FastQCFastQC Report
Thu 26 May 2016
SRR937726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787507
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89500.500697339926501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69840.39071175665326063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.26288008942062885No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25180.14086658122177984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.037.229971
GTATCAA93700.034.5649571
TATCAAC115550.028.0202182
ATCAACG115150.027.9516163
TCAACGC115250.027.9297144
CAACGCA117050.027.4580465
AACGCAG120600.026.7098926
GTACATG85000.025.4578061
TACATGG85900.024.7405132
ACATGGG87000.023.4433783
GAGTACT73350.022.98934712-13
ACGCAGA138800.022.933757
CGCAGAG139150.022.7053498
GTACCTG14600.021.8248081
CATGGGG41750.021.4101724
GTACTTT77650.021.37952614-15
GTATAGG5350.021.334671
GCAGAGT147350.020.796359
TACCTGG14650.020.770012
AGTACTT77950.020.32254612-13
Produced by FastQC (version 0.11.5)