##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937726_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787507 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.443206655973935 31.0 31.0 34.0 30.0 34.0 2 31.567882531369108 33.0 31.0 34.0 30.0 34.0 3 31.60791314383664 33.0 31.0 34.0 30.0 34.0 4 35.217676350358346 37.0 35.0 37.0 33.0 37.0 5 35.18988233332792 37.0 35.0 37.0 33.0 37.0 6 35.262861068516095 37.0 35.0 37.0 33.0 37.0 7 35.18558025227314 37.0 35.0 37.0 32.0 37.0 8 35.17195792799693 37.0 35.0 37.0 32.0 37.0 9 36.738919064372894 39.0 37.0 39.0 33.0 39.0 10-11 36.61340682861662 39.0 37.0 39.0 32.0 39.0 12-13 36.46650278852055 39.0 37.0 39.0 32.0 39.0 14-15 37.53844124806224 40.0 37.0 41.0 32.0 41.0 16-17 37.46508013674911 40.0 37.0 41.0 32.0 41.0 18-19 37.41796395762366 40.0 37.0 41.0 32.0 41.0 20-21 37.23967318729381 40.0 37.0 41.0 31.5 41.0 22-23 37.08432050895465 39.0 36.5 41.0 31.0 41.0 24-25 36.916625501326706 39.0 36.0 41.0 31.0 41.0 26-27 37.011744289672706 39.0 36.0 41.0 31.0 41.0 28-29 36.95136438626534 39.0 36.0 41.0 31.0 41.0 30-31 36.734367194086516 39.0 36.0 41.0 30.0 41.0 32-33 36.47968455508146 39.0 35.5 40.5 30.0 41.0 34-35 36.19913852085614 39.0 35.0 40.0 30.0 41.0 36-37 36.202515011130025 39.0 35.0 40.0 30.0 41.0 38-39 36.14364615075633 39.0 35.0 41.0 30.0 41.0 40-41 35.941177293291716 39.0 35.0 40.5 28.5 41.0 42-43 35.67128911942723 39.0 35.0 40.0 27.5 41.0 44-45 35.41219419000877 38.5 35.0 40.0 26.5 41.0 46-47 35.0158751825867 38.0 34.5 40.0 24.5 41.0 48-49 34.6456531359038 38.0 34.0 40.0 24.0 41.0 50-51 33.740067871062884 37.5 33.0 39.5 22.5 40.5 52-53 33.837760355623786 38.0 33.0 39.5 23.0 40.5 54-55 34.12103505049211 38.0 34.0 40.0 22.5 41.0 56-57 33.829827239837385 38.0 33.0 40.0 20.5 41.0 58-59 33.574711875254195 38.0 33.0 40.0 19.5 41.0 60-61 33.235950404669744 37.0 32.5 40.0 18.0 41.0 62-63 32.84124425806444 37.0 32.0 40.0 16.0 41.0 64-65 32.44726258414652 36.0 31.5 39.0 13.0 41.0 66-67 32.003103204630804 36.0 31.0 39.0 8.0 40.5 68-69 31.48897766554201 35.0 31.0 38.5 7.5 40.0 70-71 31.02164746767425 35.0 30.0 38.0 4.5 40.0 72-73 30.53369021771663 35.0 29.5 37.0 2.0 39.5 74-75 30.021548726802187 34.0 29.0 37.0 2.0 39.0 76-77 29.53254728512951 34.0 29.0 36.0 2.0 39.0 78-79 28.970429766149167 34.0 28.5 36.0 2.0 38.0 80-81 28.422493170656114 34.0 27.5 35.0 2.0 37.0 82-83 27.880452496130083 33.5 26.0 35.0 2.0 37.0 84-85 27.376775307733062 33.0 26.0 35.0 2.0 36.0 86-87 26.82065496806446 33.0 25.0 35.0 2.0 36.0 88-89 26.29590233772511 32.0 24.5 35.0 2.0 35.0 90-91 25.734853625748038 32.0 22.0 34.5 2.0 35.0 92-93 25.121736026768005 32.0 19.5 34.0 2.0 35.0 94-95 24.4244232330279 31.0 14.0 34.0 2.0 35.0 96-97 23.576514665397113 31.0 2.0 34.0 2.0 35.0 98-99 22.576229631548294 31.0 2.0 34.0 2.0 35.0 100-101 20.0658173646313 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19181652199800325 1101 2 0.2637005304362283 1101 3 0.3162292645618088 1101 4 0.14663169064036907 1101 5 0.21514328016062478 1101 6 0.2563040580261813 1101 7 0.2852965155323872 1101 8 0.31896096833891363 1101 9 0.40459863296746335 1101 10-11 0.21919783426289285 1101 12-13 0.3652953693039649 1101 14-15 0.5176345981463228 1101 16-17 0.6396512915213677 1101 18-19 0.5364298210980891 1101 20-21 0.6316564357974528 1101 22-23 0.5477481098191888 1101 24-25 0.5722999754733991 1101 26-27 0.6605315022225966 1101 28-29 0.5328001852445468 1101 30-31 0.4871821225701183 1101 32-33 0.5349467258150398 1101 34-35 0.508641832921775 1101 36-37 0.5816641049420994 1101 38-39 0.5989340086927157 1101 40-41 0.6383652702956226 1101 42-43 0.571429106826443 1101 44-45 0.5582038843638273 1101 46-47 0.6548640346594823 1101 48-49 0.8030871417858378 1101 50-51 0.6764431933377395 1101 52-53 0.5741829648502659 1101 54-55 0.7870406839194573 1101 56-57 0.9552828644682236 1101 58-59 0.8626462649018976 1101 60-61 0.9337923987519829 1101 62-63 0.8736087755031505 1101 64-65 0.9140260882664677 1101 66-67 1.0545156488068024 1101 68-69 1.2038573225473925 1101 70-71 1.2555009264408348 1101 72-73 1.1988451804511584 1101 74-75 1.0964768953372186 1101 76-77 1.1688985357462123 1101 78-79 1.2731886897051439 1101 80-81 1.3597143789947772 1101 82-83 1.6728253963705377 1101 84-85 1.6182907078867501 1101 86-87 1.735364039009422 1101 88-89 1.8178765481422623 1101 90-91 1.860285697496284 1101 92-93 2.0475212340705724 1101 94-95 2.1457552748948956 1101 96-97 2.138932450643857 1101 98-99 2.347674349491289 1101 100-101 2.3551630364072196 1102 1 0.19379416920640224 1102 2 0.21351112778522818 1102 3 0.18681178592518677 1102 4 0.11591187564850713 1102 5 0.010598324187100161 1102 6 0.2094427355831172 1102 7 0.13449926470732265 1102 8 -0.0016202674594296695 1102 9 0.11775982324500944 1102 10-11 0.08762815463278173 1102 12-13 0.17895790635831332 1102 14-15 0.322552580276259 1102 16-17 0.33105836250649645 1102 18-19 0.25587754235739624 1102 20-21 0.14032633359622082 1102 22-23 0.2338750467470092 1102 24-25 0.42315898059702306 1102 26-27 0.4120237601725236 1102 28-29 0.3935684200410847 1102 30-31 0.2350609992268744 1102 32-33 0.18855346698786235 1102 34-35 0.27254958266164664 1102 36-37 0.36473243496480734 1102 38-39 0.4423238809405703 1102 40-41 0.4158928150008592 1102 42-43 0.4822971085465042 1102 44-45 0.5048828924548658 1102 46-47 0.29136155634956395 1102 48-49 0.42873567396542 1102 50-51 0.32646084921721297 1102 52-53 0.47715063866868235 1102 54-55 0.4681374053511931 1102 56-57 0.5653348980080253 1102 58-59 0.5510010034828312 1102 60-61 0.5227319161013355 1102 62-63 0.5725588108974691 1102 64-65 0.5796566366885187 1102 66-67 0.6778034589847586 1102 68-69 0.7780328920468023 1102 70-71 0.8013131570471437 1102 72-73 0.6360439130019699 1102 74-75 0.51499389109674 1102 76-77 0.681542126874259 1102 78-79 0.6357278198281513 1102 80-81 0.85268269205746 1102 82-83 0.8831531541257398 1102 84-85 1.0116883524957139 1102 86-87 0.8935364231381406 1102 88-89 0.9999887480129495 1102 90-91 1.186760128551196 1102 92-93 1.2069368421510909 1102 94-95 1.190685890944735 1102 96-97 1.4237638918377442 1102 98-99 1.895278115229729 1102 100-101 2.1140234690977486 1103 1 0.3244079100023036 1103 2 0.3831489264740746 1103 3 0.2969598791680994 1103 4 0.2723092138385681 1103 5 0.3951757209340485 1103 6 0.36351444239809183 1103 7 0.35944579942054844 1103 8 0.33969494430060365 1103 9 0.19030068297870883 1103 10-11 0.38206092875068975 1103 12-13 0.39717583987525273 1103 14-15 0.492312810699417 1103 16-17 0.46897673952124563 1103 18-19 0.43567123059567336 1103 20-21 0.440809018708201 1103 22-23 0.3934501228924603 1103 24-25 0.3906602712172642 1103 26-27 0.28102431940318695 1103 28-29 0.30663925943122905 1103 30-31 0.34695011517143826 1103 32-33 0.2610655192546716 1103 34-35 0.45930724739120166 1103 36-37 0.4808113226124888 1103 38-39 0.6279852615132739 1103 40-41 0.668126193892661 1103 42-43 0.6815422385984959 1103 44-45 0.5007092392153751 1103 46-47 0.6955894179255679 1103 48-49 0.7931023463783404 1103 50-51 0.6619002522533464 1103 52-53 0.5918179604384903 1103 54-55 0.7722533750676845 1103 56-57 0.9136047124010602 1103 58-59 0.8712410382712861 1103 60-61 0.7819128100747577 1103 62-63 0.8634572974994796 1103 64-65 0.8664817246084482 1103 66-67 0.9159243799274641 1103 68-69 0.9151392871672783 1103 70-71 1.1316494087750222 1103 72-73 1.1218979606568347 1103 74-75 1.0985880158367287 1103 76-77 1.0571523993425949 1103 78-79 1.1371568759537816 1103 80-81 1.2954840947524708 1103 82-83 1.2461089465528516 1103 84-85 1.4994066547939546 1103 86-87 1.4543047457159268 1103 88-89 1.450477286070587 1103 90-91 1.5397656847342134 1103 92-93 1.6960904879189336 1103 94-95 1.5864481296598711 1103 96-97 1.6803363382730048 1103 98-99 1.760150518728146 1103 100-101 1.7658263592640218 1104 1 0.05935834939754869 1104 2 0.12616086111238545 1104 3 0.11746726760706849 1104 4 0.13532563105398765 1104 5 0.18908745256162263 1104 6 0.2290052064587229 1104 7 0.20908212579681162 1104 8 0.2885272642173078 1104 9 0.2995286751547326 1104 10-11 0.26815402348352535 1104 12-13 0.28666736650976077 1104 14-15 0.3681837097999079 1104 16-17 0.22286238380018375 1104 18-19 0.41273028051256233 1104 20-21 0.43098775477325546 1104 22-23 0.3789601031003613 1104 24-25 0.35084904843363063 1104 26-27 0.34454810719869755 1104 28-29 0.2588517059048385 1104 30-31 0.32555630157432347 1104 32-33 0.27095107513218153 1104 34-35 0.3002123707893176 1104 36-37 0.3464537754751049 1104 38-39 0.3976162927337086 1104 40-41 0.4711711763753428 1104 42-43 0.4915804208688286 1104 44-45 0.4944360868608797 1104 46-47 0.5109031112610367 1104 48-49 0.5118572436161486 1104 50-51 0.35004057656153975 1104 52-53 0.48538655614619586 1104 54-55 0.44414871046402027 1104 56-57 0.5523530866855353 1104 58-59 0.48922523584756306 1104 60-61 0.7298308673151297 1104 62-63 0.6672362796597966 1104 64-65 0.6324846141740892 1104 66-67 0.5716410274776926 1104 68-69 0.6228721345011508 1104 70-71 0.6578129924793323 1104 72-73 0.6627312690621601 1104 74-75 0.5555098471034192 1104 76-77 0.5908232951391987 1104 78-79 0.5120169628461291 1104 80-81 0.9026969363536423 1104 82-83 0.8910124930124859 1104 84-85 1.0498806292816631 1104 86-87 0.9552344116701725 1104 88-89 1.0716592264639182 1104 90-91 1.1483529898436835 1104 92-93 1.254061170793122 1104 94-95 1.341066769054848 1104 96-97 1.4299220124209029 1104 98-99 1.5973075111689283 1104 100-101 1.5354546000355604 1105 1 0.18201563649849462 1105 2 0.10422073506315499 1105 3 0.0960966151908238 1105 4 0.11267162589567903 1105 5 0.23482369980733608 1105 6 0.20742111460054247 1105 7 0.252107488423178 1105 8 0.2332493911877478 1105 9 0.21973287988910783 1105 10-11 0.33229529187513407 1105 12-13 0.2605673215810924 1105 14-15 0.36529673198244694 1105 16-17 0.3738662621510187 1105 18-19 0.3802030601934945 1105 20-21 0.5845525669159883 1105 22-23 0.5089346510693531 1105 24-25 0.5342515854884624 1105 26-27 0.4338204552475915 1105 28-29 0.3468400221890491 1105 30-31 0.44523992230396914 1105 32-33 0.3817391600960178 1105 34-35 0.4147546586202182 1105 36-37 0.4443993925538834 1105 38-39 0.27363547738598726 1105 40-41 0.3753283791643156 1105 42-43 0.5253641725366833 1105 44-45 0.4471832426049005 1105 46-47 0.30610819306628656 1105 48-49 0.4167755175859327 1105 50-51 0.45236441829042917 1105 52-53 0.3068123225646815 1105 54-55 0.3505133026034244 1105 56-57 0.5624079403957651 1105 58-59 0.5731837792535188 1105 60-61 0.5712161639857669 1105 62-63 0.5716566037120288 1105 64-65 0.6124151954287669 1105 66-67 0.5450904510440786 1105 68-69 0.6729434884609233 1105 70-71 0.6758912905770522 1105 72-73 0.6366463889718936 1105 74-75 0.5919402025830749 1105 76-77 0.6925854719951623 1105 78-79 0.7383533076447577 1105 80-81 0.8701760535789766 1105 82-83 0.806591648376255 1105 84-85 0.7387182896866094 1105 86-87 0.7039326687322891 1105 88-89 0.6877193561798727 1105 90-91 1.052830578136426 1105 92-93 1.2477589355638443 1105 94-95 1.1780001671216667 1105 96-97 1.159061588914497 1105 98-99 1.3974038192098028 1105 100-101 1.7110134073549723 1106 1 0.036325655868971296 1106 2 0.13909088700437877 1106 3 0.13471007949539882 1106 4 0.08105035106659386 1106 5 0.14089205748020106 1106 6 0.04638647002586538 1106 7 0.18402126098531824 1106 8 0.01765703917430983 1106 9 0.11780841651718532 1106 10-11 -0.0995325072881812 1106 12-13 -0.2962955549641606 1106 14-15 -0.18378161201228949 1106 16-17 -0.17926024106034077 1106 18-19 -0.1142659452789303 1106 20-21 0.06448183585983713 1106 22-23 -0.08371256514492842 1106 24-25 -0.12361156979408605 1106 26-27 -0.044676315751502216 1106 28-29 0.2273981553784168 1106 30-31 0.33812341835167103 1106 32-33 0.407601863753591 1106 34-35 0.18090148613123347 1106 36-37 0.2714217374048289 1106 38-39 0.16098068704464907 1106 40-41 0.13242988775635922 1106 42-43 0.16164835754767637 1106 44-45 0.03572255338286112 1106 46-47 0.23151597865733464 1106 48-49 0.37200160645490854 1106 50-51 0.24893300942046892 1106 52-53 0.32302060165009294 1106 54-55 0.21355144491939626 1106 56-57 0.26935896613721155 1106 58-59 0.10487138649974526 1106 60-61 -0.08546112292427921 1106 62-63 0.310924218555904 1106 64-65 0.23484766632306275 1106 66-67 0.41055231402059533 1106 68-69 0.3669349946184255 1106 70-71 0.204425520574123 1106 72-73 0.29802022271510253 1106 74-75 0.2774696211276897 1106 76-77 0.402061951625587 1106 78-79 0.33263119578054656 1106 80-81 0.17642564929230176 1106 82-83 0.03901761540057791 1106 84-85 -0.1022683226909713 1106 86-87 -0.06116824710198543 1106 88-89 0.2603017218714321 1106 90-91 0.6074716715392903 1106 92-93 0.6401325893627252 1106 94-95 0.5709731648882403 1106 96-97 0.4382663761391541 1106 98-99 0.5440960779579811 1106 100-101 0.3381002028523632 1107 1 0.24661643993218263 1107 2 0.3277639387167923 1107 3 0.4279910996032932 1107 4 0.41049273206806447 1107 5 0.39318414520897704 1107 6 0.35244929587717877 1107 7 0.36929119368826946 1107 8 0.40684911013870106 1107 9 0.5518244041219518 1107 10-11 0.4770512068883477 1107 12-13 0.4944173116527253 1107 14-15 0.6703170469406743 1107 16-17 0.6280800885421272 1107 18-19 0.6411628274115628 1107 20-21 0.8104044968370232 1107 22-23 0.6672824950871288 1107 24-25 0.6059252020092529 1107 26-27 0.5724692814095889 1107 28-29 0.6370766227175366 1107 30-31 0.6786064774674827 1107 32-33 0.8329420194645749 1107 34-35 0.8221596900091157 1107 36-37 0.7761231854940505 1107 38-39 0.6120536600373185 1107 40-41 0.6991736341704993 1107 42-43 0.6793332265928029 1107 44-45 0.6931026853946207 1107 46-47 0.8559466662367967 1107 48-49 0.8016401099884405 1107 50-51 0.624325957339444 1107 52-53 0.6089595220025856 1107 54-55 0.6813160489413903 1107 56-57 0.7225967320376085 1107 58-59 0.758506603796441 1107 60-61 0.817812644762931 1107 62-63 0.8084931217587368 1107 64-65 0.6473385942892946 1107 66-67 0.8541810129894323 1107 68-69 0.9543900509561496 1107 70-71 0.9497124883958676 1107 72-73 0.8683491796056302 1107 74-75 0.9043212531566276 1107 76-77 0.9400945984519602 1107 78-79 1.0073202996562465 1107 80-81 1.2145581275376394 1107 82-83 1.21435295525432 1107 84-85 1.2462749195419605 1107 86-87 1.362681465872921 1107 88-89 1.2468740706248802 1107 90-91 1.2396388385344785 1107 92-93 1.4154990110194028 1107 94-95 1.6396873778626286 1107 96-97 1.583142069752757 1107 98-99 1.8814134268103473 1107 100-101 1.6077885986668896 1108 1 0.22425348150494884 1108 2 0.18518462599203644 1108 3 0.26297623339912946 1108 4 0.2636618743312198 1108 5 0.21423418529407456 1108 6 0.2079551571385636 1108 7 0.19656967882942666 1108 8 0.28446542529802343 1108 9 0.33811088313566984 1108 10-11 0.3818030791042233 1108 12-13 0.30575618535627314 1108 14-15 0.23639984948844273 1108 16-17 0.18464241823308924 1108 18-19 0.35214693225144345 1108 20-21 0.2007575612951129 1108 22-23 0.2538681393629574 1108 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0.29427294432355566 2316 86-87 0.4593627508960658 2316 88-89 0.6408877231256618 2316 90-91 0.6336557540612624 2316 92-93 0.733020808033082 2316 94-95 0.7640119314806739 2316 96-97 0.9558720913322354 2316 98-99 1.133349488903555 2316 100-101 1.5358116834881663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8513.0 3 1942.0 4 1954.0 5 2073.0 6 2464.0 7 2970.0 8 3669.0 9 4347.0 10 5450.0 11 6582.0 12 8448.0 13 10478.0 14 11996.0 15 14065.0 16 17358.0 17 21949.0 18 19316.0 19 14837.0 20 14852.0 21 15743.0 22 17132.0 23 18993.0 24 21012.0 25 23982.0 26 27308.0 27 31867.0 28 36945.0 29 43722.0 30 52716.0 31 63879.0 32 79117.0 33 100809.0 34 132839.0 35 178820.0 36 243095.0 37 301909.0 38 202861.0 39 21489.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78442432094413 18.846621055727823 14.75937497894966 22.609579644378382 2 15.051023843294972 19.802401834928276 41.072454530764674 24.074119791012084 3 19.352382897244446 24.365305954143484 29.755457726428396 26.52685342218367 4 12.524722060915158 16.189057489837655 36.81851973416596 34.46770071508123 5 13.460962266797589 37.59489409454341 34.05246177584514 14.891681862813858 6 32.088862275583395 35.74389328363523 17.370396099057565 14.796848341723814 7 28.6241585153093 32.994272746435605 20.28729330086245 18.094275437392643 8 26.494846747934663 35.184792748670205 19.33579559215306 18.98456491124207 9 26.485019307227414 16.782401631820957 20.3789229107012 36.35365615025043 10-11 25.067408491815947 26.81700157384601 26.75370010804422 21.361889826293826 12-13 25.834303983942565 24.105354487209137 27.75659867206587 22.30374285678243 14-15 22.813616796051946 25.297466530459833 26.36695556420242 25.5219611092858 16-17 21.899813801452307 28.889869291030042 26.858979335969398 22.351337571548257 18-19 21.739207214914426 27.119194707552825 29.72241898315879 21.41917909437396 20-21 22.516696058306437 25.980322362572128 30.00665936453518 21.49632221458625 22-23 22.193466321820104 25.879945968930734 30.02312213583617 21.90346557341299 24-25 21.987292669164553 26.133839301906885 30.076436274000034 21.802431754928524 26-27 21.646229001265837 26.429113610397593 30.05494456210849 21.86971282622808 28-29 21.606397219087935 26.64660410548964 30.018544086006433 21.728454589415993 30-31 21.711765655695796 26.285410891932866 30.047285538720992 21.95553791365035 32-33 21.5557240716159 26.296093308682018 30.013077905348485 22.135104714353595 34-35 22.09976438796234 26.393509946584327 29.847433847119888 21.659291818333447 36-37 22.017076030997185 26.175819999282474 29.86438732129083 21.94271664842951 38-39 21.711026338917385 26.589179700111202 29.806942102808765 21.892851858162643 40-41 21.98748113987988 26.41167858992438 29.79643961286591 21.804400657329833 42-43 21.659247084556892 26.40288400927067 29.58292914058508 22.35493976558736 44-45 21.796823058389307 26.675809791608508 29.42687574207306 22.100491407929123 46-47 22.019832095952857 26.84320546943846 28.951171493982763 22.185790940625918 48-49 22.109591299170084 26.93997490403991 28.764646318530307 22.185787478259698 50-51 22.0375814904768 27.16242703140313 28.677779870871095 22.12221160724898 52-53 22.201529417009656 27.166765223862228 28.416212504454702 22.215492854673418 54-55 21.97883267758304 27.128301420339163 28.508726566281794 22.384139335796004 56-57 22.0885417031562 27.446218516831895 28.292850387080954 22.172389392930956 58-59 22.240079722607476 27.0485176297433 28.496180191925003 22.21522245572422 60-61 22.135588148099995 27.097594750920344 28.804257367647974 21.96255973333169 62-63 21.994908203341193 27.058177349771682 28.95046497323094 21.996449473656188 64-65 21.900086168848908 27.091093783933363 28.787232999166335 22.221587048051394 66-67 21.909914312728 27.32484661871826 28.351591875485845 22.413647193067902 68-69 21.865763803905335 27.222550869398667 28.60806260107535 22.303622725620645 70-71 21.97939482527861 27.15792742118089 28.533716845307623 22.32896090823288 72-73 21.93806306905897 27.240401501919152 28.447631092158865 22.373904336863013 74-75 21.793405023868196 27.576565446387928 28.201718196107095 22.428311333636778 76-77 22.005036679311612 27.308403284098752 28.239482723057748 22.447077313531885 78-79 22.05482189456526 27.33244451345239 28.191574783486782 22.421158808495566 80-81 21.97499995796334 27.574198907383153 28.10063800439087 22.35016313026264 82-83 22.101385117301806 27.4868005348151 28.091885761164487 22.31992858671861 84-85 22.004407292065054 27.467488165734355 27.974172652094303 22.553931890106288 86-87 21.94898613358917 27.492019976956563 28.17407658132226 22.384917308132003 88-89 22.008129561880168 27.392941527232296 27.988395787378273 22.610533123509263 90-91 22.054510369009048 27.278023731498124 28.123131176749833 22.544334722742995 92-93 22.141792096656634 27.116052245830357 28.149093017384175 22.593062640128835 94-95 21.96532132056734 27.131983877367567 28.118127649462764 22.78456715260233 96-97 21.893792250380343 27.103912361049964 28.087390103122946 22.914905285446743 98-99 21.864125047398925 27.110502760698285 27.986286331817965 23.039085860084825 100-101 22.17256367377864 26.51506830276484 28.44915512595774 22.863212897498776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2296.0 1 1368.5 2 451.5 3 490.0 4 438.5 5 407.0 6 683.0 7 1424.0 8 2412.5 9 2956.5 10 2728.5 11 2442.5 12 2482.0 13 2665.0 14 2863.5 15 2961.0 16 3101.0 17 3222.5 18 3368.5 19 3566.0 20 3886.0 21 4428.5 22 5214.5 23 6227.0 24 7331.5 25 8886.0 26 11291.0 27 13653.0 28 15422.0 29 17704.0 30 20381.5 31 23442.0 32 27153.5 33 30574.0 34 34241.0 35 37687.5 36 42271.0 37 47037.0 38 49652.5 39 51357.0 40 52207.5 41 54432.5 42 57136.0 43 59600.5 44 62628.5 45 64957.5 46 65877.0 47 67885.5 48 71715.0 49 71457.0 50 67981.5 51 65079.0 52 62867.0 53 61801.0 54 60575.5 55 55384.0 56 48676.0 57 43369.5 58 38540.5 59 33694.5 60 28231.0 61 23265.0 62 18517.0 63 13922.0 64 10404.5 65 7800.5 66 5637.5 67 4219.0 68 3118.0 69 2214.5 70 1593.5 71 1081.5 72 773.0 73 578.5 74 401.0 75 281.0 76 197.5 77 115.0 78 66.5 79 48.5 80 35.0 81 23.5 82 17.5 83 15.0 84 9.0 85 8.5 86 9.5 87 9.5 88 6.5 89 4.5 90 4.0 91 2.5 92 3.0 93 3.0 94 2.0 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3391874829021649 2 0.29107578319973015 3 0.28162127476983306 4 0.29046040099423387 5 0.2940967503903481 6 0.22030682956766043 7 0.21006910742167725 8 0.21141175950639635 9 0.2225445830421923 10-11 0.2225445830421923 12-13 0.2187124302170565 14-15 0.2206984364257035 16-17 0.20467052716436912 18-19 0.21020896701383546 20-21 0.1987684523752914 22-23 0.20802715737616692 24-25 0.2088663149291164 26-27 0.2074956909259656 28-29 0.21927186858568945 30-31 0.21678236784527277 32-33 0.22178934124453775 34-35 0.2156355191895752 36-37 0.2015656442184562 38-39 0.18903422476107787 40-41 0.18567759454928007 42-43 0.20148172846316126 44-45 0.204362836061621 46-47 0.2031320716506285 48-49 0.22114598712060987 50-51 0.2161110418029132 52-53 0.23935570601961279 54-55 0.20545374088045532 56-57 0.18570556646771175 58-59 0.18576151030457502 60-61 0.17443288333975754 62-63 0.18262865544023046 64-65 0.1801111827813821 66-67 0.18058670539472013 68-69 0.1821811047453241 70-71 0.18433494246456097 72-73 0.1821811047453241 74-75 0.19820901400665844 76-77 0.1899293261508906 78-79 0.1954397940819253 80-81 0.18741185349204229 82-83 0.16587347629967322 84-85 0.16584550438124157 86-87 0.17068464626991672 88-89 0.1765028053036995 90-91 0.18439088630142428 92-93 0.17966363208647573 94-95 0.1780692327358718 96-97 0.18335592531945327 98-99 0.19286637758621364 100-101 0.17336995043935494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787507.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.78081308070182 #Duplication Level Percentage of deduplicated Percentage of total 1 78.70972889122014 45.479121326963075 2 12.215361671671264 14.116270589280134 3 3.8750070452617296 6.71703173306012 4 1.7046969564200882 3.9399510479260167 5 0.8941177331892671 2.5831424806774934 6 0.5392672984233382 1.869558178244037 7 0.3591039508953886 1.4524522784259581 8 0.2548307179870887 1.1779460866586409 9 0.2022614600965446 1.0518148457341445 >10 1.1167238073440744 12.154739279337926 >50 0.08798661598970685 3.516431368748256 >100 0.039173109093378206 4.011437454082096 >500 9.67079115550304E-4 0.328901543251651 >1k 5.802474693301823E-4 0.7108318946150615 >5k 1.934158231100608E-4 0.8903698929955499 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8950 0.500697339926501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6984 0.39071175665326063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4699 0.26288008942062885 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2518 0.14086658122177984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.59438368632962E-5 0.0 16-17 0.0 0.0 0.0 5.59438368632962E-5 0.0 18-19 0.0 0.0 0.0 5.59438368632962E-5 0.0 20-21 0.0 2.79719184316481E-5 0.0 1.118876737265924E-4 0.0 22-23 0.0 1.118876737265924E-4 0.0 2.2377534745318483E-4 0.0 24-25 0.0 1.398595921582405E-4 0.0 3.356630211797772E-4 0.0 26-27 0.0 1.678315105898886E-4 0.0 5.314664502013139E-4 0.0 28-29 0.0 1.678315105898886E-4 0.0 0.0013146801662874607 0.0 30-31 0.0 1.678315105898886E-4 0.0 0.0029370514353230506 0.0 32-33 0.0 1.678315105898886E-4 0.0 0.005090889154559955 0.0 34-35 0.0 1.678315105898886E-4 0.0 0.00618179397339423 0.0 36-37 0.0 1.678315105898886E-4 0.0 0.008867098142832448 0.0 38-39 0.0 2.237753474531848E-4 0.0 0.012587363294241646 0.0 40-41 0.0 2.237753474531848E-4 0.0 0.017146785998600287 0.0 42-43 0.0 2.237753474531848E-4 0.0 0.02086705115000948 0.0 44-45 0.0 2.237753474531848E-4 0.0 0.023160748461404625 0.0 46-47 2.79719184316481E-5 2.237753474531848E-4 0.0 0.02590199646770614 0.0 48-49 5.59438368632962E-5 2.237753474531848E-4 0.0 0.029790093129705226 0.0 50-51 5.59438368632962E-5 2.237753474531848E-4 0.0 0.03429357199720057 0.0 52-53 5.59438368632962E-5 2.237753474531848E-4 0.0 0.0448949290827952 0.0 54-55 5.59438368632962E-5 2.237753474531848E-4 0.0 0.05289489775424656 0.0 56-57 5.59438368632962E-5 2.237753474531848E-4 0.0 0.07574795511290305 0.0 58-59 5.59438368632962E-5 2.237753474531848E-4 0.0 0.10819538049361485 0.0 60-61 5.59438368632962E-5 2.237753474531848E-4 0.0 0.14030714285314688 0.0 62-63 5.59438368632962E-5 2.237753474531848E-4 0.0 0.15546792264310014 0.0 64-65 5.59438368632962E-5 2.237753474531848E-4 0.0 0.18310417805356846 0.0 66-67 5.59438368632962E-5 2.237753474531848E-4 0.0 0.23367740657798824 0.0 68-69 5.59438368632962E-5 2.237753474531848E-4 0.0 0.3065722260108632 0.0 70-71 5.59438368632962E-5 2.237753474531848E-4 0.0 0.37966284887275964 0.0 72-73 5.59438368632962E-5 2.237753474531848E-4 0.0 0.4612289630194455 0.0 74-75 5.59438368632962E-5 2.237753474531848E-4 0.0 0.5567530644635238 0.0 76-77 5.59438368632962E-5 2.237753474531848E-4 0.0 0.6230185392280981 0.0 78-79 5.59438368632962E-5 2.237753474531848E-4 0.0 0.7185985845090397 0.0 80-81 1.118876737265924E-4 2.237753474531848E-4 0.0 0.8423743235690826 0.0 82-83 1.118876737265924E-4 2.237753474531848E-4 0.0 0.9635486742149821 0.0 84-85 1.118876737265924E-4 2.237753474531848E-4 0.0 1.1024851930649782 0.0 86-87 1.118876737265924E-4 2.237753474531848E-4 0.0 1.2650300110712853 0.0 88-89 1.118876737265924E-4 2.237753474531848E-4 0.0 1.494959180579433 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6055 0.0 37.22997 1 GTATCAA 9370 0.0 34.564957 1 TATCAAC 11555 0.0 28.020218 2 ATCAACG 11515 0.0 27.951616 3 TCAACGC 11525 0.0 27.929714 4 CAACGCA 11705 0.0 27.458046 5 AACGCAG 12060 0.0 26.709892 6 GTACATG 8500 0.0 25.457806 1 TACATGG 8590 0.0 24.740513 2 ACATGGG 8700 0.0 23.443378 3 GAGTACT 7335 0.0 22.989347 12-13 ACGCAGA 13880 0.0 22.93375 7 CGCAGAG 13915 0.0 22.705349 8 GTACCTG 1460 0.0 21.824808 1 CATGGGG 4175 0.0 21.410172 4 GTACTTT 7765 0.0 21.379526 14-15 GTATAGG 535 0.0 21.33467 1 GCAGAGT 14735 0.0 20.79635 9 TACCTGG 1465 0.0 20.77001 2 AGTACTT 7795 0.0 20.322546 12-13 >>END_MODULE