074507510016936 1102 92-93 0.30584631565422526 1102 94-95 0.11436875791141077 1102 96-97 0.28088858300236197 1102 98-99 -0.025540348609712993 1102 100-101 0.05905007531161388 1103 1 0.24749124902470498 1103 2 0.14196791830821098 1103 3 0.1399417173281492 1103 4 0.08445982055251733 1103 5 0.06893264742470961 1103 6 0.06523047783991842 1103 7 0.13512757794322283 1103 8 0.08431852157877984 1103 9 0.060087489733525956 1103 10-11 0.13102718674078062 1103 12-13 0.13970149739422055 1103 14-15 0.1533919807647166 1103 16-17 0.14765628199352676 1103 18-19 0.14226245425994222 1103 20-21 0.031075757840277163 1103 22-23 0.04188966394181648 1103 24-25 -0.10956745929934897 1103 26-27 0.030473374549508492 1103 28-29 0.01202230518180869 1103 30-31 0.08500261749000515 1103 32-33 -0.02420595603235398 1103 34-35 0.023136080606413145 1103 36-37 0.04652632033175763 1103 38-39 0.023532322424166807 1103 40-41 0.04461649303703297 1103 42-43 0.07666198846430206 1103 44-45 0.174853397897607 1103 46-47 0.07923018816589433 1103 48-49 -0.05352913955528038 1103 50-51 -0.07865825103463919 1103 52-53 0.01694453748280722 1103 54-55 -0.10171733399160132 1103 56-57 -0.1468960031264217 1103 58-59 0.0019685205807178363 1103 60-61 -0.024324269254393016 1103 62-63 -0.2205839235793121 1103 64-65 0.02297352229070526 1103 66-67 -0.09840575115666184 1103 68-69 -0.045170124468860706 1103 70-71 -0.10063733199327629 1103 72-73 -0.19679960299509247 1103 74-75 -0.08800340468370038 1103 76-77 -0.07983363175798175 1103 78-79 -0.06693203100313028 1103 80-81 0.004444619352963031 1103 82-83 -0.12590413674590195 1103 84-85 -0.07151326694995674 1103 86-87 -0.1354228722416515 1103 88-89 -0.106579230668153 1103 90-91 -0.0751206858247464 1103 92-93 -0.0580114174364077 1103 94-95 0.046883854268056524 1103 96-97 -0.018815557299852514 1103 98-99 -0.04749926340559085 1103 100-101 -8.811604226899306E-4 1104 1 0.32307333338048494 1104 2 0.20471150659253823 1104 3 0.17433527274905458 1104 4 0.08458971198604814 1104 5 0.03248024582806863 1104 6 0.048489659233752036 1104 7 0.07101678188951155 1104 8 0.10452021039059645 1104 9 0.14670119551157512 1104 10-11 0.17830789172542438 1104 12-13 0.17300124196338373 1104 14-15 0.12463843636896144 1104 16-17 0.13281652048491566 1104 18-19 0.20346665051206259 1104 20-21 0.1192904536499313 1104 22-23 0.13423187207386178 1104 24-25 0.004024161587281583 1104 26-27 0.21649263731519142 1104 28-29 0.08546876904376433 1104 30-31 0.07791561143174874 1104 32-33 0.17336036503829177 1104 34-35 0.14781701524447044 1104 36-37 0.13366762636556473 1104 38-39 0.08307928699303346 1104 40-41 0.055968937372867344 1104 42-43 0.2014227103660744 1104 44-45 0.05511012318280706 1104 46-47 -0.21937939322436506 1104 48-49 -0.052577606691293965 1104 50-51 0.03650110589117617 1104 52-53 0.11841239995411712 1104 54-55 0.026242801390090165 1104 56-57 0.13923540817078361 1104 58-59 0.2194687130744697 1104 60-61 0.2829124913603849 1104 62-63 0.24471341509698163 1104 64-65 0.14070805499958539 1104 66-67 0.028018674681916877 1104 68-69 0.14353289171289418 1104 70-71 -0.033468507722766105 1104 72-73 0.17547683364991684 1104 74-75 0.10140207419053127 1104 76-77 0.07280431372555896 1104 78-79 0.044244992943095696 1104 80-81 0.023716647054211393 1104 82-83 0.023467106138802762 1104 84-85 -0.02581737136303275 1104 86-87 0.0044633946406058556 1104 88-89 0.08591775359645837 1104 90-91 0.02485666128778874 1104 92-93 0.19446382898348702 1104 94-95 0.04362674845898695 1104 96-97 0.2906441992318243 1104 98-99 0.543792319428654 1104 100-101 0.10778016551888214 1105 1 0.1603959252836944 1105 2 0.13287511792165674 1105 3 0.11151058163968486 1105 4 0.0030004358214981153 1105 5 0.026132708271553895 1105 6 0.045549207930477564 1105 7 -0.006165506991813174 1105 8 -0.0015723925555874985 1105 9 0.01855325443520428 1105 10-11 0.12674028044492758 1105 12-13 0.04912246808036258 1105 14-15 0.1487227683734389 1105 16-17 0.035092527027352105 1105 18-19 0.09698815131424254 1105 20-21 -0.06269370420203302 1105 22-23 -0.0028379122062247575 1105 24-25 0.02552170817263999 1105 26-27 -0.013227299239808588 1105 28-29 -0.028779419110392723 1105 30-31 -0.026449899600798688 1105 32-33 0.056878840309678935 1105 34-35 0.16875991735791018 1105 36-37 0.08987209147603181 1105 38-39 0.23429505552126528 1105 40-41 0.2486880865085297 1105 42-43 0.17867295431848618 1105 44-45 0.1528161023609016 1105 46-47 0.09480776341911934 1105 48-49 0.05479748272897922 1105 50-51 0.19127870984442552 1105 52-53 0.17765984583314776 1105 54-55 0.03437972120006094 1105 56-57 0.07886402318088415 1105 58-59 0.2649886268837349 1105 60-61 0.20162500863672506 1105 62-63 0.16267323934706468 1105 64-65 0.1621459157192504 1105 66-67 0.16056535554007922 1105 68-69 0.25544237060970687 1105 70-71 0.04445800964386848 1105 72-73 0.09972049435986463 1105 74-75 0.06910225407287385 1105 76-77 0.004301000834448843 1105 78-79 0.08129591648962631 1105 80-81 -0.06427171355086259 1105 82-83 -0.00360477474296772 1105 84-85 0.07784124198020947 1105 86-87 0.1872787053022833 1105 88-89 0.14197330789800944 1105 90-91 0.3089834114944132 1105 92-93 0.44412090655514547 1105 94-95 0.35939134827285457 1105 96-97 0.4637973449156867 1105 98-99 0.2438803940624652 1105 100-101 0.02276242470920309 1106 1 -0.009308086457991749 1106 2 0.026941661427223096 1106 3 0.023836793048396032 1106 4 0.012439237410426074 1106 5 -0.01487373212918186 1106 6 -0.0676506927617595 1106 7 -0.1721609943300777 1106 8 -0.16637673434955502 1106 9 -0.06843231673049388 1106 10-11 -0.14570391370175884 1106 12-13 -0.3681034132760672 1106 14-15 -0.21788946765775563 1106 16-17 -0.11054212992272738 1106 18-19 -0.05562725745404862 1106 20-21 -0.06987526216563822 1106 22-23 -0.06558709437755539 1106 24-25 -0.15184397077118916 1106 26-27 -0.033708156937628075 1106 28-29 -0.20484944203880673 1106 30-31 -0.0069438644633734725 1106 32-33 -0.11551643015795321 1106 34-35 -0.41557181804530074 1106 36-37 -0.16738431241449092 1106 38-39 -0.21029705243211794 1106 40-41 -0.37481043117701773 1106 42-43 -0.534284244764379 1106 44-45 -0.44385562705463855 1106 46-47 -0.43197110520254256 1106 48-49 -0.22089657632992754 1106 50-51 -0.006939774543809563 1106 52-53 0.10302932914067497 1106 54-55 -0.10323044835334372 1106 56-57 0.005594880878703634 1106 58-59 0.010314407471987863 1106 60-61 -0.0795569911003966 1106 62-63 -0.1658917086624001 1106 64-65 -0.5698016118094458 1106 66-67 -0.41753819471257714 1106 68-69 -0.09589353791031385 1106 70-71 -0.33994428173680546 1106 72-73 -0.28898178679795095 1106 74-75 -0.20914934507424476 1106 76-77 -0.1320847076096321 1106 78-79 -0.35953153261514714 1106 80-81 -0.6194281415381262 1106 82-83 -0.788609393284311 1106 84-85 -0.7368616927103275 1106 86-87 -0.36364984056914196 1106 88-89 -0.43959825576119016 1106 90-91 -0.6179893263330847 1106 92-93 -0.7565830102599982 1106 94-95 -0.6122023405725479 1106 96-97 -0.5354090144323855 1106 98-99 -0.6444339661326417 1106 100-101 -0.9168474089502858 1107 1 0.14698607751966364 1107 2 0.05154221890579436 1107 3 -0.025923383169946135 1107 4 -0.04857757446011135 1107 5 0.003305301537388061 1107 6 -0.01785700079753383 1107 7 -0.02941184665411356 1107 8 -0.008680495002437283 1107 9 -0.10070239405877146 1107 10-11 0.029652796243659907 1107 12-13 0.014579140474815233 1107 14-15 -0.05762322229642791 1107 16-17 -0.06846334371408602 1107 18-19 0.07100942813660538 1107 20-21 -0.046854134211834264 1107 22-23 -0.11013045164982316 1107 24-25 -0.05218280565214428 1107 26-27 -0.10937972869577095 1107 28-29 -0.06244383075814852 1107 30-31 -0.12814564601146117 1107 32-33 -0.06233043266418292 1107 34-35 -0.11979722260829107 1107 36-37 -0.05640739359149194 1107 38-39 -0.018946349253148753 1107 40-41 -0.09532285900179005 1107 42-43 -0.14581300965328836 1107 44-45 -0.07427749719602872 1107 46-47 -0.267951096302248 1107 48-49 -0.2643308882609787 1107 50-51 -0.11729246259973536 1107 52-53 -0.24574110848185882 1107 54-55 -0.32476184795226715 1107 56-57 -0.3234671344230975 1107 58-59 -0.27682522843529256 1107 60-61 -0.13270854185533665 1107 62-63 -0.11328129755453631 1107 64-65 -0.10707973291673767 1107 66-67 -0.3021465073623446 1107 68-69 -0.17255708264732306 1107 70-71 -0.3119176702345712 1107 72-73 -0.24500982644335423 1107 74-75 -0.38501926487851534 1107 76-77 -0.3208628445437931 1107 78-79 -0.2571777031618794 1107 80-81 -0.3344100057956325 1107 82-83 -0.2244376790842466 1107 84-85 -0.22962601557123463 1107 86-87 -0.4059616077613235 1107 88-89 -0.42211211359112255 1107 90-91 -0.38152922217359375 1107 92-93 -0.2840857054845607 1107 94-95 -0.3270233757994596 1107 96-97 -0.5098509307137782 1107 98-99 -0.4153917809804959 1107 100-101 -0.24265444018137927 1108 1 0.1263907743493391 1108 2 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-0.5864829083122487 2316 84-85 -0.529303373072306 2316 86-87 -0.4799395284653052 2316 88-89 -0.5645627993122879 2316 90-91 -0.4812303816662933 2316 92-93 -0.4569652867630687 2316 94-95 -0.27384331479135327 2316 96-97 -0.15373744814064239 2316 98-99 -0.19466066969060591 2316 100-101 -0.042270553676047484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 17.0 5 34.0 6 68.0 7 177.0 8 468.0 9 867.0 10 1473.0 11 2443.0 12 3725.0 13 5542.0 14 7327.0 15 10013.0 16 14067.0 17 20328.0 18 18629.0 19 11343.0 20 9431.0 21 9628.0 22 10015.0 23 10771.0 24 12625.0 25 15029.0 26 18366.0 27 22842.0 28 28526.0 29 35818.0 30 45160.0 31 56202.0 32 72263.0 33 95741.0 34 131654.0 35 187815.0 36 284081.0 37 417881.0 38 301469.0 39 24210.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1803357624442 19.602192881513762 15.283671432616833 21.933799923425205 2 15.798548609185426 20.64920763096265 40.09150326104304 23.46074049880889 3 19.693879228208328 24.561080733150128 30.03526412036141 25.70977591828013 4 13.116757588466497 16.90228217340658 36.44531728429689 33.535642953830035 5 13.457389900327222 38.03393574073514 33.863019017445254 14.64565534149238 6 32.31308982303254 36.235142914859765 16.820418090053675 14.63134917205402 7 28.71944743864568 33.368680519582426 19.897956208017483 18.013915833754407 8 26.775175459014143 35.59656064808359 18.938916350707004 18.68934754219526 9 26.61971853389062 16.888496770769432 20.227904518059674 36.26388017728028 10-11 25.363232104293992 27.165593967931972 26.301063649855017 21.17011027791902 12-13 26.105740476018784 24.37159857813673 27.585107127556235 21.93755381828825 14-15 22.913963711518182 25.565347315266322 26.315326239506525 25.20536273370897 16-17 21.879395753989947 29.076780981234357 26.920982602291666 22.122840662484034 18-19 21.77091523939147 27.262250848730122 29.832406618477847 21.134427293400556 20-21 22.480810454425193 26.076388097259194 30.34760953165155 21.09519191666406 22-23 22.3790203924517 25.9092988964715 30.252603204278167 21.459077506798632 24-25 22.23158040962881 26.14089029500294 30.320845322682498 21.306683972685757 26-27 21.768856988867896 26.52548486067173 30.30736761289512 21.398290537565252 28-29 21.68326099906391 26.762115559330667 30.237182704143162 21.31744073746226 30-31 21.816831234329044 26.34841120583568 30.300845204244 21.53391235559128 32-33 21.767389357563122 26.35320958636899 30.234092043012577 21.645309013055307 34-35 22.219577074451248 26.446420116507863 30.09809374391918 21.23590906512171 36-37 22.183522944477172 26.246955944504215 30.041785676224368 21.527735434794252 38-39 21.859115305879527 26.63787815082609 30.111189876424472 21.39181666686991 40-41 22.106695008243467 26.42624929117338 30.02617389508544 21.44088180549771 42-43 21.87217879453047 26.513558092075662 29.77188552835768 21.84237758503619 44-45 22.06539264803269 26.68710794447452 29.61783152435271 21.62966788314008 46-47 22.26827666030594 27.02588421428242 29.013262087544728 21.69257703786691 48-49 22.39632184249329 27.10819897426 28.772097072563707 21.723382110682998 50-51 22.33328013581803 27.33568462816262 28.608554478585702 21.722480757433647 52-53 22.56927562481012 27.414738610208726 28.286029077655822 21.72995668732533 54-55 22.32076723188585 27.412034550460675 28.346022089517103 21.921176128136377 56-57 22.335056331927046 27.72329303577365 28.22911127099822 21.712539361301086 58-59 22.571237393646946 27.255199085073436 28.36797269217779 21.805590829101835 60-61 22.447274811471363 27.314980013817213 28.7308734166007 21.50687175811072 62-63 22.26194067717079 27.315563242390322 28.863955572828548 21.55854050761034 64-65 22.204227558822577 27.344220973641782 28.622339881222853 21.82921158631279 66-67 22.301679751311337 27.48960395068431 28.25278504898855 21.9559312490158 68-69 22.22005426146561 27.405592525766775 28.53435189785229 21.840001314915327 70-71 22.3739735839873 27.368690063322713 28.353206405122233 21.904129947567753 72-73 22.394969812619266 27.380354634784922 28.255833743802533 21.968841808793286 74-75 22.15242625737453 27.855155714075902 27.89484176743708 22.097576261112494 76-77 22.331291856591516 27.510043461132856 28.15018984090055 22.008474841375083 78-79 22.45125136992439 27.52382886377 28.018698308053906 22.006221458251705 80-81 22.313370833163226 27.876708660891293 27.906294255781784 21.9036262501637 82-83 22.44637506266438 27.803898062580345 27.825186057874184 21.924540816881088 84-85 22.308466411071162 27.754787378721595 27.7805289671075 22.156217243099743 86-87 22.325459570860414 27.78158938269497 27.942640000042417 21.950311046402195 88-89 22.342558772208417 27.687928175931432 27.828804386727203 22.14070866513295 90-91 22.393828228900865 27.57056376775628 27.896868828918258 22.1387391744246 92-93 22.431325494766348 27.5414610635697 27.90280466793371 22.124408773730238 94-95 22.35843849563082 27.51057366892659 27.8356440672664 22.295343768176185 96-97 22.25157511480324 27.52799099495083 27.811705185379203 22.408728704866725 98-99 22.114668060072326 27.581929522199182 27.641949887766685 22.6614525299618 100-101 22.232451288491134 27.436344390977542 27.95920228838825 22.372002032143072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 508.0 1 377.5 2 331.5 3 435.0 4 384.0 5 347.0 6 668.0 7 1636.5 8 2653.0 9 3154.5 10 3001.5 11 2675.5 12 2797.5 13 3041.0 14 3289.0 15 3469.0 16 3560.0 17 3697.5 18 3900.0 19 4139.0 20 4425.0 21 4886.5 22 5667.0 23 6730.5 24 7944.5 25 9460.5 26 11687.0 27 14353.0 28 16440.0 29 18865.5 30 21797.0 31 24632.5 32 28441.0 33 32329.0 34 36289.5 35 39876.5 36 44518.5 37 49566.5 38 52412.5 39 53906.5 40 54946.5 41 57983.0 42 61012.0 43 63275.5 44 66503.0 45 68838.0 46 69447.5 47 72152.5 48 76872.0 49 76155.0 50 72245.5 51 69395.0 52 66690.5 53 65296.5 54 64509.0 55 59204.5 56 51097.5 57 44633.0 58 39966.0 59 35236.0 60 29018.0 61 23453.5 62 18720.5 63 14142.5 64 10265.0 65 7590.0 66 5463.5 67 4249.5 68 3114.0 69 2081.0 70 1522.5 71 940.5 72 618.0 73 472.0 74 333.0 75 216.5 76 140.0 77 83.0 78 49.0 79 28.5 80 20.5 81 10.5 82 5.5 83 6.0 84 3.0 85 2.5 86 2.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01670154550269796 2 0.0 3 0.0 4 0.0 5 0.04156829102893715 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004082600011770613 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.362493524837318E-4 24-25 2.120831174945773E-4 26-27 0.0011399467565333529 28-29 9.278636390387756E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.157805215441984E-4 42-43 0.011850144190009506 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.157805215441984E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012778007829048282 92-93 0.0019352584471380178 94-95 0.0 96-97 0.0 98-99 0.0014050506534015747 100-101 0.019034459795138313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1886053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.18630894027795 #Duplication Level Percentage of deduplicated Percentage of total 1 76.15359014060094 41.26481962269907 2 13.437833966355553 14.562932455782054 3 4.410316605185893 7.16936334279122 4 1.9567871129357584 4.241242841275662 5 1.0313241611253643 2.7941824806155995 6 0.6421165437448128 2.087635524901196 7 0.42650812704300367 1.6177630796244058 8 0.2941275623364853 1.2750149568492524 9 0.21704287897442112 1.0584677244055807 >10 1.2773183601265135 12.996319872771087 >50 0.10205944467033302 3.805538096388111 >100 0.0484268493270434 4.739686303347536 >500 0.00196019044140746 0.6698479551427312 >1k 2.94028566211119E-4 0.32714369330684406 >5k 1.9601904414074598E-4 0.8299146907964371 >10k+ 9.800952207037299E-5 0.5601273593031306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10547 0.5592101600538267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9294 0.4927751234986504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6333 0.3357805957732895 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2340 0.12406862373432771 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2161 0.11457790422644538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.302077937364433E-5 0.0 14-15 0.0 0.0 0.0 5.302077937364433E-5 2.6510389686822164E-5 16-17 0.0 0.0 0.0 5.302077937364433E-5 5.302077937364433E-5 18-19 0.0 0.0 0.0 5.302077937364433E-5 5.302077937364433E-5 20-21 0.0 7.95311690604665E-5 0.0 7.95311690604665E-5 5.302077937364433E-5 22-23 0.0 1.0604155874728865E-4 0.0 1.325519484341108E-4 5.302077937364433E-5 24-25 0.0 1.0604155874728865E-4 0.0 2.120831174945773E-4 5.302077937364433E-5 26-27 0.0 1.0604155874728865E-4 0.0 3.446350659286881E-4 5.302077937364433E-5 28-29 0.0 1.0604155874728865E-4 0.0 6.362493524837318E-4 5.302077937364433E-5 30-31 0.0 1.0604155874728865E-4 0.0 0.001802706498703907 5.302077937364433E-5 32-33 0.0 1.0604155874728865E-4 0.0 0.0035258818283473476 5.302077937364433E-5 34-35 0.0 1.0604155874728865E-4 0.0 0.004957442871435744 5.302077937364433E-5 36-37 0.0 1.0604155874728865E-4 0.0 0.006866190928886941 5.302077937364433E-5 38-39 0.0 1.0604155874728865E-4 0.0 0.010153479250052888 5.302077937364433E-5 40-41 0.0 1.0604155874728865E-4 0.0 0.013864933806207992 5.302077937364433E-5 42-43 0.0 1.59062338120933E-4 0.0 0.017947533817978603 5.302077937364433E-5 44-45 0.0 2.120831174945773E-4 0.0 0.01975024031668251 5.302077937364433E-5 46-47 0.0 2.120831174945773E-4 0.0 0.023011018248161638 5.302077937364433E-5 48-49 0.0 2.120831174945773E-4 0.0 0.027385232546487295 5.302077937364433E-5 50-51 0.0 2.120831174945773E-4 0.0 0.03332355983633546 5.302077937364433E-5 52-53 0.0 2.120831174945773E-4 0.0 0.044537454673861235 5.302077937364433E-5 54-55 0.0 2.120831174945773E-4 0.0 0.05275567547677611 5.302077937364433E-5 56-57 0.0 2.120831174945773E-4 0.0 0.07168409371316713 5.302077937364433E-5 58-59 0.0 2.120831174945773E-4 0.0 0.10023578340587459 5.302077937364433E-5 60-61 0.0 2.120831174945773E-4 0.0 0.12804518218735103 5.302077937364433E-5 62-63 0.0 2.120831174945773E-4 0.0 0.14177756404512493 5.302077937364433E-5 64-65 0.0 2.120831174945773E-4 0.0 0.1655573835942044 5.302077937364433E-5 66-67 0.0 2.120831174945773E-4 0.0 0.21226869022238506 5.302077937364433E-5 68-69 0.0 2.120831174945773E-4 0.0 0.2758140943016978 5.302077937364433E-5 70-71 0.0 2.120831174945773E-4 0.0 0.3374507503235593 5.302077937364433E-5 72-73 0.0 2.120831174945773E-4 0.0 0.40979760377889696 5.302077937364433E-5 74-75 0.0 2.120831174945773E-4 0.0 0.4943657468798597 5.302077937364433E-5 76-77 0.0 2.120831174945773E-4 0.0 0.5569037561510732 5.302077937364433E-5 78-79 0.0 2.120831174945773E-4 0.0 0.6465353836822189 5.302077937364433E-5 80-81 0.0 2.120831174945773E-4 0.0 0.7688543216972163 5.302077937364433E-5 82-83 0.0 2.120831174945773E-4 0.0 0.8814439466971501 5.302077937364433E-5 84-85 0.0 2.120831174945773E-4 0.0 1.017283183452427 5.302077937364433E-5 86-87 0.0 2.120831174945773E-4 0.0 1.1766106254702282 5.302077937364433E-5 88-89 0.0 2.120831174945773E-4 0.0 1.40083550144137 5.302077937364433E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8165 0.0 35.09292 1 GTATCAA 11690 0.0 29.998547 1 TATCAAC 14285 0.0 24.546432 2 ATCAACG 14265 0.0 24.381004 3 TCAACGC 14415 0.0 24.09434 4 GTACATG 9035 0.0 24.035124 1 CAACGCA 14590 0.0 23.837904 5 GACCGTA 100 0.0016534491 23.751451 7 TACATGG 9085 0.0 23.638817 2 AACGCAG 14990 0.0 23.28948 6 GAGTACT 9025 0.0 22.65928 12-13 ACATGGG 9060 0.0 22.55031 3 GTACTTT 9385 0.0 21.610641 14-15 GTACCTG 1460 0.0 21.155285 1 TACCTGG 1465 0.0 20.756523 2 GCACCGT 300 1.6734703E-10 20.582407 6 ACGCAGA 17020 0.0 20.402245 7 CGCAGAG 16985 0.0 20.276482 8 AGTACTT 9430 0.0 19.973385 12-13 AGAGTAC 14050 0.0 19.626644 10-11 >>END_MODULE